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Search Results (192)

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Keywords = contraction pair

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24 pages, 5313 KiB  
Article
The Influence of Gravity Gradient on the Inertialess Stratified Flow and Vortex Structure over an Obstacle in a Narrow Channel
by Karanvir Singh Grewal, Roger E. Khayat and Kelly A. Ogden
Fluids 2025, 10(8), 195; https://doi.org/10.3390/fluids10080195 - 29 Jul 2025
Viewed by 169
Abstract
The current study examines the influence of a varying gravity field and its interaction with density stratification. This represents a novel area in baroclinic flow analysis. The classical vortex and internal wave structures in stratified flows are shown to be significantly modified when [...] Read more.
The current study examines the influence of a varying gravity field and its interaction with density stratification. This represents a novel area in baroclinic flow analysis. The classical vortex and internal wave structures in stratified flows are shown to be significantly modified when gravity varies with height. Vortices may shift, stretch, or weaken depending on the direction and strength of gravity variation, and internal waves develop asymmetries or damping that are not present under constant gravity. We examine the influence of gravity variation on the flow of both homogeneous and density-stratified fluids in a channel with topography consisting of a Gaussian obstacle lying at the bottom of the channel. The flow is without inertia, induced by the translation of the top plate. Both the density and gravity are assumed to vary linearly with height, with the minimum density at the moving top plate. The narrow-gap approach is used to generate the flow field in terms of the pressure gradient along the top plate, which, in turn, is obtained in terms of the bottom topography and the three parameters of the problem, namely, the Froude number and the density and gravity gradients. The resulting stream function is a fifth-order polynomial in the vertical coordinate. In the absence of stratification, the flow is smooth, affected rather slightly by the variable topography, with an essentially linear drop in the pressure induced by the contraction. For a weak stratified fluid, the streamlines become distorted in the form of standing gravity waves. For a stronger stratification, separation occurs, and a pair of vortices generally appears on the two sides of the obstacle, the size of which depends strongly on the flow parameters. The influence of gravity stratification is closely coupled to that of density. We examine conditions where the coupling impacts the pressure and the velocity fields, particularly the onset of gravity waves and vortex flow. Only a mild density gradient is needed for flow separation to occur. The influence of the amplitude and width of the obstacle is also investigated. Full article
(This article belongs to the Section Geophysical and Environmental Fluid Mechanics)
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12 pages, 1122 KiB  
Article
Neuromuscular Strategies in Novice and Advanced Taekwondo Athletes During Consecutive Roundhouse Kicks
by Mauricio Barramuño-Medina, Pablo Aravena-Sagardia, Pablo Valdés-Badilla, Jordan Hernandez-Martinez, Tomás Espinoza-Palavicino, Cristian Sandoval and Germán Gálvez-García
Appl. Sci. 2025, 15(15), 8356; https://doi.org/10.3390/app15158356 - 27 Jul 2025
Viewed by 213
Abstract
Background: This study investigates differences in muscle co-contraction and peak electromyography (EMG) activity between novice and advanced Taekwondo athletes during consecutive roundhouse (bandal chagui) kicks, examining the influence of body composition and experience level. Methods: Sixteen Taekwondo athletes (12 males, 4 [...] Read more.
Background: This study investigates differences in muscle co-contraction and peak electromyography (EMG) activity between novice and advanced Taekwondo athletes during consecutive roundhouse (bandal chagui) kicks, examining the influence of body composition and experience level. Methods: Sixteen Taekwondo athletes (12 males, 4 females; mean age: 20.5 ± 4.3 years) were divided into novice (n = 8) and advanced (n = 8) groups. Muscle co-contraction indices and peak EMG activity across 15 consecutive kicks were assessed in key lower limb muscles, including the biceps femoris (BF), lateral gastrocnemius (LG), rectus femoris (RF), soleus (SO), semitendinosus (ST), tibialis anterior (TA), vastus lateralis (VL), and vastus medialis (VM). Results: Advanced athletes exhibited significantly higher co-contraction indices in BF–RF, VM–BF, and SO–TA pairs (p < 0.05) and increased peak EMG trends in the BF and LG (p < 0.05). Novice athletes showed significantly reduced peak EMG increases in the RF, VM, and VL. EMG trends were influenced by body composition, with principal component analysis indicating that higher fat mass and lower muscle mass were associated with greater variations in muscle activation. Conclusions: These findings suggest that advanced athletes refine motor control through increased co-contraction, improving stability and efficiency, while novices exhibit less optimized coordination patterns. This study provides insights into the neuromechanical adaptations associated with expertise development in Taekwondo. Full article
(This article belongs to the Special Issue Application of Biomechanics in Sports Science)
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21 pages, 997 KiB  
Article
Explainable AI and Fuzzy Linguistic Interpretation for Enhanced Transparency in Public Procurement: Analyzing EU Tender Awards
by Cosmin Cernăzanu-Glăvan and Andrei-Ștefan Bulzan
Mathematics 2025, 13(13), 2215; https://doi.org/10.3390/math13132215 - 7 Jul 2025
Viewed by 278
Abstract
Despite the ideal of a unified Single Market, a powerful “home bias” pervades EU public procurement, hinting at unseen barriers that conventional analysis fails to capture. This study introduces an interpretable AI framework to investigate these dynamics, pairing a LightGBM model with SHapley [...] Read more.
Despite the ideal of a unified Single Market, a powerful “home bias” pervades EU public procurement, hinting at unseen barriers that conventional analysis fails to capture. This study introduces an interpretable AI framework to investigate these dynamics, pairing a LightGBM model with SHapley Additive exPlanations (SHAP) to examine the vast Tenders Electronic Daily (TED) database (2018–2023). Concretely, we propose a fuzzy linguistic layer that translates SHAP’s complex quantitative outputs into intuitive, human-readable terms. Our model effectively distinguishes local from non-local awards (AUC ≈ 0.855), revealing that while high-value contracts expectedly attract broader competition, the most potent predictors are a country’s own history of local awards and structural factors like the buyer’s type and location. This points not to isolated incidents, but, rather, to deep-seated patterns shaping market fairness. Our combined XAI-Fuzzy approach offers a new instrument for transparent governance, enabling policymakers to diagnose market realities and forge a more genuinely open and equitable European public square. Full article
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17 pages, 322 KiB  
Article
A New Class of (α,η,(Q,h),L)-Contractions in Triple Controlled Metric-Type Spaces with Application to Polynomial Sine-Type Equations
by Fatima M. Azmi
Axioms 2025, 14(7), 506; https://doi.org/10.3390/axioms14070506 - 27 Jun 2025
Viewed by 195
Abstract
This paper introduces a novel class of generalized contractions, termed (α,η,(Q,h),L)-contraction mapping, within the context of triple controlled metric-type spaces, extending the framework of fixed point theory in controlled structures. [...] Read more.
This paper introduces a novel class of generalized contractions, termed (α,η,(Q,h),L)-contraction mapping, within the context of triple controlled metric-type spaces, extending the framework of fixed point theory in controlled structures. The proposed mapping is defined using α-admissible and η-subadmissible functions, in conjunction with a control pair (Q,h) of upper class of type I, and incorporates Wardowski’s function L-contraction condition. Under suitable hypotheses, we establish both the existence and uniqueness of fixed points for this class of mappings. Several corollaries are derived as special cases of the main result. Moreover, we provide a nontrivial application by analyzing the solvability of a nonlinear equation involving powers of the sine function, thereby illustrating the utility of the developed theory. Full article
(This article belongs to the Section Mathematical Analysis)
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28 pages, 2868 KiB  
Article
Satellite-Based Seasonal Fingerprinting of Methane Emissions from Canadian Dairy Farms Using Sentinel-5P
by Padmanabhan Jagannathan Prajesh, Kaliaperumal Ragunath, Miriam Gordon and Suresh Neethirajan
Climate 2025, 13(7), 135; https://doi.org/10.3390/cli13070135 - 27 Jun 2025
Viewed by 472
Abstract
Methane (CH4) emissions from dairy farming represent a substantial yet under-quantified share of agricultural greenhouse gas emissions. This study provides an in-depth, satellite-based fingerprinting analysis of methane emissions from Canada’s dairy sector, using Sentinel-5P/TROPOMI data. We utilized a robust quasi-experimental design, [...] Read more.
Methane (CH4) emissions from dairy farming represent a substantial yet under-quantified share of agricultural greenhouse gas emissions. This study provides an in-depth, satellite-based fingerprinting analysis of methane emissions from Canada’s dairy sector, using Sentinel-5P/TROPOMI data. We utilized a robust quasi-experimental design, pairing 14 dairy-intensive zones with eight non-dairy reference regions, to analyze methane emissions from 2019 to 2024. A dynamic, region-specific baseline approach was implemented to remove temporal non-stationarity and isolate dairy-specific methane signals. Dairy regions exhibited consistently higher methane concentrations than reference areas, with an average methane anomaly of 17.4 ppb. However, this concentration gap between dairy and non-dairy regions notably narrowed by 57.23% (from 24.42 ppb in 2019 to 10.44 ppb in 2024), driven primarily by accelerated methane increases in non-dairy landscapes and a pronounced one-year contraction during 2022–2023 (−39.29%). Nationally, atmospheric methane levels rose by 3.83%, revealing significant spatial heterogeneity across provinces. Notably, an inverse relationship between the initial methane concentrations in 2019 and subsequent growth rates emerged, indicating spatial convergence. The seasonal analysis uncovered consistent spring minima and fall–winter maxima across regions, reflecting the combined effects of seasonal livestock management practices, atmospheric transport dynamics, and biogeochemical processes. The diminishing dairy methane anomaly suggests complex interplay of intensifying background methane emissions from climate-driven wetland fluxes, increasing fossil fuel extraction activities, and diffuse agricultural emissions. These findings underscore the emerging challenges in attributing sector-specific methane emissions accurately from satellite observations, highlighting both the capabilities and limitations of current satellite monitoring approaches. Full article
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22 pages, 1476 KiB  
Article
Wearable Ultrasound-Imaging-Based Visual Feedback (UVF) Training for Ankle Rehabilitation of Chronic Stroke Survivors: A Proof-of-Concept Randomized Crossover Study
by Yu-Yan Luo, Chen Huang, Zhen Song, Vaheh Nazari, Arnold Yu-Lok Wong, Lin Yang, Mingjie Dong, Mingming Zhang, Yong-Ping Zheng, Amy Siu-Ngor Fu and Christina Zong-Hao Ma
Biosensors 2025, 15(6), 365; https://doi.org/10.3390/bios15060365 - 6 Jun 2025
Viewed by 585
Abstract
This study investigated the effect of wearable ultrasound-imaging-based visual feedback (UVF) on assisting paretic ankle dorsiflexion training of chronic stroke survivors. Thirty-three participants with unilateral hemiplegia performed maximal isometric contractions on an isokinetic dynamometer in randomized conditions with and without UVF that provided [...] Read more.
This study investigated the effect of wearable ultrasound-imaging-based visual feedback (UVF) on assisting paretic ankle dorsiflexion training of chronic stroke survivors. Thirty-three participants with unilateral hemiplegia performed maximal isometric contractions on an isokinetic dynamometer in randomized conditions with and without UVF that provided by a wearable ultrasound imaging system. Torque parameters (mean, peak, percentage of maximal voluntary contraction) and tibialis anterior muscle thickness were analyzed across different contraction phases. Statistical comparisons were conducted using paired t-tests or Wilcoxon tests. Correlation analyses were performed using Pearson’s or Spearman’s tests. Results demonstrated that UVF significantly improved torque output, as evidence by the increased percentage of maximal voluntary contraction (%MVC) during entire contractions (p = 0.007), increased mean (p ≤ 0.022) and peak (p ≤ 0.044) torque and the %MVC (p ≤ 0.004) during mid and end phases, and larger muscle thickness during mid contraction (p = 0.045). Moderate correlations were found between torque and muscle thickness (r ≥ 0.30, p ≤ 0.049). These findings preliminarily supported the positive outcomes of real-time wearable UVFs in enhancing paretic ankle dorsiflexion strength and force control during isometric contractions in chronic stroke survivors. While the developed and validated new training protocol may potentially serve as a practical adjunct to existing rehabilitation approaches, further investigations emphasizing the functional outcomes and clinical translations are still needed to verify the clinical utility. Full article
(This article belongs to the Special Issue Innovative Biosensing Technologies for Sustainable Healthcare)
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19 pages, 332 KiB  
Article
Analytical Approach to Convergence, Stability, and Data-Dependence of Jungck-KF Iterative Scheme with Applications in Dynamic Market Equilibrium Analysis
by Khushdil Ahmad, Khurram Shabbir, Faraz Ali, Monica-Felicia Bota and Liliana Guran
Symmetry 2025, 17(6), 885; https://doi.org/10.3390/sym17060885 - 5 Jun 2025
Viewed by 378
Abstract
In this work, we employ a more thorough contractive condition to examine the stability and convergence behavior of an Jungck-type iterative scheme for a pair of non-self mappings in a Banach space. Our results show that this iterative scheme has a better rate [...] Read more.
In this work, we employ a more thorough contractive condition to examine the stability and convergence behavior of an Jungck-type iterative scheme for a pair of non-self mappings in a Banach space. Our results show that this iterative scheme has a better rate of convergence as compared to all existing Jungck-type iterative schemes. The norm of a Banach space is symmetric with respect to the origin. Symmetry can significantly influence both the theoretical underpinnings and practical convergence behavior of iterative schemes. Furthermore, we show the convergence behaviour of various Jungck-type iterative schemes with an Jungck-KF iterative scheme through an example. We also prove the data-dependence result for our proposed iterative scheme for non-self-mapping. Additionally, we provide an application of the Jungck-KF iterative scheme related to Dynamic Market Equilibrium. Full article
(This article belongs to the Special Issue Application of Symmetry in Equations)
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19 pages, 285 KiB  
Article
Extensions of Göhde and Kannan Fixed Point Theorems in Strictly Convex Banach Spaces
by Moosa Gabeleh and Maggie Aphane
Axioms 2025, 14(6), 400; https://doi.org/10.3390/axioms14060400 - 23 May 2025
Viewed by 363
Abstract
Let nonempty subsets E and F of a Banach space X be given, along with a mapping S:EFEF defined as noncyclic when S(E)E and S(F)F [...] Read more.
Let nonempty subsets E and F of a Banach space X be given, along with a mapping S:EFEF defined as noncyclic when S(E)E and S(F)F. In this case, an optimal pair of fixed points is defined as a point (p,q)E×F where p and q are fixed points of S that estimate the distance between E and F. This article explores an extended version of Göhde’s fixed point problem to identify optimal fixed point pairs for noncyclic relatively nonexpansive maps in strictly convex Banach spaces, while introducing new classes of noncyclic Kannan contractions, noncyclic relatively Kannan nonexpansive contractions using the proximal projection mapping defined on union of proximal pairs, and proving additional existence results with supporting examples. Full article
22 pages, 675 KiB  
Article
Effect of a Six-Month Dance Intervention on Postural Control and Fall-Related Outcomes in Older Adults with Mild Cognitive Impairment: A Randomized Controlled Trial
by Ulrich Thiel, Nicole Halfpaap, Berit K. Labott, Fabian Herold, Corinna Langhans, Kristinn Heinrichs, Patrick Müller, Notger G. Müller and Anita Hökelmann
Geriatrics 2025, 10(3), 67; https://doi.org/10.3390/geriatrics10030067 - 17 May 2025
Cited by 1 | Viewed by 809
Abstract
Background/Objectives: Older adults with mild cognitive impairment often exhibit reduced postural control and increased fall risk. As fall-related injuries consume substantial healthcare resources, the development of fall-preventive interventions is of public health relevance. This study aims to investigate the effects of a six-month [...] Read more.
Background/Objectives: Older adults with mild cognitive impairment often exhibit reduced postural control and increased fall risk. As fall-related injuries consume substantial healthcare resources, the development of fall-preventive interventions is of public health relevance. This study aims to investigate the effects of a six-month dance intervention on postural control and fall-related measures in older adults with mild cognitive impairment. Methods: In this randomized controlled trial, 55 participants were allocated to either an intervention group or a control group. The intervention group performed two 90-min dance training sessions per week for six months, while the control group maintained their usual activities of daily living. Postural control was operationalized via balance performance, which was measured with the Sensory Organization Test and the Limits of Stability Test. Neuromuscular function of the lower extremities was assessed via muscle contraction velocity using tensiomyography. Fear of falling was quantified with the Falls Efficacy Scale, and participants reported fall history over the past year. It was hypothesized that older adults with mild cognitive impairment participating in the six-month dance training would show significantly greater improvements in postural control and fall-related outcomes than those in the control group. Results: A mixed analysis of variance (time × group) revealed no significant improvements in balance performance or neuromuscular function following the dance intervention (p > 0.05). However, several main effects for time were observed in the Sensory Organization Test, Limits of Stability Test, and muscle contraction velocity. Scores on the Falls Efficacy Scale improved significantly in the intervention group, reflecting reduced fear of falling, although only shown by a paired-samples t-test (t(23)= 2.276, p = 0.032, d = 0.465). Conclusions: This study did not provide evidence that a six-month dance intervention improves postural or neuromuscular functions. However, it cannot be ruled out that such null findings are related to confounding factors, such as insufficient training specificity or duration. Nonetheless, the fear of falling was significantly reduced in the intervention group, suggesting potential benefits for perceived fall risk in older adults with mild cognitive impairment. Full article
(This article belongs to the Section Geriatric Public Health)
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22 pages, 2491 KiB  
Article
Decentralized Blockchain-Based Authentication and Interplanetary File System-Based Data Management Protocol for Internet of Things Using Ascon
by Hiba Belfqih and Abderrahim Abdellaoui
J. Cybersecur. Priv. 2025, 5(2), 16; https://doi.org/10.3390/jcp5020016 - 23 Apr 2025
Cited by 1 | Viewed by 1040
Abstract
The increasing interconnectivity of devices on the Internet of Things (IoT) introduces significant security challenges, particularly around authentication and data management. Traditional centralized approaches are not sufficient to address these risks, requiring more robust and decentralized solutions. This paper presents a decentralized authentication [...] Read more.
The increasing interconnectivity of devices on the Internet of Things (IoT) introduces significant security challenges, particularly around authentication and data management. Traditional centralized approaches are not sufficient to address these risks, requiring more robust and decentralized solutions. This paper presents a decentralized authentication protocol leveraging blockchain technology and the IPFS data management framework to provide secure and real-time communication between IoT devices. Using the Ethereum blockchain, smart contracts, elliptic curve cryptography, and ASCON encryption, the proposed protocol ensures the confidentiality, integrity, and availability of sensitive IoT data. The mutual authentication process involves the use of asymmetric key pairs, public key registration on the blockchain, and the Diffie–Hellman key exchange algorithm to establish a shared secret that, combined with a unique identifier, enables secure device verification. Additionally, IPFS is used for secure data storage, with the content identifier (CID) encrypted using ASCON and integrated into the blockchain for traceability and authentication. This integrated approach addresses current IoT security challenges and provides a solid foundation for future applications in decentralized IoT environments. Full article
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18 pages, 1365 KiB  
Article
Maximal Torque, Neuromuscular, and Potentiated Twitch Responses to Sustained Forearm Flexion Tasks Using Different Anchor Schemes
by Robert W. Smith, Jocelyn E. Arnett, Dolores G. Ortega, Trevor D. Roberts, Dona J. McCanlies, Richard J. Schmidt, Glen O. Johnson and Terry J. Housh
Physiologia 2025, 5(2), 15; https://doi.org/10.3390/physiologia5020015 - 23 Apr 2025
Viewed by 381
Abstract
Background/Objectives: Studies of the effects of anchor schemes (perceived intensity vs. relative intensity) on muscular performance have reported mixed findings. Therefore, the present study examined the effects of different anchor schemes on time-to-task failure (TTF), muscular performance, neuromuscular responses, and potentiated twitch torque [...] Read more.
Background/Objectives: Studies of the effects of anchor schemes (perceived intensity vs. relative intensity) on muscular performance have reported mixed findings. Therefore, the present study examined the effects of different anchor schemes on time-to-task failure (TTF), muscular performance, neuromuscular responses, and potentiated twitch torque (PTT). Methods: On separate days, 15 men (age = 21.5 ± 2.3 yrs) performed forearm flexion maximal voluntary isometric contractions (MVICs) before and after sustained tasks anchored to a rating of perceived exertion of 6 (RPEFT) and with the torque at RPE = 6 (TRQFT). Electromyographic amplitude (EMG AMP) and mean power frequency (EMG MPF) were recorded from the biceps brachii (BB). Supramaximal stimuli were delivered to the motor nerve of the BB following the MVICs to quantify the PTT. Repeated measures ANOVAs assessed the mean differences between anchor schemes for MVIC, neuromuscular, and PTT responses. Paired t-tests compared the magnitude of percent changes for the dependent variables. Results: The TTF for the RPEFT was longer (p < 0.001) than the TRQFT, but the MVIC decreased similarly (12.7 ± 9.5% vs. 20.3 ± 7.9%, p = 0.054). Electromyographic AMP did not change (p = 0.288), while EMG MPF decreased (15.7 ± 10.2%, p < 0.011) for the TRQFT only. Mean decreases in PTT were comparable for both tasks (p < 0.003), although the percent change was greater for the TRQFT (49.6 ± 16.1%, p < 0.001). Conclusions: The differences in TTF, but similar decreases in MVIC suggested that participants reached a sensory tolerance limit. Based on EMG MPF and PTT, the TRQFT caused greater peripheral perturbations to contractile function than the RPEFT. Full article
(This article belongs to the Special Issue Exercise Physiology and Biochemistry: 2nd Edition)
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27 pages, 365 KiB  
Article
Common φ-Fixed Point Results for S-Operator Pair in Symmetric M-Metric Spaces
by Sadia Farooq, Naeem Saleem, Maggie Aphane and Ali Althobaiti
Symmetry 2025, 17(2), 254; https://doi.org/10.3390/sym17020254 - 7 Feb 2025
Viewed by 752
Abstract
In this article, we define a new class of noncommuting self mappings known as the S-operator pair. Also, we provide the existence and uniqueness of common fixed point results involving the S-operator pair satisfying the [...] Read more.
In this article, we define a new class of noncommuting self mappings known as the S-operator pair. Also, we provide the existence and uniqueness of common fixed point results involving the S-operator pair satisfying the (F,φ,ψ,Z)-contractive condition in m-metric spaces, which unifies and generalizes most of the existing relevant fixed point theorems. Furthermore, the variables in the m-metric space are symmetric, which is significant for solving nonlinear problems in operator theory. In addition, examples are provided in order to illustrate the concepts and results presented herein. It has been demonstrated that the results can be applied to prove the existence of a solution to a system of integral equations, a nonlinear fractional differential equation and an ordinary differential equation for damped forced oscillations. Also, in the end, the satellite web coupling problem is solved. Full article
(This article belongs to the Special Issue New Trends in Fixed Point Theory with Emphasis on Symmetry)
13 pages, 284 KiB  
Article
Generalized Weak Contractions Involving a Pair of Auxiliary Functions via Locally Transitive Binary Relations and Applications to Boundary Value Problems
by Nidal H. E. Eljaneid, Esmail Alshaban, Adel Alatawi, Montaser Saudi Ali, Saud S. Alsharari and Faizan Ahmad Khan
Mathematics 2025, 13(1), 163; https://doi.org/10.3390/math13010163 - 5 Jan 2025
Viewed by 880
Abstract
The intent of this paper was to investigate the fixed-point results under relation-theoretic generalized weak contractivity condition employing a pair of auxiliary functions ϕ and ψ verifying appropriate properties. In proving our outcomes, we observed that the partial-ordered relation (even, transitive relation) adopted [...] Read more.
The intent of this paper was to investigate the fixed-point results under relation-theoretic generalized weak contractivity condition employing a pair of auxiliary functions ϕ and ψ verifying appropriate properties. In proving our outcomes, we observed that the partial-ordered relation (even, transitive relation) adopted by earlier authors can be weakened to the extent of a locally ϝ-transitive binary relation. The findings proved herewith generalize, extend, improve, and unify a number of existing outcomes. To validate of our findings, we offer a number of illustrative examples. Our outcomes assist us to figure out the existence and uniqueness of solutions to a boundary value problem. Full article
19 pages, 2461 KiB  
Article
Optimization of Breeding Tools in Quinoa (Chenopodium quinoa) and Identification of Suitable Breeding Material for NW Europe
by Tim Vleugels, Chris Van Waes, Ellen De Keyser and Gerda Cnops
Plants 2025, 14(1), 3; https://doi.org/10.3390/plants14010003 - 24 Dec 2024
Viewed by 1093
Abstract
Quinoa (Chenopodium quinoa) cultivation has become increasingly popular in NW Europe but little is known about the performance of contract-free varieties in this region. In this study, we phenotyped 25 quinoa varieties on a single-plant basis in a field trial in [...] Read more.
Quinoa (Chenopodium quinoa) cultivation has become increasingly popular in NW Europe but little is known about the performance of contract-free varieties in this region. In this study, we phenotyped 25 quinoa varieties on a single-plant basis in a field trial in Belgium. In addition, we optimized breeding tools such as NIRS (near-infrared reflectance spectroscopy) to estimate the seed crude protein content and a multiplex PCR set to identify true F1 progeny from pair crosses. We identified 14 varieties with sufficiently early maturity, 17 varieties with plant height below 150 cm, 21 large-seeded varieties, four varieties with a crude protein content exceeding 15%, and two low-saponin varieties. A variety of seed colors and plant morphological traits was observed. Seed yield was not correlated with maturity, plant height or saponin content, but was negatively correlated with seed crude protein content. NIRS could accurately predict seed crude protein content with a determination coefficient of 0.94. Our multiplex SSR set could correctly identify the paternity in 77% to 97% of progeny, depending on the pair cross. In conclusion, our study identified various contract-free varieties that may be suitable for cultivation in NW Europe. In addition, our study provides valuable phenotypic information and breeding tools that breeders can harness for breeding efforts in NW European quinoa. Full article
(This article belongs to the Special Issue Genomics-Assisted Improvement of Quinoa)
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18 pages, 6964 KiB  
Article
Leveraging Synthetic Virology for the Rapid Engineering of Vesicular Stomatitis Virus (VSV)
by Chad M. Moles, Rupsa Basu, Peter Weijmarshausen, Brenda Ho, Manal Farhat, Taylor Flaat and Bruce F. Smith
Viruses 2024, 16(10), 1641; https://doi.org/10.3390/v16101641 - 21 Oct 2024
Viewed by 3767
Abstract
Vesicular stomatitis virus (VSV) is a prototype RNA virus that has been instrumental in advancing our understanding of viral molecular biology and has applications in vaccine development, cancer therapy, antiviral screening, and more. Current VSV genome plasmids for purchase or contract virus services [...] Read more.
Vesicular stomatitis virus (VSV) is a prototype RNA virus that has been instrumental in advancing our understanding of viral molecular biology and has applications in vaccine development, cancer therapy, antiviral screening, and more. Current VSV genome plasmids for purchase or contract virus services provide limited options for modification, restricted to predefined cloning sites and insert locations. Improved methods and tools to engineer VSV will unlock further insights into long-standing virology questions and new opportunities for innovative therapies. Here, we report the design and construction of a full-length VSV genome. The 11,161 base pair synthetic VSV (synVSV) was assembled from four modularized DNA fragments. Following rescue and titration, phenotypic analysis showed no significant differences between natural and synthetic viruses. To demonstrate the utility of a synthetic virology platform, we then engineered VSV with a foreign glycoprotein, a common use case for studying viral entry and developing anti-virals. To show the freedom of design afforded by this platform, we then modified the genome of VSV by rearranging the gene order, switching the positions of VSV-P and VSV-M genes. This work represents a significant technical advance, providing a flexible, cost-efficient platform for the rapid construction of VSV genomes, facilitating the development of innovative therapies. Full article
(This article belongs to the Special Issue Vesicular Stomatitis Virus (VSV))
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