Search Results (80,531)

Corn is a critically important agricultural crop, with different parts consumed at specific growth stages—notably, fresh corn and corn grain. The field residue behaviors of clothianidin and phoxim in fresh corn and corn grain were investigated by using a QuEChERS technique combined with UPLC-MS/MS, and the dietary exposure risks of the insecticides were evaluated. Field trials were conducted under the recommended application conditions (2250 g active ingredient per hectare of 2% clothianidin and phoxim granules, applied once) at 12 agricultural regions across China under various climatic conditions to study the residue levels of pesticides. The samples were collected at harvest of fresh corn and corn grain, separately. The results indicated that the residues of the phoxim remained stable in all samples stored at −20 °C for 6 months (180 days). At harvest, the concentrations of clothianidin in fresh corn ranged from <0.01 to 0.027 mg/kg (below the MRL of 0.1mg/kg established by Japan); the concentrations of phoxim in fresh corn were all below 0.01 mg/kg (below the Chinese MRL of 0.1mg/kg). In corn grain, clothianidin residues ranged from <0.01 to 0.018 mg/kg (below the Chinese MRL of 0.02mg/kg), while phoxim residues were below 0.01mg/kg (below the Chinese MRL of 0.1mg/kg). Dietary risk assessment based on Chinese pesticide registration status and the per capita dietary structure of Chinese residents showed that the national estimated daily intake (NEDI) of clothianidin for the general population was 0.49 mg, accounting for 7.8% of the adult acceptable daily intake (ADI), while NEDI of phoxim for the general population was 0.048 mg, accounting for 19.2% of adult ADI. The results suggest that the associated health risks are acceptable for the general population. On the basis of these findings, it is recommended to establish an MRL for clothianidin in fresh corn in China at 0.1 mg/kg. This study provided basic data on the use and safety of clothianidin and phoxim in fresh corn and corn grain to help the Chinese government formulate a maximum residue level for clothianidin in fresh corn. Full article

Firefighters are exposed to complex combustion products and to contaminants carried on personal protective equipment (PPE). Occupational exposure as a firefighter is classified as carcinogenic. This review summarizes the current evidence on exposure environments, routes of uptake, contamination and secondary exposure from PPE, and the effectiveness and limits of decontamination approaches. Across incident types, smoke composition varies with the fuels and combustion conditions, but fine and ultrafine particles and semi-volatile organic chemicals are common. Biomonitoring confirms uptake after incidents. Self-contained breathing apparatus reduces inhalation exposure during active suppression, yet exposures persist through dermal absorption at ensemble interfaces and post-incident tasks. Protective ensembles can retain soot-bound polycyclic aromatic hydrocarbons, additive chemicals, and metals; volatiles and particles resuspension in vehicles and stations can extend exposure. Studies show that on-scene preliminary exposure reduction and laundering can lower contaminant burdens on PPE; however, removal remains incomplete and decreases when cleaning is delayed or when gear is aged. Emerging evidence raises additional concern for per- and polyfluoroalkyl substances from foams and coating materials, with limited data on exposure metrics and removability. The field lacks standardized, realistic contamination platforms and a dose-based definition of clean PPE. Integrated intervention studies linking exposure, secondary exposure pathways, biomarkers, and decontamination methods are needed to set performance-based targets and evaluate emerging hazards. Full article

Palm oil is a major agricultural commodity and an important economic driver in Asia. However, the sustainability and productivity of this crop are constantly threatened by a range of pathogenic fungi, especially Ganoderma boninense. Therefore, this study aimed to develop an eco-friendly hexaconazole-loaded nanoemulsion (Hexa-NE) for effective and targeted fungicide delivery while reducing environmental and health impacts. The optimized Hexa-NE formulation was evaluated for particle size, polydispersity index (PDI), zeta potential, pH, viscosity, and morphology using Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). Fungicide release, stability, and antifungal activity were conducted to assess the overall efficacy and performance of the formulation. The Hexa-NE exhibited particle size of 105.8 nm, a PDI of 0.358, a zeta potential of −53.53 mV. The formulation remained stable over three months of storage. It also demonstrated favourable physicochemical properties including low viscosity (30.24 mPa·s), low surface tension (23.87 mN/m), and suitable pH (6.14) for foliar application. TEM and SEM analyses confirmed spherical droplets and revealed significant hyphal damage to G. boninense. The antifungal test showed a higher inhibition of 97.1% at 0.1 µM of Hexa-NE as compared to hexaconazole solution which only 40% at the same concentration. Release studies exhibited a sustained release of hexaconazole, which may prolonged fungicidal activity. In conclusion, Hexa-NE showed promising laboratory-scale antifungal performance against G. boninense. These findings support its potential for further investigation as a nanoformulated fungicide for future greenhouse and field evaluations. Full article

In this study, the feasibility of electrospraying as an alternative processing technique for the preparation of composite solid rocket propellants (SRPs) was investigated. The main objective was to improve microstructural homogeneity and interfacial contact between the oxidizer, energetic additive, and metallic fuel without altering the chemical composition of the formulation. Additionally, porous electrosprayed SRP formulations were prepared to examine the influence of controlled porosity on thermal decomposition behavior. The prepared materials were characterized using scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (SEM/EDS) to assess microstructural features and component distribution. Thermal decomposition behavior and kinetic parameters were evaluated using simultaneous DSC/TG analysis conducted at multiple heating rates. Safety-related properties were assessed through friction sensitivity testing, while post-decomposition solid residues were analyzed using SEM/EDS and X-ray diffraction. The results show that electrospraying improves structural homogeneity, reduces solid residue formation after thermal decomposition, and decreases apparent activation energy, while maintaining unchanged friction sensitivity. These findings demonstrate the potential of electrospraying as a physical processing route for tailoring the microstructure and thermal behavior of composite solid rocket propellants. Full article

Oxidative stress has emerged as a key factor regulating female fertility, reproductive aging, and the development of various gynecologic and pregnancy-associated diseases. While physiological concentrations of reactive oxygen species play a fundamental role in many aspects of normal reproduction such as folliculogenesis, oocyte maturation, implantation, and placental development, abnormal or chronic oxidative stress impairs redox homeostasis and promotes mitochondrial dysfunction, inflammation, DNA damage, and cellular senescence. Recent research interest has shifted toward next-generation dietary antioxidants, including bioactive polyphenols, carotenoids, micronutrients, and nutraceutical combinations with improved bioavailability and molecular targets. These compounds go beyond classical free-radical scavenging activity and modulate a network of redox-sensitive signaling pathways involved in autophagy, apoptosis, endocrine regulation, and immunological balance. In this review, we integrate current mechanistic advances into a cohesive framework that illustrates the regulation of key cellular processes affecting female reproductive physiology by next-generation dietary antioxidants. We also critically evaluate experimental, translational, and clinical data supporting their role in promoting reproductive outcomes, including oocyte quality, ovarian reserve, pregnancy success, and mitigation of age-related reproductive decline. We highlight their potential in the therapeutic intervention of oxidative stress-related conditions such as infertility, polycystic ovary syndrome, endometriosis, early ovarian insufficiency, and menopause-associated disorders. Finally, we discuss the current challenges associated with dosage optimization, bioavailability, long-term safety, and interindividual variability. We conclude by highlighting next-generation dietary antioxidants as a promising, widely available, and non-invasive approach to improve women’s reproductive health and promote fertility throughout their lifespan. Full article

We examined gender and racial parity in agricultural education enrollment and engagement among 230,228 students representing 1245 secondary-level agricultural education programs in nine U.S. states. Guided by the Representative Bureaucracy Theory, we evaluated agricultural education demographic enrollment and compared it to the demographics of the school that each program served to assess gender and racial representation differences. We also analyzed engagement patterns in Supervised Agricultural Experiences (SAE) and FFA activities by comparing the agricultural education enrollment to program engagement data. The results indicated that minority students were enrolled in school-based agricultural education (SBAE) programs at rates slightly below their representation in the school population. However, engagement data suggested that females were enrolled in agricultural education at expected rates but participated in SAE and FFA at higher-than-expected rates. These findings highlighted modest discrepancies in demographic representation and engagement, though the differences were generally minor and reflect progress toward inclusivity. The study concludes that, while increasing diversity and parity remain goals, agricultural education programs have made measurable strides in engaging broader student audiences. Recommendations include continued efforts to recruit and support underrepresented groups in SBAE, with a focus on both enrollment and meaningful participation. Full article

This paper explores the feasibility of employing small language models (SLMs) on edge devices powered by batteries in environments with limited/no internet connectivity. SLMs in fact offer significant advantages in such scenarios due to their lower resource requirements with respect to large language models. The use case in this study is maritime navigation—in particular, the documentation on Sailing Directions (Enroutd) of the World Port Index (WPI) provided by the National Geospatial-Intelligence Agency (NGA), which provides information that cannot be shown graphically on nautical charts and is not readily available elsewhere. In this environment, response immediacy is not critical, as users have sufficient time to query information while navigating and planning activities, making edge devices ideal for running these models. On the contrary, the response quality is fundamental. For this reason, given the constrained knowledge of SLMs in maritime contexts, we investigate the use of the retrieval-augmented generation (RAG) methodology, integrating external information from sailing directions. A comparative analysis is presented to evaluate the performance of various state-of-the-art SLMs, focusing on response quality, the effectiveness of the RAG component, and inference times. Full article

Chronic alcohol consumption exacerbates kidney injury, particularly in individuals with hepatitis B virus (HBV) infection. This study investigated the protective effects of boric acid supplementation against alcohol-induced renal damage in HBV transgenic mice. HBV transgenic mice were divided into four groups: control (C), boric acid (B), alcohol (A), and alcohol + boric acid (A + B). Renal injury was evaluated using H&E, PAS, TUNEL, and desmin staining. The expression of caspase-3, cytochrome c, and APAF-1 was analyzed by qRT-PCR. Biochemical analyses included BUN, creatinine, oxidative stress markers (ROS, MDA, TOS, OSI), total antioxidant status, and antioxidant enzyme activities (SOD, CAT, GPx). Histopathological findings showed activated parietal epithelial cells in all groups, indicating renal injury. Alcohol significantly increased tubular damage, podocyte desmin expression, apoptosis, cytochrome c and APAF-1 mRNA levels, and oxidative stress markers, while reducing antioxidant enzyme activities and BUN levels compared with controls. Boric acid supplementation significantly mitigated alcohol-induced tubular injury, apoptosis, oxidative stress, and serum creatinine levels, and improved BUN values. Boric acid treatment alone also alleviated glomerular and tubular injury and reduced tubular apoptosis compared with HBV control mice. Overall, boric acid exerts renoprotective effects in HBV-transgenic mice subjected to chronic alcohol exposure by inhibiting oxidative stress, apoptosis, and podocyte injury. Full article

Foot diseases represent a major cause of impaired welfare and reduced productivity in dairy ruminants. This pilot study aimed to evaluate the clinical progression of foot lesions in dairy cattle by combining clinical examination with hematochemical analyses and infrared thermography of foot (central zone, R1; interdigital area, R2; lateral claw, R3; medial claw, R4), in order to assess the effectiveness of therapeutic interventions and the evolution of inflammation over time. The study involved dairy cows affected by different foot disorders that underwent functional and therapeutic trimming. Ten lactating cows with hoof diseases (DG) were tested and 5 healthy animals (CG) were selected to serve as a control group. The animals were evaluated and sampled (blood sampling and acquisition of thermographic foot images) at three different time points: clinical diagnosis of the foot pathology (T0), 24 h (T1) and 10 days (T2) after treatment. Higher neutrophils, lymphocytes, platelets, α1-(p < 0.05) and α2-fraction values were found in diseased subjects compared to healthy ones at T0 (p < 0.05). Thermography highlighted significantly higher foot thermal values in animals with lesions than in healthy animals (p < 0.01), especially at T0. Animals affected by hoof diseases showed lower foot temperature values at T1 compared to T0 and T2 (p < 0.001). Though the small sample size and the short observation period limited the possibility of extending the results to the entire cow population, the infrared thermography proved to be a valuable non-invasive tool for detecting active lesions and monitoring healing, with higher temperatures corresponding to inflamed areas and a progressive normalization after treatment. The combined evaluation of clinical signs, systemic inflammatory markers and thermographic patterns provided an integrated perspective on the progression of foot diseases. Full article

Virgin olive oil (VOO) quality is strongly influenced by olive cultivar and fruit maturity stage, yet their combined effects remain insufficiently characterized in many traditional olive-growing regions. This study evaluated the physicochemical parameters, phenolic compounds content, antioxidant activity, fatty acid profile, volatile compounds, and sensory attributes of VOOs obtained from five autochthonous cultivars of Extremadura (Spain)—‘Corniche’, ‘Manzanilla Cacereña’, ‘Morisca’, ‘Pico Limón’, and ‘Verdial de Badajoz’—harvested at three ripening stages (Green, Verging-on-ripe, and Ripe). Early harvest oils exhibited significantly higher total phenolic content (up to 478 mg/kg expressed by caffeic acid equivalent, CAE), oxidative stability (up to 188 h), intense green-fruity notes dominated by (E)-2-hexenal and (Z)-3-hexenal, and stronger bitterness and pungency. As ripening progressed, phenolic compounds and LOX-derived C6 volatiles markedly decreased, while oil yield, linoleic acid, saturated aldehydes, and oxidation markers increased in most cultivars. Cultivar-specific responses were evident: ‘Corniche’ and ‘Manzanilla Cacereña’ maintained higher oleic acid and stability, whereas ‘Morisca’ and ‘Pico Limón’ were more prone to phenolic compound loss and sensory deterioration at full ripeness. Multivariate analysis confirmed strong genotype × maturity interactions shaping oil quality. Optimal harvest timing must therefore be tailored to each cultivar to maximize phenolic content, oxidative stability, and sensory excellence while balancing industrial yield. Full article

The integration of shape-memory alloy (SMA) wires into composite laminates offers a promising approach for active vibration damping. Towards this goal, this study investigates the damping behavior of hybrid random mat E-glass/epoxy composite plates with embedded SMA wires under electrically active and inactive conditions. The composites are tested using a Laser Doppler Vibrometer (LDV) and an impact hammer to assess the effect of SMA wire activation on the natural frequencies and vibration behavior of composites. For a fixed number of active SMA wires, differences in vibration behavior are evaluated between outer- and inner-wire activation configurations in both two-ply and four-ply composite plates. The results show that SMA wire activation significantly affects damping behavior, while the mode shapes remain unchanged. The magnitude and frequency of the first natural frequency as well as the quality factor (Q-factor) decrease in composites with activated SMA wires compared to the inactive configuration, indicating enhanced energy dissipation. Under the fully active condition, a reduction in vibrational amplitude of approximately 42–60% and a frequency shift of approximately 10–17% are observed. Compared to outer-wire activation, inner-wire activation results in greater reductions in vibration magnitude, reaching approximately 7–13%. Full article

Background/Objectives: SETD2 is a dual-function methyltransferase important for methylation of histone H3 at lysine 36 and α-tubulin in spindle microtubules. Genetic inactivation of SETD2 during oncogenesis drives loss of H3K36me3, genomic instability, and cancer progression. This study asked if disruption of genomic stability was a canonical feature of SETD2 inactivation via different pathways. Methods: We evaluated the impact of EPZ-719, a pharmacologic SETD2 inhibitor, and an H3.3K36M mutant histone (“oncohistone”) that binds and sequesters SETD2, on methylation activity and genomic stability in human cell lines. SETD2 activity was measured using in vitro methylation assays, H3K36me3 loss confirmed by Western analysis, and mitotic defects, specifically micronuclei and chromatin bridges, quantified with cytogenetic analysis. Results: EPZ-719 caused a dose- and time-dependent reduction in SETD2 activity on both histone and tubulin substrates, accompanied by significant increases in chromatin bridges and micronuclei in retinal pigmented epithelial (RPE-1) and 786-O ccRCC cells. Similarly, oncohistone expression markedly decreased SETD2 function, as determined by H3K36me3 levels, and induced comparable mitotic defects in 786-O cells, and aneuploidy in two chondrocyte cell lines expressing the H3.3K36M oncohistone. Combining EPZ-719 with H3.3K36M expression did not exacerbate mitotic defects beyond either oncohistone or pharmacologic inhibition alone, consistent with inhibition of SETD2 as their shared underlying mechanism of action. Conclusions: Pharmacologic inhibition and oncohistone-mediated sequestration of SETD2 converge on the induction of mitotic defects, underscoring SETD2’s essential role in maintaining genomic stability. Identification of loss of genomic stability as a canonical feature of SETD2 inactivation points to a potential therapeutic liability associated with targeting SETD2 in cancers where it is overexpressed and reveals a mechanism that could contribute to the progression of cancers expressing oncohistone mutations. Full article

Identifying environmental factors contributing to allergic asthma is critical for effective prevention. PM_2.5, a major environmental pollutant, and high relative humidity frequently coexist in urban and industrialized regions, particularly when ventilation is poor. However, the combined effects of PM_2.5 and humidity remain unclear. This study used a murine asthma model, exposing male Balb/c mice sensitized with ovalbumin (OVA) to PM_2.5 (75 μg/m³ and 35 μg/m³), based on China’s Ambient Air Quality Standards (GB3095-2012), and/or varying relative humidity levels in a controlled chamber. Allergic asthma severity was evaluated through histopathological changes, pulmonary function, Th1/Th2 balance, mucus hypersecretion, and inflammatory factor levels, alongside the activation of TRPV4 and MAPK signaling pathways (ERK, p38MAPK, and JNK). The results showed that high humidity (90%) or PM_2.5 exposure alone had minimal impact, but combined exposure to 75 μg/m³ PM_2.5 and 90% humidity markedly aggravated airway hyperresponsiveness, inflammation, and mucus hypersecretion. These changes coincided with enhanced TRPV4 activation and MAPK signaling, particularly p38MAPK and JNK, while ERK1/2 remained unaffected. A lower PM_2.5 concentration (35 μg/m³) combined with 90% humidity had a weaker impact. Blocking TRPV4 with HC-067047 effectively mitigated asthma exacerbation caused by combined exposure. These findings demonstrate that co-exposure to PM_2.5 and high humidity dose-dependently exacerbates allergic asthma, an effect likely mediated by TRPV4-MAPK pathway activation. Targeting TRPV4 may offer a potential therapeutic strategy to mitigate asthma exacerbation in environments with high humidity and PM_2.5. Full article

Micro- and nanoplastics (MNPs) are increasingly contaminating atmospheric particulates, yet their influence on PM_2.5 chemistry and toxicity remains poorly understood. This study investigates how secondary MNPs derived from common products (water bottles, coffee cups, and food plates) alter the properties of PM_2.5. We evaluated PM_2.5 leaching characteristics, oxidative potential, inflammatory activity, and bacterial-based cytological and metabolomic responses after 24 h of exposure to three MNP doses. MNPs markedly altered PM_2.5 chromophoric composition, with bottle-derived (PET) MNPs inducing the strongest increases in aromaticity, humification, and slope factor, followed by coffee cups (PLA/paper) and food plates (PP). These leaching shifts aligned with polymer-specific redox behaviors: bottle-derived MNPs enhanced antioxidant enrichment at high PM_2.5, whereas cup-derived MNPs produced the most pronounced protein-denaturation-based inflammatory activity. Escherichia coli assays showed non-linear growth responses, elevated reactive oxygen species, altered carbohydrate secretion, and membrane and protein perturbations that paralleled PM_2.5 chemical reactivity. FTIR metabolomic fingerprints revealed dose- and polymer-dependent disruptions in polysaccharide, lipid, and protein domains. Overall, the results demonstrate a mechanistic cascade in which MNP exposure reshapes PM_2.5 chemistry, amplifies oxidative and inflammatory potential, and culminates in measurable cytological and metabolic stress, with polymer identity (PET > PLA/paper > PP) as the dominant driver. Full article

Drones have long been explored by commercial and military users for supply. While several systems offering small payloads in drone delivery have seen operational use, large-scale supply drones have yet to be adopted. A range of setbacks cause this, including technological and operational challenges that hinder their adoption. Here, we evaluate these challenges from a conceptual modelling perspective and forecast their applicability once these barriers are overcome. This study uses technology trend modelling and bibliometric activity mapping methodologies to predict the applicability of specific technologies that are currently identified as operational challenges. Specifically for supply drones, we model trends in technological improvements of battery technology and aircraft control, and project its focus on landing zone autonomy and powertrain. The prediction also focuses on the current state of hybrid power and higher levels of automation required for landing zone operations. These models are validated through several published case studies of small delivery drones and then applied to assess the feasibility and constraints of larger supply drones. A case study involving the conceptual design of a supply drone large enough to move a shipping container is presented to illustrate the critical technologies required to transition large supply drones from concept to operational reality. Key technologies required for large-scale supply drones have yet to build up a critical mass of research activity, particularly on landing zone autonomy and powertrain. Moreover, additional constraints beyond technological and operational challenges could include limitations in autonomy, certification hurdles, regulatory complexity, and the need for greater social trust and acceptance. Full article