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26 pages, 423 KiB  
Article
Pro-Environmental Behavior and Attitudes Towards Recycling in Slovak Republic
by Silvia Lorincová and Mária Osvaldová
Recycling 2025, 10(4), 159; https://doi.org/10.3390/recycling10040159 (registering DOI) - 7 Aug 2025
Abstract
Climate changes have increased interest in the circular economy, an alternative model that seeks to minimize environmental impact and maximize resource reuse. A key element of this model is individuals’ behaviors and attitudes, which determine the overall efficiency of recycling processes. The study [...] Read more.
Climate changes have increased interest in the circular economy, an alternative model that seeks to minimize environmental impact and maximize resource reuse. A key element of this model is individuals’ behaviors and attitudes, which determine the overall efficiency of recycling processes. The study fills the gap by investigating how selected socio-demographic factors affect attitudes and intentions toward recycling and material reuse in the Slovak Republic, by using the Perceived Characteristics of Innovating (PCI) framework. Through a two-way ANOVA, we tested the hypotheses that higher education correlates with stronger recycling attitudes and that women are more willing than men to engage in circular practices. The results show that gender differences in consumer attitudes towards the circular economy do occur, but their magnitude is often conditioned by education level. Education proved to be the strongest predictor of ecological behavior: respondents with higher education reported stronger beliefs in the importance of recycling and a greater willingness to act sustainably. The interaction between gender and education revealed that university-educated women hold the most pronounced pro-environmental attitudes, underscoring the importance of gender-sensitive educational strategies. It is recommended that environmental education and outreach focus on less-educated groups, particularly women, who have high potential to influence their communities. Full article
20 pages, 3001 KiB  
Article
Agroecosystem Modeling and Sustainable Optimization: An Empirical Study Based on XGBoost and EEBS Model
by Meiqing Xu, Zilong Yao, Yuxin Lu and Chunru Xiong
Sustainability 2025, 17(15), 7170; https://doi.org/10.3390/su17157170 (registering DOI) - 7 Aug 2025
Abstract
As agricultural land continues to expand, the conversion of forests to farmland has intensified, significantly altering the structure and function of agroecosystems. However, the dynamic ecological responses and their interactions with economic outcomes remain insufficiently modeled. This study proposes an integrated framework that [...] Read more.
As agricultural land continues to expand, the conversion of forests to farmland has intensified, significantly altering the structure and function of agroecosystems. However, the dynamic ecological responses and their interactions with economic outcomes remain insufficiently modeled. This study proposes an integrated framework that combines a dynamic food web model with the Eco-Economic Benefit and Sustainability (EEBS) model, utilizing empirical data from Brazil and Ghana. A system of ordinary differential equations solved using the fourth-order Runge–Kutta method was employed to simulate species interactions and energy flows under various land management strategies. Reintroducing key species (e.g., the seven-spot ladybird and ragweed) improved ecosystem stability to over 90%, with soil fertility recovery reaching 95%. In herbicide-free scenarios, introducing natural predators such as bats and birds mitigated disturbances and promoted ecological balance. Using XGBoost (Extreme Gradient Boosting) to analyze 200-day community dynamics, pest control, resource allocation, and chemical disturbance were identified as dominant drivers. EEBS-based multi-scenario optimization revealed that organic farming achieves the highest alignment between ecological restoration and economic benefits. The model demonstrated strong predictive power (R2 = 0.9619, RMSE = 0.0330), offering a quantitative basis for green agricultural transitions and sustainable agroecosystem management. Full article
(This article belongs to the Section Sustainable Agriculture)
47 pages, 11661 KiB  
Article
Reintegrating Marginalized Rural Heritage: The Adaptive Potential of Barn Districts in Central Europe’s Cultural Landscapes
by Elżbieta Komarzyńska-Świeściak and Anna Alicja Wancel
Sustainability 2025, 17(15), 7166; https://doi.org/10.3390/su17157166 (registering DOI) - 7 Aug 2025
Abstract
Barn districts—ensembles of agricultural buildings situated at the edges of rural settlements—once played a key role in the spatial and economic organization of agrarian communities in Central Europe. Today, many of these structures remain marginalized and underexplored in contemporary landscape and heritage planning. [...] Read more.
Barn districts—ensembles of agricultural buildings situated at the edges of rural settlements—once played a key role in the spatial and economic organization of agrarian communities in Central Europe. Today, many of these structures remain marginalized and underexplored in contemporary landscape and heritage planning. This paper presents a comparative study of six barn districts in Poland’s Kraków-Częstochowa Upland, where vernacular construction, ecological adaptation, and local tradition shaped distinctive rural–urban interfaces. We applied a mixed-methods approach combining cartographic and archival analysis, field surveys, and interviews with residents and experts. The research reveals consistent patterns of landscape transformation, functional decline, and latent adaptive potential across varied morphological and material typologies. Despite differing levels of preservation, barn districts retain symbolic, spatial, and socio-cultural value for communities and local landscapes. The study emphasizes the importance of reintegrating these marginal heritage structures through adaptive reuse strategies rooted in the values of the New European Bauhaus—sustainability, aesthetics, and inclusion. The findings contribute to broader discussions on rural socio-ecological resilience and landscape-based development, highlighting how place-based strategies can bridge past identities with future-oriented spatial planning. Full article
20 pages, 3576 KiB  
Article
Urban Wetland Sediments in Yangzhou: Physicochemical Properties, Microbial Communities, and Functional Associations
by Dongmei He, Liwen Li, Runyang Zhou, Sumei Qiu, Wei Xing and Yingdan Yuan
Microorganisms 2025, 13(8), 1843; https://doi.org/10.3390/microorganisms13081843 - 7 Aug 2025
Abstract
Urban wetlands play a crucial role in maintaining ecological balance, carbon sequestration, and water purification. Sediments are key carriers for wetlands to store elements such as carbon, nitrogen, and phosphorus in the aquatic environment. This study analyzed different sediment layers of seven wetlands [...] Read more.
Urban wetlands play a crucial role in maintaining ecological balance, carbon sequestration, and water purification. Sediments are key carriers for wetlands to store elements such as carbon, nitrogen, and phosphorus in the aquatic environment. This study analyzed different sediment layers of seven wetlands in Yangzhou, aiming to explore the relationship between physicochemical factors and microbial communities in wetland sediments, as well as to predict the functions of microbial communities. Functional prediction of microbial communities was conducted based on amplicon sequencing analysis, and the neutral community model was used to determine the formation and evolution process of microbial communities. The results showed that in three wetlands, namely Zhuyu Bay (ZYW), Luyang Lake (LYH), and Runyang Wetland (RYSD), the contents of carbon components (total carbon, total soluble carbon, microbial biomass carbon) in the 0–20 cm sediment layer were higher, while the carbon component contents in Baoying Lake (BYH) showed the opposite trend. Among them, the contents of total nitrogen, alkali-hydrolyzable nitrogen, total phosphorus, available phosphorus, total potassium, and available potassium in the 0–20 cm sediment layer of Runyang Wetland (RYSD) were significantly the highest. This indicates that in Runyang Wetland (RYSD), the 0–20 cm layer has more abundant carbon components and mineral nutrients compared to the 20–40 cm layer. Among the seven wetlands, it was found that the content of total potassium was all greater than 10 g/kg, which was much higher than the contents of total phosphorus and total nitrogen. Analysis of microbial communities revealed that the dominant archaeal phyla were Thaumarchaeota and Euryarchaeota, and the dominant bacterial phyla were Proteobacteria and Acidobacteria. The distribution of functional genes was mainly concentrated in Zhuyu Bay (ZYW) and Luyang Lake (LYH). Zhuyu Bay Wetland (ZYW) had potential advantages in light utilization function, and Luyang Lake (LYH) had potential advantages in carbon and nitrogen cycle functions. The assembly process of the archaeal community was mainly affected by stochastic processes, while the bacterial community was mainly affected by deterministic processes. However, water content, total phosphorus, and available potassium all had strong correlations with both archaeal and bacterial communities. The research results preliminarily reveal the connections between the physicochemical properties of sediments, microbial communities, and their potential functions in Yangzhou urban wetlands, providing an important scientific basis for the protection and management of wetland ecosystems. Full article
(This article belongs to the Section Environmental Microbiology)
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19 pages, 4925 KiB  
Article
Environmental Heterogeneity Drives Diversity Across Forest Strata in Hopea hainanensis Communities
by Shaocui He, Donghai Li, Xiaobo Yang, Dongling Qi, Naiyan Shang, Caiqun Liang, Rentong Liu and Chunyan Du
Diversity 2025, 17(8), 556; https://doi.org/10.3390/d17080556 - 7 Aug 2025
Abstract
Species and phylogenetic diversity play vital roles in sustaining the structure, function, and resilience of plant communities, particularly in tropical rainforests. However, the mechanisms according to which environmental filtering and competitive exclusion influence diversity across forest layers remain insufficiently understood. In this study, [...] Read more.
Species and phylogenetic diversity play vital roles in sustaining the structure, function, and resilience of plant communities, particularly in tropical rainforests. However, the mechanisms according to which environmental filtering and competitive exclusion influence diversity across forest layers remain insufficiently understood. In this study, we investigated the species and phylogenetic diversity patterns in two representative tropical rainforest sites—Bawangling and Jianfengling—within Hainan Tropical Rainforest National Park, China, focusing on communities associated with the endangered species Hopea hainanensis. We employed a one-way ANOVA and Pearson’s correlation analyses to examine the distribution characteristics and interrelationships among diversity indices and used Mantel tests to assess the correlations with environmental variables. Our results revealed that the plant community in Jianfengling exhibited a significantly higher species richness at the family, genus, and species levels (a total of 288 plant species have been recorded, belonging to 82 families and 183 genera) compared to that in Bawangling (a total of 212 plant species, belonging to 75 families and 162 genera). H. hainanensis held the highest importance value in the middle tree layer across both sites (IV(BWL) = 12.44; IV(JFL) = 5.73), while dominant species varied notably among other forest layers, indicating strong habitat specificity. Diversity indices, including the Simpson index, the Shannon–Wiener index, and Pielou’s evenness, were significantly higher in the large shrub layer of Jianfengling, whereas Bawangling showed a relatively higher Shannon–Wiener index in the middle shrub layer. Phylogenetic diversity (PD) and the phylogenetic structure indices (NRI and NTI) displayed distinct vertical stratification patterns between sites. Furthermore, the PD in Bawangling’s large shrub layer was positively correlated with total phosphorus in the soil, while community evenness was influenced by soil organic carbon and total nitrogen. In Jianfengling, species richness was significantly associated with soil bulk density, altitude, and pH. These findings enhance our understanding of the ecological and evolutionary processes shaping biodiversity in tropical rainforests and highlight the importance of incorporating both species and phylogenetic metrics into the conservation strategies for endangered species such as Hopea hainanensis. Full article
(This article belongs to the Special Issue Biodiversity Conservation Planning and Assessment—2nd Edition)
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14 pages, 2183 KiB  
Article
Interannual Variations in Soil Bacterial Community Diversity and Analysis of Influencing Factors During the Restoration Process of Scirpus Mariqueter Wetlands
by Yaru Li, Shubo Fang, Qinyi Wang, Pengling Wu, Peimin He and Wei Liu
Biology 2025, 14(8), 1013; https://doi.org/10.3390/biology14081013 - 7 Aug 2025
Abstract
Due to human activities and the invasion of Spartina alterniflora, the population of Scirpus mariqueter (S. mariqueter) in the Yangtze River Estuary has gradually declined. To address this issue, numerous restoration efforts have been undertaken. To investigate the changes and [...] Read more.
Due to human activities and the invasion of Spartina alterniflora, the population of Scirpus mariqueter (S. mariqueter) in the Yangtze River Estuary has gradually declined. To address this issue, numerous restoration efforts have been undertaken. To investigate the changes and influencing factors of soil bacterial communities during the restoration of S. mariqueter wetlands, we selected S. mariqueter populations as the research focus and divided the samples into two years, S1 and S2. High-throughput sequencing technology was employed for observation and analysis. The results revealed that from S1 to S2, soil bacterial diversity in the S. mariqueter wetland increased significantly and displayed clear seasonal patterns. The dominant bacterial phyla included Proteobacteria, Bacteroidota, Firmicutes, and Acidobacteriota. Among these, Proteobacteria had the highest relative abundance, while Acidobacteriota showed the most pronounced increase, surpassing Bacteroidota and Firmicutes to become the second most abundant group. Redundancy analysis (RDA) indicated that soil organic matter and electrical conductivity were the key factors influencing the composition and diversity of the soil bacterial community, with Acidobacteriota playing a dominant role during wetland restoration. In conclusion, during the ecological restoration of the S. mariqueter wetlands, attention should be given to environmental factors such as soil organic matter and electrical conductivity, while the regulatory role of Acidobacteriota in wetland soils should not be overlooked. This study provides a microscopic perspective on the interactions between microbial diversity and ecosystem functions in coastal wetlands, offering valuable guidance for the ecological restoration of degraded wetlands. Full article
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20 pages, 1014 KiB  
Review
State of the Art on the Interaction of Entomopathogenic Nematodes and Plant Growth-Promoting Rhizobacteria to Innovate a Sustainable Plant Health Product
by Islam Ahmed Abdelalim Darwish, Daniel P. Martins, David Ryan and Thomais Kakouli-Duarte
Crops 2025, 5(4), 52; https://doi.org/10.3390/crops5040052 - 6 Aug 2025
Abstract
Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground [...] Read more.
Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground waters, and is hazardous to soil biota. Therefore, applications of entomopathogenic nematodes (EPNs) and plant growth-promoting rhizobacteria (PGPR) are an alternative, eco-friendly solution to chemical pesticides and mineral-based fertilizers to enhance plant health and promote sustainable food security. This review focuses on the biological and ecological aspects of these organisms while also highlighting the practical application of molecular communication approaches in developing a novel plant health product. This insight will support this innovative approach that combines PGPR and EPNs for sustainable crop production. Several studies have reported positive interactions between nematodes and bacteria. Although the combined presence of both organisms has been shown to promote plant growth, the molecular interactions between them are still under investigation. Integrating molecular communication studies in the development of a new product could help in understanding their relationships and, in turn, support the combination of these organisms into a single plant health product. Full article
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14 pages, 7789 KiB  
Article
Integrated Sampling Approaches Enhance Assessment of Saproxylic Beetle Biodiversity in a Mediterranean Forest Ecosystem (Sila National Park, Italy)
by Federica Mendicino, Francesco Carlomagno, Domenico Bonelli, Erica Di Biase, Federica Fumo and Teresa Bonacci
Insects 2025, 16(8), 812; https://doi.org/10.3390/insects16080812 - 6 Aug 2025
Abstract
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase [...] Read more.
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase the detection of species with varying ecological traits. We evaluated the effectiveness of integrative sampling methodologies to assess saproxylic beetle diversity within Sila National Park, a Mediterranean forest ecosystem of high conservation value, specifically in two beech forests and four pine forests. The sampling methods tested included Pan Traps (PaTs), Malaise Traps (MTs), Pitfall Traps (PTs), Bait Bottle Traps (BBTs), and Visual Census (VC). All specimens were identified to the species level whenever possible, using specialized dichotomous keys and preserved in the Entomological Collection TB, Unical. Various trap types captured a different number of species: the PaT collected 32 species, followed by the PT with 24, the MT with 16, the VC with 7, and the BBT with 5 species. Interestingly, biodiversity analyses conducted using PAST software version 4.17 revealed that PaTs and MTs recorded the highest biodiversity indices. The GLMM analysis, performed using SPSS software 29.0.1.0, demonstrated that various traps attracted different species with different abundances. By combining multiple trapping techniques, we documented a more comprehensive community composition compared to single-method approaches. Moreover, PaTs, MTs, and PTs recorded 20%, 40%, and 33% of the Near Threatened species, respectively. We report new records for Sila National Park, including the LC species Pteryngium crenulatum (Curculionidae) and the NT species Grynocharis oblonga (Trogossitidae). For the first time in Calabria, the LC species Triplax rufipes (Erotylidae) and the NT species Oxypleurus nodieri (Cerambycidae) and Glischrochilus quadrisignatus (Nitidulidae) were collected. Our results emphasize the importance of method diversity in capturing species with distinct ecological requirements and highlight the relevance of saproxylic beetles as indicators of forest health. These findings support the adoption of multi-method sampling protocols in forest biodiversity monitoring and management programs, especially in biodiversity-rich and structurally heterogeneous landscapes. Full article
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18 pages, 2357 KiB  
Article
Nitrogen Fertilizer Reduction in Rice–Eel Co-Culture System Improves the Soil Microbial Diversity and Its Functional Stability
by Mengqian Ma, Weiguang Lv, Yu Huang, Juanqin Zhang, Shuangxi Li, Naling Bai, Haiyun Zhang, Xianpu Zhu, Chenglong Xu and Hanlin Zhang
Plants 2025, 14(15), 2425; https://doi.org/10.3390/plants14152425 - 5 Aug 2025
Abstract
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began [...] Read more.
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began in 2016 and was sampled in 2023, the effects of reduced nitrogen fertilizer application on soil physico-chemical properties and the bacterial community were investigated. Treatments included a conventional regular fertilization treatment (RT), rice–eel co-culture system regular fertilization (IT), and nitrogen-reduction 10%, 30%, and 50% fertilization treatments (IT90, IT70, and IT50). Our research demonstrated the following: (1) Compared to RT, IT significantly increased soil water-stable macroaggregates (R0.25), mean weight diameter (MWD), geometric mean diameter (GMD), and available phosphorus content, with the increases of 15.66%, 25.49%, 36.00%, and 18.42%, respectively. Among the nitrogen-reduction fertilization treatments, IT90 showed the most significant effect. Compared to IT, IT90 significantly increased R0.25, MWD, GMD, and available nitrogen content, with increases of 4.4%, 7.81%, 8.82%, and 28.89%, respectively. (2) Compared to RT, at the phylum level, the diversity of Chloroflexi was significantly increased under IT and IT50, and the diversity of Gemmatimonadota was significantly increased under IT90, IT70, and IT50. The diversity of Acidobacteriota was significantly higher in IT90 and IT70 compared to IT. It was shown that the rice–eel co-culture system and nitrogen fertilizer reduction could effectively improve the degradation capacity of organic matter and promote soil nitrogen cycling. In addition, redundancy analysis (RDA) identified total phosphorus, total nitrogen, and available nitrogen (p = 0.007) as the three most important environmental factors driving changes in the bacterial community. (3) The functional prediction analysis of soil microbiota showed that, compared to RT, the diversity of pathways related to biosynthesis (carbohydrate biosynthesis and cell structure biosynthesis) and metabolism (L-glutamate and L-glutamine biosynthesis) was significantly higher under IT70, IT90, IT, and IT50 (in descending order). However, the diversity of pathways associated with degradation/utilization/assimilation (secondary metabolite degradation and amine and polyamine degradation) was significantly lower under all the rice–eel co-culture treatments. In conclusion, the rice–eel co-culture system improved soil physicochemical properties and the soil microbial environment compared with conventional planting, and the best soil improvement was achieved with 10% less N fertilizer application. Full article
(This article belongs to the Special Issue Chemical Properties of Soils and its Impact on Plant Growth)
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19 pages, 1022 KiB  
Review
Leishmania in Texas: A Contemporary One Health Scoping Review of Vectors, Reservoirs, and Human Health
by Morgan H. Jibowu, Richard Chung, Nina L. Tang, Sarah Guo, Leigh-Anne Lawton, Brendan J. Sullivan, Dawn M. Wetzel and Sarah M. Gunter
Biology 2025, 14(8), 999; https://doi.org/10.3390/biology14080999 - 5 Aug 2025
Viewed by 1
Abstract
Leishmaniasis, a vector-borne neglected tropical disease, affects over 6.2 million people globally. Case acquisition is increasingly recognized in the United States, and in Texas, most reported cases are locally acquired and speciated to Leishmania mexicana. We conducted a scoping literature review to [...] Read more.
Leishmaniasis, a vector-borne neglected tropical disease, affects over 6.2 million people globally. Case acquisition is increasingly recognized in the United States, and in Texas, most reported cases are locally acquired and speciated to Leishmania mexicana. We conducted a scoping literature review to systematically assess contemporary research on Leishmania in humans, animals, reservoir hosts, or vectors in Texas after 2000. Out of 22 eligible studies, the most prevalent themes were case reports, followed by studies on domestic animals, reservoirs, and vectors, with several studies bridging multiple disciplines. Climate change, urbanization, and habitat encroachment appear to be driving the northward expansion of L. mexicana, which is primarily attributed to shifts in the habitats of key vectors (Lutzomyia anthophora) and reservoirs (Neotoma spp.). Leishmania appears to be expanding into new areas, with potential for further spread. As ecological conditions evolve, strengthening surveillance and clinician awareness is crucial to understanding disease risk and improving early detection and treatment in affected communities. Full article
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24 pages, 34309 KiB  
Article
Assessing the Motile Fauna of Eastern Mediterranean Marine Caves
by Markos Digenis, Michail Ragkousis, Charalampos Dimitriadis, Stelios Katsanevakis and Vasilis Gerovasileiou
Fishes 2025, 10(8), 383; https://doi.org/10.3390/fishes10080383 - 5 Aug 2025
Viewed by 32
Abstract
Although marine caves are among the most species-diverse habitats in the Mediterranean Sea, most available studies have focused on their sessile fauna. This study provides the first quantitative assessment of motile fauna in 27 marine caves across four geographical subareas of the Aegean [...] Read more.
Although marine caves are among the most species-diverse habitats in the Mediterranean Sea, most available studies have focused on their sessile fauna. This study provides the first quantitative assessment of motile fauna in 27 marine caves across four geographical subareas of the Aegean and Ionian Seas, using a rapid assessment visual census protocol, applied through 3 min time transects in each ecological cave zone. Multivariate analysis revealed that the motile community structure of the cave entrance was differentiated from that of the semidark and dark zones. Deeper caves were distinct from shallower ones while caves of the east Aegean differed from those around Crete Island. A total of 163 taxa were recorded, 27 of which are reported herein for the first time in marine caves of the eastern Mediterranean Sea, while three species (two native and one introduced) are recorded in Greek waters for the first time, enriching our knowledge on the permanent and occasional cave residents. Seventeen species were introduced, comprising more than half of the total fish abundance in the southeasternmost cave. Our limited knowledge of the motile fauna of Mediterranean marine caves coupled with the continued spread of introduced species highlights the urgent need for monitoring and conservation actions, especially within marine protected areas. Full article
(This article belongs to the Section Biology and Ecology)
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10 pages, 481 KiB  
Review
Bacterial–Fungal Interactions: Mutualism, Antagonism, and Competition
by Manyu Zhang, Yuwei Zhang, Zhengge Zhao, Feilong Deng, Hui Jiang, Ce Liu, Ying Li and Jianmin Chai
Life 2025, 15(8), 1242; https://doi.org/10.3390/life15081242 - 5 Aug 2025
Viewed by 51
Abstract
The interaction between bacteria and fungi is one of the key interactions of microbial ecology, including mutualism, antagonism, and competition, which profoundly affects the balance and functions of animal microbial ecosystems. This article reviews the interactive dynamics of bacteria and fungi in more [...] Read more.
The interaction between bacteria and fungi is one of the key interactions of microbial ecology, including mutualism, antagonism, and competition, which profoundly affects the balance and functions of animal microbial ecosystems. This article reviews the interactive dynamics of bacteria and fungi in more concerned microenvironments in animals, such as gut, rumen, and skin. Moreover, we summarize the molecular mechanisms and ecological functions of the interaction between bacteria and fungi. Three major bacterial–fungal interactions (mutualism, antagonism, and competition) are deeply discussed. Understanding of the interactions between bacteria and fungi allows us to understand, modulate, and maintain the community structure and functions. Furthermore, this summarization will provide a comprehensive perspective on animal production and veterinary medicine, as well as guide future research directions. Full article
(This article belongs to the Special Issue Gut Microbes Associating with the Host)
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17 pages, 12216 KiB  
Article
Green/Blue Initiatives as a Proposed Intermediate Step to Achieve Nature-Based Solutions for Wildfire Risk Management
by Stella Schroeder and Carolina Ojeda Leal
Fire 2025, 8(8), 307; https://doi.org/10.3390/fire8080307 - 5 Aug 2025
Viewed by 154
Abstract
Implementing nature-based solutions (NbSs) for wildfire risk management and other hazards has been challenging in emerging economies due to the high costs, the lack of immediate returns on investment, and stringent inclusion criteria set by organizations like the IUCN and domain experts. To [...] Read more.
Implementing nature-based solutions (NbSs) for wildfire risk management and other hazards has been challenging in emerging economies due to the high costs, the lack of immediate returns on investment, and stringent inclusion criteria set by organizations like the IUCN and domain experts. To address these challenges, this exploratory study proposes a new concept: green/blue initiatives. These initiatives represent intermediate steps, encompassing small-scale, community-driven activities that can evolve into recognized NbSs over time. To explore this concept, experiences related to wildfire prevention in the Biobío region of Chile were analyzed through primary and secondary source reviews. The analysis identified three initiatives qualifying as green/blue initiatives: (1) goat grazing in Santa Juana to reduce fuel loads, (2) a restoration prevention farm model in Florida called Faro de Restauración Mahuidanche and (3) the Conservation Landscape Strategy in Nonguén. They were examined in detail using data collected from site visits and interviews. In contrast to Chile’s prevailing wildfire policies, which focus on costly, large-scale fire suppression efforts, these initiatives emphasize the importance of reframing wildfire as a manageable ecological process. Lastly, the challenges and enabling factors for adopting green/blue initiatives are discussed, highlighting their potential to pave the way for future NbS implementation in central Chile. Full article
(This article belongs to the Special Issue Nature-Based Solutions to Extreme Wildfires)
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28 pages, 974 KiB  
Review
Murburn Bioenergetics and “Origins–Sustenance–Termination–Evolution of Life”: Emergence of Intelligence from a Network of Molecules, Unbound Ions, Radicals and Radiations
by Laurent Jaeken and Kelath Murali Manoj
Int. J. Mol. Sci. 2025, 26(15), 7542; https://doi.org/10.3390/ijms26157542 - 5 Aug 2025
Viewed by 255
Abstract
The paradigm-shift idea of murburn concept is no hypothesis but developed directly from fundamental facts of cellular/ecological existence. Murburn involves spontaneous and stochastic interactions (mediated by murzymes) amongst the molecules and unbound ions of cells. It leads to effective charge s [...] Read more.
The paradigm-shift idea of murburn concept is no hypothesis but developed directly from fundamental facts of cellular/ecological existence. Murburn involves spontaneous and stochastic interactions (mediated by murzymes) amongst the molecules and unbound ions of cells. It leads to effective charge separation (ECS) and formation/recruitment of diffusible reactive species (DRS, like radicals whose reactions enable ATP-synthesis and thermogenesis) and emission of radiations (UV/Vis to ELF). These processes also lead to a chemo-electromagnetic matrix (CEM), ascertaining that living cell/organism react/function as a coherent unit. Murburn concept propounds the true utility of oxygen: generating DRS (with catalytic and electrical properties) on the way to becoming water, the life solvent, and ultimately also leading to phase-based macroscopic homeostatic outcomes. Such a layout enables cells to become simple chemical engines (SCEs) with powering, coherence, homeostasis, electro-mechanical and sensing–response (PCHEMS; life’s short-term “intelligence”) abilities. In the current review, we discuss the coacervate nature of cells and dwell upon the ways and contexts in which various radiations (either incident or endogenously generated) could interact in the new scheme of cellular function. Presenting comparative evidence/arguments and listing of systems with murburn models, we argue that the new perceptions explain life processes better and urge the community to urgently adopt murburn bioenergetics and adapt to its views. Further, we touch upon some distinct scientific and sociological contexts with respect to the outreach of murburn concept. It is envisaged that greater awareness of murburn could enhance the longevity and quality of life and afford better approaches to therapies. Full article
(This article belongs to the Section Molecular Biophysics)
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26 pages, 884 KiB  
Review
Harnessing Seed Endophytic Microbiomes: A Hidden Treasure for Enhancing Sustainable Agriculture
by Ayomide Emmanuel Fadiji, Adedayo Ayodeji Lanrewaju, Iyabo Olunike Omomowo, Fannie Isela Parra-Cota and Sergio de los Santos-Villalobos
Plants 2025, 14(15), 2421; https://doi.org/10.3390/plants14152421 - 4 Aug 2025
Viewed by 201
Abstract
Microbes perform diverse and vital functions in animals, plants, and humans, and among them, plant-associated microbiomes, especially endophytes, have attracted growing scientific interest in recent years. Numerous plant species thriving in diverse environments have been shown to host endophytic microbes. While endophytic bacteria [...] Read more.
Microbes perform diverse and vital functions in animals, plants, and humans, and among them, plant-associated microbiomes, especially endophytes, have attracted growing scientific interest in recent years. Numerous plant species thriving in diverse environments have been shown to host endophytic microbes. While endophytic bacteria commonly colonize plant tissues such as stems, roots, and leaves, seed-associated endophytes generally exhibit lower diversity compared to those in other plant compartments. Nevertheless, seed-borne microbes are of particular importance, as they represent the initial microbial inoculum that influences a plant’s critical early developmental stages. The seed endophytic microbiome is of particular interest due to its potential for vertical transmission and its capacity to produce a broad array of phytohormones, enzymes, antimicrobial compounds, and other secondary metabolites. Collectively, these functions contribute to enhanced plant biomass and yield, especially under abiotic and biotic stress conditions. Despite their multifaceted roles, seed microbiomes remain underexplored in plant ecology, and their potential benefits are not yet fully understood. This review highlights recent advances in our understanding of the diversity, community composition, mechanisms of action, and agricultural significance of seed endophytic microbes. Furthermore, it synthesizes current insights into how seed endophytes promote plant health and productivity and proposes future research directions to fully harness their potential in sustainable agriculture. Full article
(This article belongs to the Section Plant Protection and Biotic Interactions)
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