Search Results (231)

Background: Today, it is important to measure livestock water consumption to devise sustainable solutions that consider environmental issues, livestock health requirements and animal welfare. Methods: This longitudinal study measured the water consumption of 66 calves subjected to two feeding diets: a recommended diet as control (CON) and an optimised diet (OPT). Individual measurements were collected daily and summarised on a weekly basis over a 20-week period. The analysis considered the impact of environmental conditions depending on the season of the calf’s birth. Results: Before weaning, calves spontaneously drank significant amounts of water in addition to the water brought by the calf milk replacer (CMR), but there was variability between animals. Water consumption among calves in the OPT group was higher than that among calves in the CON group from week 4 onwards (p = 0.005). At weaning, there was a significant increase in water consumption with a total water intake higher in calves in the OPT group compared to calves in the CON group (118.4 L and 78.9 L; p < 0.001). After weaning, water consumption was correlated with the solid feed intake in our model, which did not include direct fodder other than straw. There were no seasonal effects on water consumption before weaning at 9 weeks, but effects were observed after 13 weeks on the feeding plan (p = 0.008), with higher water consumption among calves born in winter and exposed to warmer temperatures in spring. Over a 20-week period, when calves had reached a weight of 180 kg in the OPT group and 150 kg in the CON group, water consumption had reached 1602 L and 1400 L respectively (p < 0.001). Conclusions: Free access to water should be maintained in calf rearing facilities, as water contributes to concentrated CMR and dry solid feed assimilation and the welfare of calves when the feeding plan remains at a modest level, enabling them to tolerate fluctuating environmental conditions. Full article

Background: Malnutrition and its risk are prevalent in older adults and contribute to frailty, morbidity, and mortality. Poor oral health—particularly tooth loss and inadequate prosthetic rehabilitation—may impair chewing, limit dietary variety, and accelerate nutritional decline. We investigated associations between dentition status, denture use, and nutritional status in a nationally representative sample of Polish older adults. Methods: We analyzed data from 5214 participants aged ≥60 years from the nationwide cross-sectional PolSenior2 study. Dentition status was classified as functional dentition (≥20 teeth), partial dentition (1–19 teeth), or edentulism (0 teeth). Nutritional status was assessed using the Mini Nutritional Assessment—Short Form (MNA-SF); impaired nutritional status was defined as MNA-SF <12 (malnourished or at risk). Additional indicators included hypoalbuminemia (<35 g/L) and small calf circumference (<31 cm). Associations were tested using chi-square and multivariable logistic regression adjusted for age, sex, education, and place of residence. Results: Functional dentition was present in 15.5%, partial dentition in 48.1%, and edentulism in 36.4% of participants. The proportion of edentulous individuals increased across worsening MNA-SF categories (26.2% in well-nourished, 41.8% in at risk, 46.9% in malnourished). In adjusted models, edentulism was associated with higher odds of impaired nutritional status compared with functional dentition (OR: 1.66; 95% CI: 1.32–2.10; p < 0.001), while partial dentition showed a non-significant trend (OR: 1.22; 95% CI: 0.98–1.52; p = 0.077). Among edentulous participants, denture use was more common in well-nourished individuals than in those with impaired nutritional status (93.0% vs. 77.2%), suggesting a possible association between active prosthetic rehabilitation and better nutritional status. Conclusions: In Polish older adults, tooth loss—particularly edentulism—is associated with poorer nutritional status. Screening for malnutrition risk may benefit from incorporating basic oral health and denture-use assessment while improved access to prosthetic rehabilitation may support nutritional resilience in ageing populations. Full article

A multitude of factors affect the optimum calving season, requiring site-specific systems analysis. The objective of this study was to determine the effects of the calving season (S1 and S2) and the timing of calving within the season on production parameters and economics in southwest Missouri. A five-year study was conducted from 2014 to 2018 using field data recorded for 1979 Hereford cow–calf pairs. Cows were categorized by calving season and 21 d calving periods within season (Periods 1–5) in which they calved. Data were analyzed under a completely randomized design with effects of season, period, calf sex, and two and three-way interactions with the calving year as a random effect. The Cattle Value Discovery System (CVDS) beef cow model was used to estimate required cow feed and feed intake. Historical cattle market prices were used, along with total feed costs, to calculate net returns. Calving in later periods reduced the pregnancy percentage in S1 but not S2. Cows calving earlier in both seasons weaned heavier calves. Feed costs were greater in S2, but replacement heifer costs were greater in S1, especially in later calving periods. Overall, net returns were not different between S1 and S2 cows, but net returns declined in later calving periods in S1, whereas there was no difference in S2. In conclusion, at the latitude of southwest Missouri for a forage species with a bimodal growth curve, the calving period affected cow productivity and profitability in Season 1 but less so in Season 2 cows, with no difference in profitability between calving seasons. Full article

This study aimed to scientifically assess cold stress in dairy calves and optimize winter rearing protocols. A combined approach of feeding trials, expert surveys, and multidimensional data analysis was used to evaluate the effects of outdoor (−5~−28 °C) and indoor (5 °C) environments on Holstein dairy calves. A 60-day controlled trial was conducted with 20 healthy 5-day-old calves. In parallel, an interdisciplinary panel of 20 experts and 8 farmers established a cold stress evaluation system via the analytic hierarchy process (AHP), with cold stress levels quantified through fuzzy comprehensive evaluation. Environmental (weight = 0.62), physiological (weight = 0.22), and behavioral (weight = 0.16) factors contributed differentially to cold stress assessment, with data showing that outdoor calves were under mild cold stress (maximum membership degree = 0.64). The temperature–humidity index (THI) showed significant correlations with multiple physiological and biochemical parameters. Generalized linear mixed model (GLMM) analysis confirmed that THI variation significantly influenced calf standing time, respiratory rate (RR), malondialdehyde (MDA) content, and total antioxidant capacity (T-AOC). In feeding trials, indoor calves exhibited marginally higher average daily gain and body weight in early stages, whereas outdoor calves demonstrated significantly better growth performance by day 60. The outdoor group displayed increased lying and defecation behaviors, along with reduced locomotor/standing time and respiratory frequency. No significant intergroup differences were observed in serum immune or antioxidant indicators. Metabolomic analysis identified 20 differentially expressed metabolites, indicating an enhancement in the activity of energy metabolism pathways in calves. This study establishes a quantitative methodology for cold stress evaluation, clarifies environment–physiology–behavior interactions, and provides a theoretical basis for winter calf management. The results confirm that outdoor cold exposure did not hinder calf growth without compromising health, offering scientific support for optimizing outdoor rearing strategies in cold regions. Full article

This study investigated the associations between acute-phase proteins (APPs) such as serum amyloid A (SAA), haptoglobin (Hp), and albumin (Alb) as well as immunoglobulin G (IgG) with bovine respiratory disease (BRD), seroconversion to respiratory infections and farm-level factors in rearing calves. Datasets were obtained from a randomised trial of 476 calves in Finland that compared morbidity in large- versus small-group housing. Calves were assessed for clinical BRD, and their blood was sampled three times during the first 50 rearing days to measure APPs and IgG concentrations and virus-specific antibodies against Mycoplasma bovis (M. bovis), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus 3 (BPIV3), and bovine coronavirus (BCV). Linear mixed-effects regression models showed higher serum SAA and Hp concentrations in calves with clinical BRD. BRSV seroconversion was associated with increased serum SAA and lower Alb while M. bovis seroconversion with increased serum Hp. Calves in larger groups had lower serum Hp, SAA and Alb, and pens with higher BRD cases were associated with increased serum SAA and lower Alb. IgG concentration was associated with BRSV seroconversion. These results suggest that early immune monitoring using APPs and IgG could help guide targeted management strategies to improve calf health and welfare. Full article

Amid rapid global population aging, developing age-friendly urban spaces centered on the “15-minute community life circle” has become a priority in planning research. Taking Shenhe District of Shenyang City, a region undergoing deep aging, as a case study, this research constructs a facility weighting system reflecting the actual needs of the elderly. Integrating multi-source spatial data, the XGBoost model and SHAP framework were applied to analyze the non-linear effects of socio-economic, functional, and land-use factors on facility convenience. Results indicate that: (1) facility convenience exhibits a distinct “west-high, east-low” spatial pattern, characterized by high agglomeration in the western core and significant deficits in the eastern fringe; (2) convenience levels vary across categories, with medical and health facilities showing the highest accessibility, while cultural and leisure (CALFs), life service, and elderly care service facilities (ECSFs) remain the primary deficiencies; and (3) influencing variables demonstrate complex non-linear mechanisms, wherein functional density and distance from the city center are critical drivers with non-monotonic effects, while road network density displays threshold effects, inhibiting ECSFs and CALFs at high densities. These findings provide a refined, quantitative basis for optimizing facility layouts and formulating urban renewal strategies to build age-friendly communities. Full article

Background/Objectives: The calf muscles are vital for generating propulsive force during walking. This power is produced from calf muscle contractions and elastic strain energy release. However, the impact of walking speed on these power-generation mechanisms is understudied. This study aimed to investigate how different walking speeds affect calf muscle activation and ankle power generation. Methods: In this study, we analyzed electromyography (EMG) signals from the gastrocnemius (GAS) and soleus (SOL) muscles of 55 healthy individuals walking at various speeds. C1: household ambulators (0–0.4 m·s⁻¹), C2: limited community ambulators (0.4–0.8 m·s⁻¹), C3: community ambulators (0.8–1.2 m·s⁻¹), C4: self-selected usual speed, and C5: self-selected fast speed. Results: Deviating from a participant’s self-chosen pace led to increased cumulative muscle activity and prolonged plantar flexor activation. Optimal muscle activation was observed at speeds between 0.8–1.2 m·s⁻¹. A second-degree polynomial mixed model best captured the relationship between muscle activation duration and integrated EMG in the ankle power generation phase in late stance, demonstrating the nonlinear relationship between walking speed and calf muscle activation in this phase. Statistically significant models (p < 0.001) explained over 50% of the variability in GAS activation duration (R² = 0.55) and integrated EMG (R² = 0.56), as well as SOL activation duration (R² = 0.52) and integrated EMG (R² = 0.72). Conclusions: The nonlinear relationship between walking speed and calf muscle activation indicates that normal walking speed optimizes the utilization of elastic strain energy in the ankle power generation phase. Full article

Cow efficiency is vitally important to beef sustainability, and computer simulation models may be useful tools to identify characteristics of the most efficient cow genotypes for a given production environment. The objective of this analysis was to determine whether the Beef Cattle Systems Model could replicate empirical research demonstrating a genotype–nutritional environment interaction for efficiency of feed conversion to calves weaned. Combinations of cow genotypes for lactation potential (8, 10, and 12 kg/d at peak milk) and growth potential (450, 505, and 650 kg mature weight) were simulated across four dry matter intake levels (58, 76, 93, and 111 g/kg BW^0.75). At lower dry matter intakes, cows had lesser body condition scores and weight and longer postpartum intervals, but dry matter intake had minimal influence on pregnancy percentage or calf-weaning weight. These trends match empirical research except for pregnancy percentage, where decreasing dry matter intake had a dramatic effect on pregnancy percentage in high-milking, high-growth-potential genotypes. Efficiency of feed conversion was greatest at low dry matter intake for the model simulation with no evidence of a genotype–dry matter intake interaction, which is in contrast to empirical research demonstrating a genotype–dry matter intake interaction. In conclusion, standard nutrition equations do not replicate the genotype–nutritional environment interaction observed in empirical research studies. Full article

Weaning age is a critical management decision in beef cattle production, influencing herd productivity, financial outcomes, and overall system sustainability. Commonly practiced in South African beef systems, is where calves are weaned at 6–9 months (conventional weaning), while early weaning (EW) at approximately 90 days remains underutilized. This study presents a farm case study and preliminary financial assessment of EW and CW using a farm calculation model incorporating revenue, weaning costs, supplementation, and labor. Data from 152 Bonsmara cow–calf pairs were analyzed. CW calves achieved higher weaning weights (237 kg) and average daily gains (992 g/day) than EW calves (210 kg; 889 g/day), generating greater revenue (R630,420 vs. R558,600). The Pearson Chi-square test showed an association between weaning system and dam reproductive performance, with EW cows achieving a 94% pregnancy rate compared to 84% under CW. Although CW produced higher short-term gross margins (R6446 per system vs. R3068 for EW), sensitivity analyses indicated that EW becomes financially competitive when price premiums are applied. Simulations showed that an EW price range of R34–R40/kg could yield higher returns despite lower weights. These findings demonstrate that EW, when supported by structured price incentives, can enhance reproductive efficiency and contribute to more sustainable and financially resilient beef production systems in South Africa. Full article

To address the low efficiency and stress-inducing nature of traditional manual weighing for dairy cows, this study proposes a semantically guided 3D reconstruction and body weight estimation method for dairy cows. First, a dual-viewpoint Kinect V2 camera synchronous acquisition system captures top-view and side-view point cloud data from 150 calves and 150 lactating cows. Subsequently, the CSS-PointNet++ network model was designed. Building upon PointNet++, it incorporates Convolutional Block Attention Module (CBAM) and Attention-Weighted Hybrid Pooling Module (AHPM) to achieve precise semantic segmentation of the torso and limbs in the side-view point cloud. Based on this, point cloud registration algorithms were applied to align the dual-view point clouds. Missing parts were mirrored and completed using semantic information to achieve 3D reconstruction. Finally, a body weight estimation model was established based on volume and surface area through surface reconstruction. Experiments demonstrate that CSS-PointNet++ achieves an Overall Accuracy (OA) of 98.35% and a mean Intersection over Union (mIoU) of 95.61% in semantic segmentation tasks, representing improvements of 2.2% and 4.65% over PointNet++, respectively. In the weight estimation phase, the BP neural network (BPNN) delivers optimal performance: For the calf group, the Mean Absolute Error (MAE) was 1.8409 kg, Root Mean Square Error (RMSE) was 2.4895 kg, Mean Relative Error (MRE) was 1.49%, and Coefficient of Determination (R²) was 0.9204; for the lactating cows group, MAE was 12.5784 kg, RMSE was 14.4537 kg, MRE was 1.75%, and R² was 0.8628. This method enables 3D reconstruction and body weight estimation of cows during walking, providing an efficient and precise body weight monitoring solution for precision farming. Full article

Background/Objectives: Frailty is a common geriatric syndrome associated with adverse outcomes such as disability, hospitalization, and mortality. This study aimed to assess the association between body mass index (BMI) trajectories over ten years and frailty among community-dwelling older adults in Brazil. Methods: This population-based longitudinal study used data from the COMO VAI? cohort, conducted with individuals aged ≥60 years in Pelotas, southern Brazil. Frailty was defined in 2024 using Fried’s phenotype, which evaluates weight loss, exhaustion, low physical activity, slowness, and weakness. BMI categories were defined as underweight (BMI < 22.0 kg/m²), eutrophy (22.0–27.0 kg/m²) and overweight (>27.0 kg/m²). BMI trajectories were identified using group-based trajectory modeling for 789 participants with data from at least two of three assessments (2014, 2019, 2024). Only trajectory groups comprising at least 5% of the sample were retained. Associations of baseline BMI and BMI trajectories with frailty were analyzed using Poisson regression with robust variance, adjusted for confounders and calf circumference. Results: Baseline underweight and overweight prevalence were 9.2% and 56.2%, respectively. Trajectory modeling identified three BMI groups: eutrophic (31.6%), overweight (56.4%), and obesity (12.0%). Obesity emerged as a distinct longitudinal trajectory rather than a baseline BMI category. Underweight did not emerge as a distinct BMI trajectory due to its low prevalence over time. Frailty prevalence in 2024 was 36.5%. Overweight trajectory participants had lower frailty prevalence after ten years (PR = 0.73; 95% CI: 0.54–0.99), while baseline underweight was associated with higher frailty ten years later (PR = 1.73; 95% CI: 1.05–2.84), consistent with the known risk of underweight and the potential protective effect observed in overweight older adults. Conclusions: Baseline underweight increased frailty risk, whereas an overweight trajectory showed a potential protective effect, consistent with the “obesity paradox” in older populations. Full article

Pair housing has been proven to improve calves’ welfare during the pre-weaning phase, potentially promoting positive emotions. Based on the emotional valence hypothesis, according to which positive emotions are mainly processed in the left brain hemisphere, infrared thermography (IRT) may detect changes in eye temperature, particularly thermal asymmetries. This study aimed to evaluate whether pair-housed calves presented ocular thermal asymmetry, compared to individually housed calves during the pre-weaning phase. Fifty-six Friesian female calves from two commercial dairy farms were enrolled and housed either individually or in pairs from birth until 8 weeks of age. IRT images of the lacrimal caruncle of both eyes were collected at 7, 21, 35, and 56 days of age. A linear mixed model tested the effects of housing, farm, year, and timepoint. No significant effect of pair housing on ocular asymmetry emerged, while absolute eye temperatures were significantly higher in pair-housed calves. Moreover, eye temperature declined over time, suggesting reduced arousal and habituation with age. Although the lateralization hypothesis was not confirmed, the study offers insights into IRT for assessing calf emotions and supports further investigation in positive contexts to better explore links between housing, emotional valence, and brain activity. Full article

Background: Cultured human skin explants provide preclinical models to investigate drug delivery and the efficacy of topical treatments for wound healing. However, different culture conditions may affect cell viability, proliferation, and even wound healing. Since animal-derived supplements can influence the investigation of human physiological responses, this study evaluated the effects of non-animal supplements on the ex vivo wound healing process to improve the use of this model for preclinical drug efficacy tests. Methods: In in vitro scratch assays using HaCaT cells and fibroblasts, for media supplemented with normal human serum (NHS), oxygen carriers (OCs) had a positive impact on cell migration, supporting the further evaluation in ex vivo skin culture models. Human skin explants with standardized superficial wounds were cultured in four supplemented media: (i) Dulbecco’s Modified Eagle Medium High Glucose (DMEM) with fetal calf serum (FCS), (ii) DMEM with NHS and OC, (iii) CnT-Prime^TM with NHS and OC, and (iv) EpiLife™ with NHS and an OC. Results: During the 12-day culture, we observed re-epithelialization in all groups with the exception of EpiLife + NHS + OC (with no Ca⁺⁺ supplement). For these samples, starting from day 6, we noticed a loosening of the dermal–epidermal junction and disruption of the upper epidermal layer. Furthermore, an immunohistochemical analysis of extracellular matrix components and remodeling factors, including type I and III collagen, transforming growth factor-β2, and matrix metalloproteinase-9, provided insights into tissue repair dynamics. Conclusions: NHS plus OC is comparable to FCS supplementation and represents a more physiological and ethical alternative. Full article

Background: Sarcopenia is a progressive and multifactorial condition linked to aging, malnutrition, and chronic diseases, presenting significant clinical and public health challenges. Current screening tools vary in complexity and diagnostic accuracy, emphasizing the need for simple, evidence-based predictive models suitable for settings with limited resources. Methods: A cross-sectional study was conducted among community-dwelling older adults to develop and internally validate hierarchical predictive models for sarcopenia using readily available primary care variables. Three models were built: (1) a basic clinical model (age, sex, BMI, calf circumference, and SARC-F), (2) a model including nutritional status (Mini Nutritional Assessment, MNA), and (3) an extended model adding bioelectrical impedance parameters (phase angle, PhA). Model performance was assessed using AUC, Brier score, Hosmer–Lemeshow test, and decision curve analysis. Results: The parsimonious model demonstrated excellent discrimination (AUC = 0.91) and good calibration (Hosmer–Lemeshow p = 0.36), while the extended model with MNA and PhA achieved the highest overall performance (AUC = 0.95; Brier = 0.064; p = 0.97). Incorporating MNA and PhA enhanced calibration and clinical utility, especially for risk probabilities between 0.10 and 0.40. Internal validation showed minimal optimism and stable coefficients, with BMI, sex, and PhA as consistent predictors. Conclusions: A model combining anthropometric, nutritional, and bioelectrical variables provides high diagnostic accuracy for sarcopenia while remaining practical for clinical use. Its stepwise design facilitates application at various healthcare levels, supporting early detection and targeted interventions in aging populations. Full article

Aim: The present study aimed to evaluate the agreement between calf circumference (CC) and dual-energy X-ray absorptiometry (DEXA) in assessing muscle mass, and to determine how possible discrepancies influence the diagnosis of malnutrition and its relationship with frailty and disability in older adults. Methods: We analyzed cross-sectional data from 1048 adults aged 65 years and older who participated in the 2001–2002 National Health and Nutrition Examination Survey (NHANES). Malnutrition was defined using the Global Leadership Initiative on Malnutrition (GLIM) criteria, and muscle mass was estimated using both DEXA and CC. Agreement between the two assessment methods was tested with Kappa statistics, while multivariable logistic regression models were used to explore the associations between malnutrition (as determined by each method) and frailty or disability, controlling for age, sex, physical activity, polypharmacy, and urinary albumin levels. Results: CC and DEXA-based appendicular skeletal muscle mass (ASM) showed a moderate correlation (r = 0.592). The prevalence of malnutrition was 10.3% when defined by CC and 9.1% when defined by DEXA (κ = 0.635, p = 0.001). In both cases, malnutrition was significantly associated with frailty (OR: 1.56; 95% CI: 1.240, 1.970, p < 0.001), but not with disability. Adjusting for albumin levels did not substantially change these associations. Conclusions: CC and DEXA demonstrate moderate concordance in estimating ASM. While this level of agreement slightly affects malnutrition prevalence estimates, it does not alter the observed relationship between malnutrition and frailty or disability in older adults. Full article