Search Results (41,165)

The brewing of Traditional Hakka Huangjiu (THHJ) is usually divided into saccharification and post-fermentation. Microbial succession during saccharification is the major factor influencing the development of the volatile and non-volatile substances in THHJ during post-fermentation. This study systematically investigated the dynamic changes in microbial community, volatile substances and microbial metabolites by using absolute quantitative sequencing and multi-omics analysis. This study also reported that the correlation between microorganisms and substance biosynthesis was analyzed using PICRUSt. Absolute quantitative sequencing results showed that Pediococcus, Saccharomycopsis, Rhizopus, Weissella, and Limosilactobacillus were the dominant microbial genera during saccharification. 737 volatile compounds (170 esters, 94 hydrocarbons, 82 organoheterocyclic compounds) and 4370 metabolites (18 organic acids, 22 amino acids, 1124 peptides and 9 categories of functional compounds) were identified throughout the post-fermentation period. Sensory profiling revealed six main flavor attributes (Balsamic, sweet, rose, green, fruity, bitter) in THHJ and phenylethyl alcohol exerted the most prominent effect on the overall flavor of THHJ. Correlation analysis revealed that the biosynthesis of phenylethyl alcohol was potentially correlated with Saccharomyces, Cyberlindnera, Pichia, Pediococcus, Pseudomonas and Lactococcus. The biosynthesis of flavonoids was potentially correlated with Pediococcus, Lactococcus, and Lactiplantibacillus. These findings contribute to monitoring product quality and optimizing the processing techniques of THHJ. Full article

Xinjiang is the major cotton-producing region in China, and identifying core germplasm with disease resistance, high yield, and high seed index is of great significance for guiding local cotton production and breeding practices. Using 182 upland cotton germplasm accessions, we systematically investigated Verticillium wilt (caused by Verticillium dahliae) disease index, yield, and seed index in Kuitun, Xinjiang, during 2018–2019. Comparative analysis revealed that the germplasm from the Yellow River Ecological Region (YER) exhibited the strongest disease resistance but ranked second in yield, while the germplasm from the Northwest Inland Ecological Region (NWC) was susceptible to disease, yet had the highest yield, indicating great potential for further improving cotton yield in Kuitun. The Verticillium wilt index decreased, and yield increased with breeding periods. PCA and K-Means clustering divided germplasm into three clusters, with Cluster 0 being disease-resistant, high-yielding, and having a high seed index. Using the 20th percentile method, 20 core germplasm (11.0% of total) were selected, including disease-resistant and high-yield accessions, 3 disease-susceptible and high-yield accessions, and 6 disease-resistant and high seed index accessions. The results of this study provide important material support and a theoretical basis for the synergistic breeding of cotton with disease resistance, high yield, and high seed index in Xinjiang. Full article

This study proposes a method to quantify uncertainty in the scattering parameter (S-parameter) measurements when using de-embedding techniques. After calibrating the measurement setup with reference standards, de-embedding algorithms are employed to extract the intrinsic S-parameter of the device under test (DUT). This process introduces additional complexity to the uncertainty analysis. This study investigates the sources of uncertainty inherent to vector network analyzer (VNA) measurements. Subsequently, a covariance matrix-based approach is employed to propagate these uncertainties, culminating in the quantification of S-parameter uncertainty. The effectiveness of the proposed is determined by comparing the measured S-parameters of power dividers and couplers to their nominal values, considering parameters such as balance, coupling, and voltage standing wave ratio (VSWR). Additionally, an uncertainty analysis is conducted for the power divider’s S-parameters, tracing the uncertainty sources back to the calibration standards. Full article

In many densely populated towns and semi-urban areas, masonry buildings often stand close to busy roads, exposing them to blasts from improvised explosives or other localized sources. Such structures are rarely designed to resist sudden explosive forces, making severe damage or even progressive collapse likely. Even moderate-intensity blasts can weaken walls, endanger occupants, and cause significant property loss. Unlike reinforced concrete, masonry is highly susceptible to explosive impact. Therefore, understanding how these buildings behave under blast loads and developing affordable protection methods is crucial. Low-rise unreinforced masonry (URM) structures, usually up to about 13 m in height (roughly 2–4 stories), common in villages, semi-urban regions, and conflict-prone zones, are particularly at risk. In many areas, these poorly constructed buildings lack proper engineering design and are therefore highly vulnerable to blast damage. Non-load-bearing internal dividers and perimeter enclosures are especially prone to lateral displacement, which can initiate instability and, in severe cases, lead to overall structural failure. This research focuses on reducing catastrophic damage in URM walls when exposed to close-proximity blast forces using concrete-based protective coatings, both with and without embedded steel-welded wire mesh. The study references a previously tested laterally supported clay brick wall built with cement–sand mortar as the baseline model, with its behavior validated against experimental findings from existing literature. Two blast cases were considered corresponding to scaled stand-off distances of 2.19 m/kg^1/3 and 1.83 m/kg^1/3, representing moderate flexural-shear cracking and full structural failure, respectively. To replicate the observed behavior, a comprehensive 3D numerical simulation was developed using the ABAQUS/Explicit 2020 solver. The model’s predictions were benchmarked and verified through comparison with reported test data. While both blast intensities were used to confirm computational accuracy, the effectiveness of UHPC and UHPFRC protective coatings with and without embedded wire mesh was specifically evaluated under the more severe collapse scenario (Z = 1.83 m/kg^1/3). Results indicated that at a scaled distance of 1.83 m/kg^1/3, the uncoated URM wall could not withstand the blast because of poor tensile and bending capacity. In contrast, the UHPC- and UHPFRC-coatings provided improved confinement and better stress distribution. When welded wire mesh was embedded, crack control improved further, the interface bond strengthened, and a larger portion of blast energy was absorbed and dissipated. Full article

(1) Background: This experiment investigated the effects of balancing methionine or lysine under different dietary protein levels on intestinal function and immune organ development in Wanxi white geese during the brooding period. (2) Methods: A total of 180 one-day-old Wanxi white geese were randomly divided into six groups using a 2 × 3 factorial design, with amino acid balance (lysine or methionine) and dietary crude protein levels (20%, 18%, and 16%) as experimental factors. Each group consisted of five replicates with six geese per replicate. The feeding trial lasted 28 days. (3) Results: The lysine-balanced diet significantly increased jejunal lipase activity in 14-day-old geese (p < 0.05). In contrast, the methionine-balanced diet significantly enhanced ileal trypsin, maltase, and amylase activities at 14 days, as well as ileal trypsin activity at 28 days (p < 0.05). Lysine balance markedly improved jejunal weight and muscularis thickness, ileal weight, villus height, and villus-to-crypt (V/C) ratio in 14-day-old geese, and further increased jejunal villus height and ileal crypt depth and muscularis thickness at 28 days (p < 0.05). Methionine balance significantly elevated total antioxidant capacity (T-AOC) in the jejunum and ileum of geese at both 14 and 28 days (p < 0.05). Moreover, the methionine-balanced group exhibited a significantly higher thymus index and spleen index in 28-day-old geese compared with the lysine-balanced group (p < 0.05). Notably, geese fed the 18% crude protein diet showed significantly greater follicular and medullary areas of the bursa of Fabricius at 14 days than those fed 20% or 16% protein diets (p < 0.05). (4) Conclusions: Appropriate dietary protein reduction combined with balanced lysine or methionine supplementation effectively enhances intestinal digestive function, antioxidant capacity, and immune organ development in Wanxi white geese. Among the tested treatments, a dietary protein level of 18% produced the most favorable overall outcomes during the brooding period. Full article

Background/Objectives: Although anti-Müllerian hormone (AMH) is a strong biomarker of ovarian reserve and oocyte pools, it is unknown whether high AMH levels can be a reliable predictor of oocyte quality, ovulation, and embryo quality. We aimed to determine whether there is any AMH threshold value that can be used to predict treatment success in women with clomiphene citrate (CC) resistance or failure in polycystic ovary syndrome (PCOS). Methods: This retrospective cohort study included 93 infertile women with PCOS who had been previously diagnosed with CC failure or CC resistance between May 2017 and June 2018. Prior to treatment, AMH concentration was measured in all women. The participants were divided into 2 groups according to their conception after ovulation induction (OI) and intrauterine insemination (IUI). At the end of a one-year period, the medical files were assessed retrospectively. Those with and without pregnancy were compared in terms of treatment protocols, infertility periods, laboratory parameters and AMH levels. Results: Clinical and biochemical characteristics of 36 pregnant women were compared with those of 57 non-pregnant women. The results showed that the pregnant group had significantly shorter infertility periods and longer ovarian stimulations than the non-pregnant group (p < 0.05). Serum AMH levels > 4.5 ng/mL can predict OI and IUI outcome in this specific patient population, with a sensitivity of 56% and a specificity of 69%. Multivariate logistic regression analysis showed that only AMH was identified as an independent predictor of pregnancy [OR = 1.151 (95% CI: 1.034–1.280), p = 0.010]. Conclusions: Serum AMH may serve as an adjunct predictor of OI and IUI outcomes in infertile women with PCOS who failed to conceive after ≥3 cycles of CC. However, its predictive value appears to be context-dependent and should be interpreted cautiously in clinical practice. Given the distinct clinical characteristics of this patient population, individualized treatment strategies and consideration of earlier alternative therapeutic approaches may be warranted to optimize reproductive outcomes. Full article

This study presents an experimental investigation into the rehabilitation of fire-damaged reinforced concrete (RC) columns using Ultra-High-Performance Concrete (UHPC) under an eccentric load of (e = 45 mm). The experimental program comprised nine small-scale RC column specimens, which were divided into two groups based on exposure temperatures of 500 °C and 700 °C, applied using a specially designed furnace. A control column that was not exposed to fire was also tested for comparison. The study included two fire exposure durations: 60 and 120 min. During the heating phase, the columns were subjected to a pre-applied axial load equal to 50% of their ultimate capacity (Pu). After sustaining fire-induced damage, the columns were rehabilitated using UHPC jacketing. The experimental results revealed a reduction in the ultimate load-carrying capacity of the RC columns with increasing fire temperature and exposure duration. Specifically, the load capacity decreased by 22.68% and 33.89% when exposed to 500 °C for 60 and 120 min, respectively, and by 42.02% and 49.02% when exposed to 700 °C for 60 and 120 min, respectively, compared with the control column. However, strengthening the fire-damaged columns with UHPC significantly enhanced their structural performance, resulting in an increase in ultimate load capacity ranging from 81.88% to 157.14% compared with their corresponding fire-damaged unstrengthened specimens. Based on the experimental findings, the load lateral displacement response at mid-height, load–axial deformation curves, failure modes, ductility, and stiffness characteristics of the columns were analysed. The study concludes that the use of UHPC in rehabilitating fire-exposed columns substantially improves most of these structural properties. Full article

The integrity protection of digital signals is an important task in modern applications. We propose DIPS (Data Integrity Protection of Signals), a fragile watermarking algorithm aiming to protect the integrity of sampled signals like images composed of pixels or sampled audio signals that can be divided into block units. The present paper starts with two works that propose fragile watermarking algorithms yielding high-quality watermarked objects, identifies their security vulnerabilities, and finally defines a method that embeds a compressed Message Authentication Code of each block into the LSBs of the block samples. As it modifies 2 bits per block at most, the introduced distortion is extremely low, thus resulting in a very high objective quality ($PSNR$). Experimental results confirming this characteristic are reported on real sampled signals such as speech, images, and ECG signals. Full article

Endometrial cancer (EC) is the leading gynecological malignancy worldwide. Obesity is reported to be associated with 50% of EC cases. Significant gaps remain in investigating specific molecular mechanisms behind the obesity-driven EC. Women diagnosed with EC undergoing total hysterectomy were recruited. Patients were divided into two groups: EC-obese with BMI > 29.9 kg/m² (n = 10) and EC-Non-obese with BMI ≤ 29.9 kg/m² (n = 10). Tumor tissues were subjected to label-free quantitative proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differentially expressed proteins were identified and subjected to pathway enrichment and network analyses to characterize obesity-associated alterations. Proteomic profiling showed a significant dysregulation of 456 proteins: 171 upregulated and 285 downregulated. Proteins involved in endoplasmic reticulum quality control particularly endoplasmic reticulum lectin 1 (ERLEC1), were reduced. Conversely, EC-obese demonstrated upregulation of hepatocyte growth factor (HGF), integrin-linked kinase (ILK), CTTNBP2 N-terminal-like protein (CTTNBP2NL), and lysyl oxidase homolog 1 (LOXL1), implicating activation of inflammatory pathways. Bioinformatic analysis showed downregulation of immune-related pathways, including neutrophil degranulation, complement activation in the EC-obese group. ROC analysis identified apolipoprotein(a), phospholipase B-like 1, CTTNBP2NL, and ILK as significant proteins that can differentiate between the obese and non-obese states. Our findings provide insights into obesity-associated proteomic changes in EC and highlight candidate proteins that can be used for molecular stratification after further validation. Full article

Background: This retrospective study assessed long-term survival outcomes of severely periodontally compromised mandibular incisors (≥50% bone loss) following initial periodontal treatment and a structured recall protocol. Methods: Ninety-three patients with ≥50% bone loss in all mandibular incisors were treated in a private practice over a 32-year period by the same periodontist. Following initial treatment, patients were assigned 6- or 12-month recall intervals based on response and motivation. The baseline was set after subgingival debridement (visit 3). Last follow-up visit (LFV) in this study was defined as follows: the last control visit of the patients done by the periodontist. ‘Survival’ was divided into 3 groups: complete survival (CS), all incisors were still present, and partial survival (PS), one or two incisor(s) were lost. Total failure (TF) involved instances in which all incisors were lost. Effective survival was monitored when an extracted tooth was repositioned and stabilized with a splint, ensuring preservation of function. Only 9.7% of patients needed a mandibular incisal splint. For reasons of consistency the CPITN was used. Statistical analysis was performed in R. The significance level was set at α = 0.05. Event-free patients can be considered as uninformative censoring, all with the same probability of risk, as they all were still in follow-up at the time of informed consent approval. Results: A total of 93 patients were included in the study. The mean follow-up was 17.7 years. At the last visit, 79.6% of patients retained all incisors, with an effective survival rate of 89.2%. Regarding the survival probability over time, after 15 years, it is 91% (95% CI: 0.86–0.98), and after 20 years, it is 78% (95% CI: 0.69–0.90). The effective survival probability over time after 15 years was 95% (95% CI: 0.91–1.0), and after 20 years, it was 89% (95% CI: 0.81–0.98). Compliance significantly influenced survival (p = 0.007), whereas the number of occluding units did not (p = 0.226). The total amount of teeth lost during the entire follow-up period showed a statistically significant difference compared to survival (p < 0.001). The general periodontal health of the patient population presented a shift from CPITN 3 to the 0–2 group. Conclusions: Severely compromised mandibular incisors demonstrate high long-term survival rates with appropriate therapy. After 20 years the survival probability was 78%, and the effective survival probability, 89%, underscoring the critical role of lifelong periodontal care. Mandibular incisor preservation over long-term follow-up is highly achievable. Full article

Autoimmune thyroid diseases (AITD) are based on reactivity to thyroid self-antigens, resulting in varying degrees of persistent inflammation and glandular hyperplasia. The aim of this study was to investigate the interplay between angiogenesis, inflammation, and oxidative stress in patients with AITD. The study included patients with AITD, divided into a group with Hashimoto’s thyroiditis (HT) and a group with Graves’ disease (GD), as well as healthy controls. The results showed that subjects with GD had significantly higher concentrations of angiopoietin-2 (Ang-2) compared to those with HT and the healthy controls (p < 0.001). Inflammatory parameters (C-reactive protein (CRP), the systemic inflammatory immune response index (SII), and the CRP/albumin ratio (CRP/alb)) were higher in both AITD groups (p < 0.001). Oxidative stress parameters were more pronounced in AITD, while the activity of antioxidant enzymes was reduced. Ang-2 positively correlated with H₂O₂ (r = 0.394, p = 0.006) and NO (r = 0.519, p = 0.001) in HT, as well as with O₂⁻ (r = 0.232, p = 0.009) and TBARS (r = 0.190, p = 0.038) in GD, while in GD it showed a negative correlation with SOD (r = −0.426, p = 0.012) and CAT (r = −0.534, p = 0.008). Thus, angiogenesis, inflammation, and oxidative stress are interconnected processes in AITD, which may have significance for further understanding of the disease and the development of therapeutic approaches. Full article

Heap leaching is an economically favourable hydrometallurgical technique extensively employed in the mining industry for extracting valuable metals such as copper from low-grade ore deposits. This method renders a cost-effective solution for processing ore that would otherwise be considered uneconomical for conventional extraction techniques. This study investigates the efficiency of copper recovery from different particle size fractions of low-grade oxide ores that have undergone a crushing stage. Hydrochloric acid was used as a lixiviant in column heap leaching experiments to study the effect of particle size on copper extraction recovery. The experiments were conducted using column leach setups with dimensions of 150 mm in diameter and 2 m in height. Crushed ore samples, ranging in particle size from 25 mm down to 1.8 mm, were divided into 5 kg aliquots and loaded into the columns, with a total mass of approximately 40 kg per test. Leaching was performed over a period of 16 days using an acid concentration of 200 g/L. The results demonstrated promising copper recoveries. One sample achieved a copper extraction rate of 75% within 16 days, with maximum acid consumption reaching 23 kg/ton over 15 days. Another sample yielded a comparable copper recovery of 74% under the same timeframe but required a higher acid consumption rate of 30 kg/ton. Moreover, the consistent linear increase in copper recovery throughout the leaching period suggests minimal interference from pregnant solution robbing impurities in the ore that consumes the lixiviant. Full article

Background: Combat-related lower extremity injuries frequently require prolonged tourniquet application to control life-threatening hemorrhage. Although effective for hemorrhage control, prolonged ischemia followed by reperfusion substantially increases the risk of rhabdomyolysis, acute kidney injury (AKI), limb loss, and mortality. The optimal timing of anti-rhabdomyolysis infusion therapy in relation to tourniquet release remains uncertain. Methods: This retrospective single-center cohort study analyzed 120 Ukrainian military casualties with combat-related lower extremity injuries requiring prolonged tourniquet application and subsequent surgical management, including fasciotomy and tourniquet release. Patients were divided into two groups based on infusion strategy: standard therapy initiated after tourniquet release and early anti-rhabdomyolysis infusion therapy initiated before tourniquet removal during the ischemic phase. Primary outcomes included dialysis-requiring AKI, limb amputation, and death. Multivariable logistic regression models were adjusted for baseline physiological severity, including shock index at admission and baseline acid–base status. Model performance was evaluated using the Akaike Information Criterion (AIC) and receiver operating characteristic (ROC) analysis. Propensity score–based inverse probability of treatment weighting (IPTW) was applied as a sensitivity analysis. Results: After adjustment, early infusion therapy was independently associated with lower rates of dialysis-requiring AKI (adjusted odds ratio [OR] 0.33; 95% confidence interval [CI] 0.13–0.84; p = 0.020), limb amputation (OR 0.32; 95% CI 0.11–0.95; p = 0.040), and mortality (OR 0.23; 95% CI 0.07–0.77; p = 0.017). Adjusted models demonstrated good discriminative ability, with areas under the ROC curve of 0.813 for AKI, 0.838 for amputation, and 0.823 for mortality. Sensitivity analyses using IPTW yielded consistent results. Conclusions: In combat-related lower extremity injuries requiring prolonged tourniquet application, early initiation of anti-rhabdomyolysis infusion therapy prior to reperfusion is associated with significantly reduced risks of severe AKI, limb loss, and death. These findings suggest that preventive renal-protective strategies initiated before tourniquet release may improve outcomes in high-risk military trauma settings and warrant further prospective investigation. Full article

Background: Eating disorders (EDs) frequently co-occur with trauma-related symptoms and elevated autistic traits (ATs), both of which contribute to clinical complexity. Social camouflaging, strategies used to mask or compensate for ATs, has been increasingly described in ED populations, yet its relationship with trauma-related symptoms remains poorly understood. This study aimed to examine the association between social camouflaging and post-traumatic stress symptoms in individuals with EDs and to evaluate whether trauma-related symptomatology is associated with camouflaging behaviors. Methods: A total of 67 ED patients were assessed using the Adult Autism Subthreshold Spectrum, the Trauma and Loss Spectrum—Self Report (TALS-SR), the Camouflaging Autistic Traits questionnaire (CAT-Q), and the Eating Disorder Inventory (EDI-2). Participants were divided into high-trauma-symptoms (HTS) (N = 36, 53.7%) and low-trauma-symptoms (LTS) (N = 31; 46.3%) groups based on TALS-SR criteria. Results: The sample was predominantly female (92.5%), and gender distribution differed between groups, which may represent a potential confounding factor and limits the generalizability of the findings. The HTS group reported significantly higher TALS-SR, EDI-2, CAT-Q, and AdAS Spectrum scores, although for the latter the p-value was barely significant (p = 0.046). No differences emerged in the distribution of ED diagnoses between groups. CAT-Q scores were significantly positively correlated with TALS-SR total scores and with domains related to reaction to losses, maladaptive coping, avoidance/numbing, and personal vulnerability. Regression analyses showed that overall trauma-related symptoms were significantly associated with greater camouflaging; however, the proportion of explained variance was modest, suggesting that trauma-related symptoms represent only one of multiple factors linked to camouflaging. Conclusions: Among individuals with EDs, higher trauma-related symptomatology is linked to greater use of social camouflaging strategies. These findings suggest that camouflaging may represent a transdiagnostic correlate connecting neurodevelopmental vulnerability and trauma responses within ED populations, underscoring the importance of integrated assessment and trauma-informed care. Full article

The precision of data gathered from Ultra-Weak Fiber Bragg Grating (UWFBG) sensing technology is limited when measuring strain within asphalt pavements. To better understand its measurement mechanism and correct possible errors, this study examines the synergy deformation behavior between UWFBG and asphalt mixtures under loads. Initially, the mesoscopic model of asphalt mixture containing UWFBG was constructed using a discrete element model, followed by the validation of the model. Then, the propagation of microcracks at the interface between the asphalt mixture and UWFBG was analyzed, revealing damage characteristics of this material under various loading stages. Additionally, a quantitative relationship between the crack width and the monitoring strain was identified. The significant effect of introducing the sensor on crack propagation and interface debonding in strain response was also highlighted. The results indicate that when displacement exceeds 1.4 mm during a bending test, the number of both damage and microcracks increases markedly, with cracks progressively developing. Especially at the UWFBG interface subjected to a tensile load, microcrack growth rises sharply, leading to the failure of the interface. The mor-UWFBG interface is not the main damage location, but it is the most vulnerable location to damage and may be the one affecting the monitoring of UWFBG. Without sensors, a consistent linear relationship between monitoring strain and crack width is observed within the asphalt mixture. After introducing the UWFBG sensor, the strain-crack response of the asphalt mixture is divided into three stages: crack initiation, crack propagation, and interface debonding. When the crack width surpasses 0.03 mm, interface debonding significantly influences the strain growth rate, indicating the necessity of correcting the synergy deformation. Full article