Search Results (9)

In clinical applications of surface-enhanced Raman spectroscopy (SERS) immunosensors, accurately determining analyte biomarker concentrations is essential. This study presents a non-invasive approach for quantifying various breast cancer biomarkers—including human epidermal growth factor receptor II (HER-II) (2+, 3+ (I), 3+ (II), 3+ (III), and positive IV) and CA 15-3—using a directional, plasmonically active, label-free SERS sensor. Each stage of sensor functionalization, conjugation, and biomarker interaction was verified by UV–Vis spectroscopy. Atomic force microscopy (AFM) characterized the morphology of gold nanourchin (GNU)-immobilized printed circuit board (PCB) substrates. An enhancement factor of ≈ 0.5 × 10⁵ was achieved using Rhodamine 6G as the probe molecule. Calibration curves were initially established using standard HER-II solutions at concentrations ranging from 1 to 100 ng/mL and CA 15-3 at concentrations from 10 to 100 U/mL. The SERS signal intensities in the 620–720 nm region were plotted against concentration, yielding linear sensitivity with R² values of 0.942 and 0.800 for HER-II and CA15-3, respectively. The same procedure was applied to breast cancer serum (BCS) samples, allowing unknown biomarker concentrations to be determined based on the corresponding calibration curves. SERS data were processed using the filtfilt filter from scipy.signal for smoothing and then baseline-corrected with the Improved Asymmetric Least Squares (IASLS) algorithm from the pybaselines.Whittaker library. Principal Component Analysis (PCA) effectively distinguished the sample groups and revealed spectral differences before and after biomarker interactions. Key Raman peaks were attributed to functional groups including N–H (primary and secondary amines), C–H antisymmetric stretching, C–N (amines), C=O antisymmetric stretching, NH₃⁺ (amines), carbohydrates, glycine, alanine, amides III, C=N stretches, and NH₂ in primary amides. Full article

In response to the limited research on silage maize classification in China and the lack of data support for refined agricultural and livestock management, this study proposes a Temporal Difference-based Silage Maize Identification Model (TempDiff-SMID) using the Google Earth Engine (GEE) platform. By analyzing the phenological phases of silage maize and grain maize, we identified their critical harvest periods and established decision rules for classifying silage maize, grain maize, and other land cover types. Preprocessed Sentinel-2 imagery was smoothed using the Whittaker filter to construct the TempDiff-SMID model. After iterative threshold optimization, the decision tree model achieved an overall accuracy of 0.9291 and a Kappa coefficient of 0.8923, indicating robust classification performance. The user’s accuracies for silage maize, grain maize, and other land cover types were 0.9216, 0.9219, and 0.9404, respectively, while the producer’s accuracies reached 0.94, 0.9008, and 0.9467, demonstrating minimal omission and commission errors across all categories. Furthermore, the F1 scores for silage maize, grain maize, and other land cover types were 0.9307, 0.9112, and 0.9435, respectively, confirming the effectiveness of the TempDiff-SMID framework in leveraging harvest time differences for accurate silage maize identification. To evaluate performance, we compared the TempDiff-SMID with the RF Model for Silage Maize Classification (SMRF). The TempDiff-SMID outperformed the SMRF in both overall accuracy (0.9043 vs. 0.9291) and Kappa coefficient (0.8511 vs. 0.8923), while also providing an intuitive representation of spectral and phenological differences between silage maize and grain maize. When applied to multi-year data, TempDiff-SMID demonstrated strong temporal transferability, achieving overall accuracies of 0.8621 (2022) and 0.8816 (2021), thereby confirming its robustness across growing seasons. The proposed model offers simplicity in methodology, clear interpretability, and efficient deployment, making it a practical tool for agricultural and livestock management systems. Its ability to rapidly adapt to new regions or years underscores its significance in supporting precision agriculture and sustainable farming practices. Full article

The Whittaker–Henderson (WH) graduation is a smoothing method for equally spaced one-dimensional data such as time series. It includes the Bohlmann filter, the Hodrick–Prescott (HP) filter, and the Whittaker graduation as special cases. Among them, the HP filter is the most prominent trend-cycle decomposition method for macroeconomic time series such as real gross domestic product. Recently, a modification of the HP filter, the boosted HP (bHP) filter, has been developed, and several studies have been conducted. The basic idea of the modification is to achieve more desirable smoothing by extracting long-term fluctuations remaining in the smoothing residuals. Inspired by the modification, this paper develops the boosted version of the WH graduation, which includes the bHP filter as a special case. Then, we establish its properties that are fundamental for applied work. To investigate the properties, we use a spectral decomposition of the penalty matrix of the WH graduation Full article

Operational crop monitoring applications, including crop type mapping, condition monitoring, and yield estimation, would benefit from the ability to robustly detect and map crop phenology measures related to the crop calendar and management activities like emergence, stem elongation, and harvest timing. However, this has proven to be challenging due to two main issues: first, the lack of optimised approaches for accurate crop phenology retrievals, and second, the cloud cover during the crop growth period, which hampers the use of optical data. Hence, in the current study, we outline a novel calibration procedure that optimises the settings to produce high-quality $NDVI$ time series as well as the thresholds for retrieving the start of the season (SOS) and end of the season (EOS) of different crops, making them more comparable and related to ground crop phenological measures. As a first step, we introduce a new method, termed UE-WS, to reconstruct high-quality $NDVI$ time series data by integrating a robust upper envelope detection technique with the Whittaker smoothing filter. The experimental results demonstrate that the new method can achieve satisfactory performance in reducing noise in the original $NDVI$ time series and producing high-quality $NDVI$ profiles. As a second step, a threshold optimisation approach was carried out for each phenophase of three crops (winter wheat, corn, and sugarbeet) using an optimisation framework, primarily leveraging the state-of-the-art hyperparameter optimization method (Optuna) by first narrowing down the search space for the threshold parameter and then applying a grid search to pinpoint the optimal value within this refined range. This process focused on minimising the error between the satellite-derived and observed days of the year (DOY) based on data from the German Meteorological Service (DWD) covering two years (2019–2020) and three federal states in Germany. The results of the calculation of the median of the temporal difference between the DOY observations of DWD phenology held out from a separate year (2021) and those derived from satellite data reveal that it typically ranged within ±10 days for almost all phenological phases. The validation results of the detection of dates of phenological phases against separate field-based phenological observations resulted in an $RMSE$ of less than 10 days and an R-squared value of approximately 0.9 or greater. The findings demonstrate how optimising the thresholds required for deriving crop-specific phenophases using high-quality $NDVI$ time series data could produce timely and spatially explicit phenological information at the field and crop levels. Full article

Normalized difference vegetation index (NDVI) time series data, derived from optical images, play a crucial role for crop mapping and growth monitoring. Nevertheless, optical images frequently exhibit spatial and temporal discontinuities due to cloudy and rainy weather conditions. Existing algorithms for reconstructing NDVI time series using multi-source remote sensing data still face several challenges. In this study, we proposed a novel method, an enhanced gap-filling and Whittaker smoothing (EGF-WS), to reconstruct NDVI time series (EGF-NDVI) using Google Earth Engine. In EGF-WS, NDVI calculated from MODIS, Landsat-8, and Sentinel-2 satellites were combined to generate high-resolution and continuous NDVI time series data. The MODIS NDVI was employed as reference data to fill missing pixels in the Sentinel–Landsat NDVI (SL-NDVI) using the gap-filling method. Subsequently, the filled NDVI was smoothed using a Whittaker smoothing filter to reduce residual noise in the SL-NDVI time series. With reference to the all-round performance assessment (APA) metrics, the performance of EGF-WS was compared with the conventional gap-filling and Savitzky–Golay filter approach (GF-SG) in Fusui County of Guangxi Zhuang Autonomous Region. The experimental results have demonstrated that the EGF-WS can capture more accurate spatial details compared with GF-SG. Moreover, EGF-NDVI of Fusui County exhibited a low root mean square error (RMSE) and a high coefficient of determination (R²). In conclusion, EGF-WS holds significant promise in providing NDVI time series images with a spatial resolution of 10 m and a temporal resolution of 8 days, thereby benefiting crop mapping, land use change monitoring, and various ecosystems, among other applications. Full article

Accurate evaluation of start of season (SOS) changes is essential to assess the ecosystem’s response to climate change. Smoothing method is an understudied factor that can lead to great uncertainties in SOS extraction, and the applicable situation for different smoothing methods and the impact of smoothing parameters on SOS extraction accuracy are of critical importance to be clarified. In this paper, we use MOD13Q1 normalized difference vegetation index (NDVI) data and SOS observations from eight agrometeorological stations on the Qinghai–Tibetan Plateau (QTP) during 2001–2011 to compare the SOS extraction accuracies of six popular smoothing methods (Changing Weight (CW), Savitzky-Golay (SG), Asymmetric Gaussian (AG), Double-logistic (DL), Whittaker Smoother (WS) and Harmonic Analysis of NDVI Time-Series (HANTS)) for two types of different SOS extraction methods (dynamic threshold (DT) with 9 different thresholds and double logistic (Zhang)). Furthermore, a parameter sensitivity analysis for each smoothing method is performed to quantify the impacts of smoothing parameters on SOS extraction. Finally, the suggested smoothing methods and reference ranges for the parameters of different smoothing methods were given for grassland phenology extraction on the QTP. The main conclusions are as follows: (1) the smoothing methods and SOS extraction methods jointly determine the SOS extraction accuracy, and a bad denoising performance of smoothing method does not necessarily lead to a low SOS extraction accuracy; (2) the default parameters for most smoothing methods can result in acceptable SOS extraction accuracies, but for some smoothing methods (e.g., WS) a parameter optimization is necessary, and the optimal parameters of the smoothing method can increase the R² and reduce the RMSE of SOS extraction by up to 25% and 331%; (3) The main influencing factor of the SOS extraction using the DT method is the stability of the minimum value in the NDVI curve, and for the Zhang method the curve shape before the peak of the NDVI curve impacts the most; (4) HANTS is the most stable method no matter with (fitness = 35.05) or without parameter optimization (fitness = 33.52), which is recommended for QTP grassland SOS extraction. The findings of this study imply that remote sensing-based vegetation phenology extraction can be highly uncertain, and a careful selection and parameterization of the time-series smoothing method should be taken to achieve an accurate result. Full article

Time series land cover data statistics often fluctuate abruptly due to seasonal impact and other noise in the input image. Temporal smoothing techniques are used to reduce the noise in time series data used in land cover mapping. The effects of smoothing may vary based on the smoothing method and land cover category. In this study, we compared the performance of Fourier transformation smoothing, Whittaker smoother and Linear-Fit averaging smoother on Landsat 5, 7 and 8 based yearly composites to classify land cover in Province No. 1 of Nepal. The performance of each smoother was tested based on whether it was applied on image composites or on land cover primitives generated using the random forest machine learning method. The land cover data used in the study was from the years 2000 to 2018. Probability distribution was examined to check the quality of primitives and accuracy of the final land cover maps were accessed. The best results were found for the Whittaker smoothing for stable classes and Fourier smoothing for other classes. The results also show that classification using a properly selected smoothing algorithm outperforms a classification based on its unsmoothed data set. The final land cover generated by combining the best results obtained from different smoothing approaches increased our overall land cover map accuracy from 79.18% to 83.44%. This study shows that smoothing can result in a substantial increase in the quality of the results and that the smoothing approach should be carefully considered for each land cover class. Full article

Mapping forest composition is a major concern for forest management, biodiversity assessment and for understanding the potential impacts of climate change on tree species distribution. In this study, the suitability of a dense high spatial resolution multispectral Formosat-2 satellite image time-series (SITS) to discriminate tree species in temperate forests is investigated. Based on a 17-date SITS acquired across one year, thirteen major tree species (8 broadleaves and 5 conifers) are classified in a study area of southwest France. The performance of parametric (GMM) and nonparametric (k-NN, RF, SVM) methods are compared at three class hierarchy levels for different versions of the SITS: (i) a smoothed noise-free version based on the Whittaker smoother; (ii) a non-smoothed cloudy version including all the dates; (iii) a non-smoothed noise-free version including only 14 dates. Noise refers to pixels contaminated by clouds and cloud shadows. The results of the 108 distinct classifications show a very high suitability of the SITS to identify the forest tree species based on phenological differences (average $\kappa =0.93$ estimated by cross-validation based on 1235 field-collected plots). SVM is found to be the best classifier with very close results from the other classifiers. No clear benefit of removing noise by smoothing can be observed. Classification accuracy is even improved using the non-smoothed cloudy version of the SITS compared to the 14 cloud-free image time series. However conclusions of the results need to be considered with caution because of possible overfitting. Disagreements also appear between the maps produced by the classifiers for complex mixed forests, suggesting a higher classification uncertainty in these contexts. Our findings suggest that time-series data can be a good alternative to hyperspectral data for mapping forest types. It also demonstrates the potential contribution of the recently launched Sentinel-2 satellite for studying forest ecosystems. Full article

More than 20 techniques have been developed to de-noise time-series vegetation index data from different satellite sensors to reconstruct long time-series data sets. Although many studies have compared Normalized Difference Vegetation Index (NDVI) noise-reduction techniques, few studies have compared these techniques systematically and comprehensively. This study tested eight techniques for smoothing different vegetation types using different types of multi-temporal NDVI data (Advanced Very High Resolution Radiometer (AVHRR) (Global Inventory Modeling and Map Studies (GIMMS) and Pathfinder AVHRR Land (PAL), Satellite Pour l’ Observation de la Terre (SPOT) VEGETATION (VGT), and Moderate Resolution Imaging Spectroradiometer (MODIS) (Terra)) with the ultimate purpose of determining the best reconstruction technique for each type of vegetation captured with four satellite sensors. These techniques include the modified best index slope extraction (M-BISE) technique, the Savitzky-Golay (S-G) technique, the mean value iteration filter (MVI) technique, the asymmetric Gaussian (A-G) technique, the double logistic (D-L) technique, the changing-weight filter (CW) technique, the interpolation for data reconstruction (IDR) technique, and the Whittaker smoother (WS) technique. These techniques were evaluated by calculating the root mean square error (RMSE), the Akaike Information Criterion (AIC), and the Bayesian Information Criterion (BIC). The results indicate that the S-G, CW, and WS techniques perform better than the other tested techniques, while the IDR, M-BISE, and MVI techniques performed worse than the other techniques. The best de-noise technique varies with different vegetation types and NDVI data sources. The S-G performs best in most situations. In addition, the CW and WS are effective techniques that were exceeded only by the S-G technique. The assessment results are consistent in terms of the three evaluation indexes for GIMMS, PAL, and SPOT data in the study area, but not for the MODIS data. The study will be very helpful for choosing reconstruction techniques for long time-series data sets. Full article