Search Results (62)

(1) Background: The insect male accessory gland (MAG) produces seminal fluid components crucial for male reproduction, analogous to the mammalian prostate. While some insect MAGs exhibit binucleate epithelial cells for luminal volume plasticity, the diversity of cellular arrangements and their functional implications across insects remain largely unknown. (2) Methods: We investigated the cellular architecture of MAG epithelia in various shore bug species (infraorder Leptopodomorpha, Hemiptera) and their mechanisms of multinucleation and potential MAG volume regulation. (3) Results: The MAG epithelia of shore bugs comprise a small number of large, plastic syncytial cells with varying nuclear numbers. We hypothesize that these syncytia facilitate effective MAG volume expansion post-eclosion. Uniquely, MAG shrinkage involves the localized contraction of limited muscle fibers, unlike the systematic contraction of circular muscles in most other insects. We further describe sequential cell fusion during the nymphal stage as the mechanism of multinucleation. (4) Conclusions: The unique syncytial organization of Leptopodomorpha MAG epithelia represents an evolutionary divergence from typical binucleate or mononucleate structures in other insects; it is likely that this enables distinct mechanisms for reproductive fluid storage and evacuation. This study highlights the evolutionary diversity of male reproductive organ morphology and function within insects. Full article

Harmless crop-associated insect communities are a fundamental part of the agroecosystem. Their potential as a reservoir of natural enemies of pests has encouraged their conservation through the development of low-impact pest management programs. The brown marmorated stink bug, Halyomorpha halys, represents a serious threat to Italian hazelnut production. Laboratory and field experiments confirmed the susceptibility of this pest to the disruption of the obligated symbiotic interaction with gut bacteria, paving the way for the development of the symbiont-targeted control strategy. Here we present the results of a three-year field assessment of symbiont-targeted control in a hazelnut orchard in northwestern Italy. The use of a biocomplex to disrupt symbiont acquisition by H. halys nymphs was compared to the use of lambda-cyhalothrin insecticide. The effects on the local entomofauna were assessed, as were the trend of H. halys population and the damage caused by stink bugs to harvested hazelnuts. The insecticide consistently reduced the insect diversity in the field, while the anti-symbiont biocomplex had no effect. However, the control of the H. halys population and the stink bug-induced damage to hazelnuts varied over the years in the field plot submitted to the symbiont-targeted approach. Our results indicate that the symbiont-targeted control does not interfere with local insect communities. Key aspects for improving the effectiveness of this tactic are discussed. Full article

Lethocerus patruelis (Stål, 1854) is a large aquatic hemipteran and the only European representative of the family Belostomatidae. Commonly known as the giant water bug, this species was historically restricted to the Balkans, Anatolia, and parts of the Middle East, but has exhibited a marked westward and northward range expansion in recent decades. In this study, we report the first confirmed occurrence of L. patruelis on Lesvos Island, in the northeastern Aegean Sea, based on a direct observation made within a wastewater treatment facility. The individual was identified in situ using diagnostic morphological traits and photographed without disturbance. This finding extends the known insular distribution of the species and underscores its capacity to exploit anthropogenically modified aquatic systems. Given the island’s rich mosaic of natural and artificial wetland habitats—including over 200 mapped sites—Lesvos may offer suitable conditions for the establishment of local populations. This record highlights the need for targeted surveys and long-term monitoring across under-sampled insular landscapes. Full article

Bagrada hilaris is an invasive stink bug causing important yield losses in Brassica crops. It originates from India, Southeast Asia, the Middle East and South Africa and is reported as invasive in several southwestern US states, Hawaii, Mexico, Chile and in the Mediterranean islands of Malta and Pantelleria (Italy). In this study, we tested the effects of gamma rays on the longevity, fecundity and fertility of bagrada bugs. We irradiated them at two different stages of their life cycle (fifth-instar nymphs and two-week-old adults). Irradiation at the nymphal stage had a strong impact on female fecundity, with egg numbers approaching zero at a dose of 80 Gy. Similarly, a full suppression of female fertility was achieved at 80 Gy when they were mated with males irradiated as nymphs or as mature adults. For longevity, gamma rays had only a slight impact on adult male and female life span. Due to the evidence of a gregarious phase during the autumn, these results suggest that small-scale SIT-localized applications by massive collections of bagrada bugs at various stages of development during autumn, followed by irradiation and reintroduction to the field, might be a safe and economically sound approach of control. Research is currently underway to evaluate the fitness of sterile males and mating patterns. Further studies in confined-field conditions will be needed. Full article

Prior to 2010, Lygus lineolaris Palisot de Beauvois was a minor pest in North Carolina cotton, Gossypium hirsutum L., but became one of the top pests by 2017. This insect was already a persistent pest in other US cotton-producing regions. Initial work focused on addressing near-term management needs and documenting locally relevant ecology and population dynamics of the pest in North Carolina. Landscape factors were found that correlated with tarnished plant bug incidence. Adult numbers tended to be higher in fragmented fields (more field edges) and nymph numbers were higher near field edges. The minimum required sample for estimating tarnished plant bug populations was studied. Thresholds developed in the Midsouth were proven to be useful in a study between both southeastern Virginia and northeastern North Carolina. Furthermore, an insecticide application sequence was recommended based on efficacy trials, number of expected insecticides, and preserving beneficial insects early in the season. These were evaluated in a study investigating IPM systems approaches. Finally, new management tactics for tarnished plant bug, such as ThryvOn cotton, are being evaluated. As new management strategies and tactics are developed, they will need to be evaluated for their fit in this IPM system. Full article

Objective: The complexity of software systems, with their multifaceted functionalities and intricate source code structures, poses significant challenges for developers in identifying and resolving bugs. This study aims to address these challenges by proposing an efficient bug localization method that improves the accuracy and effectiveness of identifying faulty code based on bug reports. Method: We introduce a novel bug localization approach that integrates a Long Short-Term Memory (LSTM) attention mechanism with top-K code similarity learning. The proposed method preprocesses bug reports and source code files, calculates top-K code similarities using the BM25 algorithm, and trains an LSTM-Attention model to predict the most relevant buggy source code files. Results: The model was evaluated on six open-source projects (Tomcat, AspectJ, Birt, Eclipse, JDT, SWT) and demonstrated significant improvements over the baseline method, DNNLoc. Notably, the proposed approach improved accuracy across all projects, with average gains of 18% in prediction accuracy compared to the baseline. Conclusion: This study highlights the efficacy of combining similarity learning with attention mechanisms for bug localization. By streamlining debugging workflows and enhancing predictive accuracy, the proposed method offers a practical solution for improving software quality and reducing development costs. Full article

One of the important distribution areas of stone pine (Pinus pinea L.), a native tree species of the Mediterranean Basin in Türkiye, is the Kozak Basin. Pine nut production plays an important role in the livelihood of the rural people of the Kozak Basin. However, in recent years, as a result of mining activities, climate change, and damage caused by the alien invasive species, the western conifer seed bug (Leptoglossus occidentalis Heidemann 1910 (Hemiptera; Coreidae), the decrease in cone and seed yield in the basin has reached significant dimensions. This process has caused the local people’s income sources to decrease. In this study, land use and land cover (LULC) changes and population changes in the Kozak Basin were discussed during the process, where changing forest land functions, especially economic effects, triggered vulnerable communities due to various factors such as climate change and insect damage. LULC classes of the Kozak Basin and their changes in three time periods are presented using the maximum likelihood method. In addition, the exponential population growth rates of the local people in three different time periods were calculated and these rates were interpolated in the spatial plane with a Kriging analysis. In conclusion, the responses of vulnerable communities to the cone and seed yield decline in the Kozak Basin are manifested by LULC changes and migration from the basin. Therefore, in the management of P. pinea areas, the creation of regulations within the framework of sustainability understanding regardless of ownership difference, stakeholder participatory approach management, close monitoring of ecological events occurring in the basin, awareness of vulnerable communities, and alternative livelihoods can be supported. Full article

The rapid growth of Internet of Things (IoT) devices has significantly increased reliance on sensor-generated data, which are essential to a wide range of systems and services. Wireless sensor networks (WSNs), crucial to this ecosystem, are often deployed in diverse and challenging environments, making them susceptible to faults such as software bugs, communication breakdowns, and hardware malfunctions. These issues can compromise data accuracy, stability, and reliability, ultimately jeopardizing system security. While advanced sensor fault detection methods in WSNs leverage a machine learning approach to achieve high accuracy, they typically rely on centralized learning, and face scalability and privacy challenges, especially when transferring large volumes of data. In our experimental setup, we employ a decentralized approach using federated learning with long short-term memory (FedLSTM) for sensor fault detection in WSNs, thereby preserving client privacy. This study utilizes temperature data enhanced with synthetic sensor data to simulate various common sensor faults: bias, drift, spike, erratic, stuck, and data-loss. We evaluate the performance of FedLSTM against the centralized approach based on accuracy, precision, sensitivity, and F1-score. Additionally, we analyze the impacts of varying the client participation rates and the number of local training epochs. In federated learning environments, comparative analysis with established models like the one-dimensional convolutional neural network and multilayer perceptron demonstrate the promising results of FedLSTM in maintaining client privacy while reducing communication overheads and the server load. Full article

Biological invasions are partly defined by their ability to spread rapidly within invaded regions, posing threats to biodiversity and local species assemblages. The painted bug, Bagrada hilaris (Hemiptera, Pentatomidae) (Burmeister, 1835), originally described as being from India, is an important pest worldwide, mainly due to the serious economic losses incurred and the large number of zones invaded. Since its initial detection in Chile in 2016, the invasive and destructive paint bug has started its invasion to the east and invaded new areas in South America. Without adequate control methods, the insect may threaten brassica crop production, resulting in yield losses greater than 70% in recently infested areas. The extraordinarily wide range of the B. hilaris invasion has necessitated studies describing its biology and ecology, as well as highlighting the urgent need for effective and long-term management techniques. Hence, thoroughly revising the data on this pest in new areas that have been invaded, as well as in the area of origin, is expected to enable the development of management programs. This review incorporates information on B. hilaris in South America, including the invasion, ecology, and potential management approaches, which may allow for efficient integrated pest management, mainly in Chile. Lastly, researchers are expected to break the bottlenecks of some key issues, offering valuable perspectives for identifying strategies that warrant additional research as potential biological control agents for B. hilaris in South America, utilizing either augmentative or conservation biological control approaches, as well as semiochemicals and cultural practices. Full article

Kiwifruit (Actinidia chinensis) cultivation is expanding worldwide, with China, New Zealand, and Italy being the major producing countries. Halyomorpha halys, the brown marmorated stink bug, is raising serious concerns to kiwifruit cultivation both in China and Italy. This study aimed at improving the chemical control efficacy against this pest by comparing two insecticide spray techniques (a conventional ray atomizer and a trumpet-modified atomizer adapted for localized spray application) in kiwifruit. In fact, kiwifruit is often grown with a ‘pergola’ training system, which may reduce the effectiveness of insecticide penetration into the canopy. Experiments were performed in naturally infested orchards of both Actinidia chinensis var. chinensis ‘Jintao’ and A. chinensis var. deliciosa ‘Hayward’. Furthermore, mesh cages containing H. halys adults were deployed within orchards to assess the insects’ mortality at 1, 3, 7, and 10 days after an insecticide application with two spray techniques during two consecutive seasons. In the cultivar ‘Jintao’, the two systems performed similarly, while in the cultivar ‘Hayward’, an overall significantly higher insect mortality was recorded with the trumpet atomizer (94–100%) compared to the conventional atomizer (59–78%). Crop damage was also evaluated on both cultivars, simulating the grower insecticide applications with the two spray techniques. At harvest, no difference emerged between the spray techniques, which provided a significantly better protection compared to the untreated control (12–17% compared to 33–47% of injured fruits). Further investigations in this direction are needed also considering the restriction of insecticidal active substances ongoing in the European Union and the need to maximize the efficacy of the available tools. Full article

The antennae of the shield bug Graphosoma italicum (Müller, 1766) were examined through scanning and transmission electron microscopy to reveal their general morphology, as well as the antennal sensilla’s distribution, size, and ultrastructure of their dendrites and function. The antennae comprise five antennomeres (one scape, two pedicels, and two flagellomeres). Different lengths of chaetic mechanosensilla (Ch1-Ch4) exist on all antennomeres, and several highly sensitive campaniform sensilla are embedded in the exoskeleton and measure cuticular strain. One pair of peg sensilla, the typical proprioceptive, is only on the proximal edge of the first pedicel and directed to the distal edge of the scapus. The antennal flagellum possesses two subtypes of trichoid and basiconic sensilla, each with one type of coeloconic olfactory sensilla. The distinctive characteristics of G. italicum are also apparent in two subtypes of coeloconic sensilla embedded in different cavities on both antennomeres of the flagellum, probably with a thermo-hypersensitive function. All studied morphological types of the sensilla and their function were supported by ultrastructural elements. The long and thin trichoid sensilla type 2 (TrS2) with an olfactive function was the most abundant sensilla localized on both flagellomeres. The peripheral antennal sensilla system consists of six main types of sensilla divided into twelve subtypes. Full article

Heavy metals play a crucial role in the environment due to their toxicity, persistence, and bioaccumulation ability, which can lead to severe ecological and health risks. This study aimed to investigate the impact of urbanization and agricultural practices on the heavy metal content in the sediments of the Bug River catchment. To this end, 96 surface sediment samples were collected from various points in the Bug River catchment, including from urban, agricultural, and forested areas. The samples for laboratory analysis were collected in July 2018, 2019, and 2020 in the Polish part of the Bug River watershed. Heavy metal (Zn, Pb, Cr, Ni, Cu, Fe, Mn, and Cd) concentrations were determined using atomic absorption spectroscopy (AAS). The geoaccumulation index (I_geo)_, contamination factor (CF), and pollution load index (PLI) were used to assess the degree of sediment contamination. The results indicate higher concentrations of heavy metals in urban sediments, where cadmium concentrations reached up to 2.5 mg/kg, compared to agricultural and forested areas, where concentrations were significantly lower. The average I_geo value for cadmium was 0.24 in agricultural areas and 0.15 in urban areas, suggesting the predominance of anthropogenic influences over natural sources. The highest PLI values were found in urban areas, reaching a maximum of 0.33, indicating higher pollution levels. Statistical analysis revealed that urban emissions and agricultural activities significantly influenced the presence of these metals in the Bug River sediments. This study’s conclusions emphasize that effective river water quality management requires continuous monitoring and an understanding of anthropogenic and natural pollution sources. The results contribute to a better understanding the interactions between human activities and water quality, crucial for planning protection and remediation strategies. Additionally, this study provides critical insights into optimizing pollution management strategies and developing remediation methods, serving local and regional policymakers in planning protective actions. Full article

Serbia has recently begun facing a serious problem with the Brown Marmorated Stink Bug, Halyomorpha halys (Stål), which was first recorded in October 2015. This species belongs to the Pentomidae family and is notorious for causing extensive damage to plants. During the winter, it tends to gather in urban areas, such as houses and different man-made facilities, which has raised concerns among producers and citizens. The population of this species has rapidly increased, causing significant economic damage to cultivated plants. However, despite the alarming situation no natural enemies have yet been identified in Serbia. Therefore, research in 2022 was focused on collecting stink bug eggs to investigate the presence of egg parasitoids. The study identified two foreign Hymenoptera species for the European region, Trissolcus japonicus (Ashmead) and Tr. mitsukurii (Ashmead) (Scelionidae), recorded for the first time in Serbia. Additionally, the list of egg parasitoid species belonging to the Hymenoptera order includes seven local species: Anastatus bifasciatus (Geoffroy), from the Eupelmidae family; Ooencyrtus sp., from the Encyrtidae family; and Telenomus turesis (Walker), Tr. basalis (Wollaston), Tr. belenus (Walker), Tr. colemani (Crawford), and Tr. semistriatus (Nees von Esenbeck), from the Scelionidae family. In total, nine egg parasitoid species were, for the first time, reported as parasitizing H. halys and related species in Serbia. Full article

Software bugs are a noteworthy concern for developers and maintainers. When a failure is detected late, it costs more to be fixed. To repair the bug that caused the software failure, the location of the bug must first be known. The process of finding the defective source code elements that led to the failure of the software is called bug localization. Effective approaches for automatically locating bugs using bug reports are highly desirable, as they would reduce bug-fixing time, consequently lowering software maintenance costs. With the increasing size and complexity of software projects, manual bug localization methods have become complex, challenging, and time-consuming tasks, which motivates research on automated bug localization techniques. This paper introduces a novel bug localization model, which works on two levels. The first level localizes the buggy classes using an information retrieval approach and it has two additional sub-phases, namely the class-level feature scoring phase and the class-level final score and ranking phase, which ranks the top buggy classes. The second level localizes the buggy methods inside these classes using an information retrieval approach and it has two sub-phases, which are the method-level feature scoring phase and the method-level final score and ranking phase, which ranks the top buggy methods inside the localized classes. A model is evaluated using an AspectJ dataset, and it can correctly localize and rank more than 350 classes and more than 136 methods. The evaluation results show that the proposed model outperforms several state-of-the-art approaches in terms of the mean reciprocal rank (MRR) metrics and the mean average precision (MAP) in class-level bug localization. Full article

Due to the complicated and changing circumstances of the sea environment, path planning technology is essential for unmanned surface vehicles (USVs) to fulfill search tasks. In most cases, the location of the underwater target is unknown, so it is necessary to completely cover the search area. In this paper, the global static path is planned using a parallel line scan search. When encountering unknown obstacles, the improved Bug algorithm is used for local dynamic path planning according to the sensor detection information. This paper first sets up the safe expansion area to ensure the safety of the USV during the obstacle avoidance process and optimizes the movement direction considering the operation and behavior characteristics of the USV. To meet the requirement of USV steering, the Bezier curve is used to smooth the path points, which greatly improves the smoothness of the path. In this paper, the multi-mode switching strategy of the Bug algorithm based on obstacle boundary width obtained by the sensor is proposed, which ensures no area omissions and meets the requirement of search area coverage during the process of bypassing obstacles. The simulation results show that the improved Bug algorithm can maintain a safe distance along the obstacle boundary to bypass the obstacle. Moreover, the improved Bug algorithm effectively improves the path oscillation phenomenon of traditional Bug and shortens the path length and operating time. Finally, through the global search path planning simulation and comparison experiments, the effectiveness of the proposed method is verified. Full article