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21 pages, 26631 KiB  
Technical Note
Induced Polarization Imaging: A Geophysical Tool for the Identification of Unmarked Graves
by Matthias Steiner and Adrián Flores Orozco
Remote Sens. 2025, 17(15), 2687; https://doi.org/10.3390/rs17152687 - 3 Aug 2025
Viewed by 207
Abstract
The identification of unmarked graves is important in archaeology, forensics, and cemetery management, but invasive methods are often restricted due to ethical or cultural concerns. This necessitates the use of non-invasive geophysical techniques. Our study demonstrates the potential of induced polarization (IP) imaging [...] Read more.
The identification of unmarked graves is important in archaeology, forensics, and cemetery management, but invasive methods are often restricted due to ethical or cultural concerns. This necessitates the use of non-invasive geophysical techniques. Our study demonstrates the potential of induced polarization (IP) imaging as a non-invasive remote sensing technique specifically suited for detecting and characterizing unmarked graves. IP leverages changes in the electrical properties of soil and pore water, influenced by the accumulation of organic matter from decomposition processes. Measurements were conducted at an inactive cemetery using non-invasive textile electrodes to map a documented grave from the early 1990s, with a survey design optimized for high spatial resolution. The results reveal a distinct polarizable anomaly at a 0.75–1.0 m depth with phase shifts exceeding 12 mrad, attributed to organic carbon from wooden burial boxes, and a plume-shaped conductive anomaly indicating the migration of dissolved organic matter. While electrical conductivity alone yielded diffuse grave boundaries, the polarization response sharply delineated the grave, aligning with photographic documentation. These findings underscore the value of IP imaging as a non-invasive, data-driven approach for the accurate localization and characterization of graves. The methodology presented here offers a promising new tool for archaeological prospection and forensic search operations, expanding the geophysical toolkit available for remote sensing in culturally and legally sensitive contexts. Full article
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27 pages, 9112 KiB  
Article
Impact of Urban Green Spaces on the Livelihoods of Residents in Bulawayo and Johannesburg Cities
by Shepard Nyamambi Maphosa, Sellina Ennie Nkosi and Yingisani Chabalala
Urban Sci. 2025, 9(6), 194; https://doi.org/10.3390/urbansci9060194 - 28 May 2025
Viewed by 1261
Abstract
Urban green spaces (UGSs) play a pivotal role in sustaining the livelihoods of urban dwellers. This study sought to explore the impact of UGSs on livelihoods in Bulawayo and Johannesburg cities. A mixed-methods approach was used to develop a nuanced understanding of the [...] Read more.
Urban green spaces (UGSs) play a pivotal role in sustaining the livelihoods of urban dwellers. This study sought to explore the impact of UGSs on livelihoods in Bulawayo and Johannesburg cities. A mixed-methods approach was used to develop a nuanced understanding of the nexus between UGSs and the livelihoods of the residents. A questionnaire survey (n = 658) with 329 participants from each city and 20 interviews were used to gather and generate data. Twelve types of UGSs were identified, with a relatively large proportion of the participants recognizing informal recreational areas as the common type of urban green space (UGS) in both cities. Domestic gardens, cemeteries, parks, woodlands, institutional green spaces, street trees, wastelands, commonages, and green roofs were other green spaces in both cities. Economically, job opportunities emerged in areas such as selling wares, photography, and the collection of firewood and wild fruits for sale. Likewise, farming activities and property values increased. Socially, they were valuable recreation and leisure spots for picnicking, dog walking, dating escapades, mental and spiritual wellness as well as education. Environmentally, UGSs were special in terms of medicinal provisions and aesthetics. However, urbanization and encroachment are undermining the extent of livelihood benefits. Therefore, it is imperative to revitalize UGSs by instituting robust partnerships and collaboration between government agencies, mobilize resources and expertise, value addition to existing UGSs, rigorous education to promote better appreciation, inclusion of the locals in the design process so that green spaces meet their needs and priorities, and establishing effective maintenance and management systems that ensure sustainability of UGSs. Full article
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20 pages, 5482 KiB  
Article
Temporal Activity and Distribution of the Invasive Mosquitoes Aedes albopictus and Aedes japonicus in the Zagreb Area, Croatia
by Ana Klobučar, Mihaela Kavran, Sunčica Petrinić and Marcela Curman Posavec
Trop. Med. Infect. Dis. 2024, 9(11), 263; https://doi.org/10.3390/tropicalmed9110263 - 4 Nov 2024
Cited by 1 | Viewed by 1882
Abstract
Aedes albopictus and Aedes japonicus are invasive mosquito species that are causing great public concern. Aedes albopictus have successfully spread in Croatia, established in both the coastal and continental parts of the country, while Aedes japonicus is invading temperate climate areas. The invasive [...] Read more.
Aedes albopictus and Aedes japonicus are invasive mosquito species that are causing great public concern. Aedes albopictus have successfully spread in Croatia, established in both the coastal and continental parts of the country, while Aedes japonicus is invading temperate climate areas. The invasive Aedes species are particularly attracted to the black plastic water containers and flower vases in cemeteries where they oviposit their eggs. Therefore, monitoring of this species was carried out in 12 cemeteries in Zagreb, using ovitraps with masonite strips as a substrate for oviposition. The monitoring was carried out from 2017 to 2020. The traps were inspected and the substrate was replaced every two weeks. This study showed that these two invasive species were present and very abundant in the cemeteries. In the case of Ae. albopictus, a higher population density and a greater number of occupied cemeteries were detected. This species was identified in all 12 cemeteries. Aedes albopictus was identified as the dominant species at all study sites. The spread of Ae. japonicus increased during 2018 in comparison to the previous year. Although this species was present in approximately 9% of the ovitraps, the observed population remained consistent throughout the course of the study. The findings indicate that cemeteries can be considered as significant public health hotspots, as the invasive Aedes mosquitoes tend to develop and reproduce in such environments. Consequently, the mosquito population of these two invasive species may only be reduced by applying integrated mosquito management measures, focused on the education of citizens. Full article
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17 pages, 1455 KiB  
Article
Determination of Soil Contamination Due to the Influence of Cemeteries for the Surrounding Land and People in Central Ecuador—Worldwide Implications
by Viviana Abad-Sarango, Tania Crisanto-Perrazo, Paulina Guevara-García, Greta Fierro-Naranjo, Theofilos Toulkeridis, Edwin Ocaña Garzón, Betzabeth Quishpe-Gómez and Silvana Suntaxi-Pachacama
Land 2024, 13(8), 1306; https://doi.org/10.3390/land13081306 - 17 Aug 2024
Viewed by 1863
Abstract
Human decomposition processes generate pulses of nutrients, such as carbon (C) and nitrogen (N) in the form of ammonium and nitrate (NO3), which are released into the surrounding environment. The little exploration related to the potential of cadaveric leachate to [...] Read more.
Human decomposition processes generate pulses of nutrients, such as carbon (C) and nitrogen (N) in the form of ammonium and nitrate (NO3), which are released into the surrounding environment. The little exploration related to the potential of cadaveric leachate to influence the physical chemistry and biology of the soil makes it difficult to obtain data and scientific evidence, and subsequently the predominant objective of the current study was to determine soil contamination through the analysis of parameters of physical chemistry that included organic matter (OM), NO3, texture, humidity, and pH. Soil samples were taken at different depths in two temporary trials (the dry and rainy seasons) in central Ecuador. The Kruskal–Wallace and ANOVA statistical analyses determined significant differences in relation to the sampling sections and by categories, while there were no significant differences in the inter-season analysis; therefore, the study was based on the data obtained in the dry season. The results indicate a tendency towards contamination in cemeteries categorized as critical, that is, moderate, light, and not suitable due to the high values of OM and humidity measured. On the contrary, the soils that corresponded to the cemeteries classified as suitable yielded low values of the analyzed parameters, which corroborates their capacity for the present and future location of cemeteries. Monitoring and managing soil health is crucial to ensure sustainable environmental practices and protect public health; nonetheless, additional research is suggested to confirm the findings of the current study. Full article
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15 pages, 11374 KiB  
Article
Colonial Ascidian Populations at Inshore Coral Reefs of Norfolk Island, South Pacific
by Shannon Eckhardt, Tracy D. Ainsworth, William Leggat and Charlotte E. Page
Diversity 2024, 16(7), 384; https://doi.org/10.3390/d16070384 - 30 Jun 2024
Viewed by 1558
Abstract
Subtropical coral reefs such as the lagoonal reefs of Norfolk Island in the remote South Pacific are vastly understudied, with many benthic species unrecorded in the scientific literature. Here we report on ascidian populations from 2021 to 2023 at Norfolk Islands inshore reefs. [...] Read more.
Subtropical coral reefs such as the lagoonal reefs of Norfolk Island in the remote South Pacific are vastly understudied, with many benthic species unrecorded in the scientific literature. Here we report on ascidian populations from 2021 to 2023 at Norfolk Islands inshore reefs. Quantitative assessments spanning that period were conducted to assess ascidian presence, cover, and benthic interactions. We show ascidian cover remained persistently low and stable across the lagoonal reefs during the study period. We find adjacent reef site, Cemetery Bay showed variation in ascidian cover over time, where we measure a 3.2-fold increase in cover over 2 years. Ascidians were associated primarily with sand and sediment substrates at all reef locations. Recorded interactions between hard corals and ascidians were low. This study provides valuable insights into the population dynamics of ascidians within subtropical reef ecosystems. Ongoing ascidian population monitoring can provide a comprehensive understanding of ascidian dynamics whilst also providing insight of theimpact of anthropogenic stressors on benthic communities. Together, this can aid in informing management and conservation strategies for subtropical reefs. Full article
(This article belongs to the Special Issue Patterns Of Marine Benthic Biodiversity)
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15 pages, 3821 KiB  
Article
Chloroplast Spacer DNA Analysis Revealed Insights into Phylogeographical Structure of Phoebe chekiangensis
by Xiankun Wu, Yan Chen, Chenhui Nan, Shucheng Gao, Xiangzhen Chen and Xiangui Yi
Forests 2024, 15(7), 1073; https://doi.org/10.3390/f15071073 - 21 Jun 2024
Cited by 1 | Viewed by 1213
Abstract
Research studies on the conservation genetics of endangered plants play a crucial role in establishing management plans for biodiversity conservation. Phoebe chekiangensis is a precious and scarce tree species resource in the East China region. To comprehend the origin, evolutionary history, geographical, and [...] Read more.
Research studies on the conservation genetics of endangered plants play a crucial role in establishing management plans for biodiversity conservation. Phoebe chekiangensis is a precious and scarce tree species resource in the East China region. To comprehend the origin, evolutionary history, geographical, and historical factors that has contributed to the current distribution pattern of Phoebe chekiangensis in the East China region, we conducted a phylogeographic analysis that utilized intergenic spacers of chloroplast DNA (cpDNA). We amplified and sequenced three spacer regions of cpDNA (psbC-trnS, trnL-Intro, and Ycf3) intergenic spacer regions of 306 individuals from 11 populations, encompassing the majority of its geographical range in China. Our analysis revealed a total of 11 haplotypes. The research findings show that the spacer regions of the cpDNA genetic diversity of Phoebe chekiangensis was Hd = 0.423, and the nucleotide diversity was Pi × 10−3 = 0.400. At the species level, the population differentiation index Fst = 0.25610 (p < 0.05), and the gene flow Nm = 0.73. The genetic variation between populations was 29.14%, while within populations, it was 70.86%, with the inter-population genetic variation much lower than the within-population variation. The divergence time between the genera Phoebe and Machilus was estimated to be approximately 37.87 mya (PP = 1; 95%HPD: 25.63–44.54 mya), and the crown group time of the genus Phoebe was estimated to be 21.30 mya (PP = 1; 95%HPD: 9.76–34.94 mya). The common ancestor of the 11 Phoebe chekiangensis haplotypes was 7.85 mya, while the H7, H8, and H10 haplotypes of Phoebe chekiangensis (northern region) differentiated relatively late, with a divergence time of 1.90 mya. Neutrality tests (NTs) and mismatch distribution analysis (MDA) suggest that the time frame for Phoebe chekiangensis to expand southwestward along Wuyishan was relatively short and its adaptability to the environment was low, thereby limiting the formation of new haplotypes. These results suggest that Phoebe chekiangensis exhibited greater adaptation to the northern subtropics than to the central subtropics, offering valuable insights for the conservation and utilization of germplasm resources. Full article
(This article belongs to the Section Genetics and Molecular Biology)
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18 pages, 4566 KiB  
Article
Precision Forestry Versus Non-Precision Archaeology—Integrating Forest Management and Archaeological Site Protection
by Johanna Roiha, Topi Tanhuanpää, Juha Hyyppä and Markus Holopainen
Forests 2024, 15(6), 918; https://doi.org/10.3390/f15060918 - 24 May 2024
Cited by 1 | Viewed by 1361
Abstract
This study examines the precision of spatial data required for archaeological sites in Finland from a forest management perspective, identifies archaeological sites at risk due to forestry activities, and investigates potential collaboration between archaeology and forestry to enhance the preservation and understanding of [...] Read more.
This study examines the precision of spatial data required for archaeological sites in Finland from a forest management perspective, identifies archaeological sites at risk due to forestry activities, and investigates potential collaboration between archaeology and forestry to enhance the preservation and understanding of these sites. Due to outdated methods and the lack of systematic field surveys, challenges in achieving accurate spatial data for archaeological sites are highlighted through a literature review, case studies, and fieldwork. Our findings indicate that a significant portion of sites lack boundary delineation, exposing them to the risk of destruction. Moreover, the study reveals limited collaboration between the archaeology and forestry sectors, despite mutual interests in site preservation. We advocate for improved spatial data accuracy using modern technological methods like precision GPS and enhanced cooperation between archaeologists and forestry professionals. This approach aims to protect Finland’s archaeological heritage while promoting responsible forestry practices. Notably, fieldwork enabled the precise determination of the location of Finland’s first separate plague cemetery, underscoring the value of direct field verification in enriching historical and archaeological understanding. Full article
(This article belongs to the Section Forest Ecology and Management)
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32 pages, 14379 KiB  
Article
Acoustic Characterization of Potential Quiet Areas in Dortmund, Germany
by Bryce T. Lawrence, Andreas Frücht, Damian Heying, Kai Schröer and Dietwald Gruehn
Environments 2024, 11(4), 69; https://doi.org/10.3390/environments11040069 - 31 Mar 2024
Cited by 3 | Viewed by 2689
Abstract
German noise action plans aim to reduce negative health outcomes from noise exposure and identify quiet areas free of noise pollution. Quiet area identification in German noise action plans is based primarily on noise mapping and spatial analysis and not empirical or qualitative [...] Read more.
German noise action plans aim to reduce negative health outcomes from noise exposure and identify quiet areas free of noise pollution. Quiet area identification in German noise action plans is based primarily on noise mapping and spatial analysis and not empirical or qualitative data about acoustic environments, thus leaving a gap in the understanding of the quality of formally recognized quiet areas in noise action plans. This work presents a comparative empirical case study in Dortmund, Germany, with the aim to describe the diurnal dB(A) and biophonic properties of quiet areas versus noise ‘hot spots’. Sound observations (n = 282,764) were collected in five different natural or recreational land use patch types larger than four acres within 33 proposed quiet areas in Dortmund (n = 70) and 23 noise hot spots between 27 April 2022 and 2 March 2023. We found that quiet areas are on average more than 20 dB(A) quieter than noise hot spots almost every hour of the day. Forests, managed tree stands, cemeteries, and agriculture diel patterns are dominated by dawn dusk chorus in spring and summer, whereas sports and recreation as well as noise hot spots are dominated by traffic and human noise. A novel composite biophony mapping procedure is presented that finds distinct temporal distribution of biophony in forested and agriculture peri-urban locations positively associated with patch size, distance away from LDEN > 55, proximity to water, and the number of vegetation layers in the plant community. Anthrophony distribution dominates urban land uses in all hours of the day but expands during the day and evening and contracts at night and in dusk hours. The procedures presented here illustrate how qualitative information regarding quiet areas can be integrated into German noise action planning. Full article
(This article belongs to the Special Issue New Solutions Mitigating Environmental Noise Pollution II)
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19 pages, 693 KiB  
Article
Could Necroleachate Be the Cemetery’s Sewage? A Panorama from Brazilian Legislation
by Ana Paula Chein Bueno de Azevedo, Telma Abdalla de Oliveira Cardoso and Simone Cynamon Cohen
Int. J. Environ. Res. Public Health 2023, 20(19), 6898; https://doi.org/10.3390/ijerph20196898 - 8 Oct 2023
Cited by 3 | Viewed by 2609
Abstract
Cemeteries can be compared to landfills, as the leachate produced in these areas, also known as necroleachate, can be environmentally transported, polluting groundwater, surface water, and soil. In Brazil, to ensure no negative environmental impacts and public health risks, cemetery management is the [...] Read more.
Cemeteries can be compared to landfills, as the leachate produced in these areas, also known as necroleachate, can be environmentally transported, polluting groundwater, surface water, and soil. In Brazil, to ensure no negative environmental impacts and public health risks, cemetery management is the responsibility of states and municipalities. In this context, this article aims to discuss Brazilian sanitary–environmental legislation concerning cemetery waste management. Only half of all Brazilian states have established sanitary–environmental cemetery legislation, and only 19 municipalities have specific laws. These laws, however, are broad and contain many gaps. Necroleachate care and control require both sanitary and environmental assessments to avoid environmental vulnerability and contamination risks for populations inhabiting surrounding areas. In this regard, new water analysis parameters in environmentally vulnerable areas should be established to control the population’s drinking water quality, such as the detection of C. perfringens. Furthermore, the construction of vertical cemeteries instead of horizontal ones and the adoption of cremation procedures should also be considered. This assessment comprises a novel research framework, as no studies on the impact of Brazilian laws on environmental necroleachate contamination are available to date. Full article
(This article belongs to the Special Issue Wastewater Treatment and Environmental Monitoring)
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24 pages, 5552 KiB  
Article
Assessment of Cultural Ecosystem Services and Well-Being: Testing a Method for Evaluating Natural Environment and Contact Types in the Harku Municipality, Estonia
by Fiona Nevzati, Mart Külvik, Joanna Storie, Liisa-Maria Tiidu and Simon Bell
Sustainability 2023, 15(13), 10214; https://doi.org/10.3390/su151310214 - 27 Jun 2023
Cited by 6 | Viewed by 4151
Abstract
This study examined the evaluation of cultural ecosystem services (CESs) and their impact on well-being in peri-urban areas, using a case study in Harku municipality, Estonia. CESs, encompassing intangible factors such as emotions and values, are crucial for well-being but challenging to assess. [...] Read more.
This study examined the evaluation of cultural ecosystem services (CESs) and their impact on well-being in peri-urban areas, using a case study in Harku municipality, Estonia. CESs, encompassing intangible factors such as emotions and values, are crucial for well-being but challenging to assess. To address this, a pilot method was developed, involving a typology of natural environment types (NETs) and contact types (CTs), assessed by a panel of local experts. The results revealed that “spiritual, historic, and symbolic” gardens exhibited a strong positive connection to well-being. Blue and green spaces offering physical activities and aesthetics were also highly rated. Surprisingly, cemeteries scored higher than expected. Agreement among experts varied, with “parks + sporting” showing near-perfect consensus and weaker agreement found in “parks + food production”, “blue spaces + providing gathering places”, and “green landscape elements + education”, highlighting diverse expert perspectives in identifying suitable combinations of NETs and CTs. This study addresses research-to-practice gaps and methodological challenges in applying CESs within planning frameworks, providing valuable insights for managing and conserving services in peri-urban areas. By testing the proposed method, this research contributes to a better understanding of how CESs can be effectively integrated into planning processes, fostering sustainable well-being in peri-urbanised regions. Full article
(This article belongs to the Special Issue Forest Ecosystem Services and Urban Green Space)
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23 pages, 13749 KiB  
Article
Groundwater Vulnerability Assessment to Cemeteries Pollution through GIS-Based DRASTIC Index
by Vanessa Gonçalves, Antonio Albuquerque, Paulo Carvalho, Pedro Almeida and Victor Cavaleiro
Water 2023, 15(4), 812; https://doi.org/10.3390/w15040812 - 19 Feb 2023
Cited by 19 | Viewed by 6669
Abstract
Deposition of corpses in the ground is the most common burial practice, which can allow interactions between polluting compounds and the soil, groundwater, and surface water, which may afterwards lead to negative environmental impacts and risks to public health. The risk of cemeteries [...] Read more.
Deposition of corpses in the ground is the most common burial practice, which can allow interactions between polluting compounds and the soil, groundwater, and surface water, which may afterwards lead to negative environmental impacts and risks to public health. The risk of cemeteries contaminating groundwater is related to their location, the quantity of clothes, metals and adornments buried, and geographical, geological, hydrogeological, and climatic factors. Using the DRASTIC index and geographical information system (GIS) tools, the potential for groundwater contamination was investigated in eight cemeteries located in the Figueira da Foz region (Portugal), which are the main anthropogenic pollution sources in the area. Aquifer vulnerability was assessed through the development of thirteen site characteristic maps, seven thematic maps, and a DRASTIC index vulnerability map, using GIS operation tools. No studies were found on the development of vulnerability maps with this method and digital tools. Cemeteries UC2, UC4, UC5, UC6, UC7, and UC8 are located within the zones susceptible to recharge, with an average recharge rate of 254 mm/year. Cemeteries UC5, UC7, and UC8 are expected to develop a greater water-holding capacity. The water table depth is more vulnerable at UC6, varying between 9.1 m and 15.2 m. However, results show only a high vulnerability associated with the UC4 cemetery with the contributions T,C > R,S > I > A > D, which should be under an environmental monitoring program. The area surrounding UC4 is characterized by a water table depth ranging between 15.2 m to 22.9 m, mainly fine-grained sands in both the vadose zone and the aquifer media, Gleyic Solonchaks at the topsoil, very unfavorable slope (0–2%), and high hydraulic conductivity (>81.5 m/day). The sensitivity analysis shows that the topography, soil media, and aquifer media weights were the most effective in the vulnerability assessment. However, the highest contributions to index variation were made by hydraulic conductivity, net recharge, and soil media. This type of approach not only makes it possible to assess the vulnerability of groundwater to contamination from cemeteries but also allows the definition of environmental monitoring plans as well as provides the entities responsible for its management and surveillance with a methodology and tools for its continuous monitoring. Full article
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15 pages, 1082 KiB  
Perspective
The Importance of Soil on Human Taphonomy and Management of Portuguese Public Cemeteries
by Angela Silva-Bessa, Áurea Madureira-Carvalho, Lorna Dawson, Maria Teresa Ferreira, Ricardo Jorge Dinis-Oliveira and Shari L. Forbes
Forensic Sci. 2022, 2(4), 635-649; https://doi.org/10.3390/forensicsci2040047 - 29 Sep 2022
Cited by 9 | Viewed by 6520
Abstract
Cemeteries from the European Romantic period (18th–19th centuries) are often compared to small cities that hold memories, art, and history. Portuguese public cemeteries were first established in 1835 and became an interesting combination of fauna, flora, and monumental sculptures to mourn the dead [...] Read more.
Cemeteries from the European Romantic period (18th–19th centuries) are often compared to small cities that hold memories, art, and history. Portuguese public cemeteries were first established in 1835 and became an interesting combination of fauna, flora, and monumental sculptures to mourn the dead at a location outside the limits of the city. Over the past 187 years, laws have been created and amended taking into consideration the needs of the population and the scientific knowledge available at each time point in history. Nevertheless, cemeteries have long been struggling with the lack of burial space which has been emphasised during the two years of the COVID pandemic. This work aims to review the development of Portuguese public cemeteries since their establishment, highlighting the imposed measures for the inhumation and exhumation of the deceased. It will also discuss the importance of soil as an abiotic agent, focusing on eight specific soil properties and their significance on the characterisation of graves. It is expected that a better understanding of the impact of soil on human taphonomy supports the role of city halls in managing public cemeteries, particularly the lack of burial space. Full article
(This article belongs to the Special Issue Feature Papers in Forensic Sciences in 2022)
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21 pages, 2062 KiB  
Article
Urban Cemeteries as Shared Habitats for People and Nature: Reasons for Visit, Comforting Experiences of Nature, and Preferences for Cultural and Natural Features
by Tanja M. Straka, Maren Mischo, Konstantin J. S. Petrick and Ingo Kowarik
Land 2022, 11(8), 1237; https://doi.org/10.3390/land11081237 - 4 Aug 2022
Cited by 11 | Viewed by 7764
Abstract
Cemeteries are globally culturally protected greenspaces in cities that meet different societal needs and often harbor high biodiversity. To harness the potential of cemeteries as urban green infrastructure, stakeholders need to understand why people visit cemeteries and their preferences. We conducted an online [...] Read more.
Cemeteries are globally culturally protected greenspaces in cities that meet different societal needs and often harbor high biodiversity. To harness the potential of cemeteries as urban green infrastructure, stakeholders need to understand why people visit cemeteries and their preferences. We conducted an online survey in Berlin, Germany (n = 627) to understand (i) the reasons for cemetery visits; (ii) preferences for cemetery features; (iii) the effect of a dead tree as a wilderness component on preferences for differently managed green areas (wild, meadows, lawns); (iv) preferences of nature elements as comforting experiences; and (v) how reasons for the visit and sociodemographic variables relate to respondents’ preferences. The major reasons to visit cemeteries were ‘enjoying nature’, ‘mourning’, and ‘historical interest’ and most preferred cemetery features were ‘wildlife‘, ‘solitude’, and ‘vegetation‘. Presenting a dead tree did not modulate preference ratings for green areas that were depicted on photographs. Comforting experiences with nature elements were high overall. The reasons to visit had besides socio-demographic variables predictive potential on pronounced preferences. The results underscore the importance of cemeteries as multidimensional places and indicate tolerance for the inclusion of dead trees as important wildlife habitat. Strategies to develop cemeteries as shared habitats for people and nature should also consider, besides socio-demographic background, the reasons for cemetery visits. Full article
(This article belongs to the Special Issue Feature Papers for Land Planning and Architecture Section)
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15 pages, 1675 KiB  
Article
Six Feet under Microbiota: Microbiologic Contamination and Toxicity Profile in Three Urban Cemeteries from Lisbon, Portugal
by Carla Viegas, Renata Cervantes, Marta Dias, Bianca Gomes, Pedro Pena, Elisabete Carolino, Magdalena Twarużek, Robert Kosicki, Ewelina Soszczyńska, Susana Viegas and Liliana Aranha Caetano
Toxins 2022, 14(5), 348; https://doi.org/10.3390/toxins14050348 - 16 May 2022
Cited by 5 | Viewed by 3015
Abstract
Cemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled [...] Read more.
Cemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled dust, settled dust filters and electrostatic dust cloths—EDC) were employed. The molecular detection of Aspergillus sections and SARS-CoV-2, as well as mycotoxin analysis, screening of azole resistance, and cytotoxicity measurement were also conducted. Total bacteria contamination was 80 CFU·m−2 in settled dust samples, reached 849 CFU·m−2 in EDC and 20,000 CFU·m−2 in swabs, and ranged from 5000 to 10,000 CFU·m−2 in filters. Gram-negative bacteria (VRBA) were only observed in in settled dust samples (2.00 × 105 CFU·m−2). Regarding Aspergillus sp., the highest counts were obtained in DG18 (18.38%) and it was not observed in azole-supplemented SDA media. SARS-CoV-2 and the targeted Aspergillus sections were not detected. Mycophenolic acid was detected in one settled dust sample. Cytotoxic effects were observed for 94.4% filters and 5.6% EDC in A549 lung epithelial cells, and for 50.0% filters and 5.6% EDC in HepG2 cells. Future studies are needed in this occupational setting to implement more focused risk management measures. Full article
(This article belongs to the Special Issue Mycotoxins in Food and Feed—Occurrence and Risk Assessment)
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13 pages, 329 KiB  
Article
Scientific and Ethical Aspects of Identified Skeletal Series: The Case of the Documented Human Osteological Collections of the University of Bologna (Northern Italy)
by Maria Giovanna Belcastro, Annalisa Pietrobelli, Teresa Nicolosi, Marco Milella and Valentina Mariotti
Forensic Sci. 2022, 2(2), 349-361; https://doi.org/10.3390/forensicsci2020025 - 29 Mar 2022
Cited by 19 | Viewed by 5231
Abstract
Osteological collections are an essential source of information on human biological and cultural variability, providing insights about developmental, evolutionary, and biocultural processes. Among osteological series, documented human osteological collections (DHOC) are especially useful due to the opportunity to control biological parameters such as [...] Read more.
Osteological collections are an essential source of information on human biological and cultural variability, providing insights about developmental, evolutionary, and biocultural processes. Among osteological series, documented human osteological collections (DHOC) are especially useful due to the opportunity to control biological parameters such as age-at-death and sex, which are typically unknown in archaeological or forensic cases. Raising ethical concerns about the collection, management, and study of human remains poses anthropologists with renewed responsibilities. These issues become especially pressing when dealing with DHOC. In this contribution, we discuss the scientific value and ethical issues characterizing DHOC using as case study the documented human osteological collections of the University of Bologna. This series includes more than 1000 individuals from Northern Italian and Sardinian cemeteries and is among the largest in Europe. It represents the basis for ongoing research on a large range of methodological studies, especially focused on the reconstruction of biological profile. After outlining the scientific studies performed on this DHOC, we discuss it in the context of the specific legislation featuring the Italian territory. Finally, we highlight some directions where work can be carried out to better balance scientific research, preservation needs, and ethical concerns, stressing the advantages of modern imaging techniques. Full article
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