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Keywords = bamboo nutrition

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16 pages, 1618 KiB  
Article
Sustainable Bamboo-Based Packaging and Passive Modified Atmosphere: A Strategy to Preserve Strawberry Quality During Cold Storage
by Giuseppina Adiletta, Marisa Di Matteo, Giuseppe De Filippis, Antonio Di Grazia, Paolo Ciambelli and Milena Petriccione
Processes 2025, 13(7), 2262; https://doi.org/10.3390/pr13072262 - 15 Jul 2025
Viewed by 340
Abstract
This study investigates the potential of bamboo-based sustainable packaging in combination with passive modified atmosphere (MA) and cold storage to enhance the shelf life of strawberries while preserving their physico-chemical properties, bioactive compounds, and antioxidant enzyme activity. The study monitored key parameters such [...] Read more.
This study investigates the potential of bamboo-based sustainable packaging in combination with passive modified atmosphere (MA) and cold storage to enhance the shelf life of strawberries while preserving their physico-chemical properties, bioactive compounds, and antioxidant enzyme activity. The study monitored key parameters such as fruit weight loss, firmness, color, and the content of bioactive compounds as well as phenolics and flavonoids. Additionally, antioxidant enzyme activity, including catalase, ascorbate peroxidase, and superoxide dismutase, was assessed to evaluate oxidative stress during 9 days at 4 °C. The results show that strawberries packaged with bamboo materials in a passive MA retained their physico-chemical traits, exhibiting slower changes in firmness, color, and bioactive compound content compared to those in unpackaged samples. Furthermore, the antioxidant enzyme activity remained significantly higher, suggesting a lower oxidative stress in packaged fruit. This combination of bamboo-based packaging with passive MA is a valid, effective, and sustainable approach to prolonging the qualitative traits of strawberries during cold storage, offering both environmental and nutritional benefits. Full article
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17 pages, 4191 KiB  
Article
Calcium Supplement Combined with Dietary Supplement Kidtal Can Promote Longitudinal Growth of Long Bone in Calcium-Deficient Adolescent Rats
by Haosheng Xie, Mingxuan Zhang, Zhengyuan Zhou, Hongyang Guan, Kunmei Shan, Shiwei Mi, Xinfa Ye, Zhihui Liu, Jun Yin and Na Han
Nutrients 2025, 17(12), 1966; https://doi.org/10.3390/nu17121966 - 10 Jun 2025
Viewed by 995
Abstract
Objective: Growth retardation in adolescents caused by nutritional deficiency requires effective intervention. A novel dietary supplement containing bamboo shoot extract, amino acids, and calcium citrate (Kidtal + Ca, KDTCa) was evaluated for its growth-promoting effects. Methods: After acclimatization, sixty-three 3-week-old male Sprague-Dawley (SD) [...] Read more.
Objective: Growth retardation in adolescents caused by nutritional deficiency requires effective intervention. A novel dietary supplement containing bamboo shoot extract, amino acids, and calcium citrate (Kidtal + Ca, KDTCa) was evaluated for its growth-promoting effects. Methods: After acclimatization, sixty-three 3-week-old male Sprague-Dawley (SD) rats were randomly divided into a normal control group and model groups. Growth retardation was induced in the modeling groups through calcium-deficient feeding, followed by administration of KDTCa, bamboo shoot extract and amino acids (Kidtal), or calcium citrate (CC). After 6 weeks of intragastric administration, the mechanical properties, microstructure, and growth plate development of bone were evaluated using three-point bending, micro-CT, and H&E staining, respectively. Bone calcium/phosphorus distribution and fecal calcium apparent absorption rate were measured by ICP-MS. Results: All inter-group differences were analyzed using one-way analysis of variance and checked using the Tuckey test. KDTCa treatment dose-dependently enhanced bone development in calcium-deficient rats. Compared to the model group, H-KDTCa significantly restored naso-anal length (p < 0.05) and body weight (p < 0.01). KDTCa supplementation significantly restored calcium and phosphorus levels in blood and bone. Three-point bending experiments showed that the stiffness and bending energy were increased by 142.58% and 384.7%. In bone microarchitecture, both bone mineral density (BMD) and microstructural parameters were significantly improved. These findings were consistent with the increased long bone length (p < 0.05) and decreased serum BALP/TRACP levels (p < 0.001). Dose-dependent IGF-1 elevation (p < 0.01) potentially mediated growth plate elongation by 35.34%. Notably, KDTCa increased calcium apparent absorption by 6.1% versus calcium-only supplementation at equal intake. Conclusions: KDTCa improves bone microstructure and strength, restores bone metabolism, and enhances growth plate height via promoting IGF-1 secretion to facilitate bone development. Further studies are needed to determine whether the components and calcium in Kidtal have a synergistic effect. Full article
(This article belongs to the Section Micronutrients and Human Health)
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20 pages, 34946 KiB  
Article
Feasibility of Bamboo Sawdust as Sustainable Alternative Substrate for Auricularia heimuer Cultivation
by Ya-Hui Wang, Cong-Sheng Yan, Yong-Jin Deng, Zheng-Fu Zhu, Hua-An Sun, Hui-Ping Li, Hong-Yuan Zhao and Guo-Qing Li
J. Fungi 2025, 11(5), 387; https://doi.org/10.3390/jof11050387 - 17 May 2025
Viewed by 724
Abstract
With the increasing scarcity of traditional hardwood sawdust resources, developing sustainable substrates for edible fungi cultivation has become an urgent industrial priority. This study systematically evaluated the effects of bamboo sawdust substitutions (20%, 30%, 40%, and 50%) on mycelial growth, fruiting body development, [...] Read more.
With the increasing scarcity of traditional hardwood sawdust resources, developing sustainable substrates for edible fungi cultivation has become an urgent industrial priority. This study systematically evaluated the effects of bamboo sawdust substitutions (20%, 30%, 40%, and 50%) on mycelial growth, fruiting body development, and nutritional quality of Auricularia heimuer, while elucidating the underlying molecular mechanisms through transcriptome sequencing. The results demonstrated that bamboo substitution of ≤30% maintained normal mycelial growth and fruiting body differentiation, with 20% and 30% substitutions increasing yields by 5.30% and 3.70%, respectively, compared to the control. However, 50% substitution significantly reduced yield by 9.49%. Nutritional analysis revealed that 20–40% bamboo substitution significantly enhanced the contents of crude protein, polysaccharides, and essential minerals (calcium, iron, and selenium) in fruiting bodies. Transcriptome analysis identified upregulation of glycosyl hydrolase family genes and downregulation of redox-related genes with increasing bamboo proportions. Biochemical assays confirmed these findings, showing decreased oxidative substances and increased reductive compounds in mycelia grown with high bamboo content, which indicate disrupted cellular redox homeostasis. This study provides both a practical solution to alleviate the “edible mushrooms derived from lignicolous fungi–forest conflict” and fundamental insights into fungal adaptation mechanisms to non-wood substrates, thus establishing a theoretical foundation for the valorization of agricultural and forestry wastes. Full article
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18 pages, 2613 KiB  
Review
Research Advances in Underground Bamboo Shoot Detection Methods
by Wen Li, Qiong Shao, Fan Guo, Fangyuan Bian and Huimin Yang
Agronomy 2025, 15(5), 1116; https://doi.org/10.3390/agronomy15051116 - 30 Apr 2025
Viewed by 1204
Abstract
Underground winter bamboo shoots, prized for their high nutritional value and economic significance, face harvesting challenges owing to inefficient manual methods and the lack of specialized detection technologies. This review systematically evaluates current detection approaches, including manual harvesting, microwave detection, resistivity methods, and [...] Read more.
Underground winter bamboo shoots, prized for their high nutritional value and economic significance, face harvesting challenges owing to inefficient manual methods and the lack of specialized detection technologies. This review systematically evaluates current detection approaches, including manual harvesting, microwave detection, resistivity methods, and biomimetic techniques. While manual methods remain dominant, they suffer from labor shortages, low efficiency, and high damage rates. Microwave-based technologies demonstrate high accuracy and good depths but are hindered by high costs and soil moisture interference. Resistivity methods show feasibility in controlled environments but struggle with field complexity and low resolution. Biomimetic approaches, though innovative, face limitations in odor sensitivity and real-time data processing. Key challenges include heterogeneous soil conditions, performance loss, and a lack of standardized protocols. To address these, an integrated intelligent framework is proposed: (1) three-dimensional modeling via multi-sensor fusion for subsurface mapping; (2) artificial intelligence (AI)-driven harvesting robots with adaptive excavation arms and obstacle avoidance; (3) standardized cultivation systems to optimize soil conditions; (4) convolution neural network–transformer hybrid models for visual-aided radar image analysis; and (5) aeroponic AI systems for controlled growth monitoring. These advancements aim to enhance detection accuracy, reduce labor dependency, and increase yields. Future research should prioritize edge-computing solutions, cost-effective sensor networks, and cross-disciplinary collaborations to bridge technical and practical gaps. The integration of intelligent technologies is poised to transform traditional bamboo forestry into automated, sustainable “smart forest farms”, addressing global supply demands while preserving ecological integrity. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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21 pages, 1441 KiB  
Review
Bamboo: Global Occurrence and Its Significance as Food and Related Products
by Utsaphong Uprarawanna, Jiraphat Kaewsritong and Khongsak Srikaeo
Crops 2025, 5(2), 11; https://doi.org/10.3390/crops5020011 - 17 Mar 2025
Viewed by 3113
Abstract
Bamboo has recently garnered international recognition for its nutritional and medicinal properties, which contribute significantly to the culinary, pharmaceutical, and cosmeceutical sectors. Every component of the bamboo plant—rhizome, culm, bark shavings, shoots, leaves, roots, and seeds—possesses useful applications. Bamboo shoots, as a type [...] Read more.
Bamboo has recently garnered international recognition for its nutritional and medicinal properties, which contribute significantly to the culinary, pharmaceutical, and cosmeceutical sectors. Every component of the bamboo plant—rhizome, culm, bark shavings, shoots, leaves, roots, and seeds—possesses useful applications. Bamboo shoots, as a type of food, have been consumed for a long time due to their nutritional and edible qualities, as well as their economic value. Other parts of bamboo, such as leaves and culms, can also be used in food applications. Commercial markets in various countries offer a variety of bamboo-based products, including those that are dried, fermented, pickled, and thermally processed. This article provides an overview of the use of all edible bamboo parts as foods and related products as well as their nutritional benefits. A wide range of traditional food products derived from bamboo, including industrial products and some novel products, was investigated. It is possible that the promotion of bamboo as a staple food could offer substantial promise in addressing the worldwide concerns regarding hunger, food insecurity, and environmental degradation. Full article
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22 pages, 5644 KiB  
Article
Biochar and Ammonium Nitrate Synergies: Enhancing Nitrogen Availability and Maize Growth in Oxisols
by Igor de Oliveira Paiva, Everton Geraldo de Morais and Carlos Alberto Silva
Agronomy 2025, 15(3), 633; https://doi.org/10.3390/agronomy15030633 - 2 Mar 2025
Cited by 1 | Viewed by 1526
Abstract
Effective nitrogen (N) management and the development of novel N fertilizers are essential for enhancing maize growth in tropical soils. One strategy to increase N use efficiency is the use of organic matrices as a source of N or their combination with the [...] Read more.
Effective nitrogen (N) management and the development of novel N fertilizers are essential for enhancing maize growth in tropical soils. One strategy to increase N use efficiency is the use of organic matrices as a source of N or their combination with the application of mineral N sources. Among these organic matrices, biochar emerges as a highly promising option for optimizing N use efficiency. Thus, the aim of this study was to evaluate the effects of different feedstocks, their respective biochars, and their combination with N on the dynamics and uptake of N by maize plants in two contrasting Oxisols. A 30-day greenhouse experiment was conducted using maize grown under treatments with four feedstocks (bamboo, sunflower cake, chicken manure, and shrimp carcass) and their respective biochars. The biochars were applied with or without ammonium nitrate (AN), alongside negative (no N) and positive (AN-only) controls. Ammonium and nitrate levels were analyzed in the soil solution at 1 and 15 days and in the whole soil before and after cultivation. Maize biomass production and shoot N accumulation were also evaluated at the end of the experiment. Among the main results, it was observed that soil type played a key role in available N, maize nutrition, and growth. In the medium-textured Oxisol studied, native soil organic matter partially met maize N requirements due to high content of available N observed. Biochars influenced N availability by increasing nitrate-N prevalence in the soil solution. Although whole-soil N levels were sufficient for robust maize growth, post-cultivation residual N remained low (<75 mg kg−1), indicating the need for supplemental N fertilization for plants grown in pots. In the medium-textured Oxisol, bamboo or sunflower cake biochar combined with AN increased biomass production by ~12% compared with AN alone. Similarly, in the clayey Oxisol, maize fertilized with sunflower cake or shrimp carcass biochar—regardless of AN addition—outperformed AN-fertilized plants by 19–30%. Thus, this study highlights the potential of integrating biochar with N fertilization to improve soil and solution N availability and increase N use efficiency by maize plants. Full article
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19 pages, 5720 KiB  
Article
Short-Term Effects of Three Tree Species on Soil Physicochemical Properties and Microbial Communities During Land-Use Change from Farmland to Forests
by Yi Jian, Jing Lin, Changlong Mu, Yuqi Wang, Zhenyang He, Gang Chen and Wei Ding
Forests 2025, 16(2), 362; https://doi.org/10.3390/f16020362 - 17 Feb 2025
Cited by 2 | Viewed by 624
Abstract
In recent decades, much of China’s farmland has been transformed into forests due to the Conversion of Farmland to Forests and Grasses Project. While past research has mainly examined soil nutrients and water conservation, less attention has been given to soil microbial communities. [...] Read more.
In recent decades, much of China’s farmland has been transformed into forests due to the Conversion of Farmland to Forests and Grasses Project. While past research has mainly examined soil nutrients and water conservation, less attention has been given to soil microbial communities. This study examined the effects of converting farmland to forests of Pleioblastus amarus (PA), Populus deltoides (PD), or Zanthoxylum bungeanum (ZB) on the soil physiochemical properties, enzymes, and microbial communities, using abandoned land (AL) as the control, over a period of five years. The results showed that PA increased the soil organic carbon (SOC) content, although not significantly, while significantly boosting the C:N and C:P ratios and urease activity compared to the AL. PD notably reduced the amylase and cellulase activities, as well as the fungal Shannon index. Additionally, the beta diversity of both the bacterial and fungal communities in the PA stand was clearly distinct from that of the AL and the other tree species. The SOC content, total potassium content, and cellulase activity showed significant correlations with bacterial communities. Moreover, the bacterial community changes in the PD and ZB stands were mainly driven by the genera Steroidobacter, Roseisolibacter, and Serendipita, and were negatively correlated with the SOC content, C:N and C:P ratios, and cellulase activity. In contrast, the fungal community changes in the PA stand were primarily influenced by the order Capnodiales, family Capnodiaceae, genus Chaetocapnodium, and species Chaetocapnodium philippinense, which were positively correlated with the soil pH, C:N and C:P ratios, and cellulase activity. Furthermore, “Metabolism” was identified as the primary bacterial function, and converting farmland to forest altered the fungal nutritional type from Saprotroph to Pathotroph–Saprotroph–Symbiotroph, particularly in the PA stand. These findings indicate that converting farmland to forest, particularly with bamboo P. amarus, significantly impacts the bacterial and fungal communities in the soil and changes the fungal trophic type due to the carbon source and cellulase activity of this tree species. Full article
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20 pages, 5144 KiB  
Article
Grazing-Induced Habitat Degradation: Challenges to Giant Panda Survival Resulting from Declining Bamboo and Soil Quality
by Huawei Tian, Ying Zeng, Zejun Zhang, Ming Lu and Wei Wei
Animals 2025, 15(2), 202; https://doi.org/10.3390/ani15020202 - 14 Jan 2025
Cited by 1 | Viewed by 1322
Abstract
Grazing is the primary human-induced disturbance affecting giant panda (Ailuropoda melanoleuca) habitats and has a severe impact on the long-term sustainability of the giant panda population. To address the lack of quantitative studies on grazing’s impact on habitat quality, we selected [...] Read more.
Grazing is the primary human-induced disturbance affecting giant panda (Ailuropoda melanoleuca) habitats and has a severe impact on the long-term sustainability of the giant panda population. To address the lack of quantitative studies on grazing’s impact on habitat quality, we selected China’s most heavily grazed giant panda nature reserve. Utilizing the Maxent model and stoichiometric analysis, we investigated habitat quality degradation caused by grazing and quantified changes in bamboo nutritional quality and soil physicochemical properties. The results indicate that grazing has significantly reduced the suitable habitat area for giant pandas from 101.87 km2 to 80.64 km2. Specifically, high-suitability habitats declined by 14.14%, moderate-suitability habitats declined by 22.70%, and low-suitability habitats declined by 22.88%. Grazing has forced pandas to move to higher altitudes (2650–3057 m) with taller (12–20 m) trees, denser (28–55 plants) shrubs, and sparser (30–69%) bamboo. Additionally, the soil water content has decreased, while soil bulk density, total N, available N, and pH have significantly increased. Reductions in crude protein and ether extract, along with increased crude fiber and ash, have lowered bamboo’s nutritional value and palatability. This study elucidates how grazing degrades giant panda habitat quality and provides a scientific basis for its conservation management. Full article
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16 pages, 4410 KiB  
Article
Effects of Ecological Restoration Measures on Growth Renewal and Nutritional Quality of Arundinaria faberi in Degraded Habitat of Giant Panda
by Weirui Qin, Jingyi Liu, Han Pan, Yong Cheng, Xinqiang Song, Mingxia Fu, Yuanbin Zhang, Xiaofeng Luan and Biao Yang
Forests 2024, 15(12), 2241; https://doi.org/10.3390/f15122241 - 20 Dec 2024
Cited by 1 | Viewed by 934
Abstract
Restoring the degraded habitat of the giant panda (Ailuropoda melanoleuca) is of paramount importance for the conservation of the species and its forest ecosystem. However, little is known about the impact of ecological restoration interventions on the growth renewal and nutritional [...] Read more.
Restoring the degraded habitat of the giant panda (Ailuropoda melanoleuca) is of paramount importance for the conservation of the species and its forest ecosystem. However, little is known about the impact of ecological restoration interventions on the growth renewal and nutritional quality of Arundinaria faberi in the degraded habitat of the giant panda. Here, we implemented strip thinning and blocky thinning techniques in the Daxiangling mountain range, alongside a control group. A random forest model and multiple linear regression analysis were employed to predict changes in the growth renewal and nutritional quality of bamboo, particularly in the strip-thinned zones. The key findings were as follows: (1) Compared with the control area, strip thinning increased the number of shoots and increased the diameter and height of shoots. (2) The random forest model predicted a decline in bamboo regeneration indices in 2023 compared to 2022 under strip thinning. (3) Through thinning, the palatability and nutritional level of A. faberi were improved. (4) Long-term effects included increased tannin in leaves, decreased tannin and amino acids in shoots and culms, and increased crude fat, with changes in crude protein distribution across bamboo parts. Overall, these findings provide valuable insights for habitat restoration efforts targeting giant panda populations in the low-canopy forest ecosystems of the Daxiangling mountain range. Full article
(This article belongs to the Section Forest Biodiversity)
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26 pages, 7275 KiB  
Review
A Review of the Nutritional Composition, Storage Challenges, Processing Technology and Widespread Use of Bamboo Shoots
by Ting Ma, Wenfeng Mo, Beibei Lv, Wenxuan Wang, Hailin He, Cuiwen Jian, Xiaoling Liu, Shubo Li and Yuan Guo
Foods 2024, 13(22), 3539; https://doi.org/10.3390/foods13223539 - 6 Nov 2024
Cited by 5 | Viewed by 4294
Abstract
Bamboo shoots, as the young bamboo stems, are rich in protein, fiber, vitamins, and minerals, as well as many bioactive substances beneficial to health, and are gaining in importance worldwide as a healthy food and dietary supplement. However, fresh bamboo shoots lignify rapidly [...] Read more.
Bamboo shoots, as the young bamboo stems, are rich in protein, fiber, vitamins, and minerals, as well as many bioactive substances beneficial to health, and are gaining in importance worldwide as a healthy food and dietary supplement. However, fresh bamboo shoots lignify rapidly after harvesting and contain cyanogenic glycosides, limiting the safe and healthy consumption of bamboo shoots. To this end, based on the changes in nutritional composition and the physiological properties of fresh and post-harvest bamboo shoots, factors affecting the preservation of post-harvest bamboo shoots are emphasized, including a series of physical and chemical regimes and various processing methods for post-harvest preservation. Furthermore, a systematic biorefinery approach for using bamboo shoot processing residue to prepare value-added products is also discussed. Finally, the article also discusses issues related to sustainable development, safeguarding food security, and addressing potential health impacts in order to provide a scientific basis for researchers to further develop and increase the added value of bamboo shoots. Full article
(This article belongs to the Section Food Engineering and Technology)
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15 pages, 2491 KiB  
Article
Integrated Metabolome and Transcriptome Analysis Reveals the Key Bitter Substances of Pleioblastus amarus Shoots at Different Developmental Stages
by Limei Gong, Weijin Shi, Chunyan Mou, Shixing Zhou, Lin Xiao, Hongling Hu, Lihua Tu, Guirong Hou, Wei Ding, Congde Huang and Gang Chen
Horticulturae 2024, 10(11), 1161; https://doi.org/10.3390/horticulturae10111161 - 31 Oct 2024
Cited by 3 | Viewed by 1215
Abstract
Pleioblastus amarus (bitter bamboo) shoots are a traditional forest vegetable in China, renowned for its nutritional composition and associated health benefits. This study aimed to investigate the dynamic changes in nutritional quality and bitterness-related metabolites, as well as corresponding gene expression in P. [...] Read more.
Pleioblastus amarus (bitter bamboo) shoots are a traditional forest vegetable in China, renowned for its nutritional composition and associated health benefits. This study aimed to investigate the dynamic changes in nutritional quality and bitterness-related metabolites, as well as corresponding gene expression in P. amarus shoots at three distinct growth stages, specifically at heights of 10 cm (BT1), 20 cm (BT2), and 40 cm (BT3). The results showed that the content of soluble sugars decreased while the content of total phenols and flavonoids, which contribute to bitterness, increased during the growth of P. amarus shoots. In addition, comparative analysis of BT2 vs. BT1, BT3 vs. BT1, and BT3 vs. BT2 revealed 14, 43, and 36, respectively, differentially accumulated metabolites (DAMs). Notably, BT2 exhibited upregulation of flavonoids, such as apigenin, while bitter amino acids, including phenylalanine and arginine, showed a marked increase in BT3. Moreover, differential gene expression analysis revealed 3161, 13268, and 3742 differentially expressed genes (DEGs) in the BT2 vs. BT1, BT3 vs. BT1, and BT3 vs. BT2 comparisons, respectively. An integrated analysis of metabolomic and transcriptomic data indicated that the metabolites and genes associated with the biosynthesis pathways of phenylalanine, tyrosine, tryptophan, arginine, and the branched-chain amino acids valine, leucine, and isoleucine were significantly enriched during the growth of P. amarus shoots. The study indicates that early-stage (≤20 cm) P. amarus shoots are mainly bitter due to flavonoids like apigenin, while the bitterness in later-stage (around 40 cm) shoots is largely due to bitter amino acids such as phenylalanine, arginine, valine, and isoleucine. This research highlights key compounds and genes related to bitterness, providing a basis for future research on enhancing the nutritional value and flavor of P. amarus shoots. Full article
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18 pages, 8031 KiB  
Article
Identification of New Cultivar and Different Provenances of Dendrocalamus brandisii (Poaceae: Bambusoideae) Using Simple Sequence Repeats Developed from the Whole Genome
by Ruiman Geng, Junlei Xu, Jutang Jiang, Zhanchao Cheng, Maosheng Sun, Nianhe Xia and Jian Gao
Plants 2024, 13(20), 2910; https://doi.org/10.3390/plants13202910 - 17 Oct 2024
Cited by 1 | Viewed by 1107 | Correction
Abstract
Dendrocalamus brandisii is a high-quality bamboo species that can be used for both bamboo shoots and wood. The nutritional components and flavors of D. brandisii vary from different geographical provenances. However, the unique biological characteristics of bamboo make morphological classification methods unsuitable for [...] Read more.
Dendrocalamus brandisii is a high-quality bamboo species that can be used for both bamboo shoots and wood. The nutritional components and flavors of D. brandisii vary from different geographical provenances. However, the unique biological characteristics of bamboo make morphological classification methods unsuitable for distinguishing them. Although the new cultivar ‘Manxie No.1’ has significant differences in the branch characteristics and the color of shoot sheaths compared to the D. brandisii, it still lacks precise genetic information at the molecular level. This study identified 231,789 microsatellite markers based on the whole genome of D. brandisii and analyzed their type composition and distribution on chromosomes in detail. Then, using TP-M13-SSR fluorescence-labeling technology, 34 pairs of polymorphic primers were screened to identify the new cultivar ‘Manxie No.1’ and 11 different geographical provenances of D. brandisii. We also constructed DNA fingerprinting profiles for them. At the same time, we mapped six polymorphic SSRs to the gene of D. brandisii, among which SSR673 was mapped to DhB10G011540, which is related to plant immunity. The specific markers selected in this study can rapidly identify the provenances and the new cultivar of D. brandisii and help explore candidate genes related to some important traits. Full article
(This article belongs to the Special Issue The Genetic Architecture of Bamboo Growth and Development)
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16 pages, 2084 KiB  
Article
A Dietary Supplement in the Management of Patients with Lumbar Osteochondrosis: A Randomized, Double-Blinded, Placebo-Controlled Study
by Brenda Laky, Daniel Huemer, Martin Eigenschink, Benedikt Sagl, Rainer Thell, Karl-Heinz Wagner, Werner Anderl and Philipp R. Heuberer
Nutrients 2024, 16(16), 2695; https://doi.org/10.3390/nu16162695 - 14 Aug 2024
Cited by 1 | Viewed by 5655
Abstract
Various nutritional supplements are available over the counter, yet few have been investigated in randomized controlled trials. The rationale for using the specific mix of nutritional substances including collagen type II, hyaluronic acid, n-acetyl-glucosamine, bamboo extract, L-lysine, and vitamin C is the assumption [...] Read more.
Various nutritional supplements are available over the counter, yet few have been investigated in randomized controlled trials. The rationale for using the specific mix of nutritional substances including collagen type II, hyaluronic acid, n-acetyl-glucosamine, bamboo extract, L-lysine, and vitamin C is the assumption that combining naturally occurring ingredients of the intervertebral disc would maintain spine function. This double-blinded, placebo-controlled randomized trial aimed to evaluate the efficacy of a nutraceutical supplement mix in the management of lumbar osteochondrosis. Fifty patients were randomly assigned to either the supplement or placebo group in a 1:1 ratio. Patient-Reported Outcome Measures (PROMs) included the Oswestry Disability Index (ODI), the visual analogue scale for pain (pVAS), short form-12 (SF-12) physical and mental component summary subscale scores (PCS and MCS, respectively), and global physical activity questionnaire (GPAQ). Magnetic resonance imaging (MRI) was used to evaluate degenerative changes of intervertebral discs (IVD) including Pfirrmann grades as well as three-dimensional (3D) volume measurements. Data were collected at baseline and after the 3-month intervention. None of the PROMs were significantly different between the supplement and placebo groups. Disc degeneration according to Pfirrmann classifications remained stable during the 3-month intervention in both groups. Despite no significance regarding the distribution of Pfirrmann grade changes (improvement, no change, worsening; p = 0.259), in the supplement group, one patient achieved a three-grade improvement, and worsening of Pfirrmann grades were only detected in the placebo group (9.1%). Furthermore, in-depth evaluations of MRIs showed significantly higher 3D-measured volume changes (increase) in the supplement (+740.3 ± 796.1 mm3) compared to lower 3D-measured volume changes (decrease) in the placebo group (−417.2 ± 875.0 mm3; p < 0.001). In conclusion, this multi-nutrient supplement might not only stabilize the progression of lumbar osteochondrosis, but it might also potentially even increase IVD volumes as detected on MRIs. Full article
(This article belongs to the Section Micronutrients and Human Health)
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15 pages, 3588 KiB  
Article
Nutrient Utilization and Gut Microbiota Composition in Giant Pandas of Different Age Groups
by Chengdong Wang, Wenwen Deng, Zhi Huang, Caiwu Li, Rongping Wei, Yan Zhu, Kai Wu, Chengyao Li, Linhua Deng, Ming Wei, Xuemei Chen and Desheng Li
Animals 2024, 14(16), 2324; https://doi.org/10.3390/ani14162324 - 12 Aug 2024
Cited by 1 | Viewed by 1654
Abstract
Proper feeding and nutrition are vital for maintaining the health of giant pandas (GPs), yet the impact of dietary changes and gut microbiota on their nutrient utilization remains unclear. To address these uncertainties, we investigated nutrient intake and apparent digestibility, as well as [...] Read more.
Proper feeding and nutrition are vital for maintaining the health of giant pandas (GPs), yet the impact of dietary changes and gut microbiota on their nutrient utilization remains unclear. To address these uncertainties, we investigated nutrient intake and apparent digestibility, as well as gut microbiota composition across different age groups of giant pandas: sub-adults (SGPs), adults (AGPs), and geriatrics (GGPs). Our findings revealed notable shifts in dietary patterns from SGPs to GGPs. As they aged, significantly more bamboo shoots and less bamboo were consumed. Consequently, GGPs showed significantly reduced crude fiber (CF) intake and digestibility, while crude protein (CP) did not alter significantly. In addition, 16S rRNA microbial sequencing results showed that unidentified_Enterobacteriaceae and Streptococcus were the dominant genera among all age groups. The relative abundance of the genus Enterococcus in GGPs was significantly higher than that in SGPs and AGPs (p < 0.05). Overall, our results indicated the importance of bamboo shoots as a major source of protein in GGPs’ diet, which can effectively compensate for the certain nutritional loss caused by the reduction in bamboo intake. Age-related changes in bacterial abundance have an effect on specific nutrient apparent digestibility in the gut of GPs. The data presented in this study serve as a useful reference for nutritional management in different ages of GPs under healthy conditions. Full article
(This article belongs to the Section Mammals)
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14 pages, 36755 KiB  
Article
Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage
by Xin Zhao, Wenjuan Song, Sen Chen, Gang Xu, Zhijian Long, Heyi Yang, Ying Cao and Shanglian Hu
Int. J. Mol. Sci. 2024, 25(15), 8065; https://doi.org/10.3390/ijms25158065 - 24 Jul 2024
Cited by 4 | Viewed by 1131
Abstract
Dendrocalamus farinosus bamboo shoots, a species with rich nutritional value, are important in Southwest China. Lignin is an important factor affecting the postharvest flavor quality of bamboo shoots; however, the underlying mechanism of lignin deposition in D. farinosus bamboo shoots during cold storage is [...] Read more.
Dendrocalamus farinosus bamboo shoots, a species with rich nutritional value, are important in Southwest China. Lignin is an important factor affecting the postharvest flavor quality of bamboo shoots; however, the underlying mechanism of lignin deposition in D. farinosus bamboo shoots during cold storage is still not fully understood. In this study, the mutant D. farinosus XK4 with low lignin content at 3.11% and the cultivated variety ZPX at 4.47% were used as experimental materials. The lignin content of D. farinosus XK4 and ZPX, as well as the gene expression differences between them, were compared and analyzed during cold storage using transcriptomic and physiological methods. Our analysis revealed several key genes and found that D. farinosus CCoAOMT1 plays a key role in the regulatory network of bamboo shoots during cold storage. Tobacco heterologous transformation experiments demonstrated that overexpression of DfCCoAOMT1 significantly increases lignin content. This study provides a novel foundation for future research aimed at improving the postharvest quality and flavor of D. farinosus bamboo shoots through targeted genetic manipulation during cold storage. Full article
(This article belongs to the Special Issue Advances in Plant Metabolite Research)
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