Search Results (3,524)

Background: The (COVID-19) pandemic profoundly disrupted daily routines and physical activity (PA), especially among university students, due to restrictions and limited access to sports facilities. As this group is particularly vulnerable to sedentary lifestyles and mental health issues, understanding their PA patterns is crucial. This study explores overall and domain-specific PA levels and the influence of sociodemographic factors, offering insights for promoting sustainable PA strategies in higher education during and beyond health crises. Methods: A cross-sectional online survey was conducted among University of Foggia students during the pandemic. The participants completed the validated Italian IPAQ-Long to assess PA across various domains. Associations with demographics and perceived barriers were analyzed via t tests, ANOVA, and nonparametric tests. Results: A total of 301 students completed the survey. Despite barriers such as limited living space, low income, and sports facility closures, 66% of the participants reported high PA levels, mainly through work-related and leisure activities. This remains insufficient. PA varied significantly by gender, income, residence, and employment status: males reported higher leisure PA, whereas females engaged more in active transport and domestic activities. Rural residents and those with moderate incomes demonstrated higher overall PA, whereas employed students presented lower activity levels. These findings underscore the complex socioeconomic and environmental factors shaping PA behavior during an unprecedented global health crisis. Conclusions: The findings reveal that students’ resilience in maintaining PA is a coping mechanism despite socioeconomic and environmental barriers. Tailored, accessible PA initiatives integrated into university curricula can enhance student well-being, academic performance, and long-term health during and after public health emergencies. Universities should adopt accessible, equity-oriented PA initiatives to promote physical and mental health and enhance public-health preparedness during future emergencies. Full article

Whole-slide histology images (WSIs) can exceed 100 k × 100 k pixels, making direct pixel-level segmentation infeasible and requiring patch-level classification as a practical alternative for downstream WSI segmentation. However, most approaches either treat patches independently, ignoring spatial and biological context, or rely on deep graph models prone to oversmoothing and loss of local tissue detail. We present WSI-GT (Pseudo-Label Guided Graph Transformer), a simple yet effective architecture that addresses these challenges and enables accurate WSI-level tissue segmentation. WSI-GT combines a lightweight local graph convolution block for neighborhood feature aggregation with a pseudo-label guided attention mechanism that preserves intra-class variability and mitigates oversmoothing. To cope with sparse annotations, we introduce an area-weighted sampling strategy that balances class representation while maintaining tissue topology. WSI-GT achieves a Macro F1 of 0.95 on PATH-DT-MSU WSS2v2, improving by up to 3 percentage points over patch-based CNNs and by about 2 points over strong graph baselines. It further generalizes well to the Placenta benchmark and standard graph node classification datasets, highlighting both clinical relevance and broader applicability. These results position WSI-GT as a practical and scalable solution for graph-based learning on extremely large images and for generating clinically meaningful WSI segmentations. Full article

Background/Objectives: Sex and gender influence dietary habits, eating behaviors, mental health, and obesity risk. Women exhibit a higher prevalence of emotional eating and mental health problems, which may contribute to sex-specific differences in adiposity. This study aimed to explore the associations between adiposity, diet quality, eating behaviors, mental health, and well-being, and to examine whether eating behaviors mediate the relationship between mental health and adiposity, stratified by sex. Methods: One hundred twenty-three adults (35.6 ± 7.9 years; 63.4% women) with overweight and obesity participated in this cross-sectional study. Adiposity parameters (BMI, body fat, waist and hip circumferences), biochemical parameters, eating behaviors (Three-Factor Eating Questionnaire-R21), well-being (WHO-5), perceived stress (Perceived Stress Scale), diet quality (17-item MedDiet questionnaire), and physical activity (International Physical Activity Questionnaire) were evaluated. Linear regression and path analyses were used to examine associations and mediation effects. Results: Women reported higher emotional eating and cognitive restraint scores (p = 0.017 and p = 0.034, respectively) and greater adherence to the Mediterranean diet (p < 0.001) than men. In men, well-being was positively associated with diet quality, while higher stress, cognitive restraint, and poorer diet quality were linked to greater adiposity. In women, well-being and diet quality were inversely associated with adiposity, while emotional and uncontrolled eating were related to higher adiposity and poorer biochemical markers. Emotional and uncontrolled eating mediated the relationship between well-being and adiposity only in women. Conclusions: Our results underscore the importance of incorporating sex- and gender-sensitive approaches in obesity prevention and treatment. For women, interventions should focus on emotional regulation and coping strategies, whereas for men, improving diet quality may be more effective. Full article

Sex differences in stress response continue to be understudied in basic physiological and behavioral research. The current study aimed to investigate the sex-specific effects of chronic stress in wild-derived house mice subjected to chronic unpredictable stress (CUS). The use of wild-derived mice enhanced the ecological validity of our stress model. We applied CUS for 5 weeks based on protocols previously established in laboratory mice, with regular weighting and welfare checks. Control mice were not subjected to stress. After the 5-week exposure, behavioral tests were performed, blood and hair samples were collected for corticosterone measurement, and stress-sensitive organ weights were assessed. Stressed females, but not stressed males, gained significantly less body weight over the entire CUS period. After CUS, mice tended to have higher adrenal and thymus weights. In stressed females, we observed significantly prolonged grooming time in the open field test and fewer immobility episodes in the tail suspension test (TST). Stressed males displayed significantly shorter immobility time in TST. Stressed males, but not stressed females, had significantly higher levels of hair corticosterone, with a similar tendency in plasma. Our results indicate different CUS coping strategies in males and females and raise a question about the development of different protocols for the assessment of stress responses in males and females. Full article

The rapid transformation of the 21st-century labour market requires students to be highly psychologically adaptable, especially in dual education systems where academic and work-based learning occur simultaneously. This study examines resilience as a psychological and pedagogical protective factor among students in dual vocational education and dual higher education programmes. Using a quantitative research design with validated scales measuring resilience, motivation, satisfaction, and stress, the research investigates how individual and contextual factors influence students’ adaptability. The results showed that vocational education and training students exhibited greater resilience, greater learning satisfaction, and lower levels of stress than those in higher education. Regression analysis confirmed that resilience positively contributes to academic success, while supportive mentoring and a structured learning environment enhance emotional stability and motivation. The analysis highlights that autonomy and pressure to perform are associated with higher levels of stress in higher education, underscoring the critical role of mentorship and peer support in improving adaptability. These findings emphasise that resilience is not only an individual capacity, but also a pedagogical and organisational construct; its systematic development should be integrated into the dual education framework to support student well-being, learning effectiveness, and long-term professional adaptation. Full article

Background/Objectives: Emotional eating is defined as a coping mechanism characterized by food consumption in response to negative emotions, and it typically involves overconsumption and a preference for energy-dense and highly palatable foods. The aim of this study was to analyze the influence of experienced stress and emotional eating on body mass in a population of Polish female adolescents. Methods: This study included 816 participants (aged 15–20 years) recruited within the nationwide PLACE-19 Study using random quota sampling of Polish secondary schools, and data were collected by the Computer-Assisted Web Interview (CAWI). Perceived stress was measured with the Adolescent Stress Questionnaire (ASQ), emotional eating was measured with the Emotional Eating Subscale (EE-3) of the Three-Factor Eating Questionnaire (TFEQ), and self-reported body mass was also recorded. Body mass was interpreted either using standard BMI values for adults or growth reference values for minors. Results: Adolescents with excessive body weight reported higher levels of stress on the peer pressure (p = 0.0011 for continuous variables; p = 0.0016 for categories) and financial pressure component scales (p = 0.0319 for continuous variables) than their normal-weight and underweight counterparts. They also displayed higher emotional eating scores across all subscales and for the total emotional eating score (p < 0.05 for continuous variables), particularly for anxiety (p = 0.0345 for categories). The association was confirmed within mediation analysis, as the direct influence explained 79% of the influence of stress on body mass, and the indirect influence, mediated by emotional eating, explained 21% of the influence of stress on body mass. Conclusions: Adolescents with excessive body weight are more prone to stress and emotional eating. The stress itself affects body weight not only directly, but also by affecting emotional eating; therefore, adolescent girls should be taught how to cope with negative emotions using strategies other than increasing food consumption in response to negative emotions. Further studies should assess the mediating role of emotional eating among adolescent girls and evaluate the impact of stress management interventions on body weight. Full article

Metabolites, once viewed mainly as energy substrates or structural precursors, are now increasingly recognized as key extracellular signaling mediators that regulate diverse physiological processes. This review synthesizes and systematizes current knowledge on metabolite-mediated signaling through G-protein-coupled receptors (GPCRs) in teleosts and, importantly, highlights new conceptual links between specific metabolite–GPCR axes and key physiological functions relevant to aquaculture. By integrating evidence across metabolite–GPCRs axes, including succinate–SUCNR1, aromatic amino acids (tryptophan and phenylalanine)–GPR142, basic amino acids (L-arginine)–GPRC6A, and lactate–GPR81. We clarify how metabolite–receptor interactions have the potential to modulate glucose homeostasis, immune responses, energy metabolism, and stress coping. A major contribution of this review is illustrating how metabolites act not only as nutrients but also as extracellular signaling molecules governing core physiological processes via GPCRs. Particularly from an evolutionary perspective, compared with peptide-activated GPCRs, metabolite-sensing GPCRs are relatively conserved across different species, suggesting that relevant findings from biomedical research could be translated to aquaculture applications. Therefore, understanding GPCR-mediated metabolite sensing provides a molecular foundation for improving nutrient formulation, developing functional feeds, and designing selective breeding strategies in precision aquaculture. Full article

Background/Objectives: Although implantable cardioverter defibrillators (ICDs) play a critical role in reducing the risk of sudden cardiac death, patients may report various physical and psychological symptoms during the implantation process. This study aimed to examine ICD patients’ retrospective reports of pre-implantation symptoms, their concurrent assessment of post-implantation symptom experiences, and the non-pharmacological methods they used to cope with these symptoms. Methods: A cross-sectional study was conducted with ICD patients who visited the arrhythmia clinic between May and August 2022. Data were collected using a questionnaire developed by the researchers and medical records. The study analyzed changes in symptoms reported by patients, individual coping methods used, the perceived effectiveness of these methods, and comparisons of methods used according to descriptive and clinical characteristics. Results: Patients reported a decrease in chest pain, palpitations, dizziness, syncope, and shortness of breath after ICD implantation (p < 0.001). However, they reported an increase in fatigue and anxiety levels (p < 0.001); no significant change was reported for insomnia (p = 0.473) and fear (p = 0.082). Furthermore, no significant difference was observed between patients who received shock therapy and those who did not in terms of changes in anxiety and fatigue levels, and the increase in anxiety was similar in both groups. The most frequently reported coping method among patients was praying, followed by drinking herbal tea and walking. A significant relationship was found between marital status and coping method preference, while no relationship was found with other descriptive and clinical characteristics. Conclusions: Although ICD implantation reduces cardiac symptoms, anxiety and fatigue continue to increase. Patients’ tendency to turn to cultural and spiritual coping methods such as prayer indicates that post-ICD care should be conducted with a holistic approach that also covers psychological and spiritual needs. Full article

Background/Objectives: Caring for dementia patients involves a significant emotional burden for family caregivers, who confront anticipatory grief (AG) processes that can negatively affect their health-related quality of life (HRQoL). This study examines the mediating role of resilience in the relationship between AG and HRQoL. Methods: A cross-sectional study was conducted with 144 family caregivers of people with dementia in the province of Alicante (Spain). Validated instruments were applied to measure AG (Caregiver Grief Scale), HRQoL (SF-12), and resilience (Brief Resilient Coping Scale). Descriptive analyses, Pearson correlations, and hierarchical regressions were used, as well as a mediation model based on Hayes’ PROCESS macro. Results: AG was negatively associated with resilience (r = −0.19, p = 0.025) and with both mental (r = −0.24, p = 0.004) and physical (r = −0.22, p = 0.009) components of HRQoL, whereas resilience was positively associated with mental HRQoL (r = 0.35, p < 0.001). In regression analyses, AG (B = −3.36, p = 0.006) and resilience (B = 1.16, p < 0.001) were significant predictors of mental HRQoL, explaining 30.4% of the variance (R² = 0.30). Mediation analyses showed a significant indirect effect of AG on mental HRQoL through resilience (B = −1.28, 95% bootstrapped CI [−2.31, −0.84]), indicating partial mediation. Conclusions: Although AG negatively impacts HRQoL, resilience emerges as a relevant protective resource, especially for caregiver mental health. Therefore, it is crucial to promote resilient coping strategies in interventions that target this vulnerable population. Full article

Background/Objectives: Thirst is a common and distressing symptom experienced by individuals undergoing hemodialysis. It can affect patients’ comfort, adherence to fluid restrictions, and overall quality of life. Understanding how patients perceive and cope with thirst is essential for developing evidence-based nursing interventions that enhance patient comfort and support adherence to care recommendations. Aim: To investigate the perceptions, experiences, and perceived factors related to thirst among hemodialysis patients using a mixed-methods design. Methods: This study adopted a convergent parallel mixed-method design. Quantitative data were obtained from 72 hemodialysis patients using the Thirst Discomfort Scale and a structured questionnaire. Qualitative data were collected through in-depth semi-structured interviews with 22 patients. Data were analysed using SPSS 23.0 for the quantitative phase and descriptive phenomenological analysis (Colaizzi’s method) for the qualitative phase to explore underlying perceptions and experiences. Results: Quantitative findings indicated substantial thirst discomfort, reflected by elevated Thirst Discomfort Scale and VAS scores. Qualitative findings highlighted persistent dry mouth, emotional distress, perceived loss of control, and coping strategies such as limiting fluids, distraction, oral rinsing, consuming cold items, and faith-based coping. On integration, qualitative narratives aligned with the high burden captured by quantitative scores, underscoring the multidimensional nature of thirst in hemodialysis. Conclusions: This study demonstrates that thirst in hemodialysis patients is a multidimensional experience encompassing physiological, psychological, and behavioural components. The findings highlight the need for individualised, holistic nursing approaches that extend beyond fluid restriction alone. Relevance to clinical practice: Nurses should adopt holistic approaches addressing emotional and spiritual dimensions of thirst management, providing individualised education and psychosocial support. Full article

Recent studies indicated a connection between trace amine-associated receptor 5 (TAAR5) and emotional behaviors related to anxiety and depression; however, the neurobiological basis of this link is still unclear. Using mutant TAAR5 knockout (TAAR5-KO) mice, we explored the consequences of receptor deletion on dopamine (DA) dynamics in the ventral striatum and stress-related behaviors. Voltammetric measurements of DA in the nucleus accumbens (NAc) coupled with electrical stimulation of the ventral tegmental area (VTA) revealed that mice lacking TAAR5 display a greater DA release, while its reuptake is not affected. Behaviorally, mutants were significantly less anxious in the elevated plus maze (EPM) and consumed more sucrose in comparison with wild-type (WT) controls. The new object recognition test (NOR) did not uncover a difference between these genotypes. During predator (rat) stress exposure, mutant and WT mice showed quite distinct responses versus the behavior observed in stressless conditions. Control animals demonstrated a substantial increase in “freezing” (a sign of passive coping), while “running” and “exploring” patterns (signs of active coping) were significantly extended in mice lacking TAAR5. Short-term consequences of stress were explored 24 h following the predator exposure. The absence of TAAR5 did not prevent or reduce stress-induced anxiety in the EPM. In fact, the level of anxiety in mutants reached that observed in control mice. Furthermore, activity in NOR was significantly decreased in mice lacking TAAR5 but not in WT animals. On the other hand, predator exposure resulted in impaired NOR in the WT control, whereas mutants’ performance was not altered. These findings indicate that TAAR5 deletion leads to significant DA imbalance, which might at least partly explain the better stress-coping strategy and other stress-induced behavioral consequences observed in mutant animals. Full article

Arboreal ants occupy a thermally dynamic environment, yet the mechanisms integrating nest architecture and worker behavior to maintain colony homeostasis remain understudied. We investigated the interplay among circadian rhythm, nest homeostasis, and worker morphology in Azteca chartifex spiriti, a Neotropical arboreal species that builds large polydomous nests suspended in trees. In ten colonies, we measured internal moisture and temperature gradients in the main nest, which houses most individuals, including the reproductive female, immatures, and numerous workers. In six colonies, we assessed the polymorphism of foraging workers over a 24 h cycle in relation to external temperature variation. The results show integrated thermoregulatory mechanisms that combine passive strategies, derived from nest architecture and moisture gradients from the suspension base to the lower extremity, with active strategies linked to foraging patterns and worker polymorphism. Internal temperature (27.8 ± 2.41 °C) remained buffered relative to external fluctuations, and moisture was significantly higher at the nest’s lower extremity (p < 0.001). Worker size displayed a bimodal distribution during the day that shifted to a unimodal pattern at night, indicating behavioral adjustments to thermal and operational demands. These findings demonstrate that the interaction between physical structure and worker behavior maintains colony homeostasis, providing essential insights into how dominant canopy ants may cope with future climate change scenarios. Full article

Background/Objectives: Sepsis survivors frequently experience long-term complications known as Post-Sepsis Syndrome. Many survivors and their relatives express ongoing dissatisfaction with the quality and accessibility of health information. Yet the specific informational needs and preferred formats remain insufficiently defined. To identify the informational needs of sepsis survivors and their relatives across different stages of illness and recovery. Methods: This qualitative study, conducted within the AVENIR project, included semi-structured telephone interviews with 12 survivors and 6 relatives in Germany. Interviews were transcribed verbatim and analyzed using qualitative content analysis according to Mayring. Results: Eighteen interviews highlighted phase-specific gaps in information. Relatives reported urgent needs for timely, comprehensible and empathetic communication during the ICU phase, often while under decision pressure. Survivors described limited capacity to process information during the acute phase and sought orientation only after cognitive and emotional stabilization. After discharge, both groups reported an “information vacuum”, marked by insufficient guidance on long-term physical and psychological consequences, rehabilitation, vaccination, and follow-up care. Many participants received no informational material, or only general or inconsistent information. Desired content emphasized basic sepsis knowledge, explanations of persistent symptoms, practical coping strategies, and navigation of support services. Preferred formats included peer support and repeated, personal conversations with healthcare professionals, complemented by trusted online and printed resources. Conclusions: Sepsis survivors and relatives experience notable, role- and phase-specific information deficits that extend from the ICU into long-term recovery. Timely, reliable, and accessible information may help reduce uncertainty, support coping, and strengthen autonomy for both survivors and relatives. Full article

Climate change and global warming are deeply impacting natural foraging dependent upon rain fall. To understand how xerophytes cope with these dramatic changes, comparative transcriptomic profiling of Atriplex halimus and Atriplex leucoclada was investigated under drought stress. The data revealed both shared and species-specific adaptive mechanisms. Differentially expressed genes (DEGs) clustered into major conserved gene families, including stress signaling, transcriptional regulation, antioxidant defense, metabolism, transport, and hormone signaling. In A. halimus, drought tolerance was characterized by strong transcriptional regulation, redox balance, and energy homeostasis, highlighted by the up-regulation of WRKY, MYB, and SET-domain transcription factors, calcium transporters, SnRK1 kinases, and stress-protective proteins such as HSPs and LEAs. On the other hand, A. leucoclada exhibited broader signaling flexibility and structural reinforcement through enrichment of MAPKs, CDPKs, 14-3-3 proteins, and cell wall-modifying enzymes (XTHs, expansins, chitinase-like proteins), as well as high expression of transporters and hormone-responsive genes. Such patterns indicated distinct drought adaptation strategies: A. halimus relied on rapid transcriptional and redox adjustments suited for fluctuating moisture regimes, while A. leucoclada employed multi-layered, constitutive defenses for persistent arid conditions. Together, these results elucidate complementary molecular strategies enabling ecological divergence and drought resilience among closely related halophytes. Full article

In practical engineering, large-scale wind power integration typically requires long-distance transmission lines to deliver power to load centers. The resulting weak sending-end systems lack support from synchronous power sources. Under extreme weather conditions, the rapid increase in active power output caused by high wind power generation may lead to voltage instability. In existing projects, a phenomenon of repeated voltage fluctuations has been observed under fault-free system conditions. This phenomenon is induced by the coupling of the characteristics of weak sending-end systems and low-voltage ride-through (LVRT) discrimination mechanisms, posing a serious threat to the safe and stable operation of power grids. However, most existing studies focus on the analysis of voltage instability mechanisms and the optimization of control strategies for single devices, with insufficient consideration given to voltage fluctuation suppression methods under the coordinated operation of wind power and energy storage systems. Based on the actual scenario of energy storage configuration in wind farms, this paper improves the traditional LVRT discrimination mechanism and develops a coordinated voltage ride-through control strategy for permanent magnet synchronous generator (PMSG) wind turbines and energy storage batteries. It can effectively cope with unconventional operating conditions, such as repeated voltage ride-through and deep voltage ride-through that may occur under extreme meteorological conditions, and improve the safe and stable operation capability of wind farms. Using a hardware-in-the-loop (HIL) test platform, the coordinated voltage ride-through control strategy is verified. The test results indicate that it effectively enhances the wind–storage system’s voltage ride-through reliability and suppresses repeated voltage fluctuations. Full article