Search Results (2,793)

Surface quality of machined wood-based panels plays a key role in subsequent processing and product performance; however, its formation during CNC edge milling remains insufficiently understood, particularly for materials with different structural characteristics, including recycled content. This study investigates the influence of feed per tooth, milling strategy, and material structure on surface quality during CNC edge milling of particleboards manufactured from alternative lignocellulosic resources. Six board variants were experimentally produced and machined on a five-axis CNC machining center Morbidelli m100 using a single-edge milling cutter, with feed per tooth varied at three levels and both climb and conventional milling strategies applied. Surface quality was evaluated using a non-contact 3D optical profilometer Keyence VR-6000, and roughness (R_a) and waviness (W_z) parameters were analyzed. The results showed that surface roughness increased with increasing feed per tooth for all materials, with an increase of approximately 30%–70%. Statistical analysis confirmed a significant effect of feed per tooth and material type, while milling strategy and its interaction with material were not statistically significant. Materials with higher surface heterogeneity (CVR_a) showed increased roughness and greater sensitivity to feed. A statistically significant positive relationship was found between surface heterogeneity (CVR_a) and roughness sensitivity (ΔR_a), indicating that materials with higher surface heterogeneity (CVR_a), which likely reflects variability in their internal structure, are more sensitive to changes in feed per tooth. Full article

This study examined the life characteristics, fecundity, and innate immune response of fairy shrimp (Branchinella thailandensis Sanoamuang, Saengphan and Murugan, 2002) cultivated on Chlorella vulgaris, biofloc, dried powder Spirulina sp., and a formulated feed. These feeds were selected to compare traditional live feed (Chlorella vulgaris), microbial-based feed (biofloc), and alternative dry feed in order to identify a sustainable feeding strategy for fairy shrimp culture. The lifespans of male and female fairy shrimp reared on the biofloc diet were the longest at 44 ± 6.00 and 44.33 ± 4.67 days, respectively. Fairy shrimp reared on the biofloc diet demonstrated significantly enhanced growth, with lengths of 25.66 ± 1.75 mm in males and 27.60 ± 2.08 mm in females. The highest fecundity was also observed in the biofloc treatment, with 25 ± 6.65 broods per female, an average of 229.57 ± 2.08 eggs per brood, and a total of 5726.33 ± 1518.11 eggs per female over their lifespan. Fairy shrimp fed with Chlorella vulgaris, biofloc, and Spirulina sp. showed significantly high levels of superoxide dismutase (SOD) and lysozyme activities compared to those fed on a formulated feed, while malondialdehyde (MDA) levels, a marker of oxidative stress, were lower in these three groups. Results suggested that biofloc was a viable feed option for B. thailandensis, which positively influenced growth, lifespan, and fecundity while enhancing the innate immune function. Full article

Thraustochytrids are promising alternatives for the production of docosahexaenoic acid (DHA; C22:6 n-3), a long-chain polyunsaturated fatty acid with health benefits. For practical application of this oleaginous microorganism, an efficient cultivation method to enhance DHA production is required, which relies on several factors that support cell growth, lipid accumulation, and lipid turnover. In this study, the robust submerged fermentation of an acid- and high-temperature-tolerant strain of Aurantiochytrium limacinum was investigated. Under controlled temperature and acidic conditions (pH 4.5 and 30 °C), glucose and peptone were the best carbon and nitrogen sources for enhancing biomass and DHA production, respectively, with a glucose concentration of 60 g/L and a C/N ratio of 24 being optimal for DHA production. Applying an aeration rate of 2 vvm and an agitation speed of 300 rpm using a combination of a ring sparger and pitch-blade impeller in a stirred-tank bioreactor improved DHA production using intermittent fed-batch fermentation. The highest DHA titer was obtained at 3.01 g/L, and the DHA content in biomass was 10.69% (w/w) after intermittent feeding of cassava starch hydrolysate as the substrate. Full article

This study evaluated the effects of including spray-dried porcine plasma (SDP) in nursery diets and enzymatically hydrolyzed plasma (EHP) in drinking water on piglet growth performance and post-weaning diarrhea (PWD). Four treatments were tested: CONTROL (soy protein concentrate, SPC), P1SDP (5% SDP in phase 1), P1 + P2SDP (5% SDP and 2% SDP in phases 1 and 2), and EHP (0.88% in water during phases 1 and 2). No significant differences among treatments were observed during phase 1. During phase 2 (14–28 days) pigs fed SDP or pigs provided EHP in water had higher average daily gain (ADG; p = 0.001) and feed conversion (GFR; p = 0.013) versus the other groups. Pigs fed SDP in the first two phases had an average d-42 body weight that was 1.54 kg heavier than controls. Post-weaning diarrhea was not observed at any time during the study. These results support the use of SDP and EHP as effective nutritional strategies to enhance the growth and resilience of pigs during the post-weaning period. Both ingredients contribute to sustainable pig production by improving efficiency and promoting circular economy practices through the valorization of animal by-products. Full article

Against the backdrop of the full implementation of “antibiotic ban” and “zinc restriction” policies in livestock and poultry breeding, and the growing consumer demand for safe livestock and poultry products, the development of natural and efficient green feed additives has become crucial for the sustainable development of the animal husbandry industry. Curcumin, a natural polyphenolic compound extracted from the rhizome of Curcuma longa L., has attracted extensive attention in poultry production due to its various biological activities and safety. This paper thoroughly reviews the chemical structure and physicochemical properties of curcumin, and elaborates on its core molecular mechanisms of action, which mainly involve the regulation of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE), nuclear factor-κB (NF-κB), peroxisome proliferator-activated receptor γ (PPAR-γ), and mitogen-activated protein kinase (MAPK) pathways to exert antioxidant, anti-inflammatory, antibacterial, immunomodulatory and lipid metabolism regulatory effects. It further clarifies the practical application value of curcumin in major poultry species including broilers, laying hens, ducks and quails, showing that curcumin can significantly improve poultry production performance, optimize meat and egg quality, protect intestinal health, and enhance the ability of poultry to resist stress and diseases. Meanwhile, the review notes curcumin’s current application limitations (low bioavailability, poor stability, unclear standardized dosage, and high industrialization cost) and proposes targeted future research directions to address these issues. In conclusion, curcumin is a promising green feed additive alternative to antibiotics, and its large-scale and standardized application in poultry production will effectively promote the green, healthy and sustainable development of the poultry industry. Full article

Feed nutrition and rise in antibiotic resistance are growing global challenges in aquaculture, with Aeromonas hydrophila causing significant losses in the carp family. This 60-day study evaluated the potential of combining herbal seed extract (Achyranthes aspera and Ricinus communis) with inactivated vaccine (A. hydrophila) to enhance growth and immunity in Labeo rohita. A total of 540 fish were randomly assigned in six groups (T0-untreated control, T1-A. aspera seed extract (ASE), T2-R. communis seed extract (RSE), T3-vaccinated control, T4-vaccine plus ASE, T5-vaccine plus RSE). Results revealed that herbal–vaccine combinations, particularly T4, showed highest growth performance (p < 0.05). Furthermore, this group showed improved metabolic profiles, indicated by reduced serum cholesterol, triglycerides, and glucose, along with significantly elevated albumin and globulin concentrations. In terms of immune response, neutrophil counts were significantly higher in T4 and T5. Crucially, following a challenge with A. hydrophila, survival rates were higher in T4:90% and T5:85% compared with positive control’s meager 20% survival. Post-challenge hematology confirmed that groups T4 and T5 maintained an enhanced immune status. These findings suggest that combining medicinal plants extract with vaccine effectively enhances growth, immunity, and disease resistance in L. rohita, presenting an environment friendly alternative to traditional antibiotics in aquaculture. Full article

Automated milk feeders (AMFs) offer significant advantages in promoting natural feeding behavior in calves and reducing manual labor. With widespread use, the impact of AMF hygiene on calf health has attracted increasing research attention, as inadequate cleaning protocols may lead to bacterial accumulation on calf-contact surfaces and subsequent health risks for calves. This study aimed to quantify bacterial contamination on AMF surfaces, evaluate the cleaning efficacy of slightly acidic electrolyzed water (SAEW) compared to warm water and chemical disinfectants (n = two total samples), and optimize SAEW cleaning parameters using response surface methodology (RSM). Results revealed that bacterial loads on five high-frequency calf-contact surfaces ranged from 5.48 to 8.21 log₁₀ CFU/cm². SAEW at 60 mg/L achieved significantly higher cleaning efficacy than warm water and chemical disinfectants under field conditions (p < 0.01). Through RSM optimization (highly significant (p < 0.001)), the optimal SAEW cleaning parameters were determined as follows: cleaning time of 35 s, cleaning temperature of 78 °C, and available chlorine concentration (ACC) of 108 mg/L. Under the optimized parameters, bacterial and adenosine triphosphate (ATP) removal rates reached approximately 98%. These findings suggest that SAEW is a promising alternative sanitation agent for AMFs, provide preliminary parameters for rapid sanitation under the tested conditions, and hold the potential to support the standardized hygiene control of calf feeding equipment. Full article

Bedellia somnulentella (Lepidoptera: Bedelliidae) is an invasive, leaf-mining, and defoliating pest of sweet potatoes (Ipomoea batatas L.) that has recently established in Brazil. Its colonization and infestation levels in cultivated fields are influenced by the availability of wild Ipomoea species that sustain populations during off-season periods. The objective was to evaluate the biology and life history of B. somnulentella feeding on wild plants of the genus Ipomoea and on I. batatas cv. Beauregard. Vegetative and reproductive parts of Ipomoea plants were collected and cultivated, and the biology and life history of B. somnulentella were studied using twenty adult pairs of the insect per host plant in a climate-controlled room. The wild species Ipomoea hederifolia L., Ipomoea indica (Burm.f.) Merr., Ipomoea purpurea L., and cultivated I. batatas were used for the assays. The experiment followed a completely randomized design with ten replicates. Hatching, larval stages, prepupa, pupa, and adult phases were observed and recorded daily. Variations in the coloration of B. somnulentella larvae feeding on leaves of I. hederifolia, I. indica, and I. purpurea were observed. The survival and development of B. somnulentella were higher on I. batatas and I. hederifolia than on I. indica and I. purpurea, mainly during the larval and adult stages. The results provide information on infestation in alternative wild hosts and on biological aspects of B. somnulentella. Full article

The adoption of cost-effective feeding regimes is essential to maintain the growth and physiological status of lambs after weaning. The economic benefits of an alternative concentrate diet for Omani lambs were also assessed. Twenty male Omani lambs (12.31 ± 3.22 kg; 5 months old) were allocated to a commercial concentrate (control) or formulated concentrate group (n = 10/treatment) for 63 days. The formulated concentrate consisted of date palm fronds (29%), barley (20%), fish meal (12%), date syrup (10%), Moringa oleifera (28%), and salt (1%), with all ingredient proportions expressed on a dry matter (DM) basis. Growth performance, feed efficiency, hematological indices, serum biochemical parameters, and cost analyses were evaluated using individual lambs as the experimental unit. Growth performance, feed efficiency, hematological and serum biochemical parameters, and cost analyses were performed. Final body weight (BW), total weight gain (TWG), average daily gain (ADG), and feed conversion ratio (FCR) were similar between the groups (p > 0.05). The average daily feed intake was similar between groups (p = 0.321). However, the feed cost per kg of gain and feeding cost were lower (p < 0.001) by 63 and 58%, respectively, for the formulated concentrate group. Time × group interactions were observed for red blood cell parameters, with higher platelet counts (p < 0.001) and reticulocyte hemoglobin content (p = 0.018) in the formulated concentrate group. The serum biochemical parameters changed over time, with similar responses between the groups. Appropriate substitution of ingredients in lamb concentrate diets with agricultural by-products positively influenced economic efficiency. Full article

Finding a sustainable approach to rainbow trout aquaculture is very important. This study aimed to evaluate the effects of an experimental diet (D2) including artisanal fishery discards (whiting fish—Merlangius merlangus; tub gurnard—Chelidonichthys lucerne; horse mackerel—Trachurus trachurus) and feedstuffs from the fish farmer’s farmhouse (wheat and dehulled peas) in comparison to a control diet (D1) on the growth performance and fillet quality of rainbow trout—Oncorhynchus mykiss (initial mean body weight: 48 ± 3 g). In D2, fish oil was substituted with algal oil. The trial was performed in flow-through basins. An economic assessment was performed, considering the most important economic indicators, based on the cost of feed and the cost to obtain fish biomass, comparing D2 to D1. A final reduction in total ammonia nitrogen in D2 water (TAN; 0.28 ± 0.01 mg/L vs. 0.42 ± 0.03 mg/L for D2 and D1, respectively) was observed. No significant differences in growth performance were observed, although there was a slight difference in the Feed Conversion Rate. Use of algal oil as a dietary ingredient ensured high-quality omega-3 fatty acids in the fish fillets, with a significant improvement in the DHA content of D2 fish (1131.0 ± 1.8 mg/100 g) compared to their D1 counterparts (435.0 ± 0.5 mg/100 g). The economic analysis corroborates the benefit of using marine fish by-products as alternative protein sources for rainbow trout aquaculture, as the D2 feed has a lower formulation unitary cost (−15.4%) than the D1 feed, suggesting that in addition to their efficacy in growing fish, marine fish by-products are a valuable protein source from an economic standpoint. Since this experiment was implemented at the farm level, these outcomes suggest the diet can be realistically and sustainably applied in the European rainbow trout farming sector. Full article

In China, the substantial gap between domestic soybean supply and growing consumption necessitates large-scale soybean imports. The use of cultivated soybean (Glycine max) leaves as feed for the edible insect Clanis bilineata tsingtauica reduces crop yield, posing a threat to national soybean production security. To address this issue, this study evaluated wild soybean (Glycine soja) as a potential alternative feed source. Comparative analyses examined the nutritional and anti-nutritional properties of G. max (cv. Qihuang34) and a laboratory-preserved G. soja germplasm, together with their effects on larval growth performance, nutritional composition, and associated microbiota. G. soja leaves exhibited significantly higher crude fat (5.61% vs. 2.17%), ash (11.07% vs. 9.62%), neutral detergent fiber (23.75% vs. 21.00%), calcium (4.05 g/kg vs. 3.41 g/kg), and phosphorus (2.52 g/kg vs. 2.38 g/kg) than G. max leaves, along with lower trypsin inhibitor levels (p < 0.01) despite higher phytic acid content (p < 0.05). Fifth-instar larvae reared on G. soja leaves showed a 12.9% greater body weight (6.846 g vs. 6.066 g), higher crude protein (672.14 g/kg vs. 555.02 g/kg), total soluble sugar (21.27 mg/g vs. 8.96 mg/g), and soluble protein (26.35 mg/g vs. 24.71 mg/g), but lower crude fat (187.44 g/kg vs. 205.82 g/kg, p < 0.05). 16S rRNA sequencing revealed distinct phyllosphere microbial communities, with G. soja enriched in diverse taxa (e.g., Bacteroidota, Proteobacteria) and G. max dominated by Firmicutes. Corresponding differences were observed in larval gut microbiota, and positive correlations suggested potential microbial transfer from G. soja leaves to larval guts. Overall, G. soja represents a promising alternative feed source for C. bilineata, reducing competition with soybean grain production and supporting sustainable insect farming. Full article

The environmental impacts from fossil fuel use have accelerated the global transition to sustainable energy sources. Hydrogen has become a promising alternative due to its high energy density and clean combustion. However, hydrogen production streams are frequently contaminated with methane, which needs efficient, durable, and cost-effective purification technologies such as pressure swing adsorption (PSA). The present study provides a comparative evaluation of biomass-derived activated carbons and a commercial activated carbon for hydrogen–methane separation. High-surface-area activated carbons were synthesized from sustainable pine and birch precursors via chemical activation using potassium hydroxide (KOH, impregnation ratio 3:1) at 800 °C. Their dynamic adsorption performance was systematically assessed in a fixed-bed setup under a PSA system operating at pressures of 25, 35, and 50 bar, using a of hydrogen–methane gas mixture, where methane feed concentrations ranging from 10 to 30 vol%. This work focuses on the behavior of the adsorbent material and does not constitute a complete PSA process evaluation. The biomass-derived activated carbons showed well-developed textural characteristics, with specific surface areas up to 1416 m² g⁻¹, which exceeded that of the commercial reference material (1023 m² g⁻¹). This improved pore structure was reflected in their adsorption behavior at an operating pressure of 50 bar; the birch-derived carbon achieved a methane uptake of 10.5 mol kg⁻¹, more than twice the capacity of 5.30 mol kg⁻¹ measured for the commercial adsorbent. Beyond initial adsorption capacity, the study emphasizes operational durability and reusability. Cyclic adsorption–desorption experiments, supported by Raman spectroscopy, revealed pronounced structural changes in the commercial activated carbon under repeated operational stress, as indicated by an increase in the I_D/I_G ratio from 1.08 to 1.24. In contrast, the biomass-derived activated carbons preserved their morphological integrity and adsorption efficiency over successive cycles. These findings demonstrate that pine- and birch-derived activated carbons are not only sustainable alternatives but also operationally stable adsorbents capable for hydrogen purification processes. Full article

Using alternative energy sources for animal feed, such as hybrid corn varieties rather than genetically modified ones, is important. Therefore, the objective of this work was to assess the effects of supplementation at the end of gestation with Mexican Puma hybrid corn grain on productive and behavioral parameters in sheep. Twenty Columbia multiparous ewes were used; along with their diet, they were provided 600 g/animal/day of cracked corn during the last 20 days of pregnancy and the first week of lactation. The animals were divided into two groups: one fed commercial cracked corn (n = 11) and the other Mexican Tlaoli Puma hybrid cracked corn (n = 9). The productive parameters evaluated in the mother were: body weight, body condition score (BCS), feed intake, weight change, glucose, and ketone body levels, as well as the estimated quality of milk using Brix refractometer values on days 15 and 30 of lactation. In lambs, their rectal and external temperature was measured 2 h after birth, while their weight was measured 2 h after birth and every week until week 6 postpartum. Behavioral parameters were measured in the first two hours postpartum, including the maternal latency of cleaning the offspring, duration of the first cleaning episode, the lamb’s latencies of standing and nursing, and vocalizations in mother and lamb. Weight, BCS and weight change were not affected by the group but were affected by time; these parameters increased at the end of gestation and decreased significantly after delivery (p < 0.05). Ketone body levels were not affected by group or time (p > 0.05) and remained at low values. Glucose levels were not affected by the group but were affected by time; they increased significantly after birth (p < 0.05). Feed intake was similar in both groups (p > 0.05) and decreased as parturition approached (p < 0.05). The estimated milk quality was not affected by the group, nor by the time (p > 0.05). Mothers in both groups began cleaning their offspring within the first three minutes after giving birth and emitted a similar frequency of vocalizations (p > 0.05). However, mothers in the commercial maize group had a longer cleaning episode than those in the hybrid maize group (p < 0.05). The lambs in both groups stood up within the first half hour of birth, suckled before one hour after birth and emitted a similar number of vocalizations (p > 0.05). Temperatures and lamb weight were similar in both groups (p > 0.05); however, lamb weight increased as they aged (p < 0.05). It is concluded that supplementing sheep at the end of gestation with Puma hybrid Mexican corn grain can yield similar productive and behavioral benefits as supplementing with commercial grain. Full article

Pseudomonas aeruginosa is a widespread pathogen that causes acute and chronic diseases in various organisms, including humans. Treating this antibiotic-resistant bacterium is challenging, so alternative or supplementary treatment strategies are desirable. Six novel bacteriophages specific to P. aeruginosa were isolated and classified into the genera Septimatrevirus, Kochitakasuvirus, Bruynoghevirus, and a new, unnamed genera related to Napahavirus, and Kantovirus. Their genomes were annotated and further characterized. We used the Drosophila melanogaster insect model to predict the efficacy of the phages in terms of their curative function on other organisms. Flies were chronically infected by feeding them bacteria and were subsequently treated with individual bacteriophages. The results of the Kaplan–Meier survival test revealed differences in phage efficacy and supported the hypothesis that the phages had a curative effect. These mentioned phages extended the flies’ lifespan. Full article

This study aimed to develop a multi-bioactive composite plant extract as an alternative to dietary antibiotics for application in animal production. Five plant materials were initially selected from 23 candidate plants via in vitro antibacterial, antioxidant, and anti-inflammatory screening, and formulated into three candidate extracts (C1, C2, C3) by orthogonal design, with respective dominant activities and moderate the other activities. Three feeding trials in mice demonstrated that administration of 1000 mg/kg C1 or C2 caused no adverse effects on hematological parameters or organ indexes. Supplementation with 250 mg/kg C1 or 125 mg/kg C2 significantly increased body weight gain and feed intake, reduced the feed-to-gain ratio, and modulated gut microbiota composition. In LPS-challenged mice, C1 and C2 restored jejunal villus height and crypt depth, downregulated the gene expression of TLR4, TNF-α, NF-κB, and IL-1β, and increased hepatic T-AOC activity while decreasing MDA content. Furthermore, a feeding trial in piglets demonstrated that dietary supplementation with 200 mg/kg C2 achieved growth performance comparable to that of conventional antibiotic supplementation, highlighting its potential as a substitute for feed antibiotics. In conclusion, this study has developed a new multi-bioactive composite plant extract that may serve as a promising alternative to feed antibiotics. Full article