Search Results (60)

African hydrological systems are incredibly complex and highly sensitive to climate variability. This review synthesizes observational data, remote sensing, and climate modeling to understand the interactions between fluvial processes, water cycle dynamics, and anthropogenic pressures. Currently, these systems are experiencing accelerating warming (+0.3 °C/decade), leading to more intense hydrological extremes and regionally varied responses. For example, East Africa has shown reversed temperature–moisture correlations since the Holocene onset, while West African rivers demonstrate nonlinear runoff sensitivity (a threefold reduction per unit decline in rainfall). Land-use and land-cover changes (LULCC) are as impactful as climate change, with analysis from 1959–2014 revealing extensive conversion of primary non-forest land and a more than sixfold increase in the intensity of pastureland expansion by the early 21st century. Future projections, exemplified by studies in basins like Ethiopia’s Gilgel Gibe and Ghana’s Vea, indicate escalating aridity with significant reductions in surface runoff and groundwater recharge, increasing aquifer stress. These findings underscore the need for integrated adaptation strategies that leverage remote sensing, nature-based solutions, and transboundary governance to build resilient water futures across Africa’s diverse basins. Full article

The development of resource-efficient digital technologies is a critical challenge in the wastewater sector. This industrial case study, conducted in collaboration with the Veas Water Resource Recovery Facility in Norway, focused on creating data pre-processing methods and resource-efficient control strategies. Using data from the Veas biogas plant, dynamic models were developed to compare control outcomes. The primary objective was to maximize biogas production and hot water usage while maintaining optimal temperature and hydraulic retention time by adjusting inlet sludge and hot water flow rates. Sequential operations were approximated as continuous operations using a 30-min moving minimum/maximum for bimodal data and a 2-h moving average for noisy data. The data-driven dynamic models achieved an accuracy of up to $R^2$ of 0.85. The control strategy, which included one feedback controller, one ratio controller, and flow rate restrictions, was compared to real production data (baseline) and tested across six scenarios. The best improvement over the baseline scenario resulted in a 3% increase in total biogas production, a 6% increase in total organic loading, a 13% increase in hot water use, and a one-day reduction in hydraulic retention time. Future work should focus on control studies using extended datasets and nonlinear models. Full article

This study investigates the application of an eNose (electrochemical sensory array) device as a rapid and cost-effective screening tool to detect increasingly prevalent counterfeit electronic cigarettes, and those to which potentially hazardous excipients such as vitamin E acetate (VEA) have been added, without the need to generate and test the aerosol such products are intended to emit. A portable, in-field screening tool would also allow government officials to swiftly identify adulterated electronic cigarette e-liquids containing illicit flavorings such as menthol. Our approach involved developing canonical discriminant analysis (CDA) models to differentiate formulation components, including e-liquid bases and nicotine, which the eNose accurately identified. Additionally, models were created using e-liquid bases adulterated with menthol and VEA. The eNose and CDA model correctly identified menthol-containing e-liquids in all instances but were only able to identify VEA in 66.6% of cases. To demonstrate the applicability of this model to a commercial product, a Virginia Tobacco JUUL product was adulterated with menthol and VEA. A CDA model was constructed and, when tested against the prediction set, it was able to identify samples adulterated with menthol 91.6% of the time and those containing VEA in 75% of attempts. To test the ability of this approach to distinguish commercial e-liquid brands, a model using six commercial products was generated and tested against randomized samples on the same day as model creation. The CDA model had a cross-validation of 91.7%. When randomized samples were presented to the model on different days, cross-validation fell to 41.7%, suggesting that interday variability was problematic. However, a subsequently developed support vector machine (SVM) identification algorithm was deployed, increasing the cross-validation to 84.7%. A prediction set was challenged against this model, yielding an accuracy of 94.4%. Altered Elf Bar and Hyde IQ formulations were used to simulate counterfeit products, and in all cases, the brand identification model did not classify these samples as their reference product. This study demonstrates the eNose’s capability to distinguish between various odors emitted from e-liquids, highlighting its potential to identify counterfeit and adulterated products in the field without the need to generate and test the aerosol emitted from an electronic cigarette. Full article

Fungi are rich sources of secondary metabolites of agrochemical, pharmaceutical, and food importance, such as mycotoxins, antibiotics, and antitumor agents. Secondary metabolites play vital roles in fungal pathogenesis, growth and development, oxidative status modulation, and adaptation/resistance to various environmental stresses. LaeA contains an S-adenosylmethionine binding site and displays methyltransferase activity. The members of velvet proteins include VeA, VelB, VelC, VelD and VosA for each member with a velvet domain. LaeA and velvet proteins can form multimeric complexes such as VosA-VelB and VelB-VeA-LaeA. They belong to global regulators and are mainly impacted by light. One of their most important functions is to regulate gene expressions that are responsible for secondary metabolite biosynthesis. The aim of this mini-review is to represent the newest cognition of the biosynthetic regulation of mycotoxins and other fungal secondary metabolites by LaeA and velvet proteins. In most cases, LaeA and velvet proteins positively regulate production of fungal secondary metabolites. The regulated fungal species mainly belong to the toxigenic fungi from the genera of Alternaria, Aspergillus, Botrytis, Fusarium, Magnaporthe, Monascus, and Penicillium for the production of mycotoxins. We can control secondary metabolite production to inhibit the production of harmful mycotoxins while promoting the production of useful metabolites by global regulation of LaeA and velvet proteins in fungi. Furthermore, the regulation by LaeA and velvet proteins should be a practical strategy in activating silent biosynthetic gene clusters (BGCs) in fungi to obtain previously undiscovered metabolites. Full article

This paper explores the complex relationship between energy poverty and the maintenance of democratic values within the European Union (EU), suggesting that energy poverty not only impacts economic stability and health outcomes but also poses significant challenges to democratic engagement and equity. To measure energy poverty, a composite index is developed using the entropy method, which surpasses traditional measures focused solely on access to energy or its developmental implications. To assess the level of democratic governance in EU countries, the voice and accountability index (VEA), which is part of the World Governance Indicators compiled by the World Bank, is utilized. By analyzing EU data from 2006 to 2022, the findings suggest that a 1% improvement in VEA quality, represented by a coefficient of 0.122, is correlated with a notable improvement in the energy poverty index. This suggests that the EU should focus on enhancing transparency and public participation in energy decision-making, along with ensuring accountability in policy implementation. The research also differentiates between full and flawed democracies, noting that tailored approaches are needed. In full democracies, leveraging economic prosperity and trade is crucial due to their significant positive impacts on the energy poverty index. In contrast, in flawed democracies, enhancing governance and accountability is more impactful, as evidenced by a higher coefficient of 0.193. Strengthening legal and regulatory frameworks, improving regulatory quality, and ensuring public engagement in governance could substantially mitigate energy poverty in these contexts. In addition, this paper demonstrates that this relationship is influenced by factors such as income inequality, energy intensity, and trade openness. Full article

Velvet (VeA), a light-regulated protein that shuttles between the cytoplasm and the nucleus, serves as a key global regulator of secondary metabolism in various Aspergillus species and plays a pivotal role in controlling multiple developmental processes. The gene vepN was chosen for further investigation through CHIP-seq analysis due to significant alterations in its interaction with VeA under varying conditions. This gene (AFLA_006970) contains a Septin-type guanine nucleotide-binding (G) domain, which has not been previously reported in Aspergillus flavus (A. flavus). The functional role of vepN in A. flavus was elucidated through the creation of a gene knockout mutant and a gene overexpression strain using a well-established dual-crossover recombinational technique. A comparison between the wild type (WT) and the ΔvepN mutant revealed distinct differences in morphology, reproductive capacity, colonization efficiency, and aflatoxin production. The mutant displayed reduced growth rate; dispersion of conidial heads; impaired cell wall integrity; and decreased sclerotia formation, colonization capacity, and aflatoxin levels. Notably, ΔvepN exhibited complete growth inhibition under specific stress conditions, highlighting the essential role of vepN in A. flavus. This study provides evidence that vepN positively influences aflatoxin production, morphological development, and pathogenicity in A. flavus. Full article

The discovery of new genes with novel functions is a major driver of adaptive evolutionary innovation in plants. Especially in woody plants, due to genome expansion, new genes evolve to regulate the processes of growth and development. In this study, we characterized the unique VeA transcription factor family in Populus alba × Populus glandulosa, which is associated with secondary metabolism. Twenty VeA genes were characterized systematically on their phylogeny, genomic distribution, gene structure and conserved motif, promoter binding site, and expression profiling. Furthermore, through ChIP-qPCR, Y1H, and effector-reporter assays, it was demonstrated that PagMYB128 directly regulated PagVeA3 to influence the biosynthesis of secondary metabolites. These results provide a basis for further elucidating the function of VeAs gene in poplar and its genetic regulation mechanism. Full article

Aspergillus fumigatus is the leading cause of aspergillosis, associated with high mortality rates, particularly in immunocompromised individuals. In search of novel genetic targets against aspergillosis, we studied the WOPR transcription factor OsaA. The deletion of the osaA gene resulted in colony growth reduction. Conidiation is also influenced by osaA; both osaA deletion and overexpression resulted in a decrease in spore production. Wild-type expression levels of osaA are necessary for the expression of the conidiation regulatory genes brlA, abaA, and wetA. In addition, osaA is necessary for normal cell wall integrity. Furthermore, the deletion of osaA resulted in a reduction in the ability of A. fumigatus to adhere to surfaces, decreased thermotolerance, as well as increased sensitivity to oxidative stress. Metabolomics analysis indicated that osaA deletion or overexpression led to alterations in the production of multiple secondary metabolites, including gliotoxin. This was accompanied by changes in the expression of genes in the corresponding secondary metabolite gene clusters. These effects could be, at least in part, due to the observed reduction in the expression levels of the veA and laeA global regulators when the osaA locus was altered. Importantly, our study shows that osaA is indispensable for virulence in both neutropenic and corticosteroid-immunosuppressed mouse models. Full article

Penicillium expansum is one the major postharvest pathogens of pome fruit during postharvest handling and storage. This fungus also produces patulin, which is a highly toxic mycotoxin that can contaminate infected fruits and their derived products and whose levels are regulated in many countries. In this study, we investigated the biocontrol potential of non-mycotoxigenic strains of Penicillium expansum against a mycotoxigenic strain. We analyzed the competitive behavior of two knockout mutants that were unable to produce patulin. The first mutant (∆patK) involved the deletion of the patK gene, which is the initial gene in patulin biosynthesis. The second mutant (∆veA) involved the deletion of veA, which is a global regulator of primary and secondary metabolism. At the phenotypic level, the ∆patK mutant exhibited similar phenotypic characteristics to the wild-type strain. In contrast, the ∆veA mutant displayed altered growth characteristics compared with the wild type, including reduced conidiation and abnormal conidiophores. Neither mutant produced patulin under the tested conditions. Under various stress conditions, the ∆veA mutants exhibited reduced growth and conidiation when exposed to stressors, including cell membrane stress, oxidative stress, osmotic stress, and different pH values. However, no significant changes were observed in the ∆patK mutant. In competitive growth experiments, the presence of non-mycotoxigenic strains reduced the population of the wild-type strain during in vitro growth. Furthermore, the addition of either of the non-mycotoxigenic strains resulted in a significant decrease in patulin levels. Overall, our results suggest the potential use of non-mycotoxigenic mutants, particularly ∆patK mutants, as biocontrol agents to reduce patulin contamination in food and feed. Full article

A new Range Equation for a hybrid-electric propeller-driven aircraft was formulated by an original derivation based on the comparison of Virtual Electrical Aircraft (VEA) and Virtual Thermal Aircraft (VTA) range equations. The new formulation makes it possible to study the range of a hybrid aircraft with pre-established values of electric motor usage rate. The fuel and battery mass are defined "a priori", and do not depend on the power split, so even the aircraft’s total mass is constant. The comparison with the typical range formulas available for hybrid aircraft was made on the basis of a reference composite VLA category aircraft manufactured by the CFM Air company. The analysis carried out shows that there is an optimum hybridization level as a function of the pre-set specific energy of the batteries system. Full article

Aflatoxin B1 (AFB1) is the most potent naturally occurring carcinogen for humans and animals produced by the common fungus Aspergillus flavus (A. flavus). Aflatoxin (AF) contamination in commodities is a global concern related to the safety of food and feed, and it also impacts the agricultural economy. In this study, we investigated the AFB1-inhibiting activity of a new benzaldehyde derivative, 2-[(2-methylpyridin-3-yl)oxy]benzaldehyde (MPOBA), on A. flavus. It was found that MPOBA inhibited the production of AFB1 by A. flavus, with an IC₅₀ value of 0.55 mM. Moreover, the inhibition of conidiation was also observed at the same concentration. The addition of MPOBA resulted in decreased transcript levels of the aflR gene, which encodes a key regulatory protein for the biosynthesis of AF, and also decreased transcript levels of the global regulator genes veA and laeA. These results suggested that MPOBA has an effect on the regulatory mechanism of the development and differentiation of conidia, leading to the inhibition of AFB1 production. In addition, the cytotoxicity study showed that MPOBA had a very low cytotoxic effect on the Madin-Darby canine kidney (MDCK) cell line. Therefore, MPOBA may be a potential compound for developing practically effective agents to control AF contamination. Full article

Distillation is widely recognized as the preferred method for separation due to its operational and control benefits. Traditional distillation processes, however, cannot successfully separate azeotropic mixtures with near boiling points. Numerous special distillation processes have been developed to address this limitation. Extractive distillation, in particular, has gained significant popularity in the chemical, petrochemical, pharmaceutical, and refining industries. This review examined the state-of-the-art advances in extractive distillation. The importance of the proper selection of a solvent was discussed. Several configurations of extractive distillation processes were presented. Additionally, alternative extractive distillation systems have been elaborated. However, significant research gaps remain, such as the need for an exhaustive investigation of various control variables, the impact of certain entrainers on distillation processes, and cost comparisons across specialized distillation systems. Furthermore, process intensification strategies require additional research to solve complexity and operability issues. The integration of energy-efficient technologies, developments in renewable energy consumption, and the development of cost-effective reactive or split distillation columns will shape the future of distillation operations. These advances will help the chemical process sector achieve improved energy efficiency, lower environmental impact, and increased sustainability. Full article

This work describes the cooptation/grammaticalization process developed by the expression no veas. The hypothesis it defends, endorsed by previous research, considers that in this process, this expression appears in different constructions, originally integrated in the clause or peripheral, and is involved in a process of fixation towards pragmatic markers. The parenthetical distribution is fundamental to this subjectification and constructionalizationss. In some cases, the cooptation process has not ended, and constructions can act as semi-fixed patterns. These constructions provide procedural content but, at the same time, allow lexical choice in part of their structure. In other cases, we are already dealing with fixed markers. The Spanish structure no veas can appear as a free verbal construction (“No veas tanto la televisión” (don’t watch television so much)), as a semi-free intensification construction (“no veas lo enfadado que está” (you wouldn’t believe how angry he is), “está gritando que no veas” (he’s shouting like you wouldn’t believe)), or as a discourse operator (“El campo está lleno, no veas” (the stadium’s full, unbelievable)). In the latter case, the structure appears peripheral and displays a high degree of mobility (it could be inserted at the beginning of the utterance or appear in an intermediate position, not just at the end) and functions as a modal operator of surprise (a mirative) or a comment with intensifying meaning. Full article

Self-compacting concrete (SCC) production is a complex operation that requires finding a good combination and suitable dosages for its constituents. Several formulation methods have been developed to meet the workability requirements of SCC. Mortar spread is used to estimate SCC’s rheological properties, but the use of supplementary cementitious materials, such as metakaolin, could affect the accuracy of the estimation. In this paper, the relationships between the fresh properties of local-metakaolin (MK)-based SCC and the spreading of its mortar portion were investigated. The results showed the existence of good correlations between the spreading of mortar portion of SCC and its fresh state properties. The partial substitution of cement with MK did not affect these correlations. The mortar flow should be chosen according to the required rheological properties of the SCC. This can be achieved by using an appropriate viscosity-enhancing agent (VEA). Full article

The Attentional Networks Test for Interactions and Vigilance—executive and arousal components (ANTI-Vea) is a computerized task of 32 min duration in the standard format. The task simultaneously assesses the main effects and interactions of the three attentional networks (i.e., phasic alertness, orienting, and executive control) and two dissociated components of vigilance with reasonable reliability (executive and arousal vigilance). We present this free and publicly accessible resource (ANTI-Vea-UGR; https://anti-vea.ugr.es/) developed to easily run, collect, and analyze data with the ANTI-Vea (or its subtasks measuring some attentional and/or vigilance components embedded in the ANTI-Vea). Available in six different languages, the platform allows for the adaptation of stimulus timing and procedure to facilitate data collection from different populations (e.g., clinical patients, children). Collected data can be freely downloaded and easily analyzed with the provided scripts and tools, including a Shiny app. We discuss previous evidence supporting that attention and vigilance components can be assessed in typical lab conditions as well as online and outside the laboratory. We hope this tutorial will help researchers interested in measuring attention and vigilance with a tool useful to collect data from large sample sizes and easy to use in applied contexts. Full article