Search Results (902)

Mercury (Hg) decontamination in active and decommissioned mining areas is a difficult task since Hg may affect environmental matrices and man-made materials. Despite its toxicity as an inorganic form being rather limited with respect to organic compounds (e.g., methyl-Hg), severe effects to human health and ecosystems are recognized. In this work, we review the geochemical activities carried out in the last 13 years at the Abbadia San Salvatore (AbSS) mining and production area. This site belongs to Mt. Amiata (Tuscany, central Italy), which is considered the third-largest Hg-district in the world. Air, water, soil and man-made materials within the AbSS area were investigated to verify to what extent such matrices were affected by Hg contamination. The geochemical investigations are used as important tools to proceed with specific remediation operations of edifices, mining structures and machineries as well as the local groundwater system. To the best of our knowledge, restoration of decommissioned areas affected by Hg contamination at a large scale, such as the AbSS exploitation and production site, is rather uncommon. Currently, the remediation activities in the AbSS area are going on and they are expected to be concluded at the end of 2026 or the beginning of 2027, when the former mining area will turn into a public archeometallurgical museum. Full article

Old-growth forests play a vital role in the conservation of terrestrial biodiversity, though they are rare and increasingly threatened worldwide. The Mediterranean region hosts notable examples of these ecosystems, but information about their location, structure, and biodiversity is still largely incomplete. In this work, we tested the hypothesis that the region of Tuscany (Italy) harbors forest sites with old-growth characteristics in light of the EU indicators and the Italian ministerial guidelines. Accordingly, data on stand structural and plant diversity variables were collected in 27 plots located in pre-selected sites across different forest types of the region. As a result, 12 sites were inventoried that can be proposed as candidates for the national network of old-growth forests. These were largely unknown, ca. 10–300 ha in surface and encompassing five main forest types across 14 Natura2000 habitats. All stands have reached the mature or nearly senescent stage thanks to natural dynamic processes for over 70 years after the cessation of substantial anthropogenic disturbances. The structural heterogeneity index (SHI), based on living and deadwood biomass variables, was relatively high (66.2–84%). However, structural variables depended on forest type, thus on bioclimatic context and dominant tree species. Stands with beech and mountain conifers showed more pronounced old-growth characteristics than Mediterranean stands due to a faster recovery dynamic after cessation of disturbance. As many as 193 vascular plant taxa were recorded, with 16 species occurring with trees ≥ 50 cm in diameter. Forest specialist taxa, either woody or herbaceous, were prevalent, but numerous generalists also occurred in the gaps. Ancient forest species were also well represented, supporting the long temporal continuity of the forests. This work advances knowledge about forest sites with old-growth characteristics in southern Europe, contributing to the implementation of the national network and the EU Biodiversity Strategy 2030. Strict protection of these sites is necessary to allow the forest stands to fully reach the old-growth stage in the next decades, despite the negative influence of climate change. Full article

This study evaluated contamination by polychlorinated dibenzodioxins and dibenzofurans (PCDDs/Fs) and polychlorinated biphenyls (PCBs) in 390 feeds and 1756 food samples collected in Latium and Tuscany (Italy, 2016–2025) using HRGC-HRMS. PCDDs/Fs and dioxin-like PCBs (dl-PCBs) are expressed as WHO 2005 toxic equivalents (WHO₀₅-TEQ). Non-dioxin-like PCBs (ndl-PCBs) lack dioxin-like toxicity mechanisms due to their non-coplanar structure and are not assigned a toxic equivalence factor. Feed results were normalised to 12% moisture content. Median levels of WHO₀₅-PCDDs/Fs+dl-PCBs TEQ at the upper limit in feed were 10–100 times lower than those reported in European monitoring data (EFSA, 2002–2010) for comparable categories, including additives, premixtures, raw materials and compound feed, with plant and animal feed materials below 0.03 ng/kg and aquaculture feed at 0.24 ng/kg. Food contamination was generally low, with the median WHO₀₅-PCDDs/Fs+dl-PCBs TEQ 2–4 times lower than Italian national data (2013–2016), considering comparable categories such as meat, fish, milk, eggs, oils, baby foods, marine oils, animal fats and liver. Higher levels were observed in game meat, sheep products and fermented milk than in pork and poultry. The contamination remained stable over time. These results indicate an improvement in food safety thanks to national and EU regulations, although continued surveillance of high-risk and undersampled categories remains essential. Full article

This review provides a comprehensive overview of the most adopted 3D surveying techniques in Cultural Heritage, offering practical guidance for the selection of appropriate methods when three-dimensional documentation of artworks is required. The analysis focuses on the most effective technologies for the 3D documentation of sites and objects of artistic value, with selection criteria primarily centred on non-invasiveness, given the uniqueness and cultural significance of the case studies, and the instrument flexibility, a crucial requirement for non-transportable items. A broad spectrum of 3D techniques is currently available for the multiscale diagnostic investigation of artworks, providing information at both macroscopic and microscopic levels. This review reports on the state of the art of such systems and evaluates the main characteristics of each technology in relation to its applicability in the heritage field. Particular attention is given to highlighting advantages and limitations, and to assessing performance in terms of resolution, gauge volume/area, acquisition time, and cost. In addition, the review discusses exemplary cases in which 3D methods are integrated with other analytical techniques to enable a more comprehensive understanding of the object under investigation. Finally, recent studies are examined to identify the most suitable approaches and the specific requirements for the digitization of real-world heritage assets. Full article

Recent Italian developments in end-of-life governance have intensified debate on self-determination, medically assisted suicide, and the constitutional limits of healthcare regulation. This article is a narrative review combined with doctrinal legal analysis and medico-legal commentary. It examines Tuscany’s Regional Law No. 16 of 14 March 2025 within the broader Italian framework shaped by Law No. 219/2017, Constitutional Court Judgment No. 242/2019, and the subsequent constitutional review culminating in Judgment No. 204/2025. The article pursues three aims: to reconstruct the national legal framework governing end-of-life decision-making in Italy; to analyse the structure and constitutional implications of the Tuscan statute; and to assess the medico-legal relevance of the persistent uncertainty surrounding life-sustaining treatments as an eligibility criterion. The analysis highlights two distinct but interconnected issues: the constitutional boundary between regional healthcare organisation and matters requiring nationally uniform safeguards, and the unresolved interpretation of life-sustaining treatments in clinical and legal practice. In light of Judgment No. 204/2025, the article argues that regional procedural intervention may reduce administrative uncertainty, but cannot replace coherent parliamentary legislation capable of clarifying substantive criteria, limiting territorial variability, and reinforcing the role of palliative care within end-of-life pathways. Full article

Ips typographus is one of the most destructive bark beetles affecting conifer forests in Europe, where climatic disturbances and the movement of infested wood can rapidly shift populations from endemic levels to severe outbreaks. Early detection through border inspections and forest monitoring is essential to prevent new introductions and limit the spread of established populations. Here, we developed and validated a probe-based TaqMan qPCR assay, targeting the mitochondrial COI barcode region, for the rapid and species-specific detection of I. typographus from both insect material and environmental DNA recovered from frass and exit-hole wood chips. Validation followed EPPO PM7/98(5) guidelines, assessing analytical specificity, sensitivity, repeatability, reproducibility, and inter-laboratory transferability. High analytical specificity was demonstrated against a broad panel of non-target species, and reliable amplification was obtained across different tested matrices. The method showed strong analytical sensitivity, with limits of detection of 0.32 pg/µL for adult-derived DNA and 1.6 pg/µL for artificial frass. Repeatability, reproducibility, and inter-laboratory blind testing further confirmed the diagnostic reliability of the method. This validated qPCR assay provides a rapid and sensitive molecular tool for the early detection of I. typographus, supporting border inspection and phytosanitary diagnostic laboratories in forest biosecurity activities. Full article

The reuse of treated wastewater for agricultural irrigation is increasingly considered a strategic response to water scarcity and climate change, particularly in Mediterranean regions. This study examines the local feasibility and social acceptance of water reuse within the framework of Regulation (EU) 2020/741, focusing on its implementation in Italy. The research combines policy analysis, technical assessment of effluent quality from the GIDA wastewater treatment plant (Prato, Tuscany), GIS-based spatial evaluation, and a mixed-method survey of local agri-food producers. Results show substantial compliance with EU minimum quality requirements, alongside additional constraints arising from national regulatory thresholds. Survey findings reveal cautious but tangible openness among farmers toward reclaimed water use, particularly in response to increasing climate-related pressures. The case of Prato is further analysed within the Prato Circular City and local food policy frameworks, highlighting the role of participatory governance and multi-actor engagement in supporting reuse initiatives. The study contributes empirical evidence on the interaction between EU regulation, national implementation measures, and local socio-institutional conditions shaping peri-urban water reuse systems. Furthermore, it serves as a preliminary framework for future economic feasibility studies and the subsequent regulatory and permitting phases required to operationalize this practice. Full article

Toscana virus (TOSV) is an arthropod-borne virus, transmitted by sandflies, which represents a major cause of aseptic meningitis in Mediterranean countries during summer months. Despite its epidemiological importance, recent genomic data on circulating Italian strains remain limited. We performed comprehensive phylogenetic and genotypic characterization of 34 TOSV isolates (32 obtained from human biological samples and 2 from sandfly homogenates) collected between 2022 and 2025 from Northern/Central Italy. All the sequenced isolates clustered within Lineage A, with strains circulating in Tuscany showing significantly lower intra group genetic divergence (p < 0.05), indicative of compartmentalized local circulation. Both S and M segments exhibited negative selection and identified non-synonymous mutations deserving functional investigation. This study documents stable Lineage A predominance across Italian regions, with Tuscany showing distinct phylogeographic structuring. mNGS proves effective for TOSV genomic surveillance, supporting refined public health strategies, including targeted sandfly control in endemic foci. Full article

Background and Objectives: Children with specific learning disorders (SLD) often present a history of speech and language deficits. However, systematic evidence on the co-occurrence among distinct learning and communication disorders remains limited. This study aimed to describe the associations among reading, spelling, and math disorders and their relationships with clinically diagnosed speech sound and language disorders and speech sound disorders in a large, well-characterized clinical sample. Methods: 235 3rd- to 8th-grade Italian children with SLD participated in the study. They were categorized in terms of learning (reading, spelling, and math) and comorbid communication disorders (speech sound, and language disorders), according to established diagnostic criteria. Prevalence rates were assessed for each of the resulting subgroups. Results: Comorbidity between the three learning disorders was very frequent; 75.4% of children showed different forms of multiple SLDs, with 47.7% presenting a combined reading, spelling, and math disorder. Communication disorders were reported in 40.4% of the sample. Both language and speech sound disorders frequently co-occurred with spelling disorders, whereas associations with isolated reading or math disorders were more infrequent. Additionally, speech sound disorders frequently co-occurred with isolated spelling disorders, whereas language disorders frequently co-occurred with comorbid spelling disorders. Conclusions: Consistent with previous evidence, the study shows that learning disorders are highly comorbid with communication disorders. Critically, speech and language disorders are most frequently comorbid with spelling disorder, independent of reading and math deficits, highlighting spelling as a potential key interface between phonology, language, and learning. Full article

Pertussis, caused by Bordetella pertussis, remains a public health concern despite long-standing vaccination programs. After a marked decline during the COVID-19 pandemic, a resurgence was observed in Europe and Italy, with a sharp increase in 2024. This study describes pertussis epidemiological trends in the Tuscany Region (Italy) from 2019 to 2024 to identify high-risk groups and inform prevention strategies. A retrospective population-based analysis was conducted using cases reported to the national surveillance system (PREMAL). Incidence rates were calculated using ISTAT population data, and demographic, temporal, and clinical characteristics were analyzed. Overall, 669 cases were reported (mean annual incidence rate: 3.03/100,000 (IC 95% 2.47–3.59; period incidence rate: 18.2/100,000 (IC 95% 16.81–19.56)), with 89% occurring in 2024 (16.34/100,000 (IC 95% 15.03–17.65)). No sex differences were observed, and most cases were reported in Central Tuscany (64%). Children under 15 years accounted for 87% of cases. The highest incidence was observed among 10–14-year-olds, while infants < 1 year, particularly those under 4 months, showed the highest burden in narrower age strata. Hospitalizations occurred in 12.6% of cases, decreasing substantially in 2024. The 2024 resurgence likely reflects waning immunity, disruptions to routine vaccinations during the pandemic, and reduced pathogen circulation in previous years due to containment and isolation measures related to the pandemic. Strengthening surveillance and improving booster and maternal vaccination coverage are essential to protect vulnerable populations. Full article

Background/Objectives: Among the antiretroviral therapies (ARTs) recently introduced for people living with HIV (PLWH), the fixed-dose combination of bictegravir, emtricitabine and tenofovir alafenamide (B/F/TAF) became reimbursable in Italy in June 2019. Methods: This study evaluated drug utilization, healthcare resource consumption and direct costs among ART-naïve adults initiating B/F/TAF or other non-bictegravir-based regimens, identified from June 2019 to September 2022 within administrative databases of healthcare entities covering approximately nine million citizens. Baseline clinical characteristics at first ART prescription were compared across B/F/TAF-treated patients, those receiving other ART regimens, and non-HIV controls, while treatment outcomes during follow-up were evaluated among PLWH receiving B/F/TAF or other ARTs; healthcare consumption and costs were assessed after propensity score matching within the PLWH cohorts only. Results: Overall, 374 individuals initiated B/F/TAF and 5576 other ARTs. Patients treated with B/F/TAF showed greater adherence and persistence, with multivariate analyses confirming a lower risk of discontinuation or switching (HR = 0.66, 95% CI 0.57–0.76, p < 0.001) and a higher likelihood of adherence (HR = 2.40, 95% CI 1.58–3.64, p < 0.001). After matching, the B/F/TAF group exhibited lower 12-month consumption of non-HIV medications, fewer non-HIV hospitalizations, and reduced total healthcare costs, particularly for non-HIV drug prescriptions compared to other ART users. Conclusions: Overall, B/F/TAF was associated with better treatment continuity and meaningful cost savings. Full article

Accurate urban noise mapping requires granular traffic flow characterization aligned with specific acoustic models, such as CNOSSOS-EU. Existing monitoring solutions often lack the specific categorization capabilities, cost-effectiveness, or flexibility required for large-scale deployment in resource-constrained environments. To address this challenge, the present study describes the development of a real-time multi-vehicle recognition system based on low-cost edge computing hardware, specifically a Raspberry Pi 4 coupled with a Coral TPU accelerator. The proposed methodology integrates a quantized YOLOv8 convolutional neural network (CNN) with a tracking algorithm to enable real-time detection and classification of vehicles into five distinct classes, allowing for precise aggregation according to CNOSSOS-EU standards. The model was trained on a proprietary dataset of 15,000 images and subjected to 8-bit post-training quantization to optimize inference speed. Experimental results demonstrate that the system achieves an inference speed of 14 FPS and a mean Average Precision (mAP@50) of 92.2% in daytime conditions, maintaining robust performance on embedded devices. In a real-world case study, the proposed system significantly outperformed a commercial traffic monitoring solution, achieving a weighted percentage error of just 6.6% compared to the commercial system’s 59.9%, effectively bridging the gap between manual counting accuracy (1.4% error) and automated efficiency. Full article

Background: Postoperative wounds may arise from several etiologies, including open partial pedal amputation, postoperative infection, and dehiscence of surgical sites from wound failure or patient compliance issues. If negative pressure wound therapy is the gold standard, its application in the toes area could be challenging, and as a consequence, standard care is most likely used. The control of the wound microenvironment, both in terms of pH levels and presence of reactive oxygen species, is a key part of the normal wound-healing process. This study evaluated the effectiveness of an oxygen-enriched oil-based device (OEOd) in post-surgical diabetic foot ulcers (DFUs). Methods: This prospective controlled comparative pilot study enrolled 40 patients with diabetes mellitus and post-surgical foot wounds (narrow and deep lesions, including tunneling ulcers) treated at the Diabetic Foot Unit of San Donato Hospital, Arezzo (March 2024–April 2025). Patients were allocated into two groups: those treated by the standard wound care (n = 20) and those treated by OEOd (n = 20). The primary outcome was complete wound healing at 16 weeks; other exploratory endpoints were wound area reduction at 4 and 16 weeks, onset of infection, need for re-intervention, and adverse events. Results: Complete wound healing was achieved in 85.0% of OEOd patients versus 45.0% in the control group (p = 0.020). At 16 weeks, wound area reduction was significantly greater in the OEOd group compared with standard therapy (89.8% vs. 64.0%, p = 0.013). Although infection rates (10.0% vs. 35.0%, p = 0.130) and need for re-intervention (0% vs. 25.0%, p = 0.056) did not reach statistical significance, both favored the OEOd group. No adverse events were reported. Conclusions: OEOd significantly improved the chance of healing post-surgery and showed favorable trends in reducing complications, with an excellent safety profile. Larger randomized controlled trials are warranted to confirm these findings and assess long-term outcomes. Full article

Soil erosion assessments for policy are often derived from continental-scale datasets, but their suitability for regional planning remains unclear. This study compares two Revised Universal Soil Loss Equation (RUSLE) applications for Tuscany, Italy: one using high-resolution regional data (TuscReg) and another using European-scale data from the European Soil Data Centre (TuscNat). We found the mean estimated actual soil erosion rate was 58% higher in the regional assessment (10.7 vs. 6.8 Mg ha⁻¹ yr⁻¹). Remarkably, the spatial patterns diverged significantly in the complex landscapes characterizing some Tuscan soil regions. In mountainous areas like the Apuan Alps, TuscReg estimated soil erosion control (potential minus actual erosion) to be over 500 Mg ha⁻¹ yr⁻¹ greater than TuscNat for 30% of the area. Correlation analysis revealed these major differences were primarily driven by disparities in the rainfall erosivity (R) and soil erodibility (K) factors. Our results demonstrate that while EU-scale models provide a consistent, broad-scale overview, they can substantially underestimate erosion and the ecosystem service of erosion control in specific, high-risk environments. To implement policies like the EU Soil Monitoring Law (Directive (EU) 2025/2360), regional-scale data are essential to accurately identify priority areas for soil conservation and set meaningful local thresholds. Full article

The transition towards circular economy is now a key strategy to address the environmental issues we are facing. Within this framework, biochar, a carbon-rich material derived from residual agricultural pyrolysis, can represent a sustainable and circular solution. This paper aims at evaluating the possibility of implementing a local biochar-production system as part of an economic and social strategy of the redevelopment of an abandoned rural site, Borgo di Perolla, in Tuscany, Italy. A cost–benefits analysis (CBA) was conducted to evaluate the economic feasibility of three different scenarios of production and strategies: Scenario 1 considers revenues solely from the production and sale of biochar and wood vinegar; Scenario 2 additionally includes potential income from the sale of voluntary carbon credits; and Scenario 3 incorporates biochar credits within the European Union Emission Trading System (EU ETS). For each scenario, three indicators were calculated: Net-Present Value (NPV), Internal Rate of Return (IRR), and Breakeven point (BEP). The most evident result that emerged is that the sale of biochar and its by-products alone is not sufficient to ensure the project’s economic sustainability, mainly due to high production costs. Only through carbon-credit-trading markets biochar becomes not only an environmentally strategic tool but also an economically rewarding one. In this sense, market infrastructures, such as the ETS, are essential for the dissemination of circular models, like biochar, that generate both environmental and economic benefits. Previous studies on biochar have largely focused on its application and associated benefits, while cost–benefit analyses have primarily examined its economic feasibility through the commercialization of biochar as a soil amendment, particularly within the United States context. The present work contributes to this literature in three main ways. First, it provides a site-specific and replicable CBA framework applied to a real territorial regeneration project (Borgo di Perolla), grounded in primary data collected through field surveys, stakeholder interviews, and expert validation. Second, the study explicitly compares multiple market-access scenarios within the same analytical framework, ranging from biochar-only sales to voluntary carbon markets, allowing for a clear identification of the economic thresholds at which biochar becomes financially sustainable. Third, and most importantly, the main contribution of this work lies in the explicit modeling of biochar integration into the EU Emissions Trading System. This paper extends the analysis to a regulated carbon market scenario, assuming the recognition of biochar-based carbon removals within the EU ETS framework. From a methodological perspective, the study quantitatively assesses how ETS price dynamics affect the profitability, internal rate of return, and break-even point of a biochar project over a long-term horizon. From a policy perspective, the analysis anticipates recent regulatory developments, such as the EU Regulation 2024/3012, on establishing a Union certification framework for permanent carbon removals, carbon farming, and carbon storage in products, by showing how biochar could function as a fully market-integrated climate technology. Full article