Search Results (52)

The house fly, Musca domestica L., is a pest of great medical and agricultural importance, serving as a vector for various diseases and undermining the quality of agricultural products. Traditionally, synthetic insecticides have been the primary means of control; however, their efficacy has declined over time, and they are now less preferred due to their safety and environmental concerns. This study evaluated the insecticidal and repellent properties of essential oils from Eucalyptus globulus, Foeniculum vulgare and Salvia officinalis against M. domestica. All EOs exhibited insecticidal activity: eucalyptus achieved 100% fumigant mortality at 34 µL L⁻¹ air and showed the lowest LC₅₀ (18.1 µL L⁻¹ air), while fennel and sage required 50 µL L⁻¹ air. In contrast, fennel showed the highest contact toxicity (100% mortality at 150 µL L⁻¹). Repellency exceeded 87% for all EOs, with sage being the most repellent at the lowest concentration tested (94% at 5 µL L⁻¹). These results highlight the potential use of essential oils and their constituents as environmentally friendly alternatives for the control of M. domestica. However, further field validation and studies on individual components and their synergistic combinations are needed to understand their efficacy and fully optimize their use. Full article

New intervention methods and product formulations are needed to better control pyrethroid-resistant Aedes aegypti populations and mitigate the risk of mosquito-borne disease. ReMoa Tri is a novel adulticidal space spray that utilizes a different mode of action than the commonly used adulticides: pyrethroids and organophosphates. As a triple-action space spray, ReMoa Tri combines three components: Fenpropathrin, a mixed-type I/II pyrethroid; abamectin, a macrocyclic lactone; and C8910, a patented fatty acid chain. Prior studies performed by Collier Mosquito Control District showed that ReMoa Tri is effective at controlling type I pyrethroid-resistant Ae. aegypti mosquitoes. To further validate these results and the performance of ReMoa Tri, we conducted a semi-field evaluation using ground and aerial ULV (ultra-low volume) applications with field-caught deltamethrin-resistant Ae. aegypti and a susceptible Ae. aegypti laboratory strain. Ground evaluations tested ReMoa Tri and a type II pyrethroid-based product, DeltaGard. While ReMoa Tri was equally effective against Collier’s deltamethrin-resistant Ae. aegypti and the susceptible laboratory strain, DeltaGard was effective against both strains, with reduced efficacy at farther distances. Similarly, aerial evaluations also showed that ReMoa Tri was equally effective against Collier’s deltamethrin-resistant Ae. aegypti strain and susceptible laboratory strain. This study further confirms ReMoa Tri’s potential as an effective alternative to pyrethroid-based adulticides, both in ground and aerial applications, for managing pyrethroid-resistant Ae. aegypti. Full article

Aedes albopictus has established populations in several European countries with a sustained spreading pattern through the continent. This invasive mosquito is a public health threat due to its vector competence for multiple arboviruses. Notably, the peri-domestic habits of this hematophagous insect greatly diminish the efficacy of regular control activities, as individuals may harbor in private areas. The oviposition behavior can be exploited for targeting adults and immature stages through different types of traps. An experimental integrated control program, which included a community-based mass trapping intervention in private areas, control of public street-catch basins, and an educational campaign, was developed in an infested residential area in Valencia (Eastern Spain). Focusing on mass trapping, participating residents deployed traps belonging to three modes of action in their gardens during the mosquito season. A total of 1028 families participated in the project, and 2884 traps were deployed. The study sector where adult lethal ovitraps were used showed the lowest adult collections, and residents living in this sector reported the highest satisfaction rates in a perception survey. The mass deployment through a community-based approach of the adulticidal oviposition trap type appears to be a promising tool for controlling Ae. albopictus in residential areas. Full article

Although the present use of pesticides in plant protection has limited the occurrence and development of plant diseases and pests, resistance to pesticides and their environmental and health hazards indicates an urgent need for new active ingredients in plant protection products. Recently synthesized coumarin-1,2,4-triazole hybrid compounds have been proven effective against plant pathogenic fungi and safe for soil-beneficial bacteria. Drosophila melanogaster, the common fruit fly, has been used as a model organism for scientific research. Additionally, it is considered a pest since it damages fruits and serves as a carrier for various plant diseases. On the contrary, Orius laevigatus is a beneficial true bug that biologically controls harmful arthropods in agricultural production. In the present study, we performed an adulticidal bioassay against D. melanogaster and O. laevigatus using coumarin-1,2,4-triazole hybrids. Quantitative structure–activity relationship studies (QSARs) and in silico ecotoxicity evaluation elucidated the structural features underlying the compounds’ insecticidal activity. The derivative of 4-methylcoumarin-1,2,4-triazole with a 3-bromophenyl group showed great insecticidal potential. A molecular docking study indicated that the most active compound probably binds to glutamate-gated chloride channels. Full article

The overuse of synthetic pesticides has triggered resistance in insect pests and caused severe environmental impacts, emphasizing the urgent need for sustainable alternatives in Integrated Pest Management (IPM). This study aimed to biosynthesize and characterize chitosan-coated silver nanoparticles (AgChNPs) using Galega officinalis leaf extract and evaluate their insecticidal effects against Drosophila suzukii (Diptera: Drosophilidae), a key pest of fruit crops worldwide. The biosynthesized AgChNPs (257.2 nm) were polydisperse, crystalline, and stable, as confirmed by UV-vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). AgChNPs exhibited strong toxicity across multiple developmental stages. Combined larvicidal and pupicidal activity reached 48.3% and 73.3% at 500 and 1000 ppm, respectively, significantly affecting immature stages. As a consequence, adult emergence declined to 46.7%, 51.7%, and 26.7% at 250, 500, and 1000 ppm. Among emerged adults, 71.7% displayed sublethal effects, with 62.8% showing morphological malformations (deformed wings, dehydration) and 37.2% presenting cuticle demelanization. Adulticidal bioassays revealed progressive mortality over 48 h, with 96% mortality at 1000 ppm. Overall, AgChNPs caused acute and chronic toxicity, reduced adult emergence, and induced severe morphological alterations, demonstrating their potential as a sustainable nanotechnological tool for effective pest control within IPM programs. Full article

Lymphatic filariasis (LF) continues to impact 657 million individuals worldwide, resulting in lifelong and chronic impairment. The prevalent anti-filarial medications—DEC, albendazole, and ivermectin—exhibit limited adulticidal efficacy. Despite ongoing LF eradication programs, novel therapeutic strategies are essential for effective control. This study examines the mechanism of action of Ocimum sanctum on the filarial parasites Setaria cervi via a synergistic biochemical and proteomics methodology. The ethanolic extract of Ocimum sanctum (EOS) demonstrated potential anti-filarial action in the MTT reduction experiment, with an LC₅₀ value of 197.24 µg/mL. After EOS treatment, an elevation in lipid peroxidation (51.92%), protein carbonylation (48.99%), and NADPH oxidase (88.88%) activity, along with a reduction in glutathione (GSH) (−39.23%), glutathione reductase (GR) (−60.17%), and glutathione S transferase (GST) (−50.48%) activity, was observed. The 2D gel electrophoresis identified 20 decreased and 11 increased protein spots in the EOS-treated parasites relative to the control group. Additionally, in drug docking analysis, the EOS bioactive substances ursolic acid, rutin, and rosmarinic acid show a significant binding affinity with the principal differentially expressed proteins. This paper demonstrates, for the first time, that the anti-filarial efficacy of EOS is primarily facilitated by its impact on energy metabolism, antioxidant mechanisms, and stress response systems of the parasites. Full article

The past decade has witnessed an increase in scientific publications investigating the potential of essential oils as alternatives to synthetic pesticides for the control of plant and animal pests and disease vectors. The essential oil of Schinus molle has been the subject of various studies aimed at describing its insecticidal, acaricidal, and repellent properties. S. molle, although native to South America, is also widely distributed in countries bordering the Mediterranean. The objective of this systematic review was to analyze existing information on S. molle essential oil as a raw material for novel biopesticides and nanobiopesticides. We searched articles from PubMed, Scopus, and MDPI databases, and from 194 reports, we critically selected 33 articles produced between 2005 and 2024, representing all of the studies that aimed to evaluate the properties of the essential oil of this species as an insecticide, acaricide, and pesticide. The chemical composition varies significantly depending on origin, although two chemotypes associated with α-phellandrene and limonene appear to emerge. Data on adulticide activity and repellency are promising, and there are preliminary studies of microencapsulated forms of the essential oil that represent contemporary research trends currently under investigation. Furthermore, S. molle essential oil appears to demonstrate acetylcholinesterase inhibition effects that warrant further investigation. Finally, in this review, we have highlighted the potential of S. molle essential oil as a biopesticide, emphasizing the need to progress from a preliminary study phase to research conducted in application contexts. The conclusions of this review indicate future research trends aimed at the development of commercial products for organic and regenerative agriculture. Full article

Aedes aegypti is a major arboviral disease vector and is therefore a critical target for control by public health programs. Early eradication efforts have shown that Ae. aegypti can rapidly build insecticide resistance, and, now, resistance to pyrethroids, the major class of adulticides used for operational control, is common in many populations. A major contributor to this resistance is a group of knockdown resistance (kdr) SNPs that normally exist in distinct ensembles in the Western Hemisphere and the Indopacific with little known mixing. In this study, we detected, and confirmed, using multiple methods, the Indopacific kdr SNPs, both V1016G and S989P, in three recent collections from Osceola County, Florida. This represents a large expansion of the geographic range of the Indopacific kdr SNPs. We consider the implications of this finding on future insecticide resistance surveillance studies, including assessing the ability of our current screening tools to detect these SNPs. We find that the presence of the Indopacific SNPs requires the modification of existing resistance screening protocols and requires further work to understand the operational implications for mosquito control. Full article

Pulmonary hypertension (PH) is a prevalent and severe complication in dogs infected with Dirofilaria immitis. This study aimed to elucidate the progression of PH by analyzing radiographic parameters and the Right Pulmonary Artery Distensibility (RPAD) Index at three key time points: diagnosis (day 0), discharge (day 90), and six months post-discharge (day 270). Fifty-two heartworm-infected dogs were divided into two groups: non-hypertensive and hypertensive. Radiographic measurements, including Vertebral Heart Size (VHS), CrPA/R4 ratio, and CdPA/R9 ratio, along with the RPAD Index, were assessed on Days 0, 90, and 270. Results indicated that, in Group A, the RPAD Index improved significantly from 42% on Day 0 to 43.16% on Day 90, with no significant change by Day 270 (42%). In contrast, hypertensive dogs exhibited a persistently low RPAD Index, averaging 17% throughout this study (p < 0.001). Radiographic parameters in hypertensive dogs showed continuous elevation compared to non-hypertensive dogs, with significant increases in VHS, CrPA/R4, and CdPA/R9 ratios on day 270 compared to day 0 (p < 0.05). The results confirmed that PH persisted in dogs with D. immitis after adulticide treatment, highlighting the importance of regular radiographic monitoring for assessing and managing long-term outcomes in dogs with PH during and after adulticide treatment. Continuous surveillance is thus essential for the effective post-treatment management of PH in dogs. Full article

This study investigates the chemical composition of the essential oil obtained from the leaves of Bocageopsis multiflora (Mart.) R.E.Fr (Annonaceae), examining its effectiveness in combating both the larvae and adult forms of Aedes aegypti mosquitoes. Additionally, for a deeper understanding of the insecticidal activity, toxicity properties and molecular docking calculations were conducted using the main compounds of this essential oil. GC/MS analysis revealed the presence of 26 constituents, representing 95.2% of the essential oil, with the major components identified as the sesquiterpenes α-selinene, β-selinene, and β-elemene. Larvicidal assays demonstrated potent activity of this essential oil with significant LC₅₀ values of 40.8 and 39.4 μg/mL at 24 and 48 h, respectively. Adulticidal assessments highlighted strong efficacy with LC₅₀ of 12.5 µg/mL. Molecular docking analysis identified optimal interaction activities of α-selinene and β-selinene with key Aedes proteins. The in silico studies comparing synthetic insecticides with the major sesquiterpenes of the essential oil revealed that β-selinene exhibited a significantly higher binding affinity compared to the other two sesquiterpenes. Also, ADMET studies of the three main sesquiterpenes indicated acceptable drug-like properties. In these findings, safety evaluations showed low toxicity and skin sensitization for the main sesquiterpenes, contrasting with commercial synthetic insecticides. Therefore, in silico analyses suggest promising interactions with Aedes proteins, indicating its potential as an effective alternative to conventional insecticides These results show the larvicidal and adulticidal potential of the essential oil from Bocageopsis multiflora against Aedes aegypti, supported by its predominant constituents, α-selinene, β-selinene and β-elemene. Full article

This study explored the potential of Fe₃O₄, SnFe₂O₄, and CoFe₂O₄ nanoparticles as larvicidal and adulticidal agents against Aedes aegypti (A. aegypti) larvae and adults, which are vectors for various diseases. This research involved the synthesis of these nanoparticles using the coprecipitate method. The results indicate that CoFe₂O₄ nanoparticles are the most effective in both larvicidal and adulticidal activities, with complete mortality achieved after 96 h of exposure. SnFe₂O₄ nanoparticles also showed some larvicidal and adulticidal efficacy, although to a lesser extent than the CoFe₂O₄ nanoparticles. Fe₃O₄ nanoparticles exhibited minimal larvicidal and adulticidal effects at low concentrations but showed increased efficacy at higher concentrations. The study also revealed the superparamagnetic nature of these nanoparticles, making them potentially suitable for applications in aquatic environments, where A. aegypti larvae often thrive. Additionally, the nanoparticles induced observable damage to the gut structure of the mosquitoes and larvae, which could contribute to their mortality. Overall, this research suggests that CoFe₂O₄ nanoparticles, in particular, hold promise as environment-friendly and effective agents for controlling A. aegypti mosquitoes, which are responsible for the transmission of diseases such as dengue fever, Zika virus, and Chikungunya. Further studies and field trials are needed to validate their practical use in mosquito control programs. Full article

We performed a thorough systematic review of published literature to determine potential links between human health impacts and naled, a registered adult mosquito control product (adulticide), and its major degradate, dichlorvos (DDVP). A search query was performed on 8 September 2023, capturing all articles published up to that date on the Scopus and PubMed databases. Inclusion criteria were the presence of either pesticide and a measured or modeled human health outcome or risk. The search string resulted in 382 articles; however, 354 articles were excluded, resulting in only 28 articles that met the inclusion criteria. The studies that directly relate to aerosolized ultra-low volume (ULV) mosquito control applications did not report any associated deleterious human health outcomes. Results from the reviewed papers displayed no negative health effects or led to inconclusive results. No studies showed adverse health effects from aerial ULV applications for mosquito management. Our findings are congruent with the United States Environmental Protection Agency and Centers for Disease Control and Prevention recommendations that aerial applications of naled, following label parameters, do not pose an adverse risk exposure to humans, wildlife, and the environment. Full article

Mosquitoes are one of the main vectors of many important diseases and their degree of resistance to chemical insecticides has increased. Nowadays, it has become crucial to identify novel plant larvicides with an eco-friendly impact. The components of essential oils from Croton linearis Jacq. (EO-Cl), Lantana involucrata L. (EO-Li), Ocimum sanctum var. cubensis M. Gómez. (EO-Os), and Zanthoxylum pistaciifolium Griseb. (syn. Zanthoxylum flavum subsp. pistaciifolium (Griseb.) Reynel (EO-Zp) were determined using gas chromatography-mass spectrometry (GC–MS) analysis. Larvicidal and adulticidal bioassays against Aedes aegypti, Anopheles albitarsis and Culex quinquefasciatus, were performed according to the World Health Organization standard methods. A high diversity of compounds was identified in the four oils, with a total of 152 compounds (33–70 components). EO-Cl, EO-Li, and EO-Os were classified as active against both insect forms, larvae and adults. Lantana involucrata showed the best results, with LC₅₀ values from 33.8 to 41.7 mg/L. In most of the cases, it was not possible to associate the main compounds with the measured activity, supporting the hypothesis about probable synergistic interactions among major and minor compounds. The results indicate EO-Cl, EO-Os, and EO-Li as good eco-friendly insecticides with potential. Full article

Oligonychus coffeae (Acari: Tetranychidae), popularly known as red spider mite (RSM) is one of the major pests of commercial tea (Camellia sinensis (L.) O. Kuntze) plantation world over. Many attempts have been made in the past to control this devastating pest using a variety of microbial bioagents, however, area-wise field success is very limited. We carried out an in vitro study to explore the potential of rhizospheric Bacillus spp. (B. amyloliquefaciens BAC1, B. subtilis LB22, and B. velezensis AB22) against O. coffeae through adulticidal and ovicidal activity. The 100% adult and egg mortality was observed with bacterial suspension (1 × 10⁹ CFU/mL) by B. velezensis AB22, showing the lowest LC₅₀ values for both adults and eggs of O. coffeae, i.e., 0.28 × 10⁵ and 0.29 × 10⁵, respectively. The study also throws some insights into the underlying mechanism through electron microscopy study and identification of some putative pesticidal metabolites from all the species. The three Bacillus species were observed to have four commonly secreted putative bioactive secondary metabolites, brevianamide A, heptadecanoic acid, thiolutin, and versimide responsible for their bio-efficacy against O. coffeae. The outcome of our study provides a strong possibility of introducing Bacillus spp. as a biological miticide and developing synthetic metabolites mimicking the mechanistic pathway involved in microbial bioefficacy. Full article

Essential oils are natural plant products that are very interesting, as they are important sources of biologically active compounds. They comprise eco-friendly alternatives to mosquito vector management, particularly essential oil nanoemulsion. Therefore, the aim of this study is to evaluate the effectiveness of 16 selected essential oils (1500 ppm) in controlling mosquitoes by investigating their larvicidal effects against the larvae and adults of the West Nile virus vector Culex pipiens L. (Diptera: Culicidae); the best oils were turned into nanoemulsions and evaluated under laboratory and field conditions. The results show that honeysuckle (Lonicera caprifolium) and patchouli (Pogostemon cablin) essential oils were more effective in killing larvae than the other oils (100% mortality) at 24 h post-treatment. The nanoemulsions of honeysuckle (LC₅₀ = 88.30 ppm) and patchouli (LC₅₀ = 93.05 ppm) showed significantly higher larvicidal activity compared with bulk honeysuckle (LC₅₀ = 247.72 ppm) and patchouli (LC₅₀ = 276.29 ppm) oils. L. caprifolium and P. cablin (100% mortality), followed by Narcissus tazetta (97.78%), Rosmarinus officinalis (95.56%), and Lavandula angustifolia (95.55%), were highly effective oils in killing female mosquitoes, and their relative efficacy at LT₅₀ was 5.5, 5.3, 5.8, 4.1, and 3.2 times greater, respectively, than Aloe vera. The results of the field study show that the honeysuckle and patchouli oils and their nanoemulsions reduced densities to 89.4, 86.5, 98.6, and 97.0% at 24 h post-treatment, respectively, with persistence for eight days post-treatment in pools. Nano-honeysuckle (100% mortality) was more effective than honeysuckle oils (98.0%). Our results show that honeysuckle and patchouli oils exhibited promising larvicidal and adulticidal activity of C. pipiens. Full article