Search Results (7,589)

Replicating the authentic masticatory properties of conventional animal meat remains a primary technical bottleneck for sustainable plant-based analogues. To address critical textural deficiencies like structural fragmentation, this study systematically optimized plant-based patty formulations. The independent and interactive effects of textured fibrous soy protein (TFSP), water, and konjac glucomannan (KGM) were quantified using single-factor experiments and Response Surface Methodology (RSM). Single-factor experiments revealed that springiness peaked at 60 g TFSP, 15 g water, and 10 g KGM, respectively, with excessive additions of each component resulting in structural network disruption. Designating springiness as the core metric, a reliable quadratic regression model identified the optimal matrix: 63.36 g TFSP, 14.39 g water, and 8.57 g KGM. Empirical validation achieved a maximum springiness of 1.56 mm and hardness of 5.51 N, with a negligible relative error (1.27%) from theoretical predictions. Mechanistically, KGM functioned as an active polymeric filler, interacting synergistically with hydrated protein fibers via hydrogen bonding and hydrophobic associations to reinforce the structural network. Comparative Texture Profile Analysis demonstrated that the optimized PBP exhibited a tender masticatory profile with hardness and springiness approximating conventional beef patties, while presenting lower chewiness and higher adhesiveness attributable to the water-binding capacity of KGM. Ultimately, this research provides mathematically validated engineering parameters and theoretical insights into protein–polysaccharide phase behaviors to facilitate the industrial manufacturing of premium plant-based meats. Full article

Somatostatin is a potent endocrine regulator and neurotransmitter, exerting predominantly inhibitory effects in different tissues of the body, via G-protein coupled receptors. Five such specific receptors have been identified, with different effects and tissue distribution. The multifaceted actions and effects of somatostatin make it useful as a potential therapeutical means in various pathologies; however, in clinical practice, somatostatin analogues, namely octreotide, lanreotide and pasireotide, are commonly used instead, due to their increased half-life and increased receptor selectivity, with pasireotide showing a more extensive receptor binding profile and high affinity for somatotastin receptor (SSTR) 5, which may prove effective in cases of resistance to first-generation analogues. Apart from their many uses in neoplastic pathologies, somatostatin analogues represent viable treatment choices in some ocular pathologies, congenital hyperinsulinism, gastrointestinal bleedings and portal hypertension, acute pancreatitis, and dumping syndrome. They have also been used in some cases, with varying degrees of success, in patients with post-surgical gastrointestinal and lymphatic fistulas, refractory chronic diarrhoea and polycystic kidney disease; many applications in paediatric patients have also been documented. The aim of this review is to present the applications of somatostatin and its analogues as alternative or second-line therapies, along with insights into their effectiveness and future potential. Full article

Modeling milk yield in dairy cows is essential for improving management decisions, but traditional lactation curve models often fail to capture individual variability. Machine learning approaches offer greater flexibility; however, their performance in small, within-herd datasets and their reliance on explicit cow identification remain unclear, particularly in grazing systems. This study aimed to evaluate whether routinely measured biological traits can substitute for cow identification in machine learning models for predicting daily milk yield within a herd under limited data conditions. The dataset comprised 62 lactations from 48 Holstein–Friesian cows in a grazing system. Two machine learning models were developed: one including cow identification (With ID) and another excluding cow identification but incorporating milk quality traits, body weight, and body condition score (Without ID). Both models were compared with the Wood lactation model fitted to individual cows. The With ID and Without ID models achieved $R^2$ values of 0.97 and 0.93 and RMSE values of 1.2 and 1.6 ${\mathrm{kg} \mathrm{d}}^{-1}$, respectively. Both machine learning models outperformed the Wood model fitted individually to each cow ($R^2$ < 0.90; RMSE > 2.03 ${\mathrm{kg} \mathrm{d}}^{-1}$), which represents an implicitly cow-specific approach. The model including cow identification therefore served as a machine learning analogue to this benchmark. Importantly, the trait-based model closely matched the performance of the cow-specific model. These results demonstrate that machine learning models based on routinely measured traits provide a practical approach for predicting within-herd milk yield from small datasets, while retaining much of the accuracy of cow-specific models. Full article

Two metallacyclic tetranuclear Fe(III) complexes, [{Fe₂(μ-O)(μ-RCOO)₂(tpon)}₂](BPh₄)₄ [R = Me (1), Ph (2)], where the flexible ditopic ligand tpon (N,N,N′,N′-tetrakis(2-pyridylmethyl)octane-1,8-diamine) links two μ-oxo-bis(μ-carboxylato) triple-bridged dinuclear units, have been prepared. Single-crystal X-ray diffraction establishes that both complexes adopt a 26-membered macrocyclic framework featuring an internal cavity capable of guest inclusion. Notably, incorporation of a monoatomic μ-oxo bridge enforces an outward orientation of the ligand alkyl chains, thereby suppressing the “zipper effect” observed in the previously reported Mn(II) analogue and facilitating the encapsulation of an acetone molecule. UV–vis absorption and diffuse-reflectance spectra confirm that the tetranuclear scaffold remains intact in both the solid state and in solution. These results demonstrate that modulating local coordination directionality via μ-oxo bridging is an effective strategy for controlling the global conformation and host–guest properties of large metallasupramolecular architectures. Full article

Background: Post-stroke upper-limb spasticity can cause pain, hinder passive care, and lead to secondary musculoskeletal complications. Current minimally invasive treatments have important limitations. Cryoneurolysis, which creates a controlled cold lesion of peripheral nerves, may offer a partially reversible focal denervation alternative. Methods: We conducted a feasibility case series in the outpatient department of a rehabilitation centre. Three adults with chronic post-stroke hemiparesis and a non-functional spastic upper limb underwent ultrasound- and nerve stimulation-guided cryoneurolysis of the musculocutaneous, median, and/or ulnar nerves. All had demonstrated a positive response to diagnostic nerve blocks beforehand. Feasibility outcomes included completion of planned nerve targets, tolerability under local anesthesia, absence of serious adverse events, and completion of 6-month follow-up. Secondary outcomes were Modified Ashworth Scale (MAS), qualitatively assessed passive joint mobility (video-documented), pain measured by visual analogue scale, sensory testing, and electroneuromyography (ENMG). Results: All procedures were completed as planned. Treatment was well tolerated under local anesthesia, and no serious adverse events occurred. MAS decreased by at least 2 points in targeted patterns, with immediate improvement in passive mobility; these effects persisted at 6 months. Pain remained unchanged in two participants and improved in one. Sensory testing at 6 weeks was stable. ENMG findings were heterogeneous, including reduced ulnar sensory action potential amplitude and biceps denervation activity in one participant. Conclusions: In this small series, cryoneurolysis for post-stroke upper-limb spasticity was feasible and associated with sustained tone reduction and improved passive mobility. Larger controlled studies are required to better define safety, optimize targeting strategies, and assess patient-centred outcomes. Full article

Background/Objectives: Since 2016, the mutation of isocitrate dehydrogenase 1 (mIDH1) enzymes has become a major molecular marker for glioma classification and diagnosis. Moreover, the recent success of the INDIGO clinical trial on AG-881 (vorasidenib^®), an aminotriazine-based mutated IDH1/2 inhibitor (IC₅₀ = 6 nM/12 nM), validated the need for noninvasive detection of mIDH1 in brain tumors. This work is based on developing a series of novel fluorinated analogues of AG-881 and evaluating their potential in mIDH1 PET detection. Methods: The analogues were tested for their potency and then the best candidate was radiofluorinated and used for in vitro cell uptake studies. Results: Six analogues (6–11) were designed and synthesized, but only compound 6 showed nanomolar inhibitory potency towards mIDH1 (IC₅₀ = 400 nM). Following successful radiofluorination, in vitro cell uptake studies showed no selective accumulation of [¹⁸F]6. Conclusions: This study highlights the critical impact of substituent positioning and halogen substitution within the pyridyl moiety on maintaining inhibitory potency. Further medicinal chemistry research is needed to develop an aminotriazine-based ¹⁸F-radiolabeled mIDH1 ligand. Full article

Background: Scabies is a highly contagious ectoparasitic skin infection that is considered a global public health problem. Treatment primarily focuses on mite eradication, but an inflammatory phase may persist despite successful therapy, known as post-scabietic pruritus (PSP). This study aimed to investigate the clinical features, duration, and severity of PSP in patients with persistent symptoms despite successful treatment. Methods: This cross-sectional descriptive study included 43 adult patients with dermoscopically documented scabies resolution, who reported pruritus persisting for more than 28 days. Demographic and clinical data, including clinical characteristics, treatment history, and pruritus severity assessed by the Visual Analogue Scale (VAS), were collected via structured questionnaires. Results: PSP persisted for 30–365 days, and 48.8% of patients experienced pruritus longer than 45 days. Pruritus severity in 72.1% of patients was reported as a VAS score ≥8. Nodular lesions were observed in 55.8% of patients. Sixteen patients (37.2%) had an atopic background, and nine patients (20.9%) reported a house dust mite (HDM) allergy. Heat, hot baths, and sweating were the most frequent aggravating factors. Despite persistent symptoms, 44.2% of patients reported not receiving any specific treatment after mite eradication. Conclusions: PSP can be prolonged and severe. Nodular lesions may be more frequent than previously reported. Eradication of the mite is the first step, and treatment is not complete until the post-treatment inflammatory phase is controlled. Full article

Background: Skin carcinoma is among the most common cancers globally, necessitating the urgent development of innovative chemotherapeutic drugs that exhibit great selectivity and diminished toxicity towards normal cells. This study assessed a series of recently synthesized compounds (4–15) for screening their anticancer efficacy against A 431 human skin carcinoma cells to find effective and selective treatment candidates. Methods: Compounds were synthesized via a one-pot, three-component reaction. Cytotoxicity was evaluated using the MTT assay, with 5-fluorouracil (5-FU) as the reference standard, which exhibited potent activity against A 431 skin cancer cells. The activity of these drugs against normal BJ cells was evaluated, with mechanistic investigations encompassing topoisomerase I/II enzyme inhibition, cell-cycle analysis, and Annexin V–FITC/PI apoptosis assays. Results: Most compounds exhibited dose-dependent cytotoxicity, with compound 14 demonstrating the highest potency (IC₅₀ = 76.7 µg/mL), exceeding that of 5-FU (IC₅₀ = 83.7 µg/mL) while preserving selectivity for BJ cells. Compound 14 exhibited moderate inhibition of topoisomerases I and II (IC₅₀ values of 17.5 and 17.3 µM), as confirmed by docking studies. Flow cytometry indicated G0/G1 phase arrest (64.09% vs. 58.18% in control), while apoptosis assays confirmed induction of both early and late apoptosis, accompanied by significant necrosis. Conclusions: Compound 14 is the most efficacious and selective drug in this series, functioning through topoisomerase inhibition, G0/G1 cell cycle arrest, and apoptosis, thereby representing a strong candidate for subsequent preclinical research. Full article

We report a curious numerical observation: If atomic nuclei are modelled as connect-sums of threefoil knots with alternating chirality, the ropelength of the composite knot—a purely geometric quantity requiring no quantum mechanics—tracks the experimental binding-energy curve from hydrogen to uranium. A two-parameter fit to 50 nuclei gives $R^2=0.9998$ (coefficient of determination; 1 = perfect fit) and $\mathrm{RMS}=6.9\mathrm{MeV}$ (root-mean-square deviation between model and experiment), comparable to the five-parameter Bethe–Weizsäcker formula ($\mathrm{RMS}=8.3\mathrm{MeV}$) at less than half the parameter count. Out-of-sample predictions for ${}^{244}\mathrm{Pu}$ and ${}^{252}\mathrm{Cf}$, not used in the fit, are accurate to $0.4\mathrm{MeV}$ and $8.4\mathrm{MeV}$, respectively. What makes the observation worth reporting is not the fit itself, but the range of nuclear phenomenology that emerges uninstructed from the topology: saturation, surface energy, isospin pairing, odd-even staggering, and geometric analogues of nuclear isomers all appear as consequences of the connect-sum construction, without additional assumptions. We catalogue these correspondences, assess which are structural and which may be coincidental, and identify concrete numerical tests that would distinguish the two possibilities. Full article

Background: This study aimed to examine whether a physical education program based on the ecological dynamics approach, implemented through small-sided games (SSG), produces greater improvements in motor skills, daily physical activity levels, and perceived physical fitness in middle school students. Methods: Forty-eight students were assigned to an SSG group (ecological dynamics lessons including small-sided games, n = 26) or a Control group (traditional lessons based on teacher-centered instruction and analytical exercises, n = 22). The intervention lasted 12 weeks, with two sessions per week. Motor performance was assessed using the standing broad jump, 5-standing broad jump, 20 m sprint, 10 × 5 m shuttle run, 5-0-5 agility test, and sit-and-reach test. Daily physical activity was evaluated using the International Physical Activity Questionnaire—Short Form (IPAQ-SF), and perceived physical fitness was assessed using the Visual Analogue Fitness Perception Scale for Adolescents (FPVASA). Results: Significant group-by-time interactions were found in all motor tests. IPAQ-SF data revealed significant group-by-time interactions for vigorous and moderate physical activity. Perceived physical fitness showed significant group-by-time interactions for all items except flexibility. Conclusions: Physical education lessons structured according to the ecological dynamics approach and implemented through SSG-based protocols led to greater improvements than traditional methods. The dynamic and variable nature of SSG likely enhances neuromuscular stimulation, motor engagement, and motivation during physical education lessons. Full article

The central inversion tectonic belt of the Xihu Sag is a typical inversion structural zone in the East China Sea Shelf Basin and a key target for hydrocarbon exploration. The Ningbo structure underwent five evolutionary stages—rifting, post-rift transition, depression, transpressional inversion, and regional subsidence—during which the stress regime evolved from extension to transpression-dominated strike-slip deformation. This study employs seismic interpretation, fault-throw analysis and sandbox analogue modeling to clarify its genetic mechanism and controlling factors. The results show that the fault system exhibits characteristics typical of strike-slip deformation, including high-angle master faults and well-developed flower structures. Along strike, fault throw alternates between normal and reverse displacement over short distances, forming a “dolphin effect,” reflecting spatial alternation between transtensional and transpressional domains. Comparison of three experimental models demonstrates that the overlap and lateral spacing of pre-existing basement faults primarily control deformation style. Greater overlap and closer spacing promote through-going fault linkage and the formation of a principal displacement zone, generating a narrow, continuous uplift belt. A three-dimensional genetic model is established, providing a unified explanation of structural patterns, with implications for similar inversion systems. Full article

Background: Food bank users experience food insecurity, a social determinant of health linked to poorer physical and mental health. However, evidence on the health-related quality of life (HRQoL) of food bank users in Spain is scarce. Objectives: This study sought to assess HRQoL among users of the Navarra Food Bank and identify associated sociodemographic factors. Methods: We performed a cross-sectional study of heads of household using the Navarra Food Bank. A simple random sample of 350 participants was selected from a population of 2749 families. HRQoL was assessed by telephone using the EQ-5D-5L. We described the prevalence of problems in the five EQ-5D-5L dimensions, calculated the EQ-5D-5L utility index using the Spanish value set, and analyzed EuroQol Visual Analogue Scale (EQ-VAS) scores. Associations with sociodemographic characteristics were examined using multivariable general linear models. Results: Mean EQ-VAS was 73.56 (95% CI: 71.62–75.50), and mean EQ-5D-5L utility index was 0.815 (95% CI: 0.800–0.831). The most frequently reported problems were anxiety/depression (62.9%) and pain/discomfort (55.7%), while mobility (25.5%), usual activities (19.7%), and self-care (8.7%) were less commonly affected. Older age was significantly associated with both EQ-VAS and EQ-5D-5L utility index. Employment status and nationality were significantly associated with EQ-VAS, whereas sex was significantly associated with the EQ-5D-5L utility index. Conclusions: HRQoL was impaired among users of the Navarra Food Bank, with the greatest burden observed in the anxiety/depression and pain/discomfort dimensions. Older age and selected sociodemographic characteristics were associated with poorer HRQoL. Given the cross-sectional design, the findings should be interpreted as associative rather than causal. Full article

Kappa-carrageenan (κ-CRG) forms thermo-reversible physical hydrogels via a coil–helix transition and helix bundling, but its sulfate-driven electrostatic repulsion limits mechanical robustness and control over aqueous disintegration. Here, we show that plant-derived polyphenols reprogram κ-CRG gel through sulfate-directed binding in a structure-dependent manner. Tannic acid (TA) selectively engages κ-CRG sulfate groups, yielding transparent gels and a >5-fold increase in storage modulus, whereas the same TA triggers turbidity and precipitation in sulfate-free agarose, supporting sulfate-mediated specificity. Using monomeric pyrogallol as a galloyl analogue, we demonstrate that monovalent interactions partially reinforce κ-CRG but lack cooperative stabilization. Intervention timing further separates mechanism. Pyrogallol produces pathway-dependent mechanics and gelation temperature, while TA is stage-insensitive, consistent with multivalent network annealing. In simulated gastric/intestinal fluids, pyrogallol/κ-CRG gels retain morphology longer, whereas TA/κ-CRG ones disintegrate rapidly yet exhibit strong adhesion to rough substrates and human skin. These findings provide a fully food-grade route to tune κ-CRG mechanics, thermal behavior, adhesion and programmed disintegration. Full article

Firstly, we define and study the notions of a smash product for actions of multiplier left Hopf algebras on algebras and of an integral on such smash products. Then we construct an analogue of Radford’s biproduct in the framework of multiplier left Hopf algebras under assumption of a multiplier left Hopf algebra having an anti-bialgebra homomorphic left antipode. Finally, we study a duality theorem for smash products of a left Hopf algebra of dimension n which is a special multiplier left Hopf algebra. Full article

Background: Dual mobility (DM) total hip arthroplasty (THA) was introduced to reduce postoperative instability, one of the most frequent causes of revision after hip replacement. Its use has progressively expanded beyond revision surgery to selected high-risk primary cases; however, comparative data integrating both clinical and radiographic outcomes from real-world tertiary centers remain limited. Methods: A retrospective comparative study was conducted including 78 patients who underwent THA with a DM acetabular component between January 2019 and December 2024, and 78 matched controls who received conventional fixed-bearing THA during the same period. Matching criteria were age, sex, and procedure type (primary versus revision). Clinical outcomes were assessed using the Harris Hip Score (HHS) and visual analogue scale (VAS) for pain. Radiographic evaluation focused on component positioning, radiolucent lines, and signs of loosening. Complications and revision rates were compared between groups. Results: The mean age was 71 ± 9 years, and 62% of patients were female. Mean follow-up was 38 months. HHS improved from 54 ± 10 preoperatively to 89 ± 8 postoperatively in the DM group (p < 0.001), with similar final functional outcomes in the conventional THA group (90 ± 9, p = 0.48), and comparable improvement between groups (p = 0.62). Radiographic parameters demonstrated stable fixation and appropriate component positioning in both groups, with no significant intergroup differences. The dislocation rate was numerically lower in the DM group (1.3% vs. 5.1%), although this difference did not reach statistical significance (p = 0.37). No cases of intraprosthetic dislocation occurred. Overall implant survival free from revision at five years was 96.5% for DM and 94.7% for conventional THA (p = 0.47). Conclusions: DM THA achieved excellent clinical and radiographic outcomes, with a numerically lower dislocation rate than conventional THA. Mid-term implant survivorship was comparable between groups, supporting DM as a reliable option for improving stability in appropriately selected patients. Full article