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Search Results (922)

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Keywords = Total Farm Management

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18 pages, 1486 KiB  
Article
Occurrence and Reasons for On-Farm Emergency Slaughter (OFES) in Northern Italian Cattle
by Francesca Fusi, Camilla Allegri, Alessandra Gregori, Claudio Monaci, Sara Gabriele, Tiziano Bernardo, Valentina Lorenzi, Claudia Romeo, Federico Scali, Lucia Scuri, Giorgio Bontempi, Maria Nobile, Luigi Bertocchi, Giovanni Loris Alborali, Adriana Ianieri and Sergio Ghidini
Animals 2025, 15(15), 2239; https://doi.org/10.3390/ani15152239 - 30 Jul 2025
Abstract
On-farm emergency slaughter (OFES) is employed when cattle are unfit for transport but still suitable for human consumption, thereby ensuring animal welfare and reducing food waste. This study analysed OFES patterns in Northern Italy, where a large cattle population is housed but information [...] Read more.
On-farm emergency slaughter (OFES) is employed when cattle are unfit for transport but still suitable for human consumption, thereby ensuring animal welfare and reducing food waste. This study analysed OFES patterns in Northern Italy, where a large cattle population is housed but information on the practice is rarely analysed. A total of 12,052 OFES cases from 2021 to 2023 were analysed. Most involved female cattle (94%) from dairy farms (79%). Locomotor disorders were the leading reason (70%), particularly trauma and fractures, followed by recumbency (13%) and calving-related issues (10%). Post-mortem findings showed limbs and joints as the most frequent condemnation sites (36%), often linked to trauma. A significant reduction in OFES cases occurred over time, mainly due to fewer recumbency and calving issues, likely reflecting stricter eligibility criteria introduced in 2022. Weekly variations, with peaks on Mondays and lows on Saturdays, suggest that logistical constraints may sometimes influence OFES promptness. These findings suggest that on-farm management and animal handling could be improved further to reduce welfare risks and carcass waste. Due to the lack of standardised data collection and regulatory harmonisation, a multi-country investigation could improve our understanding of this topic and inform best practice. Full article
(This article belongs to the Special Issue Ruminant Welfare Assessment—Second Edition)
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13 pages, 239 KiB  
Article
In Vitro Detection of Acaricide Resistance in Hyalomma Species Ticks with Emphasis on Farm Management Practices Associated with Acaricide Resistance in Abu Dhabi, United Arab Emirates
by Shameem Habeeba, Yasser Mahmmod, Hany Mohammed, Hashel Amer, Mohamed Moustafa, Assem Sobhi, Mohamed El-Sokary, Mahmoud Hussein, Ameer Tolba, Zulaikha Al Hammadi, Mohd Al Breiki and Asma Mohamed Shah
Vet. Sci. 2025, 12(8), 712; https://doi.org/10.3390/vetsci12080712 - 29 Jul 2025
Viewed by 189
Abstract
Acaricide usage has led to the growing problem of resistance in ticks. A heavy tick burden and the presence of ticks on animals throughout the year, despite the monthly application of acaricides, in farms in the United Arab Emirates formed the motivation for [...] Read more.
Acaricide usage has led to the growing problem of resistance in ticks. A heavy tick burden and the presence of ticks on animals throughout the year, despite the monthly application of acaricides, in farms in the United Arab Emirates formed the motivation for this study. The objectives of this research were as follows: (a) to assess the acaricide resistance status of the most prevalent tick Hyalomma spp. to widely used acaricides Cypermethrin and Deltamethrin; (b) to identify the association of farm management practices and farm-level risk factors with the failure of tick treatment (acaracide resistance). A total of 1600 ticks were collected from 20 farms located in three different regions of Abu Dhabi Emirate including Al Ain (n = 10), Al Dhafra (n = 5), and Abu Dhabi (n = 5). The ticks were subjected to an in vitro bioassay adult immersion test (AIT) modified with a discriminating dose (AIT-DD) against commercial preparations of Cypermethrin and Deltamethrin. A questionnaire was designed to collect metadata and information on farm management and the farm-level risk factors associated with routine farm practices relating to the treatment and control of tick and blood parasite infections in camels and small ruminant populations. Hyalomma anatolicum and Hyalomma dromedarii were identified among the collected ticks, with H. anatolicum being the most prevalent tick species (70%) in the present study. The test results of the in vitro bioassay revealed varied emerging resistance to both of the acaricides in the majority of the three regions; fully susceptible tick isolates with zero resistance to Deltamethrin were recorded in one farm at Al Ain and two farms in the Abu Dhabi region. A questionnaire analysis showed that the failure of tick treatment in farms varied with the presence or absence of vegetation areas, types of animal breeds, and management practices. This study reports the emergence of resistance in ticks to Cypermethrin and Deltamethrin across the Abu Dhabi Emirate, indicating a strict warning for the cautious use of acaricides. There is also a need to improve awareness about sound tick management and control practices among farm owners through a multidisciplinary approach adopting integrated pest management strategies that engage farmers, veterinarians, and policy makers. Full article
(This article belongs to the Topic Ticks and Tick-Borne Pathogens)
17 pages, 1397 KiB  
Article
Comparison of Soil Organic Carbon Measurement Methods
by Wing K. P. Ng, Pete J. Maxfield, Adrian P. Crew, Dayane L. Teixeira, Tim Bevan and Matt J. Bell
Agronomy 2025, 15(8), 1826; https://doi.org/10.3390/agronomy15081826 - 28 Jul 2025
Viewed by 130
Abstract
To enhance agricultural soil health and soil organic carbon (SOC) sequestration, it is important to accurately measure SOC. The aim of this study was to compare common methods for measuring SOC in soils in order to determine the most effective approach among different [...] Read more.
To enhance agricultural soil health and soil organic carbon (SOC) sequestration, it is important to accurately measure SOC. The aim of this study was to compare common methods for measuring SOC in soils in order to determine the most effective approach among different agricultural land types. The measurement methods of loss-on-ignition (LOI), automated dry combustion (Dumas), and real-time near-infrared spectroscopy (NIRS) were compared. A total of 95 soil core samples, ranging in clay and calcareous content, were collected across a range of agricultural land types from forty-eight fields across five farms in the Southwest of England. There were similar and positive correlations between all three methods for measuring SOC (ranging from r = 0.549 to 0.579; all p < 0.001). On average, permanent grass fields had higher SOC content (6.6%) than arable and temporary ley fields (4.6% and 4.5%, respectively), with the difference of 2% indicating a higher carbon storage potential in permanent grassland fields. Newly predicted conversion equations of linear regression were developed among the three measurement methods according to all the fields and land types. The correlation of the conversation equations among the three methods in permanent grass fields was strong and significant compared to those in both arable and temporary ley fields. The analysed results could help understand soil carbon management and maximise sequestration. Moreover, the approach of using real-time NIRS analysis with a rechargeable portable NIRS soil device can offer a convenient and cost-saving alternative for monitoring preliminary SOC changes timely on or offsite without personnel risks from the high-temperature furnace and chemical reagent adopted in the LOI and Dumas processes, respectively, at the laboratory. Therefore, the study suggests that faster, lower-cost, and safer methods like NIRS for analysing initial SOC measurements are now available to provide similar SOC results as traditional soil analysis methods of the LOI and Dumas. Further studies on assessing SOC levels in different farm locations, land, and soil types across seasons using NIRS will improve benchmarked SOC data for farm stakeholders in making evidence-informed agricultural practices. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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32 pages, 1770 KiB  
Article
Regional Patterns in Weed Composition of Maize Fields in Eastern Hungary: The Balance of Environmental and Agricultural Factors
by Mihály Zalai, Erzsébet Tóth, János György Nagy and Zita Dorner
Agronomy 2025, 15(8), 1814; https://doi.org/10.3390/agronomy15081814 - 26 Jul 2025
Viewed by 384
Abstract
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to [...] Read more.
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to weed infestations, particularly before the application of spring herbicide treatments. Field investigations were conducted from 2018 to 2021 across selected maize-growing regions in Hungary. Over the four-year period, a total of 51 weed species were recorded, with Echinochloa crus-galli, Chenopodium album, Portulaca oleracea, and Hibiscus trionum emerging as the most prevalent taxa. Collectively, these four species accounted for more than half (52%) of the total weed cover. Altogether, the 20 most dominant species contributed 95% of the overall weed coverage. The analysis revealed that weed cover, species richness, and weed diversity were significantly affected by soil properties, nutrient levels, geographic location, and tillage systems. The results confirm that the composition of weed species was influenced by several environmental and management-related factors, including soil parameters, geographical location, annual precipitation, tillage method, and fertilizer application. Environmental factors collectively explained a slightly higher proportion of the variance (13.37%) than farming factors (12.66%) at a 90% significance level. Seasonal dynamics and crop rotation history also played a notable role in species distribution. Nutrient inputs, particularly nitrogen, phosphorus, and potassium, influenced both species diversity and floristic composition. Deep tillage practices favored the proliferation of perennial species, whereas shallow cultivation tended to promote annual weeds. Overall, the composition of weed vegetation proved to be a valuable indicator of site-specific soil conditions and agricultural practices. These findings underscore the need to tailor weed management strategies to local environmental and soil contexts for sustainable crop production. Full article
(This article belongs to the Special Issue State-of-the-Art Research on Weed Populations and Community Dynamics)
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12 pages, 248 KiB  
Article
Effectiveness of Targeted Advisory Interventions in Enhancing Welfare on Dairy Farms
by Susy Creatini, Cristina Roncoroni, Federica Salari, Iolanda Altomonte, Giovanni Brajon and Mina Martini
Animals 2025, 15(15), 2197; https://doi.org/10.3390/ani15152197 - 25 Jul 2025
Viewed by 102
Abstract
Animal welfare assessments have raised farmers’ awareness of their management practices, contributing to measurable improvements. However, these protocols often highlight critical points without providing clear guidance on the prioritization of corrective actions. To address this gap, qualified advisory support may play a pivotal [...] Read more.
Animal welfare assessments have raised farmers’ awareness of their management practices, contributing to measurable improvements. However, these protocols often highlight critical points without providing clear guidance on the prioritization of corrective actions. To address this gap, qualified advisory support may play a pivotal role in translating assessments into effective and sustainable interventions. This study evaluates the impact of direct and continuous expert support on improving animal welfare in dairy farms. Data were collected from 21 dairy farms in southeastern Tuscany (Italy) using the Classyfarm (CReNBA) protocol. Each farm underwent two assessments at a three-month interval (T0 and T1), during which tailored support was provided to address specific criticalities. At T0, over 60% of the farms obtained only marginally acceptable welfare scores (mean 67.48 ± 4.75), with major deficiencies in farm management practices, particularly regarding hygiene and space management. At T1, all farms showed substantial improvements, with an average increase of 22% in total welfare scores (mean 82.05 ± 5.71) and a mean of nine improved parameters per farm. These findings underscore the effectiveness of structured, continuous consultancy in not only resolving critical issues but also in fostering more informed, proactive, and sustainable farm management. The direct involvement of experts appears to be a key driver in enhancing both animal welfare and operational outcomes in dairy farming. Full article
(This article belongs to the Section Animal System and Management)
19 pages, 1803 KiB  
Article
Sustainable Crop Farm Productivity: Weather Effects, Technology Adoption, and Farm Management
by Sun Ling Wang, Ryan Olver and Daniel Bonin
Sustainability 2025, 17(15), 6778; https://doi.org/10.3390/su17156778 - 25 Jul 2025
Viewed by 293
Abstract
The main purpose of this study is to understand the potential determinants of sustainable field crop farm productivity. This paper considers a multi-input, multi-output production technology to estimate the effects of aridity on farm-level productivity using a stochastic input distance function. By isolating [...] Read more.
The main purpose of this study is to understand the potential determinants of sustainable field crop farm productivity. This paper considers a multi-input, multi-output production technology to estimate the effects of aridity on farm-level productivity using a stochastic input distance function. By isolating the respective weather components of agricultural total factor productivity (TFP), we can better assess the impact on productivity of adopting various technologies and farm practices that might otherwise be masked by changing climate conditions or weather shocks. We make use of data from Phase 3 of the United States Department of Agriculture (USDA) Agricultural Resource Management Survey (ARMS) between 2006 and 2020. We supplement this estimation using field crop farm productivity determinants, including technology adoption and farm practice variables derived from the ARMS Phase 2 data. We identify several factors that affect farm productivity, including many practices that help farmers make more sustainable use of natural resources. The results show that adopting yield monitoring technology, fallowing in previous years, adding or improving tile drainage, and contour farming each improved farm productivity. In particular, during our study period, conservation tillage increased by over 300% across states on average. It is estimated to increase productivity level by approximately 3% for those adopting this practice. Critically, accounting for local weather effects increased the estimated productivity of nearly all farm practices and increased the statistical significance of several variables, indicating that other TFP studies that did not account for climate or weather effects may have underestimated the technical efficiency of farms that adopted these conservation practices. However, the results also show the impacts can be heterogeneous, with effects varying between farms located in the U.S. northern or southern regions. Full article
(This article belongs to the Special Issue Sustainable Agricultural and Rural Development)
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13 pages, 1726 KiB  
Article
Assessment of Mammalian Scavenger and Wild White-Tailed Deer Activity at White-Tailed Deer Farms
by Alex R. Jack, Whitney C. Sansom, Tiffany M. Wolf, Lin Zhang, Michelle L. Schultze, Scott J. Wells and James D. Forester
Viruses 2025, 17(8), 1024; https://doi.org/10.3390/v17081024 - 22 Jul 2025
Viewed by 270
Abstract
White-tailed deer (Odocoileus virginianus) in the wild and on cervid farms have drawn the attention of state wildlife agencies and animal health agencies as Chronic Wasting Disease (CWD) has spread across North America. Deer farm regulations have been implemented to reduce [...] Read more.
White-tailed deer (Odocoileus virginianus) in the wild and on cervid farms have drawn the attention of state wildlife agencies and animal health agencies as Chronic Wasting Disease (CWD) has spread across North America. Deer farm regulations have been implemented to reduce direct contact between wild and farmed cervids; however, evidence suggests that indirect contact to infectious prions passed through the alimentary tracts of scavengers may be an important transmission pathway. The objective of this study was to characterize mammalian scavenger and wild deer activities associated with deer farms and link these activities with site-specific spatial covariates utilizing a network of camera traps, mounted to farm perimeter fences. We monitored each of 14 farms in Minnesota, Wisconsin, and Pennsylvania for two weeks during the summer, with a subset of farms also monitored in the winter and fall. Across all sites and seasons, we captured 749 observations of wildlife. In total, nine species were captured, with wild white-tailed deer accounting for over three quarters of observations. Despite the large number of wild deer observed, we found that interactions between wild and farmed deer at the fence line were infrequent (six direct contacts observed). In contrast, mammalian scavengers were frequently observed inside and outside of the fence. Supplementary cameras placed on deer feeders revealed higher observation rates of scavengers than those placed along fence lines, highlighting the potential for transmission of CWD through indirect contact via scavenger excreta. To evaluate associations between the number of observations of focal species with land-cover characteristics, two mixed-effects regression models were fitted, one model for scavengers and one for wild deer. Contrary to our hypothesis, landscape context did not have a strong impact on wildlife visitation. This suggests that farm location is less important than management practices, highlighting the need for future research into how farming practices impact rates of wildlife visitation onto cervid farms. Full article
(This article belongs to the Special Issue Chronic Wasting Disease: From Pathogenesis to Prevention)
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20 pages, 7197 KiB  
Article
Simulation of Water–Energy–Food–Carbon Nexus in the Agricultural Production Process in Liaocheng Based on the System Dynamics (SD)
by Wenshuang Yuan, Hao Wang, Yuyu Liu, Song Han, Xin Cong and Zhenghe Xu
Sustainability 2025, 17(14), 6607; https://doi.org/10.3390/su17146607 - 19 Jul 2025
Viewed by 366
Abstract
To achieve regional sustainable development, the low-carbon transformation of agriculture is essential, as it serves both as a significant carbon source and as a potential carbon sink. This study calculated the agricultural carbon emissions in Liaocheng from 2010 to 2022 by analyzing processes [...] Read more.
To achieve regional sustainable development, the low-carbon transformation of agriculture is essential, as it serves both as a significant carbon source and as a potential carbon sink. This study calculated the agricultural carbon emissions in Liaocheng from 2010 to 2022 by analyzing processes including crop cultivation, animal husbandry, and agricultural input. Additionally, a simulation model of the water–energy–food–carbon nexus (WEFC-Nexus) for Liaocheng’s agricultural production process was developed. Using Vensim PLE 10.0.0 software, this study constructed a WEFC-Nexus model encompassing four major subsystems: economic development, agricultural production, agricultural inputs, and water use. The model explored four policy scenarios: business-as-usual scenario (S1), ideal agricultural development (S2), strengthening agricultural investment (S3), and reducing agricultural input costs (S4). It also forecast the trends in carbon emissions and primary sector GDP under these different scenarios from 2023 to 2030. The conclusions were as follows: (1) Total agricultural carbon emissions exhibited a three-phase trajectory, namely, “rapid growth (2010–2014)–sharp decline (2015–2020)–gradual rebound (2021–2022)”, with sectoral contributions ranked as livestock farming (50%) > agricultural inputs (27%) > crop cultivation (23%). (2) The carbon emissions per unit of primary sector GDP (CEAG) for S2, S3, and S4 decreased by 8.86%, 5.79%, and 7.72%, respectively, compared to S1. The relationship between the carbon emissions under the four scenarios is S3 > S1 > S2 > S4. The relationship between the four scenarios in the primary sector GDP is S3 > S2 > S4 > S1. S2 can both control carbon emissions and achieve growth in primary industry output. Policy recommendations emphasize reducing chemical fertilizer use, optimizing livestock management, enhancing agricultural technology efficiency, and adjusting agricultural structures to balance economic development with environmental sustainability. Full article
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13 pages, 436 KiB  
Article
Animal Welfare Problems in Sheep Farming: A Current Overview for Germany Based on Surveys of Veterinary Offices and the Evaluation of Publicly Accessible Court Cases
by Svenja Niethammer, Sarah Schmid, Hannah Hümmelchen, Axel Wehrend and Henrik Wagner
Animals 2025, 15(14), 2116; https://doi.org/10.3390/ani15142116 - 17 Jul 2025
Viewed by 256
Abstract
This study aimed to provide an overview of the most frequent animal welfare cases documented by the authorities involved in sheep farming in Germany. A questionnaire with 26 questions was developed and sent to all German veterinary offices (supervisory authorities). Furthermore, publicly available [...] Read more.
This study aimed to provide an overview of the most frequent animal welfare cases documented by the authorities involved in sheep farming in Germany. A questionnaire with 26 questions was developed and sent to all German veterinary offices (supervisory authorities). Furthermore, publicly available court decisions on animal welfare cases over the last 30 years were consulted and analyzed. Approximately 18.1% (n = 71) of all veterinary authorities participated in the survey. The most frequently reported animal welfare cases included inadequate water supply (n = 63) and a lack of weather protection (n = 60). Other common issues were lameness (n = 56) and poor shearing management (n = 52). A total of 52 animal welfare cases presented in courts were found in the online search. The most common problems were nutrition (50%), water supply (34.6%), weather protection (32.7%), and shearing management (21.2%). These results provide information on the areas of sheep farming in which animal welfare violations occur most frequently. Based on these results, conclusions can be drawn regarding the development of preventive measures. Full article
(This article belongs to the Special Issue Advances in Small Ruminant Welfare)
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20 pages, 2217 KiB  
Article
Organic Nitrogen Substitution Enhances Carbon Sequestration but Increases Greenhouse Gas Emissions in Maize Cropping Systems
by Yanan Liu, Xiaoqing Zhao, Yuchen Cheng, Rui Xie, Tiantian Meng, Liyu Chen, Yongfeng Ren, Chunlei Xue, Kun Zhao, Shuli Wei, Jing Fang, Xiangqian Zhang, Fengcheng Sun and Zhanyuan Lu
Agronomy 2025, 15(7), 1703; https://doi.org/10.3390/agronomy15071703 - 15 Jul 2025
Viewed by 332
Abstract
Excessive chemical fertilizers degrade soil and increase greenhouse gas (GHG) emissions. Organic substitution of nitrogen fertilizers is recognized as a sustainable agricultural-management practice, yet its dual role in carbon sequestration and emissions renders the net GHG balance (NGHGB) uncertain. To assess the GHG [...] Read more.
Excessive chemical fertilizers degrade soil and increase greenhouse gas (GHG) emissions. Organic substitution of nitrogen fertilizers is recognized as a sustainable agricultural-management practice, yet its dual role in carbon sequestration and emissions renders the net GHG balance (NGHGB) uncertain. To assess the GHG mitigation potential of organic substitution strategies, this study analyzed GHG fluxes, soil organic carbon (SOC) dynamics, indirect GHG emissions, and Net Primary Productivity (NPP) based on a long-term field positioning experiment initiated in 2016. Six fertilizer regimes were systematically compared: no fertilizer control (CK); only phosphorus and potassium fertilizer (PK); total chemical fertilizer (NPK); 1/3 chemical N substituted with sheep manure (OF1); dual substitution protocol with 1/6 chemical N substituted by sheep manure and 1/6 substituted by straw-derived N (OF2); complete chemical N substitution with sheep manure (OF3). The results showed that OF1 and OF2 maintained crop yields similar to those under NPK, whereas OF3 reduced yield by over 10%; relative to NPK, OF1, OF2, and OF3 significantly increased SOC sequestration rates by 50.70–149.20%, reduced CH4 uptake by 7.9–70.63%, increased CO2 emissions by 1.4–23.9%, decreased N2O fluxes by 3.6–56.2%, and mitigated indirect GHG emissions from farm inputs by 24.02–63.95%. The NGHGB was highest under OF1, 9.44–23.99% greater than under NPK. These findings demonstrate that partial organic substitution increased carbon sequestration, maintained crop yields, whereas high substitution rates increase the risk of carbon emissions. The study results indicate that substituting 1/3 of chemical nitrogen with sheep manure in maize cropping systems represents an effective fertilizer management approach to simultaneously balance productivity and ecological sustainability. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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14 pages, 738 KiB  
Article
Assessment of Pupillometry Across Different Commercial Systems of Laying Hens to Validate Its Potential as an Objective Indicator of Welfare
by Elyse Mosco, David Kilroy and Arun H. S. Kumar
Poultry 2025, 4(3), 31; https://doi.org/10.3390/poultry4030031 - 15 Jul 2025
Viewed by 217
Abstract
Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system [...] Read more.
Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system balance and may serve as a physiological indicator of stress in laying hens. This study evaluated the utility of the IP ratio under field conditions across diverse commercial layer housing systems. Materials and Methods: In total, 296 laying hens (Lohmann Brown, n = 269; White Leghorn, n = 27) were studied across four locations in Canada housed under different systems: Guelph (indoor; pen), Spring Island (outdoor and scratch; organic), Ottawa (outdoor, indoor and scratch; free-range), and Toronto (outdoor and hobby; free-range). High-resolution photographs of the eye were taken under ambient lighting. Light intensity was measured using the light meter app. The IP ratio was calculated using NIH ImageJ software (Version 1.54p). Statistical analysis included one-way ANOVA and linear regression using GraphPad Prism (Version 5). Results: Birds housed outdoors had the highest IP ratios, followed by those in scratch systems, while indoor and pen-housed birds had the lowest IP ratios (p < 0.001). Subgroup analyses of birds in Ottawa and Spring Island farms confirmed significantly higher IP ratios in outdoor environments compared to indoor and scratch systems (p < 0.001). The IP ratio correlated weakly with ambient light intensity (r2 = 0.25) and age (r2 = 0.05), indicating minimal influence of these variables. Although White Leghorn hens showed lower IP ratios than Lohmann Browns, this difference was confounded by housing type; all White Leghorns were housed in pens. Thus, housing system but not breed was the primary driver of IP variation. Conclusions: The IP ratio is a robust, non-invasive physiological marker of welfare assessment in laying hens, sensitive to housing environment but minimally influenced by light or age. Its potential for integration with digital imaging technologies supports its use in scalable welfare assessment protocols. Full article
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20 pages, 1909 KiB  
Article
Seasonal Infective Dynamics and Risk Factors Associated with Prevalence of Zoonotic Gastrointestinal Parasites from Meat Goats in Southern Thailand
by Narin Sontigun, Chalutwan Sansamur, Tunwadee Klong-Klaew, Morakot Kaewthamasorn, Punpichaya Fungwithaya and Raktham Mektrirat
Animals 2025, 15(14), 2040; https://doi.org/10.3390/ani15142040 - 11 Jul 2025
Viewed by 460
Abstract
Gastrointestinal (GI) parasites not only significantly impact goat health and productivity but can also affect human health due to the zoonotic potential of some species. This study investigates the prevalence of internal parasites within the tropical monsoon ecosystem of southern Thailand, focusing on [...] Read more.
Gastrointestinal (GI) parasites not only significantly impact goat health and productivity but can also affect human health due to the zoonotic potential of some species. This study investigates the prevalence of internal parasites within the tropical monsoon ecosystem of southern Thailand, focusing on both phenotypic and molecular characteristics of the parasites and identifying associated risk factors in caprine farming systems. A total of 276 meat goats from Nakhon Si Thammarat province were examined, indicating an overall GI parasite prevalence of 88.8% (245/276), with strongyles and Eimeria spp. identified as the dominant parasites. In addition, mixed parasitic infections were observed in 72.2% of cases, whereas single infections comprised 27.8%. Strongyle-positive fecal samples were cultured and genetically sequenced, revealing the presence of Haemonchus contortus, Trichostrongylus colubriformis, and Oesophagostomum asperum. For associated risk factors, gender and grazing with other herds significantly impacted overall GI parasitic infections, while the gender, breed, and packed cell volume (PCV) affected the strongyle infection. A correlation analysis revealed a substantial relationship between strongyle egg per gram (EPG) counts and clinical parameters, indicating that monitoring animals with low body condition scores (BCS) and high Faffa Malan Chart (FAMACHA) scores could be an effective strategy for controlling strongyle infections. These findings highlight the importance of continued research and effective farm management practices to address strongyle infections in meat goats, improving their health and agricultural productivity in tropical regions. Moreover, the detection of four zoonotic parasites (Giardia spp., H. contortus, T. colubriformis, and Fasciola spp.) indicates the necessity for the routine surveillance and monitoring of zoonotic parasites in goats to mitigate potential human health risks. Full article
(This article belongs to the Special Issue Zoonotic Diseases: Etiology, Diagnosis, Surveillance and Epidemiology)
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19 pages, 3179 KiB  
Article
Development of a Multiplex Real-Time PCR Assay for the Detection of Eight Pathogens Associated with Bovine Respiratory Disease Complex from Clinical Samples
by Fuxing Hao, Chunhao Tao, Ruilong Xiao, Ying Huang, Weifeng Yuan, Zhen Wang and Hong Jia
Microorganisms 2025, 13(7), 1629; https://doi.org/10.3390/microorganisms13071629 - 10 Jul 2025
Viewed by 309
Abstract
Bovine respiratory disease complex (BRDC) is one of the primary causes of morbidity, mortality, and economic loss in cattle worldwide. Accurate and rapid identification of causative pathogenic agents is essential for effective disease management and control. In this study, a novel multiplex fluorescence-based [...] Read more.
Bovine respiratory disease complex (BRDC) is one of the primary causes of morbidity, mortality, and economic loss in cattle worldwide. Accurate and rapid identification of causative pathogenic agents is essential for effective disease management and control. In this study, a novel multiplex fluorescence-based quantitative polymerase chain reaction (qPCR) assay was developed for the simultaneous detection of eight major pathogens associated with BRDC. The targeted pathogens included the following: bovine viral diarrhea virus (BVDV), bovine parainfluenza virus type 3 (BPIV3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BcoV), Mycoplasma bovis (M.bovis), Pasteurella multocida (PM), Mannheimia haemolytica (MH), and infectious bovine rhinotracheitis virus (IBRV). The assay was rigorously optimized to ensure high specificity with no cross-reactivity among targets. The limit of detection (LOD) was determined to be as low as 5 copies per reaction for all target pathogens. The coefficient of variation (CVs) for both intra-assay and inter-assay measurements were consistently below 2%, demonstrating excellent reproducibility. To validate the clinical utility of the assay, a total of 1012 field samples were tested, including 504 nasal swabs from Farm A and 508 from Farm B in Jiangsu Province. BVDV, BcoV, PM, and MH were detected from Farm A, with a BVDV-positive rate of 21.63% (109/504), BcoV-positive rate of 26.79% (135/504), PM-positive rate of 28.77% (145/504), and MH-positive rate of 15.08% (76/504). Also, BcoV, PM, MH, and IBRV were detected from Farm B, with a BcoV-positive rate of 2.36% (12/508), PM-positive rate of 1.38% (7/508), MH-positive rate of 14.76% (75/508), and IBRV-positive rate of 5.51% (28/508). Notably, a significant proportion of samples showed evidence of mixed infections, underscoring the complexity of BRDC etiology and the importance of a multiplex diagnostic approach. In conclusion, the developed multiplex qPCR assay provides a reliable, rapid, and cost-effective tool for simultaneous detection of multiple BRDC-associated pathogens, which will hold great promise for enhancing disease surveillance, early diagnosis, and targeted intervention strategies, ultimately contributing to improved BRDC management and cattle health outcomes. Full article
(This article belongs to the Special Issue Animal Viral Infectious Diseases)
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13 pages, 1507 KiB  
Article
Sustainability and Innovation in Hospitality Management: Green Practices in Northeastern Hungary
by Tamás Misik and Zoltán Nagy
Sustainability 2025, 17(13), 6185; https://doi.org/10.3390/su17136185 - 5 Jul 2025
Viewed by 427
Abstract
Sustainability has also become an increasingly important issue as an international trend for the hospitality industry in recent times, with a positive message for both restaurant operators and consumers. Restaurants can become more sustainable in three main areas: (1) water and energy efficiency, [...] Read more.
Sustainability has also become an increasingly important issue as an international trend for the hospitality industry in recent times, with a positive message for both restaurant operators and consumers. Restaurants can become more sustainable in three main areas: (1) water and energy efficiency, (2) waste management, and (3) employees—social topics. This study examines the role of green practices and innovation in hospitality using three methods in parallel. In connection with a current tourism project, this paper describes some of the green practices for hospitality management in Hungary. Based on the survey, the most common sustainable practices are sourcing from local producers and using seasonal menus. The most popular food waste reduction strategies are Munch, nose-to-tail, and other food utilization options, totaling 65.0%. A total of 72.0% of consumers prefer the green restaurants. Our data show that sustainable operation is not just an environmental issue, but also increasingly a strategic business advantage. The findings are supported by the everyday practices of two of Dining Guide’s member restaurants, Iszkor and Sulyom in the Northeastern Hungary region. Both restaurants focus on locally sourced food and drink ingredients. Some dairy products, domestic fruit, and vegetables come from sustainable farming. For restaurants, adopting sustainable solutions can provide a long-term competitive advantage. Full article
(This article belongs to the Special Issue Heritage Preservation and Tourism Development)
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12 pages, 1106 KiB  
Article
Antimicrobial Resistance and Virulence Determinants of Escherichia coli Isolates from Raw Milk of Dairy Cows with Subclinical Mastitis
by Ntelekwane George Khasapane, Olga de Smidt, Kgaugelo Edward Lekota, Jane Nkhebenyane, Oriel Thekisoe and Tsepo Ramatla
Animals 2025, 15(13), 1980; https://doi.org/10.3390/ani15131980 - 5 Jul 2025
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Abstract
Subclinical mastitis (SCM) is a stealthy but devastating challenge in the dairy industry, leading to economic losses and hindering efforts to achieve milk self-sufficiency. This study investigated the prevalence of SCM, antimicrobial resistance, and virulence profiles of Escherichia coli. A total of [...] Read more.
Subclinical mastitis (SCM) is a stealthy but devastating challenge in the dairy industry, leading to economic losses and hindering efforts to achieve milk self-sufficiency. This study investigated the prevalence of SCM, antimicrobial resistance, and virulence profiles of Escherichia coli. A total of 174 milk samples were analyzed using the California mastitis test (CMT), somatic cell counts (SCCs), bacteriological culture, MALDI-TOF MS, and polymerase chain reaction (PCR). The findings revealed that the SCM prevalence was 68/174 (39.08%) based on CMT and SCC. Among SCM-positive samples, 60/68 (88.23%) were identified as E. coli, confirmed by MALDI-TOF MS and PCR assay. The most frequently detected serogroups were 0113 (11.6%) and 0113 (3.3%). Additionally, the genes for Stx1 and Stx2 were also detected in nine (15%) and one (1.7%), respectively. Antimicrobial susceptibility tests showed widespread resistance, with E. coli isolates demonstrating resistance to penicillin in 43 (71.6%), followed by ciprofloxacin in 42 (70%) and gentamicin in 18 (30%). A larger proportion of the E. coli strains (100%) harbored the blaVIM gene, while 23 (38.3%), 20%, 20%, and 1.47% contained blaKPC, blaNMD, suli1, and msrA. Thirty (50%) isolates were considered multidrug-resistant (MDR). These findings underscore the urgent need for enhanced surveillance and antibiotic stewardship in dairy farming. The presence of MDR E. coli in SCM poses a dual threat of potential transmission to humans and treatment failures in mastitis management. This study highlights the importance of proactive control strategies to mitigate the spread of antimicrobial resistance in livestock and beyond. Full article
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