Search Results (533)

The aim of this study was to evaluate the effects of selenium (Se) biofortification on growth, biomass accumulation, and micronutrient composition of industrial hemp (Cannabis sativa L., cv. Finola) microgreens, with emphasis on Se uptake and its distribution among leaves, stems, and roots. Microgreens were subjected to four Se treatments (Se_0, Se_2, Se_4, and Se_6 µmol Se/L), and changes in morphological traits, micronutrient status (Mn, Fe, Cu, Zn), and Se accumulation were assessed. Selenium biofortification had a marked impact on plant morphology and biomass. Stem length decreased by 12–18% under Se treatments compared with the control, whereas root length increased slightly, particularly at Se_2 and Se_4 (up to +6%). Fresh industrial hemp microgreens biomass responded strongly to Se supply, with the highest stem, root, and total fresh mass recorded at Se_4—representing an increase of 15–22% relative to control plants. At the highest Se level (Se_6), biomass declined by approximately 10–14%, indicating potential growth inhibition at excessive Se concentrations. Micronutrient concentrations were significantly affected by Se. Leaf Mn increased from 152 mg kg⁻¹ at Se_0 to 175 mg kg⁻¹ at Se_6 (+15%), while leaf Zn decreased by 20–25% at higher Se exposure. Stems and roots showed similar antagonistic interactions, with Fe and Zn decreasing by up to 30% at elevated Se levels. Conversely, Mn in stems and roots increased with Se up to Se_4, reaching 400 mg kg⁻¹ in roots. Selenium accumulation exhibited a strong linear response to biofortification, with high coefficients of determination (R² = 0.9685–0.9943), confirming predictable and efficient Se uptake. Correlation analysis revealed strong positive associations among biomass-related traits and distinct interactions among micronutrients, especially the near-perfect correlation between Se and Cu in roots (r ≈ 0.99). Overall, industrial hemp microgreens demonstrate potential for selenium biofortification, provided that selenium application levels remain within safe dietary limits. Full article

The use of unconventional water sources, such as those from marine desalination plants, is challenging for agriculture due to boron concentrations exceeding 0.5 mg L⁻¹, which can impact crop yield and quality. To ensure sustainability, it is crucial to understand crop responses to high boron levels and to develop strategies to mitigate its toxic effects. This study evaluated the impact of irrigation with a nutrient solution containing 15 mg L⁻¹ of boron on tomato plants (Solanum lycopersicum L.). To modulate the physiological effects of boron toxicity, two biostimulant products based on an extract from the brown alga Laminaria digitata and other active ingredients were applied foliarly. Agronomic, nutritional, and metabolic parameters were analyzed, including total yield, number of fruits per plant, and fruit quality. Additionally, mineral analysis and metabolomic profiling of leaves and fruits were performed, focusing on amino acids, organic acids, sugars, and other metabolites. A control treatment was irrigated with a nutrient solution containing 0.25 mg L⁻¹ of boron. The results showed that a boron concentration of 15 mg L⁻¹ significantly reduced total yield by 45% and significantly decreased fruit size and firmness. Mineral and metabolomic analyses showed significant reductions in Mg and Ca concentrations, significant increases in P and Zn levels, excessive boron accumulation in leaves and fruits, and significant changes in metabolites associated with nitrogen metabolism and the Krebs cycle. Biostimulant application did not significantly improve agronomic performance, likely due to high boron accumulation in the leaves, although significant changes were detected in leaf nutritional status and metabolic profiles. Full article

Trace elements are essential for organisms, and their involvement in diverse diseases is increasingly recognised. Interest is increasing in veterinary medicine, particularly in relation to canine diseases. However, reference intervals for trace elements in dogs remain scarce. Plasma samples from 140 dogs were analysed by inductively coupled plasma mass spectrometry to determine the levels of 13 trace elements. Reference intervals (µg/L) were established for the following 12 elements: As, 0.417–8.17; Co, 0.039–1.33; Cr, 2.41–13.3; Cu, 296–790; Fe, 846–3643; Hg, 0.235–2.33; Ni, 0.567–9.04; Mn, 1.90–7.28; Mo, 1.43–12.7; Pb, 0.285–2.82; Se, 200–434; and Zn, 415–1095. However, Cd was below the limit of quantification in 77% of the samples. No differences in trace element concentrations were observed in relation to breed or reproductive status. Statistically significant differences were found in relation to sex (Cu, Mo, Zn), age (Co, Cu, Mo, Mn, Se, Zn), and size (Cu, Mo, Se, Zn); however, the magnitude of these effects varied among elements and was generally weak. Nevertheless, these factors should be considered when assessing trace element status. These reference intervals constitute an important resource for both clinical evaluation and future research. Full article

Background. The aim of this study was to develop a predictive model of anticancer drug therapy toxicity in patients with gastric cancer. Methods. The retrospective study included 100 patients with stage II–IV gastric cancer who underwent 4 chemotherapy cycles. Initial significant toxicity factors included age, gender, height, body mass, body mass index, disease stage, skeletal muscle index (SMI), as well as plasma levels of trace elements (copper, zinc, selenium, manganese) and thyroid-stimulating hormone, cancer histology type and treatment regimen. The CTCAE v5.0 scale was employed to assess the severity of adverse events. Statistical analysis and building of mathematical neural network models were carried out in SPSS Statistics (v19.0). Results. Lower SMI values were associated with higher rates of toxicity-related complications of anticancer drug therapy (p < 0.05): leukopenia, hypoproteinemia, nausea, vomiting, cardiovascular events. Anemia, thrombocytopenia, hepatic cytolysis syndrome, nausea, diarrhea, constipation and stomatitis showed a weaker correlation with SMI. An increase in TSH was associated with higher rates of thrombocytopenia, nausea and vomiting. A decrease in Cu/Zn in plasma correlated with the severity of leukopenia and diarrhea, whereas Se/Mn showed an inverse correlation with the severity of anemia. Conclusions. Sarcopenia, abnormal thyroid status and imbalances in copper, zinc, selenium and manganese in blood plasma of patients with gastric cancer may be used as predictors of increased toxicity of anticancer drug therapy. Full article

Tuber crops cultivated in basalt-derived soils are influenced by naturally high geochemical backgrounds, which may elevate heavy metal(loid) levels and associated health risks. To clarify the geochemical controls governing metal accumulation, this study analyzed rock, soil, and tuber (sweet potato and yam) samples from the Qiongbei volcanic area of Hainan Island, China. Concentrations of eight heavy metal(loid)s (As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) and 22 nutrient-related indicators (N, P, K, SOC, S, Se, Fe, Mn, and their available fractions) were determined. Soil contamination and potential human health risks were evaluated using the pollution index and the health risk model. The results showed that 11.1–55.6% of soil samples exceeded pollution thresholds for Cr, Cu, Ni, and Zn, reflecting typical basaltic high-background characteristics. In contrast, heavy metal(loid) concentrations in tuber crops were relatively low and jointly regulated by parent material composition and soil nutrient status. Non-carcinogenic risks (HI) were below 1, indicating acceptable exposure levels, while carcinogenic risks were mainly associated with Cd, Cr, and Pb, with total carcinogenic risk (TCR) exceeding 1 × 10⁻⁴, suggesting potential health concerns. Strong correlations between soil nutrients (N, P, K, SOC, S, Se, Mn, and Fe) and plant uptake of As, Cd, Cu, and Cr indicate that nutrient availability plays a crucial role in controlling heavy metal(loid) bioavailability. The volcanic soils exhibited a “high total content–low bioavailability” pattern. Enhancing soil Se, SOC, available N, and slowly available K (SAK) can effectively reduce Cd and other high-risk metal accumulation in tuber crops. These findings elucidate the key geochemical processes influencing heavy metal transfer in volcanic agroecosystems and provide a scientific basis for safe agricultural utilization and health risk prevention in high-background regions. Full article

Post-industrial heaps are a major environmental problem. They require remediation and reclamation, in which natural succession plays a key role in ecosystem development. This study aimed to assess the effect of heaps formed from materials of different origins on the nutrient content of silver birch (Betula pendula Roth), a pioneer species in this process. We analyzed nutrient contents in biomass fractions (fine and coarse roots, stemwood, bark, coarse and fine branches, leaves) and in soils sampled from 0 to 10, 10 to 20, 20 to 40, and 40 to 80 cm. Basic soil properties and the contents of N, P, K, Ca, Mg, S, Fe, Mn, Cu, and Zn in both soil and biomass were determined. The soils were poor in total organic carbon and differed in pH, texture, and nutrient status. Leaves and roots contained the highest nutrient contents, whereas stemwood contained the lowest. Statistical analyses revealed significant differences in all studied elements between heaps. Among macronutrients, N, P, and Mg were most abundant, followed by K, Ca, and S. Among micronutrients, Mn dominated, followed by Fe, Zn, and Cu. Findings underscore that silver birch growing on contaminated post-industrial heaps cannot be considered a hyperaccumulator of trace elements. Full article

Soil acidity severely constrains coffee production by reducing nutrient availability and promoting aluminum toxicity and oxidative stress. Foliar zinc oxide nanoparticles (ZnO NPs) have been proposed as redox modulators that can improve nutrient homeostasis under abiotic stress. However, the safe and effective range of Coffea arabica L. remains unclear. In this study, seedlings were grown in acidic soil and sprayed twice with ZnO NPs at 10, 25, 50, and 100 mg L⁻¹. Morphophysiological, biochemical, and ionomic parameters were evaluated fifty days after treatment. Moderate ZnO NPs doses led to intermediate stomatal conductance values, whereas net photosynthesis showed intermediate but non-significant responses only at 10–25 mg L⁻¹, with higher doses (50–100 mg L⁻¹) causing a marked decline. These doses did not significantly modify hydrogen peroxide (H₂O₂) or malondialdehyde (MDA) levels in leaves or roots. In contrast, the highest dose (100 mg L⁻¹) induced a marked increase in H₂O₂ without affecting MDA, indicating a partial oxidative response rather than clear lipid peroxidation. Foliar analysis showed that 50 mg L⁻¹ ZnO NPs significantly increased P compared with the optimal soil, while Ca and K remained statistically similar across treatments. Na in the optimal soil was comparable to the 10–25 mg L⁻¹ ZnO NPs treatments, whereas Na at 50–100 mg L⁻¹ ZnO NPs was significantly reduced and foliar Zn increased markedly with increasing nanoparticle dose. Proline accumulation reflected a dose-dependent osmotic adjustment, and chlorophyll ratios indicated adaptive photoprotection. Overall, foliar ZnO NPs mitigated acidity-induced stress through physiological and ionomic adjustment, with 10–25 mg L⁻¹ identified as the physiologically safe range for C. arabica seedlings grown under acidic conditions. Full article

In a rat model simulating moderate and relatively high human exposure to cadmium (Cd; 5 and 50 mg/L, respectively, for 12 months), it was examined whether zinc (Zn) supplementation (30 and 60 mg/L, increasing the daily intake of this element by 71% and 146%, respectively) could protect against this xenobiotic-caused disruption of the oxidative–reductive balance in the mandibular bone tissue and the subsequent oxidative damage to nucleic acids, proteins, and lipids. The exposure to Cd weakened the enzymatic antioxidative barrier (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)) and decreased the total antioxidative status (TAS) of this tissue. The treatment with Cd also increased the concentration of hydrogen peroxide (H₂O₂) and the total oxidative status (TOS) of the mandibular bone tissue, leading to the development of oxidative stress, as indicated by an elevated value of the oxidative stress index (OSI), and oxidative damage to nucleic acids, lipids, and proteins. Zn supplementation at 30 and 60 mg/L during Cd exposure at 5 and 50 mg/L effectively protected against the accumulation of this toxic heavy metal in mandibular bone tissue and prevented oxidative stress and oxidative modifications of nucleic acids, proteins, and lipids. In conclusion, increasing Zn intake by at least 71% during chronic oral exposure to Cd may prevent oxidative–antioxidative imbalance and the development of oxidative stress, thereby safeguarding cellular macromolecules in the mandibular bone tissue from oxidative damage. These findings highlight the potential role of Cd exposure in the aetiology of mandibular bone damage and provide the first evidence that Zn supplementation may represent an effective intervention to alleviate adverse impact of long-term oral exposure to Cd on mandibular bone. Full article

Herbicide treatment for agricultural crops may cause dramatic damage to production amount and quality. The aim of the present investigation was to compare different silicon formulations to assess their efficiency in maintaining faba bean plant growth with the herbicide spray Dicameron. Soil pollution due to Dicameron caused an intensive oxidant stress, decreasing bean pods, seed number and weight, antioxidant activity (AOA) and polyphenol content (TP), leaf chlorophyll, and carotene, sharply increasing proline level, and creating pod and leaf anomalies. All the Si formulations, i.e., ionic Si forms in the presence of microelements (Siliplant) or terpenes (BioSi), Si nanoparticles, and organic silicon adjuvant siloxane polyalkylene oxide (Atomic), significantly restored bean antioxidant status and leaf photosynthetic pigment accumulation, enhancing plant defense, as indicated by the proline level decrease. Only the ionic form of Si in the Siliplant formulation, containing essential microelements, facilitated the recovery of pod form and seed weight, while nano-Si was the most effective treatment for bean AOA restoration, and Atomic was the best in rebalancing chlorophyll and the worst in decreasing proline content. A strong beneficial effect of ionic Si in the presence of terpenes (BioSi) was recorded only on the yield of the control plants which did not undergo herbicide spraying. The results indicate a moderate beneficial effect of siloxane adjuvant on plant performance and antioxidant defense level and the highest positive impact on broad bean protection in response to the ionic Si (Siliplant formulation) supply also containing Cu, Zn, Mo, Mn, Fe, and B. Full article

In order to investigate the current status of soil heavy metal pollution, ecological risk, and risk sources in the black soil area of the Eastern Inner Mongolia Province, topsoil (0–20 cm) samples from farmland in the black soil area (N = 163) were collected to determine the contents of seven heavy metals. The levels of soil heavy metal pollution and ecological risk in the study area were evaluated by combining the geo-accumulation index, potential ecological risk index, and static environmental carrying capacity; the positive matrix factorization (PMF) model was used to identify the pollution sources and contributions of heavy metals in the soil and analyze the risk levels to adults and children. The soil was predominantly weakly acidic, with mean values of Cr, Ni, Cu, As, Cd, Pb, and Zn of 61.77, 26.77, 17.07, 12.11, 0.08, 12.61, and 85.71 mg·kg⁻¹. The mean concentrations of heavy metals exceeded the background values, except for Pb, the mean concentration of which was lower than the soil background. Ni concentrations of 6.21% at the sampling sites exceeded the risk screening value for agricultural soils. The geo-accumulation index showed that Cr (55.15%) and As (54.00%) were mainly mild pollutants; the static environmental carrying capacity indicated that the soils were slightly polluted by Ni, As, and Zn; and the potential ecological risk indices of Cd, Ni, and As were at moderate levels. The PMF model analyzed three pollution sources: mixed agricultural practice–transportation sources (39.46%), mineral-related activity sources (27.01%), and pesticide–fertilizer agricultural practices (33.53%). The human health risk assessment indicated that 46.58% of sampling sites posed a carcinogenic risk to children, with Ni as the main carcinogenic element. In conclusion, the potential contamination of As, Cd, Ni, Cr, and Zn in the Eastern Inner Mongolia farmland black soil area should be further studied. Full article

The incorporation of cattle slurry in soil in short-rotation-cycle poplar cultivations can be a win–win strategy, insofar as a main feedstock derived from local intensive dairy cattle breeding can be used as a natural fertilizer and in bioenergy produced in the same region. The circularity of this process can contribute to boosting local socio-economic value. In this context, this work involved the installation of a poplar SRC plantation with a density of 5330 trees ha⁻¹ in a 4000 m² moderately fertile flat site, which was formerly used as a vineyard. Mechanical dosages of slurry of 0, 26.6, 53.2, and 106.5 Mg ha⁻¹, designated as treatments T0, T1, T2, and T3, were applied three times per year during 2019, 2020, and 2021. The variables quantified were related to plant growth, biomass productivity and mass balances of K, P, Cu, Zn, Mg, and N, and organic matter in the whole soil, plant, and slurry system during the first rotation cycle. For treatments T0 and T1, all these seven chemical components showed positive balances in the system, with cumulative demand by soil and biomass being higher than cumulative supply by slurry. Negative balances occurred for P with T2 and T3 and for Zn with T3, so that an overall condition of nutrient saturation of the whole system was not achieved. A no-slurry application, or at most a moderate application equivalent to T1, in the second rotation cycle should therefore be prescribed to allow a nutrient equilibrium status to be achieved through internal seasonal recycling mechanisms. The biomass average productivities ranged from 6.1 to 11.8 Mg ha⁻¹ y⁻¹, peaking under treatment T2, and are within the typical values for a first rotation cycle for poplar SRCs. The biomass fuel quality was not affected by the slurry treatments. A good performance of plant total height and growth in diameter at breast height suggested that poplar trees were not stressed by the applied slurry. Only treatment T1 could assure that cattle CO₂-eq methane emissions were overall equilibrated by the carbon sequestration from poplar cultivation, with an absence of climatic-warming impacts. Treatments T2 and T3 could only partially minimize that impact, which would always exist. Globally, this site-specific analysis showed that, under moderately fertile conditions, controlled cattle slurry fertilization of poplar SRC cultivations, which would assure a long-term steady-state equilibrium, can be a viable option to contribute to decentralized production of bioenergy in rural communities. Full article

Marine sediments are a key component of aquatic ecosystems, linking diverse water uses, functions, and services. Chemical contamination of sediments is a global concern, with many jurisdictions striving to prevent future pollution and manage existing contamination. This study evaluates the contamination status of Rovinj’s coastal waters using an integrated approach that combines sediment and biota chemical analyses. Sediments were analyzed to assess long-term contaminant accumulation (D8.C1), while the Mediterranean mussel (Mytilus galloprovincialis) served as a bioindicator of bioavailable contaminants and their cumulative effects on marine habitats (D8.C2). Sediment samples were collected from five sites (S1–S5), and mussels were caged using Mussel Watch installations for approximately 120 days at a control site (Lim Bay) and within Rovinj harbor. Both matrices were analyzed for heavy metals (As, Cd, Cu, Cr, Hg, Ni, Pb, and Zn), polycyclic aromatic hydrocarbons (16 PAHs), and polychlorinated biphenyls (PCBs), following the EU Water Framework Directive. All sampled locations showed a reduction in sediment contamination relative to 2011 data, with most concentrations below ecotoxicological thresholds. Exceptions included elevated ΣPAH and PCB concentrations in the harbor (S1 = 3.18 mg/kg DW; 0.33 mg/kg DW) and marina (S2 = 3.64 mg/kg DW; 0.89 mg/kg DW), as well as Ni levels (S3 = 30 mg/kg DW; S4 = 34 mg/kg DW). Despite higher contaminant loads at some locations, mussel contaminant bioaccumulation remained limited, and their vitality and survival were only moderately affected in the harbor. Although localized increases in some contaminants were detected, all calculated Q_PECm values remained below 1.0, indicating no significant ecological risk. However, a moderate-to-high probability of toxic effects (P) may occur with long-term exposure for biota inhabiting harbor and marina areas. The results of this study demonstrate continued improvement in the environmental quality of Rovinj’s coastal waters compared to the previous decade. Full article

Cultivation practices such as fruit thinning and soil management with ground covers are commonly applied in Citrus orchards, yet their physiological impact on young trees remains poorly documented. This study evaluated the effects of manual fruit thinning and weed-control mesh on vegetative growth, fruit development, and leaf mineral composition of Citrus sinensis L. Osbeck cv. ‘Navelina’ grafted on Citrus macrophylla. A six-month field experiment was conducted in southeastern Spain under semi-arid Mediterranean conditions using six treatments that combined different soil coverage and subsurface drainage systems. After physiological fruit drop, trees were standardized to ten fruits per plant. Vegetative parameters (canopy and trunk dimensions), fruit growth (size, juice content), and foliar nutrient concentrations were monitored. Trees with ground cover showed significantly greater canopy expansion and juice yield compared to uncovered controls. A negative correlation between fruit number and canopy-to-fruit volume ratio highlighted the trade-off between vegetative vigor and fruit load. Foliar analysis revealed lower micronutrient concentrations (Fe, Mn, B, Zn) in uncovered trees, suggesting reduced nutritional status. These findings demonstrate that combining early thinning with weed-control mesh promotes vegetative vigor, improves juice yield, and enhances nutrient uptake, providing practical insights for optimizing orchard establishment and early Citrus productivity in water-limited environments. Full article

The intensification of agricultural practices and the consequent dramatic decrease in soil organic matter has increased the use of organic fertilizer to recover soil fertility and plant productivity. The aim of this study was to compare the effect of three amendments obtained from the recycling of urban and agri-food wastes on rhizosphere microbial community, soil, and plant nutrient status. The experiment was carried out on rhizobox-grown, 1-year-old vines of Sangiovese (Vitis vinifera L.), grafted onto 110 Richter (V. berlandieri × V. rupestris) planted in April 2023. Twenty-four rhizoboxes were filled with soil collected from a field trial in which three types of amendments had been applied since 2019. In detail, the complete randomized experimental design (with four replications) compared the following treatments: (1) municipal organic waste compost (ACM), (2) agri-food organic waste compost (ACF), (3) defecation gypsum (GDD), and (4) a control that received 60 kg of N ha⁻¹ year⁻¹ (CK). The application of the amendments increased the soil concentration of total C, total N, and pH. The application of ACM increases soil K and Zn and the concentration of N and K in plant roots. The application of all the amendments increased leaf N concentration in comparison with CK, but only ACF increased leaf P. ACM was the most effective in promoting microbial biodiversity, increasing phyla like Bacillota, Pseudomonata, and Bacteroidota, including genra like Bacillus, Neobacillus, Paenibacillus, and Pseudomonas. ACF promoted Nitrosospherota and Chitinophaga, and GDD promoted Chloroflexota and Agrobacterium. Full article

Background: Dromedary camel in Saudi Arabia thrive across diverse desert ecosystems where trace minerals are vital for key physiological functions, yet data on how regional and seasonal factors affect these minerals in dams and neonates are limited. Aim: This study investigated the effects of regional and seasonal variability on trace mineral status in dam serum (DS), dam milk (DM), and neonatal serum (NS) across major camel-rearing regions of Saudi Arabia. We hypothesized that environmental factors—particularly heat stress and local feed resources—drive regional and seasonal differences in mineral profiles and maternal–neonatal transfer. Methods: Samples of serum, milk, feed, water, and soil were collected from five major regions during three seasons. Concentrations of selenium (Se), zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), and iodine (I) were quantified, and correlations among biological compartments were analyzed. Meteorological data were used to compute the temperature-humidity index (THI). Results: The THI ranged from thermoneutral levels in the Northern winter (17.4) to severe heat stress in Eastern summer (33.8). Milk minerals exhibited strong seasonal and regional effects, with selenium peaking in summer and zinc in spring. Western dams showed elevated iron and iodine, whereas northern dams had higher zinc. Serum minerals in dams varied moderately with season but differed regionally for zinc, selenium, and iron. Neonatal serum reflected maternal and regional influences, showing significant season-by-region interactions for selenium and iodine. Positive correlations indicated coordinated maternal–neonatal mineral transfer, particularly for selenium, iodine, and zinc. Feed represented the main environmental source of Cu and Se. In conclusion, camel trace mineral status is mainly driven by environmental factors but regulated through maternal transfer, with selenium and iodine emerging as key heat-stress markers supporting targeted, region- and season-specific supplementation to improve health and productivity in arid regions. Full article