Search Results (21)

Lingonberry pomace (LP) is a by-product rich in valuable bioactive compounds and can be used in the food industry after various treatments and property characterization. This study aimed to evaluate the impact of commercially available enzymes (Viscozyme^® L, Pectinex^® Ultra Tropical, and Celluclast^® 1.5 L) and supercritical carbon dioxide (SFE-CO₂) extraction technology on the chemical composition and technological properties of treated LP products. The Megazyme kit was used to determine the soluble dietary fiber (SDS) and insoluble dietary fiber (IDF) contents, while the changes in mono-, disaccharide, and oligosaccharides were analyzed by applying high-pressure liquid chromatography with a refractive index detector. The analyzed properties were as follows: the water swelling capacity (WSC), water retention capacity (WRC), water solubility index (WSI), oil retention capacity (ORC), bulk density (BD), and emulsion stability of modified LP. The tested LP contained 8.49 g/100 g of SDF and 65.36 g/100 g of IDF (in dry matter). The partial separation of lipophilic substances during SFE-CO₂ extraction did not significantly affect the enzymatic hydrolysis efficiency. The amount of oligosaccharides in the LP increased using enzymes with pectinolytic activity (Viscozyme^® L and Pectinex^® Ultra Tropical), while cellulolytic enzymes (Celluclast^® 1.5 L) increased the amount of SDF and improved the IDF/SDF ratio. Enzymatic hydrolysis increased the SI, WRC, and ORC of LP powder. Emulsions with LP hydrolyzed with Pectinex^® Ultra Tropical demonstrated the highest stability during storage. This study demonstrates that the modification of LP powders provides diverse technological properties, which could expand the application of such products for further food production. Full article

Background/Objectives: Chitosan–alginate microcapsules were produced to encapsulate bioactive compounds from Artemisia annua L. extract (apigenin, luteolin) and cannabidiol (CBD). The study aimed to optimize emulsion composition and encapsulation parameters for potential applications in food supplements and pharmaceuticals. Methods: A water-in-oil-in-water (W/O/W) emulsion and a modified coacervation extrusion technique were employed. The study was conducted in two phases using response surface methodology. Key metrics included encapsulation efficiency (EE), yield (EY), cumulative release in vitro, and physicochemical and morphological properties, analyzed via scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), high-performance liquid chromatography with a diode array detector (HPLC-DAD), and gas chromatography with flame ionization detection (GC-FID). Results: The optimal conditions were identified as 0.1% Tween 20, 3.8% Span 80, 3.8% CBD, 19.9% A. annua L. extract, 1.5% outer-phase Tween 20, 48.5% sodium alginate, 200 rpm stirring for 30 min, and a 0.05 mL/min flow rate. The EE values were 80.32 ± 4.11% for CBD, 88.13 ± 3.13% for apigenin, and 88.41 ± 4.17% for luteolin, with respective cumulative releases of 77.18 ± 4.4%, 75.12 ± 4.81%, and 75.32 ± 4.53%. Conclusions: The developed microcapsules demonstrated high encapsulation efficiency and controlled release, highlighting their potential for further development in food supplements and pharmaceuticals. Future studies should focus on refining the formulation for improved bioavailability and stability. Full article

In high-altitude experiments to study the central cores of EAS at E₀ ≳ 10¹⁶ eV (√s ≳ 5 TeV) using X-ray emulsion chambers and ionization calorimeters, phenomena such as the coplanarity of the arrival of the most energetic particles in super families of γ-rays and hadrons and a so-called Tien Shan effect (too slow absorption of cascades initiated by high-energy hadrons in the calorimeter) were observed. These effects could not be reproduced within the framework of theoretical models of the 80s and 90s. The coplanarity is explained via a process of coplanar generation of the most energetic secondary particles in interactions of super high-energy hadrons with nuclei of air atoms. Perhaps the Tien Shan effect could be explained using a high cross section for the generation of fragmentation-region charmed hadrons. To study these phenomena, a new set of detectors has been developed, including the world’s highest high-mountain ionization calorimeter, “Hadron-55”. This paper presents the initial experimental results. Full article

The growing consumer demand for the development of functional foods with a number of benefits for the consumer has led to a considerable increase in the studies focused on examining different natural agents to be included in the composition of newly developed functional foods. The chemical compositions of the essential oils (EOs) of lavender (Lavandula angustifolia Mill.), peppermint (Mentha piperita L.), raspberry seed (Rubus idaeus L.), and ylang-ylang (Cananga odorata (Lam.)) were determined using gas chromatography with a mass selective detector (GC-MS). The antibacterial and antifungal activities of these EOs were examined using a high-throughput 96-well microplate bioassay procedure and the MICs of each EO against each test microorganism were determined. The results indicated significant antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Salmonella abony NTCC 6017, Pseudomonas aeruginosa NBIMCC 1390, Bacillus subtilis ATCC 19659, Penicillium chrysogenum ATCC 28089, Fusarium moniliforme ATCC 38932, Aspergillus niger ATCC 1015, and Aspergillus flavus ATCC 9643. To explore their potential applications in food preservation, model chocolate mousse food emulsions were prepared that incorporated EOs and/or selected probiotic lactobacilli strains in both free and encapsulated forms. The inclusion of EOs and/or probiotic lactobacilli resulted in enhanced microbial safety and an extended shelf life. Furthermore, the chocolate mousse variants that were biopreserved with the inclusion of probiotic lactobacilli maintained a high viable lactobacillus cell concentration throughout the storage period. As a result, these products would not only be suitable as functional probiotic foods but also as effective delivery vehicles for probiotic lactobacilli. Full article

SND@LHC is designed to perform measurements with neutrinos produced at the LHC in the pseudo-rapidity range of $7.2<\eta <8.4$. The experiment is located 480 m downstream of the ATLAS interaction point in the TI18 tunnel. The detector is a hybrid system composed of an 830 kg target made from 1 mm thick tungsten plates interleaved with nuclear emulsion films, electronic trackers also acting as an electromagnetic calorimeter, a hadronic calorimeter and a muon identification system. The detector is able to distinguish three neutrino flavours using the emulsion detector which can identify primary electrons and taus in charged current neutrino interactions. This capability allows probing heavy flavour forward production at the LHC, which even LHCb cannot access. The LHC CM energy corresponds to the ${10}^{17}$ eV astronomical energy region, which is of interest for future detectors. The SND@LHC’s capabilities and current status are reported in this document. Full article

The SND@LHC detector experiment is located at the Large Hadron Collider (LHC), about 480 m downstream of the ATLAS interaction point. The detector is designed to measure, for the first time ever, high-energy neutrinos produced at the LHC in the pseudorapidity region of $7.2<\eta <8.4$, which is inaccessible to other LHC experiments. The detector comprises a hybrid system that incorporates multiple components. The detector includes a 830 kg target composed of tungsten plates arranged in alternating layers with nuclear emulsion and electronic trackers: this arrangement functions as an electromagnetic calorimeter. Following the electromagnetic calorimeter, there is a hadronic calorimeter and a muon identification system. The detector possesses the ability to differentiate interactions involving all three neutrino flavours, enabling investigations into the physics of heavy flavour production in the forward region. This research is particularly significant for future circular colliders and high-energy astrophysical neutrino experiments. Furthermore, the detector has the ability to search for the scattering of Feebly Interacting Particles. The detector started operating during the LHC Run 3, and it collected a total of ∼39 fb${}^{-1}$ in 2022. The detector aims to collect approximately 250 fb${}^{-1}$ in the whole of Run 3. Full article

SND@LHC (Scattering Neutrino Detector at the Large Hadron Collider) is a compact and stand-alone experiment to perform measurements with neutrinos produced in the LHC in a hitherto unexplored pseudorapidity region of 7.2 < η < 8.6. The experiment is located in the Tl18 (Target line 18) LHC tunnel, 480 m downstream of the ATLAS detector interaction point. The SND@LHC detector is composed of a hybrid system based on an 800 kg target mass of tungsten plates, interleaved with emulsion and electronic trackers, followed downstream by a muon system. This configuration allows us to distinguish all three neutrino flavors, opening a unique opportunity to probe the physics of heavy flavor production in the LHC in a region that is not accessible to the ATLAS, CMS, LHCb and FASER experiments. The detector concept is also well suited to searching for feebly interacting particles via signatures of scattering in the detector target. The first phase of the experiment has been carried out during the ongoing LHC Run 3, and the first data of the LHC Run3 commissioning period are being processed and analyzed. Full article

Herein, we report results of the studies relating to the development of an impedimetric, magnetic bead-assisted supersandwich DNA hybridization assay for ultrasensitive detection of Neisseria gonorrhoeae, the causative agent of a sexually transmitted infection (STI), gonorrhea. First, a conductive ink was formulated by homogenously dispersing carboxylated multiwalled carbon nanotubes (cMWCNTs) in a stable emulsion of terpineol and an aqueous suspension of carboxymethyl cellulose (CMC). The ink, labeled C5, was coated onto paper substrates to fabricate C5@paper conductive electrodes. Thereafter, a magnetic bead (MB)-assisted supersandwich DNA hybridization assay was optimized against the porA pseudogene of N. gonorrhoeae. For this purpose, a pair of specific 5′ aminated capture probes (SCP) and supersandwich detector probes (SDP) was designed, which allowed the enrichment of target gonorrheal DNA sequence from a milieu of substances. The SD probe was designed such that instead of 1:1 binding, it allowed the binding of more than one T strand, leading to a ‘ladder-like’ DNA supersandwich structure. The MB-assisted supersandwich assay was integrated into the C5@paper electrodes for electrochemical analysis. The C5@paper electrodes were found to be highly conductive by a four-probe conductivity method (maximum conductivity of 10.1 S·cm⁻¹). Further, the biosensing assay displayed a wide linear range of 100 aM-100 nM (10⁹ orders of magnitude) with an excellent sensitivity of 22.6 kΩ·(log[concentration])⁻¹. The clinical applicability of the biosensing assay was assessed by detecting genomic DNA extracted from N. gonorrhoeae in the presence of DNA from different non-gonorrheal bacterial species. In conclusion, this study demonstrates a highly sensitive, cost-effective, and label-free paper-based device for STI diagnostics. The ink formulation prepared for the study was found to be highly thixotropic, which indicates that the paper electrodes can be screen-printed in a reproducible and scalable manner. Full article

Mayonnaise is an oil-in-water emulsion containing 70–80% finely dispersed droplets of oil in a continuous phase of water. Since mayonnaise has a sour and acidic taste, its sugar profile is barely noticed and thus often disregarded. However, today, there are various variants of mayonnaise available on the market; hence, it is crucial to understand their mono- and disaccharide profile, in order to determine the precise total sugar composition. The traditional methods of sugar analysis available, such as titration, can only quantify sucrose and are unable to differentiate between mono- and disaccharides. The aim of this study was to develop and validate a method for the quantification of total sugars, including fructose, glucose, sucrose, and lactose, in eggless mayonnaise, using a high performance liquid chromatography refractive index detector (HPLC-RID). Sugars were separated on an amino column with an oven temperature of 35 °C, using an isocratic solvent system consisting of a 75:25 v/v mixture of acetonitrile and HPLC water, at a 0.9 mL/min flow rate with RID. Method validation was performed for the linearity, specificity, precision, accuracy, LOD, LOQ, and robustness. A linearity for total sugars, with a regression coefficient of 0.9998, was obtained within the range of 0.05024 to 10.048 mg/mL. The relative standard deviation was less than 2.0% for the intra-day and inter-day precision. The accuracy was found to be 96.78–108.88% using a three-level recovery method. The LOD and LOQ were also found to be suitable. The samples used in this study contained 0.24–10.32% total sugars. The sucrose value obtained matched the label claim of the products and no significant differences were observed between results in a paired sample t-test. This showed the applicability of the proposed method for analyzing the sugar profile in a finished product. Routine analysis of total sugars in eggless mayonnaise and similar finished products can thus be performed using this technique, which was found to be simple, rapid, and reproducible. Full article

In this work, two bio-based raw materials, rapeseed oil and technical-grade oleic acid, were modified to yield acrylated monomers. Polymeric latexes designed for coating applications were synthesized using emulsion polymerization. Methyl methacrylate and butyl acrylate were copolymerized with various ratios of the acrylated bio-based monomers (0–20 wt.% in the monomer mixture). The polymerization with high monomer conversion and low coagulum content was successfully performed up to the content of 15 wt.% of the bio-based monomers, leading to long-term stable latexes. The asymmetric flow field flow fractionation coupled with a multi-angle light scattering detector was used to describe the molar mass distribution of the synthesized copolymers. Ultra-high molar mass fractions were detected in copolymers comprising the bio-based monomers due to the presence of multi-acrylated bio-based ingredients originating from linoleic and linolenic fatty acids. This phenomenon became more pronounced for the rapeseed oil-originated monomer. The prepared latexes comprising copolymerized bio-based monomers showed comparable or even better coating performance in terms of gloss and water resistance in comparison with the reference acrylic coating, which makes the acrylated vegetable oil-based monomers attractive for the manufacturing of sustainable water-borne materials in the coating industry. Full article

In the current cosmetic and personal care industry, it is of great importance to have a technique that detects instabilities quickly and effectively, as consumers are demanding more innovative and sustainable ingredients. Diffusing wave spectroscopy (DWS) is a potential solution as it is a modern optical technique that can measure the spatial movement of particles or droplets in an emulsion—i.e., the mean square displacement (MSD), as a function of time. In the current investigation, systematic visual and diffusion behavior emulsion stability studies over a 3-h period on jojoba and avocado oil emulsions containing varying equal percentages of cocamidopropyl betaine (CAPB) and sodium lauryl ether sulfate (SLES) were conducted. The turbid emulsions studied had differing stabilities with unknown instability mechanisms to further explore if diffusing wave spectroscopy can offer a fast and early identification of problem cosmetic formulations. It was observed that, for emulsions displaying instability from 4 to 123 h, the greater the change in the MSD values over a 3-h period, the greater the instability of the emulsion. For all systems, the MSD values lowered and shifted to the right from hour 0 to hour 3. We conjecture that the emulsion droplets began to aggregate, potentially growing and giving rise to larger particles. The increasing particle size was the cause for the slowing down of the dynamics and thus diffusion, giving rise to the lowering of the MSD values. Our findings indicate that by testing an emulsion over a 3-h period, it is possible to determine whether it will be a problem formulation using DWS. Studies into this technology should be continued on a wider range of emulsions with known instability mechanisms to further our understanding of using DWS as a vital emulsion instability detector. Full article

This work aimed to research the efficiency of gamma irradiation and shielding characteristics on the lead oxide (PbO) doped the crosslinked polystyrene-b-polyethyleneglycol (PS-b-PEG) block copolymers and polystyrene-b-polyethyleneglycol-boron nitride (PS-b-PEG-BN) nanocomposites materials. The crosslinked PS-b-PEG block copolymers and PS-b-PEG-BN nanocomposites mixed with different percentage rates of PbO were used to research gamma-ray shielding characteristics. The synthesis of the copolymer was done by emulsion polymerization methods. The characterization and morphological analyses of irradiated samples were explored handling with the Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FTIR), Gel Permeation Chromatography (GPC), Thermogravimetric Analysis (TGA), and Scanning Electron Microscope (SEM) methods. The gamma-rays that were emitted from the $E_{ }^{152}u$ source were observed with a High Purity Germanium (HPGe) detector system and examined with a GammaVision computer program. Our samples, including the different percentage rates of the PS-b-PEG (1000, 1500, 10,000), BN, and PbO, were irradiated in various gamma-ray photon energy regions (from 121.78 keV to 1408.01 keV). Then, Linear-Mass Attenuation Coefficients (LACs-MACs), Half-Tenth Value Layer (HVL), Mean Free Path (MFP), and Radiation Protection Efficiency (RPE) values of the samples were calculated. Via crosschecking the acquired data from samples with and without PbO and BN, it was observed that, if the different percentage rates by weight nano-powder of PbO and BN are added in the polymer mixture, it can be used as a convenient shielding material against gamma rays. Full article

A neutron detector using a fine-grained nuclear emulsion has a sub-micron spatial resolution and thus has potential to be applied as high-resolution neutron imaging. In this paper, we present two approaches to applying the emulsion detectors for neutron imaging. One is using a track analysis to derive the reaction points for high resolution. From an image obtained with a 9 μm pitch Gd grating with cold neutrons, periodic peak with a standard deviation of $1.3$ μm was observed. The other is an approach without a track analysis for high-density irradiation. An internal structure of a crystal oscillator chip, with a scale of approximately 30 μm, was able to be observed after an image analysis. Full article

Fat-spread products are a stabilized emulsion of water and vegetable oils. The whole fat content can vary from 10 to 90% (w/w). There are different kinds, which are differently named, and their composition depends on the country in which they are produced or marketed. Thus, having analytical solutions to determine geographical origin is required. In this study, some multivariate classification methods are developed and optimised to differentiate fat-spread-related products from different geographical origins (Spain and Morocco), using as an analytical informative signal the instrumental fingerprints, acquired by liquid chromatography coupled with a diode array detector (HPLC-DAD) in both normal and reverse phase modes. No sample treatment was applied, and, prior to chromatographic analysis, only the samples were dissolved in n‑hexane. Soft independent modelling of class analogy (SIMCA) and partial least squares-discriminant analysis (PLS-DA) were used as classification methods. In addition, several classification strategies were applied, and performance of the classifications was evaluated applying proper classification metrics. Finally, 100% of samples were correctly classified applying PLS-DA with data collected in reverse phase. Full article

The present work investigated the efficacy of Moringa flower (MF) extract to develop a functional chicken product. Three groups of cooked chicken nuggets—control (C), T1 (with 1% MF) and T2 (2% MF)—were elaborated and their physicochemical, nutritional, storage stability and sensory attributes were assessed during refrigerated storage at 4 °C up to 20 days. In addition, MF extracts were characterised in terms of chemical composition, total phenolic content and its components using high-performance liquid chromatography with a diode-array detector (HPLC-DAD), dietary fibre and antioxidant capacity. MF contained high protein (17.87 ± 0.28 dry matter), dietary fibre (36.14 ± 0.77 dry matter) and total phenolics (18.34 ± 1.16 to 19.49 ± 1.35 mg gallic acid equivalent (GAE)/g dry matter) content. The treated nuggets (T1 and T2) had significantly enhanced cooking yield, emulsion stability, ash, protein, total phenolics and dietary fibre compared to control. Incorporation of MF extract at 2% not only significantly reduced the redness/increased the lightness, but also decreased the hardness, gumminess and chewiness of the product compared to control. Moreover, the addition of MF extract significantly improved the oxidative stability and odour scores by reducing lipid oxidation during storage time. Sensory attributes of nuggets were not affected by the addition of MF extract and the products remained stable and acceptable even on 15th day of storage. These results showed that MF extract could be considered as an effective natural functional ingredient for quality improvement and reducing lipid oxidation in cooked chicken nuggets. Full article