Search Results (3,550)

(1) Background: IgA nephropathy (IgAN) is a leading cause of chronic kidney disease worldwide. Despite its prevalence, the molecular mechanisms of IgAN remain poorly understood, partly due to limited research scale. Identifying key genes involved in IgAN’s pathogenesis is critical for novel diagnostic and therapeutic strategies. (2) Methods: We identified differentially expressed genes (DEGs) by analyzing public datasets from the Gene Expression Omnibus. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate the biological roles of DEGs. Hub genes were screened using weighted gene co-expression network analysis combined with machine learning algorithms. Immune infiltration analysis was conducted to explore associations between hub genes and immune cell profiles. The hub genes were validated using receiver operating characteristic curves and area under the curve. (3) Results: We identified 165 DEGs associated with IgAN and revealed pathways such as IL-17 signaling and complement and coagulation cascades, and biological processes including response to xenobiotic stimuli. Four hub genes were screened: three downregulated (FOSB, SLC19A2, PER1) and one upregulated (SOX17). The AUC values for identifying IgAN in the training and testing set ranged from 0.956 to 0.995. Immune infiltration analysis indicated that hub gene expression correlated with immune cell abundance, suggesting their involvement in IgAN’s immune pathogenesis. (4) Conclusion: This study identifies FOSB, SLC19A2, PER1, and SOX17 as novel hub genes with high diagnostic accuracy for IgAN. These genes, linked to immune-related pathways such as IL-17 signaling and complement activation, offer promising targets for diagnostic development and therapeutic intervention, enhancing our understanding of IgAN’s molecular and immune mechanisms. Full article

Background/Objectives: The execution of clinical trials is increasingly constrained by operational complexity, regulatory requirements, and variability in site performance. These challenges have direct implications for the reliability of trial outcomes. However, standardized methods to evaluate site-level performance remain underdeveloped. This study introduces the Clinical Trial Site Performance Measure (CT-SPM), a novel framework designed to systematically capture site-level operational quality and to provide a scalable short form for routine monitoring. Methods: We conducted a multicenter study across six Italian academic hospitals (January–June 2025). Candidate performance indicators were identified through a systematic review and expert consultation, followed by validation and reduction using advanced statistical approaches, including factor modeling, ROC curve analysis, and nonparametric scaling methods. The CT-SPM was assessed for structural validity, discriminative capacity, and feasibility for use in real-world settings. Results: From 126 potential indicators, 18 were retained and organized into four domains: Participant Retention and Consent, Data Completeness and Timeliness, Adverse Event Reporting, and Protocol Compliance. A bifactor model revealed two higher-order dimensions (participant-facing and data-facing performance), highlighting the multidimensional nature of site operations. A short form comprising four items demonstrated good scalability and sufficient accuracy to identify underperforming sites. Conclusions: The CT-SPM represents an innovative, evidence-based instrument for monitoring trial execution at the site level. By linking methodological rigor with real-world applicability, it offers a practical solution for benchmarking, resource allocation, and regulatory compliance. This approach contributes to advancing clinical research by providing a standardized, data-driven method to evaluate and improve performance across networks. Full article

Background: Out-of-hospital cardiac arrest (OHCA) survivors demonstrate wide variation in neurological outcomes due to hypoxic–ischemic brain injury. Early prognostic stratification in the emergency department is essential to inform clinical decisions. This study aimed to improve the revised Cardiac Arrest Syndrome for Therapeutic hypothermia (rCAST) score by incorporating additional clinical variables and to evaluate its ability to predict poor neurological outcomes. Methods: This multicenter observational study analyzed OHCA survivors treated with targeted temperature management (TTM) between October 2015 and December 2018 at 22 university-affiliated hospitals participating in the Korean Hypothermia Network prospective registry. The primary outcome was poor neurological status at one month, defined as a Cerebral Performance Category (CPC) score of 3–5. Independent predictors were identified using multivariable logistic regression and incorporated into a modified rCAST (mCAST) score. Results: Among 881 included patients, age > 65 years (odds ratio [OR], 13.87; 95% confidence interval [CI], 7.38–26.08) and absence of brainstem reflexes (OR, 2.31; 95% CI, 1.29–4.12) were identified as independent predictors and added to the mCAST score. The mCAST demonstrated higher prognostic accuracy than the original rCAST (area under the curve [AUC], 0.849 vs. 0.823; p < 0.001). In the high-severity group, the mCAST identified a higher poor outcome rate (95.1% vs. 90.9%) while reducing the proportion of patients in this group (20.7% vs. 31.3%). Conclusions: The mCAST score improves early prognostic accuracy during the immediate post-cardiac arrest period by incorporating age and brainstem reflexes and may offer refined risk stratification without compromising clinical feasibility. Full article

Externally governed hydrogel swelling is a highly convenient yet inherently challenging process, as it requires both responsive materials and appropriately tuned external stimuli. In this work, for the first time, the influence of simultaneous action of external physical fields—ultrasound (US) and microwave heating (MW), combined with cooling—on the isothermal swelling kinetics of poly(methacrylic acid) (PMAA) hydrogel was investigated and compared with swelling under conventional thermal heating (TH) under isothermal conditions. Swelling kinetics were monitored over a temperature range of 248–318 K, under simultaneous cooling with either US, MW, or TH. The well-established Peppas model was used to determine swelling kinetics parameters, revealing a significant acceleration in the swelling process under MW (up to 48.8 times at 313 K), as well as different water penetrating mechanisms (non-Fickian diffusion) compared to TH and US (Super-case II). Additionally, it was demonstrated that the swelling conversion curves could be mathematically described using a “shrinking boundary surfaces” model. Isothermal swelling constants and the corresponding kinetic parameters (activation energy E_a and pre-exponential factor ln A) were calculated. The results confirmed that external physical fields significantly influence the thermal activation and swelling behavior of PMAA xerogels, offering insight into field-responsive transport processes in hydrogel networks. Full article

The Internet of Things (IoT) has surfaced as a revolutionary technology, enabling ubiquitous connectivity between devices and revolutionizing traditional lifestyles through smart automation. As IoT systems proliferate, securing device-to-device communication and server–client data exchange has become crucial. This paper presents a novel security framework that integrates elliptic curve cryptography (ECC) with artificial neural networks (ANNs) to enhance the Message Queuing Telemetry Transport (MQTT) protocol. Our study evaluated multiple machine learning algorithms, with ANN demonstrating superior performance in anomaly detection and classification. The hybrid approach not only encrypts communications but also employs the optimized ANN model to detect and classify anomalous traffic patterns. The proposed model demonstrates robust security features, successfully identifying and categorizing various attack types with 90.38% accuracy while maintaining message confidentiality through ECC encryption. Notably, this framework retains the lightweight characteristics essential for IoT devices, making it especially relevant for environments where resources are constrained. To our knowledge, this represents the first implementation of an integrated ECC-ANN approach for securing MQTT-based IoT communications, offering a promising solution for next-generation IoT security requirements. Full article

With the large-scale development of smart grids and the integration of renewable energy, the operational complexity and load volatility of power systems have increased significantly, placing higher demands on the accuracy and timeliness of electricity load forecasting. However, existing methods struggle to capture the nonlinear and volatile characteristics of load sequences, often exhibiting insufficient fitting and poor generalization in peak and abrupt change scenarios. To address these challenges, this paper proposes a deep learning model named CGA-LoadNet, which integrates a one-dimensional convolutional neural network (1D-CNN), gated recurrent units (GRUs), and a self-attention mechanism. The model is capable of simultaneously extracting local temporal features and long-term dependencies. To validate its effectiveness, we conducted experiments on a publicly available electricity load dataset. The experimental results demonstrate that CGA-LoadNet significantly outperforms baseline models, achieving the best performance on key metrics with an $R^2$ of 0.993, RMSE of 18.44, MAE of 13.94, and MAPE of 1.72, thereby confirming the effectiveness and practical potential of its architectural design. Overall, CGA-LoadNet more accurately fits actual load curves, particularly in complex regions, such as load peaks and abrupt changes, providing an efficient and robust solution for short-term load forecasting in smart grid scenarios. Full article

Internet of Things (IoT) networks are constantly exposed to various security challenges and vulnerabilities, including manipulative data injections and cyberattacks. Traditional security measures are often inadequate, overburdened, and unreliable in adapting to the heterogeneous yet diverse nature of IoT networks. This emphasizes the need for intelligent and effective methodologies. In recent times, deep learning models have been extensively used to monitor and detect intrusions in complex applications. The models can effectively learn and understand the dynamic characteristics of voluminous IoT datasets to prompt efficient decision-making predictions. This study proposes a hybrid Convolutional Neural Network and Gated Recurrent Unit (CNN-GRU) algorithm to enhance intrusion detection in IoT environments. The proposed CNN-GRU model is validated using two benchmark datasets: the IoTID20 and BoT-IoT intrusion detection datasets. The proposed model incorporates an effective technique to handle the class imbalance issues that are peculiar to voluminous datasets. The results demonstrate superior accuracy, precision, recall, F1-score, and area under the curve, with a reduced false positive rate compared to similar models in the literature. Specifically, the proposed CNN–GRU achieved up to 99.83% and 99.01% accuracy, surpassing baseline models by a margin of 2–3% across both datasets. These findings highlight the model’s potential for real-time cybersecurity applications in IoT networks and general industrial control systems. Full article

To address the building settlement issues induced by an urban geological hazard in a northern city, this study utilizes settlement monitoring data from 16 high-rise buildings. The non-uniform temporal data were processed using the Akima interpolation method to construct a settlement prediction model based on a backpropagation (BP) neural network. The model’s predictive performance was validated against traditional approaches, including the hyperbolic and exponential curve methods, and was further employed to estimate the stabilization time of building settlements. Additionally, spatiotemporal characteristics of settlement behavior under the influence of geological hazards were investigated through a comparative analysis of deformation data across the building group. The results demonstrate that the BP neural network model achieves a 58.3% improvement in predictive accuracy compared to traditional empirical methods, effectively capturing the settlement evolution of buildings. The model also provides reliable predictions for the time required for buildings to reach a stable state. The temporal evolution of building settlement exhibits a distinct three-stage pattern: (1) an initial abrupt phase dominated by rapid water and soil loss; (2) a rapid settlement phase primarily driven by the consolidation of sandy and clayey soils; and (3) a slow consolidation phase governed by the prolonged consolidation of cohesive soils. Spatially, building deformations show significant regional heterogeneity, and the existence of potential finger-like preferential pathways for water and soil loss appears to exert a substantial influence on differential settlements. Full article

This study presents a novel strategy for designing photocurable resins tailored for the additive manufacturing of smart thermoset materials. A quaternary formulation was developed by integrating bis(2-methacryloyl)oxyethyl disulfide (DADS) with an epoxy/thiol-ene system (ETES) composed of diglycidyl ether of bisphenol A (EP), pentaerythritol tetrakis(3-mercaptopropionate) (PTMP), and 4,4′-methylenebis(N,N-diallylaniline) (ACA4). This unique combination enables the simultaneous activation of four polymerization mechanisms: radical photopolymerization, thiol-ene coupling, thiol-Michael addition, and anionic ring-opening, within a single resin matrix. A key innovation lies in the exothermic nature of DADS photopolymerization, which initiates and sustains ETES curing at room temperature, enabling 3D printing without thermal assistance. This represents a significant advancement over conventional systems that require elevated temperatures or post-curing steps. The resulting hybrid poly(acrylate–co-ether–co-thioether) network exhibits enhanced mechanical integrity, shape memory behavior, and intrinsic self-healing capabilities. Dynamic Mechanical Analysis revealed a shape fixity and recovery of 93%, while self-healing tests demonstrated a 94% recovery of viscoelastic properties, as evidenced by near-overlapping storage modulus curves compared to a reference sample. This integrated approach broadens the design space for multifunctional photopolymers and establishes a versatile platform for advanced applications in soft robotics, biomedical devices, and sustainable manufacturing. Full article

We propose the Clustered Link-Prediction SEIRS model with Temporal Node Activation (CLP-SEIRS-T), a novel epidemiological framework that integrates community structure, link prediction, and temporal activation schedules to simulate malware propagation in urban communication networks. Unlike traditional static or homogeneous models, our approach captures the heterogeneous community structure of the network (modular connectivity), along with evolving connectivity (emergent links) and periodic device-usage patterns (online/offline cycles), providing a more realistic portrayal of how computer viruses spread. Simulation results demonstrate that strong community modularity and intermittent connectivity significantly slow and localize outbreaks. For instance, when devices operate on staggered duty cycles (asynchronous online schedules), malware transmission is fragmented into multiple smaller waves with lower peaks, often confining infections to isolated communities. In contrast, near-continuous and synchronized connectivity produces rapid, widespread contagion akin to classic epidemic models, overcoming community boundaries and infecting the majority of nodes in a single wave. Furthermore, by incorporating a common-neighbor link-prediction mechanism, CLP-SEIRS-T accounts for future connections that can bridge otherwise disconnected clusters. This inclusion significantly increases the reach and persistence of malware spread, suggesting that ignoring evolving network topology may underestimate outbreak risk. Our findings underscore the importance of considering temporal usage patterns and network evolution in malware epidemiology. The proposed model not only elucidates how timing and community structure can flatten or exacerbate infection curves, but also offers practical insights for enhancing the resilience of urban communication networks—such as staggering device online schedules, limiting inter-community links, and anticipating new connections—to better contain fast-spreading cyber threats. Full article

Background: Severe class imbalance limits reliable tabular AI in manufacturing, finance, and healthcare. Methods: We built a modular pipeline comprising correlation-aware seriation; a hybrid convolutional neural network (CNN)–transformer–Bidirectional Long Short-Term Memory (BiLSTM) encoder; variational autoencoder (VAE)-based minority augmentation; and deep/tree ensemble heads (XGBoost and Support Vector Machine, SVM). We benchmarked the Synthetic Minority Oversampling Technique (SMOTE) and ADASYN under identical protocols. Focal loss and ensemble weights were tuned per dataset. The primary metric was the Area Under the Precision–Recall Curve (AUPRC), with receiver operating characteristic area under the curve (ROC AUC) as complementary. Synthetic-data fidelity was quantified by train-on-synthetic/test-on-real (TSTR) utility, two-sample discriminability (ROC AUC of a real-vs-synthetic classifier), and Maximum Mean Discrepancy (MMD²). Results: Across five datasets (SECOM, CREDIT, THYROID, APS, and UCI), augmentation was data-dependent: VAE led on APS (+3.66 pp AUPRC vs. SMOTE) and was competitive on CREDIT (+0.10 pp vs. None); the SMOTE dominated SECOM; no augmentation performed best for THYROID and UCI. Positional embedding (PE) with seriation helped when strong local correlations were present. Ensembles typically favored XGBoost while benefiting from the hybrid encoder. Efficiency profiling and a slim variant supported latency-sensitive use. Conclusions: A data-aware recipe emerged: prefer VAE when fidelity is high, the SMOTE on smoother minority manifolds, and no augmentation when baselines suffice; apply PE/seriation selectively and tune per dataset for robust, reproducible deployment. Full article

Background/Objectives: The YSK olfactory function (YOF) test is a culturally adapted psychophysical tool that assesses threshold, discrimination, and identification. This study evaluated whether functional near-infrared spectroscopy (fNIRS) synchronized with routine YOF testing, combined with machine learning, can predict YOF subdomain performance in healthy adults, providing an objective neural correlate to complement behavioral testing. Methods: In this prospective diagnostic-accuracy (feasibility/validation) study in healthy adults with algorithm development, 100 healthy adults completed the YOF test while undergoing prefrontal/orbitofrontal fNIRS during odor blocks. Feature sets from ΔHbO/ΔHbR included time-domain descriptors, complexity (Lempel–Ziv), and information-theoretic measures (mutual information); the identification task used a hybrid attention–CNN. Separate models were developed for threshold (binary classification), discrimination (binary classification), and identification (binary classification). Performance was summarized with accuracy, area under the curve (AUC), F1-score, and (where applicable) sensitivity/specificity, using participant-level cross-validation. Results: The threshold classifier achieved accuracy 0.86, AUC 0.86, and F1 0.86, indicating strong discrimination of correct vs. incorrect threshold responses. The discrimination model yielded accuracy 0.75, AUC 0.76, and F1 0.75. The identification model (attention–convolutional neural network [CNN]) achieved accuracy 0.88, sensitivity 0.86, specificity 0.91, and F1 0.88. Feature-attribution (e.g., SHapley Additive exPlanations [SHAP]) provided interpretable links between fNIRS features and task performance for threshold and discrimination. Conclusions: Olfactory-evoked fNIRS signals can accurately predict YOF subdomain performance in healthy adults, supporting the feasibility of non-invasive, portable, near–real-time olfactory monitoring. These findings are preliminary and not generalizable to clinical populations; external validation in diverse cohorts is warranted. The approach clarifies the scientific essence of the method by (i) aligning psychophysical outcomes with objective hemodynamic signatures and (ii) introducing a feature-rich modeling pipeline (ΔHbO/ΔHbR + Lempel–Ziv complexity/mutual information; attention–CNN) that advances prior work. Full article

Nitrogen fertilization is decisive for maize productivity, fertilizer use efficiency, and sustainability, which calls for fast and nondestructive nutritional diagnosis. This study evaluated the classification of maize plant nutritional status from red, green, and blue (RGB) leaf images using texture attributes. A greenhouse experiment was conducted under a completely randomized factorial design with four nitrogen doses, one maize hybrid Pioneer 30F35, and four replicates, at two sampling times corresponding to distinct phenological stages, totaling thirty-two experimental units. Images were processed with the gray-level cooccurrence matrix computed at three distances 1, 3, and 5 pixels and four orientations 0°, 45°, 90°, and 135°, yielding eight texture descriptors that served as inputs to five supervised classifiers: an artificial neural network, a support vector machine, k nearest neighbors, a decision tree, and Naive Bayes. The results indicated that texture descriptors discriminated nitrogen doses with good performance and moderate computational cost, and that homogeneity, dissimilarity, and contrast were the most informative attributes. The artificial neural network showed the most stable performance at both stages, followed by the support vector machine and k nearest neighbors, whereas the decision tree and Naive Bayes were less suitable. Confusion matrices and receiver operating characteristic curves indicated greater separability for omission and excess classes, with D1 standing out, and the patterns were consistent with the chemical analysis. Future work should include field validation, multiple seasons and genotypes, integration with spectral indices and multisensor data, application of model explainability techniques, and assessment of latency and scalability in operational scenarios. Full article

Abnormal shutdown detection in oilfield pumping units presents significant challenges, including degraded image quality under low-light conditions, difficulty in detecting small or obscured targets, and limited capabilities for dynamic state perception. Previous approaches, such as traditional visual inspection and conventional image processing, often struggle with these limitations. To address these challenges, this study proposes an intelligent method integrating multi-scale feature enhancement and low-light image optimization. Specifically, a lightweight low-light enhancement framework is developed based on the Zero-DCE algorithm, improving the deep curve estimation network (DCE-Net) and non-reference loss functions through training on oilfield multi-exposure datasets. This significantly enhances brightness and detail retention in complex lighting conditions. The DAFE-Net detection model incorporates a four-level feature pyramid (P3–P6), channel-spatial attention mechanisms (CBAM), and Focal-EIoU loss to improve localization of small/occluded targets. Inter-frame difference algorithms further analyze motion states for robust “pump-off” determination. Experimental results on 5000 annotated images show the DAFE-Net achieves 93.9% mAP@50%, 96.5% recall, and 35 ms inference time, outperforming YOLOv11 and Faster R-CNN. Field tests confirm 93.9% accuracy under extreme conditions (e.g., strong illumination fluctuations and dust occlusion), demonstrating the method’s effectiveness in enabling intelligent monitoring across seven operational areas in the Changqing Oilfield while offering a scalable solution for real-time dynamic anomaly detection in industrial equipment monitoring. Full article

Assessing seismic vulnerability and prioritizing railway tunnels for seismic rehabilitation are critical components of railway infrastructure management, especially in seismically active regions. This study focuses on a railway network in Northwest Iran, consisting of 103 old masonry rock tunnels. The vulnerability of these tunnels is evaluated under 12 active faults as seismic sources. Fragility curves derived from the HAZUS methodology estimate the probability of various damage states under seismic intensities, including peak ground acceleration (PGA) and peak ground displacement (PGD). The expected values of the damage states are computed as the damage index (DI) to measure the severity of damage. A normalized prioritization index (NPI) is proposed, considering seismic vulnerability and life cycle damages in tunnel prioritizing. Finally, a detailed prioritization is provided in four classes. The results indicate that 10% of the tunnels are classified as priority, 33% as second priority, 40% as third priority, and 17% as fourth priority. This prioritization is necessary when there are budget limitations and it is not possible to retrofit all tunnels simultaneously. The main contribution of this study is the development of an integrated, data-driven framework for prioritizing the seismic rehabilitation of aging masonry railway tunnels, combining fragility-based vulnerability assessment with life-cycle damage considerations in a high-risk and data-limited region. The framework outlined in this study enables decision-making organizations to efficiently prioritize the tunnels based on vulnerability, which helps to increase seismic resilience. Full article