Search Results (7,911)

Consumer acceptability of whole-muscle and ground beef can largely be dictated by organoleptic properties. The objectives of the current study were to evaluate the organoleptic characteristics of ground beef patties manufactured using different forming attachments. Ground beef patties (n = 333/treatment) were randomly allotted to one of four treatments (Cavity, Nozzle, Manual, Guillotine) and subjected to analysis of cooking loss, cooking time, cooked patty shrinkage, internal cooked color, texture profile analysis, Allo–Kramer shear force, and consumer sensory panel. The forming method altered cooking time (p < 0.0001) and cooked patty shrinkage (p < 0.0001) but did not alter cooking loss (p = 0.8116). Instrumental hardness was greatest for beef patties formed using the Cavity method compared to all other forming treatments (p = 0.0002). The forming method did not alter internal cooked color redness (p > 0.5408), yellowness (p > 0.1053) nor red-to-brown (p > 0.4386). Lastly, consumer ratings for tenderness were altered by the forming method (p < 0.0020), but patty forming did not alter (p > 0.05) any other sensory characteristics. Categorizing the changes to the cooked characteristics of ground beef patties altered by forming and manufacturing techniques is important when determining consumer acceptance and quantifying textural differences. Current results indicate that ground beef patties formed using the nozzle method imparted the fewest detrimental changes to physiochemical and sensory traits when compared to all other forming methods. Nozzle-formed patties required the least amount of cooking time, the least instrumental resilience, the least instrumental force to shear, and the greatest perceived tenderness by consumer panelists. Full article

Chestnut brown rot caused by Gnomoniopsis smithogilvyi represents an increasing threat to Castanea sativa production, although its endophytic behaviour and response to pre-harvest management strategies under field conditions remain poorly understood. This study evaluated the occurrence of G. smithogilvyi in different chestnut tissues and assessed the effects of targeted field treatments on pathogen colonisation and fruit quality in commercial orchards located in north-eastern Portugal. Field trials included applications of a biological fungicide (Problad^®), an inorganic micronutrient fertilizer (Fungicrops Bio^®), a sulfur-micronutrient fertilizer (KSC Mix^®), and a potassium-phosphonate chemical fungicide (Fosetyl-Al^®), alongside untreated control orchards. Samples of leaves, branches, burrs, and nuts were subjected to microbiological and molecular analyses, while fruit external quality parameters were also assessed. G. smithogilvyi was detected in all analysed organs, confirming its widespread endophytic occurrence. Treatment effects were more pronounced in reproductive tissues, with Problad^® and Fosetyl-Al^® significantly reducing pathogen incidence in burrs and nuts compared with the control. KSC Mix^® promoted fruit development but did not significantly reduce fungal incidence. Treated orchards generally showed improved fruit quality and lower insect infestation. Phylogenetic analysis confirmed the identity of all isolates as G. smithogilvyi. Overall, the results highlight the complexity of managing chestnut brown rot and support the need for integrated and sustainable disease management strategies. Full article

Follistatin (FST) binds to and neutralizes members of the transforming growth factor-beta (TGF-β) superfamily, thereby regulating diverse physiological processes, including regulation of skeletal muscle, adipose, and bone homeostasis. FST also promotes adipose browning and enhances energy metabolism, leading to improved plasma lipid profiles and metabolic health in mice. Given the emerging association between brown adipose tissue (BAT) activation and reduced atherosclerosis, we investigated the anti-atherogenic potential of FST. Transcriptomic and metabolomic analyses of the Hybrid Mouse Diversity Panel (HMDP) revealed that Fst expression was negatively correlated with aortic lesion area and positively correlated with the expression of multiple adipose browning-associated genes. Adeno-associated viral delivery of Fst (AAV1-FST344) in Ldlr^−/− mice significantly reduced aortic lesion area, improved plasma lipid profiles, and decreased expression of adhesion (VCAM1) and inflammatory (iNOS, TNF-α) markers in white adipose tissue (WAT), liver, and heart. Fst gene delivery also markedly increased uncoupling protein 1 (UCP1) expression in WAT, consistent with WAT browning. Integrated correlation analyses of Fst expression with tissue metabolites, together with plasma metabolite–lesion associations identified in the HMDP, implicated the arginase 1 (Arg1)-mediated metabolic pathway as a key regulator of atherogenesis. Consistent with these findings, Arg1 expression was significantly elevated in WAT, liver, and heart of AAV1-FST344-treated mice and in wild-type versus Fst-knockout mouse embryonic fibroblasts (MEFs). Immunostaining localized Arg1 predominantly to CD68⁺ macrophages in heart and liver. Given recent evidence identifying Arg1 as a novel mediator of efferocytosis, these findings suggest that Arg1 may promote macrophage metabolic reprogramming and resolution of inflammation by enhancing the clearance of apoptotic cells. Furthermore, Fst gene delivery increased the expression of fibroblast growth factor 21 (Fgf21) and adiponectin (AdipoQ) in WAT. Collectively, these findings identify Fst as a novel anti-atherogenic regulator that protects against vascular disease by promoting adipose browning, improving lipid metabolism, and activating Arg1-mediated metabolic pathways. Full article

Background: Vitamin D deficiency rickets is a metabolic bone disorder caused by impaired calcium and phosphate homeostasis resulting from insufficient vitamin D. In children, severe vitamin D deficiency can disturb the mineralization of growing bones and teeth. Although the skeletal manifestations are well recognized, reports describing generalized developmental enamel defects affecting nearly all permanent teeth remain limited. Methods: A 6-year-9-month-old Japanese boy with a history of vitamin D deficiency rickets diagnosed at 2 years 5 months of age was referred to our department for evaluation of generalized discoloration and morphological abnormalities affecting multiple permanent teeth. Clinical, radiographic, and medical findings were reviewed. Results: Laboratory examination at diagnosis revealed severe vitamin D deficiency with elevated intact parathyroid hormone levels. Possible contributing factors included exclusive breastfeeding, delayed weaning, avoidance of fish and dairy products, and limited outdoor activity. Following oral alfacalcidol supplementation, skeletal and biochemical findings gradually normalized. However, clinical examination revealed generalized enamel hypoplasia affecting the permanent incisors and first molars, characterized by yellow-brown discoloration, rough enamel surfaces, morphological irregularities, and attrition, whereas the primary dentition showed no obvious abnormalities. Panoramic radiography demonstrated generalized crown malformation involving both erupted and unerupted permanent teeth, particularly the permanent incisors, first molars, and canines, while premolars and second molars were relatively unaffected. Based on the developmental timing of the affected teeth and the patient’s medical history, the enamel defects were considered to be associated with systemic mineralization disturbance during early childhood. Restorative treatment, including composite resin restorations and stainless steel crowns, was performed to improve aesthetics and occlusal function. Preventive surgical exposure followed by composite resin restoration was also performed for the permanent canines at the onset of eruption. Conclusions: Severe vitamin D deficiency during critical stages of tooth development may be associated with irreversible developmental enamel defects in the permanent dentition, even after apparent systemic recovery from rickets. Early dental assessment, long-term dental follow-up, and multidisciplinary management should be considered in children with a history of nutritional rickets. Full article

Breed and herd are major determinants of milk productivity and efficiency. This study evaluated 1508 lactating cows from three dairy (Holstein, Brown Swiss, Jersey) and three dual-purpose (Simmental, Rendena, Alpine Grey) breeds across 41 multi-breed mountain herds. Data on body size, production, and milk samples were collected. Daily milk yield, milk energy, and cheese yield were quantified, and herds were classified under high (>75 MJ/d) or low (<75 MJ/d) average daily milk energy production. Cheese-making traits were predicted from 508 individual model cheese-making trials. Nine productivity ratios and three efficiency indicators (energy efficiency, economic efficiency, and income over feed costs) were calculated. Data was analyzed using mixed models including herd productivity class, herd within class (random), breed, parity, and days in milk. Differences among breeds were smaller within herds than across herds and were more pronounced for quality and cheese-making traits. Dairy and dual-purpose breeds showed similar overall performance. Jerseys were the least productive in absolute terms but, when adjusted for body size, were as or more efficient than other dairy breeds. Holsteins had the highest milk yield, whereas Brown Swiss showed superior milk quality and cheese yield. Simmental outperformed local dual-purpose breeds in size and production but not in efficiency. Accurate assessment of energy and economic efficiency is essential for breeding and crossbreeding strategies. Full article

Marine medicinal algae represent a valuable reservoir of bioactive metabolites for anticancer drug discovery, yet the efficient identification of target-relevant compounds from chemically complex marine matrices remains challenging. In this study, an integrated cathepsin B-oriented strategy was developed to discover, prioritize, isolate, and validate antitumor metabolites from the brown alga Sargassum polycystum. Affinity ultrafiltration LC-MS was first applied to screen CTSB-binding constituents from the crude extract, followed by molecular docking, molecular dynamics simulation, and gray relational analysis for multidimensional candidate prioritization. Seven CTSB-binding metabolites were characterized, including chlorogenic acid, caffeic acid, cynarin, loliolide, taxifolin, senkyunolide H, and dihydroactinidiolide, with binding degrees of 73.99–85.61% at 2.5 U/mL CTSB. Molecular docking showed predicted binding affinities ranging from −6.3 to −9.4 kcal/mol, compared with −10.2 kcal/mol for the positive control CA-074Me. Integrated computational and biological evaluation identified caffeic acid, cynarin, and taxifolin as the top-ranked candidates. Preparative recovery was then achieved using counter-current chromatography combined with semi-preparative HPLC, and the isolated compounds were structurally identified by LC-MS/MS and NMR. Cellular assays in NCI-H1975 cells suggested that these metabolites reduced CTSB-associated enzymatic activity and intracellular CTSB-related fluorescence signals to different extents, with phenolic acid-type compounds exhibiting comparatively stronger effects. At the extract level, S. polycystum dose-dependently suppressed NCI-H1975 xenograft tumor growth, with inhibition rates of 48.78%, 36.58%, and 22.86% in the high-, middle-, and low-dose groups, respectively, without evident hepatorenal histopathological toxicity. This effect was associated with reduced CTSB, Ki-67, and Bcl-2 staining, increased Bax staining, enhanced apoptosis, and ultrastructural alterations in tumor tissues. Overall, this study provides a practical CTSB-oriented workflow for discovering antitumor metabolites from marine medicinal algae and supports further investigation of S. polycystum as a potential source of anti-NSCLC candidates. Full article

Edible and medicinal mushrooms, including cultivated and wild species, are increasingly recognized as valuable functional foods and nutraceutical resources due to their high nutritional value, abundance of bioactive compounds, and documented health-promoting properties. However, their high perishability results in substantial postharvest quality losses and limits commercial shelf life. This review provides a comprehensive overview of recent advances in mushroom preservation technologies, with particular emphasis on emerging non-thermal approaches such as cold plasma treatment, active packaging systems, and electrostatic field technologies. Conventional and advanced drying methods, edible coatings, biopreservation, fermentation and irradiation are also critically evaluated. Cold plasma treatment effectively reduces microbial contamination and enzymatic browning while maintaining firmness and nutritional quality, whereas active packaging systems based on chitosan films, nanocomposites, and modified atmospheres help reduce moisture loss, delay senescence, and preserve physicochemical properties during storage. Electrostatic field treatment combined with modified atmosphere packaging has shown additional potential for extending refrigerated shelf life. Among drying technologies, freeze-drying generally provides the highest retention of colour, texture and bioactive compounds, although its industrial application remains constrained by high energy consumption and operational costs. Overall, current evidence suggests that integrated preservation approaches offer the greatest potential for improving shelf-life extension and quality retention. Nevertheless, further research is required to address challenges related to industrial scalability, process standardization, economic feasibility and long-term quality assessment. Full article

Background/Objectives: This study aimed to assess access to dental services by people living with HIV/AIDS in Bahia, Brazil, and its associated factors. Methods: A cross-sectional study was carried out with a convenience sample involving 145 people living with HIV/AIDS in Bahia, recruited from an HIV/AIDS referral center and members of two social organizations. Results: Most participants were cisgender men (41.8%), lived in the capital (80.0%), and had a mean age of 44 years (±11.8). More than half had visited a dentist within the previous year (56.2%), mainly for routine or maintenance care (52.4%). Non-disclosure of HIV serological status to dentists was reported by 63.6% of participants and was significantly associated with lower educational attainment (p = 0.03). Never having attended a dental appointment was significantly more frequent among black and brown individuals (p = 0.04), non-binary participants (p = 0.03), and those with fewer years of schooling (p = 0.008). Conclusions: The results showed inequality in access to oral health care among people with HIV/AIDS in Bahia, influenced by factors such as race, schooling, and gender. Despite economic, geographical, and structural barriers, more than half of the participants had consulted a dentist in the past year and received guidance on the disease’s oral manifestations. Full article

The non-specific lipid transfer proteins (nsLTPs) are able to bind various hydrophobic compounds and facilitate the transport of fatty acids between intracellular membranes, and nsLTPs are found in rice endosperm and embryo during seed development. However, whether nsLTPs function as lipid carriers and thereby affect lipid metabolism in rice grains remains unclear. To elucidate whether nsLTPs influence fatty acid distribution in rice, we generated OsLTP10-OE (OsLTP10 overexpression) and OsLTP10-CR (OsLTP10 CRISPR/Cas9) lines. Phenotypic and metabolic analyses indicated that OsLTP10 expression is closely associated with fatty acid (FA) profiles and grain appearance. In general, total fatty acid content in the brown rice of OsLTP10-OE was higher than that in wildtype, but OsLTP10-CR was lower than wildtype. While FA accumulation was altered in both tissues, the endosperm (milled grain) was more severely affected than the bran, with individual FAs in the milled grains of OsLTP10-OE expanding by 31.87–52.00%. Additionally, key grain quality traits were substantially altered; OsLTP10-CR lines displayed a significantly enlarged white-belly chalkiness area alongside a 19.50% reduction in amylose content, whereas OsLTP10-OE lines showed decreased chalkiness and a 7.80% increase in amylose. Overall, the fatty acid content and composition, chalkiness, brown rice size, and amylose were influenced by OsLTP10. Full article

Matcha tea (Camellia sinensis) contains high levels of catechins but has a near-neutral pH (6.2–6.3), which limits the stability of its bioactive compounds. Blending matcha with acidic berries may enhance phenolic stabilization, antioxidant capacity, and sensory properties. This study evaluated pure matcha (MT) and a matcha beverage blended with 7.5% strawberry and 7.5% blackberry powder (Mix), extracted at 5 °C, 70 °C, and 100 °C. Sensory evaluation using a 9-point hedonic scale (n = 50) identified 15% berry substitution (Mix) as optimal, with overall acceptability scores ranging from 8.28 to 8.32 across all extraction temperatures. Sensory evaluation using a 9-point hedonic scale (n = 50) identified 15% berry substitution (Mix) as optimal. Total phenolics (Folin–Ciocalteu), flavonoids (AlCl₃), anthocyanins (pH differential), vitamin C (HPLC), and individual phenolic compounds were analyzed. Antioxidant activity (DPPH, ABTS) and anti-inflammatory activity (egg albumin denaturation) were assessed, and color parameters (L*, a*, b*, chroma, hue, browning index, ΔE) were measured. Results and Discussion: The Mix exhibited significantly higher total phenolics (24.4% increase at 100 °C) and flavonoids (31.6% increase at 100 °C) compared to MT. Anthocyanins, absent in MT, reached 52.35 mg/100 g at 5 °C, and vitamin C content was 2.6-fold higher than MT under cold extraction. HPLC profiling showed increased levels of gallic acid, protocatechuic acid, catechin, epicatechin, epicatechin gallate, and rutin in the Mix. The Mix demonstrated superior antioxidant activity with DPPH inhibition of 84.08% at 100 °C (IC₅₀ = 165.0 µg/mL) and ABTS inhibition of 83.67% at 100 °C (IC₅₀ = 105.1 µg/mL). Anti-inflammatory activity was highest at 70 °C (IC₅₀ = 72.2 µg/mL), representing a 3.5-fold improvement over MT. Color parameters were similar to MT at 5 °C and 70 °C but darkened at 100 °C. The acidic pH (~3.7) of the Mix remained stable, contributing to catechin stabilization. Conclusion: The 15% strawberry-blackberry matcha blend shows potential as a functionally enhanced beverage with improved phenolic content, vitamin C, anthocyanins, and bioactivities. Temperature selection allows customization: 100 °C for maximal antioxidant activity, 70 °C for anti-inflammatory benefits, and 5 °C for nutrient preservation and vibrant color. Full article

Saccharina japonica is an economically important stenohaline brown seaweed whose growth and yield are significantly affected by frequent salinity fluctuations in coastal aquaculture areas. The differences in salt tolerance and response characteristics among developmental stages and among tissue regions of adult-stage thalli remain unclear, and the dynamic temporal patterns of responses across stages and tissues have not been systematically elucidated. In this study, we compared the physiological responses of juvenile and adult-stage thalli under varying salinity conditions and further analyzed the responses of the basal, middle, and tip regions of adult-stage thalli to define stage- and tissue-specific patterns of salt tolerance. The results indicate that low-salinity stress caused more severe injury than high-salinity stress, as reflected by sustained decreases in Fv/Fm, increased accumulation of MDA, and aggravated tissue decay with green-rot symptoms. Juvenile sporophytes exhibited higher salt tolerance than adult-stage thalli, and within the latter, tolerance differed markedly among tissue regions, with the basal region showing greater tolerance than the middle and tip regions. The basal region maintained higher photosynthetic activity, lower lipid peroxidation levels, and more stable antioxidant and osmotic regulatory responses under stress, whereas the tip region experienced early photosynthetic inactivation and irreversible damage. qRT-PCR results showed that antioxidant- and osmotic-regulation-related genes, including SjGSH, SjGST, SjPro, SjSOD, and SjPOD, were differentially expressed under salinity treatments at 24 h and 72 h, and their expression dynamics were generally consistent with the changes in physiological indicators. Overall, this study demonstrates that the response of S. japonica to salinity stress exhibits clear developmental stage-dependent differences and tissue-specific characteristics. In adult-stage thalli, the tip region may serve as a sensitive monitoring region for low-salinity damage, the middle region may serve as a transitional region for evaluating the progression of stress-induced damage, and the basal region may be an important region for maintaining thallus growth and physiological homeostasis. This study also provides experimental evidence for low-salinity stress risk assessment, the management of key growth stages, the monitoring of sensitive tissues, and the evaluation of salt tolerance traits during S. japonica aquaculture. Full article

Escherichia coli is increasingly recognised as an important cause of ventilator-associated pneumonia (VAP), particularly in intensive care units (ICUs) with high antimicrobial selective pressure. Unlike classical respiratory pathogens, ICU-associated E. coli often originates from the patient’s intestinal microbiota and harbours a complex mobilome enriched with antimicrobial resistance determinants. In this study, a total of 200 nosocomial endotracheal aspirate samples were aseptically collected from patients admitted to the Respiratory ICU at Assiut University hospital. Antimicrobial susceptibility testing, serotyping and screening for various virulence and antimicrobial resistance genes (e.g., extended-spectrum β-lactamase (ESBL) and carbapenems genes) were carried out. In total, E. coli isolates were recovered from 54/200 (27%) endotracheal aspirates, with a high prevalence of multidrug resistance (MDR) observed (74.1%). Resistance to β-lactams was common with phenotypic evidence suggestive of ESBL production detected in 64.8% of isolates. Genome sequencing of three MDR E. coli isolates confirmed that they carried multiple antimicrobial resistance genes, which included ESBL genes (e.g., bla_CTX-M-15 and bla_TEM-1B). Each strain was also found to be high-risk extraintestinal pathogenic E. coli (ExPEC) clones, belonging to either sequence type ST131 or ST405. These findings support an endogenous infection model for VAP, whereby ICU selective pressure favours highly mobile, multidrug-resistant E. coli lineages adapted for extraintestinal survival. The high production of ESBLs and the prevalence of carbapenemase genes highlight the urgent need for molecular surveillance and antimicrobial stewardship strategies for the control of such high-priority pathogens in this part of the world. Full article

Smart city mobility is increasingly governed by a techno-solutionist logic that prizes data, automation, and efficiency, often at the expense of public trust, social legitimacy, and lived experience. This article argues that the fate of a mobility transition appears to depend less on the sophistication of the technology than on the pace and posture of change. Building on the CalmMobility framework and on Weiser and Brown’s concept of calm technology, it develops the idea of calm smart mobility—a human-paced, options-first approach in which innovation enters everyday life gradually and with credible alternatives already in place, so that residents are not asked to continuously adapt. The framework’s three pillars (Comprehensiveness; Pacing–Sequencing–Inclusion; Future-Readiness) are mapped onto four recurring challenges of smart mobility (Policy Layering, Affective Mismatch, Governance Silos, and the Future-Readiness Gap) and then used as a descriptive analytical lens to characterize seven documented implementations across economic, spatial, mass-transit, service, and platform interventions and four world regions: the Stockholm congestion charge, the London ULEZ expansion, the Barcelona superblocks, Bogotá’s TransMilenio bus rapid transit and Ciclovía, Seoul’s Cheonggyecheon restoration and bus reform, Helsinki’s Whim Mobility-as-a-Service, and Sidewalk Toronto. Presented through a comparison table, a positioning map, and adoption trajectories rather than rankings, the characterization suggests that the provision of alternatives, the sequencing and pace of change, and the genuineness of co-creation are more closely associated with smooth adoption than the type of instrument deployed. The article is conceptual and framework-building. The cases illustrate and probe the framework instead of validating it, and a testable central hypothesis is specified for future empirical work. Calm smart mobility is offered as a transferable, citizen-centred logic for guiding smart city mobility transitions at a human pace. Full article

Skeletal deterioration is a major welfare and production concern in laying hens, as substantial quantities of calcium are mobilized from bone to support eggshell formation during the laying cycle. Nutritional strategies that promote skeletal development during the pullet phase may therefore improve bone integrity later in production. The objective of this study was to evaluate the effects of dietary genistein supplementation on growth performance, bone development, and mineralization in Hy-Line Brown pullets. A total of 600 pullets were randomly assigned to four dietary treatments consisting of a control diet (0 mg/kg genistein; CON) or diets supplemented with 20 (G20), 60 (G60), or 100 mg/kg (G100) genistein from 5 to 17 weeks of age. Growth performance, bone mineral density, muscle deposition, biomechanical strength, bone ash content, and circulating bone formation biomarkers were evaluated. Pullets receiving G60 and G100 supplementation exhibited greater body weight, bone mineral density, cortical bone area, muscle weights, biomechanical strength, bone ash percentage, and circulating concentrations of BALP and P1NP compared with CON and G20 birds. Feed intake did not differ among treatments. These findings indicate that genistein supplementation, particularly at 60 and 100 mg/kg, may enhance skeletal development during the pullet rearing period. Further research is warranted to determine whether these improvements persist throughout the laying cycle and influence production, as well as the potential deposition of genistein-derived compounds in eggs. Full article

Agricultural by-products are increasingly recognized as sustainable resources for mushroom cultivation and biomass valorization. This study evaluated pineapple stubble and coffee pulp as functional substrate supplements for Cordyceps militaris cultivation. Fruiting bodies were successfully produced on brown rice substrates supplemented with 5–15% pineapple stubble or coffee pulp, either alone or in combination. Substrate composition markedly influenced fungal morphology, biological efficiency (BE), and cordycepin production. Moderate supplementation improved cultivation performance, whereas excessive supplementation reduced BE. Cordycepin accumulation was generally higher in the substrate than in the fruiting bodies. The highest cordycepin content was observed in the substrate supplemented with 15% coffee pulp, reaching 3.93%, approximately twofold higher than that of the brown rice control. Correlation analysis revealed that carbon content and the carbon-to-nitrogen (C/N) ratio were positively associated with BE, while nitrogen content was positively correlated with cordycepin accumulation in the substrate. In addition, coffee pulp extract exhibited notable adenosine deaminase inhibitory activity, suggesting that substrate-derived bioactive compounds may contribute to enhanced cordycepin accumulation during cultivation. After cultivation, the spent mushroom substrate showed reduced C/N ratios and increased nitrogen content, indicating active fungal metabolism-mediated substrate transformation. These findings demonstrate that coffee and pineapple by-products can serve as sustainable supplements to improve cordycepin production in C. militaris. Furthermore, the resulting spent substrate, enriched in nitrogen and bioactive compounds, may represent a valuable secondary resource for agricultural and industrial applications. Full article