Search Results (220)

Nanofiber scaffolds play a crucial role in bioengineering by providing structural support for tissue and organoid growth. For composite nanofibers, optimizing their properties for specific applications often requires analyzing the spatial distribution of their chemical structure. This review focuses on the applications of Raman hyperspectral imaging to the mapping of the chemical composition of nanofibers. While the technique is diffraction-limited to the size of the scanning beam, it is possible to decipher the nanoscale features of these fibers by employing oversampling during scanning. Subsequently, these oversampled data can be analyzed by a singular-value decomposition (SVD) analysis and classical least-squares (CLS) decomposition. In many cases, this technique is essential for verifying the spatial distribution of different chemical components within multi-component nanofibers. Full article

Ovarian tissue cryopreservation (OTC) has emerged as the only viable fertility preservation strategy for prepubertal girls and adolescent cancer patients facing gonadotoxic treatments. While OTC has transitioned from an experimental procedure to an established clinical practice, the functional longevity of transplanted grafts remains limited by massive follicle depletion. This review synthesizes recent technological advances in OTC for female children, with a particular focus on the underlying molecular mechanisms and innovative protective strategies. We systematically evaluate pre-cryopreservation assessments, surgical harvesting techniques such as medulla-sparing biopsies, and the comparative efficacy of slow freezing versus vitrification in preserving stromal and follicular integrity. Central to this discussion are the molecular drivers of post-transplantation injury, including ischemia–reperfusion-induced oxidative stress and the iatrogenic over-activation of the PI3K/Akt/mTOR signaling pathway, which leads to follicular “burnout.” Furthermore, we explore targeted pharmacological interventions, such as the dual-drug application of VEGFA and rapamycin, alongside emerging bioengineering frontiers including decellularized extracellular matrix scaffolds and 3D-printed bioprosthetic ovaries. Clinical outcomes are also summarized, highlighting high rates of endocrine recovery (~95%) and promising live birth rates (~28%), predominantly through natural conception. By integrating deep molecular insights with advanced tissue engineering, this review provides a comprehensive framework for optimizing long-term fertility restoration and improving the quality of survivorship for young female cancer survivors. Full article

Background: Nasopharyngeal carcinoma (NPC) presents significant diagnostic and therapeutic challenges, often due to late-stage detection and its complex anatomical location. The increasing integration of artificial intelligence (AI) into oncology offers potential opportunities to enhance the precision of NPC management. This systematic review aims to synthesise the current evidence of AI applications in NPC diagnosis, prognostication, and treatment planning. Methods: A systematic literature search was conducted following PRISMA guidelines across multiple databases (PubMed, Scopus, Embase, Google Scholar, IEEE Xplore) for studies published up to June 2025. From an initial pool of 2549 articles, 55 studies meeting the inclusion criteria were selected for qualitative analysis. The review focuses on AI models applied to key diagnostic modalities: computed tomography (CT), magnetic resonance imaging (MRI), and histopathological whole-slide images (WSI). Results: AI, particularly deep learning (DL), shows promising performance in automating critical tasks across all modalities. For CT and MRI, models have been reported to achieve accurate tumor and organ-at-risk segmentation, potentially supporting radiotherapy planning, and show strong performance in predicting survival outcomes and treatment toxicity. In digital pathology, AI enables automated diagnosis and facilitates the extraction of prognostic “pathomic” features from WSIs, with some studies suggesting performance comparable to or exceeding traditional radiomics. The most significant advances are seen in multimodal AI systems that integrate radiological, pathological, and clinical data, which, in some studies, show modest improvements in prognostic performance compared to single-modality approaches. However, these findings are preliminary, as none of the reviewed multimodal models underwent rigorous external validation in large, multi-center cohorts. Reported performance varies considerably across studies, and claims of superiority should be interpreted with caution. Full article

Background: Deep burns in pediatric population often require split-thickness skin grafts (STSGs) and the identification of an optimal donor site is crucial to minimize morbidity, accelerate healing and reduce short- and long-term complications. The scalp appears to be increasingly used in clinical practice, but evidence remains limited, despite the promise of novel bioengineering and regenerative approaches. Methods: A systematic review about the use of scalp for STSG in pediatrics was conducted across PubMed, Scopus, and Cochrane (2005–2025). Clinical outcomes considered were donor-site healing time, early and late complications, perioperative practices, and quality of scars. Results: Four studies met the inclusion criteria (n = 417, mean age 2.9–7.3 years) with follow-up periods up to 27 years. Epithelialization occurred between 7 and 25 days. Early complications included folliculitis (up to 44% in certain hair types) and delayed healing (n = 13; 52%). A rigorous harvesting technique is needed to avoid preventable complications. Late sequelae included alopecia (1.6% to 33%—the latter largely unperceived by patients) and hypertrophic scarring (1.6–4%). Scar quality was rated good in >80% of cases. Conclusions: Evidence supports the scalp as a safe, efficient, and cosmetically favorable donor site for pediatric STSG. Based on evidence and clinical experience, we propose the first structured scalp-donor management algorithm to optimize safety, reduce complications, and standardize perioperative care in the management of pediatric burns. Full article

Pulmonary bioengineering holds significant promise for the development of functional lungs suitable for transplantation in patients with terminal lung diseases; however, it encounters considerable challenges. The inherent structural complexity, diverse cellular composition, and the intricate process of re-endothelialization the pulmonary vasculature complicate efforts to reconstruct viable lungs for transplantation. This study aimed to establish an innovative re-endothelialization technique utilizing decellularized scaffolds, integrating canine yolk sac-derived endothelial precursor cells with mechanical respiratory stimuli within a bioreactor framework. Wistar rat lungs were subjected to a decellularization protocol employing SDS + Triton X-100 0.5% and subsequently assessed for cytocompatibility with murine fibroblasts (3T3) and yolk sac (YS) cells in fragments. Following this, the recellularization of the whole-lung scaffold was evaluated under constant mechanical respiratory stimulation with YS cells. Each stage of the process was rigorously analyzed using histological staining, DAPI, scanning electron microscopy (SEM), and genomic DNA quantification. The findings reveal that the implemented alternating decellularization protocol resulted in a structured scaffold conducive to the culture of various cell types in fragments. When subjected to the complete scaffold recellularization model, the results indicated that YS cells are advantageous for the re-endothelialization process. Moreover, when employed in conjunction with the bioreactor model incorporating respiratory stimulation, these cells demonstrated enhanced cellular diffusion capacity and facilitated more homogeneous recellularization of the entire organ. These results signify a notable advancement in the reconstruction of new tissues for pulmonary transplantation. Full article

Background: Coronary artery bypass grafting is the optimal revascularization strategy for patients with complex multivessel coronary artery disease. However, saphenous vein grafts are associated with high failure rates and donor site morbidity. Off-the-shelf tissue-engineered vascular grafts offer a potential solution for patients lacking suitable autologous vessels. Here, we report the first successful clinical implant of an acellular Tissue-Engineered Vessel (TEV) for coronary artery bypass grafting in Europe. Methods: A 73-year-old male with two-vessel disease and no suitable autologous vein underwent on-pump coronary artery bypass grafting using the left internal mammary artery to the left anterior descending artery and a 4 mm TEV to the right coronary artery. Results: Implant procedure followed standard surgical techniques, sutures and duration. The conduit handling was comparable to native vessels. Intraoperative flow measurements demonstrated excellent graft performance (TEV: 110 mL/min, Pulsatility Index 1.0). Postoperative recovery was uneventful. One-month computed tomography coronary angiography confirmed graft patency. Discussion: This case demonstrates the feasibility of using a bioengineered conduit for coronary revascularization in patients without suitable autologous grafts. If these findings are confirmed in larger trials, bioengineered vessels could expand surgical revascularization to patients without suitable autologous conduits and fundamentally alter conduit selection strategy in CABG. Conclusions: This first-in-Europe clinical implant demonstrates that an off-the-shelf acellular tissue-engineered vessel can meet the procedural, hemodynamics, and patency requirements of coronary artery bypass. These proof-of-concept results support progression to prospective multi-center evaluation. Full article

Traumatic injury, stenosis, and malignancy involving large segments of the airway are difficult to reconstruct and require novel solutions. Despite advances in surgical techniques, the reconstruction of long-segment tracheal defects remains a significant challenge. Several bioengineering approaches have been explored for tracheal regeneration in vitro and in vivo, using cells in combination with three dimentional (3D) biological or synthetic scaffolds. This paper reviews recent advances in developing bioengineered trachea and the technologies utilized toward generating transplantable tracheal grafts. Specifically, the review will focus on the recellularization of tissue-engineered grafts using natural or synthetic scaffolds, highlighting relevant cell types used to reconstitute tracheal epithelium and cartilage. The promise of newly explored paradigms, including the application of pluripotent stem cells, will be discussed with an overview of associated challenges and necessary steps for future translation. Overall, these advances provide a foundation for the development of clinically viable tracheal grafts, bringing engineered tracheal reconstruction closer to reality. Full article

Objective: Bilateral cleft lip and palate (BCLP) is a craniofacial malformation often associated with anatomical and functional impairments, including a shallow oral vestibule in the premaxillary region. This condition requires a vestibuloplasty to restore the space between the teeth and upper lip, also improving aesthetics. Traditional techniques frequently require autologous grafts, leading to increased morbidity. Tissue bioengineering provides suitable alternatives. Methods: We present the case of a 19-year-old female, who had undergone several surgeries for BCLP management, complaining of a lack of upper lip projection and an insufficient vestibular dept in the premaxilla region. We reported short-term follow-up using Acellular Fish Skin Graft (AFSG, Kerecis™) as a substitute to autologous graft to perform a vestibuloplasty. Outcome assessment was based on clinical measurements. Results: The graft showed early signs of vascularization. Clinical outcomes included improved vestibular depth, from 1 mm preoperatively to 8 mm, upper lip projection, and functional mobility after a six-month follow-up period. The patient experienced an uneventful recovery and was satisfied with the aesthetic result. Conclusions: This case, presenting the first application of AFSG in vestibuloplasty, tends to reassure us about its safety. AFSG might be an effective biocompatible alternative to autologous grafts in vestibuloplasty, offering promising results in mucogingival reconstruction. Further studies are needed to confirm long-term stability and integration in oral tissues. Full article

Steviol glycosides (SGs) are high-intensity, zero-calorie natural sweeteners with demonstrated safety and potential health benefits, positioning them as ideal sucrose substitutes for metabolic disorder management. However, their broad application is limited by inherent drawbacks such as bitterness, low solubility, and inefficient production systems. This review provides a comprehensive summary of recent advances in SG research, covering their sources, properties, and bioactivities. A particular focus is placed on innovative bioproduction strategies—including enzyme engineering, metabolic pathway optimization, and sustainable extraction techniques. Strategies to overcome these challenges through sensory-function enhancement—including formulation and structural modification—are discussed. Furthermore, it highlights emerging trends like microbial chassis-based production and next-generation sweetener design, providing actionable insights for overcoming industrial bottlenecks. By integrating multidisciplinary advances in bioengineering, sensory science, and sustainable processing, this review offers a forward-looking perspective on the development and application of SGs as functional sweeteners in the global food industry. Full article

Spina bifida (SB) is a congenital malformation of the central nervous system (CNS), resulting from incomplete closure of the neural tube (NT) during early embryogenesis. Myelomeningocele (MMC), the most severe form of SB, leads to progressive neurological, orthopedic, and urological dysfunctions due to both NT developmental failure and secondary intrauterine injury (“two-hit hypothesis”). Prenatal repair of MMC has progressed considerably since the Management of Myelomeningocele Study (MOMS, 2011) trial, which showed that open fetal surgery can decrease the need for shunting and improve motor function, although it carries significant maternal risks. To address these limitations, minimally invasive techniques have been developed, with the goal of achieving similar benefits for the fetus while reducing maternal morbidity. Recent research has shifted toward regenerative strategies, integrating mesenchymal stem cells (MSCs), bioengineered scaffolds, and cell-derived products to move beyond mere mechanical protection toward true NT repair. Preclinical studies in rodent and ovine models have shown that amniotic- and placenta-derived MSCs exert neuroprotective and immunomodulatory paracrine effects, promoting angiogenesis, modulating inflammation, and supporting tissue regeneration. Minimally invasive, cell-based interventions such as Transamniotic Stem Cell Therapy (TRASCET), in preclinical rodent models, offer the possibility of very early treatment without hysterotomy, although translation remains limited by the lack of large-animal validation and long-term safety data. In parallel, advances in biomaterials, nanostructured scaffolds, and exosome-based therapies reinforce a regenerative paradigm that may improve neurological outcomes and quality of life in affected children. Ongoing translational studies are essential to optimize these approaches and define their safety and efficacy in clinical settings. This review provides an integrated overview of embryological mechanisms, diagnostic strategies, and prenatal therapeutic advances in SB treatment, with emphasis on prenatal repair, fetal surgery and emerging regenerative approaches. Full article

Tissue regeneration is essential for wound healing, organ function restoration, and overall patient recovery. Its success significantly impacts medical procedures in fields like internal medicine and orthopedics, enhancing patient quality of life. Recent advances in regenerative medicine, particularly the combination of advanced drug delivery systems (DDS) and bioengineering, have enabled customized methods to improve tissue regeneration outcomes. However, conventional tissue engineering techniques have drawbacks, often using static scaffolds that lack the dynamic properties of real tissues, leading to subpar healing outcomes. The use of 3D printing and other advanced scaffolding techniques allows for the creation of bio functional scaffolds that deliver bioactive molecules at precise locations and times. The optimal integration of biological systems with enhanced material properties for personalized treatment options remains unclear. There is a need for more research into the complex interactions between cellular biology, drug delivery, and material technology to improve tissue regeneration. Despite progress in developing bioactive scaffolds and localized drug delivery methods, the interactions among different scaffold materials, bioactive agents, and cellular behaviors within the regenerative ecosystem are not fully understood. While there is extensive research on 3D-printed scaffolds in tissue engineering, there is a lack of studies integrating bio printing with in vivo biological reactions in real time. Limited research on the dynamic integration of patient-specific parameters in regeneration methods highlights the need for customized approaches that consider individual physiological differences and the complex biological environment at injury sites. Additionally, challenges arise when translating laboratory results into effective therapeutic applications, underscoring the necessity for interdisciplinary collaboration and innovative design approaches that align advanced material properties with biological needs. Full article

With the growing emphasis on bio-engineering techniques, the sustainable advantages of using trees as barriers against rockfalls have become increasingly evident. The key mechanism for forest protection against rockfalls is the dissipation of block kinetic energy during impacts. However, previous studies have primarily focused on the overall attributes of protection forests, with limited attention to the quantitative relationship between internal spatial structural parameters and protective effectiveness. This study systematically investigated the effects of tree diameter, plant spacing, and arrangement pattern on rockfall energy dissipation through physical experiments. The results indicate that: (1) The energy dissipation capacity of trees increases with tree diameter; however, the rate of increase declines significantly when the relative diameter (the ratio of tree diameter to block size) exceeds 0.4. (2) Rockfall energy dissipation increases with reduced plant spacing, but the resultant gain exhibits a diminishing trend. (3) Under otherwise identical conditions, the rhombus arrangement pattern achieved a significantly higher rockfall energy dissipation rate (82.67%) than the square pattern (49.28%). Based on the experimental findings, an optimized protection scheme was designed for a typical rockfall on the slope of the Lehong Tunnel in Yunnan Province, southwestern China. Three-dimensional numerical simulation validated the designed scheme. The designed protection forests dissipated 89.49% of the kinetic energy from 0.5 m blocks, demonstrating the practical efficacy of the parameters derived from experiments. This study quantifies the influence of internal spatial structure parameters on the protective effectiveness of forests against rockfalls, providing a valuable theoretical basis and practical guidance for the design of ecological prevention measures against rockfall hazards. Full article

Plant growth regulators (PGRs) are natural or synthetic substances that control and manipulate plant physiological processes, controlling branching and vegetative growth. Maintaining roadside vegetation through frequent mowing is costly, dangerous, and unsustainable. This narrative literature review proposes a revolution in this management by conducting a systematic literature review on the strategic application of PGRs on roadsides. Practices such as the application of plant growth regulators, the use of native cover crops, and bioengineering techniques with stabilizing species were analyzed. Previous studies have shown that the use of regulators such as mepiquat chloride and paclobutrazol reduces plant height and aboveground biomass, favoring growth control and compacting the plant architecture. The environmental and operational impacts related to vegetation control on roadside strips were also considered. Integrated with LiDAR technology for precise monitoring, this model establishes a new paradigm: smart, safe, and sustainable. Therefore, it is hoped that this compendium will fill a gap in national guidelines by offering an evidence-based protocol guideline for the use of PGR as an alternative to traditional management methods, thus reducing the number of mowing and weeding operations in highway right-of-way areas. Full article

The critical shortage of donor organs remains the foremost challenge in transplantation medicine. Nevertheless, advancements in robotic-assisted surgery (RAS), artificial intelligence (AI)-enhanced donor–recipient matching, and bioengineering—particularly 3D bioprinting—are revolutionizing the field. Today, RAS has evolved from an innovative technique into a reliable clinical tool, with evidence indicating that it enhances surgical precision and results in better patient outcomes. Meanwhile, AI and machine learning are advancing donor–recipient matching and allocation, producing models that offer superior predictive accuracy for graft survival compared to traditional methods. Additionally, bioengineering strategies, especially 3D bioprinting and tissue engineering, are progressing from the creation of acellular scaffolds to the development of vascularized constructs, marking a significant milestone toward functional organ replacement. Despite persistent challenges such as high costs, regulatory obstacles, new structured formation programs, and the necessity for effective vascularization in engineered tissues, the integration of these disciplines is forging a new paradigm in regenerative medicine. The primary objective of this review is to synthesize multidisciplinary innovations by leveraging clinical studies and technological assessments to delineate future directions in regenerative medicine and organ transplantation. Full article

Antimicrobial peptides (AMPs), evolutionarily conserved components of the immune system, have attracted considerable attention as promising therapeutic candidates. Derived from diverse organisms, AMPs represent a heterogeneous class of molecules, typically cationic, which facilitates their initial electrostatic interaction with anionic microbial membranes. Unlike conventional single-target antibiotics, AMPs utilize rapid, multi-target mechanisms, primarily physical membrane disruption, which results in a significantly lower incidence of resistance emergence. Their broad-spectrum antimicrobial activity, capacity to modulate host immunity, and unique mechanisms of action make them inherently less susceptible to resistance compared with traditional antibiotics. Despite these advantages, the clinical translation of natural AMPs remains limited by several challenges, including poor in vivo stability, and potential cytotoxicity. Bioengineering technology offers innovative solutions to these limitations of AMPs. Two techniques have demonstrated promise: (i) a chimeric recombinant of AMPs with stable scaffold, such as human serum albumin and antibody Fc domain and (ii) chemical modification approaches, such as lipidation. This review provides a comprehensive overview of AMPs, highlighting their origins, structures, and mechanisms of antimicrobial activity, followed by recent advances in bioengineering platforms designed to overcome their therapeutic limitations. By integrating natural AMPs with bioengineering and nanotechnologies, AMPs may be developed into next-generation antibiotics. Full article