Search Results (7,549)

XYLEM CYSTEINE PROTEASE 1 (XCP1) is a cysteine protease that plays a critical role in xylem differentiation and tracheary element (TE) formation. Our previous study demonstrated that TE remodeling occurs at the graft union in the Arabidopsis thaliana (At)/Nicotiana benthamiana (Nb) interfamilial graft. Here, we identify that the AtXCP1 transcript is specifically localized in TEs at the graft interface of the incompatible At/Nb interfamilial grafts, and its expression is reduced in these incompatible grafts compared to the compatible grafts. Analysis of AtXCP1pro::GFP reporter lines revealed that AtXCP1 expression is rapidly induced by wounding at the graft interface in At/Nb interfamilial grafts during the early grafting stage. Notably, AtXCP1 expression was significantly stronger in At/Nb heterografts than in At/At homografts, and GFP fluorescence was observed in the stock xylem at 7 days after grafting (DAG) in heterografts, a dynamic process absent in At/At homografts. We found that the Atxcp1 mutant promoted the survival of At/Nb interfamilial grafts during the early grafting stage but decreased the survival after several months, indicating delayed incompatibility. Anatomical examination revealed that large cellular deposits accumulated at the graft interface in Atxcp1/Nb interfamilial grafts and exhibited abnormal TE morphology at later stages. Our findings identify AtXCP1 as a key regulator of xylem reconnection and delayed incompatibility in At/Nb interfamilial grafts. Full article

Interleukin-33 (IL-33) is an IL-1 family cytokine that functions as an alarmin and contributes to inflammatory responses, immune regulation, and tumor-associated processes through the IL-33/ST2 signaling axis. In this study, IL-33-specific nanobodies were isolated from a synthetic phage display library and further engineered into bivalent tandem formats to improve their binding performance. Five representative monovalent nanobodies showed concentration-dependent binding to IL-33, with SPR-derived K_D values ranging from 3.6 × 10⁻⁸ to 2.81 × 10⁻⁷ M. Among the engineered bivalent constructs, Nb1–Nb2 exhibited the strongest apparent binding affinity, mainly due to a markedly reduced dissociation rate. Competitive SPR analysis indicated that Nb1 and Nb2 show largely compatible binding to IL-33, consistent with distinct or minimally overlapping binding regions, supporting their selection as a hetero-bivalent pair. In a preliminary wound-healing assay using HT-29 colorectal cancer cells, Nb1–Nb2 attenuated IL-33-induced wound closure under low-serum conditions. These results indicate that hetero-bivalent engineering can enhance the apparent binding affinity of IL-33-targeting nanobodies and provide a useful molecular tool for further investigation of IL-33-associated biological responses. Full article

Severe Combined Immunodeficiency (SCID), Spinal Muscular Atrophy (SMA), and X-Linked Agammaglobulinemia (XLA) are rare but life-threatening genetic disorders in infants that can lead to severe infections, progressive neuromuscular degeneration, or severe immune dysfunction associated with significant morbidity and mortality if not diagnosed early. Advances in newborn screening (NBS) technologies have enabled pre-symptomatic detection of these conditions, allowing early initiation of life-saving interventions such as hematopoietic stem cell transplantation, gene therapy, and immunoglobulin replacement therapy. However, the absence of a standardized national clinical pathway linking screening, confirmatory testing, and specialist referral in Malaysia continues to contribute to delayed diagnosis and suboptimal patient outcomes. This review examines and synthesizes current evidence on the clinical pathways for early diagnosis and management of SCID, SMA, and XLA, with particular emphasis on diagnostic workflows, screening technologies, and healthcare system challenges within the Malaysian context. The review examines disease epidemiology, consequences of delayed diagnosis, and the role of expanded NBS under the Screening for Health, Intervention, Nurturing of Every Child (SHINE) program in improving early diagnosis and management. In addition, the paper outlines the current NBS landscape, the use of multiplex real-time polymerase chain reaction (PCR) assays for simultaneous detection of T-cell receptor excision circles (TREC), kappa-deleting recombination excision circles (KREC), and survival motor neuron 1 (SMN1) gene deletion of exon 7 from dried blood spot (DBS) samples. A structured diagnostic framework incorporating screening interpretation, confirmatory testing, and urgency-based referral pathways is also proposed. By addressing current operational barriers and coordinating laboratory referral systems, expanding NBS programs could significantly improve early diagnosis and long-term outcomes for infants affected by SCID, SMA, and XLA in Malaysia. Full article

Decommissioned airfields are increasingly recognized as strategic sites for ecological regeneration, climate adaptation, and the creation of new public spaces. However, research on their transformation has predominantly focused on the environmental performance of Nature-based Solutions (NBS), often overlooking the role of inherited spatial morphology in structuring regeneration processes and outcomes. This paper proposes an AI-assisted, morphology-based proto-ontological framework for analyzing and designing post-airfield architecture. The framework was developed through the inductive and comparative analysis of a corpus of 32 urban post-airfield regeneration projects, from which recurrent inherited morphologies, transformation actions, spatial devices, and NBS were identified and structured into a relational sequence. The framework was then applied to two contrasting case studies: Maurice Rose Airfield Park (Frankfurt) and Xuhui Runway Park (Shanghai); these were selected for their different transformation logics. The results show that similar airfield morphologies can generate markedly different climatic, ecological, social, and memory-related outcomes depending on how they are transformed and linked to NBS. The study demonstrates that inherited airfield morphologies are not passive remnants but operative spatial structures, and that NBS should be understood as spatially embedded and form-generating design components. The proposed proto-ontology offers a transferable analytical model and a basis for future computational and generative design applications. Full article

Quaternary β-Ti-xTa-xZr-xNb (TTZN) alloys (x = 10, 20, and 30 wt%) were surface-modified by plasma electrolytic oxidation (PEO) to improve their surface properties. This treatment promotes the incorporation of bioactive ions, such as Ca and P, and favors the formation of a porous anodic surface resulting from the oxidation of the precursor metals. This study investigated how the addition of alloying elements (Zr, Ta, and Nb) influences oxide formation, PEO-induced pore morphology, wettability, and coating hardness. The surfaces were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS), Vickers microhardness testing, and wettability analysis. XRD analysis revealed that the TTZN10 alloy exhibited crystalline TiO₂ phases in the form of anatase and rutile. In contrast, the TTZN20 and TTZN30 alloys exhibited only cubic ZrO₂ diffraction peaks, while no TiO₂ peaks were detected within the detection limits of the XRD technique. Micrographs showed micrometric pores on all alloy surfaces. The TTZN20 alloy exhibited the highest porosity (31.8%), which correlated with lower hydrophilicity (θ = 79°) and high surface free energy (67 mJ/m²). After PEO treatment, all surfaces exhibited high hardness values ranging from 491 to 561 HV. The highest hardness was observed for TTZN10, attributed to the mixed anatase/rutile TiO₂ phase composition. Full article

Rice (Oryza sativa L.) production is constantly threatened by devastating diseases such as rice blast, bacterial blight, and brown planthopper infestation. The AP2-type transcription factor OsHTR (also known as SMOS1/SHB/RAL1/NGR5/GR5) has been previously implicated in hormonal signaling networks and nitrogen use efficiency; however, its role in disease resistance remains largely unexplored. In this study, we functionally characterized OsHTR in disease resistance using knockout (KO) and overexpression (OE) transgenic lines in the ZH11 background. Transcriptome analysis revealed that differentially expressed genes in the htr mutant were significantly enriched in plant–pathogen interaction pathways, with multiple NBS-LRR and NB-ARC resistance-related genes upregulated. Real-time PCR validation confirmed the upregulation of 15 candidate resistance genes in the htr mutant. Comprehensive resistance evaluations suggested that HTR-KO lines exhibited enhanced resistance to rice blast and bacterial blight compared to wild-type ZH11 and HTR-OE lines, which displayed moderate susceptibility. In contrast, all lines remained highly susceptible to brown planthopper, indicating a disease-specific regulatory function of OsHTR. Furthermore, targeted knockout of individual upregulated resistance-related genes (LOC_Os10g04090, LOC_Os12g29690, LOC_Os02g11980, and LOC_Os11g11770) and OsHTR-interacting gene LOC_Os06g03710 confirmed their distinct contributions to blast and bacterial blight resistance but did not establish them as direct targets of OsHTR. Collectively, our results indicate that OsHTR functions as a negative regulator of disease resistance in rice, likely acting through transcriptional repression of defense-related genes, although direct binding remains to be demonstrated. This study uncovers a novel regulatory module connecting AP2-type transcription factors to disease resistance and provides valuable genetic resources for molecular breeding of broad-spectrum-resistant rice cultivars. Full article

Background: Viral attachment mediated by host cell surface receptors is the first step in viral infection. As a key cell surface receptor, heparan sulfate (HS) mediates the attachment and entry of numerous non-enveloped viruses in livestock, thereby serving as a crucial molecular target for studying virus–host interactions. Methods: Based on the structural scaffold of a nanobody (Nb; PDB: 7TJC), we rationally designed and constructed a mutant Nb targeting HS, designated HS-Mut-Nb1, using molecular docking, site-directed mutagenesis, molecular dynamics (MD) simulations, and experimental characterization. Results: Molecular docking indicated that the active site of wild-type Nb for HS binding was located within the cavity jointly formed by the complementarity-determining region 3 (CDR3) and the framework regions (FRs) of the wild-type Nb. A comprehensive analysis integrating virtual alanine scanning, site-directed mutagenesis, and MD simulations revealed that the combination of three point mutations (Phe47Arg, Asp99Tyr, and Tyr108Pro) significantly enhanced the binding affinity of Mut-Nb1 for HS, with a calculated binding free energy (ΔG) of −83.26 ± 3.06 kcal/mol. Enzyme-linked immunosorbent assay (ELISA) results further confirmed that Mut-Nb1 exhibited high affinity for HS (K_D = 65.87 nM) and specificity (positive/negative ratio, P/N = 3.84; cross-reactivity, CR < 6.60%). Conclusions: This study not only provides novel candidate molecules for elucidating the mechanism of HS–virus interactions and developing related inhibitors but also offers a reference for the rapid construction of mutant Nbs. Full article

High-entropy alloy (HEA) coatings exhibit enhanced chemical stability when doped with carbon, primarily due to the strong bonding between carbon and transition metals. Typical transition metals used in these coatings include Cr, Fe, Co, Ni, Cu, Ti, V, W, Nb, Ta, and Zr. Owing to their excellent chemical stability, HEA coatings are widely employed to protect component surfaces operating in highly corrosive environments. Against this backdrop, the present study investigates the effect of carbon doping introduced via methane gas flow during the physical vapor deposition of TiAlTaZrNb HEA coatings on corrosion resistance. The morphology and structure of the coatings were analyzed by field emission scanning electron microscopy, X-ray diffraction, and Raman spectroscopy. Corrosion protection and coating resistance were assessed through potentiodynamic polarization and electrochemical impedance spectroscopy. While increasing the methane flow resulted in an approximately 34% reduction in coating thickness, the overall coating resistance increased by one order of magnitude, reaching a maximum at a methane flow rate of 9 sccm, corresponding to the carbon solubility limit. This improvement was evidenced by a decrease in the corrosion rate from 8.02 × 10⁻² mm y⁻¹ for the uncoated H13 steel to 8.00 × 10⁻⁴ mm y⁻¹ for the HEA-coated samples. However, at higher methane flow rates, carbon precipitation and the formation of parallel microcracks contributed to an increase in corrosion rate. Full article

Herein, p-NbSe₂ thin films were deposited onto n-Si substrates to fabricate an n-Si/p-NbSe₂ (SNS) heterojunction for visible light communication (VLC) applications. Structural analysis revealed that the NbSe₂ films possess a trigonal phase and are composed of slightly elongated and irregularly shaped grains with an average size of 0.131 μm. Electrical characterization showed that the SNS heterojunction exhibits pronounced rectifying behavior, with a bias-dependent asymmetry factor reaching 6.6 × ${10}^3$. The photodetection performance of the device was evaluated under illumination from white, blue, red, tungsten, and infrared LEDs. The device exhibited excellent photodetection characteristics across the visible region, achieving a maximum responsivity of 3.79/3.68 AW⁻¹, external quantum efficiency of 1160/809%, noise equivalent power of 4.43 × 10⁻¹⁴ /4.57 × 10⁻¹⁴ WHz^−1/2, and specific detectivity of 3.91 × 10¹²/3.79 × 10¹² Jones under blue/white light illumination, confirming its practical relevance for VLC systems. In addition, frequency-dependent photocurrent measurements under modulated blue and white LED illumination revealed −3 dB bandwidths of approximately 775 Hz and 716 Hz, respectively, supporting the potential of the n-Si/p-NbSe₂ photodiode for low-frequency VLC-related visible-light detection. Compared with previously reported photodiodes used in VLC and IR technologies, the present device demonstrated superior responsivity and EQE%, together with competitive NEP and detectivity. The enhanced performance is attributed to efficient photocarrier generation and collection across the Si/NbSe2 heterojunction. These results confirm that the fabricated SNS photodiode is a promising candidate for high-sensitivity and efficient visible light communication applications. Full article

Sea-level rise is accelerating coastal erosion and storm-driven flooding, increasing risks to estuarine ecosystems and coastal communities. Nature-based solutions (NbS), such as those including ecosystem restoration, are widely endorsed for climate change risk mitigation, yet their protective performance under rising sea levels remains poorly quantified across future scenarios. Here we combined scenario-based modelling with spatially explicit exposure mapping to assess how saltmarshes influence shoreline vulnerability under three Intergovernmental Panel on Climate Change (IPCC) Shared Socioeconomic Pathways (SSP) sea-level rise projections for 2050 and 2100. Using the InVEST Coastal Vulnerability Model and the Lima estuary (NW Portugal) as a case study, we showed that existing saltmarshes currently reduce mean shoreline exposure by approximately 5%, but this contribution declines with sea-level rise, falling to 2.6% by 2100 under SSP5-8.5, resulting in an increase in areas subject to High and Very High exposure risk. But under a saltmarsh revegetation scenario, model results indicated that this revegetation significantly increases the protection across all future scenarios, reducing the number of shoreline points in High and Very High exposure classes by up to 58% and lowering the potential coastal population exposure by up to 27% by 2100 under SSP5-8.5. However, the protective effect of saltmarshes diminished under the most extreme sea-level rise trajectories, indicating that saltmarsh revegetation alone may not be enough to fully offset accelerating coastal hazards. Our results demonstrate that saltmarsh restoration can deliver meaningful climate adaptation benefits; however, to safeguard estuarine systems and coastal communities under accelerating climate change in the long term, restoration actions must be integrated into broader adaptation strategies. Full article

As a rare metal element, niobium is widely used in steel, electronics, aerospace and many other fields. Eschynite is one of the most important niobium-bearing minerals in Bayan Obo niobium ores. Investigating the beneficiation process and associated reagents is of great significance for improving niobium resource utilization. In this study, mixed ore with eschynite as the main niobium-bearing mineral was used as the research object. Under the condition that the Nb₂O₅ grade of the feed ore was 0.37%, a niobium concentrate with an Nb₂O₅ grade of 5.250% was obtained through one rougher stage and four cleaner stages, followed by magnetic separation. The content of eschynite in the niobium concentrate increased from 0.76% in the run-of-mine ore to 26.32%, with an enrichment ratio of 34.63 times, and the proportion of eschynite in all niobium-bearing minerals rose from 50.67% to 86.10%. Experimental results show that the combined flotation–magnetic separation process can realize the efficient concentration of niobium minerals dominated by eschynite, providing a technical reference for the subsequent development and utilization of eschynite-type niobium ore resources. Full article

The digital transformation of tourist facilities requires careful selection of technologies that can provide secure, stable and scalable network infrastructure. Due to the possibility of application in different sectors with different specificities, the focus of the research was placed on the implementation of smart tourist services. A hybrid multi-criteria decision-making model based on PIPRECIA and MVA models was applied for the research. Based on the literature and the opinions of experts in the field, evaluation criteria such as bandwidth, latency, energy efficiency, security and privacy, scalability, costs and interoperability were defined, and internet technologies such as Li-Fi, Wi-Fi 7, Wi-Fi 6, private 5G networks, Ethernet-over-Power (EoP), NB-IoT and LoRaWAN were defined. The results obtained put the security and privacy criterion at the top (0.2253), followed by scalability (0.1952) and bandwidth (0.1624). The obtained results indicate that Wi-Fi 7 achieved the highest weighted score (4.2247), followed closely by Li-Fi (4.2177) and Wi-Fi 6 (4.0771). Wi-Fi 7 demonstrated particularly strong performance in scalability, interoperability and bandwidth, making it highly suitable for environments with high user density. Li-Fi achieved very high scores in security and latency, which makes it particularly appropriate for security-sensitive smart tourism environments. Lower-ranked technologies such as NB-IoT and LoRaWAN proved valuable for supporting IoT and monitoring functions, rather than as primary communication infrastructure. The proposed model has proven to be a flexible, transparent and practical tool for strategic decision-making in the field of smart tourism. In addition to the basic application presented in the paper, the model has the potential to be adapted to different contexts and expanded with additional criteria or new technologies. The proposed hybrid approach can serve as a useful decision-making tool for tourism managers, system engineers and urban planners who are looking for optimal solutions for the development of digital infrastructure. Full article

In this study, manual welding electrodes with varying niobium (Nb) contents (0, 0.05, and 0.1 wt%) were developed for 960 MPa grade low-alloy high-strength steel, and deposited metals were produced through multilayer multipass welding. Microstructural characterization and mechanical testing were performed using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), electron backscatter diffraction (EBSD), and a universal testing machine to investigate the influence of Nb content and elucidate the strengthening mechanisms. The results demonstrate that under identical welding conditions, multipass thermal cycles induced a primary microstructural transformation from martensite to tempered martensite in all deposited metals, which predominantly comprised tempered martensite with minor fractions of bainite and second-phase particles. Increasing Nb content led to significant grain refinement. The second-phase particles exhibited sizes of 0.158 μm, 0.176 μm, and 0.168 μm, respectively, with volume fractions of 5.69%, 5.82%, and 5.90%. Nb addition substantially enhanced hardness and strength while causing a noticeable reduction in low-temperature impact toughness, though the values remained within acceptable limits. The deposited metal containing 0.05 wt% Nb exhibited optimal comprehensive mechanical properties, with a hardness of 386.7 HV, tensile strength of 1060 MPa, yield strength of 962 MPa, and Charpy impact energies of 41.95 J and 33.17 J at −40 °C and −60 °C, respectively. Theoretical calculations revealed that the dislocation strengthening contribution in martensite increased from 526 MPa to 600 MPa with increasing Nb content, representing the dominant strengthening mechanism, while grain refinement strengthening increased from 135.5 MPa to 157.6 MPa. Full article

The present work reports on the microstructural and micro-mechanical characterization of Ti-Nb-HA-based composites. The composites were prepared via a spark plasma sintering (SPS) consolidation process. The effect of two distinct levels of hydroxyapatite (HA) content (e.g., 10 and 20 wt.%) on the microstructural and micro-mechanical properties were investigated via in situ micro-pillar compression, and the results were compared against a sole Ti-Nb composite. The microstructure of the composites was composed of parent Ti and Nb grains, together with the reaction products; due to the decomposition of HA, there was a rise in different biocompatible phases. The Vickers hardness of the composite was sensitive to applied loads due to the presence of pores and voids, which was foreseen to be beneficial when the composite was used as an implant, according to the literature. The addition of 20 wt.% HA causes a decrease in hardness to 990 HV, compared to 1109 HV for 10 wt.% HA and 1275 HV for sole Ti-Nb. The addition of HA into Ti-Nb also lowers the compressive strength from 553 MPa for Ti-Nb to 189 MPa for Ti-30Nb-20HA. This was accompanied by a reduction in the elastic modulus, from 130 GPa for Ti-Nb to 29 GPa for Ti-30Nb-20HA. The deformation mechanism was ductile-dominated in all cases, with the presence of a quasi-brittle nature for HA-containing composites. Full article

In this study, the Nb from the lightweight Ti₆₅(AlCrNbV)₃₅ medium-entropy alloy was replaced with Zr to create lower-density Ti_x(AlCrZrV)_100−x (x = 65, 67, 70, or 75) alloys. All alloy ingots were fabricated through vacuum arc melting and drop casting. X-ray diffraction analysis revealed all as-cast alloys exhibited only a single body-centered cubic structure. As the Ti content increased, the strength of the as-cast alloys decreased from 1247 to 981 MPa, whereas their elongation marginally improved. Moreover, the mechanical properties of these alloys were considerably enhanced through thermomechanical treatment (50% hot rolling and 80% cold rolling) and then rapid annealing at 700 °C, 800 °C, or 900 °C. An increase in the annealing temperature led to a notable decrease in the yield strength of the alloys but a considerable increase in their ductility. Ti65, Ti67, and Ti70 alloys annealed at 700 °C or 800 °C exhibited a yield strength of ≥1200 MPa and a ductility of ≥10%. Of the fabricated alloys, the Ti67 alloy annealed at 700 °C exhibited the optimal mechanical properties (yield strength of 1552 MPa and ductility of 13.6%). It exhibited low density (4.89 g/cm³) and a specific yield strength of 317 MPa·cm³/g, thus demonstrating considerable potential for transportation and energy applications. Full article