Search Results (12,130)

The purpose of this non-randomized study was to determine the effect of strength training and aerobic interval training on the anaerobic and aerobic power and endurance of young men (assessed by determination of the second ventilatory threshold (VT2)) in non-trained men. Participants (n = 45) were recruited into three groups of 15 each. The first group performed strength training (ST), the second performed aerobic interval training (AIT), and the third group was the control group (CON). In each group, somatic measurements and tests of aerobic (graded test with VT2 determination) and anaerobic capacity (Wingate test) were performed twice (before and after the exercise intervention in the training groups). In the graded test, the level of maximal load (Pmax), maximal oxygen uptake (VO₂max) and intensity and oxygen uptake at VT2 were determined. In the Wingate test, peak power (PP) and mean power (MP) were determined. The exercise intervention in the ST and AIT groups lasted 6 weeks, with three workouts per week. Training in the ST and AIT groups resulted in significant increase in absolute Pmax (p < 0.001, ES = 0.52 and p < 0.05, ES = 0.36), VO₂max (p < 0.001, ES = 0.50 and p = 0.02, ES = 0.55) in the participants. Only AIT was significantly effective in improving oxygen uptake at VT2 (p < 0.04, ES = 0.64), and ST in improving PP. Strength training can be an effective training method in training aerobic and anaerobic capacity (significantly increases Pmax, VO₂max, and PP), while it does not significantly affect work intensity at VT2. Our results suggest that, particularly in anaerobic–aerobic sports, strength training may be a training method that can simultaneously improve both anaerobic power and maximal oxygen uptake. It can also complement endurance training. Full article

In this article, daily CO₂ emissions for the years 2019–2022 are examined using fractional integration for Brazil, China, EU-27 (and the UK), India, and the USA. According to the findings, all series exhibit long memory mean-reversion tendencies, with orders of integration ranging between 0.22 in the case of India (with white noise errors) and 0.70 for Brazil (under autocorrelated disturbances). Nevertheless, the differencing parameter estimates are all considerably below 1, which supports the theory of mean reversion and transient shocks. These results suggest the need for a greater intensification of green policies complemented with economic structural reforms to achieve the zero-emissions target by 2050. Full article

Short bowel syndrome (SBS) is characterized by insufficient intestinal length to support absorption causing malnutrition. The bowel adapts to SBS via intestinal dilation and delayed gastric emptying but still often requires long-term parenteral nutrition. Current surgical options to lengthen the bowel pose significant risks and often provide limited expansion. ‘Distraction enterogenesis’ has been proposed as a technique to induce intestinal lengthening for SBS. The deployment of the intestinal expansion sleeve (IES) device is hypothesized to result in significant intestinal lengthening in vivo. A Roux-en-Y was created in the jejunum of seven rats for isolated IES deployment. The IES was precontracted over a Bucatini noodle and inserted into the isolated roux limb. After 4 weeks of deployment, rats were sacrificed, Roux-en-Y length recorded, and histology analyzed. A paired t-test was performed to compare initial and final roux limb lengths and histopathological tissue remodeling. Intestinal distraction evaluated at 4 weeks post deployment of the IES resulted in a significant 30.2% elongation in roux limb length (43.6 ± 14.4 mm to 56.4 ± 20.8 mm (p = 0.043, n = 7). IES samples showed changes in mucosal and submucosal integrity and bowel wall thickness in response to IES lengthening. In samples with partial mucosal erosion, the basal/regenerative layers of the mucosa were preserved. Distraction enterogenesis with significant intestinal lengthening in vivo has been achieved with the IES device. Histologic changes suggest all bowel functional layers and attributes are maintained through distraction enterogenesis. Future constructs of the IES may benefit from the addition of immunomodulators. Increasing intestinal mass with these devices may complement the treatment paradigm for SBS. Full article

Groundwater contamination by nitrates (NO₃⁻) and nitrites (NO₂⁻) is an urgent problem in rural areas of Eastern Europe, with profound public health and sustainability implications. This paper presents an integrated assessment of groundwater vulnerability and water quality in rural wells in the Ceanu Mare commune, Cluj County, Romania—a representative area of the Northern Transylvania Basin, characterized by diverse geological structures, intensive agricultural activities, and incomplete public water infrastructure. This study combines detailed hydrochemical analyses, household-level studies, and geological context to identify and quantify key factors influencing nitrate and microbial contamination in rural wells, providing a comprehensive perspective on water quality challenges in the central part of Romania. This study adopts a multidisciplinary approach, integrating detailed geotechnical investigations conducted through four strategically located boreholes. These are complemented by extensive hydrogeological and lithological characterization, as well as rigorous chemical and microbiological analyses of nearby wells. The results reveal persistently elevated concentrations of NO₃⁻ and NO₂⁻, commonly associated with inadequate livestock waste management and the proximity of manure storage areas. Microbiological contamination was also frequent. In this study, the NO₃⁻ levels in well water ranged from 39.7 to 48 mg/L, reaching up to 96% of the EU/WHO threshold (50 mg/L), while the NO₂⁻ concentrations varied from 0.50 to 0.69 mg/L, exceeding the legal limit (0.5 mg/L) in 87% of the sampled wells. Ammonium (NH₄⁺) was detected (0.25–0.34 mg/L) in all the wells, below the maximum allowed limit (0.5 mg/L) but indicative of ongoing organic pollution. All the well water samples were non-compliant for microbiological parameters, with E. coli detected in 100% of cases (5–13 CFU/100 mL). The regional clay–marl substrate offers only limited natural protection against pollutant infiltration, primarily due to lithological heterogeneity and discontinuities observed within the clay–marl layers in the study area. This research delivers a replicable model for rural groundwater assessment and addresses a critical gap in regional and European water safety studies. It also provides actionable recommendations for sustainable groundwater management, infrastructure development, and community risk reduction in line with EU water directives. Full article

Engaging in nightlife activities is an essential form of leisure, especially among young people. However, it also presents considerable risks associated with the occurrence of various forms of violence. Recently, increasing attention has been directed toward sexual violence occurring in nightlife settings. Despite numerous preventive efforts, such as awareness raising, bystander intervention training, alcohol policies, and situational preventive measures, sexual violence remains a persistent issue in nightlife contexts. With the extended usage of smartphones, mobile apps represent a promising approach that complements sexual violence prevention. This article presents findings from a systematic literature review and an online overview of mobile apps aimed at the intervention and prevention of sexual violence, focusing on identifying features suitable for nightlife contexts. The literature review revealed limited academic resources addressing such mobile apps, with only six articles found across four databases. An online review highlighted a wide range of mobile apps that tackle violence. However, when it comes to sexual violence, only 18 apps explicitly focus on prevention or intervention. Although many apps address some aspects of the problem, we noticed a lack of a comprehensive approach that would target all relevant stakeholders. Based on the conducted mobile app’s feature assessment, we propose a set of functionalities that should be incorporated into a holistic mobile app for nightlife sexual violence prevention, which may complement existing educational, community-based, and structural prevention efforts. Full article

Rural areas around the world are increasingly dealing with energy and environmental challenges. These challenges are particularly acute in developing countries, where persistent reliance on traditional energy sources—such as wood fuel—intersects with concerns about forest conservation and energy sustainability. While wood fuel use is often portrayed as unsustainable, it is important to acknowledge that much of it remains ecologically viable and socially embedded. This study explores the role of rural entrepreneurs in shaping low-carbon transitions at the intersection of household energy practices and environmental stewardship. Fieldwork was carried out in four rural Zambian communities in 2016 and complemented by 2024 follow-up reports. It examines the connections between household energy choices, greenhouse gas emissions, and forest resource dynamics. Findings reveal that over 60% of rural households rely on charcoal for cooking, with associated emissions estimated between 80 and 150 kg CO₂ per household per month. Although this is significantly lower than the average per capita carbon footprint in industrialized countries, such emissions are primarily biogenic in nature. While rural communities contribute minimally to global climate change, their practices have significant local environmental consequences. This study draws attention to the structural constraints as well as emerging opportunities within Zambia’s rural energy economy. It positions rural entrepreneurs not merely as policy recipients but as active agents of innovation, environmental monitoring, and participatory resource governance. A model is proposed to support sustainable rural energy transitions by aligning forest management with context-sensitive emissions strategies. Full article

Background/Objectives: Hypopharyngeal squamous cell carcinoma (HPSCC) carries a poor prognosis, and reliable, inexpensive biomarkers are needed to refine risk-stratified treatment. The Glasgow Prognostic Score (GPS), integrating C-reactive protein and albumin, reflects systemic inflammation and nutritional status, but its prognostic utility in curative radiotherapy for HPSCC remains unclear. Methods: We retrospectively reviewed 98 consecutive patients with pathologically confirmed HPSCC who received definitive tomotherapy (70 Gy in 35 fractions) from June 2015 to February 2024 at a single tertiary center. Pretreatment GPS was classified as 0–2. Overall survival (OS) and progression-free survival (PFS) were assessed by Cox proportional hazards models, which evaluated associations between GPS and other clinical parameters. Results: Median age was 68 years (range 41–89); 92% were male. GPS distribution was 0 in 74 patients (76%), 1 in 18 (18%), and 2 in 6 (6%). After a median follow-up of 36.2 months, 3-year OS and PFS for the whole cohort were 78.7% and 51.7%, respectively. Patients with GPS 0 showed significantly higher 3-year OS than those with GPS 1–2 (83.6% vs. 62.2%; p = 0.023). On multivariate analysis, elevated GPS (1–2) remained an independent predictor of worse OS (hazard ratio [HR] 2.62, 95% CI 1.03–6.70; p = 0.044) alongside poor performance status and advanced stage. Conclusions: Pretreatment GPS independently stratifies overall survival in HPSCC patients undergoing curative radiotherapy, complementing established clinical factors. Because CRP and albumin are routinely available, GPS may assist in identifying high-risk patients who could benefit from intensified multidisciplinary treatment. Prospective multicenter studies are warranted to validate these findings. Full article

Diagnosing and prognosing immune-mediated airway diseases, like hypersensitivity pneumonitis (HP) and sarcoidosis, is complicated due to their overlapping symptoms and the lack of definitive biomarkers. Hence, we wanted to compare bronchoalveolar lavage (BAL) cytokine and chemokine profiles from 92 patients with different immune-mediated and inflammatory airway diseases, namely, HP, sarcoidosis, non-allergic asthma, amiodarone lung, and EGPA. We also compared pulmonary function parameters, BAL’s cellularity, and lymphocyte immunophenotypes. We found significant differences across all measured lung functions (VC, VC%, FEV1, FEV1%, and Tiff%) and in the number of macrophages, lymphocytes, neutrophils, and eosinophils. Furthermore, we showed significant differences in CD4, CD8, and CD4/8 across all included ILDs and OLDs; however, no significant differences were found in CD3, CD19, NK, or NKT. We identified nine biomarkers (IL-1β, IL-6, IL-8, IL-13, VEGF, angiogenin, C4a, RANTES, and MCP-1) that significantly differ in the BAL of patients with HP and sarcoidosis and showed that RANTES and IL-6 are associated with fibrotic outcome. We have demonstrated that interstitial and obstructive lung diseases differ in cytokine and cellular lung imprint, which may, in the future, enable the determination of the disease subtype and thus the identification of targets for the treatment of individuals or subgroups within diseases. Full article

This study examines the challenges that general education teachers face in implementing inclusive education for students with intellectual disabilities in Qatar. Employing a mixed-methods approach, quantitative data were collected from 255 teachers via a structured questionnaire, complemented by qualitative insights from semi-structured interviews with five participants. The findings indicate that teachers encounter moderate to high levels of difficulty, particularly in areas related to the school environment and the availability of educational resources. Significant demographic differences were observed, with male teachers and those working in primary schools reporting greater perceived barriers. Thematic analysis revealed six key factors influencing teachers’ perceptions: student diversity, instructional practices, teacher preparedness, school culture and environment, family and community involvement, and logistical challenges. Despite national policy support for inclusive education, the study reveals ongoing deficits in teacher training, institutional backing, and resource provision. These findings underscore the complex intersection of individual, institutional, and societal factors shaping inclusive education efforts. The study offers valuable insights for localizing inclusive education efforts in Qatar and similar contexts, with implications for educators, policymakers, and educational leaders committed to fostering equitable learning environments. Full article

The excessive use of antimicrobials drives the emergence of multidrug resistance (MDR) in bacterial strains, which harbor resistance genes to survive under diverse drug pressures. Such resistance can result in life-threatening infections. The predominance of MDR Pseudomonas spp. poses significant challenges to public health and environmental sustainability, particularly in ecosystems affected by human activities. Characterizing MDR Pseudomonas spp. is crucial for developing effective diagnostic tools and biosecurity protocols, with broader implications for managing other pathogenic bacteria. Strains were diagnosed through 16S rRNA PCR and sequencing, complemented by phylogenetic analysis to evaluate local and global evolutionary connections. Antibiotic susceptibility tests revealed extensive resistance across multiple classes, with MIC values surpassing clinical breakpoints. This study examined the genetic diversity, resistance potential, and phylogenetic relationships among Pseudomonas aeruginosa strain DG2 and Pseudomonas fluorescens strain FM3, which were isolated from solid waste dump sites (n = 30) and dairy farms (n = 22) in West Bengal, India. Phylogenetic analysis reveals distinct clusters that highlight significant geographic linkages and genetic variability among the strains. Significant biofilm production under antibiotic exposure markedly increased resistance levels. RAPD-PCR profiling revealed substantial genetic diversity among the isolates, indicating variations in their genetic makeup. In contrast, SDS-PAGE analysis provided insights into the protein expression patterns that are activated by stress, which are closely linked to MDR. This dual approach offers a clearer perspective on their adaptive responses to environmental stressors. This study underscores the need for vigilant monitoring of MDR Pseudomonas spp. in anthropogenically impacted environments to mitigate risks to human and animal health. Surveillance strategies combining phenotypic and molecular approaches are essential to assess the risks posed by resilient pathogens. Solid waste and dairy farm ecosystems emerge as critical reservoirs for the evolution and dissemination of MDR Pseudomonas spp. Full article

Background/Objectives: Neuropilin-1 (NRP1) is a key co-receptor for SARS-CoV-2, complementing the ACE2 receptor. Several investigations have documented highly conserved sequences in this receptor, supporting the implication of NRP1 as a key mediator in SARS-CoV-2 cellular entry mechanisms. Methods: To investigate this hypothesis, we examined 104,737 SARS-CoV-2 genome fastas from GISAID genomic data, corresponding to isolates collected between 2020 and 2025 in Mexico. Specifically, we focused on the RRAR motif, a known furin-binding site for NRP-1 and the binding site for ACE2 with the spike protein. Our analysis revealed high conservation (>98%) of the RRAR domain compared to a rapidly diminishing ACE2-binding domain. A complementary analysis, using Data from Gene Expression Omnibus (GEO, GSE150316), showed that NRP1 expression in lung tissue remains relatively stable, whereas ACE2 displayed high inter-individual variability and lower abundance compared to NRP1. Based on this evidence, we designed two humans–rats NRP1 siRNAs that were tested in vivo using a melittin-induced lung injury model. Results: The RT-PCR assays confirmed an effective NRP1 knockdown, and the siRNA-treated group showed a significant reduction in the lesions severity. These findings highlight NRP1 as a stable and relevant therapeutic target and suggest the protective potential of siRNA-mediated gene silencing. Conclusions: The evidence presented here supports the rational design of NRP1-directed therapies for multiple circulating SARS-CoV-2 variants in Mexico. Full article

Uniquely rooted in regenerative leadership and complemented by Complexity Leadership Theory (CLT), this conceptual essay offers a theoretical exploration of how educational institutions can act as dynamic systems that catalyze adaptive, community-led responses to anthropocentric socio-environmental crises. Rather than sustaining existing structures, educational leadership for regeneration seeks to restore ecological balance and nurture emergent capacities for long-term resilience. Positioned as key sites of influence, educational institutions are explored as engines of innovation capable of mobilizing students, educators, and communities toward collective environmental action. CLT offers a valuable lens for understanding how leadership emerges from nonlinear, adaptive processes within schools, enabling the development of innovative, collaborative, and responsive strategies required for navigating complexity and leading planetary-positive change. Drawing on a synthesis of the recent global literature, this paper begins by outlining the need to go beyond sustainability in envisioning regenerative futures, followed by an introduction to regenerative principles. It then examines the current and evolving role of educational leadership, the relevance in enabling whole-institution transformation, and how this relates to regenerative practices. The theoretical frameworks of systems thinking and CLT are introduced before noting their application within regenerative educational leadership. The final sections identify implementation challenges and offer practical recommendations, including curriculum innovation, professional development, and youth-led advocacy, before concluding with a call for education as a vehicle for cultivating planetary-conscious citizens and systemic change. This work contributes a timely and theoretically grounded model for reimagining educational leadership in an era of global turbulence. Full article

Background and Objectives: Heart failure (HF) represents a major public health challenge worldwide, with rising prevalence, high morbidity and mortality rates, and substantial healthcare costs. Non-invasive telemonitoring has emerged as a promising adjunct in HF management, yet its clinical effectiveness remains unclear. Materials and Methods: In this systematic review, we summarize randomized controlled trials (RCTs) between 2004 and 2024 examining the efficacy of non-invasive telemonitoring on mortality, readmission, and quality of life (QoL) in HF. In addition, we characterize the heterogeneity of features of different telemonitoring interventions. Results: In total, 32 RCTs were included, comprising 13,294 participants. While some individual studies reported benefits, non-invasive telemonitoring demonstrated mixed effects on mortality, readmission rates, and QoL. The most common modality for interfacing with patients was by mobile application (53%), followed by web portals (22%), and stand-alone devices (19%). Periodic feedback (63%) was more common than continuous feedback (31%) or on-demand feedback (6%). Clinician reviews of patient telemonitoring data was event-triggered (44%) more commonly than based on a prespecified timeline (38%). In most designs (90%), patients played a passive role in telemonitoring. Conclusions: Non-invasive telemonitoring interventions for HF exhibited considerable variation in duration and system design and had a low rate of patient engagement. Future work should focus on identifying telemonitoring-responsive subgroups and refining telemonitoring strategies to complement traditional HF care. Full article

Essential oils (EOs) are widely recognized for their antifungal properties, but their efficacy against specific phytopathogenic fungi associated with banana (Musa paradisiaca) rot remains underexplored. This study aimed to evaluate the antifungal potential of EOs from Origanum vulgare, Salvia rosmarinus, Syzygium aromaticum, Thymus vulgaris, Cinnamomum verum, and Ocimum basilicum against five fungal species isolated from infected banana peels. Fungal isolates were obtained using PDA medium supplemented with chloramphenicol and were purified by weekly subculturing. Morphological and microscopic characterization was complemented by molecular identification based on ITS sequencing and phylogenetic reconstruction using Neighbor-Joining and UPGMA methods in MEGA v11. In vitro and ex vivo antifungal assays were performed at EO concentrations ranging from 200 to 1000 ppm. Thyme oil exhibited the strongest inhibitory effect, with complete growth suppression at 1000 ppm. Cinnamon and oregano also demonstrated effective inhibition at 600 ppm, while clove, rosemary, and basil were markedly less effective. Statistical analysis confirmed significant effects of EO type and concentration on fungal growth (p < 0.001). Molecular results showed strong phylogenetic support for isolate identification, with bootstrap values above 93% in most clades. These findings support the selective use of specific EOs as sustainable alternatives to synthetic fungicides in the postharvest management of banana diseases and provide a molecularly supported basis for their targeted application in integrated control strategies. Full article