Search Results (10,394)

Fructus Ligustri Lucidi (FLL) is a traditional tonic Chinese medicine with a long history of application due to its various pharmacological effects, but there are fewer studies on the use of FLL to alleviate fatty liver in animals. The objective of the current study was to assess the influence of FLL fermentation products on fatty liver hemorrhagic syndrome in hens during the late stages of egg production. The study involved 288 Hy-Line Brown laying hens, aged 500 days, which were randomly allocated into 4 groups, with each group having 6 replicates of 12 chickens. Hens were fed a basal diet (control group, group Ι), a high-energy diet (model group, group II) and a high-energy diet supplemented with 0.05% and 0.1% FLL fermentation product (groups III and IV). The duration of the pre-experiment was 1 week, and the trial period was 12 weeks. The results showed that the addition of FLL fermentation product to high-energy diets significantly increased egg weight and reduced eggshell breakage rate. High-energy diet supplementation with FLL fermentation product increased serum T-AOC and T-SOD activity (p < 0.05), and reduced serum MDA content (p < 0.05). As more FLL fermentation product was added, there was a notable reduction in lipid droplets in liver tissues, alleviation of symptoms of steatosis, and suppression of mRNA expression levels of lipid synthesis genes ACC, FAS, and SCD in the liver (p < 0.001). Transcription factors such as NR1H3 (p < 0.01), LPK (p < 0.001), and SREBF1 (p < 0.05) were differentially down-regulated, significantly reducing MTTP gene expression (p < 0.001). It can be concluded that the addition of 0.1% FLL fermentation product can enhance the productivity of laying hens during their late laying phase, increase the antioxidant capacity, inhibit the mRNA expression level of genes related to fat synthesis in the liver, reduce the accumulation of fat, and alleviate the fatty liver of laying hens caused by the high-energy diet. Full article

Graphene oxide (GO) membranes present a compelling alternative to the permeability-selectivity trade-off inherent in conventional polymer membranes. However, the incomplete mechanistic understanding and the absence of scalable, defect-controlled fabrication processes continue to hinder their practical deployment. This review synthesizes and integrates transport mechanisms, computational modeling, fabrication, and translational constraints across graphene-based membrane architectures into a comprehensive design-oriented framework. Five key aspects of this synthesis are highlighted. Firstly, the available evidence supports a three-regime transport model, which unifies viscous near-frictionless flow, activated molecular hopping, and solution–diffusion. This reframes selectivity as a tunable function of the C/O ratio and interlayer chemistry. Secondly, a quantitative parity analysis of literature data reveals that classical molecular dynamics tends to overestimate GO laminate water permeance by a representative factor of approximately 3–8× across the matched comparisons examined. This discrepancy can be corrected using a tortuosity–porosity factor derived from wet-state XRD. Machine-learning force fields (GAP, MACE), while still in an early stage of development with limited reported applications, narrow the residual discrepancy to within 1.5–2× in the studies reviewed. Thirdly, a tiered computational roadmap identifies nuclear quantum effects as critical for proton-transport applications but unresolved for water permeance in GO laminate geometry. Fourthly, performance across water nanofiltration, gas separation, ion recovery, and osmotic energy harvesting is benchmarked against commercial references, with explicit caveats regarding the heterogeneity of testing conditions across cited studies, alongside a technology readiness assessment. Lastly, a standardized 500-h hydraulic stability protocol is proposed to facilitate cross-laboratory comparison. Collectively, this synthesis provides a structured, albeit not exhaustively validated, basis for the discussion of next-generation membrane design. Full article

Calcite habit engineering offers a route to transform CO₂ mineralization from bulk sequestration into value-added nanomaterial production. Here, we demonstrate that additive chemistry and seeding strategy can serve as separable, recipe-level levers for directing calcite habit formation within a unified CaO/Ca(OH)₂ gas–liquid carbonation platform. This strategy highlights how solution-mediated habit control can bridge fundamental calcite crystallization mechanisms with scalable CO₂ utilization and value-added carbonate nanomaterial production. Sodium glutamate yielded ~100 nm rhombohedral nanoparticles, staged MgSO₄/ZnSO₄ dosing produced whisker-like crystalline nanorods with aspect ratios of 4–7, and two-step seeded carbonation with NH₄Cl generated fusiform spindle subunits that assembled into hierarchical rosette architectures. X-ray diffraction confirmed calcite as the only crystalline calcium carbonate phase detected under the present measurement conditions, with no detectable aragonite or vaterite reflections. SEM/TEM revealed distinct primary-subunit architectures, including internal striations in spindle particles indicative of oriented attachment. Thermogravimetry, N₂ physisorption, and EDS further distinguished the products and showed that Mg/Zn/S modifiers in the whisker route are retained predominantly at crystal surfaces rather than incorporated into the calcite lattice. These results define calcite habit control through two independent levers: additive-driven facet selectivity and kinetic decoupling of nucleation from growth/assembly. The platform links scalable synthesis, CO₂ utilization, and functional carbonate design. Full article

Cooperative adaptive cruise control (CACC), as an extension of adaptive cruise control (ACC), is an intelligent transportation approach for connected and automated vehicles. By using vehicle-to-vehicle information, CACC improves longitudinal tracking performance, traffic throughput, and string-stable platoon behavior. However, controller tuning remains sensitive to vehicle-dynamics parameters, spacing-policy selection, fractional-order dynamics, and communication delay. This paper presents an analytical parameter-space-based fractional-order PD (FOPD) controller synthesis framework for string-stable CACC systems. For the constant-time headway spacing policy, the controller parameters are investigated in the $(k_p,k_d,\mu )$ parameter space, where the fractional differentiation order $\mu$ is considered as an additional design variable. To obtain the feasible stabilizing regions, the fractional-order characteristic equation is evaluated on the imaginary axis, and the delay-dependent stability boundaries are derived through a frequency-domain boundary-locus formulation. The stabilizing gain regions are constructed through the complex-root boundary (CRB), real-root boundary (RRB), and infinite-root boundary (IRB), which provide an interpretable graphical basis for controller-gain and fractional-order selection. In addition, the effect of the headway time on the admissible stability region is examined jointly with the fractional order. The proposed structure is implemented with a feedforward controller that uses the acceleration information of the preceding vehicle under a predecessor-vehicle-following communication topology. The selected fractional-order CACC (FO-CACC) controller is validated in an eight-vehicle platoon simulation environment and compared with integer-order ACC (IO-ACC), fractional-order ACC (FO-ACC), and integer-order CACC (IO-CACC) configurations. The results show that the proposed parameter-space approach enables systematic FOPD tuning and that the selected FO-CACC controller satisfies the frequency-domain string-stability requirement while maintaining smooth time-domain responses in position, velocity, acceleration, headway time, spacing error, and control input. Additional simulations under the New European Driving Cycle (NEDC) and the FTP-75 (Federal Test Procedure 1975) driving cycles further indicate that the proposed FO-CACC structure maintains accurate spacing regulation and bounded acceleration behavior under standard drive-cycle conditions. Overall, the results indicate that the fractional-order parameter provides an effective design freedom for improving string-stable cooperative platoon performance. Full article

The escalating threat of antimicrobial resistance has spurred extensive research into antimicrobial fibers. While numerous reviews have comprehensively cataloged the classification and mechanisms of natural, inorganic, and organic antimicrobial agents, a critical gap remains: few have systematically evaluated the engineering strategies that translate intrinsic biocidal activity into durable, real-world fiber performance. This review addresses this gap by shifting focus from encyclopedic enumeration to a problem-oriented critical assessment of performance optimization strategies. We examine recent advances in natural fibers (bamboo, hemp, chitosan, jute) and synthetic fibers modified with antimicrobial agents, with emphasis on three core challenges—poor wash durability of natural agents, aggregation and leaching of inorganic nanoparticles (e.g., Ag, ZnO, MOFs), and structural limitations of organic agents (e.g., QACs, QPSs, N-halamines, PHMB). Key optimization routes, including covalent grafting, microstructural control (e.g., triaxial microfluidic spinning), organic-inorganic hybridization, and rechargeable N-halamine systems, are critically assessed for their effectiveness in enhancing washing resistance, stability, and antimicrobial synergy. Based on this comparative synthesis, we identify future directions—smart-responsive systems, sustainable processing pathways, and standardized evaluation protocols—to guide the rational design of next-generation high-performance antimicrobial fibers. Full article

Background and Objectives: Dengue is emerging as a multifaceted public health challenge that extends beyond traditional vector-borne disease frameworks. Climate change, rapid urbanisation, environmental transformation, global mobility, and digital ecosystems are progressively reshaping transmission dynamics, outbreak patterns, and preparedness needs worldwide. This narrative review aimed to examine dengue from an integrated public health perspective, focusing on climate-sensitive transmission, urban health, surveillance and preparedness, digital epidemiology, artificial intelligence (AI), and health communication. Materials and Methods: A structured narrative review was conducted through targeted literature searches in PubMed, Scopus, and Web of Science between April and May 2026. To this end, a series of separate thematic search strategies were developed to explore the principal conceptual domains addressed in the review. The synthesis was organised around five interconnected preparedness domains: climate change and environmental transformation; urbanisation and urban health; surveillance, vaccination, and integrated preparedness; digital health, artificial intelligence, and mathematical modelling; and health communication and community engagement. The retrieved literature was analysed using a thematic narrative synthesis approach. Results: The retrieved evidence indicated the progressive expansion and redefinition of dengue risk across both endemic and historically non-endemic regions. Climate variability, environmental transformation, rapid urbanisation, and increasing human mobility have emerged as interconnected drivers capable of influencing vector ecology, transmission dynamics, outbreak frequency, and healthcare system vulnerability. Urbanisation has been frequently associated with infrastructural inequalities, environmental degradation, inadequate water and waste management, and territorial conditions favourable to vector proliferation. The extant literature has also placed significant emphasis on the growing importance of integrated surveillance systems and early warning approaches combining epidemiological, environmental, climatic, entomological, and mobility-related data. Digital epidemiology, AI-based predictive models, and digital surveillance tools may contribute to strengthening outbreak forecasting and preparedness capacity, although important limitations related to data quality, interoperability, interpretability, and implementation remain. In parallel, misinformation, risk communication challenges, and digital communication ecosystems emerged as relevant factors influencing public perception, preventive behaviours, institutional trust, and adherence to public health interventions. Conclusions: Dengue is a systems-level public health challenge shaped by climate change, urbanisation, environmental disruption, human mobility, health-system preparedness, and digital ecosystems. Conventional vector-control strategies alone are unlikely to adequately address this growing complexity. Strengthening dengue preparedness should therefore be considered a broader indicator of public health resilience and long-term health-system adaptation. Full article

Background/Objectives: Interest in alternative, non-animal sources of vitamin D has increased due to the global prevalence of its deficiency and the growing demand for plant-based dietary options. Mushrooms and algae have emerged as potential sustainable sources of vitamin D₂ and, in selected cases, vitamin D₃ following ultraviolet (UV) exposure. However, the comparative bioavailability and clinical effectiveness of vitamin D₂ relative to vitamin D₃ remain controversial. This review aims to evaluate the content of vitamin D₂ in mushrooms and algae, the impact of UV irradiation on its synthesis, and the effectiveness of vitamin D₂ supplementation compared with vitamin D₃ in humans. Methods: PubMed, ScienceDirect, and Google Scholar were searched (1996–2026). Only human intervention studies were included when assessing clinical efficacy. Data on natural sources and pharmaceutical formulations were analyzed. Results: UV irradiation markedly increases vitamin D₂ content in mushrooms, as compared to cultivated products. Algal vitamin D content varies, depending on species and UV exposure, with no robust clinical trials confirming improvement of serum 25(OH)D after algal supplementation. Across multiple randomized controlled trials, vitamin D₂ consistently increased circulating 25(OH)D₂ but frequently reduced 25(OH)D₃ and demonstrated lower efficacy in raising total 25(OH)D compared with vitamin D₃, as confirmed by a recent meta-analysis. Conclusions: Although UV-enhanced mushrooms represent a quantifiable dietary source of vitamin D₂, clinical evidence consistently indicates lower efficacy of vitamin D₂ compared with vitamin D₃. Algae cannot currently be considered a validated source of vitamin D for improving human vitamin D status. Further mechanistic and long-term clinical studies are required. Full article

The global proliferation of plastic waste has made the search for sustainable chemical recycling strategies imperative to transition toward a circular bioeconomy. This study presents a dual-valorization approach for polylactic acid (PLA) waste, utilizing it both as a sustainable precursor for g-C₃N₄ catalyst synthesis and as a sacrificial agent for green hydrogen production via photoreforming. Platinum-modified graphitic carbon nitride catalysts were synthesized and evaluated using pure lactic acid and commercial PLA waste under solar-simulated irradiation. Results identified C₃N₄-NaOH-Pt as the most active material, while the simultaneous one-pot depolymerization/photoreforming of macroscopic PLA fragments exhibited a peak H₂ production rate of 1.5 mmol·h⁻¹·g⁻¹, remarkably surpassing both the pure monomer model and pre-depolymerized solutions. This enhanced performance is tentatively attributed to a “controlled release” mechanism that prevents catalyst surface saturation and minimizes light scattering effects inherent to fine powders. The study concludes that maintaining the macroscopic integrity of PLA waste provides a strategic advantage for chemical reforming by eliminating energy-intensive grinding and pretreatment. Future research into diverse operational and chemical parameters, including temperature and base-addition strategies, will be essential for scaling solar-driven upcycling technologies. Full article

This paper presents the synthesis and characterization of Schiff base ligands (L) derived from NH-pyrazole-indole and their ruthenium(II) complexes of the general formula [Ru(p-cymene)(L)Cl₂]. The structural characterization of the ligands and ruthenium complexes was performed using a range of analytical methods, including infrared spectroscopy (IR), Raman spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, elemental analysis, mass spectrometry(ESI-MS), density functional theory (DFT) calculations, and single-crystal X-ray diffraction crystallography for two of the four synthesized compounds. Schiff base ligands (L) derived from NH-pyrazole-indole and their ruthenium(II) complexes were evaluated against antibiotic-resistant bacteria. The effect of substituting the ligand with methoxy groups on biological activity was assessed. The antibacterial activity, Minimal Inhibitory Concentration (MIC), and Minimal Bactericidal Concentration (MBC) of the free ligands and the ruthenium compounds were measured against six bacterial isolates (Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae) and two control strains (Enterococcus faecalis ATCC 29212 and Enterobacter cloacae ATCC 13047) from the ESKAPE group of highly antibiotic-resistant pathogens. The free ligands were inactive, whereas all four ruthenium complexes displayed notable antibacterial activity. In particular, compounds Ru3 and Ru4 exhibited higher activity against Staphylococcus aureus than the Trimethoprim control, with MIC values of 15.60 µg/mL, compared with an MIC > 64 µg/mL for Trimethoprim. The activity of those two complexes was similar to that of Gentamicin against this strain. Molecular docking calculations suggested a possible mechanism of action through binding of the complexes to the active site of S. aureus PBP2a. Full article

Background/Objectives: Activated irrigation techniques improve intracanal disinfection, but their impact on the clinical and radiographic healing of apical periodontitis remains unclear. This systematic review and meta-analysis evaluated the efficacy of passive ultrasonic irrigation (PUI) and laser-assisted irrigation (LAI), without adjunctive systemic antibiotics, on periapical healing compared with conventional irrigation in adult patients. Methods: Following PRISMA guidelines and prospective registration in PROSPERO (CRD420261413401), PubMed, Scopus, Embase, and Web of Science were searched up to February 2026. Randomized controlled trials (RCTs) involving adults with apical periodontitis comparing PUI or LAI against conventional syringe irrigation—with a minimum of 6 months follow-up—were included. Risk of bias was assessed using RoB 2, evidence certainty via GRADE, and a random-effects meta-analysis calculated pooled odds ratios (ORs) and 95% confidence intervals (CIs). Results: Of 1115 records identified, five randomized controlled trials involving 451 teeth fulfilled the eligibility criteria and were included in the qualitative synthesis and quantitative meta-analysis. Activated irrigation significantly increased periapical healing probability compared with conventional irrigation (OR = 2.25; 95% CI: 1.29–3.93; p = 0.004), with no statistical heterogeneity (I² = 0%). Subgroup analyses showed significant benefits for both PUI (OR = 1.95; 95% CI: 1.08–3.54) and LAI (OR = 6.77; 95% CI: 1.63–28.12). The overall certainty of evidence was moderate due to risk of bias concerns. Conclusions: Activated irrigation techniques (PUI and LAI) were significantly associated with improved clinical and radiographic healing of apical periodontitis compared with conventional irrigation alone. Enhanced intracanal disinfection contributes to a more predictable resolution of periapical lesions without adjunctive systemic antibiotics. Further high-quality RCTs with standardized protocols and long-term CBCT-based follow-up are required to confirm these findings. Full article

The governance of migrant and refugee health is increasingly shaped by political rationalities of risk, belonging, and control, yet critical analyses using Foucauldian notions remain fragmented across disciplines. This scoping review systematically maps how health interventions, services, and policies directed at migrant and refugee populations have been examined through the Foucauldian framework. Following PRISMA-ScR guidelines, MEDLINE (PubMed), Scopus, CINAHL (EBSCO), and APA PsycINFO were searched, identifying 270 records. After deduplication, 115 unique records were screened, and 56 full-text articles were assessed for eligibility. Thirty-seven studies (n = 37) met the inclusion criteria, spanning multiple countries across five continents and employing qualitative research methods. Thematic synthesis revealed four dominant analytic categories: (a) migration health as biopolitical strategy in neoliberal contexts; (b) health literacy as a technology of governmentality; (c) disciplinary and surveillance practices in migration health settings; and (d) resistance, counter-conduct, and emancipatory health practices. Findings indicate that while biopolitical and governmentality analyses predominate, later Foucauldian concepts—particularly care of the self and counter-conduct—remain underutilized. Health literacy is rarely theorized explicitly as governance, despite its frequent implicit deployment as a normalizing technology. This review advances theoretical application in migration health, identifies critical gaps in the literature, and offers a foundation for rethinking policies, professional practice, and advocacy with migrant populations. Full article

Background: Shoulder disorders are among the leading causes of musculoskeletal pain and disability worldwide. Despite the widespread use of corticosteroid injections, concerns regarding their short-term efficacy and potential adverse effects have encouraged research into alternative therapies such as oxygen–ozone therapy. This systematic review aimed to evaluate the efficacy of oxygen–ozone therapy in the management of shoulder disorders. Methods: A systematic search of PubMed, Scopus, and Web of Science was conducted up to 16 March 2026, according to PRISMA guidelines. Randomized controlled trials investigating localized oxygen–ozone injections for shoulder disorders were included. Pain scores and shoulder function were the main outcomes considered. Study quality was assessed using the Cochrane Risk of Bias 2 tool. Results: Five randomized controlled trials involving 263 participants with shoulder impingement syndrome, adhesive capsulitis, or chronic rotator cuff syndrome were included. Given the heterogeneity of the included studies, we only performed a qualitative synthesis. Oxygen–ozone therapy demonstrated significant improvement in pain and shoulder function across all of the included studies. Corticosteroid injections generally showed superior short-term pain relief, whereas oxygen–ozone therapy demonstrated more persistent clinical improvements during medium-to-long follow-up periods. Studies employing multiple ozone injection protocols reported more stable outcomes when compared with single-injection approaches. No major adverse events related to oxygen–ozone therapy were reported. However, substantial heterogeneity was observed regarding injection protocols, ozone concentrations, number of injections, and follow-up duration. Conclusions: Oxygen–ozone therapy appeared to be a potentially effective treatment option for shoulder disorders, particularly in patients with contraindications to corticosteroids or chronic degenerative conditions. While corticosteroid injection might provide short-term symptom relief, repeated oxygen–ozone injection protocols seemed to offer sustained clinical benefits over time. Further high-quality randomized controlled trials with standardized protocols and longer follow-ups are needed to better define the role of oxygen–ozone therapy in shoulder rehabilitation. Full article

Porous silicate scaffolds represent a promising class of grafting materials for hard tissue engineering due to their superior bioactivity, adjustable degradation rates, and ability to stimulate both osteogenesis and angiogenesis. In this work, scaffolds based on an akermanite-targeted (Ca₂MgSi₂O₇) starting composition, including copper-doped variants, were synthesized using sol–gel and combustion routes, followed by 3D printing to achieve porous architectures with controlled pore size and interconnectivity. The powders were characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and thermal analysis to evaluate their morphology, composition, and crystalline phases. The scaffolds were further assessed in terms of bioactivity by immersion in simulated body fluid (SBF), antibacterial activity, and in vitro cellular response. The results confirmed that copper doping enhanced antibacterial properties, while maintaining favorable biological behavior. Comparative analysis revealed differences between the two synthesis methods, with sol–gel providing more homogeneous structures and combustion leading to highly porous morphologies. These findings highlight copper-doped silicate scaffolds as promising candidates for bone tissue regeneration, combining architectural integrity with biological functionality. Full article

Foodborne pathogens rely on colonization, biofilm formation, virulence expression, and environmental adaptation as fundamental biological drivers of food safety risk. Quorum sensing (QS), a cell-density-dependent microbial communication mechanism, coordinates the expression of these key phenotypes by integrating intraspecies, interspecies, and host-derived signals, making QS an attractive intervention target in food microbial control. Although QS research has advanced considerably in recent years, existing reviews have largely focused on individual bacterial species or specific classes of signal molecules. A systematic integration of how QS coordinately drives both food spoilage and pathogen virulence remains lacking. In this review, we conceptualize the QS network as a central regulatory hub connecting microbial signal perception to hazardous phenotype expression. We systematically examine the mechanistic roles of QS in food spoilage, biofilm formation, host colonization and invasion, and toxin production. We also summarize current QS-targeted intervention strategies, including inhibition of signal synthesis, enzymatic signal degradation, receptor antagonism, and indirect regulation via beneficial microorganisms. Building on the available evidence, we further analyze the key challenges limiting practical application: signal system specificity, ecological safety, industrial-scale feasibility, and microbial adaptability. Overall, QS-based strategies offer a non-bactericidal route for food microbial control, although substantial barriers remain for translation into complex food matrices. Reframing QS function and intervention from the perspective of food safety risk formation provides an analytical framework that bridges mechanistic understanding with practical application. This framework also establishes a theoretical foundation for developing next-generation food preservation and foodborne disease control strategies. Full article

6-PPD quinone (6-PPDQ) has been recognized as a typical emergent contaminant with the potential to cause multiple aspects of damage to organisms. Creatine is an important metabolite that mediates energy homeostasis. In Caenorhabditis elegans, creatine content was reduced by 0.1–10 μg/L of 6-PPDQ, which was accompanied by a decrease in the expression of argk-1 encoding a creatine kinase and snf-5 encoding a potential creatine transporter. Creatine content could be reduced by RNAi of argk-1 and snf-5. Moreover, RNAi of argk-1 and snf-5 aggravated 6-PPDQ-induced immunosuppression, reflected by decreased expression of antimicrobial genes (lys-7 and spp-1), and double RNAi of argk-1 and snf-5 resulted in more severe immunosuppression induction in 6-PPDQ-exposed nematodes. After 6-PPDQ exposure, RNAi of argk-1 and snf-5 decreased the expression of aak-2 encoding AMPK, and aak-2 RNAi also strengthened 6-PPDQ-induced immunosuppression. During control of 6-PPDQ caused immunosuppression, ARGK-1, SNF-5, and AAK-2 modulated expressions of PMK-1/p38 MAPK and DAF-16 signals. The immunosuppression and inhibition in PMK-1 and DAF-16 expressions induced by 6-PPDQ could be further suppressed by creatine treatment. Therefore, the environmental exposure risk of 6-PPDQ in disrupting creatine synthesis and transporter was suggested, which potentially contributes to the induction of immunosuppression in nematodes. Full article