Search Results (11)

A mycological assessment evaluates the technical condition of a building by identifying damage from biological corrosion, which is particularly susceptible to fungal growth and insect attack. Such assessments are crucial for brick and wooden monuments. Accurate mycological assessments provide essential documentation for builders, designers, and cost estimators, indicating both locations of damage and the risks associated with weakened structures. The proper selection of testing locations during tender documentation preparation for historic buildings is critical, as it significantly impacts renovation and modernization costs. This article reviews building defects and testing protocols used in mycological expert opinions for buildings constructed between 1899 and 1900. It lists additional tests necessary during modernization and details costs resulting from incorrect assessments. The findings offer valuable guidance for specialists evaluating mycological hazards. The research revealed that 48% of structural elements in the examined buildings were incorrectly assessed solely through visual inspection, with the majority of errors occurring in lintels and cornices. Repairing these elements proved to be significantly more costly than the initial testing, with lintel repair exceeding excavation costs by over 60 times and cornice repairs by more than 130 times. Full article

Fiber-reinforced mycelium (FRM) composites offer an innovative and sustainable approach to construction materials for architectural structures. Mycelium, the root structure of fungi, can be combined with various natural fibers (NF) to create a strong and lightweight material with environmental benefits. Incorporating NF like hemp, jute, or bamboo into the mycelium matrix enhances mechanical properties. This combination results in a composite that boasts enhanced strength, flexibility, and durability. Natural FRM composites offer sustainability through the utilization of agricultural waste, reducing the carbon footprint compared to conventional construction materials. Additionally, the lightweight yet strong nature of the resulting material makes it versatile for various construction applications, while its inherent insulation properties contribute to improved energy efficiency in buildings. Developing and adopting natural FRM composites showcases a promising step towards sustainable and eco-friendly construction materials. Ongoing research and collaboration between scientists, engineers, and the construction industry will likely lead to further improvements and expanded applications. This article provides a comprehensive analysis of the current research and applications of natural FRM composites for innovative and sustainable construction materials. Additionally, the paper reviews the mechanical properties and potential impacts of these natural FRM composites in the context of sustainable architectural construction practices. Recently, the applicability of mycelium-based materials has extended beyond their original domains of biology and mycology to architecture. Full article

The number of buildings experiencing humidity problems and fungal growth appears to be increasing as energy-saving measures and changes in construction practices and climate become more common. Determining the cause of the problem and documenting the type and extent of fungal growth are complex processes involving both building physics and indoor mycology. New detection and identification methods have been introduced, and new fungal species have been added to the list of building-related fungi. However, the lack of standardised procedures and general knowledge hampers the effort to resolve the problems and advocate for an effective renovation plan. This review provides a framework for building inspections on current sampling methods and detection techniques for building-related fungi. The review also contains tables with fungal species that have been identified on commonly used building materials in Europe and North America (e.g., gypsum wallboard, oriented strand board (OSB), concrete and mineral wool). The most reported building-associated fungi across all materials are Penicillium chrysogenum and Aspergillus versicolor. Chaetomium globosum is common on all organic materials, whereas Aspergillus niger is common on all inorganic materials. Full article

Problems in the degradation and biodegradation of cultural heritage objects exposed or stored in public buildings and museums and of construction materials are caused (between others) by the activity of microorganisms. Biodeterioration can be observed not only at the level of the building materials of museum buildings, but also at the level of materials from which art objects are made (natural or artificial) and is determined by factors such as the chemical composition and nature of the composition material, the microclimate characteristics and exposure objects, but also through the manner and frequency of surface cleaning and housekeeping in museums. Based on this, the present study offers, through classical methods, a qualitative and quantitative identification of microorganisms inside a heritage museum building located in a temperate climate country. The purpose of the work was to determine to what extent the bacteriological microflora inside can directly and indirectly contribute to the health quality of the building’s occupants as well as the degradation of its materials and structures. The results emphasize the presence of some fungi and bacteria, among them Alternaria spp., Aspergillus spp., Penicillium spp., Cladosporium spp., and Botrytis spp. All of the analyzed rooms have a high and very high degree of fungal contamination (between 524 and 3674 UFC/m³), which can represent a danger to both human health and the integrity of the exhibitions. This is more pronounced considering that some of species of fungi identified are associated with sick building syndrome, problems in humans due to harmful exposure to viruses, bacteria, and pathogens, which generate possible symptoms such as rhinorrhea, nasal congestion, hoarseness, coughing, sneezing, and irritability for the personnel and visitors. Full article

The subject matter of this manuscript concerns the analysis and identification of microorganisms that pose a threat to human health and, in particular, mold fungi occurring in historical buildings. Surfaces infected by fungal spores pose a threat to the structure and the health of both visitors to historical buildings and professionals working in them. Research was undertaken to fill in the defects in building partitions with a supplementary layer of biochar in order to eliminate, or partially reduce, the possibility of contamination with and development of harmful mold fungi. In the designed cement mixture, biochar was used as a filler, the task of which was to eliminate the causes that lead to the development of harmful mold fungi. Microbiological analyses of the surface of walls and air in selected buildings were carried out before and after the application of supplementary biochar layers. The inhibitory properties of the material used against the presence and growth of mold fungi were observed. The average number of microorganisms isolated on the tested partitions decreased by between 70 and 100%. As a consequence, the use of this material significantly influenced the air quality of the rooms, which is important for protecting the health of people at work, as well as those visiting historical buildings. Full article

Background and aims: This study prompted by growing evidence of the relationship between the yeast Candida albicans and Crohn’s disease (CD) was intended to assess the effect of a 6-month course of the antifungal fluconazole (FCZ) on post-operative recurrence of CD. Methods: Mycological samples (mouth swabs and stools) and serum samples were collected from 28 CD patients randomized to receive either FCZ (n = 14) or placebo (n = 14) before surgical resection. Serological analysis focused on levels of calprotectin, anti-glycan antibodies, and antibody markers of C. albicans pathogenic transition. Levels of galectin-3 and mannose binding lectin (MBL) involved in C. albicans sensing and inflammation were also measured. Results: 1, 2, 3, and 6 months after surgery, endoscopy revealed recurrence in 5/12 (41.7%) patients in the FCZ group and 5/9 (55.6%) in the placebo group, the small cohort preventing any clinical conclusions. In both groups, surgery was followed by a marked decrease in C. albicans colonization and biomarkers of C. albicans pathogenic transition decreased to non-significant levels. Anti-glycan antibodies also decreased but remained significant for CD. Galectin-3 and calprotectin also decreased. Conversely, MBL levels, which inversely correlated with anti-C. albicans antibodies before surgery, remained stable. Building biostatistical multivariate models to analyze he changes in antibody and lectin levels revealed a significant relationship between C. albicans and CD. Conclusion: Several combinations of biomarkers of adaptive and innate immunity targeting C. albicans were predictive of CD recurrence after surgery, with area under the curves (AUCs) as high as 0.86. FCZ had a positive effect on biomarkers evolution. ClinicalTrials.gov ID: NCT02997059, 19 December 2016. University Hospital Lille, Ministry of Health, France. Effect of Fluconazole on the Levels of Anti-Saccharomyces cerevisiae Antibodies (ASCA) After Surgical Resection for Crohn’s Disease. Multicenter, Randomized, and Controlled in Two Parallel Groups Versus Placebo. Full article

Infestation of interior walls of buildings with fungal mould is a reason for health concern which is exacerbated in energy-efficient buildings that limit air circulation. Both mycological and mycotoxicological studies are needed to determine the potential health hazards to residents. In this paper, a rare case of the occurrence of Stachybotrys chartarum in an apartment building in the Lubuskie Province in Poland has been described. Isolated as the major constituent of a mixed mycobiota, its specific health relevance still needs to be carefully analyzed as its biochemical aptitude for the synthesis of mycotoxins may be expressed at different levels. Therefore, ecotoxicological tests were performed using two bioindicators: Dugesia tigrina Girard and Daphnia magna Straus. D. tigrina was used for the first time to examine the toxicity of S. chartarum. The ecotoxicological tests showed that the analyzed strain belonged to the third and fourth toxicity classes according to Liebmann’s classification. The strain of S. chartarum was moderately toxic on Potato Dextrose Agar (PDA) as a culture medium (toxicity class III), and slightly toxic on Malt Extract Agar (MEA) (toxicity class IV). Toxicity was additionally tested by instrumental analytical methods (LC-MS/MS). This method allowed for the identification of 13 metabolites (five metabolites reported for Stachybotrys and eight for unspecific metabolites). Spirocyclic drimanes were detected in considerable quantities (ng/g); a higher concentration was observed for stachybotryamide (109,000 on PDA and 62,500 on MEA) and lower for stachybotrylactam (27,100 on PDA and 46,300 on MEA). Both may explain the result observed through the bioindicators. Highly toxic compounds such as satratoxins were not found in the sample. This confirms the applicability of the two bioindicators, which also show mutual compatibility, as suitable tools to assess the toxicity of moulds. Full article

Achieving a satisfactory level for indoor environments of historical buildings is an ongoing problem that needs to be solved due to a large demand for deep retrofits in the whole of Europe. The implementation of the indoor environmental quality index (IEQ) to predict an occupant’s satisfaction in thermo-modernized historical buildings is a new concept which is a response to existing needs. In this article, a relevant study is provided with the intention to evaluate the indoor environmental performance of retrofitting effects in historical buildings dating back to the years 1873–1878. Considering the historical character of the buildings, some of the cellar spaces were fitted out with an innovative internal insulation system of mineral sheets based on calcium silicate to prevent water vapor condensation and effectively limit mold growth. The IEQ methodology was applied for retrofitted and non-retrofitted spaces as a comparison. Four essential components of indoor quality are investigated: thermal comfort, indoor air quality, acoustic comfort, and visual quality. The results of sub-component indexes are calculated based on the measured indoor parameters and the specific sensory functions. This paper discusses the results of an indoor environmental analysis including a mycological air quality assessment with the newly developed IAQ_index (fungal air contamination index), total volatile organic compound concentration (TVOC), CO₂, and formaldehyde (HCHO) assessment, the evaluation energy-related thermal comfort, acoustic, and visual quality, of modernized spaces. A questionnaire survey study was additionally carried out among a building’s users intentioned to compare the accounts of satisfaction before and after the retrofitting process and also to compare “subjective” results with the one’s based on in situ tests. The retrofitting approach was proven to be effective in limiting the presence of molds and a significant difference in indoor environmental quality between thermally insulated and uninsulated spaces was observed and discussed. Full article

We present results from a study evaluating the utility of supervised machine learning to classify single particle ultraviolet laser-induced fluorescence (UV-LIF) signatures to investigate airborne primary biological aerosol particle (PBAP) concentrations in a busy, multifunctional building using a Multiparameter Bioaerosol Spectrometer. First we introduce and demonstrate a gradient boosting ensemble decision tree algorithm’s ability to accurately classify laboratory generated PBAP samples into broad taxonomic classes with a high level of accuracy. We then develop a framework to appraise the classification accuracy and performance using the Hellinger distance metric to compare product parameter probability density function similarity; this framework showed that key training classes were sufficiently different in terms of particle fluorescence and morphology to facilitate classification. We also demonstrate the utility of including advanced morphological parameters to minimise inter-class conflation and improve classification confidence, where relying on the fluorescent spectra alone would likely result in misattribution. Finally, we apply these methods to ambient data collected within a large multi-functional building where ambient bacterial- and fungal-like classes were identified to display trends corresponding to human activity; fungal-like classes displayed a consistent diurnal trend with a maximum at midday and hourly peaks correlating to movements within the building; bacteria-like aerosol displayed complex, episodic events during opening hours. All PBAP classes fell to low baseline concentrations when the building was unoccupied overnight and at weekends. Full article

Forest community connections are crucial to ensure forest stewardship and sustainability. We explored the potential of mushrooming to enable such connections in contexts where these connections have been historically broken, alienating local people from forests. Taking the case of the recent devolution of a community forest (baldios) in central Portugal to the local population, we present a five-year pilot project to rework mycology from a mushroom-centered approach to a mushroom-in-baldios approach. Mushrooms were used as an entry-point to connect the forest ecology with the challenges of governance and community building. The devised activities provided an opportunity for people inside and outside the local community to adventure into the woods and find out more about their socio-ecological history, develop communal and convivial relationships and engage in the responsible gathering of wild mushrooms. However, the hosting of mushroomers to know, value and engage with the community forest recovery has constantly worked against the enclosure of mushrooms to provide marketable forms of leisure. The outcome of these activities depends on the relationships established between mushrooms, mycologists, local administrators, commoners and poachers, all operating within a framework that favors the eradication of resources instead of long-term relationships that sustain places. Full article

In an area representative of a moderate climate zone (Lubuskie Province in Poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. Aspergillus versicolor Tiraboschi was often encountered on building partitions (frequency 4: frequently). The ability to synthesize the carcinogenic sterigmatocystin (ST) means that it poses a risk to humans and animals. Biotoxicological tests of biomasses of A. versicolor were conducted in the Microbiological and Toxicological Laboratory, using the planarians Dugesia tigrina (Girard). The obtained results of the tests covered a broad range of toxicity levels of isolated strains: from weakly toxic (100–1000 mg·L⁻³) to potently toxic (1–10 mg·L⁻³). The high-performance liquid chromatography (HPLC) physicochemical method confirmed the ability of A. versicolor strains to synthesize sterigmatocystin. All of the samples of the air-dry biomasses of the fungi contained ST in the range between 0.03 and 534.38 mg·kg⁻¹. In the bio-safety level (BSL) classification A. versicolor belongs to category 1. Additionally, A. versicolor is an allergenic mould. Full article