Search Results (46)

Background/Objectives: Emerging viral zoonoses represent a growing threat to global public health, with most newly emerging infectious diseases originating from animal reservoirs. Recent outbreaks of monkeypox, Ebola virus disease, Marburg virus disease, Rift Valley fever, and avian influenza highlight the capacity of zoonotic viruses to cross species barriers, spread internationally, and generate substantial health, social, and economic consequences. This review examines the ecological, epidemiological, and biological determinants of viral zoonotic emergence and transmission, with particular emphasis on vaccination and outbreak prevention strategies. Methods: A structured narrative review was conducted using a predefined literature search strategy across major scientific databases. Peer-reviewed epidemiological, clinical, and public health publications published between January 2000 and February 2026 were screened and selected according to predefined relevance criteria. Results: The emergence of viral zoonoses is driven by complex interactions among animal reservoirs, environmental and climatic changes, human behavior, and viral adaptation. Although transmission pathways and clinical outcomes differ among pathogens, common determinants of spillover and outbreak amplification were identified. Current evidence supports the importance of integrated surveillance, genomic monitoring, vaccination strategies, and community engagement as key components of preparedness and response. Emerging preventive approaches targeting pathogen transmission, including transmission-blocking strategies and vector-associated microbiota interventions, may provide additional opportunities for disease control. Conclusions: Strengthening preparedness for emerging viral zoonoses requires coordinated One Health approaches integrating human, animal, and environmental health. Future priorities include the development of next-generation vaccines, expansion of digital and genomic surveillance systems, improved equitable access to vaccines, and innovative interventions aimed at reducing zoonotic spillover and interrupting pathogen transmission. Full article

Dengue fever, with a high proportion of asymptomatic infections, poses a major global public health challenge that traditional surveillance systems frequently underestimate. Wastewater-based epidemiology (WBE) has emerged as a promising approach to monitoring infectious diseases beyond enteric viruses. Dengue virus is shed in urine, feces, and saliva, providing a biological basis for wastewater detection alongside clinical surveillance. This systematic review and meta-analysis synthesize current evidence on dengue virus (DENV) detection in wastewater and evaluate methodological factors influencing detection success in WBE. A systematic literature search using selected databases and predetermined keywords, followed by eligibility screening, resulted in ten studies being included, covering community surveillance and experimental trials. DENV ribonucleic acids (RNA) were most consistently detected and enriched in wastewater solids, indicating this matrix as the most reliable for surveillance. Among concentration methods, ultrafiltration achieved the highest viral recovery efficiency, while reverse transcription digital polymerase chain reaction (RT-dPCR) demonstrated superior sensitivity and precision compared to those of reverse transcription quantitative polymerase chain reaction (RT-qPCR), particularly at low viral concentrations. Storage at −80 °C was critical for preserving RNA integrity. The meta-analysis yielded a pooled DENV positivity rate of 24% (95% CI: 20–28%) after exclusion of outliers. Overall, solid-phase analysis combined with RT-dPCR represents the most sensitive methodological approach across the included studies. Harmonized protocols are needed to support future translation of dengue WBE into community surveillance as current evidence mainly demonstrates methodological feasibility and provides a technical foundation for future public health integration. Therefore, further longitudinal and multi-site validation is required to establish its broader applicability for dengue surveillance. Full article

African swine fever (ASF) remains a major challenge for global animal disease prevention and control. Public sentiment during ASF, along with farmer and consumer behavior, are underexplored factors in ASF control. This study examines how public sentiment influences farmers’ risk perceptions and consumers’ behavioral responses, including consumption substitution intention and pork price expectations, and assesses the implications of these behaviors for disease control effectiveness. Using provincial panel data from China (June 2021–November 2022), sentiment analysis of 1.19 million Weibo posts, and a micro-level survey of 920 farmers, we combine panel regression, spatial econometric analysis, and micro-level behavioral evidence. Results show that public sentiment significantly elevates farmers’ risk perception, which may influence reporting decisions, marketing timing, and biosecurity investment, thereby increasing the complexity of surveillance and disease control. Sentiment intensifies substitution intentions and shapes pork price expectations, leading to reduced demand for formally marketed pork and potential shifts to lower-traceability or less-inspected channels. Spatial analysis indicates that the half-decay distance for amplifying ASF risk via sentiment is about 1300 km, providing parameters for cross-jurisdictional coordination. These findings support integrating socio-behavioral indicators into veterinary early warning systems and designing targeted disease risk communication under a broader One Health framework. Full article

The accelerating frequency of emerging infectious diseases (EIDs) in livestock poses a significant threat to global food security, as well as to animal and public health. While wastewater-based surveillance (WBS) has advanced significantly for human health surveillance, its application to livestock production systems remains fragmented and lacks standardization. This review synthesizes current evidence on livestock wastewater-based surveillance (L-WBS) as an early-warning sentinel for emerging viral pathogens, evaluating their dynamics, economic impacts, biosecurity measures, and One Health implications. Existing studies demonstrate that L-WBS effectively detects emerging viral pathogens in agricultural effluent, swine manure, and municipal wastewater systems serving livestock regions, frequently preceding clinical outbreak recognition. We further conceptualized a multifactorial framework linking environmental drivers such as climate and ecological disruption and agricultural intensification to pathogen emergence dynamics. Economic assessments show substantial direct losses (approximately US$ 950 per H5N1-infected dairy cow and US$ 25.9 billion in African swine fever virus (ASFV)-related damages across China) alongside indirect costs from biosecurity implementation, workforce disruption, and supply-chain instability. We recommend prioritizing methodological standardization through unified sampling and extraction protocols, integration of next-generation sequencing for genomic surveillance, and cross-sectoral policy frameworks to operationalize L-WBS as a global early-warning infrastructure for mitigating zoonotic spillover and livestock-dependent community resilience. Full article

Oropouche fever (OF), caused by Oropouche virus (OROV), has expanded beyond its Amazonian range into Minas Gerais (MG), Brazil, raising concern about transmission in extra-Amazonian Atlantic Forest landscapes. Critical gaps persist regarding Culicoides vector communities, anthropophily, and climate-sensitive transmission risk in these newly affected regions. We conducted targeted entomological surveys outbreak-driven by human OF cases, standardized across five MG communities using CDC light traps and Protected Human Attraction (PHA) to characterize Culicoides composition. Females of Culicoides underwent RT-qPCR for OROV (n = 819) and physiological assessment (n = 312). We developed an entomological alert framework that integrates blood-fed abundance, minimum infection rate (MIR) upper confidence bounds, and environmental drivers (i.e., mean temperature, relative humidity and precipitation) via generalized additive mixed models, which explained 68% of the variability in Culicoides abundance and the alert index across communities. We collected 1171 Culicoides individuals representing five species (C. leopoldoi, C. paraensis, C. pusillus, C. foxi, and C. limai). C. leopoldoi (79.1%) and C. paraensis (20.3%) were the predominant species; notably, C. paraensis is recognized as the primary vector of OROV in the Americas. C. paraensis was documented for the first time in all five outbreak areas and dominated PHA captures (90%), suggesting anthropophily. Although no specimens tested OROV-positive (consistent with expected field infection rates of 0.01–1%), MIR upper bounds reached 132/1000 in low-sample settings and humidity and temperature strongly modulated abundance. This operational baseline and alert index transform virologically negative, sparse surveillance data into prioritized targets for intensified sampling and vector control during early, low-prevalence phases, when containment of OROV’s extra-Amazonian spread is still achievable. Full article

Background: Rickettsioses disproportionately affect vulnerable populations and are frequently misdiagnosed as other febrile illnesses in Yucatan, the Mexican state with the greatest diversity of Rickettsia spp. Although significant seroprevalence has been reported in rural communities, the last population-based study was conducted over two decades ago, despite environmental and social changes that have likely increased transmission risk. This study aimed to estimate the seroprevalence of spotted fever group (SFG) and typhus group (TG) of Rickettsia in an endemic area of southeastern Mexico. Methods: A cross-sectional analytical study was conducted among 390 participants. Indirect immunofluorescence was used to detect IgG antibodies against SFG and TG of Rickettsia. Sociodemographic characteristics of participants, along with environmental and community-level variables from their regions of residence, were analyzed. Results: The overall seroprevalence of both Rickettsia groups was 31.2%. Higher maximum temperatures were associated with an increase in Rickettsia seroprevalence (PR = 4.18; 95% CI: 3.40–5.14), while higher population density was associated with a decrease in seroprevalence (PR = 0.97; 95% CI: 0.96–0.98). Conclusions: Rickettsia seroprevalence in Yucatan remains high and is shaped by both environmental and demographic factors. These findings highlight the need to strengthen surveillance and prevention strategies that integrate ecological and social determinants within a One Health framework. Full article

Increased international trade and tourist flows are key factors in the introduction of transboundary animal diseases such as African swine fever (ASF). Despite the availability of sufficiently detailed data on legal commercial trade, the movement of pork products intended for personal consumption is insufficiently reported and difficult to track when carried in passenger luggage, and may act as an introduction pathway. In this study, we analyze the risk of ASF reintroduction in Sardinia, an island that has recently achieved disease-free status after a long epidemic. An anonymous questionnaire on travelers’ awareness of ASF and their food transportation habits was administered at the main Sardinian airports between July and December 2025, and a total of 6525 responses were received. The results show a low level of knowledge regarding ASF, with approximately 95% of respondents stating they were unaware of the disease, while almost 10% of travelers reported having transported meat or other pork products, often originating from countries where the virus is circulating. Our investigation highlights how passenger flows, especially during the seasonal peak, could act as a vector for potential disease reintroduction through the transport of pork products. The results stress that the adoption of behavioral survey tools is essential to supplement traditional surveillance systems, highlighting the need to improve targeted communication strategies at both ports and airports in order to reduce the risk of virus reintroduction and protect regional livestock. Full article

Background: Dengue infection (DI) is a mosquito-borne arboviral disease primarily transmitted by infected female Aedes mosquitoes. In Bangladesh, DI poses a substantial public health challenge with recurrent outbreaks and rising incidence rates. This systematic review assesses the epidemiological characteristics of dengue infection in Bangladesh, focusing on demographic, clinical, and geographic trends. Objectives: To analyze dengue prevalence, demographic distribution, clinical symptoms, and serotype patterns in Bangladesh, with an emphasis on urban–rural disparities, gender differences, and serotype evolution. Methods: A systematic search was conducted across PubMed, Scopus, Web of Science, and Global Health (Ovid) databases, reviewing studies published from 2000 to 2024. Following PRISMA guidelines, 25 studies meeting eligibility criteria were selected. Data extraction and quality assessment were independently performed by three reviewers, ensuring methodological rigor. Results: Dengue incidence was higher in urban areas, mainly affecting males aged 20–34, with dengue virus serotype 3 (DENV-3) as the dominant serotype. Fever, headache, and joint pain were the most common symptoms, while severe cases often presented with respiratory and hemorrhagic complications. Acute symptoms like dyspnea and dehydration spread rapidly in densely populated areas. In rural areas, dengue showed a more endemic pattern, with persistent symptoms such as gastroenteritis and muscle pain. Conclusion: Dengue is now firmly endemic in Bangladesh, with clear geographic, demographic, and clinical differences. The dominance of DENV-3 and its association with more severe illness highlight the need for targeted and context specific interventions. Control efforts should prioritize vector management, public education, and continuous surveillance in urban areas, while strengthening community surveillance and primary healthcare in rural settings. Further research on rural transmission and the clinical impact of DENV-3 is essential to guide effective and tailored dengue control strategies. Full article

Aedes aegypti is the primary urban vector for several important arboviruses, including dengue, chikungunya, yellow fever, and Zika viruses. Traditional dengue virus (DENV) surveillance relies on passive reporting of human cases, which often underestimates transmission due to asymptomatic or unreported infections. This study evaluated the utility of monitoring DENV in Ae. aegypti mosquitoes to improve detection of local dengue transmission and inform vector control strategies during the 2024 dengue epidemic in Puerto Rico. Mosquito surveillance was conducted in 15 neighborhoods within the San Juan metropolitan area where confirmed dengue cases had been recently reported. Adult female Ae. aegypti were collected weekly using Autocidal Gravid Ovitraps (AGO traps) placed within a 200 m radius of index cases. Pools of 1–20 mosquitoes were tested for DENV RNA and serotype using RT-PCR. Surveillance continued for up to 91 days in study areas, depending on virus detection. A total of 29,354 female Ae. aegypti were collected, of which 29,211 females were pooled (1–20 specimens per pool) into 3878 pools and analyzed. DENV was detected in 49 pools across 11 neighborhoods, with serotypes DENV-1, DENV-2, and DENV-3 identified. Multiple serotypes were sometimes detected in mosquitoes from the same neighborhood. Minimum infection rates and vector indices were higher during the epidemic than in previous inter-epidemic periods, and mosquito densities exceeded thresholds considered protective against outbreaks. Entomo-virological surveillance detected a greater variety and evenness of serotypes than passive human surveillance. These findings suggest that entomo-virological surveillance can complement passive case surveillance, providing a more comprehensive detection of DENV circulation. Integrating mosquito-based and human surveillance can improve outbreak detection, guide vector control, and aid in reducing dengue burden in affected communities. Full article

The emergence of high-threat pathogens—such as Ebola, Lassa fever, and most recently SARS-CoV-2—has highlighted critical weaknesses in global surveillance systems, particularly in resource-limited settings where many zoonotic spillovers originate. Despite the World Health Organization’s (WHO) prioritization of these diseases for research and development (R&D), the current surveillance infrastructures in these regions remain under-resourced, fragmented, and often reactive rather than anticipatory. This narrative review explored the literature and structured relevant findings in three key dimensions: (i) the structural and operational limitations of existing surveillance systems for the WHO priority diseases in resource-limited settings including challenges in data integration, laboratory capacity, workforce, and community engagement; (ii) how these surveillance gaps could delay detection and hinder the response to future emerging threats, particularly a hypothetical but inevitable Disease X; and (iii) innovative and context-adapted strategies to strengthen epidemic intelligence including integrated One Health surveillance, digital and genomic tools, participatory approaches, and regional data-sharing mechanisms. We argue that building agile, equity-centered, and decentralized surveillance systems is not only essential for managing known threats, but also foundational to the early detection and rapid containment of the next public health emergency in resource-limited settings. This review uniquely frames surveillance limitations in resource-limited settings as a global security concern and outlines context-adapted, equity-centered innovations to strengthen epidemic intelligence in preparation for Disease X. Full article

Acute rhinosinusitis in children is typically caused by viral or bacterial infections. However, methicillin-resistant Staphylococcus aureus (MRSA) is increasingly linked to recurrence and severe complications, including orbital involvement. We present a case of a 6-year-old boy with periorbital swelling, proptosis, and fever following upper respiratory symptoms. Imaging revealed pansinusitis with a subperiosteal orbital abscess. He was treated empirically with intravenous ceftriaxone and metronidazole, followed by endoscopic sinus surgery. Intraoperative cultures confirmed MRSA, leading to a switch to vancomycin. The patient recovered fully without complications. This case illustrates the clinical challenges posed by MRSA sinusitis and emphasises its public health implications. Integrating antimicrobial stewardship, promoting community hygiene, ensuring early diagnostics and healthcare access, and enhancing epidemiological surveillance can play a pivotal role in reducing the burden of MRSA-related complications in children. Full article

African swine fever (ASF) is a highly contagious and fatal disease of domestic pigs and wild boars, with severe economic and ecological consequences. Wild boar hunters play a critical role in the early detection and control of ASF due to their direct interaction with wild boar populations. This study examined the attitudes, knowledge, and behaviour of wild boar hunters in Croatia regarding ASF prevention and control, with a focus on the influence of sociodemographic factors, hunting experience, and participation in training programmes. An online survey of 276 wild boar hunters from an ASF-affected county in Croatia was conducted between October and December 2024. Results indicate that 93.5% of wild boar hunters are aware of ASF and its risks, relying primarily on internet sources for information. Experienced and higher-educated wild boar hunters demonstrated greater confidence in recognising ASF symptoms and stronger support for preventive measures, education, and institutional cooperation. Factor analysis revealed three main dimensions shaping attitudes: communication and awareness, institutional capacity, and regulatory policies. The results emphasise the importance of continuous education, transparent communication and participatory approaches to strengthen cooperation with hunters and improve ASF control. They also emphasise the need for targeted, evidence-based communication strategies that actively involve hunters in surveillance and reporting. Tailored educational materials and digital communication could increase carcass reporting, improve early detection and increase the overall effectiveness of ASF control programmes. Full article

Arboviruses are a growing concern in many nations. Several reports of arboviral outbreaks have been recorded globally in the past decade alone. Repeated arboviral outbreaks in developing countries have consistently highlighted vulnerabilities in disease surveillance and response systems, exposing critical gaps in early detection, contact tracing, and resource allocation. The 2024 Dengue fever outbreak in Ghana, which recorded 205 confirmed cases out of 1410 suspected cases, underscored the urgent need to evaluate the country’s preparedness for arboviral outbreaks, given the detection of competent vectors in the country. A retrospective analysis of Ghana’s 2009–2013 pandemic influenza response plan revealed significant deficiencies in emergency preparedness, raising concerns about the country’s ability to manage emerging arboviral threats. This review assessed Ghana’s current arboviral outbreak response and preparedness by examining (a) the effectiveness of vector control measures, (b) the role of early warning systems in mitigating outbreaks, (c) laboratory support and diagnostic capabilities, and (d) community engagement strategies. It highlights the successes made in previous outbreaks and sheds light on several gaps in Ghana’s outbreak response efforts. This review also provides recommendations that can be implemented in many countries across Africa as they brace themselves for any arboviral outbreak. Full article

Lassa fever, a viral hemorrhagic illness that first came into the limelight as a clinical entity in 1969 when it was discovered in Northern Nigeria, is now found in other West African countries such as Sierra Leone, Liberia, Guinea, Togo, and the Benin Republic. Over the years, the disease, which is primarily transmitted from contact with infected mastomys rodents to humans, has the capability of secondary human-to-human transmission with significant morbidity and mortality, especially in healthcare settings. The disease is typically characterized by seasonal outbreaks, which peak during the dry season months of December to March. Lassa fever significantly impacts public health and the socioeconomic life of people in affected communities. In Nigeria, the Integrated Disease Surveillance and Response Strategy (IDSR), along with other medical countermeasures, have been employed to curtail the impact of the disease in endemic regions of Nigeria and other West Africa countries. The one-health approach to combat the disease is a promising strategy. This, along with the hope of a safe and effective vaccine, is a ray of hope on the horizon for public health authorities in Nigeria and other West African countries that the battle against Lassa fever might indeed end sooner than later. Full article

The recent spread of foreign animal diseases (FADs) such as foot-and-mouth disease and African swine fever emphasizes the need to conduct comprehensive surveillance to detect a potential disease introduction as soon as possible. The United States is currently free of many important FADs of swine, and many preparedness initiatives have raised awareness amongst the commercial, intensive swine industry. However, the awareness and engagement of small-scale swine farmers regarding disease surveillance and passive reporting is not well known. This scoping review was conducted to identify and characterize sources of evidence on the practices and attitudes of small-scale swine farmers and owners in the United States regarding pig health and disease management, surveillance, and veterinary care use, and secondarily to characterize information seeking and communication behaviors. Sources of evidence were found through keyword searches of online databases, citation matching, and Sustainable Agriculture Research and Education project reports. Eligibility criteria included being conducted on or with US small-scale (defined in this review as less than 1000 pigs) or non-intensive swine farms and containing information pertinent to the objectives of the review. Seventeen sources were included in the final review. Regular disease monitoring and surveillance practices were not commonly reported, and multiple sources reported little to no incidence of disease occurrence in small-scale swine farms. Reported veterinary use and access was variable, and multiple sources reported that the choice to use veterinary care was affected by its perceived cost, value, and accessibility. Future research and outreach should aim to discern key factors affecting farmer’s decisions to use a veterinarian, improve their awareness and prioritization of swine diseases, and develop small-scale appropriate disease surveillance protocols. Ultimately, this will help small-scale swine farmers to protect the health of their pigs and improve FAD surveillance in the US. Full article