Search Results (328)

Introduction: Biological invasions constitute one of the main threats to freshwater ecosystems, causing significant ecological changes through the introduction of exotic species that compete with or prey upon native species. In Portugal, the introduction and spread of exotic species in lotic and lentic ecosystems, such as pike (Esox lucius), European catfish (Silurus glanis), and largemouth bass (Micropterus salmoides), all top predators, may have a big impact on autochthonous species. In contrast, bleak (Alburnus alburnus), European perch (Perca fluviatilis), and common carp (Cyprinus carpio) compete aggressively for food resources. In the Sousa River basin, gudgeon (Gobio lozanoi) is considered an exotic species with potential ecological impact, with the minnow (Phoxinus phoxinus) stand having been recently identified in Portugal and, so far, recorded only in this river basin, and not yet being classified as an invasive species in Portugal. Public knowledge regarding invasive aquatic biodiversity remains a significant bottleneck for conservation. Because recreational angling is a prominent dispersal vector, initiatives that directly target this community are relevant. Objective: The aim is to carry out a bibliographic review on the exotic ichthyofauna species present in the Sousa River, with special focus on the invasion potential of the minnow (P. phoxinus). Methodology: The literature review was conducted based on the ScienceDirect, Springer Nature Link, and Fauna Norvegica databases, selecting publications between 2006 and 2025 concerning relevant studies on the potentially invasive characteristics of the minnow (P. phoxinus). The methodology is based on the analysis of studies regarding the impacts caused on riparian ecosystems. Results: The species P. phoxinus presents a generalist diet and high adaptive capacity, allowing it to colonise new habitats and compete aggressively with native species for trophic resources. Its presence is associated with negative impacts on brown trout populations (Salmo trutta), reducing growth and productivity, especially in mountain ecosystems. Increased species density also causes a significant decrease in benthic macroinvertebrate biodiversity. Studies conducted in the Douro basin indicate that the arrival of minnow in Portugal resulted from human action, probably associated with its use as live bait in recreational fishing. Conservation programmes use diverse tactics to bridge the awareness gap. Recent initiatives feature electrofishing demonstrations to visually differentiate species, theatrical performances, and even culinary show-cooking events using invasive predators like the European catfish to promote harvesting. Conclusions: The potential transition of P. phoxinus into an exotic and invasive species may be associated with the ecological pressure exerted on native communities, particularly through competition for trophic resources, highlighting the need to assess its dispersion in the Sousa basin and its impacts on fish fauna and benthic macroinvertebrates. It is important to do more sampling to understand its real distribution in the Sousa Basin. Additionally is important to explain to recreational anglers and the general population the impacts of fish transfer and the adverse effects of invasive species on freshwater Portuguese ecosystems. Full article

The spread of non-native freshwater turtles in urban, peri-urban, and natural environments poses increasing ecological and sanitary concerns, particularly due to their potential role as reservoirs of infectious agents. Among these, DNA viruses remain largely unexplored in both invasive and native chelonians. In this study, a molecular survey targeting selected viral pathogens was conducted on oral and cloacal swabs collected from non-native freshwater turtles from natural and confinement ponds in Northeastern Italy, with the aim of assessing the pathogen’s presence and their potential epidemiological relevance. One hundred sixty-four pond sliders (Trachemys scripta) were sampled from three sites: Herpesviruses and ranaviruses were not detected; in contrast, adenoviruses were frequently identified (72/163, 44.2%). Sequence analyses allowed their classification mostly as Testadenovirus trachemys, with only a single detection of a strain closely related to siadenoviruses and previously associated with mortality events in other tortoise species. Although the pathogenic significance of these viruses remains unclear, their detection highlights the potential role of non-native turtles as viral carriers and underlines the need for systematic virological surveillance in non-native species, particularly in ecosystems shared with susceptible native fauna. Full article

Introduction: Fish species represent 25% of all vertebrates across the globe and are one of the most threatened animal groups. At least 40% of the fish fauna occurs in rivers for part of their life cycle. European rivers are home to more than 600 fish species, while also being some of the most impaired and altered ecosystems. Objective: The objective was to assess the vulnerability of freshwater fish communities in European river basins. Methodology: Using RivTool and the CCM2 database, we developed the River Restoration Units (R2Us), a set of spatial units that takes into account river network functioning and allows a higher spatial discretisation than river basins. We developed RivFish, a database about the presence of native freshwater-dependent fish in 1556 Europeans river basins. For this, we collected data from 77 references and validated synonyms and scientific names for 667 species. We used the latest International Union for Conservation of Nature (IUCN) Red List assessment to define species distributions in European rivers. After intersecting with the R2U layer, we curated and validated species names and spatial occurrence using RivFish. To map the vulnerability of freshwater fish communities, we used the Habitats Directive (HD) and the IUCN datasets. These consider a distinct number of species and assess conservation status differently: the HD evaluated 165 species, while the IUCN evaluated 516 species. The HD data allowed calculating the composite indicator of Conservation Status, whereas the IUCN data enabled calculating the vulnerability index. Results: Both ana-lyses show higher richness in central Europe, particularly in the Danube basin. Spatially, both highlight southern Europe as the area where fish communities have the highest vulnerability. However, the HD analysis also indicates the Danube and the western Atlantic basins as having high vulnerability. The IUCN analysis shows the Anatolian and Mediterranean biogeographical regions as those with the highest vulnerability values. Conclusions: Southern Europe’s higher vulnerability is likely associated with restricted distribution ranges and high levels of endemicity in Mediterranean fish communities. Overall, these findings improve current knowledge and show that input data may be key to effort allocation towards the management and conservation of European freshwater fish communities. Full article

The need for reliable biological indicators to support soil biomonitoring is increasing, particularly in agricultural systems where management practices and environmental pressures interact to influence soil ecological integrity. Among soil fauna-based indicators that measure soil health, the Soil Biological Quality indices based on microarthropods (QBS-ar) and earthworms (QBS-e) provide functional measures of soil condition, however their combined application remains largely unexplored. In this study, for the first time both indices were applied simultaneously to assess soil biological quality in the same agroecosystem. The context was that of contrasting agricultural systems (strip cropping vs. pure stands) in a real-farm experimental set-up. Additional biological variables and soil physico-chemical parameters were also considered. Statistical analyses included Spearman correlations, linear mixed-effect models (LMMs), and redundancy analysis (RDA). QBS-ar and QBS-e showed contrasting responses to management systems, with higher QBS-ar values under strip cropping and higher QBS-e values in pure stands. No significant relationship was observed between the two indices. Multivariate analyses indicated that both indices were significantly influenced by management and environmental variables, although with different patterns of association. QBS-ar appeared more responsive to variation in topsoil conditions, whereas QBS-e was associated with broader soil properties and seasonal community dynamics. These results indicate that QBS-ar and QBS-e capture complementary aspects of soil biological quality and should not be used interchangeably. Their combined use improves the interpretation of soil biological responses to agricultural management and associated environmental pressures. Overall, this study highlights the potential of soil fauna-based indices as practical tools for biomonitoring in agroecosystems and supports further exploration on the comparative responses of biological indexes. Full article

This study presents a high-resolution, multi-proxy palaeoenvironmental reconstruction of the Tövises fen at Pocsaj, Hungary, utilising lithostratigraphical, geochemical, malacological, and palynological analyses supported by radiocarbon dating. The sedimentary sequence documents the transition from a Late Glacial fluvial system (c. 19,000–16,000 cal BP) to a cut-off meander and subsequent oxbow lake, eventually evolving into a peat-forming fen. Malacological and palynological data reveal the co-occurrence of cold-tolerant Late Pleistocene elements and the early appearance of thermomesophilous taxa at the onset of the Holocene. This suggests that the favourable microclimate of the adjacent loess-covered high bank and the humid alluvial plain functioned as a cryptic refugium for temperate broad-leaved trees and associated fauna during the Late Glacial. Anthropogenic impact is traceable from the Mesolithic, characterised by Corylus management, intensifying through Neolithic agriculture to a peak during the Roman Imperial Period. Geochemical markers in the upper peat sequence reflect increased biomass and medieval habitation, while recent malacofaunal shifts indicate progressive desiccation. Despite modern drainage attempts, the Tövises fen remains a biodiversity hotspot of high conservation value, preserving relict wetland communities. Full article

Intensive pesticide application sustains global agriculture but poses poorly characterized risks to complex soil ecosystems. Here, we quantitatively evaluated pesticide residues and utilized high-resolution environmental DNA (eDNA) metagenomics to decode multi-trophic community responses across a typical major grain-producing region located in China. Among 39 targeted pesticides, 26 were detected with total concentrations ranging from 27.9 to 478.8 ng/g. While herbicides and fungicides dominated the residual mass, insecticides posed the most severe ecological threat. Notably, the neonicotinoid imidacloprid exhibited high-risk levels (RQ = 1.78 ± 1.49) at >61.1% of the sampling sites. eDNA profiling and Procrustes analyses revealed a clear trophic-dependent sensitivity gradient (p < 0.01). Lower-trophic microbial communities were significantly altered in composition; pesticide stress was strongly associated with profound non-target suppression on keystone plant-beneficial bacteria (e.g., Nocardioides). Concurrently, the fungal eDNA profiles indicated that the soil mycobiome harbored an alarming 34.7% of potential phytopathogenic fungi (e.g., Aspergillus and Colletotrichum), intrinsically driving the massive fungicide reliance. In contrast, higher-trophic soil metazoa (Rotifera, 40.4%) and weed communities (e.g., Digitaria sanguinalis) exhibited significant spatial stability, reflecting robust environmental buffering and herbicide-driven ecological escapes. Furthermore, co-occurrence networks decoupled target from non-target toxicities, uniquely revealing that persistent herbicide metabolites (desethylatrazine) induce prolonged legacy toxicities on specific soil fauna. Collectively, this study unveils the deep, cross-kingdom ecological disruptions caused by current pesticide regimes, underscoring the urgency of integrating eDNA biomonitoring to guide precision pest management and safeguard soil health in vital agricultural hubs. Full article

The Anatolian Diagonal is a major mountain system in Türkiye, yet its community-level structuring effect on mammalian assemblages remains understudied. This study provides a comprehensive inventory of the mammal fauna in Ağrı Province and evaluates the biogeographical role of the Diagonal by comparing 14 provinces. Field surveys in Ağrı (2014–2015) across 228 localities identified 16 medium- and large-sized mammal species (705 individuals), including Felis silvestris as a new provincial record. Community structure followed a lognormal distribution (AIC: 95.29). Regional patterns were analysed using presence–absence data on medium- and large-sized mammals using alpha and beta diversity indices, PERMANOVA, and Partial Canonical Correspondence Analysis (PCCA). PERMANOVA revealed significant differences in mammal assemblages between the western and eastern sides of the Diagonal (F = 3.08, R² = 0.19, p = 0.007). Beta diversity was driven predominantly by species turnover (83.8%) rather than by nestedness (16.2%). Notably, PCCA confirmed that biogeographical position significantly influenced community variation independently of habitat, elevation, and slope. These findings support a biogeographical filtering effect associated with the Anatolian Diagonal, where species replacement drives diversity patterns. The results emphasize the necessity of trans-regional conservation strategies that account for the distinct faunal compositions across this evolutionary boundary. Full article

The distribution of medically significant ticks in Vietnam requires ongoing monitoring. This study presents data on tick distribution and molecular screening for Borrelia spp. and Coxiella burnetii DNA. Ticks were collected from domestic animals and vegetation in four provinces over the period of 2024–2025. Species identification was performed based on morphology and confirmed by sequencing mitochondrial COI and 16S rRNA genes. A total of 2347 ticks were collected, representing eight species from the genera Haemaphysalis, Rhipicephalus, and Amblyomma. The study provides new distribution records for H. bispinosa, H. cornigera, A. integrum, and several rarely reported species (H. lagrangei, H. hystricus, and H. wellingtoni). PCR screening revealed a relatively high detection rate of Borrelia DNA in H. cornigera from Cao Bang province. Sequencing identified the pathogen as B. theileri, the agent of bovine borreliosis. Borrelia theileri was also detected in R. microplus in other regions, indicating wider circulation. PCR screening for Coxiella burnetii was positive for 13 ticks from cattle in Cao Bang province. To rule out false-positive results due to detection of DNA from Coxiella-like endosymbionts, we sequenced a fragment of the IS1111 element for three positive samples. The sequences confirmed that the DNA belongs to bacteria of the genus Coxiella, but the data do not allow confident assignment to C. burnetii at the species level. These positive ticks originated from eight neighboring households, suggesting a potential localized focus that requires further assessment in livestock and humans to determine the epidemiological significance. This research enhances the understanding of Vietnam’s tick fauna and associated pathogens of medical and veterinary importance. Full article

This article addresses a pressing issue—the study of ecological and geodynamic conditions in the southern part of the Uzbekistan Republic of Karakalpakstan Ustyurt plateau. The article synthesizes and systematizes the findings from both archival and personal research on the current state of ecological and geodynamic conditions. A schematic map of ecological–geodynamic conditions has been developed to assess the manifestation of various processes and their impact on the region’s flora and fauna, as well as on engineering and geological conditions relevant to mineral development and human economic activities. The overarching methodology involves system analysis and mapping of the natural-geological environment and geodynamically active zones. The primary criteria for evaluation include the state of the landscape, soil-grounds, and soil-forming rocks. The ecosystem serves as a nutrient base for plants and a fodder base for livestock development. In the Karakalpak Ustyurt, precipitation distribution is uneven, with annual precipitation ranging from 200–220 mm in the central and northern parts to 120–140 mm in the southern part. The Karakalpak Ustyurt presents morphological challenges related to the study of the nature of the relief, surface runoff, and the composition, state, and properties of the soil massif, including its ecological and geodynamic indicators. This study aims to provide a comprehensive understanding of the region’s ecological dynamics, contributing to sustainable development and conservation efforts. Thus, the conducted studies have revealed geodynamic processes associated with both natural geological phenomena and human engineering or economic activities. In principle, ecological geodynamics and engineering geodynamics rely on the same geological information to assess the manifestation of these processes Full article

Spain is the world’s leading producer and exporter of olive oil, with Andalusia being the autonomous community with the largest cultivated area. In recent decades, agricultural practices have followed a trend toward maximizing production without adequately considering ecosystem contamination. Olive groves are, in fact, complex agroecosystems in which thousands of plant species and numerous plant communities have been documented, supporting a rich diversity of vertebrate and invertebrate fauna. Intensive and unsustainable practices have led to a decline in floral diversity and, consequently, in faunal diversity. The aim of this research is to demonstrate the loss of floristic diversity associated with herbicide use. To this end, a comparative analysis of floristic diversity was conducted across 117 plots, contrasting data collected in 2007 with that from the same plots in 2021. From a methodological perspective, an inquiry-based approach was implemented involving students from the Master’s program in Olive Growing, Olive Oil, and Health. Abundance–dominance indices were compared, and the Importance Value Index (IVI) was calculated to assess changes in plant community composition. For instance, Hordeum leporinum exhibited an IVI > 70 in 2007, decreasing in 2021 to values ranging between 11 and 31.58. Similarly, Sinapis alba subsp. mairei showed a decline in IVI from 81.06 to 26.35. A notable result is the greater floristic change observed in plots located on basic substrates where herbicides were applied, compared to plots on siliceous substrates designated for grazing. This issue clearly highlights a lack of knowledge regarding appropriate cultivation techniques that promote sustainable development and social awareness. It underscores the need for educational interventions that foster learning at all levels about agricultural practices, sustainability, and ecosystem services. Full article

Methane (CH₄) is the second most important greenhouse gas after carbon dioxide (CO₂), and its atmospheric concentration is on the rise. Soil CH₄ consumption (=absorption) capacity is declining due to reduced forests and green spaces, as well as other environmental factors and anaerobic stresses. Environmental and stand structure parameters were cross-referenced with publicly available international ecosystem databases, such as FLUXNET, ICOS, NEON, AmeriFlux, the TRY plant trait database and the Oak Ridge FACE site. Searches were conducted using keywords such as region, water level, and stand density. The data indicate that under high-CO₂ conditions, the increase of forest canopy density leads to increased litter accumulation on the forest floor and reduced sunlight penetration, creating anaerobic conditions. This can cause forests to shift from CH₄ consumption to CH₄ release. Based on these findings, we discussed methods to maintain and enhance the CH₄-absorbing capacity of forest soils. This can be achieved through management practices that improve environmental conditions and increase soil fauna’s activity, such as those associated with thinning operations in overmature forest stands across various regions. This ecological manipulation through thinning practices promotes ground-level temperature increases and the activities of soil fauna, as well as maintaining aerobic conditions near the soil surface. Full article

The Rio Grande Rise (RGR) is the largest oceanic plateau in the South Atlantic and represents a key natural laboratory for understanding oceanic plateau formation, deep-sea circulation, ecosystem functioning, and ferromanganese crust development. This study presents a critical synthesis of current scientific knowledge on the RGR, integrating geological, geophysical, oceanographic, biological, and geochemical evidence published over the last two decades. Geophysical data reveal a complex tectono-magmatic evolution involving Late Cretaceous plume-related volcanism, crustal thickening, rifting, and subsequent subsidence. The structural framework of the plateau is dominated by the Cruzeiro do Sul Rift, which plays a central role in controlling sedimentation, magmatism, and seawater circulation. Oceanographic studies demonstrate that the interaction between the southern branch of the South Equatorial Current and the complex topography of the RGR generates intense internal tides and bottom currents, strongly influencing sediment transport and benthic habitats. Biological investigations indicate that the RGR hosts diverse deep-sea communities, including sponge grounds, cold-water corals, and associated fauna, whose distribution is tightly linked to geomorphology and hydrodynamics. Ferromanganese crusts occurring on the plateau preserve valuable geochemical records of oceanographic and redox conditions, although their spatial distribution, thickness, and metal budgets remain incompletely constrained. Despite major advances, significant knowledge gaps persist regarding crustal structure, sedimentary evolution, ecosystem functioning, and mineral formation processes. This review highlights these uncertainties and outlines research priorities necessary to improve understanding of oceanic plateaus and deep-sea systems in the South Atlantic. Full article

Renewable energy sources, and wind energy in particular, constitute a central pillar of energy policy at both national and European levels. Nevertheless, the deployment of onshore wind farms is frequently associated with spatial, environmental, and social conflicts, making the evaluation of existing projects imperative. The present study aimed to assess the sustainability of existing onshore wind farms in the Region of Thessaly, with particular emphasis on their spatial planning, technical characteristics, and environmental impacts. The methodological framework consists of four distinct stages: (i) identification and spatial mapping of existing wind farms in the study area, (ii) assessment of the compliance of existing wind installations with the Specific Framework for Spatial Planning and Sustainable Development for Renewable Energy Sources (SFSPSD–RES), (iii) application of the Rapid Impact Assessment Matrix (RIAM) to enable a systematic and comparable evaluation of the impacts of wind installations on specific environmental and anthropogenic parameters, and (iv) estimation of project hazard and operational vulnerability through the application of Operational Risk Management (ORM). Geographic Information Systems (GISs) were employed for data processing and spatial analysis. The assessment showed that 40% of the evaluated wind farms fully comply with all eleven exclusion criteria of the SFSPSD-RES, whereas the remaining 60% show partial compliance, failing to meet between one and three criteria. RIAM results indicate that the most significant adverse impacts (−D and −C) during construction are associated with morphology/soils and the natural environment, mainly due to loss/fragmentation of vegetation and disturbance of fauna, and, in some cases, in areas of increased sensitivity. During operation, the main negative effects (−D and −C) relate to landscape and visual quality, as well as continued disturbance to the natural environment. At the same time, the operation generates important positive effects (+E) on the atmospheric environment through reduced CO₂ emissions. The ORM analysis further shows that the most important risks for most wind farms arise during construction (ORM = 2 and 3), particularly from serious worker accidents during lifting, roadworks, and foundation activities. The study demonstrates that the sustainability of existing wind installations depends on a complex set of spatial, environmental, and technical factors. The proposed framework integrates spatial compliance screening, RIAM-based environmental impact assessment, and ORM-based risk and opportunity evaluation. This connection links the importance of impacts with their operational manageability during construction and operation phases, as well as across sustainability dimensions. Consequently, the study provides a more decision-focused approach for assessing existing wind farms and supporting policy development. Full article

Anostomidae is one of the most diverse families within the order Characiformes and is widely distributed across the river basins of the Neotropical region. Anostomids are known to host a rich diversity of metazoan parasites, including ectoparasites, such as monopisthocotylans, and endoparasites, such as nematodes, cestodes, and digeneans. The present study aims to develop an inventory of metazoan parasites recorded in fish of the Anostomidae family. A bibliographic review of metazoan parasite species in anostomids was conducted, using searches in different databases. This bibliographic review was complemented with information from books and book chapters on ictioparasitology. The data were compiled until January 2025. Based on the review, 191 taxa (126 are described at the species level and 65 were identified only to the genus level or higher taxonomic categories) of metazoan parasites associated with anostomid fishes were found. Monopisthocotyla and Nematoda were the taxonomic groups with the most parasitic associations at the species level, with 31 and 30 species, respectively. This study presents a comprehensive and updated inventory of metazoan parasites associated with fish from the family Anostomidae, covering over 170 years of research (1850–2025). This survey highlights the importance of anostomids as hosts for a rich parasitic fauna. Full article

Erosion in intensively farmed landscapes threatens above- and below-ground biodiversity. While impacts on soil physical and chemical properties (which affect soil inhabiting biota) are well documented, effects on ground-associated fauna (distribution, diversity, abundance) remain less understood. A likely very strong factor is the direct transport of epigeon together with the eroded soil. We assessed how water-erosion processes shape communities of epigeic invertebrates along agricultural slopes in the Chernozem region of South Moravia (Czech Republic). Ground-dwelling invertebrates were sampled over five years (May–September) in conventionally managed maize fields using pitfall traps across 18 sloping fields. Three slope positions were compared per field (control, erosional, depositional; 54 positions in total). Community patterns were evaluated using Canonical Correspondence Analysis with covariates (month, year, slope position, site), and species responses to key drivers were analysed using Generalised Additive Models. Across the full dataset, Shannon diversity and species richness did not differ significantly among slope positions; however, total invertebrate abundance was significantly lower in erosional parts. Interannual variation was pronounced and linked to precipitation: wet conditions increased diversity and richness at depositional positions, whereas dry conditions reduced diversity downslope. Ordination and GAM results identified erosion intensity and relative precipitation/temperature anomalies as important predictors, with most dominant species showing higher abundances under low to moderate erosion. These findings indicate that epigeic invertebrate communities along slopes can serve as indicators of erosion force. Full article