Search Results (671)

This study evaluated the effectiveness of eDNA metabarcoding in assessing fish communities in two urban lakes (First Lake and Second Lake) in Zagreb, Croatia, following IAS removal. Water samples were collected in April and June 2024 and analyzed using MiFish primers targeting the 12S rRNA gene. The results indicated that the cleanup efforts were largely successful, as several IAS previously recorded in these lakes were not detected (Ameiurus melas, Lepomis gibbosus, and Hypophthalmichthys spp.). However, some others persisted in low relative abundances, such as grass carp (Ctenopharyngodon idella), topmouth gudgeon (Pseudorasbora parva), and prussian/crucian carp (Carassius sp.). Species composition differed between lakes, with common carp (Cyprinus carpio) dominating Maksimir First Lake, while chub (Squalius cephalus) was prevalent in Maksimir Second Lake. Unexpected eDNA signals from salmonid and exotic species suggest potential input from upstream sources, human activity, or the nearby Zoo Garden. These findings underscore the utility of eDNA metabarcoding in biodiversity monitoring and highlight the need for continuous surveillance and adaptive management strategies to ensure long-term IAS control. Full article

This contributed paper presents results from efforts by the Association of Zoos and Aquariums (AZA) to investigate the impact of integrating conservation into AZA members’ organizational cultures. Part of this work included AZA setting goals related to organizational and professional culture, strategic communication, developing communities of practice, and promoting tools and resource-sharing. Prior to implementing the majority of these steps, a baseline assessment was administered to directors plus a random sample of AZA organizations in 2020—assessing how well conservation is integrated into the institutional culture and measures of perceived conservation impact. The same sample of organizations was re-surveyed in 2023 with the intent of a three-year cycle of surveying to monitor change and identify additional ways that AZA could support and strengthen a culture of conservation within the profession. These findings will help the zoological and broader conservation community assess opportunities to integrate conservation into organizational cultures across a broad association for the purpose of achieving the mission and impact. Full article

To identify differentially abundant polyketide synthases (PKSs) and to characterize the biochemical consequences of brevetoxin biosynthesis, bottom-up, TMT-based quantitative proteomics and redox proteomics were conducted to compare two strains of the Florida red tide dinoflagellate Karenia brevis, which differ significantly in their brevetoxin content. Forty-eight PKS enzymes potentially linked to brevetoxin production were identified, with thirty-eight showing up to 16-fold higher abundance in the high-toxin strain. A pronounced shift toward a more oxidized redox state was observed in this strain’s proteome. Notably, 25 antioxidant-related proteins were significantly elevated, including alternative oxidase (AOX), which increased by 17-fold. These results elucidate the cellular consequences of toxin biosynthesis in K. brevis, offer new leads for the study of brevetoxin biosynthesis, and suggest a novel red tide mitigation approach targeting high toxin-producing strains. Full article

Food-based environmental enrichment (EE) is a valuable strategy for stimulating foraging behaviour in fish under human care, as it increases the challenge of food acquisition and encourages prolonged engagement in this activity. Curimbas (Prochilodus argenteus) and pacus (Myleus micans) are fish species for which ex situ maintenance has become an important conservation measure. In this context, providing EE is essential to ensure high welfare standards. This study aimed to assess the effects of food enrichment on the behaviour of these two endemic species from the São Francisco River basin in Brazil. Behavioural data were collected across three experimental phases, including baseline, enrichment, and post-enrichment. Slow-dissolving food items known as “acorns” were introduced during the enrichment phase. Both species exhibited a marked reduction in inactivity throughout the study. For curimbas, the enrichment phase was associated with increased foraging, elevated agonistic interactions, and greater use of specific tank areas. Among pacus, inactivity significantly declined during the enrichment period. Additionally, the presence of visitors influenced behavioural patterns, promoting foraging activity while reducing inactivity and interactions with the enrichment device. These findings reinforce the value of incorporating environmental enrichment to promote the welfare of freshwater fish in public aquariums. Full article

Imidazole dipeptides (IDPs), including anserine, carnosine, and balenine, are predominantly found in the skeletal muscles of vertebrates. Balenine is the major IDP in cetaceans. Serum amyloid A (SAA) is an acute phase protein released in response to damage or injury in various tissues, including skeletal muscles. A captive bottlenose dolphin (Tursiops truncatus) died due to rhabdomyolysis and subsequent acute kidney injury that probably originated from accidental muscle trauma. In this study, concentrations of IDPs and SAA were measured using stored serum collected from the affected dolphin with intermittent continuous damage of skeletal muscles to demonstrate the pathological relevance of these parameters and their usefulness as biomarkers for muscle damage in dolphins. The IDP concentration was measured using the high-performance liquid chromatography-ultraviolet method. The SAA concentration was measured using an enzyme-linked immunosorbent assay (ELISA) specific to dolphin SAA and a latex turbidimetric immunoassay (LTI) specific to human SAA. Herein, the IDP concentration was altered similarly to serum muscular enzymes, including creatinine kinase (CK) and aspartate aminotransferase (AST). However, IDP concentrations were elevated one day earlier than CK and AST levels at disease onset. Furthermore, IDP concentrations were similarly altered when assessed using both ELISA- and LTI-SAAs, and the change in IDP concentration coincided with that in LTI-SAA based on the statistical analysis. These data suggest that IDP concentration could detect muscle damage and injury, including necrosis and inflammation, in dolphins. Full article

There is increasing recognition of the importance of human–animal interaction (HAI) research in the assessment of animal welfare. This is partly reflected by the appearance of increasing animal–visitor interaction (AVI) publications in zoos. Early AVI publications primarily focused on primates and the impact of visitors on animals (visitor effects), with most identifying negative welfare impacts. This review aims to identify trends and changes in the key factors of AVIs (e.g., types of interactions measured; taxa studied; welfare impact). Covidence online software and PRISMA were used to screen papers and extract data. A total of 157 papers comprising 314 studies were included. AVI publications have increased in the last two decades, with a large increase in publications since 2020. Most publications have focused on visitor effects, as opposed to the impact of animals on visitors (visitor experiences). Earlier visitor effect studies mostly focused on primates, while more recent studies have primarily focused on non-primate species. Excluding studies categorized as neutral, there were more visitor effect studies with measures focused on negative than positive welfare impacts. However, the last decade and a half has seen a substantial increase in visitor effect studies measuring positive welfare impacts. These results are discussed in reference to growing changes in the types of studies, species, and outcomes measured in AVIs. In addition, we consider the future of AVI research, including a growing need for and consideration of standardized welfare assessments, the increased use of experimental control, and AVIs that equally promote visitor education and positive animal welfare. Full article

Seahorse (Hippocampus spp.) is popular in the markets of traditional Chinese medicine, aquarium, and curio. In order to protect wild stocks and still meet the market demand, China attempted the large-scale cultivation of seahorses in the early 21st century and achieved it in the 2010s. However, in recent years, two new issues have gradually emerged in Chinese seahorse cultivation. One is that the juveniles are prone to disease during diet conversion, and the other is that the reproductive performance of broodstocks is significantly reduced. With the aim to provide some measures that can alleviate these issues, in the present study, we used lined seahorse (Hippocampus erectus, a species widely cultured in China) as the experimental subject and Astragalus polysaccharides (APSs) as the immunostimulant to test whether APSs could improve the immune-health status and reproductive performance of seahorses. The measured indices for reproductive performance included ovarian lipid content, assessment time required before mating for paired male and female seahorses, mating success rate, brood size, and newborn body height. The results showed that for juveniles during diet conversion, their body weight, survival rate, plasma immunocytokine contents (interleukin-2, interferon-α, and immunoglobulin M), and alpha diversity indices (Simpson and Pielou’s-e) of the intestinal microbiota were significantly higher than those of the control group after dietary APSs. For broodstocks, compared with the control group, the expression of lipid substances in the ovary was significantly upregulated, the assessment time was significantly shortened, and the body height of their newborns was significantly increased in the APS group. These results demonstrate that APSs could indeed improve the immune-health status and reproductive performance of seahorses, providing guidance for addressing existing issues in seahorse cultivation. Full article

Across the globe, many animals with disabilities live in zoos and aquariums, yet these institutions often face difficult questions about how to share those stories with the public in a way that raises awareness and minimizes concern about the animals’ well-being. This study explored whether anthropomorphic narrative signage could help visitors form meaningful emotional connections with these animals and, in doing so, encourage greater understanding and support for people with disabilities. Drawing on ideas from contact and transfer theories, we conducted observational research in zoo settings, comparing visitor reactions to no signage, detailed signage, and simple signage. We found that simple signage resulted in fewer negative comments, fewer questions about the animal’s health, and less frustration directed at the organization. In a related survey experiment, participants who saw a photo and brief story about an animal with a disability were more likely to express empathy and hold more positive views toward both animals and people with disabilities. These findings highlight the power of accessible messaging in informal learning spaces to reduce stigma and promote inclusion. The project was led by a diverse, interdisciplinary team of zoo professionals and disability scholars, most of whom bring personal experience with physical or cognitive disabilities to the work. Full article

Marine animals without faces (MAWFs), are some of the most important creatures maintaining the ecological balance in marine environments. How these animals are depicted across conservation organizations may impact public perceptions and conservation efforts. We assessed the online presentation of sea stars, jellies, and corals among all public websites of institutions accredited by the Association of Zoos and Aquariums (AZA) (N = 237). Among the organizations with an aquarium (n = 125), only 55 (44 percent) profiled at least one of the three animals, resulting in 89 total profiles. Five general approaches to characterizing these animals emerged: (1) scientific social distancing, (2) beautiful and eye-catching, (3) grotesque, otherworldly, and strange, (4) brainless beauties, and (5) objects of touch, entertainment, and experience. While some practices, like touch exhibits, can support empathy outcomes among visitors, online profile practices may contribute to the objectification of these animals among visitors, which could ultimately impact conservation attitudes, intentions, and behaviors. Full article

Atopic dermatitis (AD) is a common chronic skin disease affecting both children and adults. Currently lacking a clinical cure, AD presents significant physical and emotional challenges for patients and their families, substantially impacting their quality of life. This underscores significant unmet needs in AD management and highlights the necessity for developing effective therapeutic applications. Recently, several chlorine-containing active substances with promising pharmacological activity have been discovered in soft corals cultivated through coral farming. Among these, brianolide, isolated from the soft coral Briareum stechei, has shown promising potential. This study investigated brianolide’s regulatory effects on the inflammatory response in atopic dermatitis and its underlying mechanisms. Using an in vitro human keratinocyte cell line (HaCaT) stimulated with tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ) to mimic AD inflammation, brianolide was found to inhibit cytokine and chemokine expression via the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NFκB)-signaling pathways. In an in vivo animal model of 2,4-Dinitrochlorobenzene (DNCB)-induced AD, brianolide demonstrated anti-inflammatory effects, reducing transepidermal water loss (TEWL), ear thickness, erythema, and epidermal blood flow. These findings provide new insights into brianolide’s activity against AD-related inflammation, elucidate potential mechanisms, and contribute to understanding the pharmacological potential of natural coral products for AD treatment. Full article

Zoos and aquariums house a wide range of species, yet research remains heavily skewed toward mammals and camera-based technologies. This systematic literature review examines the use of computing technologies to monitor or interact with animals in zoos, aquariums, or wildlife parks, with a focus on taxonomic representation and technological diversity. A total of 125 studies published between 2014 and 2024 met the inclusion criteria, encompassing 151 recorded instances of technology use. Cameras and video systems were the most frequently used tools, appearing alone in 40% of studies and in combination with other technologies in an additional 18.4%, accounting for 48.3% of all technology instances. Most studies focused on only mammals (73.5%), and behavioral monitoring was the most common research aim (40.9%). These findings suggest an uneven distribution of research shaped more by convenience and familiarity than by welfare need, highlighting a critical opportunity to diversify both species focus and technological application. Increased investment in underrepresented species and underutilized tools will help ensure that research better reflects the full spectrum of animal needs and experiences. Full article

The welfare of animals in zoos has come under increasing scrutiny as public awareness grows around the biological needs of captive species. It is also becoming clear that promoting positive welfare experiences upholds population management and conservation aims. This paper re-evaluates current welfare frameworks in zoological institutions, advocating for evidence-based practices, multi-dimensional welfare metrics, and greater emphasis on species-specific needs, as well as the importance of input-based approaches to assess the welfare of zoo animals. By evaluating the limitations of current welfare practices (e.g., a lack of species-specific assessment protocols or sound husbandry evidence to base measures on) and presenting potential areas for improvement, this paper identifies ways that sound baselines for meaningful zoo animal welfare outputs can be created. Although current welfare policies from large zoo membership organisations stipulate assessment of welfare outputs as key to improving animal welfare standards, such outputs can only be positive if inputs are species-specific and relevant to the animals being housed. Practices such as the use of environmental enrichment (for example) need to be further refined to ensure they provide meaningful outputs (for the individuals) from the inputs that create them. Understanding the animal’s needs to ensure that the goal of enrichment is clear benefits both the animal who is provided with the enrichment and the human caregivers as husbandry and management becomes easier. A focus on welfare outputs is commendable and (especially when considering emotional outputs) is indeed a gold standard to aim for, yet we must not lose sight of striving for improvements to housing, husbandry, and species-specific care. Without such fundamental support from correct inputs, outputs are unlikely to be truly (or meaningfully) positive. Therefore, consistent re-examination of inputs is required to make sure they uphold an individual’s attainment of good welfare. Full article

Zoo, aquarium, and sanctuary exhibit designers, both specialist “zoo architects”, and general practice architects, as well as landscape architects generally do not closely follow the evolving scientific literature on zoo biology, visitor experience, and managed animal welfare. Reportedly, this is because most zoo and aquarium clients do not require these efforts. Detailed requirements are provided by clients as project programs or briefs, which vary widely in quality and currency. Many clients and designers copy or adapt popular enclosure models without regard to their scientific foundations. Research papers frequently focus on discrete subject areas, such as animal behavior and welfare, visitor experience, or education, using their own methods and vocabulary. Relatively few studies integrate findings in ways useful to designers in preparing widely integrated systems. Regulatory standards set minimum rather than ideal standards. Knowledge of in situ animal behavior is lacking for many managed species. How can zoo and aquarium managers and designers be encouraged to increase research within the design process? This review article suggests that the long-term benefits of greater and better science integration outweigh initially higher design costs, resulting in improved facility and management design, benefiting all zoo, aquarium, and sanctuary stakeholders, and providing factual evidence underpinning community support. Full article

Augmented Reality (AR) seamlessly blends the real-world environment with digitally generated content, creating an interactive hybrid experience where both realities coexist. This paper explores an augmented reality application developed for natural heritage education, specifically designed to enhance indoor learning. The focus is on a learning activity titled Exploring the Aquarium, implemented by the Education Centre for the Environment and Sustainability (E.S.E.C.) of Kastoria as part of an environmental education program. The activity enriches students’ knowledge and experiences during their aquarium visit, fosters active participation in the learning process, stimulates cognitive interest, and encourages actions that support the ecological restoration of aquatic ecosystems. This paper presents the application’s design criteria, thematic focus, learning objectives, and core functionalities. Additionally, the paper presents findings from quantitative research evaluating the learning experience. A questionnaire tailored for AR applications was employed to assess aspects such as challenge, educational value (knowledge gained), user collaboration, and intention to reuse the app. Data were collected from 148 K-12 students during the 2023–2024 school year. Descriptive statistical analysis revealed that all factors were evaluated highly. The results indicate that the AR-enhanced educational activity captured the students’ interest and facilitated a collaborative learning environment. The application was positively rated for its functionality, usability, informational content, and the satisfaction it provided, as well as its ability to encourage cooperation and future reuse. Full article