Search Results (1,999)

Stapedotomy is performed to restore ossicular chain sound transmission by inserting a piston prosthesis that couples the long process of the incus to the oval window, thereby addressing conductive hearing loss associated with otosclerosis. This study investigates the effects of crimping force, prosthesis material, and loop geometry on incus to optimize fixation while minimizing complications such as incudal necrosis. Finite element analyses were performed to quantify interface pressures and von Mises stresses for titanium prostheses with loop-band widths of 0.2, 0.3, and 0.5 mm under crimping forces of 300–500 mN and for polytetrafluoroethylene (PTFE) prostheses with loop outer diameters (OD) of 1.2, 1.4, and 1.8 mm. The analysis results showed that PTFE prostheses generated significantly lower interface pressures and stress compared to titanium. For PTFE prostheses, the equivalent von Mises stresses remained well below the critical threshold, with values ranging from 3.5 MPa up to peaks of approximately 43 MPa depending on the loop’s outer diameter. In contrast, titanium prostheses exhibited a marked dependency on crimping force and band width. At a force of 300 mN, stresses were modest (approximately 16–24 MPa). However, when increasing the force to 400 mN, stresses approached the critical threshold (up to approximately 53 MPa). With crimping forces of 500 mN, especially with band widths greater than 0.3 mm, stresses exceeded the cortical bone strength threshold (approximately 61–64 MPa), indicating an increased risk of mechanical overload and potential incudal necrosis. These findings highlight the importance, in a clinical context, of controlling the crimping force and selecting the material and geometry of the prosthesis to achieve secure coupling while preserving the incus’s structural integrity. Full article

University years represent a period of major transition during which health-related behaviors, such as sleep and physical activity, may influence students’ academic functioning. This cross-sectional, single-center study, conducted at an Italian university, examined the associations between sleep, physical activity, and academic well-being. Students completed an online survey assessing sleep, physical activity, and several indicators of academic functioning (i.e., academic stress, self-efficacy, dropout intention, and motivation). Nonparametric tests (Kruskal–Wallis, Jonckheere–Terpstra) were used to explore differences in these indicators across sleep quality and physical activity categories, while linear regressions tested associations between sleep duration and Metabolic Equivalent of Task–minutes/week with the same academic outcomes. A total of 2192 students (15.55%) accessed the survey, and 1246 (8.84%) completed all questionnaires. Most participants were female (62.7%) and Italian (94.5%). Both sleep and physical activity showed significant but small associations with academic stress, dropout intention, and self-efficacy, whereas associations with academic motivation were weaker. These findings suggest that maintaining regular physical activity and healthy sleep habits may contribute to students’ academic adjustment, although the cross-sectional design limits causal interpretation and underscores the need for integrative models to better understand the underlying psychological mechanisms. Full article

The paper introduces a real-time digital-twin controller that manages evacuation routes while operating GEEM for emergency energy management during building fires. The system consists of three interconnected parts which include (i) a physics-based hazard surrogate for short-term smoke and temperature field prediction from sensor data (ii), a router system that manages path updates for individual users and controls exposure and network congestion (iii), and an energy management system that regulates the exchange between PV power and battery storage and diesel fuel and grid electricity to preserve vital life-safety operations while reducing both power usage and environmental carbon output. The system operates through independent modules that function autonomously to preserve operational stability when sensors face delays or communication failures, and it meets Industry 5.0 requirements through its implementation of auditable policy controls for hazard penalties, fairness weight, and battery reserve floor settings. We evaluate the controller in co-simulation across multiple building layouts and feeder constraints. The proposed method achieves superior performance to existing AI/RL baselines because it reduces near-worst-case egress time (\(T_{95}\) and worst-case exposure) and decreases both event energy \(E_{\mathrm{event}}\) and CO₂-equivalent \(CO_{\mathrm{2event}}\) while upholding all capacity, exposure cap, and grid import limit constraints. A high-VRE, tight-feeder stress test shows how reserve management, flexible-load shedding, and PV curtailment can achieve trade-offs between unserved critical load \(U_{\mathrm{energy}}\) and emissions. The team delivers implementation details together with reporting templates to assist researchers in reaching reproducibility goals. The research shows that emergency energy systems, which integrate evacuation systems, achieve better safety results and environmental advantages that enable smart-city integration through digital thread operations throughout design, commissioning, and operational stages. Full article

Acrolein, a ubiquitous environmental pollutant, is also formed endogenously as a metabolite under oxidative stress conditions. Its adduct to cytosine, 3,N⁴-α-hydroxypropanocytosine (HPC), has recently been shown to be an in vitro substrate for the AlkB dioxygenase. Using a set of indicator plasmids modified with acrolein, we provide evidence that HPC is a mutagenic non-instructional lesion that predominantly induces C→A transversion, and to a lesser extent C→T and C→G base substitutions. HPC is efficiently repaired in vivo by AlkB, even without induction of the adaptive response. However, the mutation frequency did not differ between the wild-type and AlkA-deficient strains, and AlkA glycosylase fails to excise in vitro the acrolein-modified cytosine from the T₂₂(HPC)₃ oligodeoxynucleotide, both indicating that HPC is not a substrate for AlkA. Based on molecular modeling, we further examined the potential differences in the hydrolytic suspensibility of a known AlkA substrate, the acrolein adduct to adenine (HPA), and the cytosine adduct (HPC) at the glycosylase active site. Analysis of both structural and electrochemical properties indicates that, despite an identical type of modification within an equivalent chemical context, including comparable geometry and topology, the glycosidic bond in HPC is considerably less susceptible to hydrolysis than that in HPA. Full article

The study presents a comprehensive analysis of the effects of high temperatures (500 °C and 700 °C) on the microstructure, mechanical properties, and acoustic emission (AE) parameters of cold-drawn prestressing steel. The investigations included mechanical testing, AE signal acquisition, and numerical verification using the finite element method (FEM). It was demonstrated that increasing temperature leads to significant microstructural changes (pearlite spheroidisation, carbide coarsening), resulting in strength degradation and a shift in the failure mechanism from quasi-brittle (initial state) to transitional (500 °C), and finally to ductile (700 °C). For the first time, AE parameters (Counts to Peak and RA-value) were correlated with local axial strains ε₂₂ and von Mises equivalent stress, enabling the identification of the moment of onset load-bearing capacity loss and the determination of critical material damage thresholds. A multi-criteria diagnostic indicator was proposed to assess the condition of prestressing steel after fire exposure. The results confirm the high potential of AE as a non-invasive tool for evaluating the safety of prestressing tendons and cables in reinforced concrete structures subjected to overheating or fire. Full article

This study investigates the seismic retrofit of historic single-nave churches through the optimization of roof diaphragms designed to enhance energy dissipation. The proposed strategy introduces a deformable box-type diaphragm above the existing roof, composed of timber panels and steel connectors with a cover of steel stripes, where energy dissipation is concentrated in the connections. The retrofit design is guided by the estimation of Equivalent Damping Ratio (EDR) instead of the usually adopted resistance criterion, considering an energy-based approach to improve global seismic performance while preserving architectural integrity. In this way, the retrofitted configuration of the roof can be considered a damper. Three numerical phases are presented to assess the effectiveness of the equivalent damping-based intervention. In the first one, the seismic response of the initial non-retrofitted configuration is implemented using a 3D linear finite element model subjected to a response spectrum. Subsequently, nonlinear equivalent models subjected to spectrum-compatible accelerograms are implemented, simulating the possible retrofitted configurations of the roofs to detect the optimum damping and finding the corresponding roof diaphragm configuration. In the third one, the response of the detected retrofitted configuration is also evaluated by nonlinear 3D model subjected to accelerograms. The three phases with the relative numerical approaches are here applied to a case study, located in a high seismic hazard area. The results demonstrate that the EDR-based methodology can optimize the retrofitted roof diaphragm configuration; the nave transverse response is improved in comparison with that designed with the traditional approach, considering only the over-strength of the interventions. Comparisons about the approaches based on the EDR and the strength criteria are presented in terms of lateral displacements, in-plane shear acting on the roof diaphragm, and in-plane stresses on the façade. Full article

Background/Objectives: Financial toxicity (FT) refers to the financial burden directly or indirectly caused by a patient’s medical care. Patients with head and neck cancer (HNC) are particularly vulnerable to FT due to lower rates of return to work and higher out-of-pocket payments (OOPP). In this cross-sectional study, we assessed the amount and types of OOPP, as well as the prevalence of FT, in HNC patients who had completed curative radiotherapy. Methods: We included HNC patients who underwent curative-intent radiotherapy at four private clinics in Romania, within 12 months of completing treatment. Participants completed a 25-item questionnaire capturing sociodemographic information, insurance status, income, and OOPP. To assess subjective FT, we used the validated nine-item Financial Index of Toxicity (FIT), which measures three FT domains: financial stress, financial strain, and lost productivity. Each domain and the total score range from 0 to 100, with higher scores indicating greater financial toxicity. Descriptive statistics were used to summarize patient characteristics. Pearson’s chi-square, t-tests, and one-way ANOVA were used to assess statistical associations, with a significance threshold of p < 0.05. Results: Among 113 patients (mean age: 59), the majority were male (74.3%) and married (74.3%), with 40% having completed university or higher education. The most frequent tumor sites were the oropharynx (29 cases), larynx (22), and oral cavity (21). Concurrent chemoradiation was the most common treatment modality (47%). The mean total FT score was 18.8. Overall, 39.8% of patients experienced financial toxicity, and 29.2% scored above the mean in financial stress. Moderate financial strain (score > 21) was reported by 39.8% of participants, and approximately one-third reported loss of productivity. Transportation and nutritional supplements were the most common OOPP categories. Notably, 42% of patients spent at least 400 euros—equivalent to Romania’s monthly minimum income—on transportation during radiotherapy. FT was significantly associated with employment and marital status, but not with tumor site or treatment type. Conclusions: Among Romanian HNC patients treated with curative radiotherapy, we found substantial OOPP, particularly for transportation and nutritional supplements. While overall FT levels were moderate, divorced patients and those retired due to other chronic conditions were the most vulnerable to financial distress. Financial toxicity can directly affect treatment adherence, survival, and quality of life. By integrating financial counseling, social support, and broader coverage of treatment-related expenses, healthcare systems can mitigate FT for these patients. Full article

The rising incidence of cancer has demanded the development of new anti-cancer chemical sources. The presence of phenolics in hazelnut cell cultures has led to the development of new and potential pharmacotherapeutic uses. Hazelnut extract has emerged as a promising candidate due to its high phytochemical content. HCT-116 colorectal cancer IC₅₀ cell viability of Palaz and Tombul hazelnut extracts was determined as 400 μg/mL and 200 μg/mL, respectively. Flow cytometry annexin V-fluorescein isothiocyante (FITC) apoptosis detection indicated apoptosis of Tombul hazelnut extract and Palaz hazelnut extract as 23.53% and 17.47%, respectively. The apoptosis result of flow cytometry was also supported at the protein level. Hazelnut extracts resulted in an increased loss of MMP as well. The loss of MMP has significantly increased from an average of 0.61% to 16.17% in Tombul hazelnut extract and to 20.38% in Palaz hazelnut extract. This is further supported by screening MICU1, MICU2, PPAR-γ, PPARGC1A, UCP1, UCP2, and UCP3 gene expressions. Targeting apoptosis pathways, particularly MMP, is an effective strategy for cancer prevention and treatment. Hazelnut extract contains phenolic compounds, which activate these pathways, resulting in enhanced apoptosis in colorectal cancer cells. The phenolic contents of Palaz and Tombul hazelnut extracts were determined as 271.72 ± 5.3 mg gallic acid equivalent (GAE)/100 g sample dry weight (DW) and 85.23 ± 2.2 mg GAE/100 g sample DW, respectively. Further, hazelnut extract may reduce oxidative stress, contributing to its anti-cancer properties. The extracts could be utilized as functional ingredients in foods and nutraceuticals to assist with cancer prevention and treatment. Full article

Urban environments, particularly university campuses, are increasingly exposed to thermal discomfort due to the Urban Heat Island (UHI) effect and intense solar radiation. This study evaluates the effectiveness of passive and hybrid cooling strategies, specifically sun-sail shading and mist cooling, in enhancing outdoor thermal comfort (OTC) in a university courtyard. The Van Dyck courtyard at the American University of Beirut, located on the East Mediterranean coast, was selected due to its heavy use between 10 am and 2 pm during summer, when ambient temperatures ranged between 32 and 36 °C and relative humidity between 21 and 33%. Thermal variations across four seating areas were analyzed using ENVI-met, a high-resolution microscale model validated against on-site data, achieving Mean Absolute Percentage Errors of 4% for air temperature and 5.2% for relative humidity. Under baseline conditions, Physiological Equivalent Temperature (PET) exceeded 58 °C, indicating severe thermal stress. Several mitigation strategies were evaluated, including three shading configurations, two mist-cooling setups, and a combined system. Results showed that double-layer shading reduced PET by 17.1 °C, mist cooling by 1.2 °C, and the combined system by 20.7 °C. Shading minimized radiant heat gain, while mist cooling enhanced evaporative cooling, jointly bringing thermal sensations closer to slightly warm–comfortable conditions. These cooling interventions also have sustainability value by reducing dependence on mechanically cooled indoor spaces and lowering campus air-conditioning demand. As passive or low-energy measures, shading and mist cooling support climate-adaptive outdoor design in heat-stressed Mediterranean environments. Full article

This study aimed to evaluate, through finite element analysis (FEA), the biomechanical behavior of edentulous maxillary overdentures supported on 2, 3, and 4 implants with conometric connections, and to determine the minimum implant number that maintains stresses within physiological limits. A 3D finite element model of a resorbed edentulous maxilla was generated from CT images and processed in ANSYS v19.0. Subsequently, six models were simulated according to implant number (2, 3, or 4) and cortical bone thickness (0.5 mm or 1 mm). Conical connection implants and cobalt–chromium-reinforced overdentures with Equator attachments were modeled. Bilateral axial loads were applied and Von Mises equivalent stresses were calculated for implants and abutments, while maximum and minimum principal stresses were analyzed in bone. Results showed that the highest deformation and stress concentrations were observed in the two-implant models, with trabecular stresses ranging from 6.5 to 8.4 MPa, exceeding the 5 MPa safety threshold. In contrast, both three- and four-implant models maintained trabecular stresses below 3 MPa, while keeping cortical bone stresses within physiological limits. The three-implant tripod configuration demonstrated a comparable stress distribution to the four-implant models. From a biomechanical perspective, overdentures supported on four implants with 1 mm cortical thickness showed the most favorable performance. Nevertheless, the three-implant model represented a biomechanically acceptable and potentially cost-effective alternative, suggesting its viability as a simplified clinical option that warrants further investigation. Full article

Thanks to both endogenous and exogenous antioxidants (AOs), the antioxidant defense system ensures redox homeostasis, which is crucial for protecting the body from oxidative stress and maintaining overall health. The food industry also exploits the antioxidant properties to prevent or delay the oxidation of other molecules during processing and storage. There are many classical methods for assessing antioxidant capacity/activity, which are based on mechanisms such as hydrogen atom transfer (HAT), single electron transfer (SET), electron transfer with proton conjugation (HAT/SET mixed mode assays) or the chelation of selected transition metal ions (e.g., Fe²⁺ or Cu¹⁺). The antioxidant capacity (AOxC) index value can be expressed in terms of standard AOs (e.g., Trolox or ascorbic acid) equivalents, enabling different products to be compared. However, there is currently no standardized method for measuring AOxC. Nanoparticle sensors offer a new approach to assessing antioxidant status and can be used to analyze environmental samples, plant extracts, foodstuffs, dietary supplements and clinical samples. This review summarizes the available information on nanoparticle sensors as tools for assessing antioxidant status. Particular attention has been paid to nanoparticles (with a size of less than 100 nm), including silver (AgNPs), gold (AuNPs), cerium oxide (CeONPs) and other metal oxide nanoparticles, as well as nanozymes. Nanozymes belong to an advanced class of nanomaterials that mimic natural enzymes due to their catalytic properties and constitute a novel signal transduction strategy in colorimetric and absorption sensors based on the localized surface plasmon resonance (LSPR) band. Other potential AOxC sensors include quantum dots (QDs, <10 nm), which are particularly useful for the sensitive detection of specific antioxidants (e.g., GSH, AA and baicalein) and can achieve very good limits of detection (LOD). QDs and metallic nanoparticles (MNPs) operate on different principles to evaluate AOxC. MNPs rely on optical changes resulting from LSPR, which are monitored as changes in color or absorbance during synthesis, growth or aggregation. QDs, on the other hand, primarily utilize changes in fluorescence. This review aims to demonstrate that, thanks to its simplicity, speed, small sample volumes and relatively inexpensive instrumentation, nanoparticle-based AOxC assessment is a useful alternative to classical approaches and can be tailored to the desired aim and analytes. Full article

Although the dynamic response of orthotropic materials under uniaxial impact loading has been extensively studied, their behavior under multiaxial stress states, which more accurately represent real-world blast and impact scenarios, has received limited attention. To address this gap, this study employed a self-developed biaxial impact testing apparatus to systematically investigate the dynamic mechanical behavior of beech wood, a typical orthotropic material, under three biaxial loading configurations: radial-tangential, radial-longitudinal, and tangential-longitudinal. By combining theoretical derivation with experimental data, it systematically examines stress wave propagation characteristics, strain rate effects, and anisotropy evolution under different loading paths. The results reveal that beech wood exhibits significantly distinct dynamic responses along different material orientations, with a consistent strength hierarchy: longitudinal > radial > tangential. Biaxial loading notably enhances the equivalent stress–strain response and alters the deformation mechanisms and energy absorption behavior. Furthermore, lateral confinement and multiaxial stress coupling are identified as critical factors influencing the dynamic performance. This study provides the first systematic revelation of the strain rate strengthening mechanisms and wave propagation characteristics of orthotropic materials from the perspective of multiaxial dynamic loading, thereby offering theoretical and experimental foundations for developing advanced dynamic constitutive models suitable for complex impact conditions. These findings provide important guidance for the design and evaluation of lightweight impact-resistant structures in fields such as aerospace and protective engineering. Full article

Background/Objective: Alzheimer’s disease (AD) is a neurodegenerative condition characterized by oxidative stress, neuroinflammation, amyloidogenesis, and tau-related pathology. This study investigated the macronutrient and phytochemical composition of strawberry (S), lemon (L), and lemon juice-assisted strawberry (S/L) extracts and evaluated their neuroprotective efficacy relative to selenium (Se) in an aluminum chloride (AlCl₃)-induced rat model of AD. Methods: Macronutrients and phenolics were quantified in S, L, and S/L, and the extracts were profiled using high-performance liquid chromatography and electrospray ionization tandem mass-spectrometry. Male Sprague–Dawley rats received AlCl₃ with or without S, L, S/L, or Se, and their cognitive performance was assessed using the Morris water maze, Y-maze, and conditioned avoidance tests. Markers of oxidative status, inflammation, cholinergic function, apoptotic signaling, and Wnt3/β-catenin pathway activity were quantified in the brain tissue, and cortico-hippocampal morphology was examined. Results: The S/L extract showed the highest carbohydrate, protein, and lipid content. The total phenolic content was highest in S/L (60.46 mg gallic acid equivalents/g), followed by L (55.08) and S (44.75), with S/L also being the richest in gallic, ellagic, and chlorogenic acids. S/L attenuated AlCl₃-induced cognitive deficits, restored antioxidant status, suppressed neuroinflammation, improved cholinergic indices, modulated apoptotic signaling, and downregulated amyloidogenic and NLRP3 inflammasome markers, consistent with histological evidence of neuronal preservation. Conclusions: Lemon juice-assisted extraction enhanced the macronutrient and phenolic richness and multitarget neuroprotection of strawberries. S/L co-extracts represent promising functional food–derived adjuvants for AD management and support integrative compositional–mechanistic profiling to optimize natural product–based interventions. Full article

With the increasing functional and geometric complexity of modern steel buildings, irregular-shaped beam-to-column joints are becoming common in engineering practice. However, their seismic behavior remains insufficiently understood, particularly for configurations with geometric asymmetry and complex stress transfer mechanisms. This study experimentally investigates the seismic performance of irregular steel-beam-to-concrete-filled steel tube (CFST) column joints incorporating inclined internal diaphragms (IIDs), taking unequal-depth beam (UDB) and staggered beam (SB) joints as representative cases. Two full-scale joint specimens were designed and tested under cyclic loading to evaluate their failure modes, load-bearing capacity, stiffness/strength degradation, energy dissipation capacity, strain distribution, and panel zone shear behavior. Both joints exhibited satisfactory strength and initial stiffness. Although diaphragm fracture occurred at approximately 3% drift, the joints retained 45–60% of their peak load capacity, based on the average strength of several loading cycles at the same drift level after diaphragm failure, and maintained stable hysteresis with average equivalent damping ratios above 0.20. Final failure was governed by successive diaphragm fracture followed by the tearing of the column wall, indicating that the adopted diaphragm thickness (equal to the beam flange thickness) was insufficient and that welding quality significantly affected joint performance. Refined finite element (FE) models were developed and validated against the test responses, reasonably capturing global strength, initial stiffness, and the stress concentration patterns prior to diaphragm fracture. The findings of this study provide a useful reference for the seismic design and further development of internal-diaphragm irregular steel-beam-to-CFST column joints. Full article

Background: The intensification of aquaculture has led to increased use of chemical agents, such as trichlorfon, for controlling parasitic infections in farmed fish. While effective, this organophosphate compound may exert toxic effects even at sublethal concentrations, posing risks to economically important species such as tambaqui (C. macropomum). This study investigated the molecular effects of trichlorfon on the expression of genes involved in stress response, energy metabolism, and apoptosis in juvenile tambaqui. Methods: Fish were exposed to two sublethal concentrations of trichlorfon (30% and 50% LC_{50–96 h}, equivalent to 0.261 and 0.435 mg/L) for 48, 72, and 96 h. Expression levels of fkbp5, p53, pim-2, pir, me1, bbox1, and higd1a were quantified in liver tissue using qPCR. Results: fkbp5 and p53 were strongly upregulated at 48 h, indicating acute stress and genotoxic activation. me1 and pim-2 were also upregulated, reflecting activation of compensatory energy metabolism and anti-apoptotic survival pathways. bbox1 showed an early induction followed by collapse at 96 h, while higd1a and pir exhibited delayed overexpression at 96 h, suggesting mitochondrial hypoxia and inflammation. Conclusions: Trichlorfon triggers a multifaceted toxic response characterized by initial activation of compensatory pathways (stress response, antioxidant defense, and anti-apoptotic mechanisms) followed by late-phase metabolic collapse, mitochondrial hypoxia, and inflammation, with both time- and dose-dependent effects. These findings demonstrate that even sublethal concentrations disrupt hepatic homeostasis and support the use of these genes as molecular biomarkers for environmental monitoring in aquaculture. Full article