Search Results (57)

In recent decades, European countries have seen a substantial increase in home cultivation of cannabis. In France, the prevalence of cannabis use continues to increase despite its possession, sale, and cultivation being strictly illegal. The present study aimed to describe the profile and motivations of people in France who cultivate cannabis at home. We separately analyzed data from two convenience samples of people who use cannabis daily in France, based on two online cross-sectional surveys. In the first analysis (N = 3840), we used a multivariable logistic regression model to assess factors associated with home cultivation as the main source of cannabis supply. In the second analysis (N = 574), we described participants’ motivations for home cultivation and their cultivation patterns. In the two samples, 11% and 16% reported home cultivation as their main source of supply, respectively. Age, male gender, stable housing, living with a partner, consuming cannabis in herbal form, smoking joints with little or no tobacco, smoking cannabis from a bong or pipe, non-smoking modes of cannabis administration, and using cannabis exclusively for therapeutic reasons were all positively associated with home cultivation, while urban area of residence and at-risk alcohol use were negatively associated. The main reason reported for home cultivation was to manage quality. Few reported selling some of their crop, and most were self-sufficient. Finally, we interpret this practice as a personal response to cannabis prohibition and the unregulated market. Accordingly, possible harm reduction strategies are discussed. Full article

Fear is a critical welfare concern in laying hens. Fearful behaviors in domestic chickens are influenced by both genetic and environmental factors, contributing to individual differences in stress responses. Tonic immobility (TI) duration is widely recognized as a reliable indicator of fear levels. The hippocampus, a critical brain region for emotional states, plays a pivotal role in associating fearful experiences with specific stimuli, enabling adaptive behavioral responses. This study investigated hippocampal histological characteristics and transcriptomic profiles in laying hens with different fear responses categorized based on TI duration. A total of 80 native Lindian hens (75 weeks old) were individually housed in modified conventional cages. At 76 weeks of age, hens exhibiting the longest and shortest TI durations were classified into the high-fear (TH) and low-fear (TL) groups, respectively. Whole hemibrains were collected for histological and immunohistochemical analyses, while hippocampal tissues underwent transcriptome sequencing. The results showed a significant reduction in Nissl body counts in hippocampal neurons of high-fear hens (p < 0.05), suggesting potential neuronal damage or functional impairment. Transcriptomic analysis revealed 365 differentially expressed genes (DEGs) between two groups, with 277 upregulated and 88 downregulated genes in TH chickens. KEGG pathway enrichment analysis identified seven significantly associated pathways (p < 0.01), including retinol metabolism, vitamin B6 metabolism, and nicotinate and nicotinamide metabolism, all of which are crucial for neuronal function and immune regulation. In addition, a significant increase in DCX protein expression (p < 0.05) and a decrease in c-Fos protein expression (p < 0.05) was noted in in high-fear hens, whereas PCNA levels remained unchanged (p > 0.05) under immunohistochemical validation. The neuronal alterations observed in high fear individuals suggest neural damage, while transcriptomic variations point to potential disruptions in neurogenesis, synaptic signaling, and stress-related pathways. Collectively, these results provide novel insights into the neurobiological basis of fear regulation in laying hens and may have implications for poultry welfare and management strategies. Full article

Heat waves affect the health and quality of life of older adults, particularly in urban environments. However, there is limited understanding of how extreme temperatures influence their mobility. This research aims to understand the pedestrian mobility patterns of older adults during heat waves in Madrid, analyzing environmental and sociodemographic factors that condition such mobility. Geospatial data from the mobile phones of individuals aged 65 and older were analyzed, along with information on population, housing, urban density, green areas, and facilities during July 2022. Multiple linear regression models and Moran’s I spatial autocorrelation were applied. The results indicate that pedestrian mobility among older adults decreased by 7.3% during the hottest hours, with more pronounced reductions in disadvantaged districts and areas with limited access to urban services. The availability of climate shelters and health centers positively influenced mobility, while areas with a lower coverage of urban services experienced greater declines. At the district level, inequalities in the availability of urban infrastructure may exacerbate the vulnerability of older adults to extreme heat. The findings underscore the need for urban policies that promote equity in access to infrastructure and services that mitigate the effects of extreme heat, especially in disadvantaged areas. Full article

Plastic waste reduction has become a global priority, with consumer engagement playing a crucial role in the success of sustainability initiatives. This study examines the demographic and behavioral determinants of consumer engagement in plastic tableware reduction. Using survey data from Hong Kong residents and a Multivariate Analysis of Variance (MANOVA) approach, this study analyzes how age, gender, education, income, housing type, order frequency, opt-out effectiveness, and their interactions influence the four dimensions of engagement, namely plastic tableware opt-out behavior, support for government policies, support for plastic-free restaurants, and price sensitivity. The results indicate that age significantly affects plastic tableware reduction engagement, with order frequency and opt-out effectiveness moderating the effects of age and education. The study contributes to the literature by providing empirical insights into consumer-driven sustainability efforts and the role of behavioral factors in shaping engagement in plastic waste reduction. These findings offer valuable implications for policymakers and businesses promoting sustainable consumption practices. Full article

Aging residential buildings in urban areas require effective thermal insulation to enhance energy efficiency and indoor comfort. In Bosnia and Herzegovina (BiH), expanded polystyrene (EPS) is the most commonly used insulation material due to its affordability, despite concerns regarding its flammability and environmental impact. While regulatory changes since 2019 have recommended rock wool for high-rise buildings, the absence of binding fire safety regulations has allowed the continued use of EPS, often driven by financial constraints. This study examines energy efficiency refurbishments in Sarajevo’s high-rise residential buildings and analyze the implications of the partial implementation of recommended measures. Using case studies, surveys, and expert interviews, this research identifies key challenges, such as limited funding, fragmented renovations, and inconsistent coordination between stakeholders. The findings indicate that facade insulation is often prioritized over comprehensive upgrades, including window replacement and heating system improvements, leading to suboptimal energy savings and minimal cost reductions for residents. Additionally, the complexity of multi-apartment ownership structures hinders uniform improvements in energy efficiency. Despite these challenges, property values tend to increase after renovation, highlighting the long-term financial benefits. To maximize energy savings and ensure sustainable urban housing, stronger interdisciplinary collaboration, improved funding mechanisms, and adherence to fire-safety standards are necessary. These measures would enhance the effectiveness of renovations and support long-term energy efficiency strategies. Full article

Background: Childhood is a crucial period for brain development, and short-term juvenile stress has demonstrated long-lasting effects on cognitive and cellular functions in the hippocampus. However, the influence of such stress on the brain’s overall network remains unclear. Methods: In this study, we employed functional magnetic resonance imaging (fMRI) to explore the effects of transient wild stress on juvenile male rats. Pregnant rats were purchased and housed in a specific pathogen-free (SPF) environment, with pups separated by sex on postnatal day 21 (PD21). From PD27 to PD29, male rats were subjected to transient wild stress, which included forced swimming, elevated platform exposure, and restraint stress. Following stress exposure, all animals were carefully maintained and scanned at 42 days of age (PD42) using fMRI. Structural analysis was performed using voxel-based morphometry (VBM) to assess changes in gray matter volume, while functional activity was evaluated through regional homogeneity (ReHo) and voxel-wise functional connectivity. Results: The results showed significant reductions in gray matter volume in several brain regions in the stress group, including the periaqueductal gray (PAG), entorhinal cortex (Ent), and dentate gyrus (DG). In terms of functional activity, cortical regions, particularly the primary somatosensory areas, exhibited decreased activity, whereas increased activity was observed in the PAG, DG, and medulla. Furthermore, functional connectivity analysis revealed a significant reduction in connectivity between the DG and entorhinal cortex, while the DG-PAG connectivity was significantly enhanced. Conclusions: These findings suggest that juvenile stress leads to profound alterations in both brain structure and function, potentially disrupting emotional regulation and memory processing by affecting the development and connectivity of key brain regions. Full article

The global commitment to ending hunger by 2030 has driven Colombia to align its Sustainable Development Goals (SDGs) toward reducing food waste and ensuring access to safe, nutritious food. A critical need is monitoring cumulative temperatures across food supply networks, prioritizing products over transport or storage infrastructure. This study introduces a Functional Time–Temperature Indicator (TTI) using nanodispersions of silver (Ag) and gold (Au) nanoparticles housed in 3D-printed plant-based resin containers. Nanoparticles were synthesized via three methods: in situ reduction (AgNPs), seed-based thermal synthesis (AgTNPs), and pulsed laser ablation in liquid (AuNPs). The TTIs operate through three colorimetric mechanisms: NP concentration, geometry changes, and agglomeration. At 4 °C, AgNPs and AgTNPs maintained stable color, while at 22 °C, they exhibited significant changes, with AgNPs reaching 252% variation within 5 h. AuNPs responded at lower temperatures, showing up to 27% variation. Containers enabled effective nanodispersion incorporation due to their thermal and optical properties. AgTNP-based TTIs demonstrated the most noticeable changes at 22 °C, with a total color difference ($\Delta E$) of 39.9, easily detectable by observers. These TTIs provide robust solutions for continuous cold chain monitoring, enhancing food safety and preserving quality throughout the supply chain. Full article

The use of agro-industrial by-products in livestock nutrition is of increased interest to the feed industry. Some of the by-products are rich in bioactive compounds that could lead to an increase in the quality of animal meat products. This study aimed to evaluate the effects of dietary incorporation of grape pomace (GP), a by-product rich in polyphenols, on growth and on the oxidation status and the fatty acid profile of rabbit meat. Forty-eight male rabbits (Oryctolagus cuniculus) (aged 55 ± 3 d and ca 2.3 kg) divided into three groups of 16 were used in the trial. The rabbits were housed in pairs in wire-mesh cages (26 × 46 × 35 cm) giving n = 8 for each diet treatment. The first group was fed a basal diet (CTR) (ca 18% protein and ca 4% fat), the second was given a basal diet supplemented with 5 g GP per head per day (low-GP) and the third group was fed a basal diet supplemented with 10 g GP per head per day (high-GP). The trial lasted three weeks, and the animals were weighed weekly. The rabbits were slaughtered, and the weight and the pH of the carcasses was measured immediately after slaughter and after 24 h of cooling at 4 °C. The pH of the stomach and cecum was measured. A sample of Longissimus Dorsi (LD) was collected and stored at −80 °C for the analysis of proximate composition, thiobarbituric acid reactive substances (TBARs) and fatty acid profile (FA). The diet did not influence the growth or the pH of the stomach and cecum of the rabbits. The chemical composition of the meat was similar in all groups, with a mean protein of 22.3% and fat of 0.95%. The fatty acid profiles of the LD were not influenced by the diet, and the most abundant FAs were C16:0, C18:2n6 and C18:1c9. Feeding GP to the rabbits gave a linear decrease of TBARs in the meat, indicating a reduction in oxidative stress. The TBARs’ reduction was 60% in the meat of the rabbits given high-GP compared to CTR. In conclusion, GP could be used in rabbit diets without any negative influence on meat quality but with an increase in oxidation stability that could enhance meat shelf-life. Full article

The intensive raising model for meat duck production has widely adopted cascading cages. However, investigations on low-protein diets for Pekin ducks under this model are scarce. Thus, a 3 × 6 factorial experiment was conducted using six dietary crude protein (CP) levels (17.61%, 16.25%, 15.34%, 14.55%, 13.38%, and 12.64%, analyzed) and three cage layers (lower, middle, and upper). The study aimed to examine the effects of dietary CP reduction with crystalline amino acid supplementation on growth performance, carcass traits, and apparent nutrient digestibility in growing Pekin ducks from 21 to 42 days of age housed at different cage layers. A total of 1620 21-day-old Pekin ducks were randomly divided into 18 groups, each with six replicates of 15 ducks per replicate. Ducks were distributed across different cage layers and were fed one of six experimental diets, respectively, each varying in dietary CP levels but maintaining similar dietary energy and amino acid profiles. The results showed that the reduction in dietary CP levels affected growth performance (p < 0.05), while weight gain, feed intake, and feed/gain of Pekin ducks had no difference among 17.61%, 16.25%, and 15.34% CP groups (p > 0.05). As dietary CP decreased, abdominal fat yield increased (p < 0.05), but breast and leg meat yields were unaffected (p > 0.05). CP intake, nitrogen intake, and nitrogen excretion decreased linearly (p < 0.05) with decreasing dietary CP levels, while apparent nutrient digestibility in Pekin ducks increased proportionally (p < 0.05). Additionally, body weight, weight gain, feed intake, CP intake, nitrogen intake, and nitrogen excretion of Pekin ducks decreased (p < 0.05) due to different cage layers, while carcass characteristics remained unaffected by cage layer (p > 0.05). In summary, it is feasible to reduce the dietary CP level with crystalline amino acid supplementation in growing Pekin ducks from 21 to 42 days of age housed in cascading cages, while the cage layers affected growth performance of ducks. Based on broken-line models, it was determined that the optimal dietary CP requirement for achieving maximum weight gain and feed efficiency in growing Pekin ducks is approximately 15%. Full article

Proliferative enteropathy (PE) is characterized by diarrhea and reduced weight gains in growing pigs and intestinal hemorrhage in finishers. Vaccination, antibiotic medication, and improved hygiene can control PE, but their efficacy depends upon the epidemiology of PE. This study monitored the timing and severity of PE in 84 commercial pens across seven treatments, including disinfection, vaccination, no treatment, medication with olaquindox (50, 25 and 12.5 ppm), and combined disinfection and vaccination. Vaccination with or without lime disinfection suppressed clinical signs of PE and reduced the number of excreted L. intracellularis relative to untreated pigs housed in cleaned or cleaned and disinfected pens between 9 and 17 weeks of age. Continuous olaquindox mediation to 17 weeks of age prevented L. intracellularis infection, leaving finisher pigs naïve. These finisher pigs suffered an outbreak of hemorrhagic enteropathy with significant reductions in weight gain, feed intake, and mortalities of 4.6%. Over the 13 week grow/finish period, vaccinated pigs housed in disinfected pens showed significantly higher weight gain and feed intake relative to all other treatments, equating to a weight gain difference of between 3.6 and 3.9 kg per pig. Monitoring the immune response and fecal excretion of L. intracellularis in pens of pigs enabled effective PE control strategies to be evaluated on the farm. Full article

We undertook a retrospective study to compare the quality of care delivered to a cohort of newly diagnosed adults with colon, rectal or anal cancer during the early phase of COVID-19 (02/20–12/20) relative to the same period in the year prior (the comparator cohort), and examine the impact of the pandemic on 2-year disease progression and all-cause mortality. We observed poorer performance on a number of quality measures, such as approximately three times as many patients in the COVID-19 cohort experienced 30-day post-surgical readmission (10.5% vs. 3.6%; SD:0.27). Despite these differences, we observed no statistically significant adjusted associations between COVID-19 and time to either all-cause mortality (HR: 0.88, 95% CI: 0.61–1.27, p = 0.50) or disease progression (HR: 1.16, 95% CI: 0.82–1.64, p = 0.41). However, there was a substantial reduction in new patient consults during the early phase of COVID-19 (12.2% decrease), which appeared to disproportionally impact patients who traditionally experience sociodemographic disparities in access to care, given that the COVID-19 cohort skewed younger and there were fewer patients from neighborhoods with the highest Housing and Dwelling, ands Age and Labour Force marginalization quintiles. Future work is needed to understand the more downstream effects of COVID-19 related changes on cancer care to inform planning for future disruptions in care. Full article

Malta faces a significant challenge in reducing carbon emissions, with energy consumption in its 153,100 occupied residences contributing to 30% of CO₂ emissions. This study focuses on a sample of an 1870s, 80 sq-m footprint, three-story residence, emblematic of similar properties facing marketability issues due to age, structure, and maintenance. The objective is to assess a techno-economic energy and CO₂ abatement framework, including advanced lighting devices, appliances, photovoltaics, wind turbines, energy storage, and vehicle-to-grid possibilities. The research evaluates comfortability and calculates potential 25-year kWh reduction and cost savings for each measure. The findings demonstrate the feasibility of implementing diversified renewable and alternative energy sources in such residences. Over 25 years, approximately 250 MWh of energy could be mitigated, leading to a reduction of approximately 140 metric tons of carbon dioxide. The study emphasizes the importance of housing stock efficiency in both new construction and retrofitting, focusing on building performance for health, comfort, and living standards. While most systems are viable, further research is needed for system-wide strategy implementation, particularly in areas like energy storage and wind turbine solutions. The study concludes that adopting emerging technologies could be advantageous in minimizing system costs through innovative building-integrated designs. Full article

This paper’s main objective is to assess the impacts of the ban on cages for housing laying hens, planned by the European Commission to raise animal welfare standards beyond the level set in the current legislation. The farm-level economic assessments of the ban were carried out in three stages: farm surveys and expert consultations, farm-level analyses, and aggregation to the EU-27 egg production sector. Four scenarios were constructed. All financial estimates were conducted with fixed prices from the year 2021 for which the reference scenario was built. Alternative hen-housing systems were barn (Voliera), free range, and organic. Until now, more than 50% of laying hens in the EU have already been transferred to alternative systems. The remaining part is subject to the transition. The basic assumptions included a reduction in yields due to the required lower densities and specifics of the production systems. A factor strongly differentiating the scenarios is likelihood of exists form the sector, as declared in the survey by many farmers, mainly those reaching retirement age without successors and keeping relatively small flocks of hens. The introduction of the ban will cause a decrease in egg production, varying between the scenarios. Substantial investments will be required within the range of 2–3.2 billion EUR, depending on the scenario. Full article

The primary aim of this research was to assess the impact of supplementing fish oil into the diet of pregnant Awassi ewes on various aspects of the dam’s productive performance, offspring birth weight, colostrum yield and quality, milk production and composition, postpartum offspring performance, and the composition of fatty acids in milk. In this study, two different fat sources, namely palm oil (PO) and fish oil (FO), were utilized, with both being included at a rate of 2.4% of dietary dry matter (DM) during the initial 65 days of gestation (early gestation stage), and then at 2.1% DM from day 65 of gestation until lambing (late gestation stage). The study subjects were Awassi ewes with a body weight (BW) averaging 57 ± 1.5 kg and an age of 3.5 ± 1.2 years. These ewes were randomly assigned to one of four dietary treatments: (i) a diet containing only palm oil from day 0 to day 150 of pregnancy (PO, n = 15); (ii) a diet containing palm oil from day 0 to day 65 of pregnancy, followed by a diet containing fish oil from day 66 to day 150 of pregnancy (POFO, n = 16); (iii) a diet containing fish oil from day 0 to day 65 of pregnancy, followed by a diet containing palm oil from day 66 to day 150 of pregnancy (FOPO, n = 16); (iv) a diet containing only fish oil from day 0 to day 150 of pregnancy (FO, n = 16). Within each treatment, ewes were housed in three replicates, with each replicate further divided into three subgroups. The first two subgroups each contained eight ewes, while the third subgroup contained nine ewes. In addition, each treatment contained 10 primiparous and 15 multiparous ewes. The results indicated that the POFO treatment led to significant increases (p < 0.05) in birth weight, gestation length, and colostrum IgG concentration. However, it also resulted in a decrease (p < 0.001) in colostrum production and a reduction (p < 0.001) in the percentage of milk fat and milk protein. Furthermore, the milk from the POFO treatment exhibited higher levels of polyunsaturated fatty acids (PUFAs) (p < 0.001) compared to the PO and FOPO treatments, while the milk saturated fatty acids (SFA) were lower (p < 0.001). In conclusion, the addition of fish oil at a rate of 2.1% DM during the late pregnancy period showed promise for enhancing birth weight, colostrum IgG concentration, and the PUFA content in the milk. Full article

A total of 29.5 million people aged 12 and older met the diagnostic criteria for alcohol use disorder (AUD) in the United States in 2021, which presents a significant social and economic burden to modern society. Impaired nutritional status has been frequently documented in patients with AUDs and could contribute to escalated alcohol consumption and behavioral impairments commonly observed in AUD. Interestingly, increased highly palatable food intake during recovery has been reported in patients with AUD, suggesting the importance of understanding the relationship between palatable food and problematic alcohol drinking. We have previously shown that patterned feeding of a palatable diet attenuated alcohol drinking in Long Evans rats. The present study evaluated the impact of patterned feeding on high and low alcohol drinking. Individually housed male high-drinking (P), moderate-drinking (Long Evans), and low-drinking (Wistar) rats received intermittent access (24 h, Tuesdays, and Thursdays) to a nutritionally complete high-fat diet (Int-HFD) or standard chow (controls). Normal chow and water were available ad libitum to all groups of rats. Intermittent HFD access induced a feeding pattern in which the Int-HFD group of rats escalated their caloric intake on Tuesdays and Thursdays. Two weeks of Int-HFD pre-exposure preceded any alcohol access, after which all rats were given unsweetened alcohol (20% v/v) in their home cages via a two-bottle choice paradigm of voluntary alcohol drinking. Alcohol was available for 24 h on chow-only days (Mondays, Wednesdays, and Fridays) while Int-HFD feeding continued. Long Evans rats receiving the Int-HFD displayed a significant ~50% reduction in alcohol drinking when compared to controls. The Int-HFD group of P rats also reduced their alcohol intake significantly (p < 0.05) by ~20%, in comparison to respective controls. Interestingly, alcohol drinking in Wistar rats was not affected (p > 0.05) by intermittent HFD exposure. These data highlight rat strain-specific differences in alcohol intake following patterned feeding of a palatable diet and identify Long Evans rats as an ideal model to evaluate the impact of palatable diet on alcohol drinking. Full article