Search Results (2,249)

This study evaluated the performance, carcass yield, and meat quality of slow-growing chickens fed diets containing different levels of replacement of crude protein from soybean meal with crude protein from cottonseed meal (CSM), with and without supplementation of 2% lysine. A total of 600 male chickens of the Red Colonial heavy strain were used and evaluated from 8 to 56 and to 77 days of age. In Experiment 1, chickens were fed diets with 0, 15, 30, and 45% replacement of soybean meal protein by CSM protein; in Experiment 2, the same replacement levels were associated with supplementation of 2% lysine based on the protein content of CSM. The 15% replacement level promoted higher live weight and average weight gain at 56 and 77 days. Feed conversion ratio at 56 and 77 days indicated that replacing soybean meal protein with CSM protein, with the addition of 2% lysine relative to the protein value, resulted in improved values for this variable. It is concluded that replacing up to 15% of soybean meal protein with cottonseed meal protein, with or without supplementation of 2% lysine, does not compromise productive parameters, carcass yield, or meat quality in slow-growing chickens. Full article

This study evaluated the effects of including palm kernel oil in the diets of pasture-raised sheep on carcass characteristics, meat quality, and fatty acid profiles. A completely randomized design with four treatments was used, consisting of 0, 20, 40, and 60 g/kg of palm kernel oil in the dry matter of the supplement, with eight replicates. Thirty-two uncastrated Santa Inês sheep, with an average initial body weight of 23.2 ± 2.6 kg, were used in this study. The animals were kept on Aruana grass (Panicum maximum (syn. Megathyrsus maximum) cv. Aruana) pastures under continuous stocking for 59 days (preceded by 15 days of adaptation), with each one fed supplements (1.4% of body weight) at 8 am. At the end of the experimental period, the animals were slaughtered in a commercial slaughterhouse for carcass and meat quality evaluation. The inclusion of palm kernel oil had a decreasing linear effect on hot and cold carcass weight (p = 0.0403) (p = 0.0398), but it did not affect hot or cold carcass yields or carcass morphometric measurements, commercial cut weights, pH, or loin area (p > 0.05). However, it affected the color of the L. lumborum muscle, showing an increasing linear effect on yellow intensity (b*) (p = 0.002) and on the centesimal composition, with an increasing linear effect on ether extract content (p = 0.006). Shear force, cooking loss, and water-holding capacity were not affected (p > 0.05). Fatty acid profiles, the atherogenicity and thrombogenicity indices, and the ratio of hypocholesterolemic to hypercholesterolemic fatty acids (h:H) were also unaffected by the inclusion of palm kernel oil (p > 0.05). The inclusion of up to 60 g/kg of palm kernel oil in the diets of pasture-raised sheep had an effect on carcass weight but not yield. It also had an effect on the color and chemical composition of L. lumborum muscle, but these changes did not compromise the overall quality of the meat. Full article

This study aimed to characterize carcass traits, organ weights, reproductive organ measurements, meat chemical composition, and gastrointestinal tract characteristics in Barki (BAR), Rahmani (RAH), and their crossbred BAR × RAH sheep. A total of 30 adult sheep, consisting of 10 animals from each breed group, (5 males and 5 females per group), aged approximately 36.5 ± 0.75 months, were randomly selected from the flock raised under uniform management systems in northern Egypt. The animals were subjected to comprehensive phenotypic evaluation following slaughter. The crossbred BAR × RAH animals were found to be superior for all weight-related traits since they had the highest mean values for cold carcass weight (30.12 ± 2.43 kg), final live body weight (59.46 ± 1.28 kg), slaughter weight (58.27 ± 2.19 kg), as well as trimmed meat weight (23.70 ± 1.35 kg), all of which were significantly higher than those of both purebred breeds (p < 0.001). BAR breed had the lowest values in the weight-related traits but the most favorable ratio of lean-to-fat tissue (4.12 ± 0.92) and the highest content of ash in the meat (1.00 ± 0.05%). Pronounced breed differences were observed in the measurements of the reproductive organs. Chemical analysis of the meat revealed that the meat of the crossbred animals contained the highest amount of moisture (74.66 ± 1.99%) and crude protein (21.65 ± 0.57%). The results of the GI tract revealed that the crossbred animals had the longest intestines (41.17 ± 2.18 m). The pH of the animals also showed breed-specific characteristics. Crossbreeding BAR and RAH improved growth, carcass and reproductive traits, supporting its use in breeding programs. Full article

This study evaluated the effects of sex and genetics on carcass traits and pork quality in 180 carcasses from castrated males and females of three commercial lines: A (Pietrain × Duroc × Large White × Landrace), B (Line A × Pietrain) and C (Line A × Hampshire). Males showed greater backfat (14.05 vs. 13.38%) and lower lean meat (58.65 vs. 59.08%) than females. Line C exhibited the highest marbling score (2.82) and hot and cold carcass weights (90.04 and 88.05 kg), while Line B achieved the highest ham yield (32.5%). Castrated males had higher marbling (2.74 vs. 2.48) but lower shear force (3.68 vs. 3.90 kgf) than females. Line C showed the highest marbling (2.82) and shear force (4.04 kgf). Males of Line C had longer sarcomeres than females of the same line and males from Line B. Males exhibited higher protein (24.73 vs. 23.91%) but lower mineral content (1.55 vs. 1.85%) than females. Line A had the highest mineral content (1.90%). Collagen content was highest in males from Lines A and B (insoluble: 0.35–0.38%; soluble: 0.09%; total: 0.44–0.47%), and lower in Line C (insoluble: 0.18%; soluble: 0.04%; total: 0.21%). These results indicate that sex and new genetic lines influence carcass composition and instrumental meat quality traits. Full article

Yanhuang cattle is a specialized beef breed independently developed in China with high economic value. However, the effects of the horned/polled trait on its production performance and the underlying genetic markers are poorly understood. This study aimed to evaluate the impact of horn status and to validate candidate genetic markers for the polled trait in this breed. A total of 60 Yanhuang cattle (30 horned and 30 polled) were used for comparative analysis of growth, carcass, and meat quality traits. Additionally, blood samples from 200 individuals (100 horned and 100 polled) were collected for molecular screening of seven candidate genetic markers previously associated with polledness in cattle. The polled cattle showed significantly higher body weight from 12 months onward. Body height and hip height were significantly greater at 12, 18, and 24 months, while body length displayed significant advantages at 6, 18, 24, and 30 months. Polled cattle also outperformed in chest girth (18 months), abdominal circumference (significant at 18–30 months), and ischial end width (significant at 6–12 months). Regarding slaughter traits, polled cattle exhibited higher carcass weight, along with significantly improved slaughter and net meat rates, though no differences were observed in meat quality. At the molecular level, the P_202ID indel marker was successfully validated in the polled Yanhuang population. Our findings demonstrate that the polled trait is associated with superior production performance in Yanhuang cattle, and the P_202ID marker represents a reliable molecular tool for polled selection, providing a theoretical and technical basis for the genetic improvement and breeding of this breed. Full article

This study investigated the effects of different breeding methods on the cecal microbiota and production traits of Yimeng Black Goats (YBGs). Twenty-seven 3-month-old male YBGs were assigned to three groups (n = 9 each): total mixed ration once daily (A), concentrate in the morning and roughage in the afternoon (B), or grazing with supplementary feeding (C). Cecal bacterial communities were analyzed via 16S rRNA sequencing, and functional potential was predicted using FAPROTAX. Breeding method significantly altered microbial composition (p < 0.05). Beta diversity was highest in Group C, while alpha diversity remained similar across groups. Bacteroidetes, Firmicutes, and Proteobacteria were dominant; Proteobacteria were most abundant in Group A. At the genus level, relative abundances of nine taxa, including Lactobacillus and Fusobacterium, differed significantly (p < 0.05). At the species level, including Lactobacillus mucosae, Bacteroides massiliensis and Alistipes finegoldii, differed significantly (p < 0.05). Chemoheterotrophy and fermentation functions were most enriched, particularly in Group C. Total weight gain was highest in Group A and lowest in Group C (p < 0.05), while carcass rate showed no significant differences (p > 0.05). Correlation analysis revealed that Proteobacteria, Fusobacteria, and Euryarchaeota as the key phylum, and Bacteroides, Tyzzerella, Fusobacterium, unidentified_Prevotellaceae, Methanovrevibacter and Faecalibacterium as the key genera were influencing the production traits of YBGs. These findings highlight the adaptive responses of the cecal microbiota to breeding methods and their potential links to host performance. Full article

Ross 308 chickens were randomly divided into 2 groups of 600 birds each: a control group (C) and an experimental group (M). The birds were reared for 42 days in accordance with the flock management guidelines. Group M was exposed to music for 2 h/day and for 30 min before slaughter (~70 dB). After slaughter, the carcasses were cooled, and after 24 h, the pectoral muscles were collected for further physicochemical analyses. The study results revealed a lower value of drip loss in the pectoral muscles of the chickens from group M than in those from group C (p ≤ 0.01). Moreover, the muscles from group M chickens had higher pH values at 15 min, 1 h, and 12 h (p ≤ 0.01) and also at 4 h (p ≤ 0.05) post-mortem. Pectoral muscles of group M chickens also showed lower L* and b* color parameters (p ≤ 0.01), collagen content (p ≤ 0.05), and GSH concentration (p ≤ 0.01), compared to control birds. Exposure to ambient relaxation music in the scheme used in the study may be an effective form of environmental enrichment for broiler chickens, leading to physicochemical changes in their pectoral muscles consistent with potentially lower pre-slaughter stress. Full article

Animal welfare monitoring is essential in pig production. On-farm animal welfare (AW) assessments may provide a comprehensive overview but are resource-intensive. Abattoir-based assessments allow pigs from multiple farms to be inspected in a single facility. However, data on the relationship between these assessments remain limited, especially for heavy pigs (160–170 kg). This study investigates these associations in Italian heavy pig production. At the abattoir, 18,333 pig carcasses from 185 batches across 86 farms were scored for tail, skin (cranial and caudal) and ear lesions. On-farm AW assessments (management, structures and animal-based measures) were obtained from the national surveillance system (ClassyFarm). Tail lesion scores were higher in pigs with intact tails, whereas ear scores showed the opposite trend, suggesting a substitution effect between tail and ear biting. This indicates that tail docking is insufficient to fully prevent abnormal behaviours. Higher skin and ear scores were associated with suboptimal management, but tail scores were not, likely due to the multifactorial nature of tail biting. Herd size had no significant effect on welfare indicators. These results highlight the complexity of assessing AW and the importance of combining abattoir and farm data to obtain a more integrated monitoring system. Full article

This study evaluated the effects of dietary spirulina (Spirulina platensis) supplementation on the physiological status and bone quality of juvenile ring-necked pheasants. A total of 200 one-day-old chicks were randomly allocated to a control group fed a basal diet or to an experimental group fed a basal diet supplemented with 15 g of spirulina/kg feed and reared under identical conditions for 42 days. Growth performance, feed intake, the feed conversion ratio, selected carcass traits, meat and bone quality, and biochemical and hematological parameters were assessed. Pheasants receiving spirulina presented significantly greater body weights at four weeks of age. The experimental group also exhibited increased pH values in pectoral and thigh muscles and increased postmortem muscle temperature. Spirulina supplementation significantly improved tibia quality, as reflected by enhanced geometric, structural, and material bone properties. The birds fed spirulina presented increased plasma total cholesterol and low-density lipoprotein concentrations, whereas those in the control group exhibited increased malondialdehyde levels, indicating increased oxidative stress. In addition, spirulina increased the proportion of lymphocytes and reduced both the proportion and absolute number of monocytes. In conclusion, spirulina supplementation increased tibia bone strength, reduced oxidative stress, and had immunomodulatory effects on juvenile pheasants. Full article

This study evaluated the effects of forage-free diets with reduced starch levels on the productive performance, metabolism, ruminal fermentation, nutrient digestibility, and meat quality of feedlot beef cattle. Two experiments were conducted. In Experiment 1, forty uncastrated Nellore steers were distributed into 20 pens in a completely randomized design, receiving diets with increasing inclusion levels of ground corn in the total diet: C400 (400 g kg⁻¹), C200 (200 g kg⁻¹), C100 (100 g kg⁻¹), and C50 (50 g kg⁻¹), formulated without forage and based on fibrous co-products. Increasing ground corn inclusion promoted linear improvements in final body weight and average daily gain, while dry matter intake and feed efficiency showed quadratic responses. Meat quality parameters were not affected by dietary treatments. In Experiment 2, eight crossbred steers were assigned to a double 4 × 4 Latin square design and fed the same experimental diets. Higher corn inclusion increased starch and fat intake, whereas dry matter, organic matter, and protein intake showed quadratic responses. Apparent total-tract digestibility of dry matter, organic matter, and starch also followed a quadratic pattern. Ruminal fermentation parameters were affected by dietary treatments, with greater ammoniacal nitrogen concentrations at higher corn levels and quadratic responses for propionate, butyrate, and methane production. Nitrogen metabolism indicated increased urinary nitrogen and uric acid excretion with increasing dietary corn inclusion. These results demonstrate that forage-free diets based on citrus pulp and soybean hulls with different levels of ground corn can be effectively used in finishing beef cattle, improving performance without impairing meat quality while modulating ruminal fermentation and nutrient utilization. Full article

Wildlife health surveillance is a vital element of disease prevention, biodiversity conservation, and public health protection, especially as most emerging infectious diseases originate from wildlife. In Slovenia, long-term passive surveillance based on necropsy data has yielded valuable insights into wildlife mortality patterns over the past three decades, despite inherent limitations such as carcass detectability, reporting bias, scavenging, and decomposition. Ongoing cooperation among governmental institutions, veterinary services, hunters, and wildlife management organisations has enabled the effective operation of this system, although passive surveillance remains subject to spatial, temporal, and species-specific biases. Necropsy data show that infectious diseases, particularly parasitic infections, are the main causes of mortality in key species such as roe deer and chamois, reflecting both their population abundance and targeted monitoring. In contrast, carcasses of species such as wild boar, red deer, small mammals, and birds are underrepresented due to ecological factors, biosecurity constraints, or low detectability. Overall, while passive wildlife surveillance does not provide representative population-level mortality estimates, it remains a reliable tool for identifying the presence or absence of significant diseases and for understanding broad mortality patterns when interpreted in the context of known methodological and ecological limitations. Full article

Several species of the genus Acinetobacter are nosocomial pathogens with a well-documented ability to acquire resistance to multiple antibiotics. Although Acinetobacter is one of the most abundant genera in meat processing environments, data on this genus outside of clinical environments remains limited. The objective of this study was to ascertain the prevalence, diversity and antimicrobial resistance profile of Acinetobacter spp. in 200 samples collected from food contact surfaces, non-food contact surfaces, carcasses and final meat cuts across five pork, chicken and beef processing facilities, each comprising physically connected slaughterhouses and meat processing plants. Acinetobacter spp. were detected in 80% (95% CI = 71–87%) and 70% (95% CI = 60–79%) of samples from slaughterhouses and processing plants, respectively. The facilities harboured a wide diversity of Acinetobacter species, with 27 different species identified. Acinetobacter baumannii was the species most frequently detected. Whole-genome sequencing of 18 Acinetobacter spp. isolates revealed the presence of ARGs conferring resistance to beta-lactams, tetracyclines and aminoglycosides, and disclosed phylogenetic relationships with isolates from fresh meat. Phenotypic resistance to beta-lactams, fluoroquinolones, aminoglycosides, folate pathway inhibitors and/or tetracyclines was observed in 77.8% of the sequenced isolates, with 44.4% classified as multidrug-resistant. These findings identify meat processing environments as an important reservoir of Acinetobacter spp. and highlight the need for further investigation to prevent the dissemination of antimicrobial-resistant strains. Full article

Actinobacillus pleuropneumoniae is a major swine pathogen that causes pleuropneumonia and leads to substantial economic losses due to mortality, impaired growth, and carcass condemnation. Nineteen serovars have been described, and their geographic distribution has been assessed using multiple typing approaches. High serovar diversity, together with limited cross-protective immunity, increases reliance on antimicrobial therapy for disease control. However, data on the genotypic diversity and antimicrobial susceptibility of A. pleuropneumoniae remain limited worldwide, and information on serovar distribution in Brazil is scarce. Here, we report serotyping, genotyping, and antimicrobial susceptibility profiling of A. pleuropneumoniae isolated from diseased pigs in Brazil. Eighty-five isolates from eight Brazilian states were analyzed; serovars 5 and 10 were the most prevalent (38.8% and 29.4%, respectively). Ceftiofur, spectinomycin, gentamicin, neomycin, tilmicosin, tulathromycin, and florfenicol showed good in vitro activity against the isolates. The highest resistance rates were observed for tylosin (98.8%), clindamycin (90.6%), chlortetracycline (67.1%), and oxytetracycline (67.1%), and multidrug resistance was detected in 55% of strains. SE-AFLP and PFGE revealed high genetic diversity, including among isolates of the same serovar, although a modest tendency to cluster by geographic origin and serovar was observed. Full article

Reducing input costs, especially feed ingredients, remains a priority for production agriculture. Identifying and selecting nutritionally dense ingredients is vital to maximize animal performance. Objectives of the current study were to evaluate the impact of biochar supplementation on goat carcass characteristics and fresh meat quality. Goats (N = 36) were allocated to a diet concentration formulated with or without (Control, Low, Medium, or High g/kg) biochar. After 60 days of feeding, goats were harvested, and carcass measurements were collected. Subprimals from the leg were fabricated into steaks for laboratory analysis of surface color, cook loss, and instrumental tenderness. Biochar supplementation did not alter organ weights (p = 0.0614), dressing percentage (p = 0.8139), loin eye area (p = 0.9570), or tenderness (p = 0.0144). However, marbling scores were lower in goats fed at the medium biochar supplementation rate (p = 0.0114) and high supplementation (p = 0.0102) compared to the control. An interaction between storage day and biochar supplementation was recorded for instrumental surface color lightness (L*; p = 0.0016), redness (a*; p = 0.0547), hue angle (p = 0.0313), and red-to-brown (p = 0.0591). Steaks from the 0.052% supplementation group exhibited greater (p = 0.0003) redness (a*) during a 7-day refrigerated display and increased chroma values (p < 0.0001). Storage duration influenced all color traits, with steak surface discoloration increasing as storage time increased (p < 0.0001). Results conclude that biochar supplementation does not negatively impact all carcass quality or tenderness traits, but may influence fat deposition and improve meat color stability. Full article

This study aimed to assess the effect of concentrate supplementation frequency on the nutrient intake, performance, carcass characteristics, and tissue composition of sheep for meat raised on Panicum maximum cv. Massai pasture. A group of 36 sheep of the Santa Inês breed (18 males and 18 females) with a mean initial weight of 17.0 ± 1.5 kg and mean age of 90 ± 10 days were assigned to three treatments: supplementation every 24 h, every 48 h, and every 72 h. The formulation comprised ground maize kernels (70%), soybean meal (25%), mineral mix (2.5%), urea (1.8%), table salt (0.5%), and ammonium sulfate (0.2%). The females reached a higher final weight than the males (p < 0.05). Dry matter intake did not differ among the treatment groups (p > 0.05), but the sheep supplemented every 72 h showed greater pasture and neutral detergent fiber (NDF) intake, while those supplemented every 24 h and 48 h consumed more concentrate and had higher total weight gain (7.19 and 7.10 × 5.68 kg) (p < 0.05). The same behavior was found for carcass and meat cut weights and for leg and carcass compacity indexes, but carcass and cut yields were not impacted by supplementation strategy. Supplementation on alternate days, i.e., every 48 h, maintains the performance and productive efficiency levels in sheep kept on Massai grass pasture compared to those supplemented daily, but providing intermittent supplementation with intervals above 48 h is not recommended as a nutritional strategy. Full article