Search Results (442)

Background: Kinkeliba (Combretum micranthum G. Don), commonly used in West African traditional pharmacopeia for its anti-inflammatory and gastrointestinal properties, remains poorly studied regarding its potential role in the prevention or treatment of ulcerative colitis. Objective: This study evaluated the therapeutic potential of the ethanolic extract of Combretum micranthum (EECM) in a murine model of chronic DSS-induced colitis. Methods: Male C57BL/6 mice were subjected to three cycles of 1.5% DSS administration over nine weeks to induce chronic colitis. EECM was administered orally at 50, 100, or 200 mg/kg during the final week. Disease severity was evaluated using the Disease Activity Index (DAI), colon length, biochemical and hematological markers, along with histopathological and immunohistochemical assessment of colonic tissue. Results: EECM treatment significantly improved clinical parameters and prevented colon shortening in chronic DSS-induced colitis. These improvements were associated with the restoration of serum biochemical and hematological profiles, along with reduced histopathological damage and preservation of colonic tissue architecture. Immunohistochemical analysis further demonstrated decreased CD3-positive T-lymphocyte infiltration in colonic tissue, suggesting modulation of local immune cell responses. Conclusions: These findings highlight the therapeutic potential EECM in ulcerative colitis and support further investigations to elucidate its mechanisms of action and evaluate its efficacy in future translational studies. Full article

Phellodendri amurensis Cortex is the dried bark of the cork tree (Phellodendron amurense Rupr.) from the Rutaceae family, and possesses traditional efficacy in clearing heat, drying dampness, purging fire, relieving steaming sensations, detoxifying, and healing sores. Clinically, it is commonly used for treating symptoms such as damp-heat diarrhea and dysentery, jaundice with reddish urine, leukorrhea with vaginal itching, painful and difficult urination due to heat strangury, flaccidity and weakness of the lower limbs, bone-steaming and consumptive fever, night sweats and seminal emission, sores, ulcers, swellings, and toxins, eczema, damp sores, and urinary tract infections. Modern pharmacological studies have further revealed its diverse bioactivities, including antioxidant, antibacterial, anti-inflammatory, immunosuppressive, and anticancer effects. To provide an updated and comprehensive review of the research into Phellodendri amurensis Cortex, this study conducted a thorough literature search and analysis based on databases such as SciFinder, Web of Science, and China National Knowledge Infrastructure. The review integrates information on the plant’s botanical characteristics, geographical distribution, traditional applications, chemical components, quality control methods, and pharmacological effects to present a current and holistic overview of its research status. To date, approximately 170 compounds have been isolated and identified from Phellodendri amurensis Cortex, primarily including alkaloids, phenolics, terpenoids, sterols, lignans, flavonoids, and others. Among these, alkaloids exhibit significant antioxidant and anti-inflammatory activities and demonstrate potential pharmacological value in antibacterial, anticancer, hypoglycemic, and multi-organ protective effects. Although substantial foundational research exists, the mechanisms of action and quality control of Phellodendri amurensis Cortex require further in-depth exploration. Future efforts should focus on clarifying its pharmacodynamic material basis, uncovering new targets and pathways, and improving analytical methods for component analysis and quality control to advance the scientific development and rational utilization of this medicinal material. Full article

Brosimum alicastrum Swartz is a forest species with substantial potential for animal and human nutrition. However, its nutritional attributes and commercial applications are poorly disseminated and structurally underdeveloped. This study examines the relationship between scientific research and the commercialization of Brosimum alicastrum products in Mexico, integrating bibliometric analysis with a value network approach to identify market constraints and opportunities. Scientific publications indexed in Scopus from 1961 to 2024 were analyzed to characterize research trends, documented uses, and the geographic distribution of knowledge production. In parallel, companies commercializing Brosimum alicastrum-based products in Mexico were surveyed during 2024 using a value network approach (suppliers, customers, complementors, and competitors). A SWOT analysis was conducted to assess the structural strengths and vulnerabilities affecting market development. The results show that research in Mexico has primarily focused on the species’ properties as a functional food. At the same time, limited attention has been given to silviculture, commercialization strategies, and value-chain governance. Although Brosimum alicastrum products are currently positioned within premium market segments, business continuity is constrained by unstable supply systems that rely almost exclusively on seasonal wild collection from natural distribution areas. Both the value network and the SWOT analysis identified supply instability as the main factor limiting market expansion. Therefore, advancing research on the silviculture of Brosimum alicastrum is essential to support the establishment of managed production systems and commercial plantations capable of ensuring a stable, year-round supply of raw material. These developments would facilitate access to new market niches and enhance the biocultural and ethnobotanical value of Brosimum alicastrum as a functional and medicinal food resource within Mexico’s emerging bioeconomy. Full article

The genus Oncosiphon (Asteraceae), consisting of aromatic herbs, is indigenous to southern Africa. Oncosiphon species have been documented in Khoi-San ethnobotany as herbal remedies for typhoid fever, pneumonia, and as diuretics. Research on the biological properties and comprehensive phytochemical profiling of these important Oncosiphon species is currently limited. This study was therefore undertaken to address the knowledge void in chemical profiling, through the application of various analytical techniques to analyse the volatile and non-volatile constituents of three South African Oncosiphon species. The aerial parts of Oncosiphon suffruticosus (n = 28), O. grandiflorus (n = 16), and O. africanus (n = 4) were collected from various locations in the Western Cape Province of South Africa. The stems and leaves (SL) were separated from the flowers (F) and analysed as distinct samples. The methanol: chloroform (1:1, v/v) extracts were prepared and analysed using ultra–high–performance liquid chromatography quadrupole time-of-flight time–of–flight mass spectrometry (UHPLC–QToF–MS) and a semi–automated high–performance thin–layer chromatography (HPTLC) system. Multivariate data analysis was performed on the UHPLC–QToF–MS data to determine interspecies chemical variation. Two-dimensional (2D) gas chromatography (GCxGC–ToF–MS) was used to determine the headspace volatile profiles of the intact aerial parts. The results show that the non-volatile profiles of the Oncosiphon species are characterised by amino acids, phenolic acids, flavonoids, sesquiterpene lactones, and fatty acid derivatives. The HPTLC profiles of O. grandiflorus and O. africanus are chemically more closely related, and O. suffruticosus has a distinct profile, which is supported by the chemometrics results of the flowers. The major headspace volatile compounds in Oncosiphon flowers are α-pinene, α-ocimene, eucalyptol, o-cymene, and artemisia alcohol, whereas the stems and leaves mainly consist of α-ocimene, eucalyptol, and yomogi alcohol. Full article

Traditional markets play an important role in the exchange of plant resources and the preservation of traditional food knowledge. This study documents the diversity of food plants traded in Renmin Market, located in Youjiang District, Baise City, Guangxi, China, and evaluates their cultural importance using the Cultural Food Significance Index (CFSI). Field surveys were conducted through market observations and interviews with vendors and local informants. All edible plant species were recorded, including their scientific names, vernacular names, used parts, and modes of consumption. A total of 104 food plant taxa were documented, representing a wide range of plant families and growth forms. The recorded plants were used in four main utilization categories: vegetables, spices, fruits, and beverages. Frequently used plant parts included fruits, leaves, shoots, and underground organs such as roots, rhizomes, and tubers. The CFSI values showed considerable variation in cultural importance among species, ranging from 21.6 to 1764. The highest CFSI values were recorded for Cucurbita pepo, Allium cepa, Cucurbita maxima, and Houttuynia cordata, reflecting their frequent consumption and versatility in local cuisine. Comparative analysis with previous studies in Baise City indicated that 38 species were shared among three markets, while 30 species were recorded exclusively in Renmin Market. These findings highlight the diversity of food plants available in local markets and their importance in maintaining regional culinary traditions and plant-based dietary diversity. Full article

The escalating antimicrobial resistance (AMR) crisis necessitates the development of innovative anti-infectives with novel mechanisms of action. Nevertheless, research on natural products remains constrained by low-throughput screening and limited mechanistic insights. Artificial intelligence (AI) is catalyzing a pivotal paradigm shift—from the mere isolation of active compounds to precisely deciphering their modes of action. This review highlights AI’s transformative role in bridging ethnopharmacological knowledge and modern pharmacology to decode the mechanisms of plant-derived anti-infectives. Case studies on berberine, baicalein, danshensu derivatives, and rosmarinic acid derivatives from Coleus amboinicus illustrate AI’s capacity to map traditional therapeutic concepts to specific pathways (e.g., biofilm inhibition, inflammasome modulation) and to predict precise binding interactions and pharmacophores with high precision. Leveraging statistical correlations between ethnobotanical usage patterns and chemical similarity, we propose a “Knowledge–Data–Mechanism” three-layer framework centered on deep mechanistic insight. Integrating Chinese initiatives, such as the CNDR (China’s National Drug Repository) database and the TCM-AI platform, with global traditional medicine wisdom, this strategy provides an actionable roadmap for modernizing anti-infective discovery. Validated applications of this paradigm have demonstrated order-of-magnitude acceleration in mechanistic characterization, rapidly yielding structurally novel agents with well-defined, target-specific actions—a critical advancement in addressing the urgent global threat of antimicrobial resistance. Full article

Understanding how ecological dominance aligns with culturally and economically valued plant use is critical for participatory forest management. This study integrated vegetation structure and ethnobotanical valuation to assess angiosperm importance across three forest strata (Mixed Deciduous Forest (MDF), Dry Dipterocarp Forest site 1 (DDF1), and Dry Dipterocarp Forest site 2 (DDF2)) within the Ngao Model Forest, Northern Thailand. Fifteen 10 × 10 m vegetation plots (five per forest stratum) were surveyed to calculate the Importance Value Index (IVI), and 198 semi-structured interviews were conducted to derive the Use Value Index (UVI) and a standardized Socio-Economic Value Index (SEVI). A total of 112 angiosperm species were recorded across forest types, with strong structural dominance by dipterocarps in DDF sites and greater compositional heterogeneity in MDF. Spearman rank correlation analysis supported the working hypothesis that ecological dominance is only weakly associated with cultural and socio-economic importance. IVI showed weak but significant positive correlations with UVI (ρ = 0.288, p < 0.05) and SEVI (ρ = 0.300, p < 0.05), indicating partial but limited alignment between structural abundance and livelihood value. Several species with moderate or low IVI exhibited disproportionately high UVI and SEVI scores, reflecting their importance in food, medicinal, and commercial use categories. Conversely, certain canopy dominants showed limited ethnobotanical significance. These findings demonstrate that ecological abundance alone is an insufficient proxy for community-defined species value. Integrating structural, cultural, and socio-economic indices provides a more comprehensive framework for identifying priority species in community-managed forest systems. The IVI–UVI–SEVI comparative approach offers practical insights for model forest governance by distinguishing ecological dominants, multipurpose livelihood species, and culturally significant taxa occurring outside forest interiors. This multidimensional valuation framework strengthens participatory forest management and biodiversity prioritization in heterogeneous tropical landscapes. Full article

This study examines 36 wild Asteraceae species that are traditionally used as food in Sicily and Bulgaria, highlighting their ethnobotanical, nutritional, and pharmacological relevance. Some taxa, such as Cichorium intybus, Silybum marianum, Artemisia vulgaris, Taraxacum officinale, and Tussilago farfara, are integral to the Mediterranean and Balkan diets, enhancing nutrition through their fiber, minerals, and bioactive compounds. This ethnobotanical survey revealed a clear geographic pattern in species usage: 13 species were found to be consumed solely in Bulgaria, 18 solely in Sicily, and five species in both regions. The distribution highlights the existence of shared culinary traditions that have been distinctly adapted to the unique ecological conditions present in each locale. The main metabolite classes identified include flavonoids, phenolic acids, lignans, and sesquiterpene lactones, all of which are associated with antioxidant, anti-inflammatory, hypolipidemic, and anticancer properties. Specific taxa within the investigated group were found to contain alkaloids that warrant toxicological attention. Some species within the studied group contain alkaloids that may pose toxicity risks. T. farfara is known to accumulate pyrrolizidine alkaloids, which are associated with liver damage and potential genotoxic effects, highlighting the importance of thorough toxicological evaluations before recommending these plants for consumption. This study also demonstrates how common culinary processes, such as boiling and blanching, significantly impact the concentration, stability, and safety profile of these bioactive compounds. Overall, the research supports the promotion of wild Asteraceae species as valuable and sustainable nutritional and nutraceutical resources. This approach aligns with efforts to preserve biodiversity and maintain traditional Mediterranean and Balkan food practices, integrating both ecological and cultural sustainability. Full article

Background/Objectives: Cancer has been associated with significant morbidity and mortality worldwide, particularly related to chemotherapy resistance. Therefore, it is essential to investigate alternative sources of non-toxic antitumor compounds. The cactus Lophocereus marginatus is native to Mexico and is commonly used to treat gastrointestinal infections and diabetes in traditional medicine. Methods: The in vitro antitumor activity of L. marginatus extract fractions against murine L5178Y-R lymphoma cells was evaluated. The crude extract and its solvent-derived fractions were evaluated for cytotoxicity, selectivity, and hemolytic activity. Results: The crude extract exhibited an IC₅₀ of 9.09 μg/mL, demonstrating a high selectivity index (SI: 330.03), with no hemolytic activity observed at 1000 μg/mL. The LM-HP, LM-CP, and LM-MP partitions showed varying IC₅₀ values (6.74, 7.93, and 45.38 μg/mL, respectively) and selectivity indices of 445.1, 378.31, and 66.1, respectively. Only LM-HP induced hemolysis at 200 μg/mL. The most promising fraction, CP-F8, exhibited an IC₅₀ of 11.2 μg/mL, high selectivity index (354.29), and antioxidant activity, without hemolytic effects. Phytochemical analysis of CP-F8 identified phenolic compounds, triterpenes, and sterols, which are known for their anti-cancer and anti-inflammatory properties. In vivo tests showed no significant liver damage or changes in body weight, indicating the safety of CP-F8. Conclusions: These results suggest that CP-F8 is a promising antitumor candidate with selective cytotoxicity and minimal toxicity to normal cells. Full article

Helichrysum Mill. is one of the largest genera in the Asteraceae family, and in Madagascar, a plant paradise with ca 14,000 species, 111 Helichrysum species are known, 110 of which are endemic. In this work, the essential oil of endemic Helichrysum manopappoides Humbert, obtained by steam distillation, was investigated chemically and biologically. The spectrometric data obtained by GC-MS (Gas Chromatography–Mass Spectrometry) analysis highlighted the presence of three major compounds, such as eucalyptol (7.38 ± 0.36%), α-humulene (14.75 ± 0.79%), and β-caryophyllene (19.78 ± 0.89%), which were also structurally confirmed by NMR (Nuclear Magnetic Resonance) spectroscopic investigation. Biological results showed potential antimicrobial, antioxidant, and antibiofilm effects of both the H manopappoides essential oil and the main components identified by GC-MS, enhancing an interesting approach for intestinal infections, being active against Escherichia coli, Listeria monocytogenes, Shigella sonnei, and Salmonella enterica ser. typhimurium strains. Full article

Spices and pickled vegetables play a central role in everyday cuisine in China, contributing to flavor, food preservation, and dietary continuity, yet their ethnobotanical diversity and use patterns remain insufficiently documented in urbanizing regions. This study aimed to document the diversity, utilization forms, and cultural importance of spice plants and species used for pickled products in Youjiang District, Baise city, Guangxi Zhuang Autonomous Region, southern China. Ethnobotanical data were collected in 2025 through field surveys conducted in traditional markets, local households, and local restaurants. Semi-structured interviews, informal conversations, and participant observation were carried out with 40 informants selected using snowball sampling to capture practical knowledge related to culinary use, fermentation, and trade. The cultural importance of recorded species was evaluated using the Use Value (UV) index. A total of 68 plant taxa belonging to 55 genera and 27 families were documented. Zingiberaceae and Apiaceae were the most species-rich families, with native and introduced species nearly equally represented. Herbs dominated the recorded growth forms, while fruits, leaves, and roots were the most frequently used plant parts. Dried use was the predominant form, followed by fresh use and pickled products. Sixty species were used as spices and 13 species as pickled vegetables, with several taxa exhibiting multifunctional use. Species with the highest UVs, including Allium sativum, A. cepa, A. fistulosum, and Houttuynia cordata, were characterized by frequent daily use. These findings highlight the role of ethnobotanical knowledge in sustaining culturally embedded and resilient urban food systems. Full article

This study documents the ethnomedicinal knowledge of Konak (Malatya, Eastern Anatolia, Türkiye), a region with rich plant diversity but no prior comprehensive research. The aim of the study is to systematically document and analyze the ethnomedicinal practices of Konak village, focusing on plant taxa (species, subspecies and varieties) used, preparation methods, and therapeutic applications. Data were collected through semi-structured interviews with 68 local informants. Quantitative analysis was performed using Informant Consensus Factor (F_IC) and Use Value (UV) indices. Plant specimens were collected, identified, and deposited in the herbarium. The study documented 86 plant taxa from 35 families used in 230 therapeutic applications. Lamiaceae, Asteraceae, and Rosaceae were the most represented families. High F_IC values were recorded for colds (F_IC = 0.95), stomach pain (F_IC = 0.92), and inflammation (F_IC = 0.90), indicating strong community consensus. The most frequently cited species were Origanum vulgare subsp. gracile, Mentha spp., and Rosa canina. There are novel or locally specific uses, with 13 taxa having no previously recorded ethnomedicinal applications in the reviewed literature. The findings reveal Konak as a significant repository of ethnomedicinal knowledge. High-F_IC taxa represent prime candidates for phytochemical and pharmacological research to validate traditional uses and support evidence-based phytotherapy. This study enriches regional ethnopharmacological data and highlights candidate taxa for pharmacological validation. Full article

Triterpenoids with diverse structural features have shown considerable potential as pharmaceutical precursors for anti-inflammatory therapies. Beesioside O (BO), a representative triterpenoid (cycloartane triterpene saponin), has previously been reported to exhibit notable anti-HIV and anticancer activities. However, its anti-inflammatory mechanisms have not been fully elucidated. In this study, we investigated the anti-inflammatory activity and underlying molecular mechanisms of BO in LPS-induced RAW264.7 macrophages. In addition, NP AI Engine predictions, molecular docking, density functional theory (DFT) calculations, and molecular dynamics simulations were conducted to characterize the anti-inflammatory properties of BO further. The experimental results indicated that BO inhibited the mRNA expression levels of iNOS and COX-2. Moreover, it can regulate the phosphorylation of ERK at 3 h. Potential signaling pathways and targets were subsequently analyzed. The structural and electronic properties of BO were calculated using the B3LYP/6-311+G (d,p) basis set. The BO–ERK2 kinase complex was also constructed for simulation. Furthermore, a BO derivative was prepared through hydrolysis followed by acylation, and its anti-inflammatory activity was evaluated. Overall, this study provides deeper insight into the anti-inflammatory effects of BO and supports its potential for further development as an anti-inflammatory agent. Full article

Greece lies within the Mediterranean global biodiversity hotspot and harbors exceptional plant richness and endemism, including numerous medicinal and aromatic plants (MAPs). These taxa underpin long ethnobotanical traditions and contemporary bioeconomy niches (culinary herbs, essential oils, phototherapeutics). The aim of this review is to map (i) the biodiversity knowledge base for Greek MAPs, (ii) recent ethnobotanical evidence, and (iii) sustainability pathways (conservation, cultivation, value chains, and regulation) in a Mediterranean context. The information is presented and analyzed in a critical manner. A total of 148 research studies were systematically reviewed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. Key findings highlight that (i) Greece is a regional plant-diversity hotspot with many MAP endemics in Lamiaceae; (ii) contemporary ethnobotanical knowledge persists and adapts; and (iii) strong sustainability levers exist through Natura 2000 coverage, Good Agricultural and Collection Practice (GACP) and European Medicines Agency (EMA) frameworks, and Protected Designation of Origin (PDO)/United Nations Educational, Scientific and Cultural Organization (UNESCO) recognitions (e.g., Krokos Kozanis saffron, Chios mastic), although threats from climate and land-use change remain significant. In conclusion, Greek MAPs combine high biodiversity value, living ethnobotanical traditions, and tangible bioeconomic opportunities. Their sustainable prospects depend on integrating habitat protection, GACP and FairWild Standard (FairWild)-aligned wild collection and cultivation, domestication of priority endemics, and climate-resilience planning, all supported by traceable value chains and Access and Benefit-Sharing (ABS)/Nagoya Protocol compliance. The review concludes with practical recommendations and a prioritized list of flagship taxa for “conservation through use.” Full article