Search Results (970)

Fungal communities associated with the phyllosphere can influence plant health, stress responses, and disease dynamics in managed crop systems. However, limited information is available on fungal microbiome structure across phyllosphere compartments of Eucalyptus cinerea cultivated for cut foliage production. In this study, fungal communities (including epiphytic and endophytic fungi) associated with leaf, stem, and bark tissues of intensively managed E. cinerea grown in Ireland were characterised using ITS amplicon sequencing. Samples were collected from five trees, with tissues pooled by compartment to generate 15 biological samples. Following quality control and denoising, 405 fungal amplicon sequence variants (ASVs) were retained for analysis. Observed richness, Shannon and Simpson indices, and Faith’s phylogenetic diversity differed among compartments, with bark exhibiting higher values than leaf and stem tissues (p < 0.05). PERMANOVA analysis indicated that both compartment (R² = 0.239, p = 0.002) and tree identity (R² = 0.451, p = 0.002) significantly influenced fungal community composition. Bark communities were dominated by Diaporthe (52.9%), Peniophora (12.8%), and Talaromyces (10.4%), whereas leaf and stem communities were characterised primarily by Vishniacozyma and Sporobolomyces. Differential abundance analysis identified 26 and 23 differentially abundant ASVs between bark and leaf, and bark and stem tissues, respectively, whereas no significant differences were detected between leaf and stem communities. Weighted UniFrac analyses further revealed separation of bark-associated communities from photosynthetic tissues. These findings demonstrate compartment-associated variation in fungal community structure within the phyllosphere of managed E. cinerea and highlight the importance of considering both host-level and tissue-level effects in plant microbiome studies. This study provides a baseline assessment of fungal assemblages associated with commercially managed Eucalyptus under Irish growing conditions and supports future investigations into the functional significance of these microbial communities for plant health and resilience. Full article

The urgency for sustainable propagation methods is increasing as anthropogenic disturbances threaten global biodiversity and plant productivity. This study focused on the cutting propagation of Campanula pelviformis and Petromarula pinnata, two local endemic species of Crete (Greece) with known multipurpose crop potential. For C. pelviformis, the effects of cutting type and indole-3-butyric acid (IBA) treatments applied with or without the commercially available and environmentally friendly fertilizer Theocopper and population were investigated. For P. pinnata, the effects of cutting type and IBA treatments were examined. Rooting success and root dry mass were assessed three weeks after propagation onset, while root number and length were measured in the third week to evaluate overall rooting performance. Vegetative propagation of P. pinnata was straightforwardly achieved across cutting types (>90% rooting success), and superior root traits were observed in young shoot cuttings. In C. pelviformis, high rooting success (90%) was recorded in four of the six treatments using adventitious shoot cuttings, while the combined application of 1% Theocopper and IBA efficiently enhanced both rooting percentages and root traits in young shoot cuttings and sub-apical stem sections. The results further indicated a population effect on rooting success and traits among cutting types. The data produced may guide conservation purposes and/or sustainable utilization of these neglected species as novel multipurpose crops, especially in landscaping design with native plants. Full article

Effective thermal management is critical for ensuring the reliability of modern high-power electronic devices, where thermal interface materials (TIMs) play key roles in minimizing contact resistance and improving heat dissipation. Boron nitride (BN) is widely used as a thermally conductive filler due to its high in-plane thermal conductivity and electrical insulation. However, achieving BN-based polymer composites that simultaneously offer high filler loading, flexibility, and high thermal conductivity (κ) remains a significant challenge. In this work, we introduce a novel two-step fabrication strategy to overcome this limitation. First, continuous BN fibers with high aspect ratios are assembled into BN fiber papers with enhanced fiber alignment. These papers are then cut and integrated into a silicone matrix to form well-oriented thermal conductive channels. This approach enables a significantly higher filler mass fraction of 70%, resulting in a thermal pad with a high κ of 19.23 W/(m·K), low thermal resistance of 1.61 cm²·K/W, and excellent electrical insulation and flexibility. Application tests further demonstrate superior heat dissipation performance and operational stability compared to commercial silicone pads. This work not only highlights the potential of BN fiber-based TIMs but also offers a feasible process for their large-scale manufacturing. Full article

Adventitious rooting remains a major constraint for the clonal propagation of Coffea arabica, limiting the large-scale multiplication of elite genotypes. This study evaluated the effects of genotype, auxin treatment, and their interaction on adventitious rooting and basal callus formation in coffee cuttings under controlled nursery conditions, while morphophysiological traits were assessed as complementary indicators of cutting performance. A completely randomized 3 × 3 factorial design was used, including three hybrids (H3, Excelencia, and Milenio) and three plant growth regulator (PGR) treatments: control, indole-3-butyric acid (IBA), and a commercial auxin formulation (RH; NAA + IBA). Rooting probability and root number were significantly affected by PGR treatment, whereas the longest root length was influenced by hybrid, PGR treatment, and their interaction. Model-estimated rooting probability increased from 4.76% in the control to 17.05% under IBA and 35.73% under RH. Similarly, the estimated number of roots per cutting increased from 0.14 in the control to 0.99 under IBA and 1.85 under RH. Although the hybrid × PGR interaction was not significant for rooting probability, the highest observed rooting percentage was recorded in H3 under RH (52.38%), followed by Milenio under RH (33.33%). For the longest root length, the strongest responses were observed under RH, particularly in Milenio (71.15 mm) and H3 (70.08 mm). Callus formation varied among treatments, but its association with rooting performance was weak and inconsistent. Morphophysiological traits provided complementary information on cutting status but were not interpreted as direct mechanistic drivers of rooting. These findings indicate that adventitious rooting in C. arabica was more closely associated with genotype-dependent responsiveness to exogenous auxin than with the extent of callus formation. However, anatomical studies are needed to determine the developmental origin of root primordia and their possible relationship with callus tissue. Full article

Gabiroba (Campomanesia adamantium (Cambess.) O. Berg) is a native fruit tree of the Brazilian Cerrado biome with notable economic potential and phytotherapeutic properties. However, commercial seedling production is limited by the recalcitrant nature of its seeds and vegetative propagation is further constrained by phenolic compound oxidation. Therefore, this study evaluated the effects of antioxidant treatments on sprouting, cutting survival, callus formation and attempted adventitious rooting of C. adamantium cuttings collected in different seasons. The experiment was conducted in a greenhouse at the Federal University of Jataí using cuttings collected from an experimental orchard. Eleven treatments were evaluated: a control and different concentrations of ascorbic acid (AA; 10, 20, and 40 mg L⁻¹), phloroglucinol (FLO; 50, 100, and 200 mg L⁻¹), and polyvinylpyrrolidone (PVP; 100, 200, 400, and 600 mg L⁻¹). After 60 days, sprouting, cutting survival, callus formation, rooting, and the number of old and new leaves were assessed. Data were subjected to analysis of variance, and means were compared using the Scott–Knott test. Antioxidant treatments did not promote rhizogenesis in C. adamantium cuttings. Nevertheless, the season influenced sprouting, survival, and callus formation, especially during summer and autumn. These results indicate that antioxidant application alone is insufficient to overcome rooting recalcitrance in this species, although it may affect early developmental responses of the cuttings under specific seasonal conditions. Full article

Commercial V₂O₅ powders typically exhibit a lamellar morphology with limited structural stability and sluggish electron/ion transport, which restricts their discharge performance in thermal batteries. This work aims to enhance the discharge performance of V₂O₅ cathodes by constructing a robust spherical architecture via a scalable spray drying strategy combined with carbon modification. The as-prepared V₂O₅/C cathode delivers a high initial discharge voltage of 2.45 V, a specific capacity of 261.06 mAh g⁻¹, and an energy density of 591.05 Wh kg⁻¹ at 0.1 A cm⁻² and 500 °C (cut-off voltage of 1.9 V), outperforming those of commercial V₂O₅ cathodes. Pulse discharge tests and resistance evolution analyses further demonstrate enhanced voltage stability and reduced interfacial resistance. These improvements originate from the synergistic effect of the spherical architecture and conductive carbon network, which facilitates continuous electron/ion transport and reinforces structural integrity under high-temperature discharge conditions. This work provides a scalable design strategy for high-tap-density spherical V₂O₅ cathodes and offers insight into the coupling among morphology, conductivity, and stability in cathode materials for thermal battery. Full article

Fruit harvesting systems are undergoing a paradigm shift toward sustainable and energy-efficient mechanized platforms driven by robotics, artificial intelligence, and advanced sensing technologies. This review synthesizes recent engineering developments in fruit harvesting, focusing on system architecture, fruit detachment mechanics, and mechanized harvesting strategies. It examines harvesting classifications, mechanical principles governing detachment, and pre-harvest factors affecting performance, along with principal mechanisms including shaking, cutting, and alternative detachment techniques. Post-detachment handling and fruit recovery processes are also analyzed, together with economic and sustainability-related trade-offs between manual and mechanized harvesting systems. Recent progress in robotic harvesting systems, machine vision, and multi-sensor fusion is evaluated within the framework of smart orchard engineering, with increasing emphasis on energy-efficient design, resource optimization, reduced postharvest losses, and environmental sustainability as key performance drivers. Despite these advancements, current technologies remain constrained by fruit damage susceptibility, biological variability, limited cross-crop adaptability, and high implementation costs, limiting large-scale adoption in commercial orchards. The novelty of this review lies in establishing a unified engineering framework that links mechanical detachment principles with robotic systems and intelligent sensing technologies under an energy-efficient sustainability perspective, enabling a system-level understanding of harvesting performance and supporting the development of next-generation adaptive and sustainable fruit harvesting systems. Full article

Aqueous zinc–iodine batteries (AZIBs) are emerging as highly promising candidates for next-generation, grid-scale energy storage due to the intrinsic safety of water-based electrolytes, the high theoretical capacity of the zinc anode, and the rapid conversion kinetics of the iodine cathode. However, the practical commercialization of AZIBs is severely impeded by formidable interfacial instabilities, including the uncontrollable growth of zinc dendrites, parasitic hydrogen evolution reactions (HER), and the notorious polyiodide (I₃⁻, I₅⁻) shuttle effect. These macroscopic degradation modes are fundamentally rooted in the robust [Zn(H₂O)₆]²⁺ primary solvation sheath and the immense thermodynamic driving force for polyiodide dissolution in highly polar aqueous media. To address these interconnected challenges, electrolyte engineering has evolved into the most potent, holistic strategy. This comprehensive review systematically evaluates the latest advancements in electrolyte engineering for AZIBs. We first deeply decipher the fundamental thermodynamic mechanisms governing Zn²⁺ desolvation and iodine multiphase conversion. Subsequently, we critically analyze cutting-edge regulation paradigms, including water-in-salt (WIS) and localized high-concentration electrolytes (LHCE), cosolvent networks, functional molecular additives, deep eutectic solvents (DES), and quasi-solid-state hydrogels. By integrating in situ/operando spectroscopic characterizations with multiscale theoretical computations (such as MD and DFT), we elucidate the structure–activity relationships at the atomic level. Finally, we provide strategic perspectives on the future trajectories of the field, emphasizing the stabilization of multi-electron (I⁻/I⁰/I⁺) halogen chemistry, AI-driven high-throughput screening, and the rigorous standardization of Ah-level pouch cell engineering for extreme-environment applications. Full article

Sunflower is predominantly cultivated and extensively studied as an oil-type crop, which has contributed to the development of broad genetic variability within oil-type germplasm. In contrast, ornamental sunflower has received considerably less research attention. Therefore, the existing genetic variability of oil-type sunflower represents a valuable resource that can be explored and tested for ornamental traits, including postharvest performance and suitability for cut-flower production. This is particularly relevant given the increasing market demand for decorative sunflower types and the growing interest in diversified cut-flower assortments. The objective of this study was to assess the ornamental potential of oil-type sunflower genotypes, with particular emphasis on postharvest performance of cut-flowers. Genotypes from the breeding collection of the Institute of Field and Vegetable Crops, Novi Sad, were evaluated for key ornamental morphological traits and postharvest longevity. The experiment comprised treatments combining two storage regimes (room conditions and chamber storage) and two holding solutions (distilled water and 10% sucrose), applied across two sowing dates. To identify possible structural determinants of postharvest behavior, three morphologically contrasting genotypes (ĆMD U 12, AS 87, and LIP P 98) were further subjected to anatomical analysis of the peduncle. Postharvest longevity was significantly affected by genotype, sowing date, storage regime, sucrose treatment, and their interactions, demonstrating the strong influence of both genetic background and postharvest handling conditions. Chamber storage consistently affected postharvest longevity in most genotypes, while sucrose supplementation further improved it in several genotypes. Substantial genotypic variation in postharvest performance was detected. Substantial genotypic variation in postharvest performance was detected. Genotype ĆMD U 12 exhibited outstanding postharvest longevity under several storage environments, particularly in the second sowing date, whereas AS 87 showed markedly reduced postharvest longevity, particularly under ambient storage conditions. Morphological traits alone were not reliable predictors of postharvest longevity. In contrast, anatomical analyses revealed clear differences in peduncle tissue organization and vascular architecture among the selected genotypes, indicating that variation in supportive tissues and xylem vessel characteristics may play an important role in postharvest water relations and flower postharvest longevity. The results demonstrate the potential of IFVCNS oil-type sunflower germplasm for developing ornamental cut-flower genotypes adapted to dense sowing conditions, while also identifying postharvest treatments that significantly improve postharvest longevity and commercial usability. Full article

Cephalopod products are widely distributed as frozen raw materials or cut portions, making morphology-based species identification difficult during commercial handling and inspection. In this study, we developed a conventional multiplex PCR assay for the simultaneous identification of six commercially important cephalopod species, Octopus vulgaris, O. ocellatus, O. minor, Enteroctopus dofleini, Dosidicus gigas, and Todarodes pacificus. Species-specific forward primers and a shared reverse primer were designed from the mitochondrial cytochrome c oxidase subunit I (COI) region to generate distinct diagnostic amplicons within a single reaction. The assay successfully produced species-resolved bands of 459, 365, 248, 194, 141, and 82 bp for O. vulgaris, E. dofleini, O. ocellatus, O. minor, D. gigas, and T. pacificus, respectively, with no ambiguous overlap among diagnostic fragments. Clear and reproducible amplification was obtained at annealing temperatures of 51–54 °C, with 52 °C selected as the standard condition, indicating useful operational tolerance for routine application. The assay also retained consistent diagnostic performance down to 1 ng of template DNA per reaction. These results demonstrate that the developed multiplex PCR assay provides a simple, rapid, and gel-based method for the preliminary identification of selected cephalopod species in frozen commercial materials and may be useful for seafood inspection and market surveillance. Full article

The increasing worldwide demand for sustainable energy and effective waste management has heightened interest in solutions. Microbial fuel cells (MFCs) represent a potential category of bioelectrochemical systems that directly transform the chemical energy contained in organic waste into electrical energy via the metabolic processes of electroactive microorganisms. In the last twenty years, significant advancements have occurred in the comprehension of extracellular electron transfer (EET) mechanisms, biofilm formation, microbial community dynamics, electrode material engineering, and reactor design, resulting in marked enhancements in power density and wastewater treatment efficacy. Despite these breakthroughs, the extensive deployment and commercialization of MFC technology are constrained by various hurdles, including inadequate energy recovery, elevated material and fabrication expenses, operational instability, and the intricacies of system scale-up. This cutting-edge analysis offers a thorough evaluation of recent advancements in MFCs and their incorporation with sophisticated technology for waste management and energy generation. Focus is directed towards essential bioelectrochemical principles, microbial and biofilm engineering techniques, sophisticated electrode and membrane materials, reactor designs, and hybrid MFC systems integrated with anaerobic digestion, microbial electrolysis, and advanced oxidation methods. Ultimately, emerging trends, significant knowledge deficiencies, and future research goals are defined to inform the advancement of next-generation MFC systems that support circular economy and net-zero energy initiatives. Full article

Newborn screening in England is a national program with laboratories adhering to common screening algorithms. Until recently, screening for inherited metabolic disorders was provided by ten laboratories using laboratory-developed tests (LDTs) and three using commercial assays: harmonization of results proved challenging. Introduction of hereditary tyrosinemia type 1 screening meant LDTs required modification to include the measurement of succinylacetone, and subsequent re-validation. This provided an opportunity to implement a single commercial reagent kit in all laboratories. It was anticipated that this would improve analytical performance and harmonization. This study aimed to determine whether these goals were achieved. Verification across the 13 laboratories revealed that the commercial kit reduced inter-laboratory variation for all analytes demonstrating improved harmonization. However, this was achieved by applying instrument-specific correction factors to all analytes, the magnitude of which were significant, indicating a lack of standardization. Performance of succinylacetone was limited by instrument-dependent background interference from the methionine stable isotope label, underscoring the need to establish evidence-based screening cut-off values (COV) rather than adopting published thresholds. This study emphasizes the need for traceable reference materials to improve laboratory quality and the value of screening outcome data. Full article

African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating swine disease. To date, no commercial ASF vaccine has been authorized for global marketing except in Vietnam, and emerging genotype I/II recombinant ASFV strains pose severe new challenges to ASF control. Live-attenuated vaccines (LAVs) are widely recognized as the most promising strategy for ASF control. This review systematically summarizes three conventional development strategies for ASF LAVs, dissects the molecular mechanisms of two core bottlenecks—intergenotypic ASFV recombination and vaccine strain reversion to virulence—and elaborates rational design strategies for next-generation LAVs based on cutting-edge technologies. These strategies can fundamentally mitigate the aforementioned risks, offering promising solutions for addressing the major limitations of conventional ASF LAVs. Full article

This paper introduces an electrocardiogram (ECG) noise removal front-end amplifier circuit based on a current-feedback operational amplifier (CFOA) that uses the current feedback to detect error signals and control the output. This ECG circuit focuses on denoising the ECG noise to accentuate the ECG electrical signals from the heart. Noises in ECG refer to baseline wander (BW), powerline interference (PLI) and motion artifacts. We proposed a CFOA-based ECG pre-amplifier using the AD844 commercial operational amplifier built inside with a positive second-generation current conveyor (CCII+) and a voltage follower circuit. This work introduces an ECG noise removal front-end amplifier based on a CFOA. The primary innovation lies in the balancing instrumentation amplifier architecture that utilizes the high-speed and robust properties of the AD844 commercial operational amplifier to achieve superior noise rejection. To protect against high-frequency interference, we introduce a novel cascaded low-pass filter (LPF) stage that ensures a sharper cut-off compared to traditional single-stage designs. Experimental results validate the design’s effectiveness, achieving a high common-mode rejection ratio (CMRR) of 75.4 dB and a mid-band gain of 46.5 dB. These performance metrics, combined with the circuit’s ability to eliminate BW and PLI, confirm its robust suitability for high-fidelity wearable ECG monitoring. Full article

This review provides a comprehensive synthesis of robotic fruit harvesting systems, with a particular focus on the system-level integration of perception, manipulation, and fruit detachment within autonomous harvesting environments. Recent advances in machine vision, deep learning, sensor fusion, robotic end-effectors, grasping strategies, and motion planning are critically analyzed alongside cutting, pulling, and vibration-based detachment mechanisms under unstructured orchard conditions. Beyond component-level analysis, this review emphasizes the critical role of perception–action coupling and highlights key system integration challenges, including localization errors, perception-to-action latency, and environmental variability, which continue to limit reliable field deployment. In addition, orchard and pre-harvest-related factors such as canopy structure, fruit distribution, and detachment force variability are examined in relation to their direct impact on system performance, robustness, and harvesting efficiency. Furthermore, the review extends toward system-level considerations by incorporating performance evaluation metrics, economic feasibility, and scalability constraints, which are essential for transitioning robotic harvesting systems from experimental prototypes to commercially viable solutions, including practical field deployment in distributed and multi-robot harvesting systems. Emerging technologies, including artificial intelligence, advanced sensing, digital agriculture, and energy-aware system design, are discussed as key enablers for achieving adaptive, data-driven, and scalable autonomous harvesting. The novelty of this work lies in proposing an integrated framework that explicitly links perception, manipulation, and detachment with orchard-level constraints and deployment requirements, thereby bridging the gap between algorithmic advancements and real-world implementation of autonomous fruit harvesting systems. Full article