Search Results (165)

Our previous study has shown that supplementation of 0.50% benzoic acid (BA) increased growth performance, promoted rumen fermentation, and improved the composition and function of rumen microbiota. This research was designed to conduct a deeper exploration of the impacts of dietary supplementation with BA on the apparent digestibility of nutrients and the composition of rectal microbiota in weaned Holstein dairy calves. Sixteen Holstein heifer calves with similar body weights (91.2 ± 0.7 kg) were selected and randomly allocated into two groups, each comprising eight calves. Calves in the control group (CON group) were fed with a basal diet, while those in the benzoic acid group (BA group) were fed with the basal diet supplemented with 0.50% benzoic acid (on a dry matter basis). The experimental period started at 60 days of age and ended at 102 days of age, lasting for a total of 42 days. The calves were weaned at 60 days of age, with a transition period of 7 days. Feed samples were collected every two weeks, fecal samples were collected from 99 to 101 days of age, and blood samples were collected at 102 days of age. The results showed that supplementation with BA did not influence the digestibility of dry matter, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, calcium, and phosphorus between the two groups. Compared with the CON group, BA supplementation tended to decrease the total cholesterol (TC) in the serum of the calves (p = 0.067). Supplementation with BA increased the relative abundances of the two beneficial bacteria, Bifidobacterium and Bifidobacterium pseudolongum (p < 0.05, LDA > 2), but decreased that of the harmful bacterium, Clostridium sensu stricto 1, in the rectum of dairy calves. The microbial functional prediction revealed that the fecal microbial metabolism involved in primary bile acid biosynthesis was higher in the calves from the BA group. In conclusion, the present study demonstrated that adding 0.50% BA to the diet did not influence the apparent nutrient digestibility, but improved rectal microbiota health, which finally promoted the growth performance in weaned Holstein dairy calves. Full article

The Red Sea is rich in symbiotic microorganisms that have been identified as sources of bioactive compounds with antimicrobial, antifungal, and antioxidant properties. In this study, we aimed to explore the potential of marine sponge-associated bacteria as sources of antibacterial compounds, emphasizing their significance in combating antibiotic resistance (AMR). The crude extracts of Micrococcus, Bacillus, and Staphylococcus saprophyticus exhibited significant antibacterial activity, with inhibition zones measuring 12 mm and 14 mm against Escherichia coli, Staphylococcus aureus, Candida albicans, and other infectious strains. The DPPH assay showed that the bacterial isolates AN3 and AN6 exhibited notable antioxidant activity at a concentration of 100 mg/mL. To characterize the chemical constituents responsible for the observed bioactivity, a GC–MS analysis was performed on ethyl acetate extracts of the potent strains. The analysis identified a range of antimicrobial compounds, including straight-chain alkanes (e.g., Tetradecane), cyclic structures (e.g., Cyclopropane derivatives), and phenolic compounds, all of which are known to disrupt microbial membranes or interfere with metabolic pathways. The bioprospecting and large-scale production of these compounds are challenging. In conclusion, this study underscores the potential for marine bacteria associated with sponges from the Red Sea to be a source of bioactive compounds with therapeutic relevance. Full article

Pear black spot disease seriously threatens the pear industry. Currently, its control mainly relies on chemical fungicides while biological control using antagonistic microorganisms represents a promising alternative approach. This study identified and characterized Bacillus velezensis TRMB57782 as a biocontrol strain through whole-genome sequencing. AntiSMASH analysis predicted the strain’s potential to produce secondary metabolites such as surfactin, difficidin, and bacilysin. In vitro experiments demonstrated that TRMB57782 inhibited the growth of Alternaria gaisen. In vivo experiments using excised branches and pear fruits at two different stages also showed significant control effects. A preliminary exploration of the metabolic substances of TRMB57782 was carried out. The strain can produce siderophores and three biocontrol enzymes. Crude extracts obtained by the hydrochloric acid precipitation and ammonium sulfate saturation precipitation of the bacterial liquid exhibited significant activity and volatile organic compounds showed biocontrol activity. Meanwhile, the effects of strain TRMB57782 on the hyphae of pathogenic fungi were studied, leading to hyphal atrophy and spore shrinkage. This paper provides an effective biocontrol strategy for fragrant pear black spot disease, reveals the antibacterial mechanism of Bacillus velezensis TRMB57782, and offers a new option for the green control of pear black spot disease. Full article

The sharp rise in the worldwide production of biodiesel has created an excess in the crude glycerol market, so it is essential to develop new added-value alternatives for crude glycerol. This paper describes a study on fermenting high concentrations of two types of medium-pure crude glycerol to solvents by Clostridium pasteurianum. The effect of media composition (iron, yeast extract, and vitamins) on solvents production was assessed by a full factorial design with pure glycerol. Granular activated carbon (GAC) adsorption was highly effective in removing impurities from crude glycerol. Following GAC pretreatment, fermentation of glycerol at initial concentration as high as 60 g L⁻¹ was possible, resulting in a butanol production of ~9 g L⁻¹. Based on these results, a batch fermentation with in situ gas stripping and pH controlled at ≥6.5 was shown to be the best alternative to enhance biomass growth, glycerol uptake, and solvent production. The combination of controlling pH in the early stages of fermentation with in situ butanol removal stabilised the metabolism of the strain and showed that the fermentation performance with crude glycerol is very similar to that of pure glycerol. With a notable uptake of glycerol (>83%), solvent production was >11 g L⁻¹ butanol (yield > 0.21 g g⁻¹_{glycerol consumed}) and >6 g L⁻¹ 1,3-propanediol (yield > 0.13 g g⁻¹_{glycerol consumed}). Setting the fermentation conditions to achieve a high uptake of high levels of glycerol with a similar product distribution is of great interest for the viability of the industrial processing of crude glycerol into chemicals via biological conversion. Full article

Diabetes mellitus (DM) is a multifactorial metabolic disorder characterized by chronic hyperglycemia and systemic metabolic dysregulation. Although ginsenosides, the primary bioactive components of Panax ginseng Meyer, exhibit regulatory effects on glucose and lipid metabolism, their precise mechanisms and key targets in DM remain incompletely understood. Unlike previous studies focusing solely on crude extracts or individual ginsenosides, this study integrates network pharmacology, molecular docking, and molecular dynamics (MD) simulations to systematically elucidate the multi-target mechanisms of ginsenosides, with experimental validation using the ginsenoside derivative AD-1. Network pharmacology identified 134 potential targets, with protein–protein interaction (PPI) analysis revealing 25 core targets (such as NFKB1, HDAC1, ESR1, and EP300). Molecular docking and MD simulations showed that ginsenosides have stable binding conformations with these targets and exhibit excellent dynamic stability. Notably, in vivo experiments using AD-1 in streptozotocin-induced type 1 diabetic mice confirmed its therapeutic efficacy, significantly downregulating key diabetic markers (e.g., NFKB1 and HDAC1) in pancreatic tissues—a finding unreported in prior studies. This study not only revealed the multitarget pharmacological mechanism of ginsenosides but also highlighted the therapeutic potential of AD-1. These findings provide a foundation for further mechanistic studies and suggest new strategies for the application of novel ginsenoside derivatives in diabetes therapy. Full article

Improving the utilization of spent mushroom substrate and enhancing the digestibility of straw-based feed are critical for promoting environmental sustainability. However, the effects of replacing sawdust with straw in the cultivation of Pleurotus ostreatus—including changes in physicochemical properties, enzyme activities, and microbial community structure and function—remain unclear. In this study, corn straw was used as the substrate for P. ostreatus cultivation. Dynamic changes during the fermentation process were investigated through analyses of biological growth characteristics, physicochemical properties, enzyme activities, and amplicon sequencing. The results indicated a significant increase in mushroom yield, with the M80% treatment group achieving a yield of 156.09 ± 7.15 g. The nutritional value of the fermented feed was markedly improved; after 50 days of fermentation, crude protein (CP) and ether extract (EE) contents increased by 5.42% and 0.79%, respectively, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents decreased by 18.5% and 22.3%, compared to day 0. Activities of cellulase, xylanase, and laccase were also elevated, contributing to more effective lignocellulose degradation. Furthermore, Illumina sequencing revealed shifts in bacterial and fungal metabolic pathways. The fungal community was dominated by Ascomycota and Basidiomycota, with Pleurotus as the prevailing genus, while the bacterial community was mainly composed of antagonistic genera such as Bacillus and Bacteroides. These findings provide a theoretical basis for understanding the role of microbial interactions during straw substrate fermentation in improving feed quality and increasing P. ostreatus yield. Full article

High-value-added biomolecules such as phenolic compounds and flavonoids from secondary metabolism and macromolecules such as sugars are the main constituents of several plants. Thus, this work aims to optimize the extraction of these biomolecules present in the pods of Cassia grandis L.f. Initially, the effect of choline-based ionic liquids—ILs (choline chloride [Ch]Cl, dihydrogen citrate [Ch][DHC], and bitartrate [Ch][BIT]) as extracting agents for phenolic compounds and flavonoids was evaluated based on their efficiency and selectivity. Then, a 2³ full factorial design with six central points was performed using the IL concentration, the solid–liquid ratio, and the temperature as independent variables. The extract obtained in the best condition was subjected to pervaporation, after which the concentrates and the crude extract were used to determine the physical properties (density, viscosity, and refractive index). The hydrophobic–hydrophilic balance of the extracting agent and the biomolecules are the extraction process’s driving force. The best extraction condition was formed by [Ch][DHC] at 15 wt%, with a solid–liquid ratio of 1:15, at 45 °C for 30 min, resulting in 153.71 ± 5.81 mg·g⁻¹ of reducing sugars; 483.51 ± 13.10 mg·g⁻¹ of total sugars; 11.79 ± 0.54 mg·g⁻¹ of flavonoids; and 38.10 ± 2.90 mg·g⁻¹ of total phenolic compounds. All the physical properties of the biomolecules are temperature-dependent; for density and refractive index, a linear correlation is observed, while for viscosity, the correlation is exponential. Increasing the temperature decreases all properties, and the extract concentration for 8× presents the highest values of density (1.283 g·cm⁻³), viscosity (9224 mPa·s), and refractive index (1.467). Full article

Citrus lumia Risso is an ancient, cultivated Mediterranean lime belonging to the Rutaceae family. It is a species extremely difficult to retrieve, but it is still found in some private gardens in certain regions of Southern Italy. Citrus fruits are a rich source of bioactive compounds, particularly polyphenols, which have been linked to a reduction in the risk of several metabolic diseases. Here, hesperidium peel extracts were obtained by maceration with ethanol:water mixtures in different proportions (50:50, 80:20, 0:100) and the resulting crude extracts were then passed through a glass column containing adsorbent resins to concentrate the polyphenolic compounds. After phytochemical characterization, the extracts were evaluated for antioxidant activity using electron paramagnetic resonance (EPR) spectroscopy. Finally, the water polyphenolic-rich extract (ClumWp), which was the extract with the highest flavonoid content (18.355 ± 1.607 mg/mL) and the strongest antioxidant activity against hydroxyl radical, was tested to evaluate its potential protective effects on lipid accumulation in both 2D hepatocyte cultures and 3D spheroids. Treatment with 25 and 50 μg/mL resulted in a reduction in intracellular lipid content in the HepG2 liver cell line, while treatment with 100 µg/mL ClumWp resulted in a reduction in the intracellular lipid content in HepG2 + LX2 spheroids. In addition, treatment with ClumWp significantly increased ATP levels in the spheroids compared to those untreated, suggesting its ability to restore and promote ATP production. Our results highlight that the study of neglected species, such as Citrus lumia Risso, remains a valuable opportunity to valorize Mediterranean biodiversity, especially in the context of its potential applications to improve human health. In particular, the polyphenolic fraction of Citrus lumia peel showed promising effects on lipid metabolism and cellular energy balance and may prove valuable in the treatment of metabolic disorders such as MASLD, where lipid accumulation disrupts normal cellular functions. Full article

Background/Objectives: Argania spinosa L. Skeels is a Moroccan endemic plant widely used by the local population as folk medicine. This study aimed to investigate the effects of Argan fruit pulp on lipid metabolism disorders and liver steatosis in hypercaloric diet-fed mice. Methods: Animals were treated with the Argan fruit pulp extract and its fractions for 12 weeks at 100 and 200 mg Kg⁻¹ BW daily. The analysis was conducted on lipid levels in plasma, liver, feces, and bile as well as on glycemia. The liver glutathione, malondialdehyde, and antioxidant enzyme activities were assessed. The hepatic steatosis was evaluated by measuring transaminases and alkaline phosphatase activities and examining histological sections. The polyphenol profiles were determined using HPLC-DAD. Possible underlying mechanisms in the hypolipidemic and hepatoprotective activities were predicted by molecular docking. Results: The crude extract and its aqueous fraction (rich in protocatechuic and gallic acids) significantly restored plasma lipids and glucose levels. Indeed, total cholesterol level (TCHO) was decreased in the liver but increased in bile and feces. The treatment also reduced body weight and liver and adipose tissue mass and prevented liver steatosis. The ethyl acetate fraction exhibited no effect on lipid metabolism but significantly prevented liver oxidative stress. The crude extract and its fractions appear to be nontoxic (LD50 > 5000 mg Kg⁻¹) in mice. The phenolic acids demonstrated strong binding affinity to key targets involved in regulating lipid homeostasis, including ABCA-1, LXR, CYP7A1, HMH-CoA reductase, and PCSK-9. However, the identified flavonoids exhibited high affinities to targets involved in oxidative stress defense (SOD, CAT, and CYP2E1). Conclusions: The Argan fruit pulp, particularly its polyphenols, could be a promising natural approach for preventing cardio-metabolic diseases by improving lipid metabolism and reducing liver oxidative stress. Full article

Due to the strong polarity and volatility of organic acids, there is often a lack of effective quantitative methods for organic acids in vivo or in vitro. This study introduced a robust and scientifically validated LC-MS methodology for quantitatively analyzing six organic acids, encompassing five short-chain fatty acids and lactate, observed in in vitro fermentation and human fecal samples. This method was able to achieve precise quantification through the monitoring of mass-to-charge (m/z) ratios of the deprotonated negative ions. After optimization, a 2:1 (v/v) dichloromethane/acetonitrile mixture was utilized to extract the crude acid mix from fermentation or fecal samples. Subsequently, the organic acids were isolated using 3 mL of 3.5 M ammonium hydroxide solution from mixtures. The calibration curves for six organic acids demonstrated linearity with R² > 0.991 across the concentration ranges of 0.01–5.0 or 0.01–20 mM. The coefficient of variation and accuracy were 2–13% and 95–128%, respectively, which exhibited good precision and sensitivity. Accurate quantification of organic acids would aid in metabolic analysis in bacterial culture supernatants and human fecal matter. Full article

The endocarp of Exocarpium Citri Grandis (ECG) is abundant in various bioactive components, such as polysaccharides; however, there are few studies on them. Thus, it is highly necessary to carry out further research on the structural characterization and biological activities of ECG polysaccharides (EPs), which are important bioactive substances. In this study, water-extracted EPs were precipitated by ethanol with final concentrations of 50%, 70%, and 90% (v/v), respectively. Three crude polysaccharides (EP50, EP70, and EP90) were fractioned successively. The three polysaccharide fractions were structurally elucidated and were investigated in vitro for their biological activities related to glucose metabolism containing inhibitory effects on α-glucosidase and non-enzymatic glycosylation and their antioxidant capacities. The main results are summarized as follows: (1) Gradient ethanol precipitation and physicochemical properties of EPs: The yields of EP50, EP70, and EP90 were 11.18%, 0.57%, and 0.18%, respectively. The total sugar contents were 40.01%, 52.61%, and 53.46%, and the uronic acid contents were 30.25%, 18.11%, and 8.17%, respectively. In addition, the three fractions had the same composition of monosaccharides, including rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid, with differences in the content of neutral and acidic monosaccharides. They all may be branched polymers and spherical conformation, and they were acidic polysaccharides containing esterified and non-esterified uronic acids, pyranose-form sugars, and glycosidic linkages of α-configuration and β-configuration, with esterification degrees of 32.25%, 28.82%, and 15.58%, respectively. Meanwhile, EP50, EP70, and EP90 were mainly amorphous, and the molecular conformation in solution was a spherical branching polymer without a triple helix structure. The EPs exhibited excellent thermal stability, with their structures remaining stable below 170 °C. (2) In terms of activity research, the results showed that EPs had a good α-glucosidase inhibitory effect with IC₅₀ values of 1.17 mg/mL, 1.40 mg/mL, and 2.72 mg/mL, respectively, among which EP50 was the best. EP50, EP70, and EP90 displayed antioxidant activity by scavenging DPPH and ABTS radicals as well as oxygen radical absorbance capacity. Among them, EP90 had the strongest antioxidant activity. Furthermore, the EPs showed prominent effects on the inhibitory activity of non-enzymatic glycosylation. In summary, the research on the extraction of polysaccharide from ECG provides a technical reference for the further utilization of ECG resources. This study on antioxidant activity provides theoretical support for their use as a natural antioxidant. As oxidation and glycation are relevant to diabetic complications, the result of this work suggests that EPs may be effective in preventing and treating diabetic complications. Full article

Mikania micrantha, one of the world’s most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment—particularly the role of flavonoids in shaping soil microbial communities—remain underexplored. In this study, we identified and quantified ten flavonoids from M. micrantha root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin. To examine their impact, crude flavonoid extracts were applied to soil in potted experiments, which demonstrated that these compounds significantly increased soil fungal diversity and boosted the relative abundance of arbuscular mycorrhizal fungi (AMF). Furthermore, KEGG pathway analysis revealed that flavonoid addition elevated the copy numbers of genes involved in nitrogen cycling and metabolic functions, enhancing nutrient availability and microbial activity. Additionally, crude flavonoid extracts promoted the relative abundance of beneficial soil bacteria, such as Achromobacter, as well as AMF, both of which contribute to nutrient acquisition, plant growth, and soil health. These findings indicate that M. micrantha’s flavonoids can alter soil microbial community composition, thereby creating a favorable environment that reinforces its competitive edge over native plants. Full article

Natural compounds derived from microorganisms, especially those with antioxidant and anticancer properties, are gaining attention for their potential applications in biomedical, cosmetic, and food industries. Marine fungi, such as Asteromyces cruciatus, are particularly promising due to their ability to produce bioactive metabolites through the degradation of marine algal polysaccharides. This study investigates the metabolic diversity of A. cruciatus grown on different carbon sources: glucose, Durvillaea spp., and Macrocystis pyrifera. Crude extracts of fungal biomass were analyzed for total phenolic content (TPC), antioxidant capacity (TAC), toxicity, and phenolic compound identification using ultra-high-performance liquid chromatography coupled with high-resolution electrospray ionization mass spectrometry (UHPLC-MS/MS). The analysis revealed the presence of anthraquinone compounds, including emodin (0.36 ± 0.08 mg/g DW biomass) and citrereosein in glucose medium and citrereosein and endocrocin in M. pyrifera medium. No such compounds were detected in Durvillaea spp. medium. The glucose-grown extract exhibited the highest TPC (3.09 ± 0.04 mg GAE/g DW) and TAC (39.70 ± 1.0 µmol TEq/g biomass). Additionally, no detrimental effects were observed on a neuronal cell line. These findings highlight the influence of carbon sources on the production of bioactive metabolites and their functional properties, providing valuable insights into the biotechnological potential of A. cruciatus. Full article

Phycocyanin is a water-soluble pigment protein extracted from prokaryotes such as cyanobacteria and has strong antioxidant activity. As a silage additive, it is expected to enhance the antioxidant activity and fermentation quality of alfalfa silage. This study revealed the effects of different proportions of phycocyanin (1%, 3%, 5%) on the quality, bacterial community and antioxidant capacity of alfalfa silage. The results showed that 5% phycocyanin supplementation could maintain dry matter (DM), crude protein (CP) and water-soluble carbohydrate (WSC) content; increase lactic acid (LA) content; decrease pH and butyric acid (BA) and ammonia nitrogen (NH₃-N) content; and improve fermentation quality. At the same time, the contents of total antioxidant capacity (TAOC), total phenol content (TP), polysaccharide content (P) and total flavonoid content (F) in the addition group were significantly increased, the antioxidant capacity was enhanced and the abundance of lactic acid bacteria was increased, which was positively correlated with silage quality. Phycocyanin can improve the metabolic function of carbohydrates and amino acids and promote the production of secondary metabolites. The application of phycocyanin broadens the variety of additives for alfalfa silage. Full article

Fungal infections pose a growing public health threat, creating an urgent clinical need for new antifungals. Natural products (NPs) from organisms in extreme environments are a promising source for novel drugs. Streptomyces albidoflavus CBMAI 1855 exhibited significant potential in this regard. This study aimed to (1) assess the antifungal spectrum of the CBMAI 1855 extract against key human pathogens, (2) elicit NP production through co-cultivation with fungi, correlating the metabolites with the biosynthetic gene clusters (BGCs), and (3) perform in silico toxicity predictions of the identified compounds to analyze their suitability for drug development. The crude extract of CBMAI 1855 exhibited broad-spectrum antifungal activity. The metabolomic analysis identified antifungal NPs such as antimycin A, fungimycin, surugamides, 9-(4-aminophenyl)-3,7-dihydroxy-2,4,6-trimethyl-9-oxo-nonoic acid, and ikarugamycin, with the latter two predicted to be the most suitable for drug development. Genome mining revealed three cryptic BGCs potentially encoding novel antifungals. These BGCs warrant a detailed investigation to elucidate their metabolic products and harness their potential. CBMAI 1855 is a prolific producer of multiple antifungal agents, offering a valuable source for drug discovery. This study highlights the importance of exploring microbial interactions to uncover therapeutics against fungal infections, with a detailed exploration of cryptic BGCs offering a pathway to novel antifungal compounds. Full article