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30 pages, 8037 KiB  
Review
A Review of Multiscale Interaction Mechanisms of Wind–Leaf–Droplet Systems in Orchard Spraying
by Yunfei Wang, Zhenlei Zhang, Ruohan Shi, Shiqun Dai, Weidong Jia, Mingxiong Ou, Xiang Dong and Mingde Yan
Sensors 2025, 25(15), 4729; https://doi.org/10.3390/s25154729 (registering DOI) - 31 Jul 2025
Viewed by 20
Abstract
The multiscale interactive system composed of wind, leaves, and droplets serves as a critical dynamic unit in precision orchard spraying. Its coupling mechanisms fundamentally influence pesticide transport pathways, deposition patterns, and drift behavior within crop canopies, forming the foundational basis for achieving intelligent [...] Read more.
The multiscale interactive system composed of wind, leaves, and droplets serves as a critical dynamic unit in precision orchard spraying. Its coupling mechanisms fundamentally influence pesticide transport pathways, deposition patterns, and drift behavior within crop canopies, forming the foundational basis for achieving intelligent and site-specific spraying operations. This review systematically examines the synergistic dynamics across three hierarchical scales: Droplet–leaf surface wetting and adhesion at the microscale; leaf cluster motion responses at the mesoscale; and the modulation of airflow and spray plume diffusion by canopy architecture at the macroscale. Key variables affecting spray performance—such as wind speed and turbulence structure, leaf biomechanical properties, droplet size and electrostatic characteristics, and spatial canopy heterogeneity—are identified and analyzed. Furthermore, current advances in multiscale modeling approaches and their corresponding experimental validation techniques are critically evaluated, along with their practical boundaries of applicability. Results indicate that while substantial progress has been made at individual scales, significant bottlenecks remain in the integration of cross-scale models, real-time acquisition of critical parameters, and the establishment of high-fidelity experimental platforms. Future research should prioritize the development of unified coupling frameworks, the integration of physics-based and data-driven modeling strategies, and the deployment of multimodal sensing technologies for real-time intelligent spray decision-making. These efforts are expected to provide both theoretical foundations and technological support for advancing precision and intelligent orchard spraying systems. Full article
(This article belongs to the Special Issue Application of Sensors Technologies in Agricultural Engineering)
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20 pages, 2144 KiB  
Article
Effects of Crop Load Management on Berry and Wine Composition of Marselan Grapes
by Jianrong Kai, Jing Zhang, Caiyan Wang, Fang Wang, Xiangyu Sun, Tingting Ma, Qian Ge and Zehua Xu
Horticulturae 2025, 11(7), 851; https://doi.org/10.3390/horticulturae11070851 - 18 Jul 2025
Viewed by 368
Abstract
The aim of this study was to investigate the effects of the crop load on the berry and wine composition of Marselan grapes. Thus, the appropriate crop load for Marselan wine grapes in Ningxia was determined based on the shoot density and the [...] Read more.
The aim of this study was to investigate the effects of the crop load on the berry and wine composition of Marselan grapes. Thus, the appropriate crop load for Marselan wine grapes in Ningxia was determined based on the shoot density and the number of clusters per shoot. Marselan grapes from the Gezi Mountain vineyard, located at the eastern foot of Helan Mountain in the Qingtongxia region of Ningxia, were selected as the research material to conduct a combination experiment with four levels of shoot density and three levels of cluster density. The analysis of the berry and wine chemical composition was combined with a wine sensory evaluation to determine the optimal crop load levels. Crop load regulation significantly affected both the grape berry composition and the basic physicochemical properties of the resulting wine. Low crop loads improved metrics such as the berry weight and soluble solids content. A low shoot density facilitated the accumulation of organic acids, flavonols, and hydroxybenzoic acids in wine. Moderate crop loads were conducive to anthocyanin synthesis—the total individual anthocyanins content in the 10–20 shoots per meter of the canopy treatment group ranged from 116% to 490% of the control group—whereas excessive crop loads hindered its accumulation. Crop load management significantly influenced the aroma composition of wine by regulating the content of sugars, nitrogen sources, and organic acids in grape berries, thereby promoting the synthesis of esters and the accumulation of key aromatic compounds, such as terpenes. This process optimized pleasant flavors, including fruity and floral aromas. In contrast, wines from the high crop load and control treatments contained lower levels of these aroma compounds. Compounds such as ethyl caprylate and β-damascenone were identified as potential quality markers. Overall, the wine produced from vines with a crop load of 30 clusters (15 shoots per meter of canopy, 2 clusters per shoot) received the highest sensory scores. Appropriate crop load management is therefore critical to improving the chemical composition of Marselan wine. Full article
(This article belongs to the Section Viticulture)
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20 pages, 3185 KiB  
Article
Radiative Transfer Model-Integrated Approach for Hyperspectral Simulation of Mixed Soil-Vegetation Scenarios and Soil Organic Carbon Estimation
by Asmaa Abdelbaki, Robert Milewski, Mohammadmehdi Saberioon, Katja Berger, José A. M. Demattê and Sabine Chabrillat
Remote Sens. 2025, 17(14), 2355; https://doi.org/10.3390/rs17142355 - 9 Jul 2025
Viewed by 344
Abstract
Soils serve as critical carbon reservoirs, playing an essential role in climate change mitigation and agricultural sustainability. Accurate soil property determination relies on soil spectral reflectance data from Earth observation (EO), but current vegetation models often oversimplify soil conditions. This study introduces a [...] Read more.
Soils serve as critical carbon reservoirs, playing an essential role in climate change mitigation and agricultural sustainability. Accurate soil property determination relies on soil spectral reflectance data from Earth observation (EO), but current vegetation models often oversimplify soil conditions. This study introduces a novel approach that combines radiative transfer models (RTMs) with open-access soil spectral libraries to address this challenge. Focusing on conditions of low soil moisture content (SMC), photosynthetic vegetation (PV), and non-photosynthetic vegetation (NPV), the coupled Marmit–Leaf–Canopy (MLC) model is used to simulate early crop growth stages. The MLC model, which integrates MARMIT and PRO4SAIL2, enables the generation of mixed soil–vegetation scenarios. A simulated EO disturbed soil spectral library (DSSL) was created, significantly expanding the EU LUCAS cropland soil spectral library. A 1D convolutional neural network (1D-CNN) was trained on this database to predict Soil Organic Carbon (SOC) content. The results demonstrated relatively high SOC prediction accuracy compared to previous approaches that rely only on RTMs and/or machine learning approaches. Incorporating soil moisture content significantly improved performance over bare soil alone, yielding an R2 of 0.86 and RMSE of 4.05 g/kg, compared to R2 = 0.71 and RMSE = 6.01 g/kg for bare soil. Adding PV slightly reduced accuracy (R2 = 0.71, RMSE = 6.31 g/kg), while the inclusion of NPV alongside moisture led to modest improvement (R2 = 0.74, RMSE = 5.84 g/kg). The most comprehensive model, incorporating bare soil, SMC, PV, and NPV, achieved a balanced performance (R2 = 0.76, RMSE = 5.49 g/kg), highlighting the importance of accounting for all surface components in SOC estimation. While further validation with additional scenarios and SOC prediction methods is needed, these findings demonstrate, for the first time, using radiative-transfer simulations of mixed vegetation-soil-water environments, that an EO-DSSL approach enhances machine learning-based SOC modeling from EO data, improving SOC mapping accuracy. This innovative framework could significantly improve global-scale SOC predictions, supporting the design of next-generation EO products for more accurate carbon monitoring. Full article
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13 pages, 3041 KiB  
Article
Changes of Plant Growth and Soil Physicochemical Properties by Cultivating Different Economic Plant Species in Saline-Alkali Soil of Hetao Oasis, Inner Mongolia
by Rong Ma, Fengmei Du, Yongli Qin, Jianping Lv, Guanying Xing, Youjie Xu, Na Fu, Jun Qiao, Guangyu Hong and Shaokun Wang
Agriculture 2025, 15(13), 1421; https://doi.org/10.3390/agriculture15131421 - 30 Jun 2025
Viewed by 305
Abstract
Due to prolonged irrigation from the Yellow River, a large area of farmland in the Hetao Oasis has undergone different degrees of salinization and alkalization, leading to reduced crop yields and incapable soil for plant growth. To enhance the productivity of the farmland [...] Read more.
Due to prolonged irrigation from the Yellow River, a large area of farmland in the Hetao Oasis has undergone different degrees of salinization and alkalization, leading to reduced crop yields and incapable soil for plant growth. To enhance the productivity of the farmland with saline-alkali soils, it is important to select salt-tolerant economic plant species that are capable of growing under the local climate and soil conditions in the Hetao Oasis. We conducted the experiment by planting Ziziphus jujuba var. spinose, Elaeagnus angustifolia, Hippophae rhamnoides and Lycium chinense in the Bayan Taohai Farm of the Hetao Oasis. Changes of plant growth (the survival rate, plant height, canopy, basal diameter and new branch length) and soil physicochemical properties (soil organic carbon, total carbon, total nitrogen, pH, electrical conductivity and particle size distribution) were continuously monitored during two growing seasons. Results indicated that, by the end of the first growing season, the survival rate of the Z. jujuba was less than 10%, making it unsuitable for plantation in the saline-alkali soils of the Hetao Oasis. In terms of plant growth, the E. angustifolia exhibited the highest survival rate (94.71%) and the fastest growth rate, indicating that E. angustifolia is adapted in the saline-alkali soils of the Hetao Oasis. The survival rates for L. chinense and H. rhamnoides were 86.46% and 65.64%, respectively, indicating that these species could grow in the saline-alkali soils, but at a slower rate. From the perspective of soil improvement, E. angustifolia, H. rhamnoides and L. chinense could reduce the soil pH, and E. angustifolia could significantly increase soil nutrients. In conclusion, it is not recommended to plant Z. jujuba, while the E. angustifolia is recommended as a proper economic species to be widely planted in the saline-alkali soils of the Hetao Oasis. H. rhamnoides could be selectively planted in areas with better soil conditions, and the L. chinense could be planted following soil improvement measurements. The research enhanced the effective utilization of the saline-alkali farmland and provided proper economic plant species for sustainable agriculture management in the Hetao Oasis of Inner Mongolia. Full article
(This article belongs to the Special Issue Soil Microbial Community and Ecological Function in Agriculture)
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16 pages, 9522 KiB  
Article
Tabonuco and Plantation Forests at Higher Elevations Are More Vulnerable to Hurricane Damage and Slower to Recover in Southeastern Puerto Rico
by Michael W. Caslin, Madhusudan Katti, Stacy A. C. Nelson and Thrity Vakil
Land 2025, 14(7), 1324; https://doi.org/10.3390/land14071324 - 21 Jun 2025
Viewed by 1394
Abstract
Hurricanes are major drivers of forest structure in the Caribbean. In 2017, Hurricane Maria caused substantial damage to Puerto Rico’s forests. We studied forest structure variation across 75 sites at Las Casas de la Selva, a sustainable forest plantation in Patillas, Puerto Rico, [...] Read more.
Hurricanes are major drivers of forest structure in the Caribbean. In 2017, Hurricane Maria caused substantial damage to Puerto Rico’s forests. We studied forest structure variation across 75 sites at Las Casas de la Selva, a sustainable forest plantation in Patillas, Puerto Rico, seven years after Hurricane Maria hit the property. At each site we analyzed 360° photos in a 3D VR headset to quantify the vertical structure and transformed them into hemispherical images to quantify canopy closure and ground cover. We also computed the Vertical Habitat Diversity Index (VHDI) from the amount of foliage in four strata: herbaceous, shrub, understory, and canopy. Using the Local Bivariate Relationship tool in ArcGIS Pro, we analyzed the relationship between forest recovery (vertical structure, canopy closure, and ground cover) and damage. Likewise, we analyzed the effects of elevation, slope, and aspect, on damage, canopy closure, and vertical forest structure. We found that canopy closure decreases with increasing elevation and increases with the amount of damage. Higher elevations show a greater amount of damage even seven years post hurricane. We conclude that trees in the mixed tabonuco/plantation forest are more susceptible to hurricanes at higher elevations. The results have implications for plantation forest management under climate-change-driven higher intensity hurricane regimes. Full article
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17 pages, 1669 KiB  
Article
Assessment of Wind-Related Parameters and Erodibility Potential Under Winter Wheat Canopy in Reclaimed Tidal Flat Land
by Kyosuk Lee, Jaehan Lee, Kwangseung Lee, Hyunsuk Jo, Woojung Choi, Jinwoong Cho and Dougyoung Chung
Agronomy 2025, 15(7), 1504; https://doi.org/10.3390/agronomy15071504 - 20 Jun 2025
Viewed by 407
Abstract
The aim of this study was to observe soil erosion by wind, depending on the soil physical properties, climatic conditions, and plant canopy, for three representative soil series in the reclaimed tidal flats. Soil samples were collected from the Ap horizon of three [...] Read more.
The aim of this study was to observe soil erosion by wind, depending on the soil physical properties, climatic conditions, and plant canopy, for three representative soil series in the reclaimed tidal flats. Soil samples were collected from the Ap horizon of three soil series to analyze soil physical properties and particle distribution. Precipitation and wind velocities were measured by the weather station installed at the filed. The particle distribution curves showed that the actual proportions of erodible soil particle were in the order of 74.7%(TH), 66.1%(PS), and 62%(JB). The instantaneous and daily maximum wind speeds exceeded the threshold friction velocity (5.78 m s−1) suggested by Chepil. However, the dynamic velocities, depending on the radius of 0.125 mm and 0.42 mm belonging to erodible particle size, were much lower than the threshold friction velocity suggested by Chepil. The wind profile increases logarithmically with height, just above the plant canopy. The vertical gradients of wind velocity for the winter wheat plot were smaller than that of the bare plot due to the relatively rough canopy, and U(Z)c of the bare plot was slightly higher than that of the winter wheat plot with a plant canopy for the given U(Z)m. Conclusively, the actual proportion of erodible particles was much less than that of the particle size limit. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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16 pages, 3461 KiB  
Article
Investigating the Influence of the Weed Layer on Crop Canopy Reflectance and LAI Inversion Using Simulations and Measurements in a Sugarcane Field
by Longxia Qiu, Xiangqi Ke, Xiyue Sun, Yanzi Lu, Shengwei Shi and Weiwei Liu
Remote Sens. 2025, 17(12), 2014; https://doi.org/10.3390/rs17122014 - 11 Jun 2025
Viewed by 322
Abstract
Recent research in agricultural remote sensing mainly focuses on how soil background affects canopy reflectance and the inversion of LAI, while often overlooking the influence of the weed layer. The coexistence of crop and weed layers forms two-layered vegetation canopies in tall crops [...] Read more.
Recent research in agricultural remote sensing mainly focuses on how soil background affects canopy reflectance and the inversion of LAI, while often overlooking the influence of the weed layer. The coexistence of crop and weed layers forms two-layered vegetation canopies in tall crops such as sugarcane and maize. Although radiative transfer models can simulate the weed layer’s influence on canopy reflectance and LAI inversion, few experimental investigations use in situ measurement data to verify these effects. Here, we propose a practical background modification scheme in which black material with near-zero reflectance covers the weed layer and alters the background spectrum of crop canopies. We conduct an experimental investigation in a sugarcane field with different background properties (i.e., bare soil and a weed layer). Tower-based and UAV-based hyperspectral measurements examine the spectral differences in sugarcane canopies with and without the black covering. We then use LAI measurements to evaluate the weed layer’s impact on LAI inversion from UAV-based hyperspectral data through a hybrid inversion method. We find that the weed layer significantly affects the canopy reflectance spectrum, changing it by 13.58% and 42.53% in the near-infrared region for tower-based and UAV-based measurements, respectively. Furthermore, the weed layer substantially interferes with LAI inversion of sugarcane canopies, causing significant overestimation. Estimated LAIs of sugarcane canopies with a soil background generally align well with measured values (root mean square error (RMSE) = 0.69 m2/m2), whereas those with a weed background are considerably overestimated (RMSE = 2.07 m2/m2). We suggest that this practical background modification scheme quantifies the weed layer’s influence on crop canopy reflectance from a measurement perspective and that the weed layer should be considered during the inversion of crop LAI. Full article
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20 pages, 39846 KiB  
Article
MTCDNet: Multimodal Feature Fusion-Based Tree Crown Detection Network Using UAV-Acquired Optical Imagery and LiDAR Data
by Heng Zhang, Can Yang and Xijian Fan
Remote Sens. 2025, 17(12), 1996; https://doi.org/10.3390/rs17121996 - 9 Jun 2025
Cited by 1 | Viewed by 408
Abstract
Accurate detection of individual tree crowns is a critical prerequisite for precisely extracting forest structural parameters, which is vital for forestry resources monitoring. While unmanned aerial vehicle (UAV)-acquired RGB imagery, combined with deep learning-based networks, has demonstrated considerable potential, existing methods often rely [...] Read more.
Accurate detection of individual tree crowns is a critical prerequisite for precisely extracting forest structural parameters, which is vital for forestry resources monitoring. While unmanned aerial vehicle (UAV)-acquired RGB imagery, combined with deep learning-based networks, has demonstrated considerable potential, existing methods often rely exclusively on RGB data, rendering them susceptible to shadows caused by varying illumination and suboptimal performance in dense forest stands. In this paper, we propose integrating LiDAR-derived Canopy Height Model (CHM) with RGB imagery as complementary cues, shifting the paradigm of tree crown detection from unimodal to multimodal. To fully leverage the complementary properties of RGB and CHM, we present a novel Multimodal learning-based Tree Crown Detection Network (MTCDNet). Specifically, a transformer-based multimodal feature fusion strategy is proposed to adaptively learn correlations among multilevel features from diverse modalities, which enhances the model’s ability to represent tree crown structures by leveraging complementary information. In addition, a learnable positional encoding scheme is introduced to facilitate the fused features in capturing the complex, densely distributed tree crown structures by explicitly incorporating spatial information. A hybrid loss function is further designed to enhance the model’s capability in handling occluded crowns and crowns of varying sizes. Experiments conducted on two challenging datasets with diverse stand structures demonstrate that MTCDNet significantly outperforms existing state-of-the-art single-modality methods, achieving AP50 scores of 93.12% and 94.58%, respectively. Ablation studies further confirm the superior performance of the proposed fusion network compared to simple fusion strategies. This research indicates that effectively integrating RGB and CHM data offers a robust solution for enhancing individual tree crown detection. Full article
(This article belongs to the Special Issue Digital Modeling for Sustainable Forest Management)
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18 pages, 11692 KiB  
Article
Water Balance in an Atlantic Forest Remnant: Focus on Representative Tree Species
by Adérito C. Cau, José A. Junqueira Junior, Alejandra B. Vega, Severino J. Macôo, André F. Rodrigues, Marcela C. N. S. Terra, Li Guo and Carlos R. Mello
Forests 2025, 16(5), 812; https://doi.org/10.3390/f16050812 - 13 May 2025
Viewed by 406
Abstract
The Atlantic Forest has undergone deforestation and prolonged droughts, affecting ecosystem services. This study assesses the water balance using hydrological observations from representative tree species within a Montane Semideciduous Seasonal Forest (MF) remnant. Gross precipitation (GP), canopy interception (CI), and effective precipitation (EP [...] Read more.
The Atlantic Forest has undergone deforestation and prolonged droughts, affecting ecosystem services. This study assesses the water balance using hydrological observations from representative tree species within a Montane Semideciduous Seasonal Forest (MF) remnant. Gross precipitation (GP), canopy interception (CI), and effective precipitation (EP = Throughfall + Stemflow) were recorded daily, and soil moisture was measured down to 1.80 m every two days during the dry period of the 2023/2024 hydrological year. Additionally, aboveground biomass (AGB), fresh root biomass (BR), and soil hydrological properties in the soil profile were obtained to support the water balance results. The highest EP values were recorded in Miconia willdenowii, while the lowest were in Xylopia brasiliensis. Root zone water storage exhibited a declining trend, with the highest values in Miconia willdenowii. ET remained low, mainly in April, July, and September, with Miconia willdenowii and Copaifera langsdorffii showing the highest values, and AGB correlated with CI and ET. The dynamic of this ecosystem is apparent in the temporal variations (CVt) of soil moisture, influenced by EP and ET. The greatest variability was recorded in the surface layer (0–20 cm), stabilizing with depth, especially below 120 cm. The Temporal Stability Index (TSI) of soil water storage indicated greater stability in Blepharocalyx salicifolius. This study highlights the significance of soil water storage and ET in a tropical forest ecosystem, particularly under drought conditions, suggesting potential species that may be more effective in recovering degraded areas. Full article
(This article belongs to the Section Forest Hydrology)
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21 pages, 4929 KiB  
Article
Physical–Mechanical Properties of Tomato Seedlings for the Design and Optimization of Automatic Transplanters
by Gaudencio Grande, Martín Hidalgo-Reyes, Pedro Cruz and Noé Velázquez-López
AgriEngineering 2025, 7(5), 138; https://doi.org/10.3390/agriengineering7050138 - 6 May 2025
Viewed by 569
Abstract
This study was based on the hypothesis that the hybrid type and its physical–mechanical properties significantly influence the operational efficiency of transplanting systems. Understanding these properties is essential for optimizing the performance of semi-automatic and automatic transplanters. To test this hypothesis, a completely [...] Read more.
This study was based on the hypothesis that the hybrid type and its physical–mechanical properties significantly influence the operational efficiency of transplanting systems. Understanding these properties is essential for optimizing the performance of semi-automatic and automatic transplanters. To test this hypothesis, a completely randomized design was implemented to evaluate the physical–mechanical properties of tomato seedlings. A total of 1350 seedlings from three F1 hybrids—Natalie (H1), CID (H2), and Gavilán (H3)—cultivated in central Mexico, were analyzed. The statistical analyses included mean comparisons using Tukey’s test and multiple linear regression to estimate the center of mass (CM). The results indicate that H2 was notable for its total height (ht = 311.76 mm), canopy development in X, Y, and Z axes (170.24 mm, 106.84 mm, and 98.14 mm, respectively), stem diameter (ds = 3.65 mm), total weight (wt = 11.92 g), de (78.36 mm) and dp (233.40 mm) distances, and oscillation period (T = 0.88 s). H1 had the highest stem height (hs = 53.18 mm), wt = 11.76 g, and root ball (RB) moisture content (MC) (77.36%). H3 had the largest ds = 3.70 mm, as well as the highest MC in the stem (94.51%) and the remaining foliage (92.92%). Regarding mechanical properties, the average adhesion force (AF) was 4.606 N (H1), 7.470 N (H2), and 3.815 N (H3). The average root ball punching force (RBPF) was 0.36, 0.48, and 0.25 N, respectively. The lowest static friction coefficient (SFC) on a galvanized steel sheet was 0.936. The drop test (DT) revealed an average residual substrate mass of 0.148 g at a height of 500 mm. It can be concluded that the interaction between hybrid type, transplanting age, and MC plays a critical role in the efficient design of semi-automatic and automatic transplanting equipment. This interaction enables process optimization, ensures operational quality, reduces seedling damage, and ultimately enhances and increases the long-term profitability and sustainability of the equipment. Full article
(This article belongs to the Section Agricultural Mechanization and Machinery)
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13 pages, 3963 KiB  
Article
Marked Spatial Variability in Acidity Characteristics of Purple Soil at Field Scale Induced by Citrus Plantation
by Jiayi Luo, Jingkun Zhao, Jia Zhou and Zhongyi Li
Agronomy 2025, 15(5), 1022; https://doi.org/10.3390/agronomy15051022 - 24 Apr 2025
Cited by 1 | Viewed by 381
Abstract
Purple soil, predominantly found in the Sichuan Basin of China with a favorable climate, is renowned for its fertility, making it an ideal soil for citrus cultivation. To investigate the effect of citrus plantation on the acidification characteristics of purple soil, we selected [...] Read more.
Purple soil, predominantly found in the Sichuan Basin of China with a favorable climate, is renowned for its fertility, making it an ideal soil for citrus cultivation. To investigate the effect of citrus plantation on the acidification characteristics of purple soil, we selected one field where citrus trees coexist with varying ages of 3, 10, and 50 years. The soil is a neutral purple soil developed from Jurassic Shaximiao Formation mudstone. A total of 138 soil samples were collected at different depths (0–20, 20–40, and 40–60 cm) beneath the canopies of these citrus trees for physicochemical property analysis. Our results indicate that citrus cultivation caused significant spatial variability in the purple soil acidity within the same field. The pH values of these soils varied from 3.97 to 7.90. The degree of soil acidification under the citrus canopies adheres to the following order: 10-year-old > 50-year-old > 3-year-old citrus trees. Soil pH values were negatively correlated with the contents of N, P, and K available in the soil, particularly exhibiting a significantly negative correlation with these soil fertility indicators under the canopy of the 10-year-old citrus at p < 0.01, suggesting that the intensive fertilizer application typical in citrus plantations accelerated soil acidification. Additionally, soil acidification was associated with an increase in the exchangeable Al3⁺ (from 0 to 7.03 cmol kg−1) and a decrease in the exchangeable Ca2⁺ (from 25.07 to 6.48 cmol kg−1), exchangeable Mg2⁺ (from1.53 to 0.62 cmol kg−1), base saturation (from 100% to 53.4%), and effective cation exchange capacity (from 24.3 to 13.1 cmol kg−1).The acidification of the purple soil enhanced the extractability of metal elements, increasing the bioavailability of essential plant nutrients, such as Fe, Mn, Cu, Zn, and Ni, as well as enhancing the mobility of harmful heavy metals like Pb and Cd. In conclusion, unlike the widespread acidification observed in Oxisols or Ultisols at the field scale, citrus cultivation caused varying degrees of acidification within purple soil at this scale. This variability in soil acidification at the field scale of purple soil can lead to a series of soil degradation problems and should be given due attention in the management of citrus and similar high-economic-value fruit trees. Full article
(This article belongs to the Section Innovative Cropping Systems)
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15 pages, 3049 KiB  
Article
Transcriptome Profiling Revealed Light-Mediated Gene Expression Patterns of Plants in Forest Vertical Structures
by Qiming Mei, Yi Zheng, Jiayi Feng, Zhengfeng Wang, Honglin Cao and Juyu Lian
Biology 2025, 14(4), 434; https://doi.org/10.3390/biology14040434 - 17 Apr 2025
Viewed by 586
Abstract
Light is a critical environmental factor that shapes forest communities. The canopy trees intercept the light, thus understory plants become shaded. Shade leads to the attenuation of light intensity and a shift in the spectrum through the forest vertical structure. The capacity of [...] Read more.
Light is a critical environmental factor that shapes forest communities. The canopy trees intercept the light, thus understory plants become shaded. Shade leads to the attenuation of light intensity and a shift in the spectrum through the forest vertical structure. The capacity of forest trees to survive and grow under conditions of light heterogeneity is closely related to the intrinsic property of these species. Therefore, identifying how plants interact with light-regime variability is an important research objective of community ecology. In this study, we investigated the light-mediated gene expression patterns in forest vertical structures utilizing transcriptome profiling. The expression levels of 20 annotated genes closely related to photosynthesis, light receptors, and photoprotection were used as traits to estimate how variable light environments influence the plants in forest vertical structures. In summary, the shade-tolerant species were characterized by higher levels of photoreceptor (phot1/2 and phyA/B), photorespiration (pglp1/2), and photoprotection genes (Lhca5, Lhca7, and PsbS and photolyases), but with a lower abundance of photosynthetic light-harvesting genes (Lhca1/2 and Lhcb1/2). Also, the expression of light-harvesting and photoprotection genes were generally up-regulated by intense light, while the expression of photoreceptor genes was up-regulated by shade. This research highlights how differential plant responses to light shape the vertical structure of plant communities in a subtropical forest. Full article
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26 pages, 2420 KiB  
Article
Runoff and Evapotranspiration–Precipitation Ratios as Indicators of Water Regulation Ecosystem Services in Urban Forests
by Urša Vilhar
Land 2025, 14(4), 809; https://doi.org/10.3390/land14040809 - 9 Apr 2025
Viewed by 920
Abstract
As a form of green infrastructure, urban forests play a key role in the provision of hydrological ecosystem services (ESs) in cities. Understanding how urban forest structure and soil properties influence water regulation ESs is crucial for managing and planning green infrastructure in [...] Read more.
As a form of green infrastructure, urban forests play a key role in the provision of hydrological ecosystem services (ESs) in cities. Understanding how urban forest structure and soil properties influence water regulation ESs is crucial for managing and planning green infrastructure in cities. We analysed two indicators—the runoff to precipitation (Q/P) and the evapotranspiration to precipitation (ETP/P) ratios—for five different urban forests. We used the hydrological model Brook90 over 16 years to simulate runoff, evapotranspiration, canopy interception, transpiration and soil evaporation. The results showed that mixed forests have the highest water retention capacity, with the lowest Q/P (0.41) and the highest ETP/P (0.59). In contrast, riparian deciduous forests had the lowest water retention capacity, with the highest Q/P (0.75) and the lowest ETP/P (0.25). Both indicators showed similar annual and seasonal results. However, Q/P showed strong inter-annual variation and a strong correlation with precipitation, while ETP/P remained consistent despite precipitation fluctuations in the observed years. In conclusion, the ETP/P ratio is better suited to assess the water regulation ES of urban forests. Full article
(This article belongs to the Special Issue Urban Ecosystem Services: 6th Edition)
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18 pages, 3001 KiB  
Review
Adaptive Viticulture Strategies to Enhance Resilience and Grape Quality in Cold Climate Regions in Response to Climate Warming
by Gastón Gutiérrez-Gamboa and Ana Mucalo
Horticulturae 2025, 11(4), 394; https://doi.org/10.3390/horticulturae11040394 - 8 Apr 2025
Viewed by 1397
Abstract
Cold climate viticulture is challenged by climatic variability, including increased frost risk, shorter growing seasons, and unpredictable weather events that impact vine productivity and grape quality. Global warming is altering traditional viticulture zones, prompting the exploration of new regions for grape cultivation, the [...] Read more.
Cold climate viticulture is challenged by climatic variability, including increased frost risk, shorter growing seasons, and unpredictable weather events that impact vine productivity and grape quality. Global warming is altering traditional viticulture zones, prompting the exploration of new regions for grape cultivation, the selection of climate-resilient cultivars, and the implementation of adaptive practices. This review synthesizes recent advances in adaptive viticulture practices and plant growth regulator applications, highlighting novel molecular and physiological insights on cold stress resilience and berry quality. Key strategies include delayed winter pruning to mitigate frost damage, osmoprotectant application to improve freeze tolerance, and canopy management techniques (cluster thinning and defoliation) to enhance berry ripening and wine composition. Their effectiveness depends on vineyard microclimate, soil properties and variety-specific physiological response. Cover cropping is examined for its role in vine vigor regulation, improving soil microbial diversity, and water retention, though its effectiveness depends on soil type, participation patterns, and vineyard management practices. Recent transcriptomic and metabolomic studies have provided new regulatory mechanisms in cold stress adaptation, highlighting the regulatory roles of abscisic acid, brassinosteroids, ethylene, and salicylic acid in dormancy induction, oxidative stress response, and osmotic regulation. Reflective mulch technologies are currently examined for their ability to enhance light interception, modulating secondary metabolite accumulation, improving technological maturity (soluble solids, pH, and titratable acidity) and enhancing phenolic compounds content. The effectiveness of these strategies remains highly site-specific, influenced by variety selection and pruning methods particularly due to their differences on sugar accumulation and berry weight. Future research should prioritize long-term vineyard trials to refine these adaptive strategies, integrate genetic and transcriptomic insights into breeding programs to improve cold hardiness, and develop precision viticulture tools tailored to cold climate vineyard management. Full article
(This article belongs to the Section Viticulture)
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17 pages, 3405 KiB  
Article
Application of a Novel Formulation of 1-Aminocyclopropane-1-carboxylic Acid (ACC) to Increase the Anthocyanins Concentration in Table Grape Berries
by Aline Cristina de Aguiar, Danielle Mieko Sakai, Bianca Liriel Martins Barbosa, Stefanie do Prado da Silva, Fábio Yamashita and Sergio Ruffo Roberto
Plants 2025, 14(7), 1058; https://doi.org/10.3390/plants14071058 - 29 Mar 2025
Viewed by 438
Abstract
The objective of this work was to assess different concentrations of a novel formulation of 1-aminocyclopropane-1-carboxylic acid (ACC) on anthocyanin accumulation and color development, as well as on the physicochemical characteristics of the ‘Benitaka’ table grape grown in a subtropical region in two [...] Read more.
The objective of this work was to assess different concentrations of a novel formulation of 1-aminocyclopropane-1-carboxylic acid (ACC) on anthocyanin accumulation and color development, as well as on the physicochemical characteristics of the ‘Benitaka’ table grape grown in a subtropical region in two application forms. The trial was conducted on a commercial property located in a subtropical area in Brazil in 2022. Treatments included different concentrations of a new formulation containing 400 g kg−1 of ACC, ranging from 0 to 125 g 100 L−1, as well as a standard concentration of a formulation containing 100 g L−1 of abscisic acid (S-ABA): 3.2 L ha−1. The exogenous application of ACC was performed at the beginning of berry ripening (véraison), while that of S-ABA was performed twice: the first, at véraison, and the second, 7 days later. The concentration of total anthocyanins, berry color index, physicochemical characteristics, and sensory–visual analysis of color coverage of the bunches were evaluated weekly, while berry firmness was appraised at harvest. A single exogenous application of ACC or two applications of S-ABA resulted in daily increment rates that provided a high accumulation of total anthocyanins, as well as greater berry color development, regardless of the application method, directed to the canopy of the vines or only to the bunches. As a result, the new formulation of ACC at concentrations of 75 g to 100 g 100 L−1 is a novel tool to stimulate the anthocyanins accumulation and berry color development in ‘Benitaka’ table grapes grown in subtropical regions without negative impact on bunches or vines. Full article
(This article belongs to the Special Issue Research on Nutritional and Bioactive Compounds from Edible Fruits)
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