Search Results (51)

Regional ecological environmental quality (EEQ) is a vital indicator for environmental management and supporting sustainable development. However, the absence of robust and accurate EEQ prediction models has hindered effective environmental strategies. This study proposes a novel approach to address this gap by integrating the ecological index (EI) model with several predictive models, including autoregressive integrated moving average (ARIMA), convolutional neural network (CNN), long short-term memory (LSTM), and cellular automata (CA), to forecast regional EEQ. Initially, the spatiotemporal evolution of the input data used to calculate the EI score was analyzed. Subsequently, tailored prediction models were developed for each dataset. These models were sequentially trained and validated, and their outputs were integrated into the EI model to enhance the accuracy and coherence of the final EEQ predictions. The novelty of this methodology lies not only in integrating existing predictive models but also in employing an innovative fusion technique that significantly improves prediction accuracy. Despite data quality issues in the case study dataset led to higher prediction errors in certain regions, the overall results exhibit a high degree of accuracy. A comparison of long-term EI predictions with EI assessment results reveals that the R² value for the EI score exceeds 0.96, and the kappa value surpasses 0.76 for the EI level, underscoring the robust performance of the integrated model in forecasting regional EEQ. This approach offers valuable insights into exploring regional EEQ trends and future challenges. Full article

Single-cell Assay for Transposase-Accessible Chromatin using sequencing (scATAC-seq) technology enables single-cell resolution analysis of chromatin accessibility, offering critical insights into gene regulation, epigenetic heterogeneity, and cellular differentiation across various biological contexts. However, existing cell annotation methods face notable limitations. Cross-omics approaches, which rely on single-cell RNA sequencing (scRNA-seq) as a reference, often struggle with data alignment due to fundamental differences between transcriptional and chromatin accessibility modalities. Meanwhile, intra-omics methods, which rely solely on scATAC-seq data, are frequently affected by batch effects and fail to fully utilize genomic sequence information for accurate annotation. To address these challenges, we propose scAttG, a novel deep learning framework that integrates graph attention networks (GATs) and convolutional neural networks (CNNs) to capture both chromatin accessibility signals and genomic sequence features. By utilizing the nucleotide sequences corresponding to scATAC-seq peaks, scAttG enhances both the robustness and accuracy of cell-type annotation. Experimental results across multiple scATAC-seq datasets suggest that scAttG generally performs favorably compared to existing methods, showing competitive performance in single-cell chromatin accessibility-based cell-type annotation. Full article

In 5G wireless communication, network slicing is considered one of the key network elements, which aims to provide services with high availability, low latency, maximizing data throughput, and ultra-reliability and save network resources. Due to the exponential expansion of cellular networking in the number of users along with the new applications, delivering the desired Quality of Service (QoS) requires an accurate and fast network slicing mechanism. In this paper, hybrid deep learning (DL) approaches are investigated using convolutional neural networks (CNNs), Long Short-Term Memory (LSTM), recurrent neural networks (RNNs), and Gated Recurrent Units (GRUs) to provide an accurate network slicing model. The proposed hybrid approaches are CNN-LSTM, CNN-RNN, and CNN-GRU, where a CNN is initially used for effective feature extraction and then LSTM, an RNN, and GRUs are utilized to achieve an accurate network slice classification. To optimize the model performance in terms of accuracy and model complexity, the hyperparameters of each algorithm are selected using the Bayesian optimization algorithm. The obtained results illustrate that the optimized hybrid CNN-GRU algorithm provides the best performance in terms of slicing accuracy (99.31%) and low model complexity. Full article

Cell-free massive multiple-input multiple-output (MIMO) networks eliminate cell boundaries and enhance uniform quality of service by enabling cooperative transmission among access points (APs). In conventional cellular networks, user equipment located at the cell edge experiences severe interference and unbalanced resource allocation. However, in cell-free massive MIMO networks, multiple access points cooperatively serve user equipment (UEs), effectively mitigating these issues. Beamforming and cooperative transmission among APs are essential in massive MIMO environments, making efficient power allocation a critical factor in determining overall network performance. In particular, considering power allocation from the central processing unit (CPU) to the APs enables optimal power utilization across the entire network. Traditional power allocation methods such as equal power allocation and max–min power allocation fail to fully exploit the cooperative characteristics of APs, leading to suboptimal network performance. To address this limitation, in this study we propose a convolutional neural network (CNN)-based power allocation model that optimizes both CPU-to-AP power allocation and AP-to-UE power distribution. The proposed model learns the optimal power allocation strategy by utilizing the channel state information, AP-UE distance, interference levels, and signal-to-interference-plus-noise ratio as input features. Simulation results demonstrate that the proposed CNN-based power allocation method significantly improves spectral efficiency compared to conventional power allocation techniques while also enhancing energy efficiency. This confirms that deep learning-based power allocation can effectively enhance network performance in cell-free massive MIMO environments. Full article

Optical coherence tomography (OCT) is a cellular-resolution imaging technique that can be used as non-invasive and real-time imaging and is useful for detecting early stages of diseases. Five in vitro skin cells were measured by the Mirau-based full-field OCT, including keratinocyte (HaCaT cell line), melanocyte, squamous cell carcinoma cell line (A431), and two melanoma cell lines, i.e., A375 and A2058. Deep learning algorithms (particularly convolutional neural networks, CNN) that extract features from images efficiently process the OCT’s complex images. We used four models to classify the images of five types of 2D-OCT skin cells. Based on the ResNet-15 model, the mean accuracy (average accuracy of 10-fold cross-validation) reaches 98.47%, and the standard deviation is only 0.28% with the data augmentation method. Interestingly, while two normal skin cell images mix and the other three cancer skin cell images mix, the model still works to identify normal and cancer cell features. The mean accuracy reaches 96.77%. Furthermore, we used k-fold analysis to detect the model reliability and adopt the Gradient-weighted Class Activation Mapping (GRAD-CAM) to explain the discrimination results. The deep learning algorithm is successfully and efficiently applied to discriminate the OCT skin cell images. Full article

With the growing complexity of skin disorders and the challenges of traditional diagnostic methods, AI offers exciting new solutions that can enhance the accuracy and efficiency of dermatological assessments. Reflectance Confocal Microscopy (RCM) stands out as a non-invasive imaging technique that delivers detailed views of the skin at the cellular level, proving its immense value in dermatology. The manual analysis of RCM images, however, tends to be slow and inconsistent. By combining artificial intelligence (AI) with RCM, this approach introduces a transformative shift toward precise, data-driven dermatopathology, supporting more accurate patient stratification, tailored treatments, and enhanced dermatological care. Advancements in AI are set to revolutionize this process. This paper explores how AI, particularly Convolutional Neural Networks (CNNs), can enhance RCM image analysis, emphasizing machine learning (ML) and deep learning (DL) methods that improve diagnostic accuracy and efficiency. The discussion highlights AI’s role in identifying and classifying skin conditions, offering benefits such as a greater consistency and a reduced strain on healthcare professionals. Furthermore, the paper explores AI integration into dermatological practices, addressing current challenges and future possibilities. The synergy between AI and RCM holds the potential to significantly advance skin disease diagnosis, ultimately leading to better therapeutic personalization and comprehensive dermatological care. Full article

Smartphones with integrated sensors play an important role in people’s lives, and in advanced multi-sensor fusion navigation systems, the use of individual sensor information is crucial. Because of the different environments, the weights of the sensors will be different, which will also affect the method and results of multi-source fusion positioning. Based on the multi-source data from smartphone sensors, this study explores five types of information—Global Navigation Satellite System (GNSS), Inertial Measurement Units (IMUs), cellular networks, optical sensors, and Wi-Fi sensors—characterizing the temporal, spatial, and mathematical statistical features of the data, and it constructs a multi-scale, multi-window, and context-connected scene sensing model to accurately detect the environmental scene in indoor, semi-indoor, outdoor, and semi-outdoor spaces, thus providing a good basis for multi-sensor positioning in a multi-sensor navigation system. Detecting environmental scenes provides an environmental positioning basis for multi-sensor fusion localization. This model is divided into four main parts: multi-sensor-based data mining, a multi-scale convolutional neural network (CNN), a bidirectional long short-term memory (BiLSTM) network combined with contextual information, and a meta-heuristic optimization algorithm. Full article

Ultrasound is a versatile and well-established technique using sound waves with frequencies higher than the upper limit of human hearing. Typically, therapeutic and diagnosis ultrasound operate in the frequency range of 500 kHz to 15 MHz with greater depth of penetration into the body. However, to achieve improved spatial resolution, high-frequency ultrasound (>15 MHz) was recently introduced and has shown promise in various fields such as high-resolution imaging for the morphological features of the eye and skin as well as small animal imaging for drug and gene therapy. In addition, high-frequency ultrasound microbeam stimulation has been demonstrated to manipulate single cells or microparticles for the elucidation of physical and functional characteristics of cells with minimal effect on normal cell physiology and activity. Furthermore, integrating machine learning with high-frequency ultrasound enhances diagnostics, including cell classification, cell deformability estimation, and the diagnosis of diabetes and dysnatremia using convolutional neural networks (CNNs). In this paper, current efforts in the use of high-frequency ultrasound from imaging to stimulation as well as the integration of deep learning are reviewed, and potential biomedical and cellular applications are discussed. Full article

Phosphorylation, a reversible and widespread post-translational modification of proteins, is essential for numerous cellular processes. However, due to technical limitations, large-scale detection of phosphorylation sites, especially those infected by SARS-CoV-2, remains a challenging task. To address this gap, we propose a method called GBMPhos, a novel method that combines convolutional neural networks (CNNs) for extracting local features, gating mechanisms to selectively focus on relevant information, and a bi-directional gated recurrent unit (Bi-GRU) to capture long-range dependencies within protein sequences. GBMPhos leverages a comprehensive set of features, including sequence encoding, physicochemical properties, and structural information, to provide an in-depth analysis of phosphorylation sites. We conducted an extensive comparison of GBMPhos with traditional machine learning algorithms and state-of-the-art methods. Experimental results demonstrate the superiority of GBMPhos over existing methods. The visualization analysis further highlights its effectiveness and efficiency. Additionally, we have established a free web server platform to help researchers explore phosphorylation in SARS-CoV-2 infections. The source code of GBMPhos is publicly available on GitHub. Full article

Pluripotent stem cells can be differentiated into all three germ-layers including ecto-, endo-, and mesoderm in vitro. However, the early identification and rapid characterization of each germ-layer in response to chemical and physical induction of differentiation is limited. This is a long-standing issue for rapid and high-throughput screening to determine lineage specification efficiency. Here, we present deep learning (DL) methodologies for predicting and classifying early mesoderm cells differentiated from embryoid bodies (EBs) based on cellular and nuclear morphologies. Using a transgenic murine embryonic stem cell (mESC) line, namely OGTR1, we validated the upregulation of mesodermal genes (Brachyury (T): DsRed) in cells derived from EBs for the deep learning model training. Cells were classified into mesodermal and non-mesodermal (representing endo- and ectoderm) classes using a convolutional neural network (CNN) model called InceptionV3 which achieved a very high classification accuracy of 97% for phase images and 90% for nuclei images. In addition, we also performed image segmentation using an Attention U-Net CNN and obtained a mean intersection over union of 61% and 69% for phase-contrast and nuclear images, respectively. This work highlights the potential of integrating cell culture, imaging technologies, and deep learning methodologies in identifying lineage specification, thus contributing to the advancements in regenerative medicine. Collectively, our trained deep learning models can predict the mesoderm cells with high accuracy based on cellular and nuclear morphologies. Full article

Oral potentially malignant disorders (OPMDs) are precursors to over 80% of oral cancers. Hematoxylin and eosin (H&E) staining, followed by pathologist interpretation of tissue and cellular morphology, is the current gold standard for diagnosis. However, this method is qualitative, can result in errors during the multi-step diagnostic process, and results may have significant inter-observer variability. Chemical imaging (CI) offers a promising alternative, wherein label-free imaging is used to record both the morphology and the composition of tissue and artificial intelligence (AI) is used to objectively assign histologic information. Here, we employ quantum cascade laser (QCL)-based discrete frequency infrared (DFIR) chemical imaging to record data from oral tissues. In this proof-of-concept study, we focused on achieving tissue segmentation into three classes (connective tissue, dysplastic epithelium, and normal epithelium) using a convolutional neural network (CNN) applied to three bands of label-free DFIR data with paired darkfield visible imaging. Using pathologist-annotated H&E images as the ground truth, we demonstrate results that are 94.5% accurate with the ground truth using combined information from IR and darkfield microscopy in a deep learning framework. This chemical-imaging-based workflow for OPMD classification has the potential to enhance the efficiency and accuracy of clinical oral precancer diagnosis. Full article

The external environment in the transitional zone of the ecological barrier is fragile, and economic growth has resulted in a series of land degradation issues, significantly impacting regional economic development and the ecological environment. Therefore, monitoring, assessing, and predicting land use changes are crucial for ecological security and sustainable development. This study developed an integrated model comprising convolutional neural network, cellular automata, and Markov chain to forecast the land use status of western Jilin, located in the transitional zone of the ecological barrier, by the year 2030. Additionally, the study evaluated the role of land use policies in the context of land use changes in western Jilin. The findings demonstrate that the coupled modeling approach exhibits excellent predictive performance for land use prediction in western Jilin, yielding a Kappa coefficient of 93.26%. Policy drivers play a significant role in shaping land use patterns in western Jilin, as evidenced by the declining farmland accompanied by improved land utilization, the sustained high levels of forest aligning with sustainable development strategies, the ongoing restoration of waters and grassland, which are expected to show positive growth by 2030, and the steady growth in built-up areas. This study contributes to understanding the dynamics of land use in the transitional zone of the ecological barrier, thereby promoting sustainable development and ecological resilience in the region. Full article

Computerized methods have been developed that allow quantitative morphological analyses of whole slide images (WSIs), e.g., of immunohistochemical stains. The latter are attractive because they can provide high-resolution data on the distribution of proteins in tissue. However, many immunohistochemical results are complex because the protein of interest occurs in multiple locations (in different cells and also extracellularly). We have recently established an artificial intelligence framework, PathoFusion which utilises a bifocal convolutional neural network (BCNN) model for detecting and counting arbitrarily definable morphological structures. We have now complemented this model by adding an attention-based graph neural network (abGCN) for the advanced analysis and automated interpretation of such data. Classical convolutional neural network (CNN) models suffer from limitations when handling global information. In contrast, our abGCN is capable of creating a graph representation of cellular detail from entire WSIs. This abGCN method combines attention learning with visualisation techniques that pinpoint the location of informative cells and highlight cell–cell interactions. We have analysed cellular labelling for CD276, a protein of great interest in cancer immunology and a potential marker of malignant glioma cells/putative glioma stem cells (GSCs). We are especially interested in the relationship between CD276 expression and prognosis. The graphs permit predicting individual patient survival on the basis of GSC community features. Our experiments lay a foundation for the use of the BCNN-abGCN tool chain in automated diagnostic prognostication using immunohistochemically labelled histological slides, but the method is essentially generic and potentially a widely usable tool in medical research and AI based healthcare applications. Full article

Due to the widespread application of neural networks (NNs), and considering the respective advantages of fractional calculus (FC), inertial neural networks (INNs), cellular neural networks (CNNs), and fuzzy neural networks (FNNs), this paper investigates the fixed-time synchronization (FDTS) issues for a particular category of fractional-order cellular-inertial fuzzy neural networks (FCIFNNs) that involve mixed time-varying delays (MTDs), including both discrete and distributed delays. Firstly, we establish an appropriate transformation variable to reformulate FCIFNNs with MTD into a differential first-order system. Then, utilizing the finite-time stability (FETS) theory and Lyapunov functionals (LFs), we establish some new effective criteria for achieving FDTS of the response system (RS) and drive system (DS). Eventually, we offer two numerical examples to display the effectiveness of our proposed synchronization strategies. Moreover, we also demonstrate the benefits of our approach through an application in image encryption. Full article

Barrett’s esophagus (BE) represents a pre-malignant condition characterized by abnormal cellular proliferation in the distal esophagus. A timely and accurate diagnosis of BE is imperative to prevent its progression to esophageal adenocarcinoma, a malignancy associated with a significantly reduced survival rate. In this digital age, deep learning (DL) has emerged as a powerful tool for medical image analysis and diagnostic applications, showcasing vast potential across various medical disciplines. In this comprehensive review, we meticulously assess 33 primary studies employing varied DL techniques, predominantly featuring convolutional neural networks (CNNs), for the diagnosis and understanding of BE. Our primary focus revolves around evaluating the current applications of DL in BE diagnosis, encompassing tasks such as image segmentation and classification, as well as their potential impact and implications in real-world clinical settings. While the applications of DL in BE diagnosis exhibit promising results, they are not without challenges, such as dataset issues and the “black box” nature of models. We discuss these challenges in the concluding section. Essentially, while DL holds tremendous potential to revolutionize BE diagnosis, addressing these challenges is paramount to harnessing its full capacity and ensuring its widespread application in clinical practice. Full article