Search Results (54)

The present study aimed to evaluate the impact of two dietary regimens, characterized by different forage-to-concentrate ratios, on feed intake, milk yield and composition, as well as economic and environmental sustainability in lactating buffalo cows. Group H was fed a high-forage diet, while Group L was fed a diet with a lower forage-to-concentrate ratio. Despite a lower energy value (Unit for Feed Lactation: UFL 0.89 vs. 0.91), the diet of group H met the animals’ nutritional requirements, with similar dry matter intake (DMI) and body condition scores observed in both groups. While there was no significant difference in milk yield, protein, and lactose, group H exhibited significantly higher milk fat content and an enhanced fatty acid profile, characterized by increased oleic acid, conjugated linoleic acids (CLAs), and omega-3 polyunsaturated fatty acids. These fatty acids have been linked to potential health benefits in humans, including anti-inflammatory, cardioprotective, and anticarcinogenic effects. From an economic perspective, the H group’s diet was found to be more cost-effective, resulting in EUR 0.46 savings per head per day and a higher yield of mozzarella cheese. From an environmental perspective, the high-forage diet reduced reliance on imported concentrates and irrigation-dependent crops, aligning with sustainable development goals. In conclusion, increasing the forage content in buffalo diets has been demonstrated to support animal performance, improve milk quality, reduce environmental impact, and enhance economic returns for producers. Full article

Background: Detrusor underactivity (DU) and bladder outlet obstruction (BOO) are common causes of voiding dysfunction in women with lower urinary tract symptoms (LUTS). However, differentiating between them remains challenging due to overlapping clinical presentations and a reliance on invasive urodynamic studies (UDS). This study aimed to develop a non-invasive, office-based clinical prediction model to distinguish DU from BOO in women with non-neurogenic LUTS. Methods: We conducted a retrospective analysis of 88 women who underwent pressure-flow studies at two outpatient clinics between 2012 and 2022. DU was defined using a projected isovolumetric pressure 1 (PIP1) < 30 cm H₂O, and BOO was defined by a Female-Specific Bladder Outlet Obstruction Index (BOOIf) > 18. Clinical symptoms, uroflowmetry (UFL) parameters, and pelvic organ prolapse staging (POP-Q) were evaluated. A multivariate logistic regression model was constructed using a stepwise selection procedure. Results: Of the 88 patients, 38 (43.2%) were diagnosed with DU and 50 (56.8%) with BOO. Four predictors were retained in the final model: hesitancy (OR = 2.06, p = 0.18), incomplete emptying (OR = 3.52, p = 0.02), POP-Q < 3 (OR = 0.15, p = 0.02), and longer time to Qmax on UFL (OR = 1.05, p = 0.004). The model achieved a Harrell’s Concordance Index (C-index) of 0.779. Using a probability cutoff of 0.3, the model demonstrated a sensitivity of 86.8%, specificity of 46.0%, positive predictive value of 55.0%, and negative predictive value of 82.1%. Conclusions: We present a novel non-invasive prediction model incorporating clinical symptoms, UFL metrics, and pelvic exam findings that may aid in differentiating DU from BOO in women with LUTS. Full article

The facility location problem is a classical combinatorial optimization problem with extensive applications spanning communication technology, economic management, traffic governance, and public services. The facility location problem is to assign a set of clients to a set of facilities such that each client connects to a facility and the total cost (open cost and connection cost) is as low as possible. Among its various models, the uncapacitated facility location (UFL) problem is the most fundamental and widely studied. However, in real-world scenarios, resource constraints often make the UFL problem insufficient, necessitating more generalized models. This investigation primarily focuses on the universal facility location (Uni-FL) problem, a generalized framework encompassing both capacitated facility location problems (with hard and soft capacity constraints) and the UFL problem. Through a systematic analysis, we examine the Uni-FL problem alongside its specialized variants: the hard capacitated facility location (HCFL) problem and soft capacitated facility location (SCFL) problem. A comprehensive survey is conducted of existing approximation algorithms and theoretical results. The relevant results of their important variants are also discussed. In addition, we propose some open questions and future research directions for this problem based on existing research. Full article

Background/Objectives: Observational studies suggest a link between vascular calcification and dementia or cognitive decline, but the evidence is conflicting, and the underlying mechanisms are unclear. Here, we investigate the shared genetic and causal relationships of vascular calcification—coronary artery calcification (CAC) and abdominal aortic calcification (AAC)—with Alzheimer’s disease (AD), and five cognitive traits. Methods: We analyse large-scale genome-wide association studies (GWAS) summary statistics, using well-regarded methods, including linkage disequilibrium score regression (LDSC), Mendelian randomisation (MR), pairwise GWAS (GWAS-PW), and gene-based association analysis. Results: Our findings reveal a nominally significant positive genome-wide genetic correlation between CAC and AD, which becomes non-significant after excluding the APOE region. CAC and AAC demonstrate significant negative correlations with cognitive performance and educational attainment. MR found no causal association between CAC or AAC and AD or cognitive traits, except for a bidirectional borderline-significant association between AAC and fluid intelligence scores. Pairwise-GWAS analysis identifies no shared causal SNPs (posterior probability of association [PPA]3 < 0.5). However, we find pleiotropic loci (PPA4 > 0.9), particularly on chromosome 19, with gene association analyses revealing significant genes in shared regions, including APOE, TOMM40, NECTIN2, and APOC1. Moreover, we identify suggestively significant loci (PPA4 > 0.5) on chromosomes 1, 6, 7, 9 and 19, implicating pleiotropic genes, including NAV1, IPO9, PHACTR1, UFL1, FHL5, and FOCAD. Conclusions: Current findings reveal limited genetic correlation and no significant causal associations of CAC and AAC with AD or cognitive traits. However, significant pleiotropic loci, particularly at the APOE region, highlight the complex interplay between vascular calcification and neurodegenerative processes. Given APOE’s roles in lipid metabolism, neuroinflammation, and vascular integrity, its involvement may link vascular and neurodegenerative disorders, pointing to potential targets for further investigation. Full article

Background/Objectives: UFMylation, a newly identified ubiquitin-like modification, modulates a variety of physiological processes, including endoplasmic reticulum homeostasis maintenance, DNA damage response, embryonic development, and tumor progression. Recent reports showed that UFMylation plays a protective role in preventing liver steatosis and fibrosis, serving as a defender of liver homeostasis in the development of metabolic dysfunction-associated steatotic liver disease (MASLD). However, the regulation of UFMylation in MASLD remains unclear. This study aimed to determine the expressed patterns of UFMylation components in multiple tissues of leptin-deficient ob/ob mice and high-fat diet (HFD)-fed mice, which are mimicking the conditions of MASLD. Methods: The ob/ob mice and HFD-fed mice were sacrificed to collect tissues indicated in this study. Total RNA and proteins were extracted from tissues to examine the expressed patterns of UFMylation components, including UBA5, UFC1, UFL1, DDRGK1, UFSP1, UFSP2 and UFM1, by real-time PCR and western blot analysis. Results: The protein levels of UBA5, UFC1 and UFL1 were down-regulated in liver, brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT), whereas the messenger RNA (mRNA) levels of Ufl1 and Ufsp1 were both decreased in skeletal muscle, BAT, iWAT and epididymal white adipose tissue (eWAT) of ob/ob mice. In contrast, the mRNA levels of Ufsp1 in skeletal muscle, BAT, iWAT and heart, and the protein levels of UFL1 were decreased in BAT, iWAT, heart and cerebellum of HFD-fed mice. Conclusions: Our findings established the expressed profiles of UFMylaiton in multiple tissues of mice mimicking MASLD, indicating an important regulation for UFMylation in these tissues’ homeostasis maintenance. Full article

Background: Voriconazole is an antifungal drug, which is classified under Bio-Classification System-II and has low water solubility (0.71 mg/mL) and high permeability. Hardly any endeavors have been made to increase the bioavailability of voriconazole. Objective: To develop and evaluate a solid SMEDDS (self-microemulsifying drug delivery system) for antifungal activity. Methods: Based on solubility studies of Labrafil-M 1994 CS (oil), Cremophor-RH 40 (a surfactant) and Transcutol-HP (a co-surfactant) were selected as components of the SMEDDS and a pseudo-ternary phase diagram was prepared. Thereafter, the oil, surfactant, and co-surfactant were mixed with altered weight ratios (1:1/1:2/2:1) and evaluated through various in vitro, in vivo analyses. Results: The particle size of the optimized formulation was observed to be 19.04 nm and the polydispersity index (PDI) value was found to be 0.162 with steady-state zeta potential. The optimized liquid SMEDDS was converted into a solid SMEDDS. Various adsorbents, such as Aerosil-200, Avicel-PH101, Neusilin-US2, and Neusilin UFL2 were screened to better detect the oil-absorbing capacity and flow properties of the powder. Neusilin UFL2 was selected as an adsorbent due to its better oil-absorbing capacity. DSC, X-ray diffraction, and dissolution studies were carried out to characterize the formulation. Further, the Pharmacokinetic profile was also studied in Wistar rats and the Cmax, tmax, and AUC0→t were calculated. The Cmax and AUC0→t plasma concentration is considerably better for the SMEDDS than for the pure drug and marketed formulation. Conclusions: This investigation clearly reveals the potential of developing a solid SMEDDS for candidiasis and invasive aspergillosis treatment, with better efficacy as compared to the commercially available marketed formulation. Full article

The data presented in this paper are related to the paper entitled “Optimal Estimation of Under-Frequency Load Shedding Scheme Parameters by Considering Virtual Inertia Injection”, available in the Energies journal. Here, data are included to show the results of an Under-Frequency Load Shedding (UFLS) scheme that considers the injection of virtual inertia by a VSC-HVDC link. The data obtained in six cases which were considered and analyzed are shown. In this paper, each case represents a different frequency response configuration in the event of generation loss, taking into account the presence or absence of a VSC-HVDC link, traditional and optimized UFLS schemes, as well as the injection of virtual inertia by the VSC-HVDC link. Data for each example contain the state of the relay, threshold, position in every delay, load shed, and relay configuration parameters. Data were obtained through Digsilent Power Factory and Python simulations. The purpose of this dataset is so that other researchers can reproduce the results reported in our paper. Full article

Under-frequency load shedding (UFLS) schemes are the latest safety measures applied for safeguarding the integrity of the grid against abrupt frequency imbalances. The overall inertia of electrical power systems is expected to decrease with an increased penetration of renewable energy as well as elements connected through power electronic interfaces. However, voltage source converter-based high voltage direct current (VSC-HVDC) links can provide virtual inertia through a control loop that allows for a reaction to occur at certain frequency fluctuations. This paper evaluates a UFLS scheme that considers the injection of virtual inertia through a VSC-HVDC link. A genetic algorithm (GA) is used to determine the location of the UFLS relays, the activation threshold of each stage, the delay time and the percentage of load shedding at each stage. It was found that the virtual inertia causes the nadir to delay and sometimes reach a greater depth. Furthermore, the implemented GA approximates the frequency response to the limits set with the constraints, reducing the load shedding but achieving a steeper nadir and a lower steady-state frequency level than traditional UFLS. The simulations were performed using the IEEE 39-bus test system. Full article

With the high penetration of renewable energy into power grids, frequency stability and oscillation have become big concerns due to the reduced system inertia. The application of the Battery Energy Storage System (BESS) is considered one of the options to deal with frequency stability and oscillation. This paper presents a strategy to size, locate, and operate the BESS within the power grid and, therefore, investigate how sizing capacity is related to renewable energy penetration levels. This paper proposes an identification method to determine the best location of the BESS using the Prony method based on system oscillation analysis, which is easy to implement based on measurements while actual physical system models are not required. The proposed methods for BESS size and location are applied using MATLAB/Simulink simulation software (version: R2023a) on the Kundur 2-area 11-bus test system with different renewable energy penetration levels, and the effectiveness of the applied method in enhancing frequency stability is illustrated in the study cases. The case studies showed a significant improvement in steady-state frequency deviation, frequency nadir, and Rate of Change of Frequency (ROCOF) after implementing BESS at the selected bus. The integration of BESS can help to avoid Under-frequency Load Shedding (UFLS) by proper selections of size, location, and operating strategy of the BESS within the power grid. Full article

An Electric Power System (EPS) is a dynamic system that, due to continuous variations in the load, the presence of disturbances, switching operations, and/or the operation of the protection system, is never in a steady state. A deficit in generation causes a drop in the system’s frequency that, if not controlled, could result in the loss of synchronism between generators or areas and, in the worst-case scenario, a total or partial system collapse. This article presents a methodology to identify a subset of representative events that generalizes the N-1 generation contingency space; this subset can later be applied in the development of Under-Frequency Load-Shedding (UFLS) schemes based on the Rate of Change of Frequency (ROCOF). Full article

This study proposes an under-frequency load-shedding (UFLS) scheme based on a binary Archimedes Optimization Algorithm (BAOA) and the Weighted Sum Method (WSM) to maintain the stability of an islanded distribution system. These methods consider stability indices and load priorities to ensure effective load shedding during frequency deviations. The BAOA determines the optimal load shedding based on the stability index and power mismatch that minimizes the impact on critical loads while maintaining system stability in an islanded distribution system. The WSM determines the rank of the load to be shed based on four criteria: the load priority, the load category, the stability index, and the load size. Each load is assigned a weight based on its priority. These weight variables determine the order in which loads are shed during frequency deviations. The effectiveness of the proposed UFLS was tested on an 11 kV Malaysian distribution network with two mini hydro distributed generation systems. A comparative study was conducted based on five result outputs, including the number of loads shed, the size of the loads shed, the frequency undershoot, the frequency overshoot, and the time taken to achieve a stable frequency in three cases: base load, peak load, and peak load with photovoltaics (PV). The proposed UFLS showed the best results for 11 of 15 outputs (73.3%) for islanding events and 9 of 15 outputs (60%) for overloading events. The voltage profile and stability index, also, were improved after the proposed UFLS was applied. Full article

In a constantly and rapidly changing global environment, one of the main priority tasks for every country is preserving, maintaining, and operating an independent and individually robust and stable energy system. This paper aims at researching electrical power systems’ (EPSs) behavior during desynchronization from a synchronous area, its stability in islanded mode, and its synchronization. The analysis of EPS behavior was accomplished utilizing numerical simulations in a widely used programming/simulation package. The sudden tripping of the EPS into an isolated island mode with known generation and load values was simulated, analyzed, and discussed. We investigated the behavior of an isolated EPS in the case of the loss of a certain amount of active power, and determined the maximum power that must be available to ensure the reliable operation of the isolated EPS and the power reserve that must be maintained to prevent the EPS from triggering UFLS. The simulation of the synchronization of an isolated EPS with a synchronous area was accomplished and analyzed. The obtained results were applied to reveal the sequence of actions that will help an EPS to ensure and maintain the stable and reliable operation of electrical installations during desynchronization. Full article

Appropriate frequency response is an issue of great importance in power system management, especially in an islanded one. An energy-based method for assessing a system’s response, which is needed to prevent under frequency load shedding (UFLS), is introduced. Renewable generation, such as wind turbine (WT) and photovoltaic (PV) facilities, reduces the ability of the power system to resist power imbalances and increases the risks of consumer disconnections by UFLS system, and even of total collapse. To estimate the amount of additional fast power reserve, an equation was developed, relating the moment of inertia, the system demand dynamics, and the available response of synchronous generating units. Clustering units based on their ability to respond to frequency changes in low inertia conditions allows the potential synchronous response to be assessed, providing information of its deficiency in a defined system state. The proposed method was applied to the Israeli power system and up to 307 MW response needed from PV facilities was found for the 350 MW contingency, when the percentage of renewable energy reached 30% of the annual energy production. This study focused on proportional frequency response (PFR) and step frequency response (SFR) that PV facilities can provide. Using this method may contribute to the adoption of PV facilities into the power system without a detrimental impact on frequency response and may even improve the reliability of electricity supply. Full article

The direct tailoring of the size, composition, or number of layers belongs to the advantages of 3D printing employment in producing orodispersible films (ODFs) compared to the frequently utilized solvent casting method. This study aimed to produce porous ODFs as a substrate for medicated ink deposited by a 2D printer. The innovative semi-solid extrusion 3D printing method was employed to produce multilayered ODFs, where the bottom layer assures the mechanical properties. In contrast, the top layer provides a porous structure for ink entrapment. Hydroxypropyl methylcellulose and polyvinyl alcohol were utilized as film-forming polymers, glycerol as a plasticizer, and sodium starch glycolate as a disintegrant in the bottom matrix. Several porogen agents (Aeroperl^® 300, Fujisil^®, Syloid^® 244 FP, Syloid^® XDP 3050, Neusilin^® S2, Neusilin^® US2, and Neusilin^® UFL2) acted as porosity enhancers in the two types of top layer. ODFs with satisfactory disintegration time were prepared. The correlation between the porogen content and the mechanical properties was proved. A porous ODF structure was detected in most samples and linked to the porogen content. SSE 3D printing represents a promising preparation method for the production of porous ODFs as substrates for subsequent drug deposition by 2D printing, avoiding the difficulties arising in casting or printing medicated ODFs directly. Full article

The conceptual description of Follicular lymphoma (FL) in the 5th edition of the World Health Organization (WHO) classification of haematolymphoid tumors (WHO-HAEM5) has undergone significant revision. The vast majority of FL (85%) with a follicular growth pattern are composed of centrocytes and centroblasts, harbor the t(14;18)(q32;q21) translocation and are now termed classic FL (cFL). They are set apart from three related subtypes, FL with predominantly follicular growth pattern, FL with unusual cytological features (uFL) and follicular large B-cell lymphoma (FLBCL). In contrast to the revised 4th edition of the WHO classification of haematolymphoid tumors (WHO-HAEM4R), grading of cFL is no longer mandatory. FL with a predominantly diffuse growth pattern had been previously recognized in WHO-HAEM4R. It frequently occurs as a large tumor in the inguinal region and is associated with CD23 expression. An absence of the IGH::BCL2 fusion and frequent STAT6 mutations along with 1p36 deletion or TNFRSF14 mutation is typical. The newly introduced subtype of uFL includes two subsets that significantly diverge from cFL: one with “blastoid” and one with “large centrocyte” variant cytological features. uFL more frequently displays variant immunophenotypic and genotypic features. FLBCL is largely identical to WHO-HAEM4R FL grade 3B and renaming was done for reasons of consistency throughout the classification. In-situ follicular B-cell neoplasm, pediatric-type FL, duodenal-type FL and primary cutaneous follicle center lymphoma are categorized as discrete entities. In addition, novel findings concerning underlying biological mechanisms in the pathogenesis of early and systemic follicular lymphoma will be presented. Full article