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28 pages, 1433 KB  
Article
The Double-Edged Sword of Dynamic Pricing: Bidirectional Modal Shift and Carbon Leakage in High-Speed Rail
by Zhibin Xing, Chenghao Xing and Xinyu Gou
Sustainability 2026, 18(6), 2802; https://doi.org/10.3390/su18062802 - 12 Mar 2026
Viewed by 540
Abstract
While pricing policy has emerged as a critical demand-side lever for decarbonizing mobility, its bidirectional effects on modal shift remain unexplored. Dynamic pricing in high-speed rail (HSR) creates a double-edged environmental outcome: advance discounts attract passengers from aviation, yet last-minute premiums may reverse [...] Read more.
While pricing policy has emerged as a critical demand-side lever for decarbonizing mobility, its bidirectional effects on modal shift remain unexplored. Dynamic pricing in high-speed rail (HSR) creates a double-edged environmental outcome: advance discounts attract passengers from aviation, yet last-minute premiums may reverse these gains. Using 2.4 million price observations from Madrid–Barcelona (2019), we introduce a carbon leakage framework that quantifies this phenomenon within a multi-source validated framework. Our analysis reveals a structural tension: while early-bird pricing attracts 274,431 annual passengers from aviation—saving 23,650 tonnes CO2/year—last-minute scarcity premiums systematically drive passengers back to air travel. Multi-source calibrated elasticity (ε=0.95, validated through triangulation across CNMC corridor data, meta-analytic evidence, and recent empirical studies within the range [1.91,0.75]) shows that 22.3% of last-minute tickets exceed the EUR 120 aviation threshold, creating 1511 tonnes CO2 leakage annually (6.4% offset of gross savings). Critically, this leakage ratio is shown to be structurally independent of elasticity specification, being determined by the price distribution shape rather than demand parameters. Scenario analysis suggests that under static assumptions, price caps at EUR 110–120 would eliminate leakage while preserving an estimated 94% of operator revenue, though general equilibrium effects remain unmodeled. These findings identify illustrative scenario thresholds for carbon-aware revenue management, demonstrating that demand-side decarbonization requires not only attracting passengers to sustainable modes but also preventing their reversal to high-carbon alternatives. Full article
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12 pages, 593 KB  
Article
Shape, Size and Bilateral Asymmetry of the Humerus and Femur in the Common Swift (Apus apus)
by Eylem Bektaş Bilgiç, Edyta Pasicka, Aycan Korkmazcan, Nicoleta Manuta, Buket Çakar, Ebuderda Günay, Gökhan Gün and Ozan Gündemir
Animals 2025, 15(23), 3401; https://doi.org/10.3390/ani15233401 - 25 Nov 2025
Viewed by 959
Abstract
Common swifts are extreme aerial specialists that spend most of their lives in flight and use their legs mainly for clinging rather than locomotion. Because functional load is therefore expected to be concentrated on the wing, we hypothesized that the forelimb would exhibit [...] Read more.
Common swifts are extreme aerial specialists that spend most of their lives in flight and use their legs mainly for clinging rather than locomotion. Because functional load is therefore expected to be concentrated on the wing, we hypothesized that the forelimb would exhibit stronger left–right differentiation than the hindlimb. In addition to testing this hypothesis, we used landmark-based geometric morphometric methods to describe humeral and femoral shape in common swifts and to test the effects of sex and body size on bone morphology. The humerus showed clear directional asymmetry: the effect of side explained 13.8% of total shape variance (F = 42.0, p = 0.001), indicating a consistent left–right shift across individuals. The femur also exhibited significant but weaker directional asymmetry, with side accounting for 5.4% of variance (F = 19.3, p = 0.001). In both bones, the individual term explained the largest proportion of variation, whereas residual variance (containing fluctuating asymmetry and measurement error) was moderate (≈27% in the humerus, ≈23% in the femur). Allometric regressions showed a weak but significant size–shape relationship for the humerus and only a marginal effect for the femur, and males and females showed almost complete overlap in the distribution of humeral and femoral shapes. Sex had no detectable effect on humeral or femoral shape or asymmetry, and body size explained only a modest proportion of shape variation in both elements. Overall, our results support the functional expectation: the more intensively used forelimb element is also the more directionally asymmetric one, whereas the femur of this largely aerial bird remains comparatively more symmetrical. Full article
(This article belongs to the Special Issue Recent Advances in Veterinary Anatomy and Morphology)
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26 pages, 4887 KB  
Article
Quantitative Assessment of CFD-Based Micro-Scale Renovation of Existing Building Component Envelopes
by Yan Pan, Lin Zhong and Jin Xu
Biomimetics 2025, 10(11), 733; https://doi.org/10.3390/biomimetics10110733 - 1 Nov 2025
Cited by 1 | Viewed by 886
Abstract
With the acceleration of urbanization, environmental degradation is increasingly restricting the improvement of residents’ quality of life, and promoting the transformation of old communities has become a key path for sustainable urban development. However, existing buildings generally face challenges, such as the deterioration [...] Read more.
With the acceleration of urbanization, environmental degradation is increasingly restricting the improvement of residents’ quality of life, and promoting the transformation of old communities has become a key path for sustainable urban development. However, existing buildings generally face challenges, such as the deterioration of the performance of the envelope structure and the rising energy consumption of the air conditioning system, which pose a serious test for the realization of green renovation. Inspired by the application of bionics in the field of architecture, this study innovatively designed five types of bionic envelope structures for outdoor air conditioning units, namely scales, honeycombs, spider webs, leaves, and bird nests, based on the aerodynamic characteristics of biological prototypes. The ventilation performance of these structures was evaluated at three scales—namely, single building, townhouse, and community—under natural ventilation conditions, using a CFD simulation system. The study shows the following: (1) the spider web structure has the best comprehensive performance among all types of enclosures, which can significantly improve the uniformity of the flow field and effectively eliminate the low-speed stagnation area on the windward side; (2) the structure reorganizes the flow structure of the near-wall area through the cutting and diversion of the porous grid, reduces the wake range, and weakens the negative pressure intensity, making the pressure distribution around the building more balanced; (3) in the height range of 1.5–27 m, the spider web structure performs particularly well at the townhouse and community scales, with an average wind speed increase of 1.1–1.4%; and (4) the design takes into account both the safety of the enclosure and the comfort of the pedestrian area, achieving a synergistic optimization of function and performance. This study provides new ideas for the micro-renewal of buildings, based on bionic principles, and has theoretical and practical value for improving the wind environment quality of old communities and promoting low-carbon urban development. Full article
(This article belongs to the Special Issue Biologically-Inspired Product Development)
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18 pages, 2152 KB  
Article
Development and Laboratory Validation of Rapid, Bird-Side Molecular Diagnostic Assays for Avian Influenza Virus Including Panzootic H5Nx
by Matthew Coopersmith, Remco Dijkman, Maggie L. Bartlett, Richard Currie, Sander Schuurman and Sjaak de Wit
Microorganisms 2025, 13(5), 1090; https://doi.org/10.3390/microorganisms13051090 - 8 May 2025
Cited by 4 | Viewed by 6661
Abstract
Avian influenza A viruses (AIV) significantly impact both animal and human health. Reliable diagnostics are crucial for controlling AIV, including the highly pathogenic strains like H5Nx. In this study, we developed and validated the on-site Alveo Sense Poultry Avian Influenza Tests to rapidly [...] Read more.
Avian influenza A viruses (AIV) significantly impact both animal and human health. Reliable diagnostics are crucial for controlling AIV, including the highly pathogenic strains like H5Nx. In this study, we developed and validated the on-site Alveo Sense Poultry Avian Influenza Tests to rapidly detect the AIV M-gene and subtypes H5, H7, and H9 in unprocessed samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and impedance-based measurements. The Alveo Sense tests, using single-use microfluidic cartridges, deliver results within 45 min. Each cartridge includes assays for the AIV M gene and specific H5 and H7 or H9 subtypes, with internal process controls. The laboratory validation involved specificity, limit of detection (LoD), diagnostic sensitivity, reproducibility, and robustness tests using various AIV strains, other avian pathogens, and field samples. The assays showed 100% specificity for AIV subtypes without cross-reactivity with non-AIV pathogens. The LoD95 for H5, H7, and H9 ranged between RT-PCR Ct values of 29–33 in both cloacal and oropharyngeal samples and were able to detect avian influenza virus in both spiked samples and field samples. Reproducibility and repeatability studies showed perfect agreement across operators and laboratories and remained stable and accurate under different pre-analytical conditions. The Alveo Sense tests offer rapid, accurate, and reliable on-site diagnostics for AIV subtypes H5, H7, and H9 on samples from fresh dead and sick birds, valuable for early flock-level detection and outbreak control. Further field validation will improve the understanding of their diagnostic performance across various avian species. Full article
(This article belongs to the Section Virology)
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18 pages, 4666 KB  
Article
A Novel Lateral Control System for Autonomous Vehicles: A Look-Down Strategy
by Farzad Nadiri and Ahmad B. Rad
Machines 2025, 13(3), 211; https://doi.org/10.3390/machines13030211 - 6 Mar 2025
Cited by 2 | Viewed by 3623
Abstract
This paper introduces a robust yet straightforward lane detection and lateral control approach via the deployment of a dual camera based on the look-down strategy for autonomous vehicles. Unlike traditional single-camera systems that rely on the look-ahead methodology and a single front-facing preview, [...] Read more.
This paper introduces a robust yet straightforward lane detection and lateral control approach via the deployment of a dual camera based on the look-down strategy for autonomous vehicles. Unlike traditional single-camera systems that rely on the look-ahead methodology and a single front-facing preview, the proposed algorithm leverages two downward-facing cameras mounted beneath the vehicle’s driver and the passenger side mirror, respectively. This configuration captures the road surface, enabling precise detection of the lateral boundaries, particularly during lane changes and in narrow lanes. A Proportional-Integral-Derivative (PID) controller is designed to maintain the vehicle’s position in the center of the road. We compare this system’s accuracy, lateral steadiness, and computational efficiency against (1) a conventional bird’s-eye view lane detection method and (2) a popular deep learning-based lane detection framework. Experiments in the CARLA simulator under varying road geometries, lighting conditions, and lane marking qualities confirm that the proposed look-down system achieves superior real-time performance, comparable lane detection accuracy, and reduced computational overhead relative to both traditional bird’s-eye and advanced neural approaches. These findings underscore the practical benefits of a straightforward, explainable, and resource-efficient solution for robust autonomous vehicle lane-keeping. Full article
(This article belongs to the Special Issue Trajectory Planning for Autonomous Vehicles: State of the Art)
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22 pages, 9847 KB  
Article
Protection of Passeriformes Birds in Wetland Ecological Restoration: A Case Study of the Reed Parrotbill (Paradoxornis heudei) in Baiyangdian
by Qi Sun, Heng Wu, Taijun Zuo, Zengrui Tian, Jiaojiao Wang and Jianhua Hou
Diversity 2025, 17(1), 75; https://doi.org/10.3390/d17010075 - 20 Jan 2025
Cited by 2 | Viewed by 2529
Abstract
Due to the increasing impact of human activities on the environment, habitat loss, fragmentation, and degradation pose significant threats to bird diversity worldwide. Baiyangdian, the largest freshwater lake wetland in North China, is an important habitat for birds. The degradation of water quality [...] Read more.
Due to the increasing impact of human activities on the environment, habitat loss, fragmentation, and degradation pose significant threats to bird diversity worldwide. Baiyangdian, the largest freshwater lake wetland in North China, is an important habitat for birds. The degradation of water quality caused by decaying reed rhizomes has prompted governmental initiatives for ecological restoration in Baiyangdian. However, it has also led to the significant destruction of reed habitats within the wetlands consequently. Bird species that rely on these reed habitats, especially the reed parrotbill, face a significant threat, necessitating the establishment of species reserves to mitigate the loss of bird diversity. Our research aims to identify the potential suitable habitats for the reed parrotbill in Baiyangdian and establish priority conservation areas. Using the environmental factors determined with Google Earth Engine (GEE), ultimately we designated the following areas as priority conservation zones: the Fuhe Wetland (FHW), the reed area south of Beihezhuang (BHV), both sides of the Baiyangdian Bridge (BYDB), the western shoreline of Shaochedian (SCD), Yannandi Park (YNDP), east of Guangdianzhangzhuang Village (GDZZV), east of Dongtianzhuang (DTV), north of Xilizhuang (XLV), south of Caiputai Village (CPTV), north of Gaolou Village (GLV), and the Xiaoyihe Wetland (XYW). Our findings provide a scientific reference for ecological restoration projects in the Baiyangdian region and offer supporting data for the conservation management of the reed parrotbill. Full article
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15 pages, 3977 KB  
Article
Effects of Providing Enrichment to Broilers in an Animal Welfare Environment on Productivity, Litter Moisture, Gas Concentration (CO2 and NH3), Animal Welfare Indicators, and Stress Level Concentration
by Chan-Ho Kim, Woo-Do Lee, Ji-Seon Son, Jung-Hwan Jeon, Se-Jin Lim and Su-Mi Kim
Agriculture 2025, 15(2), 182; https://doi.org/10.3390/agriculture15020182 - 15 Jan 2025
Cited by 3 | Viewed by 4092
Abstract
As animal welfare becomes more active in livestock industry, research is being conducted on ways to improve poor housing environments, reduce stress, and meet welfare standards. Among these, environmental enrichment methods are effective in reducing stress and creating a welfare-friendly rearing environment, but [...] Read more.
As animal welfare becomes more active in livestock industry, research is being conducted on ways to improve poor housing environments, reduce stress, and meet welfare standards. Among these, environmental enrichment methods are effective in reducing stress and creating a welfare-friendly rearing environment, but there are few cases of actual application to farms. Therefore, we aimed to investigate the effect of providing pecking materials (grain blocks), known as one of the environmental enrichment methods, to commercial broiler farms. This study used two facilities that could accommodate 32,000 one-day-old broilers (Arbor acres) per building, and two groups (control and treatment groups) were designed after creating two identical areas within each building (total two treatments, two replicates, 16,000 birds per replicate). Two identical zones within the house were created by installing a partition in the center; one side was provided with grain blocks (one grain block per 1000 birds), and the other side was not. Analysis items included productivity (body weight, uniformity), environmental variables (litter and air), welfare indicators (leg, gait score, feather cleanliness score), and serum corticosterone levels. Analysis of all items was conducted twice, on the 19th and 27th, taking into account the farm’s feed change date and slaughter schedule. Other environmental conditions (density, lighting, ventilation, temperature, humidity, feed, and water) were the same. As a result, no difference in productivity was observed according to enrichment, and the quality of litter and air was similar. Also, there was no significant difference in welfare indicators. Interestingly, however, provision of the environment enrichment lowered serum corticosterone levels (p < 0.05). The implications of our study are that grain blocks as a pecking material are an effective way to reduce stress without adversely affecting broiler performance and rearing environment. However, it is still necessary to explore optimal enrichment materials that can help not only the welfare level but also the broiler performance. Full article
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11 pages, 5664 KB  
Proceeding Paper
Optimum Selection Analysis of Composite Cap Cone
by Jijun Liu, Yongping Jiang, Kaixiang Li and Yafeng Wang
Eng. Proc. 2024, 80(1), 10; https://doi.org/10.3390/engproc2024080010 - 3 Jan 2025
Cited by 1 | Viewed by 901
Abstract
An in-depth analysis was conducted on the dynamic strength design optimization of carbon fiber composite cap cones in aircraft engines subjected to bird body impacts. Initially, the top, 1/4, 1/2, 3/4, and root positions of the cap cone’s generatrix were designated as the [...] Read more.
An in-depth analysis was conducted on the dynamic strength design optimization of carbon fiber composite cap cones in aircraft engines subjected to bird body impacts. Initially, the top, 1/4, 1/2, 3/4, and root positions of the cap cone’s generatrix were designated as the impact sites. The analysis of bird impacts revealed that the 1/2 position along the generatrix is the most hazardous impact location. Subsequently, considering the thickness of the composite material cap cone as a variable and accounting for its high-speed rotational state, a bird impact analysis was performed at the most critical impact location. Additionally, a comparative study on the bird impact performance of the composite material cap cone under rotating and non-rotating conditions was conducted. The study indicates that, under identical conditions, the cap cone in rotation experiences more severe damage than in a non-rotating state, necessitating a cone thickness of 7 mm or greater; Subsequently, a bolt strength analysis model was established to thoroughly examine the impact of varying cone side thicknesses on the load applied to connecting bolts, and to assess bolt strength. The findings suggest that excessive bolt loads can also constrain the optimization of the cap cone; hence, finding the optimal balance between bolt quantity and strength is essential in design. Lastly, the study discussed the weakening of local stiffness in the composite material cap cone post-impact, noting a 12% decrease in its elastic mode frequency and the emergence of asymmetric vibration modes. This phenomenon could potentially lead to dynamic unbalanced loads, thus necessitating further evaluation in the optimization process of the cap cone. Full article
(This article belongs to the Proceedings of 2nd International Conference on Green Aviation (ICGA 2024))
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15 pages, 2356 KB  
Article
A New Species of the Feather Mite Genus Grallolichus Gaud, 1960 (Acariformes: Pterolichidae): First Report of a Commensal Mite Specific to the Sungrebe (Heliornis fulica)
by Jacek Dabert
Animals 2024, 14(20), 3035; https://doi.org/10.3390/ani14203035 - 19 Oct 2024
Cited by 3 | Viewed by 4969
Abstract
Feather mites of finfoots (Heliornithidae), a small gruiform family, are poorly and partly erroneously recognized. Grallolichus heliornisi sp. n. (Astigmata: Pterolichidae) is here described from the sungrebe Heliornis fulica as the first representative of the genus commonly found on close relatives of finfoots, [...] Read more.
Feather mites of finfoots (Heliornithidae), a small gruiform family, are poorly and partly erroneously recognized. Grallolichus heliornisi sp. n. (Astigmata: Pterolichidae) is here described from the sungrebe Heliornis fulica as the first representative of the genus commonly found on close relatives of finfoots, Rallidae and Sarothuridae. This species belongs to the species group having ornamented dorsal shields and is morphologically most close to G. proctogamus inhabiting Eurasian coot (Fulica atra). Males of the new species differ from G. proctogamus mainly by the shape of opisthosomal lobes (triangular vs. rounded) and the aedeagus form (parallel sided vs. tapering distally). Females differ mainly by the shape of supranal concavity (open anteriorly vs. closed) and location of setae h1 in relation to supranal concavity (lateral vs. anterior). A key to known species of the genus Grallolichus is provided. The morphological analysis and descriptive characterization of this species, like much of the approximately 2500 feather mite species described to date, were based on mummified mite material preserved in 19th-century old museum bird specimens. These often-forgotten collections are the only source for the analysis of the acarofauna of many rare, unavailable wild or even extinct bird taxa. Full article
(This article belongs to the Section Wildlife)
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21 pages, 12585 KB  
Article
Reinforcement Learning-Based Turning Control of Asymmetric Swept-Wing Drone Soaring in an Updraft
by Yunxiang Cui, De Yan and Zhiqiang Wan
Drones 2024, 8(9), 498; https://doi.org/10.3390/drones8090498 - 18 Sep 2024
Cited by 1 | Viewed by 2139
Abstract
Soaring drones can use updrafts to reduce flight energy consumption like soaring birds. With control surfaces that are similar to those of soaring birds, the soaring drone achieves roll control through asymmetric sweepback of the wing on one side. This will result in [...] Read more.
Soaring drones can use updrafts to reduce flight energy consumption like soaring birds. With control surfaces that are similar to those of soaring birds, the soaring drone achieves roll control through asymmetric sweepback of the wing on one side. This will result in asymmetry of the drone. The moment of inertia and the inertial product will change with the sweepback of the wing, causing nonlinearity and coupling in its dynamics, which is difficult to solve through traditional research methods. In addition, unlike general control objectives, the objective of this study was to enable the soaring drone to follow the soaring strategy. The soaring strategy determines the horizontal direction of the drone based on the vertical wind situation without the need for active control of the vertical movement of the drone. In essence, it is a horizontal trajectory tracking task. Therefore, based on the layout and aerodynamic data of the soaring drone, reinforcement learning was adopted in this study to construct a six-degree-of-freedom dynamic model and a control flight training simulation environment for the soaring drone with asymmetric deformation control surfaces. We compared the impact of key factors such as different state spaces and reward functions on the training results. The turning control agent was obtained, and trajectory-tracking simulations were conducted. Full article
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16 pages, 7409 KB  
Article
Influence of Herringbone Grooves Inspired by Bird Feathers on Aerodynamics of Compressor Cascade under Different Reynolds Number Conditions
by Shaobing Han, Zhijie Yang, Jingjun Zhong and Yuying Yan
Aerospace 2024, 11(8), 626; https://doi.org/10.3390/aerospace11080626 - 31 Jul 2024
Cited by 5 | Viewed by 1752
Abstract
Nowadays, high aerodynamic load has made blade separation an issue for compact axial compressors under high-altitude low-Reynolds-number conditions. In this study, herringbone grooves inspired by bird feathers were applied to suppress the suction side separation and reduce loss. To study the effect of [...] Read more.
Nowadays, high aerodynamic load has made blade separation an issue for compact axial compressors under high-altitude low-Reynolds-number conditions. In this study, herringbone grooves inspired by bird feathers were applied to suppress the suction side separation and reduce loss. To study the effect of bio-inspired herringbone grooves on the aerodynamic performance of compressor cascades, a high subsonic compressor cascade was taken as the research object. Under the conditions of different Reynolds numbers, the effects of herringbone grooves of different depths on the flow separation were numerically studied. The research results show that at a high-Reynolds-number condition (Re = 5.6 × 105), the sawtooth-shaped wake induced by herringbone grooves increases the turbulent mixing loss near the suction surface, and the blade performance deteriorates. At a low-Reynolds-number condition (Re = 1.3 × 105), the span-wise secondary flow and micro-vortex structure induced by the herringbone grooves effectively suppress the laminar separation on the suction surface of the blade, and there is an optimal depth for the herringbone grooves that reduces the profile loss by 8.33% and increases the static pressure ratio by 0.55%. The selection principle of the optimal groove depth with the Re is discussed based on the research results under six low-Reynolds-number conditions. Full article
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29 pages, 10993 KB  
Article
Detection of Growth Stages of Chilli Plants in a Hydroponic Grower Using Machine Vision and YOLOv8 Deep Learning Algorithms
by Florian Schneider, Jonas Swiatek and Mohieddine Jelali
Sustainability 2024, 16(15), 6420; https://doi.org/10.3390/su16156420 - 26 Jul 2024
Cited by 10 | Viewed by 4921
Abstract
Vertical indoor farming (VIF) with hydroponics offers a promising perspective for sustainable food production. Intelligent control of VIF system components plays a key role in reducing operating costs and increasing crop yields. Modern machine vision (MV) systems use deep learning (DL) in combination [...] Read more.
Vertical indoor farming (VIF) with hydroponics offers a promising perspective for sustainable food production. Intelligent control of VIF system components plays a key role in reducing operating costs and increasing crop yields. Modern machine vision (MV) systems use deep learning (DL) in combination with camera systems for various tasks in agriculture, such as disease and nutrient deficiency detection, and flower and fruit identification and classification for pollination and harvesting. This study presents the applicability of MV technology with DL modelling to detect the growth stages of chilli plants using YOLOv8 networks. The influence of different bird’s-eye view and side view datasets and different YOLOv8 architectures was analysed. To generate the image data for training and testing the YOLO models, chilli plants were grown in a hydroponic environment and imaged throughout their life cycle using four camera systems. The growth stages were divided into growing, flowering, and fruiting classes. All the trained YOLOv8 models showed reliable identification of growth stages with high accuracy. The results indicate that models trained with data from both views show better generalisation. YOLO’s middle architecture achieved the best performance. Full article
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26 pages, 1057 KB  
Review
Advances in Laboratory Diagnosis of Coronavirus Infections in Cattle
by Shaun van den Hurk, Girija Regmi, Hemant K. Naikare and Binu T. Velayudhan
Pathogens 2024, 13(7), 524; https://doi.org/10.3390/pathogens13070524 - 21 Jun 2024
Cited by 7 | Viewed by 4293
Abstract
Coronaviruses cause infections in humans and diverse species of animals and birds with a global distribution. Bovine coronavirus (BCoV) produces predominantly two forms of disease in cattle: a respiratory form and a gastrointestinal form. All age groups of cattle are affected by the [...] Read more.
Coronaviruses cause infections in humans and diverse species of animals and birds with a global distribution. Bovine coronavirus (BCoV) produces predominantly two forms of disease in cattle: a respiratory form and a gastrointestinal form. All age groups of cattle are affected by the respiratory form of coronavirus, whereas the gastroenteric form causes neonatal diarrhea or calf scours in young cattle and winter dysentery in adult cattle. The tremendous impacts of bovine respiratory disease and the associated losses are well-documented and underscore the importance of this pathogen. Beyond this, studies have demonstrated significant impacts on milk production associated with outbreaks of winter dysentery, with up to a 30% decrease in milk yield. In North America, BCoV was identified for the first time in 1972, and it continues to be a significant economic concern for the cattle industry. A number of conventional and molecular diagnostic assays are available for the detection of BCoV from clinical samples. Conventional assays for BCoV detection include virus isolation, which is challenging from clinical samples, electron microscopy, fluorescent antibody assays, and various immunoassays. Molecular tests are mainly based on nucleic acid detection and predominantly include conventional and real-time polymerase chain reaction (PCR) assays. Isothermal amplification assays and genome sequencing have gained increased interest in recent years for the detection, characterization, and identification of BCoV. It is believed that isothermal amplification assays, such as loop-mediated isothermal amplification and recombinase polymerase amplification, among others, could aid the development of barn-side point-of-care tests for BCoV. The present study reviewed the literature on coronavirus infections in cattle from the last three and a half decades and presents information mainly on the current and advancing diagnostics in addition to epidemiology, clinical presentations, and the impact of the disease on the cattle industry. Full article
(This article belongs to the Special Issue Diagnostics of Emerging and Re-Emerging Pathogens)
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16 pages, 3886 KB  
Article
Performance Comparison of Five Methods Available in ImageJ for Bird Counting and Detection from Video Datasets
by Kevin Adi Kurnia, Ferry Saputra, Cao Thang Luong, Marri Jmelou M. Roldan, Tai-Sheng Cheng and Chung-Der Hsiao
Inventions 2024, 9(3), 55; https://doi.org/10.3390/inventions9030055 - 9 May 2024
Cited by 1 | Viewed by 4391
Abstract
Bird monitoring is an important approach to studying the diversity and abundance of birds, especially during migration, as it can provide core data for bird conservation purposes. The previous methods for bird number estimation are largely based on manual counting, which suffers from [...] Read more.
Bird monitoring is an important approach to studying the diversity and abundance of birds, especially during migration, as it can provide core data for bird conservation purposes. The previous methods for bird number estimation are largely based on manual counting, which suffers from low throughput and a high error rate. In this study, we aimed to provide an alternative bird-counting method from video datasets by using five available ImageJ methods: Particle Analyzer, Find Maxima, Watershed segmentation, TrackMate, and trainable WEKA segmentation. The numbers of birds and their XY coordinates were extracted from videos to conduct a side-by-side comparison with the manual counting results, and the three important criteria of the sensitivity, precision, and F1 score were calculated for the performance evaluation. From the tests, which we conducted for four different cases with different bird numbers or flying patterns, TrackMate had the best overall performance for counting birds and pinpointing their locations, followed by Particle Analyzer, Find Maxima, WEKA, and lastly, Watershed, which showed low precision in most of the cases. In summary, five ImageJ-based counting methods were compared in this study, and we validated that TrackMate obtains the best performance for bird counting and detection. Full article
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18 pages, 3856 KB  
Article
A New Double-Step Process of Shortening Fibers without Change in Molding Equipment Followed by Electron Beam to Strengthen Short Glass Fiber Reinforced Polyester BMC
by Michael C. Faudree and Yoshitake Nishi
Materials 2024, 17(9), 2036; https://doi.org/10.3390/ma17092036 - 26 Apr 2024
Cited by 1 | Viewed by 1805
Abstract
It is vital to maximize the safety of outdoor constructions, airplanes, and space vehicles by protecting against the impact of airborne debris from increasing winds due to climate change, or from bird strikes or micrometeoroids. In a widely-used compression-molded short glass fiber polyester [...] Read more.
It is vital to maximize the safety of outdoor constructions, airplanes, and space vehicles by protecting against the impact of airborne debris from increasing winds due to climate change, or from bird strikes or micrometeoroids. In a widely-used compression-molded short glass fiber polyester bulk-molded compound (SGFRP-BMC) with 55% wt. CaCO3 filler, the center of the mother panel has lower impact strength than the outer sections with solidification texture angles and short glass fiber (SGF) orientations being random from 0 to 90 degrees. Therefore, a new double-step process of: (1) reducing commercial fiber length without change in molding equipment; followed by a (2) 0.86 MGy dose of homogeneous low-voltage electron beam irradiation (HLEBI) to both sides of the finished samples requiring no chemicals or additives, which is shown to increase the Charpy impact value (auc) about 50% from 6.26 to 9.59 kJm−2 at median-accumulative probability of fracture, Pf = 0.500. Shortening the SGFs results in higher fiber spacing density, Sf, as the thermal compressive stress site proliferation by action of the CTE difference between the matrix and SGF while the composite cools and shrinks. To boost impact strength further, HLEBI provides additional nano-compressive stresses by generating dangling bonds (DBs) creating repulsive forces while increasing SGF/matrix adhesion. Increased internal cracking apparently occurs, raising the auc. Full article
(This article belongs to the Special Issue Recent Researches in Polymer and Plastic Processing)
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