Search Results (2,688)

The development of new methods enhancing the growth and yield of cereals is still needed in crop production due to their great importance in human diet and as livestock fodder. In our study, new fertilizer-biostimulators with micro- and macroelements and extract containing lipochitooligosaccharides (LCOs) produced by Rhizobium leguminosarum bv. trifolii were used for stimulation of growth of barley and triticale in greenhouse conditions. The preparations were applied at the tillering and shooting stages, whereas plant traits were studied at flowering and at full maturity. The best results were recorded after the joint treatment of the plants with LCOs and mineral fertilization. The application of such a mixture significantly increased the length and mass of roots at flowering in both studied species. A beneficial effect of the treatment was also observed in barley and triticale yields. At full maturity, the grain mass per plant was significantly enhanced, which was the effect of an increased number of grains per ear and increased mass of 1000 grains. Full article

A three-year experiment was conducted over the years 2020–2022 to determine the spectrum of microscopic fungi colonizing the grain of two fungicide-treated cultivars of spring barley and the profiles of mycotoxins identified in grain. In comparison with the unprotected control, fungicide treatment significantly increased grain yield by an average of approximately 10% in cv. Atrika and approximately 20% in cv. Vermont. The most abundantly isolated species were Alternaria alternata and Bipolaris sorokiniana. Fungi of the genus Fusarium were also widely represented, accounting for 7% to 27% of all isolates, depending on the year. Each year, 45 secondary fungal metabolites produced mainly by Fusarium and Alternaria species were identified. Fungicide protection did not reduce the overall concentration of Fusarium toxins and even caused a slight increase, while contributing to a decrease in the levels of nivalenol-3-glucoside, nivalenol, and deoxynivalenol. Concurrently, the concentrations of group A trichothecenes and moniliformin increased. The grain of spring barley cv. Vermont contained higher levels of the major Fusarium toxins than the grain of cv. Atrika. Non-parametric Friedman ANOVA revealed significant differences between years for eight mycotoxin concentrations. These results confirm the complex effects of chemical protection on the composition of grain microflora and mycotoxin profiles, indicating the need for further research into interactions between cultivars, environmental conditions, and integrated plant protection strategies in the production of food and feed cereals to improve food safety. Full article

Growers in the grain-producing continental cold desert and cold semiarid regions are interested in the local adaptation of winter malting barley (Hordeum vulgare) as a potential alternative crop to winter wheat (Triticum aestivum). Variety selection for specific environments is a critical first step in producing high yields of winter malting barley at the same production costs. Twenty-two winter malting barley entries were planted under irrigation in randomized complete blocks at New Mexico State University’s Agricultural Science Center at Farmington (cold desert; 3 replicates) and Rex E. Kirksey Agricultural Science Center at Tucumcari (cold semiarid; 4 replicates) in September 2023 and harvested for grain in July 2024. All entries at Tucumcari were heavily grazed by wildlife over winter, which may have influenced grain production of some varieties, although there was no site × cultivar interaction for grain yield, which ranged from 2558 to 4157 kg ha⁻¹. Irrigation and N fertilization differences between sites likely influenced (p < 0.0001) grain yield and grain protein (4421 and 2172 kg grain yield ha⁻¹ at Farmington and Tucumcari, respectively; 109 and 93 g grain protein kg⁻¹ at Farmington and Tucumcari, respectively). Future research in cold desert and semiarid regions should evaluate cultivar differences regarding irrigation and nutrient management. Full article

Accurate crop classification is critical for optimizing agricultural resource use and informing production decisions. Deep learning, with its robust feature extraction ability, has become a prevalent technique for remote sensing-based crop classification. However, agricultural landscape complexity poses three key challenges: background noise interference, class confusion from inter-crop spectral similarity, and blurred small-area crop boundaries due to class imbalance. This paper proposes FCR-TransUNet, a TransUNet-based enhanced model integrating three modules: Feature Enhancement Module (FEM) for noise filtering, Class-Attention (CAExperimental results on the Youyi Farm and barley datasets validate the superiority of the proposed model. On the Youyi Farm dataset, FCR-TransUNet achieves an MIoU of 92.2%, representing an improvement of 1.8% over SAM2-UNet and 2.9% over the baseline TransUNet. On the barley dataset, it yields an MIoU of 89.9%. Ablation studies further verify the effectiveness of each designed module. To comprehensively evaluate the classification performance of FCR-TransUNet across the full crop growth cycle, experiments were conducted using remote sensing images from May, July, and August, respectively. The results demonstrate that FCR-TransUNet exhibits strong stability and adaptability at different crop growth stages, providing a reliable solution for precision agriculture and intelligent agricultural production. Full article

Driven by the growing demand for flavor diversification in the global craft beer market, conventional drum roasting for specialty malt faces limitations in time consumption and flavor retention. This study aimed to explore explosive puffing as a novel approach for specialty malt production. Base barley malt was treated via explosive puffing at 0.8 MPa to prepare puffed specialty malt, followed by comprehensive characterization of its physicochemical properties, volatile profile, and antioxidant activity, with brewing trials conducted at 15% grist substitution. Results showed that puffed malt reached a color of 183.15 EBC, with formation of roasted pyrazines and caramel-like furans, and a nearly 3-fold increase in total phenolic content and antioxidant capacity. At 15% addition, the puffed malt maintained wort free amino nitrogen and reducing sugar levels, while significantly enhancing beer color, roasted aroma, and antioxidant activity. These findings demonstrate that explosive puffing is a promising alternative to conventional roasting for producing specialty malt. Full article

Managing tillage intensity and diversifying crop rotation are important sustainability levers for conservation agriculture (CA) with the potential to enhance crop resilience, resource efficiency, and yield stability. Accordingly, this study aimed to determine the effect of reduced tillage intensities and cereal–legume rotation systems on the agronomic and physiological performance of rainfed durum wheat grown under Mediterranean semi-arid conditions. To this end, a two cropping seasons field experiment was conducted in northeast Tunisia where the combined effects of two reduced tillage intensities (minimum and no-tillage; MT and NT) and two legume-based crop rotation systems (biennial and triennial; B and T) were compared to the more traditional conventionally tilled monocropping system (CT and M). Crop rotation, particularly when integrated with no-tillage (NT), significantly improved wheat development and grain yield, along with key yield attributes such as thousand-kernel weight and spike density. The interaction between tillage and crop sequence was highly influential; for instance, the NT × T (no-tillage × triennial rotation) combination achieved the highest grain yields (240 and 236 g m⁻² in 2020–2021 and 2021–2022, respectively), while the CT × M (conventional tillage × monoculture) interaction resulted in the lowest productivity (143 and 135 g m⁻²). Physiologically, the integration of reduced tillage and legume–cereal rotations optimized the photosynthetic apparatus, as evidenced by significantly improved chlorophyll fluorescence parameters. However, a prominent trade-off was identified: while NT × T maximized productivity, conventional tillage (CT) maintained superior grain protein (18.6%) and gluten concentrations, indicating a nitrogen dilution effect in high-yielding conservation systems. These results demonstrate that while no-tillage and triennial rotations (faba bean–wheat–barley) are robust strategies for climate-resilient yields in semi-arid environments, they must be coupled with optimized nitrogen management to offset quality declines. Consequently, this study establishes the NT × T interaction as a superior model for sustainable rainfed farming, provided that nutrient synchronization is addressed to ensure nutritional security under increasingly unpredictable Mediterranean climates. Full article

Barley remains the fourth most cultivated cereal crop worldwide and is valued for its versatility in malting and brewing, animal feed, human nutrition, and dietary supplements. The identification of genotypes suitable for breeding or specific end-use applications requires multi-environment testing to evaluate agronomic performance, grain quality, and trait stability. In this study, a panel of 50 winter barley genotypes (two-row and six-row) originating from diverse genetic backgrounds was evaluated over three growing seasons (2021–2023) under the environmental conditions of southeastern Romania. Seven traits were analyzed, including three phenological traits (heading time, flowering time and plant height), grain yield, and three quality parameters (thousand-grain weight, protein content, and starch content). Environmental conditions had a strong influence on phenological development and grain yield, whereas grain quality traits showed relatively greater stability, indicating a stronger genetic control. Multivariate analyses (Principal Component Analysis (PCA) and Genotype plus Genotype-by-Environment interaction biplot (GGE biplots)) revealed clear relationships among traits and highlighted contrasting adaptive strategies between the two barley types. In two-row barley, genotypes such as Idra and Sandra combined favorable yield performance with stable grain quality traits and therefore represent promising candidates for breeding programs and large-scale cultivation. In six-row barley, SU-Ellen and LG Zebra showed high productivity and strong starch accumulation, making them valuable genetic resources for yield-oriented breeding, although further improvement in nitrogen use efficiency may be beneficial. The 2022–2023 growing season represented the most restrictive environment, emphasizing the importance of stability under stress conditions. Genotypes located close to the Average Environment Coordination axis (AEC axis) during that season, such as Ametist (six-row) and Lardeya (two-row), may represent promising material for breeding programs targeting drought resilience. Overall, the results expand the phenotypic characterization of winter barley germplasm and identify valuable genetic resources that can support pre-breeding efforts and the development of climate-resilient barley cultivars. Full article

This study used highland barley straw from the Tibetan Plateau to cultivate Pleurotus ostreatus, Pholiota nameko, Lentinula edodes, Pleurotus eryngii, and Hericium erinaceus, addressing straw waste, forage shortages, and underutilized barley straw. The results showed that highland barley straw was suitable for cultivating P. ostreatus and P. nameko, with P. ostreatus yielding significantly more. After fruiting, spent mushroom substrates (SMS) from both species had higher crude protein, fat, and ash, with reduced fiber content compared to raw straw. P. ostreatus SMS showed greater protein accumulation and fiber degradation, offering better feed quality than P. nameko. Fungal communities were more concentrated under P. ostreatus, while P. nameko had higher diversity. Multivariate analyses showed that fungal community structure correlated with protein, fat, and feed quality, while bacterial communities were linked to fiber content. Functional predictions indicated that P. ostreatus enriched carbohydrate and energy metabolism pathways, while P. nameko was more associated with biosynthetic functions. Overall, cultivating mushrooms on barley straw improved SMS feed quality, with P. ostreatus showing greater potential for feed use. Full article

This study investigated the effects of isoacid supplementation on in vitro rumen fermentation characteristics, nutrient degradability, and bacterial community diversity in yaks using corn silage–highland barley straw-based substrates. An in vitro fermentation experiment was conducted with a substrate consisting of 80% whole-plant corn silage and 20% highland barley straw. Treatments included a control (without isoacids) and four isoacid supplemental levels (0.1%, 0.2%, 0.3%, and 0.4% of substrate dry matter, DM), each with six replicates. A 72 h in vitro gas production experiment was performed to measure cumulative gas production, fermentation parameters, nutrient degradability, and bacterial community diversity. Cumulative gas production increased by 12.96% with 0.2% isoacid supplementation compared to the control (p < 0.05). The contents of microbial protein (MCP), acetate, propionate, and total volatile fatty acids (TVFA) exhibited quadratic responses to the increasing isoacid dosage (p < 0.05). Specifically, MCP content reached a maximum of 0.76 mg/mL with 0.2% isoacids, representing a 31.03% increase compared to the control (p < 0.05). TVFA content was highest (146.85 mmoL/L) at 0.2% isoacid supplementation, with a 16.40% increase compared to the control (p < 0.05). Acetate content increased by 17.99% (p < 0.05), while propionate tended to increase with 0.2% isoacid supplementation (p = 0.08). Supplementation with 0.2% and 0.4% isoacids did not alter the bacterial composition and diversity (p > 0.05). However, at the genus level, g_Ruminococcus, g__Elusimicrobium, g_norank_f_Atopobiaceae, g_norank_o_Coriobacteriales, and g_Romboutsia were identified as differential biomarkers showing significant responses to isoacid supplementation (p < 0.05). Mantel-test analysis revealed positive correlation between g_Ruminococcus abundance and NH₃-N content (r < 0.4, p < 0.05); g_Romboutsia abundance and acetate content (r < 0.40, p < 0.05); g_Defluviitaleaceae_UCG-011 abundance and both NH₃-N content and the pH of rumen fluid (r < 0.40, p < 0.05); g_norank_o_Coriobacteriales abundance and rumen pH (r < 0.40, p < 0.01). Supplementation with 0.2% isoacids to corn silage–barley straw substrates improved in vitro rumen fermentation characteristics in yaks, which was associated with altered abundances of key bacterial genera including g_Ruminococcus, g__Elusimicrobium, g_norank_f_Atopobiaceae, g_norank_o_Coriobacteriales. Full article

Winter survival in barley depends on freezing tolerance and de-acclimation tolerance, yet their genetic determinants under increasingly unstable winters remain poorly understood. Here, 188 Polish barley accessions were evaluated over two consecutive growing seasons (2021–2022) using genome-wide association studies (GWAS) with a mixed-linear model (MLM) and high-density single nucleotide polymorphism (SNP) and diversity arrays technology sequencing (DArTseq) markers. Freezing and de-acclimation tolerance were quantified by 16 chlorophyll fluorescence parameters and post-freezing survival rates in plants subjected to 21 days of cold acclimation (4 °C/2 °C, day/night) and 7 days of de-acclimation (12 °C/5 °C, day/night). The results showed that freezing and de-acclimation tolerance are related but genetically distinct. The cold-acclimated (CA) state exhibited significant marker–trait associations on chromosomes 2H and 6H, whereas the de-acclimated (DA) state displayed a broader, more complex genetic architecture, particularly on chromosomes 2H and 7H. F_v/F_m showed the strongest associations for both SNP and DArTseq markers in both states. PI_(csm), followed by PI_(cs0) and PI_(total), showed high SNP associations in the DA state, indicating a strong relationship between photosynthetic performance and freezing tolerance after de-acclimation. Notably, the DArTseq marker 11400277 on chromosome 7H showed multiple marker–trait associations across both states. These findings provide a genomic basis for marker-assisted selection of climate-resilient winter barley cultivars. Full article

Accurate weed mapping in cereal fields requires pixel-level segmentation from unmanned aerial vehicle (UAV) imagery that remains reliable across fields, seasons, and illumination. Existing multispectral pipelines often depend on thresholded vegetation indices, which are brittle under radiometric drift and mixed crop–weed pixels, or on single-stream convolutional neural network (CNN) and Transformer backbones that ingest stacked bands and indices, where radiance cues and normalized index cues interfere and reduce sensitivity to small weed clusters embedded in crop canopy. We propose VISA (Vegetation Index and Spectral Attention), a two-stream segmentation network that decouples these cues and fuses them at native resolution. The radiance stream learns from calibrated five-band reflectance using local residual convolutions, channel recalibration, spatial gating, and skip-connected decoding, which preserve fine textures, row boundaries, and small weed structures that are often weakened after ratio-based index compression. The index stream operates on vegetation-index maps with windowed self-attention to model local structure efficiently, state-space layers to propagate field-scale context without quadratic attention cost, and Slot Attention to form stable region descriptors that improve discrimination of sparse weeds under canopy mixing. To support supervised training and deployment-oriented evaluation, we introduce BAWSeg, a four-year UAV multispectral dataset collected over commercial barley paddocks in Western Australia, providing radiometrically calibrated blue, green, red, red edge, and near-infrared orthomosaics, derived vegetation indices, and dense crop, weed, and other labels with leakage-free block splits. On BAWSeg, VISA achieves 75.6% mean Intersection over Union (mIoU) and 63.5% weed Intersection over Union (IoU) with 22.8 M parameters, outperforming a multispectral SegFormer-B1 baseline by 1.2 mIoU and 1.9 weed IoU. Under cross-plot and cross-year protocols, VISA maintains 71.2% and 69.2% mIoU, respectively. The full BAWSeg benchmark dataset, VISA code, trained model weights, and protocol files will be released upon publication. Full article

Soil tillage significantly affects yield, grain quality, and the environmental footprint of cereals under Mediterranean rainfed conditions. This two-year field study evaluated five contrasting tillage systems: conventional tillage (CT), disc harrow (DH), chisel plough (CP), and two no-tillage systems, including long-term (NT1, 30 years) and recently established (NT2, 3–4 years), for their effects on yield and quality traits, and greenhouse gas (GHG) emissions of malting barley grown in Central Greece. Conventional tillage achieved the highest aboveground biomass (up to 12.1 t ha⁻¹) and yield (up to 6.3 t ha⁻¹), but resulted in lower thousand-grain weight (TGW) and reduced grain plumpness. In contrast, no-tillage systems produced slightly lower yields (4.3–5.2 t ha⁻¹), significantly higher TGW (up to 58.3 g), and improved grain-size distribution, while maintaining grain protein concentration within acceptable malting thresholds (10.4–11.0%). Environmental assessment indicated substantially lower GHG emissions under no-tillage, with NT2 achieving the lowest carbon footprint (0.19–0.22 kg CO₂ eq kg⁻¹). Carbon footprint estimates revealed that carbon accounting tools prioritize short-term management transitions over long-term no-tillage systems. Year effects reflected differences in rainfall distribution and temperature during critical growth stages. Overall, no-tillage systems provided the most balanced agronomic, qualitative, and environmental performance for malting barley under Mediterranean conditions. Full article

Heavy metals in agricultural systems pose a significant challenge to food security, especially in regions with long-term intensive land use. While the Pannonian Plain represents Croatia’s primary breadbasket, accounting for a significant portion of the nation’s cereal production, data on the soil-to-grain transfer of heavy metals and the associated human exposure risk are limited. The objective of this study was (i) to determine the concentrations of arsenic (As), cadmium (Cd), and lead (Pb) in agricultural soils and corresponding grains (wheat, barley, and maize) across four principal counties within the Pannonian region of Croatia; (ii) to evaluate the soil-to-grain transfer factors that varied regionally and among cereal types; and (iii) to assess the potential non-carcinogenic health risks for both adults and children highlighting differences in exposure due to body weight and consumption patterns. Soil and cereal grain samples were collected in 2019 and 2020, and metal concentrations were determined by ICP-MS after microwave acid digestion. The transfer of metals from soil to grain was estimated using the transfer factor (TF), while exposure assessment was conducted by calculating the estimated daily intake (EDI), hazard quotient (HQ), and hazard index (HI). Due to the nonlinear distribution of the data and the lack of strictly matched soil and grain samples, median metal concentrations pooled across all studied regions were used for exposure assessment. For As, a conservative approach was applied, assuming that 50% of the total As is in inorganic form. Additionally, a probabilistic risk assessment using Monte Carlo simulations was conducted to account for variability in body weight and cereal intake, providing a more comprehensive evaluation of potential exposure. The results showed differences in metal accumulation among cereal species, with wheat and barley tending to accumulate more Cd than maize, while As and Pb concentrations in grains were low for all crops studied. Although soil metal concentrations in Međimurje County were generally low, elevated TF values for As and Pb were observed, indicating enhanced soil-to-plant transfer under specific local soil conditions. In contrast, high soil metal concentrations in Slavonski Brod–Posavina County were associated with low TF values, suggesting limited bioavailability and restricted transfer to cereal grains. Both deterministic and probabilistic assessments indicated that the HQ and HI for adults and children were below 1, suggesting low non-carcinogenic risk from cereal consumption. These findings highlight pronounced regional and crop-specific differences in soil-to-plant metal transfer and confirm that low soil contamination does not necessarily imply low transfer potential, emphasizing the importance of integrated soil–plant–grain monitoring for food safety assessment. Full article

There has continued to be an increase in the production of gluten-free products, including beer. This interest is a combination of responses to both consumers addressing food sensitivities as well as personal preferences. Beer produced from gluten-free grains has a distinct flavor that differs greatly from traditional barley beer. Recently, the use of millet to produce gluten-free beer has increased with larger-scale malting of millet. It is the goal of this project to investigate the chemical composition of the millet beer aroma. The fermentation of millet-based beers was compared to sorghum and barley beers. Beyond this, the impact of common yeast strains on the fermentation of millet-based beers weas also investigated. All brews were regularly monitored for pH, gravity, total titratable acidity, total polyphenols, and free amino nitrogen. In addition, the aroma profile was sampled using Solid-Phase Microextraction (SPME) with chemical separation and identification and quantification using Gas Chromatography with Mass Spectroscopy (GC-MS). The analysis showed the production of acceptable beers; however, the fermentation there is obvious needed to optimize brewing conditions. In addition, the amount of total volatile compounds was found to be significantly different than beer produced using malted barley. Full article

Viticulture is a notable economic activity in the Mediterranean basin, and the inter-row area is managed through tillage, which has several disadvantages and can lead to soil erosion. Also, there has been an increased trend in utilizing cover crops in vineyards, as they provide several ecosystem services. The objective of our experiment was to study the growth and yield of monocrops of triticale, barley and pea, and their intercrops when they were grown in a Mediterranean vineyard. The results show that pea–triticale and pea–barley intercropping systems exhibited higher or earlier peaks in leaf area index (up to 180%) than monocultures, indicating complementary canopy structures that improved light interception. Intercrops consistently produced higher biomass, with triticale–pea yielding up to 11.63 t ha⁻¹, though grain yield was more variable and sensitive to environmental stresses during reproductive stages. The indices that were determined showed the significant advantage of the intercrops compared to the monocrops. Also, intercrops showed higher environmental resource use efficiency, as measured with Radiation Use Efficiency (RUE) and Water Use Efficiency (WUE), compared to the monocrops. The present study demonstrates that cereal–legume intercropping in vineyards can increase biomass, grain production, and environmental resource use efficiency and can be used for sustainable intensification in Mediterranean cropping systems. Full article