Search Results (491)

Plant-mediated interactions can modify herbivore behaviour and vector-borne plant disease dynamics, yet the systemic consequences of prior cereal leaf beetle (Oulema spp.) window-pane feeding for later phloem feeder vectors remain unclear. We hypothesised that prior Oulema window-pane feeding induces systemic changes in barley that alter the subsequent host acceptance and feeding behaviour of Psammotettix alienus. Plants received one of three 24 h treatments on one leaf: Oulema pre-colonisation, standardised artificial vascular injury or an undamaged control. Leafhopper responses were measured on a different, systemic leaf using female choice assays and electrical penetration graph recordings. Oulema damage was low and strongly right-skewed and did not affect most feeding variables, including non-phloem activities, phloem activity, drinking latency or phloem activity latency. Artificial vascular injury, however, reduced the phloem–saliva ratio in females, which indicates a lower relative investment in phloem preparation once the phloem phase was reached. Within Oulema-treated plants, increasing damage reduced the number of transitions between penetration phases in males but not females. Females showed no treatment-related preference in salivary sheath production or oviposition. Thus, Oulema window-pane feeding left only a weak systemic plant-mediated legacy for P. alienus, closer to behavioural neutrality than strong facilitation or interference under these short-term systemic experimental conditions. Full article

Background/Objectives: This study aimed to evaluate the direct effect of elements released from dental casting alloys on human gingival fibroblasts (HGF). Methods: Ten discs each of commercially pure titanium (Ti), silver-palladium (Ag-Pd), and nickel-chromium (Ni-Cr) alloys, measuring 4 mm in diameter and 3 mm in thickness, were fabricated and placed directly on HGF cultures for 72 h. Five discs from each alloy group were incubated in DMEM, whereas the remaining five discs were incubated in artificial saliva. Cytotoxicity was assessed using three in vitro methods: morphological evaluation of HGF, the dye exclusion test (DET), and the methyl-thiazolyl-tetrazolium (MTT) colorimetric assay. Results: HGF directly exposed to cpTi, Ag-Pd, and Ni-Cr discs demonstrated reduced cell density around the alloy specimens compared with untreated control cells after 72 h of incubation in both DMEM and artificial saliva. cpTi alloy exhibited no detectable cytotoxic effects, whereas Ag-Pd and Ni-Cr alloys demonstrated cytotoxicity in both DMEM and artificial saliva under the experimental conditions. Among the tested alloys, Ni-Cr exhibited the highest cytotoxicity. Artificial saliva enhanced the cytotoxic effects of the tested alloys, as evidenced by increased morphological alterations, reduced cell viability, and lower MTT activity compared with DMEM. Conclusions: cpTi alloy exhibited the lowest direct cytotoxic effect on HGF in both DMEM and artificial saliva. In contrast, Ag-Pd and Ni-Cr alloys demonstrated greater cytotoxicity, particularly in the presence of artificial saliva, suggesting that both alloy composition and the surrounding environment influence the biological response of oral soft tissues. Full article

Tooth avulsion is one of the most severe dental injuries, and appropriate storage media are essential for preserving avulsed teeth before replantation. However, little is known about the effects of these media on the color stability of existing restorative materials. This in vitro study evaluated the color stability of a composite resin and a glass hybrid restorative material immersed in six storage media commonly recommended for avulsed teeth. A total of 120 disc-shaped specimens were prepared and allocated to dry environment, artificial saliva, tap water, cow’s milk, goat milk, and kefir groups. Color measurements were performed using the CIE L*a*b* system at baseline, 30 min, 1 h, 6 h, 12 h, and 24 h, and color change (ΔE) values were calculated. Data were analyzed using a linear mixed-effects model with restorative material, storage medium, and time as fixed effects and specimen ID as a random effect. Color change increased significantly over time in all groups (p < 0.001). Glass hybrid restorations exhibited significantly higher ΔE values than composite restorations across most storage media and time points (p < 0.001). Significant material–medium–time interactions were observed (p < 0.001), indicating that the effects of storage media varied according to restorative material and exposure duration. Dry storage conditions were generally associated with the highest color changes. Within the limitations of this study, the tested composite restorative material (G-ænial Posterior) demonstrated greater short-term color stability than the tested glass hybrid restorative material (EQUIA Forte HT) in the evaluated storage media. Full article

Background and Objectives: In-office bleaching is one of the most requested aesthetic procedures, but its biomechanical aftermath on enamel—particularly the recovery trajectory of microhardness, surface roughness, and erosive vulnerability—remains incompletely characterised. The present study quantified the 28-day mechanical recovery profile of enamel after three commonly used chairside bleaching protocols and examined inter-relationships between mechanical, topographic and erosive endpoints. Methods: Forty-two human molars and premolars (27 molars, 15 premolars) extracted for clinical reasons and previously catalogued in the institutional biobank were sectioned along the cervical–occlusal axis to obtain matched control and experimental halves. The experimental halves were assigned (n = 14 per group) to Opalescence Quick (45% carbamide peroxide, no light), Opalescence Boost (40% hydrogen peroxide, chemically activated), or BlancOne Ultra+ (35% hydrogen peroxide with light activation). Vickers microhardness (VHN), profilometric roughness (Ra), and erosive wear after a standardised citric-acid challenge were measured at baseline, immediately post-bleaching, and at 24 h, 7 d, 14 d, and 28 d in artificial saliva. Wilcoxon signed-rank, Kruskal–Wallis with Dunn–Bonferroni post hoc, linear mixed-effects models, multiple linear regression, ROC analysis, and Spearman correlations were used (α = 0.05). Results: Immediate VHN drops were −13.2 ± 3.9%, −22.6 ± 3.6%, and −29.6 ± 4.7% for Opalescence Quick, Opalescence Boost, and BlancOne Ultra+, respectively. By 28 days, recovery reached 97.4 ± 2.2%, 96.0 ± 2.9%, and 91.6 ± 2.3% of baseline (p < 0.001). Exponential rate constants were 0.137, 0.075, and 0.092 d⁻¹. Erosive wear after acid challenge was 2.1×, 2.8×, and 3.8× control values. ROC analysis identified Δ-immediate Ra as the strongest predictor of incomplete recovery (AUC = 0.859). Conclusions: Higher-aggression protocols delayed mechanical recovery and amplified erosive susceptibility, with light-activated systems carrying the greatest residual risk. These in vitro findings provide an evidence base for the design of future clinical studies on post-bleaching enamel recovery and remineralisation. Full article

Background: Patients with White spot lesions (WSLs) are seeking minimally invasive remineralizing treatment. While fluoride is the gold standard of non-invasive caries therapy, its remineralizing impact is predominantly superficial. MI Paste One (MIP-1) combines casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) with 1100 ppm fluoride. This in vitro study aimed to assess the effect of MIP-1 compared with other agents. Methods: Enamel slabs were demineralized to create a caries-like lesion and randomized into five groups (n = 10): G1, WSL/artificial saliva (AS) (negative control); G2, deionized water brushing; G3, fluoridated toothpaste brushing; G4, MIP-1 brushing; G5, topical MI Paste application. Color change (ΔE), gloss (GU), microhardness (VHN), roughness (Ra), and surface topography (SEM) were assessed at four time points: baseline, first demineralization, treatment, and second demineralization. Results: All active agents improved microhardness after treatment. After secondary demineralization, G4 achieved the highest hardness, significantly superior to G3 (p = 0.016). G5 occupied a statistically intermediate position. CPP–ACP-containing agents (G4, G5) achieved color improvement that was significantly superior to controls. Conclusions: MIP-1 outperformed fluoride toothpaste in resisting secondary demineralization of permanent enamel WSLs. CPP–ACP made a measurable independent contribution to demineralization resistance in permanent enamel. These findings highlight the need for further clinical investigation of MIP-1. Full article

This study investigates the effects of post-processing on the surface roughness, wettability, and frictional behavior of selective laser-melted (SLM) cobalt–chromium–tungsten (CoCrW) alloys for orthodontic use. The SLM-CoCrW specimens were tested in as-manufactured, mechanically polished, and electropolished states. Surface characterization via stylus profilometry and atomic force microscopy (AFM) showed that both polishing methods reduced macro- and micro-scale roughness, with electropolishing producing the smoothest, most uniform topography. Static water contact angle (WCA) measurements revealed that mechanical polishing provided an optimal balance of roughness and hydrophilicity, resulting in the lowest friction, while ultrasmooth electropolished surfaces exhibited slightly higher friction due to increased hydrophobicity and a uniform Cr-rich oxide layer confirmed by X-ray photoelectron spectroscopy (XPS). XPS also indicated that electropolishing generated a homogenous chromium oxide passive film, whereas mechanical polishing left a chemically heterogeneous surface with exposed metallic sites. Importantly, performance is not governed solely by surface roughness; surface chemistry is equally critical, and both must be considered together—along with wettability and tribological behavior—to achieve optimal functional outcomes. From a clinical perspective, optimization of surface roughness and surface chemistry may result in decreased frictional resistance, improved sliding mechanics, and enhanced long-term performance of additively manufactured orthodontic components; however, the present study was restricted to in vitro characterization under simplified laboratory conditions, and further investigations involving artificial saliva, long-term aging, wear and clinical simulations are necessary to validate the translational relevance of these findings. Full article

Background/Objectives: Oral cavity squamous cell carcinoma (OSCC) remains a major global health burden, with outcomes strongly dependent on stage at diagnosis. Although the oral cavity is directly accessible to clinical examination, many cases are still detected at advanced stages. This narrative review aimed to summarize current trends in OSCC diagnosis and early monitoring, with emphasis on non-invasive and minimally invasive techniques and biomarkers. Methods: A semi-systematic narrative literature search was conducted in PubMed/MEDLINE, Scopus, and Web of Science using predefined combinations of OSCC-, early-detection-, imaging-, cytology-, liquid-biopsy-, salivaomics-, artificial-intelligence-, and biosensor-related terms. English-language systematic reviews, meta-analyses, reviews of reviews, translational studies, and clinically relevant original articles were prioritized, with explicit attention to oral cavity-specific evidence and clearly identified extrapolation from broader head-and-neck or oropharyngeal cancer settings. Results: Conventional oral examination and histopathological assessment of biopsy specimens remain the diagnostic foundation. Adjunctive methods may support lesion triage, biopsy-site selection, risk stratification, and early monitoring, but cannot replace tissue diagnosis. Narrow-band imaging, optical coherence tomography, and molecular brush cytology appear particularly promising for specialist assessment and surveillance. Liquid biopsy and saliva-based biomarker platforms offer translational potential, particularly for repeatable monitoring, but clinical implementation is limited by methodological heterogeneity, pre-analytical variability, inconsistent thresholds, and insufficient external validation. Artificial intelligence and biosensor platforms remain promising but largely developmental. Conclusions: Progress in early OSCC diagnosis and monitoring will most likely depend on integrated diagnostic models combining clinical examination, adjunctive imaging, minimally invasive sampling, molecular biomarkers, and computational decision-support tools, validated in prospective multicenter studies. Full article

Background: Compomer materials combine the advantages of composite resins and glass ionomer cements, including fluoride release, durability, and aesthetics. This study evaluated the effects of silver nanoparticles (nAg⁰) and copper oxide (CuO) particles on fluoride ions release and the structural properties of a commercially available compomer. Methods: Compomer discs modified with 0.125 wt.%, 0.25 wt.%, and 0.5 wt.% nAg⁰ or CuO were prepared and analyzed in demineralized water and artificial saliva at various pH levels for 168 h. Fluoride release was measured using a fluoride-selective electrode, while structural and morphological properties were examined using X-ray diffraction (XRD) and scanning electron microscopy (SEM). Results: Under most of the tested conditions, the modified materials exhibited higher fluoride release than the unmodified compomer, with the greatest increase typically observed at higher additive concentrations. XRD analysis confirmed the presence of crystalline phases of Ag⁰ and CuO while maintaining the amorphous nature of the compomer matrix. SEM observations revealed better particle dispersion at lower additive concentrations and increased agglomeration at a 0.5% content. Conclusions: These results indicate that the incorporation of nAg⁰ and CuO particles may enhance the fluoride-releasing potential of compomer materials; however, further studies are necessary to evaluate their mechanical, antibacterial, cytotoxic, and aesthetic properties prior to clinical application. Full article

Surface-enhanced Raman scattering (SERS) offers a powerful route for biomolecular detection because it combines molecular specificity with high sensitivity, rapid optical readout, and multiplexing capability. In real biological samples, however, analytical performance is rarely determined by signal enhancement alone. Biofluids such as serum, plasma, saliva, urine, and interstitial fluid contain complex biomolecular mixtures that interfere with target capture, spectral response, and data interpretation. A practical SERS biosensor must therefore localize targets, stabilize spectral responses, tolerate matrix-induced variation, and convert complex spectra into reliable analytical information. This review discusses recent progress in SERS biosensing from an integrated system perspective, with particular focus on artificial intelligence/machine learning (AI/ML)-assisted interpretation. Direct label-free SERS provides chemically transparent readouts but is limited by stochastic adsorption, hotspot heterogeneity, and spectral variation in complex samples. Bio-recognition interfaces improve target localization, while signal-transduction strategies based on nanotags, immunoassays, clustered regularly interspaced short palindromic repeats (CRISPR) systems, nanozymes, and lateral-flow formats decouple molecular recognition from spectral generation. Digital SERS further improves measurement robustness by converting fluctuating intensities into countable, event-based outputs. AI/ML-assisted analysis can support full-spectrum classification, calibration transfer, explainability, and patient-level decision-making. We frame AI/ML-assisted SERS biosensing as an integrated architecture connecting substrate design, interface engineering, signal transduction, digital measurement, and clinical validation. Future progress will depend as much on validation-ready workflows as on plasmonic enhancement itself, especially for systems intended to operate across different samples, instruments, and clinical settings. Full article

The optical stability and aesthetic performance of dental polymers are critical for the longevity of restorative treatments. This study aims to evaluate the influence of different polymerisation conditions and maintenance protocols—specifically the use of charcoal pastes and brushes—on the Whiteness Index for Dentistry (WID) of polymer samples. Four groups of polymer samples (Optiprint Lumina, Dentona, AG) were prepared under various conditions: Group I (Standard: 7 min, 22 °C), Group II (Thermal: 7 min, 60 °C), Group III (Extended: 20 min, 22 °C), and Group IV (Glycerin barrier: 7 min, 22 °C). The protocol consisted of brushing with standard or carbon-infused toothpastes and brushes for a period of 2 min. Between brushing, the samples were stored in artificial saliva at 37 °C to simulate the oral environment. The samples were divided into groups. The first group was brushed with a standard paste and a standard brush; the second group was brushed with a simple paste and a carbon brush; the third group was brushed with carbon paste and a standard brush; and the fourth group was brushed with carbon paste and a carbon brush. Changes in the Whiteness Index for Dentistry (WID) were recorded and analysed statistically using a paired t-test and Pearson correlation. Results: All groups showed a statistically significant increase in the WID (p = 0.0191). Group IV (glycerin barrier + carbon paste/brush) exhibited the highest increase, with a WID = 2.0, demonstrating a synergistic effect between oxygen inhibition control and activated charcoal. In contrast, Group II (thermal polymerisation) showed the highest chromatic stability (ΔWID = 0.6), remaining below the Whiteness Perceptibility Threshold (WPT = 0.72). No significant correlation was found between polymerisation time and WID changes (r = 0.087, p = 0.913), indicating that temperature and surface treatment are the primary drivers of optical modification. Conclusions: The heat parameter did not reveal a significant difference in the optical properties. Furthermore, the combination of a glycerin barrier and carbon-based hygiene products maximises the whitening effect while remaining within the Whiteness Acceptability Threshold (WAT < 2.60), providing valuable insights for both material processing and patient maintenance protocols. Full article

Antigen detection provides rapid and convenient diagnosis of respiratory infections. This study develops an innovative dual-fluorescence aptasensing method based on polydopamine-functionalized MXene (PDA-MXene) for the simultaneous detection of spike protein and hemagglutinin protein. The method employs green- and red-emitting quantum dot (QD) probes as fluorescence reporters, and the PDA-MXene as an effective adsorption and separation substrate. Coupled with a centrifugation-assisted separation strategy, this design method reduces background interference and enhances detection reliability. The method demonstrates good analytical performance, with detection limits of 0.82 ng/mL for spike protein and 2.11 ng/mL for hemagglutinin protein in single-channel mode. The dual-channel mode enables reliable and simultaneous quantification of both target proteins with minimal spectral cross-talk. Furthermore, this method exhibits high specificity against interferents including ions, proteins, and toxins. Artificial saliva, chosen as real sample, is spiked with target proteins to investigate the practical applicability of the method, showing recovery rates for both target proteins between 100 and 114 sensing strategy is simple to operate and allows the detection of new targets by simply replacing the azide-modified aptamer lyophilized powder. It therefore holds promising application for the simultaneous detection of multiple proteins in point-of-care testing and health monitoring fields. Full article

Background: Bisphenol A (BPA) release from resin-based dental materials is a growing concern due to its potential endocrine-disrupting effects, particularly in pediatric patients. This in vitro study evaluated cumulative BPA release and elution kinetics from commonly used pediatric resin-based materials, due to the limited evidence available. Methods: Three restorative materials (Clearfil Majesty ES-2, Estelite Sigma Quick, and Stela Automix) and one orthodontic material (Transbond XT) were investigated. Eighteen disk-shaped specimens (5.5 mm in diameter and 2 mm in thickness) were prepared for each material and immersed in artificial saliva (pH 6.8) at 37 °C for 1, 7, and 28 days. BPA concentrations were quantified using liquid chromatography–tandem mass spectrometry (LC–MS/MS). BPA release kinetics were evaluated during the early (1–7 days) and late (7–28 days) release phases. Results: All investigated materials released measurable BPA concentrations, with cumulative BPA release progressively increasing up to 28 days. Clearfil Majesty ES-2 and Estelite Sigma Quick exhibited the highest cumulative BPA concentrations, whereas Stela Automix showed markedly lower values. Transbond XT also demonstrated measurable BPA release. For all materials, BPA release kinetics were significantly higher during the early phase than during the late phase (p < 0.001), indicating a non-linear release behavior over time. Conclusions: BPA release from pediatric restorative and orthodontic resin-based materials is material-dependent and characterized by progressive cumulative accumulation associated with significantly higher early-phase release rates. These findings highlight the importance of assessing the safety of resin-based materials used in pediatric dentistry. Full article

The corrosion phenomenon can cause negative allergic and cytotoxic reactions in the human body, inflammation, and, in the future, the development of cancer. Their sources may be corrosion products, metal ions released during the corrosion process, and galvanic currents that penetrate the surrounding tissues. In order to avoid the negative effects of using metal alloys, their surface can be modified by applying coatings. The aim of this study is to determine and compare the amount of ion release from Si(C,N) coatings with varying carbon and nitrogen contents, as well as from the uncoated substrate alloy (Group A) in various aqueous environments. Si(C,N) coatings were applied to the surface of the prosthetic alloy. Si(C,N) coatings with different carbon and nitrogen contents were deposited using the reactive magnetron sputtering (RMS) method. The research included determining the amount of ions released into the environment: distilled water, 0.9% NaCl and artificial saliva. Assessments were made at 10, 30 and 90 days. All tested Si(C,N) coatings significantly limit the amount of metal ions in the surrounding medium. Due to the lack of statistically significant differences in the number of ions released by individual coatings, when selecting them, other properties related to the operating conditions of the elements should also be taken into account. Full article

The aim of this study was to evaluate the influence of artificial saliva ageing and cyclic hydrothermal loading on the mechanical properties of dental composite materials. Two commercial composites (Filtek Z550 and Filtek Ultimate Flow) and two experimental materials representing flow-type and hybrid composites were investigated. SENB specimens were prepared in accordance with ASTM E399, together with flat specimens intended for impact strength testing using the Dynstat method. All samples were aged in artificial saliva for approximately one month at 37 ± 1 °C, and subsequently, half of the specimens were subjected to thermocycling in the temperature range of 10–65 °C for 10,000 cycles. Static mechanical tests, including three-point bending (TFS), biaxial flexural strength (BFS), and compression strength (CS), were performed before and after thermocycling. In addition, impact strength and fracture toughness expressed by the stress intensity factor K_IC were determined. The results were analyzed in terms of the residual work of fracture (WOF), while the durability of the materials was evaluated using Weibull distribution parameters. The experimental analysis was complemented by SEM observations of the microstructure. The obtained results demonstrated a pronounced deterioration of mechanical properties after hydrothermal loading. The average impact strength after artificial saliva ageing reached 11.69 J/mm² for Filtek Z550, 11.57 J/mm² for Ex-hyb(P), 16.39 J/mm² for Filtek Ultimate Flow, and 10.27 J/mm² for Ex-flow(P), whereas after thermocycling, these values decreased to 5.38 J/mm², 8.86 J/mm², 4.55 J/mm², and 4.39 J/mm², respectively. A similar trend was observed for the fracture toughness parameter KIC, which decreased considerably after thermocycling for all investigated materials. The analysis of the residual work of fracture revealed the influence of thermocycling on the energy-related parameters of the composites. In the case of TFS, the average WOF decreased, among others, from 13.65·10⁻³ J to 1.90·10⁻³ J for Filtek Ultimate Flow and from 4.76·10⁻³ J to 2.37·10⁻³ J for Filtek Z550. For BFS, a noticeable decrease in WOF was also observed, particularly for Ex-flow(P) and Filtek Ultimate Flow. In the compression tests (CS), the changes were less unambiguous, and some materials exhibited an increase in WOF after thermocycling. Furthermore, changes in the scale and shape parameters of the Weibull distribution were identified, indicating degradation of composite durability under hydrothermal loading. The results confirmed that cyclic hydrothermal loading exerts a greater influence on impact strength and fracture toughness than on static flexural strength. While all investigated materials exhibited degradation, the extent of changes was material-dependent, and compression behaviour showed non-uniform responses. Weibull analysis confirmed reduced reliability and increased heterogeneity of the composites after ageing, indicating that hydrothermal fatigue is a dominant factor governing long-term mechanical deterioration of dental resin-based composites. Full article

Chronic kidney disease (CKD) is a progressive and irreversible disorder affecting over 850 million individuals globally and is associated with significant morbidity, mortality, and healthcare burden. Conventional diagnostic approaches rely on intermittent laboratory measurements, including serum creatinine, estimated glomerular filtration rate (eGFR), and urinary albumin, which provide limited temporal resolution and fail to capture dynamic physiological changes. Recent advances in wearable biosensing technologies offer new opportunities for continuous, non-invasive monitoring of biochemical and physiological markers relevant to renal function. This review provides a comprehensive analysis of wearable biosensors for CKD monitoring, focusing on sensing mechanisms (electrochemical, optical, and field-effect transistor), biofluid interfaces (sweat, interstitial fluid, and saliva), and materials engineering strategies enabling flexible, high-performance devices. Emphasis is placed on biofluid transport dynamics, analytical performance across sampling matrices, and system-level integration with wireless communication and digital health platforms. Key challenges limiting clinical translation, including biofouling, enzymatic instability, and variability in biofluid composition, are examined—alongside emerging solutions such as antifouling interfaces, synthetic recognition elements, and multimodal sensing architectures. Finally, regulatory pathways and the role of artificial intelligence in digital nephrology are discussed. This review highlights the potential of wearable biosensors to transform CKD management through continuous monitoring, early detection, and personalized therapeutic intervention. Full article