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Keywords = canopy hyperspectral reflectance

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33 pages, 5528 KB  
Article
Multisensor Monitoring of Soil–Plant–Atmosphere Interactions During Reproductive Development in Wheat
by Sandra Skendžić, Darija Lemić, Hrvoje Novak, Marko Reljić, Marko Maričević, Vinko Lešić, Ivana Pajač Živković and Monika Zovko
AgriEngineering 2026, 8(3), 119; https://doi.org/10.3390/agriengineering8030119 - 20 Mar 2026
Viewed by 653
Abstract
Assessing crop water status during the reproductive development of winter wheat is challenging because soil–plant–atmosphere interactions are strongly influenced by soil physical conditions, and measured soil water content (SWC) does not necessarily reflect plant-accessible water. This study applied an integrated, process-based multisensor approach [...] Read more.
Assessing crop water status during the reproductive development of winter wheat is challenging because soil–plant–atmosphere interactions are strongly influenced by soil physical conditions, and measured soil water content (SWC) does not necessarily reflect plant-accessible water. This study applied an integrated, process-based multisensor approach to evaluate functional crop water status and its relationship to grain yield, combining hyperspectral canopy reflectance, atmospheric observations, in situ SWC, and pedological characterization. Five winter wheat cultivars were monitored at two contrasting pedoclimatic sites in continental Croatia during the 2022/2023 growing season. Hyperspectral canopy reflectance (350–2500 nm) was measured at reproductive stages (BBCH 61–83), and seventeen vegetation indices describing canopy water status, structure, pigments, and senescence were derived. Principal component analysis (PCA) identified location as the dominant source of spectral variability, while cultivar effects were secondary. Although atmospheric conditions were broadly comparable, the sites differed markedly in soil physical properties, resulting in contrasting soil water–air regimes. Despite consistently higher volumetric SWC at one site, hyperspectral indicators revealed lower canopy water status, reduced canopy structure, earlier senescence, and lower grain yield across all cultivars. Water-sensitive indices exploiting near-infrared (700–1300 nm) and shortwave infrared (1300–2400 nm) bands (NDWI, NDMI, NMDI, MSI) consistently indicated greater physiological stress. Conversely, the site with lower SWC but more favorable soil physical conditions exhibited higher values of water- and structure-related indices and achieved higher grain yield, with a mean increase of 669 kg ha−1. The results demonstrate that hyperspectral canopy reflectance captures yield-relevant water stress that cannot be inferred from soil moisture alone, highlighting the importance of multisensor integration for interpreting soil–plant–atmosphere interactions under heterogeneous soil conditions. Full article
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24 pages, 6483 KB  
Article
Integrating Plant Height into Hyperspectral Inversion Models for Estimating Chlorophyll and Total Nitrogen in Rice Canopies
by Jing He, Yangyang Song, Dong Xie and Gang Liu
Agriculture 2026, 16(6), 656; https://doi.org/10.3390/agriculture16060656 - 13 Mar 2026
Viewed by 527
Abstract
Rice undergoes rapid growth and exhibits a high demand for nutrients during the tillering and booting stages. SPAD readings, which reflect relative leaf chlorophyll status, and leaf nitrogen content (LNC) are key indicators of plant nutritional status, directly influencing photosynthetic efficiency and biomass [...] Read more.
Rice undergoes rapid growth and exhibits a high demand for nutrients during the tillering and booting stages. SPAD readings, which reflect relative leaf chlorophyll status, and leaf nitrogen content (LNC) are key indicators of plant nutritional status, directly influencing photosynthetic efficiency and biomass accumulation, while plant height (PH) reflects canopy structure and nutrient availability. Establishing quantitative relationships among these traits at key growth stages is essential for stage-specific precision rice management. In this study, Unmanned Aerial Vehicle (UAV) hyperspectral imagery and ground-truth measurements of SPAD, LNC, and PH were collected from rice fields in Qingbaijiang District, Chengdu, China. Twelve vegetation indices (VIs) were calculated, and three machine learning algorithms—partial least squares regression (PLSR), support vector regression (SVR), and random forest regression (RFR)—were employed to develop stage-specific retrieval models. A stage-specific modeling framework integrating PH with hyperspectral data was developed to statistically enhance estimation accuracy at the tillering and booting stages. The optimal models for SPAD readings and LNC achieved R2 values of 0.916 and 0.936, respectively. The results indicate that integrating canopy structural information with hyperspectral features can improve the estimation accuracy of SPAD-related chlorophyll indicators and nitrogen status in rice. Under the controlled field conditions of this study, the proposed framework provides a plot-scale proof-of-concept demonstration for UAV-based stage-specific nitrogen monitoring. Full article
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25 pages, 14850 KB  
Article
Remote Sensing of Rice Canopy Nitrogen Content Based on Unmanned Aerial Vehicle Multi-Angle Polarized Hyperspectral Data
by Chenyi Xu, Shuang Xiang, Nan Wang, Fenghua Yu and Zhonghui Guo
Remote Sens. 2026, 18(6), 876; https://doi.org/10.3390/rs18060876 - 12 Mar 2026
Viewed by 444
Abstract
Nitrogen is one of the essential nutrient elements that affect rice growth, yield, and quality formation. Accurate and timely estimation of rice nitrogen status is fundamental for precision fertilization in agricultural fields. Hyperspectral remote sensing technology provides a promising approach for rapid and [...] Read more.
Nitrogen is one of the essential nutrient elements that affect rice growth, yield, and quality formation. Accurate and timely estimation of rice nitrogen status is fundamental for precision fertilization in agricultural fields. Hyperspectral remote sensing technology provides a promising approach for rapid and accurate acquisition of nitrogen status of rice in the field. However, traditional single-angle hyperspectral observations are easily disturbed by factors such as canopy structure, light direction, and background reflection, limiting their inversion accuracy and stability. This study is based on multi-angle polarimetric hyperspectral data obtained from an unmanned aerial vehicle platform. It extracts features from multi-angle polarimetric spectra based on three algorithms: successive projections algorithm (SPA), competitive adaptive reweighted sampling, and relevant features. The input weight and hidden layer bias of the extreme learning machine (ELM) model were optimized by the whale optimization algorithm (WOA) and caterpillar fungus optimization algorithm (CFO), taking the sensitive band of optimal viewing angle as input. Finally, an inversion model of rice canopy nitrogen content (CNC) based on multi-angle polarization hyperspectral data was established. The results demonstrate that the inversion results of the combination of SPA-(30°) + SPA-(45°) observation angles and feature selection methods are optimal, and multi-angle fusion significantly improves the model’s ability to characterize CNC, with higher stability and accuracy than single-angle modeling. The R2 of CFO-ELM on the training set and test set reach 0.8553 and 0.8274, respectively, which is significantly better than the original ELM and WOA-ELM, becoming the optimal CNC inversion model in this study. The rice CNC inversion model based on multi-angle polarimetric hyperspectral data constructed in this study provides a specific reference for the rapid detection of rice CNC. Full article
(This article belongs to the Section Remote Sensing in Agriculture and Vegetation)
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23 pages, 2586 KB  
Article
Explainable AI-Based Hyperspectral Classification Reveals Differences in Spectral Response over Phenological Stages
by Rameez Ahsen, Pierpaolo Di Bitonto, Pierfrancesco Novielli, Michele Magarelli, Donato Romano, Martina Di Venosa, Anna Maria Stellacci, Nicola Amoroso, Alfonso Monaco, Bruno Basso, Roberto Bellotti and Sabina Tangaro
Biology 2026, 15(6), 454; https://doi.org/10.3390/biology15060454 - 11 Mar 2026
Viewed by 536
Abstract
Optimizing nitrogen (N) fertilization is essential for sustaining durum wheat yield and grain quality while reducing the environmental impacts associated with N over-application. Hyperspectral sensing provides a rapid and non-destructive approach for monitoring crop N status. However, high-dimensional data, phenology-dependent spectral responses, and [...] Read more.
Optimizing nitrogen (N) fertilization is essential for sustaining durum wheat yield and grain quality while reducing the environmental impacts associated with N over-application. Hyperspectral sensing provides a rapid and non-destructive approach for monitoring crop N status. However, high-dimensional data, phenology-dependent spectral responses, and spatial autocorrelation in field measurements limit robust nitrogen classification and interpretation. This study evaluated hyperspectral-based nitrogen status classification in durum wheat under Mediterranean field conditions and identified key spectral regions using explainable artificial intelligence. A field experiment was conducted in Southern Italy using ten N fertilization rates (0–180 kg N ha−1). Canopy reflectance was acquired at the booting and heading stages from georeferenced sampling locations. Three nitrogen stratification strategies (binary Low–High, Extreme, and three-level) were evaluated using Random Forest, SVM-RBF, and XGBoost classifiers. Model performance was assessed using spatially independent Leave-One-Plot-Out cross-validation at both the sample and plot levels, with plot-level predictions derived through majority voting. Classification robustness was strongly influenced by the stratification strategy and phenological stage. The binary Low–High stratification achieved the highest sample-level accuracy, with a maximum of 0.78 at booting (SVM-RBF) and 0.75 at heading (SVM-RBF), whereas the Extreme stratification produced intermediate performance, with maximum accuracies of 0.73 at booting (SVM-RBF) and 0.63 at heading (XGBoost). Plot-level aggregation improved performance, reaching up to 0.90 at booting and 1.00 at heading. SHAP analysis highlighted red, red-edge, and near-infrared wavelengths as the dominant contributors, with increased reliance on longer wavelengths at the heading. Overall, explainable machine learning provides a robust framework for hyperspectral nitrogen monitoring in durum wheat. Full article
(This article belongs to the Special Issue Adaptation of Living Species to Environmental Stress (2nd Edition))
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25 pages, 5640 KB  
Article
Estimation of Winter Wheat SPAD Values by Integrating Spectral Feature Optimization and Machine Learning Algorithms
by Yufei Wang, Xuebing Wang, Jiang Sun, Zeyang Wen, Haoyong Wu, Lujie Xiao, Meichen Feng, Yu Zhao and Xianjie Gao
Agronomy 2026, 16(4), 489; https://doi.org/10.3390/agronomy16040489 - 22 Feb 2026
Viewed by 760
Abstract
The chlorophyll content of plant leaves measured by the soil plant analysis development (SPAD) is an important indicator for measuring crop growth status and irrigation effect. The rapid, non-destructive and efficient estimation of crop SPAD values is of great significance to the field [...] Read more.
The chlorophyll content of plant leaves measured by the soil plant analysis development (SPAD) is an important indicator for measuring crop growth status and irrigation effect. The rapid, non-destructive and efficient estimation of crop SPAD values is of great significance to the field management of crops. In this study, the canopy hyperspectral reflectance and SPAD values of winter wheat were obtained, and the spectral curve was changed through four spectral processing methods, including first-order differential (FD), second-order differential (SD), multivariate scattering correction (MSC), and Savitzky–Golay smoothing (SG) to improve the correlation between canopy spectral reflectance and SPAD. Furthermore, to investigate and evaluate the performance of various vegetation indices (VIs) in estimating SPAD values for winter wheat, existing published indices were optimized using random band combinations derived from multiple canopy spectral transformations. The optimized vegetation index was used as the input variable of the model, and six machine learning algorithms, including random forest (RF), long short-term memory network (LSTM), multilayer perceptron (MLP), deep recurrent neural network (Deep-RNN), gated recurrent unit (GRU), and convolutional neural network (CNN), were used to construct the winter wheat SPAD values estimation model, and the model was verified. The experimental results demonstrate that, when utilizing an equivalent number of optimized vegetation indices as input, the GRU-based model achieves higher estimation accuracy compared to other models. Specifically, the coefficient of determination (R2) is improved by 0.12 compared to the RF model, by 0.03 compared to the LSTM model, by 0.12 compared to the MLP model, by 0.02 compared to the Deep-RNN model, and by 0.02 compared to the CNN model. At the same time, the GRU model also has a lower root mean square error (RMSE) and relative error (RE) of 7.37 and 24.90%, respectively. This study provides valuable hyperspectral remote sensing technology support for the implementation of winter wheat SPAD values estimation in the field. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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28 pages, 25207 KB  
Article
Identification of Plastic Mulch in Cotton Fields Using UAV-Based Hyperspectral Data and Deep Learning Semantic Segmentation
by Qingyao Zhao, Shenglin Li, Fukui Gao, Huifeng Ning, Dongke Dai, Pengyuan Zhu, Nanfang Li, Yinping Song, Caixia Li and Hao Liu
Agronomy 2026, 16(4), 458; https://doi.org/10.3390/agronomy16040458 - 15 Feb 2026
Viewed by 671
Abstract
Plastic mulching is widely used in arid and semi-arid cotton systems to improve soil hydrothermal conditions and water–nutrient use efficiency. However, residual mulch and its potential contribution to microplastic inputs pose growing environmental and soil-quality risks, highlighting the need for high-resolution and automated [...] Read more.
Plastic mulching is widely used in arid and semi-arid cotton systems to improve soil hydrothermal conditions and water–nutrient use efficiency. However, residual mulch and its potential contribution to microplastic inputs pose growing environmental and soil-quality risks, highlighting the need for high-resolution and automated approaches to support plastic waste management, targeted retrieval, and precision field operations. Taking a mulched cotton field in Alar, Xinjiang, as the study area, this study proposes a novel plastic mulch extraction method that integrates Unmanned Aerial Vehicle (UAV)-based hyperspectral imagery with deep learning semantic segmentation. The Jeffries–Matusita (JM) distance was employed to select highly separable optimal bands and their combinations for discriminating plastic mulch, bare soil, and cotton canopy, which were then used to drive UNet, DeepLabV3+, and PSPNet models for plastic mulch mapping. The results indicate that the PSPNet model driven by the 402 nm single-band reflectance, Normalized Difference Index (NDI) (861 nm, 410 nm), and NDI (757 nm, 676 nm) achieved the best performance for plastic mulch identification (Intersection over Union (IoU) = 80.28%), significantly outperforming the RGB-based model (IoU = 76.51%). This study enables accurate, spatially explicit assessments of residual mulch, providing actionable evidence for plastic waste monitoring and management, while supporting sustainable agriculture and precision farmland management. Full article
(This article belongs to the Special Issue Water–Salt in Farmland: Dynamics, Regulation and Equilibrium)
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21 pages, 11722 KB  
Article
Simultaneous Hyperspectral and Radar Satellite Measurements of Soil Moisture for Hydrogeological Risk Monitoring
by Kalliopi Karadima, Andrea Massi, Alessandro Patacchini, Federica Verde, Claudia Masciulli, Carlo Esposito, Paolo Mazzanti, Valeria Giliberti and Michele Ortolani
Remote Sens. 2026, 18(3), 393; https://doi.org/10.3390/rs18030393 - 24 Jan 2026
Cited by 1 | Viewed by 890
Abstract
Emerging landslides and severe floods highlight the urgent need to analyse and support predictive models and early warning systems. Soil moisture is a crucial parameter and it can now be determined from space with a resolution of a few tens of meters, potentially [...] Read more.
Emerging landslides and severe floods highlight the urgent need to analyse and support predictive models and early warning systems. Soil moisture is a crucial parameter and it can now be determined from space with a resolution of a few tens of meters, potentially leading to the continuous global monitoring of landslide risk. We address this issue by determining the volumetric water content (VWC) of a testbed in Southern Italy (bare soil with significant flood and landslide hazard) through the comparison of two different satellite observations on the same day. In the first observation (Sentinel-1 mission of the European Space Agency, C-band Synthetic Aperture Radar (SAR)), the back-scattered radar signal is used to determine the VWC from the dielectric constant in the microwave range, using a time-series approach to calibrate the algorithm. In the second observation (hyperspectral PRISMA mission of the Italian Space Agency), the short-wave infrared (SWIR) reflectance spectra are used to calculate the VWC from the spectral weight of a vibrational absorption line of liquid water (wavelengths 1800–1950 nm). As the main result, we obtained a Pearson’s correlation coefficient of 0.4 between the VWC values measured with the two techniques and a separate ground-truth confirmation of absolute VWC values in the range of 0.10–0.30 within ±0.05. This overlap validates that both SAR and hyperspectral data can be well calibrated and mapped with 30 m ground resolution, given the absence of artifacts or anomalies in this particular testbed (e.g., vegetation canopy or cloud presence). If hyperspectral data in the SWIR range become more broadly available in the future, our systematic procedure to synchronise these two technologies in both space and time can be further adapted to cross-validate the global high-resolution soil moisture dataset. Ultimately, multi-mission data integration could lead to quasi-real-time hydrogeological risk monitoring from space. Full article
(This article belongs to the Special Issue Remote Sensing in Geomatics (Second Edition))
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26 pages, 5020 KB  
Article
Utilising the Potential of a Robust Three-Band Hyperspectral Vegetation Index for Monitoring Plant Moisture Content in a Summer Maize-Winter Wheat Crop Rotation Farming System
by James E. Kanneh, Caixia Li, Yanchuan Ma, Shenglin Li, Madjebi Collela BE, Zuji Wang, Daokuan Zhong, Zhiguo Han, Hao Li and Jinglei Wang
Remote Sens. 2026, 18(2), 271; https://doi.org/10.3390/rs18020271 - 14 Jan 2026
Cited by 2 | Viewed by 900
Abstract
Water is vital for producing summer maize (SM) and winter wheat (WW); therefore, its proper management is crucial for sustainable farming. This study aimed to develop new tri-band spectral vegetation indices that enhance the accuracy of monitoring plant moisture content (PMC) [...] Read more.
Water is vital for producing summer maize (SM) and winter wheat (WW); therefore, its proper management is crucial for sustainable farming. This study aimed to develop new tri-band spectral vegetation indices that enhance the accuracy of monitoring plant moisture content (PMC) in SM and WW. We conducted irrigation treatments, including W0, W1, W2, W3, and W4, in SM–WW rotations to address this issue. Canopy reflectance was measured with a field spectroradiometer. Tri-band hyperspectral vegetation indices were constructed: Normalised Water Stress Index (NWSI), Normalised Difference Index (NDI), and Exponential Water Stress Index (EWSI), for assessing the PMC of SM and WW. Results indicate that NWSI outperformed other indices. In the maize trials, the correlation reached R = −0.8369, while in wheat, it reached R = −0.9313, surpassing traditional indices. Four mainstream machine learning models (Random Forest, Partial Least Squares Regression, Support Vector Machine, and Artificial Neural Network) were employed for modelling. NWSI-PLSR exhibited the best index-type performance with an R2 of 0.7878. When the new indices were combined with traditional indices as input data, the NWSI-Published indices-SVM model achieved superior performance with an R2 of 0.8203, outperforming other models. The RF model produced the most consistent performance and achieved the highest average R2 across all input types. The NDI-Published indices models also outperformed those of the published indices alone. This indicates that these new indices improve the accuracy of moisture content monitoring in SM and WW fields. It provides a technical basis and support for precision irrigation, holding significant potential for application. Full article
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23 pages, 8140 KB  
Article
Comparative Assessment of Hyperspectral and Multispectral Vegetation Indices for Estimating Fire Severity in Mediterranean Ecosystems
by José Alberto Cipra-Rodriguez, José Manuel Fernández-Guisuraga and Carmen Quintano
Remote Sens. 2026, 18(2), 244; https://doi.org/10.3390/rs18020244 - 12 Jan 2026
Viewed by 726
Abstract
Assessing post-fire disturbance in Mediterranean ecosystems is essential for quantifying ecological impacts and guiding restoration strategies. This study evaluates fire severity following an extreme wildfire event (~28,000 ha) in northwestern Spain using vegetation indices (VIs) derived from PRISMA hyperspectral imagery, validated against field-based [...] Read more.
Assessing post-fire disturbance in Mediterranean ecosystems is essential for quantifying ecological impacts and guiding restoration strategies. This study evaluates fire severity following an extreme wildfire event (~28,000 ha) in northwestern Spain using vegetation indices (VIs) derived from PRISMA hyperspectral imagery, validated against field-based Composite Burn Index (CBI) measurements at the vegetation, soil, and site levels across three vegetation formations (coniferous forests, broadleaf forests, and shrublands). Hyperspectral VIs were benchmarked against multispectral VIs derived from Sentinel-2. Hyperspectral VIs yielded stronger correlations with CBI values than multispectral VIs. Vegetation-level CBI showed the highest correlations, reflecting the sensitivity of most VIs to canopy structural and compositional changes. Indices incorporating red-edge, near-infrared (NIR), and shortwave infrared (SWIR) bands demonstrated the greatest explanatory power. Among hyperspectral indices, DVIRED, EVI, and especially CAI performed best. For multispectral data, NDRE, CIREDGE, ENDVI, and GNDVI were the most effective. These findings highlight the strong potential of hyperspectral remote sensing for accurate, scalable post-fire severity assessment in heterogeneous Mediterranean ecosystems. Full article
(This article belongs to the Section Forest Remote Sensing)
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24 pages, 5841 KB  
Article
Probing Early and Long-Term Drought Responses in Kauri Using Canopy Hyperspectral Imaging
by Mark Jayson B. Felix, Russell Main, Michael S. Watt and Taoho Patuawa
Remote Sens. 2025, 17(23), 3914; https://doi.org/10.3390/rs17233914 - 3 Dec 2025
Viewed by 1166
Abstract
Global increases in drought frequency and severity pose growing risks to forest resilience, particularly for long-lived endemic tree species such as kauri (Agathis australis). Building on prior leaf-level work, this study assessed the utility of multitemporal canopy-scale hyperspectral imaging to characterise [...] Read more.
Global increases in drought frequency and severity pose growing risks to forest resilience, particularly for long-lived endemic tree species such as kauri (Agathis australis). Building on prior leaf-level work, this study assessed the utility of multitemporal canopy-scale hyperspectral imaging to characterise water stress in both controlled nursery and field conditions. Two complementary experiments were undertaken: (i) a 10-week controlled-environment experiment comparing drought and control groups, and (ii) a field-based assessment of juvenile kauri trees across multiple time points with contrasting soil volumetric water content. In the controlled-environment experiment, drought-treated seedlings exhibited delayed physiological responses, with reductions in stomatal conductance and assimilation emerging only after three weeks. In contrast, time-series analysis of narrow band hyperspectral indices (NBHIs) revealed detectable stress signatures within one week after drought initiation, with early sensitivity driven by structural and pigment-related indices. As stress progressed, pigment-specific indices became the dominant predictors. These findings were consistent with the field-based experiment. Variation in leaf equivalent water thickness (EWT) was strongly explained by pigment-sensitive indices, including Pigment Specific Simple Ratio Carotenoid (PSSRc) and Carotenoid Reflectance indices (CRI700 and CRI550), which together accounted for ca. 87% of the variance. Structural indices such as the Normalised Difference Vegetation Index (NDVI) also ranked among the top 20 predictors, but had comparatively lower explanatory power (<75%). Overall, the two experiments show that canopy-based hyperspectral imaging provides early, sensitive, and consistent detection of water stress in kauri. The findings highlight a scalable approach for monitoring drought impacts on kauri and offer a foundation for developing operational forest health tools under increasing climate pressure. Full article
(This article belongs to the Collection Feature Papers for Section Environmental Remote Sensing)
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20 pages, 8079 KB  
Article
How Do Spectral Scales and Machine Learning Affect SPAD Monitoring at Different Growth Stages of Winter Wheat?
by Xueqing Zhu, Jun Li, Yali Sheng, Weiqiang Wang, Haoran Wang, Hui Yang, Ying Nian, Jikai Liu and Xinwei Li
Agriculture 2025, 15(23), 2410; https://doi.org/10.3390/agriculture15232410 - 22 Nov 2025
Viewed by 618
Abstract
Chlorophyll serves as a crucial indicator for crop growth monitoring and reflects the health status of crops. Hyperspectral remote sensing technology, leveraging its advantages of repeated observations and high-throughput analysis, provides an effective approach for non-destructive chlorophyll monitoring. However, determining the optimal spectral [...] Read more.
Chlorophyll serves as a crucial indicator for crop growth monitoring and reflects the health status of crops. Hyperspectral remote sensing technology, leveraging its advantages of repeated observations and high-throughput analysis, provides an effective approach for non-destructive chlorophyll monitoring. However, determining the optimal spectral scale remains the primary bottleneck constraining the widespread application of hyperspectral remote sensing in crop chlorophyll estimation: excessively fine spectral scale readily introduces redundant information, leading to dramatically increased data dimensions and reduced computational efficiency; conversely, overly coarse spectral scale risks losing critical spectral features such as absorption peaks and reflection troughs, thereby compromising model accuracy. Therefore, establishing an appropriate spectral scale that effectively preserves spectral feature information while maintaining computational efficiency is crucial for enhancing the accuracy and practicality of chlorophyll remote sensing estimation. To address this, this study proposes a three-dimensional analytical framework integrating “spectral scale—machine learning algorithm—crop growth stage” to systematically solve the scale optimization problem. Ground-truth measurements and hyperspectral data from five growth stages of winter wheat in Fengyang County, Anhui Province, were collected. Spectral bands sensitive to chlorophyll were analyzed, and four modeling methods—Ridge Regression (RR), K-Nearest Neighbors (KNN), Random Forest (RF), and Support Vector Regression (SVR)—were employed to integrate data from different spectral scales with respective bandwidths of 2, 3, 5, 7, 10, 20, and 50 nanometers (nm). The results evaluated the response characteristics of raw band reflectance to chlorophyll values and its impact on machine learning-based chlorophyll estimation across different spectral scales. Results indicate: (1) Canopy spectra significantly correlated with winter wheat chlorophyll primarily reside in the red and red-edge bands; (2) For single-scale analysis, larger spectral scales (10, 20 nm) enhance monitoring accuracy compared to 1 nm high-resolution data, while medium and small scales (5, 7 nm) may degrade accuracy due to redundant noise introduction. (3) Integrating growth stages, spectral scales, and machine learning revealed optimal monitoring accuracy during the jointing and heading stages using 1–5 nm spectral scales combined with the KNN algorithm. For the booting, flowering, and grain filling stages, the highest accuracy was achieved using 20–50 nm spectral scales combined with either the KNN or RF algorithm. The results indicate that high-precision chlorophyll inversion for winter wheat does not rely on a single fixed model or scale, but rather on the dynamic adaptation of the “scale-model-growth stage” triad. The proposed systematic framework not only provides a theoretical basis for chlorophyll monitoring using multi-platform remote sensing data, but also offers methodological support for future crop-sensing sensor design and data processing strategy optimization. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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19 pages, 1792 KB  
Article
Hyperspectral Detection of Single and Combined Effects of Simulated Tree Shading and Alternaria alternata Infection on Sorghum bicolor, from Leaf to UAV-Canopy Scale
by Lorenzo Pippi, Michael Alibani, Nicola Acito, Daniele Antichi, Giovanni Caruso, Marco Fontanelli, Michele Moretti, Cristina Nali, Silvia Pampana, Elisa Pellegrini, Andrea Peruzzi, Samuele Risoli, Gabriele Sileoni, Nicola Silvestri, Lorenzo Gabriele Tramacere and Lorenzo Cotrozzi
Agronomy 2025, 15(11), 2458; https://doi.org/10.3390/agronomy15112458 - 22 Oct 2025
Cited by 1 | Viewed by 1007
Abstract
Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the [...] Read more.
Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the urgent need for advanced plant health monitoring in these systems. This study assessed the potential of hyperspectral reflectance to detect the single and combined effects of simulated tree shading and infection by the fungal pathogen Alternaria alternata on grain sorghum (Sorghum bicolor L. Moench) under rainfed field conditions. Sorghum was grown either under full light or 50% shading conditions. Half of the plots were artificially inoculated with an A. alternata spore suspension (2 × 108 CFU mL−1), while the others served as controls. Leaf and ground-canopy measurements were acquired with a full range spectroradiometer (VNIR-SWIR, 400–2,400 nm) and UAV imagery covered the VIS-NIR range (400–1,000 nm) before the onset of visible symptoms. Permutational multivariate analysis of variance of leaf and ground-canopy data revealed significant effects of shading (Sh), infection (Aa), and their interaction (p < 0.05), allowing early detection of infection two days before symptom appearance, while UAV data showed only singular significant effects. Partial least squares discriminant analysis accuracy reached 78% at the leaf level, 90% at the ground-canopy level, and 74% (Sh) and 75% (Aa) at the UAV scale. Furthermore, vegetation spectral indices derived from the spectra confirmed greater physiological stress in shaded and infected plants, consistent with disease incidence assessments. Our results establish scale-specific hyperspectral reflectance spectroscopy as a powerful, non-destructive technique for early plant health surveillance in agroforestry. This advanced optical sensing capability is poised to illuminate complex stressor interactions, marking a significant step forward for precision agroforestry management. Full article
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18 pages, 6804 KB  
Article
Three-Dimensional Spectral Index-Driven Nondestructive Quantification of Chlorophyll in Winter Wheat: Cross-Phenology Extrapolation and Independent Validation
by Zhijun Li, Wei Zhang, Zijun Tang, Youzhen Xiang and Fucang Zhang
Agronomy 2025, 15(10), 2376; https://doi.org/10.3390/agronomy15102376 - 11 Oct 2025
Cited by 1 | Viewed by 863
Abstract
As a staple cereal worldwide, winter wheat plays a pivotal role in food security. Leaf chlorophyll serves as a direct indicator of photosynthetic performance and nitrogen nutrition, making it critical for precision management and yield gains. Consequently, rapid, nondestructive, and high-accuracy remote-sensing retrievals [...] Read more.
As a staple cereal worldwide, winter wheat plays a pivotal role in food security. Leaf chlorophyll serves as a direct indicator of photosynthetic performance and nitrogen nutrition, making it critical for precision management and yield gains. Consequently, rapid, nondestructive, and high-accuracy remote-sensing retrievals are urgently needed to underpin field operations and precision fertilization. In this study, canopy hyperspectral reflectance together with destructive chlorophyll assays were systematically acquired from Yangling field trials conducted during 2018–2020. Three families of spectral indices were devised: classical empirical indices; two-dimensional optimal spectral indices (2D OSI) selected by correlation-matrix screening; and novel three-dimensional optimal spectral indices (3D OSI). The main contribution lies in devising novel 3D OSIs that combine three spectral bands and demonstrating how their fusion with classic two-band indices can improve chlorophyll quantification. Correlation analysis showed that most empirical vegetation indices were significantly associated with chlorophyll (p < 0.05), with the new double difference index (NDDI) giving the strongest relationship (R = 0.637). Within the optimal-index sets, the difference three-dimensional spectral index (DTSI; 680, 807, and 1822 nm) achieved a correlation coefficient of 0.703 (p < 0.05). Among all multi-input fusion schemes, fusing empirical indices with 3D OSI and training with RF delivered the best validation performance (R2 = 0.816, RMSE = 0.307 mg g−1, MRE = 11.472%), and external data further corroborated its feasibility. Altogether, integrating 3D spectral indices with classical vegetation indices and deploying RF enabled accurate, nondestructive estimation of winter wheat chlorophyll, offering a new hyperspectral pathway for monitoring crop physiological status and advancing precision agricultural management and fertilization, can guide in-season fertilization to optimize nitrogen use, thereby advancing precision agriculture. Full article
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25 pages, 2135 KB  
Article
Monitoring Wolfberry (Lycium barbarum L.) Canopy Nitrogen Content with Hyperspectral Reflectance: Integrating Spectral Transformations and Multivariate Regression
by Yongmei Li, Hao Wang, Hongli Zhao, Ligen Zhang and Wenjing Xia
Agronomy 2025, 15(9), 2072; https://doi.org/10.3390/agronomy15092072 - 28 Aug 2025
Cited by 1 | Viewed by 1195
Abstract
Accurate monitoring of canopy nitrogen content in wolfberry (Lycium barbarum L.) is essential for optimizing fertilization management, improving crop yield, and promoting sustainable agriculture. However, the sparse, architecturally complex canopy of this perennial shrub—featuring coexisting branches, leaves, flowers, and fruits across maturity [...] Read more.
Accurate monitoring of canopy nitrogen content in wolfberry (Lycium barbarum L.) is essential for optimizing fertilization management, improving crop yield, and promoting sustainable agriculture. However, the sparse, architecturally complex canopy of this perennial shrub—featuring coexisting branches, leaves, flowers, and fruits across maturity stages—poses significant challenges for canopy spectral-based nitrogen assessment. This study integrates methods across canopy spectral acquisition, transformation, feature spectral selection, and model construction, and specifically explores the potential of hyperspectral remote sensing, integrated with spectral mathematical transformations and machine learning algorithms, for predicting canopy nitrogen content in wolfberry. The overarching goal is to establish a feasible technical framework and predictive model for monitoring canopy nitrogen in wolfberry. In this study, canopy spectral measurements are systematically collected from densely overlapping leaf regions within the east, south, west, and north orientations of the wolfberry canopy. Spectral data undergo mathematical transformation using first-derivative (FD) and continuum-removal (CR) techniques. Optimal spectral variables are identified through correlation analysis combined with Recursive Feature Elimination (RFE). Subsequently, predictive models are constructed using five machine learning algorithms and three linear regression methods. Key results demonstrate that (1) FD and CR transformations enhance the correlation with nitrogen content (max correlation coefficient (r) = −0.577 and 0.522, respectively; p < 0.01), surpassing original spectra (OS, −0.411), while concurrently improving model predictive capability. Validation tests yield maximum R2 values of 0.712 (FD) and 0.521 (CR) versus 0.407 for OS, confirming FD’s superior performance enhancement. (2) Nonlinear machine learning models, by capturing complex canopy-light interactions, outperform linear methods and exhibit superior predictive performance, achieving R2 values ranging from 0.768 to 0.976 in the training set—significantly outperforming linear regression models (R2 = 0.107–0.669). (3) The Random Forest (RF) model trained on FD-processed spectra achieves the highest accuracy, with R2 values of 0.914 (training set) and 0.712 (validation set), along with an RPD of 1.772. This study demonstrates the efficacy of spectral transformations and nonlinear regression methods in enhancing nitrogen content estimation. It establishes the first effective field monitoring strategy and optimal predictive model for canopy nitrogen content in wolfberry. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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Article
Leaf Area Index Estimation of Grassland Based on UAV-Borne Hyperspectral Data and Multiple Machine Learning Models in Hulun Lake Basin
by Dazhou Wu, Saru Bao, Yi Tong, Yifan Fan, Lu Lu, Songtao Liu, Wenjing Li, Mengyong Xue, Bingshuai Cao, Quan Li, Muha Cha, Qian Zhang and Nan Shan
Remote Sens. 2025, 17(16), 2914; https://doi.org/10.3390/rs17162914 - 21 Aug 2025
Cited by 3 | Viewed by 2320
Abstract
Leaf area index (LAI) is a crucial parameter reflecting the crown structure of the grassland. Accurately obtaining LAI is of great significance for estimating carbon sinks in grassland ecosystems. However, spectral noise interference and pronounced spatial heterogeneity within vegetation canopies constitute significant impediments [...] Read more.
Leaf area index (LAI) is a crucial parameter reflecting the crown structure of the grassland. Accurately obtaining LAI is of great significance for estimating carbon sinks in grassland ecosystems. However, spectral noise interference and pronounced spatial heterogeneity within vegetation canopies constitute significant impediments to achieving high-precision LAI retrieval. This study used hyperspectral sensor mounted on an unmanned aerial vehicle (UAV) to estimate LAI in a typical grassland, Hulun Lake Basin. Multiple machine learning (ML) models were constructed to reveal a relationship between hyperspectral data and grassland LAI using two input datasets, namely spectral transformations and vegetation indices (VIs), while SHAP (SHapley Additive ExPlanation) interpretability analysis was further employed to identify high-contribution features in the ML models. The analysis revealed that grassland LAI has good correlations with the original spectrum at 550 nm and 750 nm–1000 nm, first and second derivatives at 506 nm–574 nm, 649 nm–784 nm, and vegetation indices including the triangular vegetation index (TVI), enhanced vegetation index 2 (EVI2), and soil-adjusted vegetation index (SAVI). In the models using spectral transformations and VIs, the random forest (RF) models outperformed other models (testing R2 = 0.89/0.88, RMSE = 0.20/0.21, and RRMSE = 27.34%/28.98%). The prediction error of the random forest model exhibited a positive correlation with measured LAI magnitude but demonstrated an inverse relationship with quadrat-level species richness, quantified by Margalef’s richness index (MRI). We also found that at the quadrat level, the spectral response curve pattern is influenced by attributes within the quadrat, like dominant species and vegetation cover, and that LAI has positive relationship with quadrat vegetation cover. The LAI inversion results in this study were also compared to main LAI products, showing a good correlation (r = 0.71). This study successfully established a high-fidelity inversion framework for hyperspectral-derived LAI estimation in mid-to-high latitude grasslands of the Hulun Lake Basin, supporting the spatial refinement of continental-scale carbon sink models at a regional scale. Full article
(This article belongs to the Section Ecological Remote Sensing)
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