Search Results (159)

Background/Objectives: Differentiating adductor spasmodic dysphonia (AdSD) from muscle tension dysphonia (MTD) is challenging because their auditory–perceptual features often overlap. We examined whether high-speed videolaryngoscopy (HSV) combined with two-dimensional digital kymography (2D-DKG) and line DKG yields qualitative signs and quantitative metrics that distinguish AdSD from MTD. Methods: We analyzed vocal fold vibration in eight patients with AdSD, eight with primary MTD, and eleven vocally healthy controls using a multifunctional system integrating HSV, 2D-DKG, and line DKG. Qualitative features (glottal closure, mucosal wave, phase and amplitude symmetry, oscillatory breaks, and supraglottic hyperfunction) and quantitative indices (closed quotient [CQ], speed quotient [SQ], phase symmetry index [PSI], amplitude symmetry index [ASI]) were assessed. Group differences were tested with one-way ANOVA and Scheffé post hoc comparisons. Results: Oscillatory breaks were observed in 75% of AdSD cases and in 0% of MTD and controls, whereas supraglottic hyperfunction occurred in 100% of MTD and in 0% of AdSD and controls. Quantitatively, CQ, SQ, PSI, and ASI differed between dysphonic groups and controls (p < 0.05), but no quantitative index discriminated against AdSD from MTD. Conclusions: HSV with 2D-/line-DKG provides complementary, objective information on vibratory patterns and supraglottic behavior, supporting targeted qualitative assessment in the clinical differentiation between AdSD and MTD, and highlighting the need for its incorporation into clinical practice as a procedure to assist in the complex diagnostic distinction between these conditions. Full article

Adductor-related groin pain (AGP) is a prevalent and frequently recurrent chronic injury among soccer players. This study investigated the impact of AGP history on kicking kinematics, kinetics, and patient-reported outcomes in regional-league soccer players using one-dimensional statistical parametric mapping (1D-SPM). Twenty male athletes were allocated to a group with prior AGP (GP group: n = 8) or without AGP (non-GP group, n = 12), and evaluated during maximal instep and inside-foot kicks using three-dimensional motion analysis and the Copenhagen Hip and Groin Outcome Score (HAGOS). The GP group reported significantly lower HAGOS for pain and quality of life. The 1D-SPM analysis revealed that the GP group employed a compensatory kinetic chain strategy, characterized by impaired trunk–pelvis rotation, increased reliance on the stance leg (SL) for stability, and altered kicking leg (KL) mechanics with reduced hip flexion power. These findings reveal that the underlying deficit in AGP is not isolated muscle weakness but a ‘lack of adaptability in motor control’, resulting in inefficient load distribution and contributing to the high recurrence rates in the adductors and SL. Rehabilitation should adopt a kinetic chain-oriented approach that also addresses stance limb function to mitigate recurrence and optimize performance. Full article

Background/Objectives: Sarcopenia and muscle composition have emerged as significant indicators in the fields of musculoskeletal and metabolic research. The objective of this study was to develop and validate a fully automated, deep learning-based method for segmenting thigh muscles into three functional groups (extensor, flexor, and adductor) using non-contrast computed tomography (CT) images and to quantitatively evaluate the thigh muscles. Methods: In order to ascertain the most efficacious architecture for automated thigh muscle segmentation, three deep learning models (Dense U-Net, MANet, and SegFormer) were implemented and subsequently compared. Each model was trained using 136 manually labeled non-contrast thigh CT scans and externally validated with 40 scans from another institution. The performance of the segmentation was evaluated using the Dice similarity coefficient (DSC), sensitivity, specificity, and accuracy. Quantitative indices, including total muscle volume, lean muscle volume, and intra-/intermuscular fat volumes, were automatically calculated and compared with manual measurements. Results: All three models exhibited high segmentation accuracy, with the mean DSC exceeding 96%. The MANet model demonstrated optimal performance in internal validation, while the SegFormer model exhibited superior volumetric agreement in external validation, as indicated by an intraclass correlation coefficient (ICC) of at least 0.995 and a p-value less than 0.01. Conclusions: A CT-based deep learning framework enables accurate and reproducible segmentation of functional thigh muscle groups. A comparative evaluation of convolutional attention- and transformer-based architectures supports the feasibility of CT-based quantitative muscle assessment for sarcopenia and musculoskeletal research. Full article

New Zealand abalone (Haliotis iris) holds ecological, economic, and cultural value, with wild stocks supporting fisheries and an emerging aquaculture industry. Wild-caught adult abalone are often used as broodstock, but captivity can affect spawning and offspring quality. This study is the first to profile wild and farmed H. iris broodstock using histology, proximate composition, microbiome, and metabolomics analyses. Histology showed higher gonadal abnormalities in farmed abalone, while wild abalone exhibited increased ciliates in their gills, indicating richer marine–microorganism interactions. Microbiome analyses revealed a higher microbial richness and diversity in the buccal cavity of wild abalone. The core microbiota phyla across both groups included Proteobacteria, Bacteroidota, Campylobacterota, Fusobacteria, and Firmicutes. Proximate analyses showed higher muscle protein in farmed abalone, while gonadal tissue partitioned by sex showed higher fat in females and higher protein in males. Metabolomics revealed altered amino acid metabolism in the adductor muscle, carboxylic acid metabolism in the gonad, and fatty acid metabolism in the foot. This investigation expands our understanding of the physiological and microbial differences between wild and farmed abalone, showing altered gonad and muscle conditions from prolonged captivity and highlighting the need for greater microbial diversity in cultured stocks. Full article

The lion’s paw scallop (Nodipecten subnodosus) is a commercially valuable pectinid whose postharvest quality strongly depends on storage and handling conditions. This study investigated the combined effects of seasonality, postmortem time, and storage temperature on energy metabolism in the adductor muscle, focusing on metabolites associated with rigor mortis and freshness. Adult scallops (~10 cm shell height) were harvested in four seasons (spring, summer, autumn, winter), transported under commercial conditions for approximately 2 h, and stored at 0, 5, and 10 °C for 48 h. Muscle samples were collected every 8 h and analyzed for ATP, ADP, AMP, glycogen, arginine phosphate (Arg-P), and free arginine using HPLC and enzymatic assays. In addition, the adenylate energy charge (AEC) was determined in freshly harvested and post-transport specimens. Initial ATP concentrations ranged from 4.2 to 6.5 µmol/g, with higher levels in winter, while Arg-P varied from 3.1 to 4.8 µmol/g. Seasonality significantly influenced all metabolites except arginine, and transport markedly reduced ATP and AEC, particularly in spring and autumn. Storage at 0 °C resulted in rapid ATP depletion (<1.0 µmol/g within 12 h) and AMP accumulation (>3.0 µmol/g), indicating accelerated energy collapse. In contrast, scallops stored at 5 and 10 °C maintained ATP levels above 2.5 µmol/g for up to 24 h, delaying rigor mortis, reducing postmortem contraction, and preserving muscle texture and appearance. Overall, these findings demonstrate that moderate refrigeration represents a physiologically suitable and technologically advantageous strategy to optimize scallop postharvest handling, extend shelf life, and enhance product quality for the fresh seafood market. Full article

Background and Objectives: Horse riding simulators (HRS) provide rhythmic, repetitive, and multidirectional movements analogous to horseback riding, which may facilitate postural control, balance, and functional abilities in children with cerebral palsy (CP). This study aimed to investigate the effects of the HRS application on the muscle tone of the lower extremity, gross motor function, trunk postural control, balance, gait functions, and functional independence in children with CP. Materials and Methods: A quasi-experimental study included 30 children with cerebral palsy (17 hemiparetic, 13 diparetic; mean age, 9.3 ± 3.2 years). All participants received Neurodevelopmental Therapy (NDT) for eight weeks, followed by eight weeks of HRS plus NDT, in a sequential design. Outcomes included the Modified Ashworth Scale (MAS), Myoton^®PRO, Gross Motor Function Measures (GMFM)-88, Pedalo^® Sensamove Balance Test (Pedalo^® SBT), Pediatric Balance Scale (PBS), Trunk Impairment Scale (TIS), gait analysis parameters, and Functional Independence Measure (WeeFIM). Assessments were made at baseline, the 8th, and the 16th week. Results: At week 16, after incorporating HRS, all MAS parameters demonstrated greater improvements compared to those achieved during the first eight weeks of NDT alone (ES: 0.728–0.931, p < 0.05). Myoton^®PRO measurements showed a significant reduction in gastrocnemius stiffness (ES = 0.672, p < 0.05) in hemiparetic children and decreases in hip adductor (ES: 0.649, p < 0.05) and gastrocnemius-soleus (ES: 0.766–0.865, p < 0.05) stiffness from week 8 to 16 in diparetic children following HRS intervention. Total scores on the GMFM-88, WeeFIM, TIS, and PBS improved significantly, with large effect sizes observed both from baseline to week 16 and from week 8 to 16 (ES: 0.771–0.886, p < 0.05). Additionally, Pedalo^® SBT scores increased following HRS intervention from baseline to week 16 (ES = 0.599–0.602, p < 0.05). Conclusions: HRS integrated with conventional NDT may improve muscle tone, motor function, balance, gait, and functional independence in children with cerebral palsy, representing a valuable adjunct to standard rehabilitation. These findings provide the first evidence that simulator-assisted interventions may benefit daily activities in children with cerebral palsy. Full article

Background: Multimodal analgesia, combining adductor canal block (ACB) and local infiltration analgesia (LIA), is commonly used for pain control after total knee arthroplasty (TKA). However, ACB alone may not fully cover the anteromedial knee, a region extensively disrupted by TKA. Recent studies suggest that blocking branches of the anterior femoral cutaneous nerve (AFCN) could enhance analgesia, but targeted AFCN blocks are technically challenging. We evaluated supra-sartorial subcutaneous infiltration (SSSI) at the femoral triangle apex as a simpler alternative to AFCN blocks. Methods: We retrospectively reviewed 19 patients undergoing TKA with a standardized multimodal analgesic protocol, including intraoperative LIA limited to posterior capsule (PC-LIA), postoperative SSSI, and delayed intermittent ACB via catheter. SSSI involved infiltrating 20 mL of 0.3% ropivacaine into the subcutaneous plane above the sartorius muscle at the level of femoral triangle apex. Pain was assessed using Numerical Rating Scale (NRS) scores at rest and during movement at 9:00 PM on postoperative day 0 (POD 0) and 9:00 AM on POD 1, with scheduled ACB doses administered at the time of NRS pain score assessments. Rescue ACB boluses were given for intolerable pain before the first scheduled dose. Results: Eleven patients (58%) required no rescue analgesia before the first scheduled ACB, maintaining NRS scores ≤ 4 at rest and with movement for a minimum of 575–785 min post-spinal anesthesia. Eight patients needed rescue ACB, with variable pain relief. Conclusions: SSSI, when combined with PC-LIA, provided clinically meaningful analgesia in 58% of our patient cohort following TKA, though the variability observed suggests limited consistency. As a practical alternative to targeted AFCN blocks, SSSI could potentially complement ACB in multimodal pain management, but its efficacy remains uncertain due to the retrospective, non-controlled study design without a comparator group. Further investigation through prospective randomized controlled trials is warranted to validate these preliminary findings. Full article

Peripheral artery disease is a common manifestation of atherosclerosis, characterized by insufficient tissue perfusion and chronic ischemia. Arteriogenesis and angiogenesis are essential endogenous mechanisms to restore blood flow and limit ischemic injury. The metalloprotease ADAMTS13, known for cleaving ultra-large von Willebrand factor, has been implicated in thrombotic and inflammatory regulation. However, its role in ischemic vascular remodeling remains unclear. Using a murine hind limb ischemia model, we investigated the effect of ADAMTS13 deficiency on arteriogenesis and angiogenesis by comparing male ADAMTS13^−/− and wild-type control mice. Perfusion recovery, vascular cell proliferation, immune cell infiltration, and thrombotic activity were evaluated using laser Doppler measurements, immunohistochemical analysis of adductor and gastrocnemius muscle tissues, and in vivo microscopy. ADAMTS13 deficiency did not impair perfusion recovery, collateral artery growth, or capillarization. While platelet adhesion was slightly increased in ADAMTS13^−/− mice, no thrombotic occlusions were observed. Inflammatory responses, including macrophage and neutrophil infiltration as well as macrophage polarization, were largely unaffected. Despite previous in vitro evidence indicating an angiogenic role for ADAMTS13, its absence did not compromise angiogenesis in vivo. Our findings suggest that ADAMTS13 does not play a critical role in ischemia-related angiogenesis and arteriogenesis under sterile conditions and may be relevant only in contexts involving acute and sufficiently strong thromboinflammatory stimuli. Full article

Background: At present, the rotational function of the hip adductor muscle group remains unclear. This study aimed to clarify the rotational function and stabilizing role of the pectineus, adductor longus, and adductor brevis (adductor muscle group) based on anatomical findings and T₂ values (ms) obtained from magnetic resonance imaging (MRI). T₂ values are prolonged in tissues with higher water content, and in skeletal muscle, it has been demonstrated that T₂ values increase in proportion to exercise intensity. Methods: Using fixed specimens (n = 6, aged 61–96 years), we observed the three-dimensional arrangement of muscles in the neutral position of the hip joint and observed the extension or shortening of muscles associated with passive maximum internal and external rotation of the hip joint. In addition, we evaluated the activity of the adductor muscle group by T₂ values (ms) from MRI pre- and post-internal rotation (forward step with the left leg) and pre- and post-external rotation (backward step with the left leg) movements of the right hip joint in a standing position (n = 8, healthy adult subjects, mean age 29.1 ± 5.3 years). Results: Regarding functional anatomy, the arrangement of the gluteus minimus and adductor muscle groups was almost parallel across the femoral neck. In the evaluation of adductor muscle group activity using MRI, the percent change in T₂ values (%) of the pectineus was 6.38 ± 1.35 pre- and post-internal rotation and 1.35 ± 0.71 pre- and post-external rotation, whereas that of the adductor longus and brevis was 4.84 ± 1.31 pre- and post-internal rotation and 1.31 ± 0.68 pre- and post-external rotation. The percent change in T₂ values pre- and post-internal rotation exercise was significantly greater than that pre- and post-external rotation exercise in the pectineus, adductor longus, and brevis muscles (p < 0.05). Conclusions: The adductor muscle groups are suggested to contribute to joint stability in the coronal plane and provide joint internal rotation in the standing position. Full article

Structural analysis of glycosphingolipids provides novel insights into organismal classification and reveals conserved functional roles that transcend taxonomic boundaries. To elucidate the structural characteristics of acidic glycosphingolipids (AGLs) in the adductor muscle of the Japanese giant scallop (Patinopecten yessoensis), AGLs were isolated and purified by column chromatography using anion exchange resin and silica gel. Structural characterization was performed using mass spectrometry, proton nuclear magnetic resonance spectroscopy, and immunological techniques. The sugar chain structure was identified as GlcA4Meβ1-4(GalNAc3Meα1-3)Fucα1-4GlcNAcβ1-2Manα1-3Manβ1-4Glcβ1-Cer, consistent with the mollu-series core reported for mollusks. In addition to uronic acid, the structure was distinguished by internal fucose and methylated sugars, features commonly found in bivalves. The presence of xylose in the sugar chains of AGLs was also suggested. In contrast, the ceramide moiety was composed primarily of fatty acids C16:0 and C18:0 and the long-chain base d16:1. This chemical structure provides valuable insights into the biological classification of P. yessoensis and the mollu-series glycolipids containing fucose and methylated sugars, which may serve as bioactive components shared across species in the phylum Mollusca and class Bivalvia. Full article

Background: Inline skating has rapidly grown in popularity. Early research primarily focused on injury patterns and protective measures. However, its biomechanical similarity to other skating modalities enables the synthesis of existing evidence, emphasizing key physical attributes essential for performance, namely, balance and the strength of the hip adductor and abductor muscles. The interaction between these muscle groups in relation to balance has not yet been examined in inline skaters. This study aimed to investigate the relationship between single-leg static balance and the isometric strength of the hip adductors and abductors, including their strength ratio. Methods: A total of 191 amateur inline skaters (aged 18 to 59 years) were evaluated. Balance was assessed through center of pressure displacement using the Footscan^® 9 platform, and the maximal isometric strength of the hip adductors and abductors was measured using a handheld dynamometer. A linear regression on the center of pressure (CoP) displacement was performed. Results: Age, sex, and skating frequency were the most influential predictors (p < 0.001), although strength variables also significantly predicted the CoP (p <0.05). Conclusions: Superior balance performance was observed in younger individuals, women, and those practicing five or more days a week. Furthermore, single-leg static balance was associated with an equilibrium between adductor/abductor strength, particularly when a low ratio was accompanied by high levels of hip adductor strength. Full article

Background and Objectives: Chronic ankle instability (CAI), a prevalent injury among female volleyball players, can negatively affect functional performance and increase the risk of further injury. The aim of this study was to compare functional movement quality, dynamic balance, vertical jumping performance, hip muscle strength, and risk of injury between adolescent female volleyball players with unilateral CAI and those without CAI. Materials and Methods: This cross-sectional study included 46 adolescent female volleyball players, divided into CAI (n = 23) and control (n = 23) groups based on predefined criteria. Functional movement quality was assessed using the Functional Movement Screen (FMS), and dynamic balance was evaluated with the Y-Balance Test (YBT). Maximal isometric strength of the hip muscles (flexors, extensors, abductors, adductors, and internal and external rotators) was measured using hand-held dynamometry, and vertical jumping performance was assessed using countermovement jump tests. Injury risk was classified based on established cut-off values for the FMS-composite and YBT-anterior reach asymmetry scores. Results: The CAI group demonstrated significantly lower FMS-composite scores (p = 0.007), reduced anterior reach on the YBT (p = 0.004), and decreased strength in the hip flexors (p = 0.007) and hip adductors (p = 0.044), supported by moderate effect sizes. No significant group differences were observed in the other YBT directions, vertical jump tests, or the other hip muscles (p > 0.05). A greater proportion of athletes in the CAI group were classified as high risk for injury based on both FMS-composite (p = 0.022) and YBT-anterior reach asymmetry (p = 0.001) cut-off values, supported by moderate and relatively strong effect sizes, respectively. Conclusions: Adolescent female volleyball players with unilateral CAI showed impaired movement quality, balance deficits, hip muscle weakness, and increased injury risk. These results highlight the importance of targeted interventions and broader investigations into CAI in adolescent athletes. Full article

Background: Body composition assessment is important in professional football as a measure of overall adaptation of the athlete to the training demand and calorie intake. However, it is operator-dependent, relying on subject positioning and the focus angle of the X-rays. In addition, the usual appendicular lean mass index (ALMI) does not include the pelvitrochanteric muscles, which are often implicated in athlete injuries. Methods: Three independent operators compared the reproducibility of dual-energy X-ray absorptiometry (DEXA) including pelvi-trochanteric muscle mass in twenty professional football players, using the standard and a new method. Results: Mean age, weight, and height of the footballers were 25.9 ± 4.9 years, 79.4 ± 9.4 kg, and 1.83 ± 0.09 m. Using the standard method, the ALMI was 9.28 ± 0.62, 9.20 ± 0.65, and 9.13 ± 0.64 kg/m² for the first, second and third operator, respectively. When including the pelvi-trochanteric muscles, the ALMI values were 11.90 ± 0.66, 11.84 ± 0.63, and 11.83 ± 0.65 kg/m² for the three operators. The difference between the two methods was significant (p < 0.001). The mean inter-operator difference was similar regardless of the method used (0.099 ± 0.06 kg/m²). The intraclass correlation coefficients (ICC) (A,1) were 0.949 [0.881; 0.979] for the standard method and 0.977 [0.951; 0.990] for the new method. The ICC (C,1) was 0.960 [0.918; 0.983] for the standard method and 0.979 [0.957; 0.991] for the new method. Conclusions: Thus, both new and conventional methods showed excellent reproducibility. However, reproducibility and inter-operator variability were better with the adjustment of the new scan lines. Moreover, the inclusion of a larger gluteal and adductors muscle mass was easy to achieve, providing additional information that could potentially be useful for early diagnosis and/or prevention of future muscular injuries in elite athletes. Full article

Water treadmill (WT) exercise has become a popular tool for equine training and rehabilitation. However, few studies describe the long-term effects of WT exercise in low water on muscle development (MD). This study’s objectives were to compare MD changes over a 40-week period in sport horses that regularly used WT in low water within training (Group WTH, n = 55) and a control group that did not (control, n = 28). Subjective MD assessments were undertaken using an adaptation of a previously published method at weeks 0, 20 and 40. For Group WTH, MD significantly increased in the neck, pelvis and hindlimb adductor and abductors between weeks 0 and 20. Neck, thoracic, thoracic trapezius, lumbar, pelvis, quadriceps, hindlimb adductor and abductor, and hamstring musculature increased between weeks 0 and 40. Thoracic musculature only increased between weeks 20 and 40 (p ≤ 0.001 for all). In the control horses, MD did not significantly change between weeks. Regular WT exercise appears to increase MD, particularly for musculature used to create movement patterns seen on the WT. WTs may be appropriate for use under veterinary guidance as part of a directed rehabilitation/training programme to increase core and hindlimb MD. Full article

Background: This study includes an investigation of lower-limb muscle strength and physical activity among children in Japan and China, with the aim of promoting children’s health. Methods: A total of 564 children (300 boys, 264 girls) aged 9–12 years from public primary schools in Tokyo, Japan, and Jiangxi Province, China, were included. Height, body weight (BW), hip adductor and abductor strength, and toe grip strength were measured. The side-step test (SST) and timed-up-and-go test (TUGT) were performed. Exercise habits were assessed via a questionnaire. Results: Japanese students produced significantly higher values than Chinese students in SST (23.3–37.1%) and TUGT (6.6–8.0%), except among 11-year-old girls. Japanese boys aged 10–11 and girls aged 10–12 had significantly greater hip adductor strength/BW and toe grip strength/BW. Japanese students also showed significantly higher hip abductor strength/BW at all ages. Additionally, the proportion of children engaging in vigorous-intensity physical activity (VPA) was significantly higher in Japanese boys aged 10–12 and girls aged 9–11 than in their Chinese counterparts. Conclusions: Chinese students showed diminished lower-limb strength and agility compared to Japanese students. These findings highlight the importance of promoting outdoor play, particularly VPA, to improve children’s physical fitness and health, especially in China. Full article