Search Results (16)

The presence of mosquitoes in a certain area is sufficient evidence of the transmission of diseases, in addition to the inconvenience and harassment they cause to the population. In order for mosquito control to be successful, there must be sufficient reports and information about the extent of the distribution and spread of the mosquito in this area. This study was conducted seasonally to calculate the distribution and abundance of mosquitoes and to initially detect hepatitis C virus in the most abundant species collected in some localities: Kafr Saqr (Hanot, El-Kodah, Abo Shokok, El-Hagarsa); Abo Kebeer (Manshat Radwan, Bane Aiat, Al-Rahmania, Horbat); Diarb Negm (Safor, El-Asaied, Karmot Sahbara, Saft Zreka); and El-Zakazik (Om El-Zein, Bany Amer, Al-Zankalon, Shanbrt El-Mimona) along Sharkia Governorate. According to the results, 12 mosquito species belonging to two genera were recorded in four stations (16 sites). The species Culex pipiens was found to be the most abundant across the majority of locations. It is reaching its peak in Al-Zankalon with a maximum count of 139 ± 7.61 individuals. C. antennatus exhibited a significant level of abundance (p < 0.001), especially in Al-Rahmania (82.5 ± 4.3). Species such as C. perexiguus and An. pharoensis were found in moderate-to-low numbers. The prevailing species is C. pipiens in all locations and seasons, especially during the summer. Other species like C. antennatus and An. pharoensis are also important, although their significance is somewhat less pronounced. Clusters of sites with similar features indicate that specific locations or species exhibit consistent patterns of activity or abundance throughout several seasons. These clusters represent groups of locations that share similar characteristics, as determined by the principal components. The findings of detecting hepatitis C virus in C. pipiens (the most abundant species) collected samples showed negative results for the presence of HCV during the summer season in Sharkia Governorate. Full article

The phenomenon of seawater intrusion is becoming increasingly problematic, particularly in low-lying coastal regions and areas that rely heavily on aquifers for their freshwater supply. It is, therefore, vital to address the causes and consequences of this phenomenon in order to ensure the security of water resources and the sustainable use of water. The objective of this paper was twofold: firstly, to delineate zones with different salinization levels over time; secondly, to investigate the factors controlling seawater intrusion of the Nile Delta aquifer. Aquifer data were collected in Sharkia governorate, Egypt, over three historical periods of years: 1996, 2007, and 2018. The dataset used to create the linear model of coregionalization consisted of hydrogeological (water level), hydrodynamic (pH, EC, Na, Mg, K, Ca, HCO₃, SO₄), and auxiliary (distances from salt and freshwater sources) variables. Cokriging was applied to produce spatial thematic maps of the studied variables for the three years of the survey. In addition, factorial cokriging was applied to understand the processes beyond the change in the aquifer water quality and map the zones with similar characteristics. Results of mapping the first factor at long range over the three years indicated that there was an increase in seawater intrusion, especially in the northeastern part of the study area. The main cause of aquifer salinization over time was the depletion of the groundwater resource due to overexploitation. Full article

Tomato bacterial spots, caused by Xanthomonas campestris pv. vesicatoria (Xcv1) and X. euvesicatoria (Xe2), as well as bacterial specks, caused by two strains of Pseudomonas syringae pv. tomato (Pst1 and Pst2), represent significant threats to tomato production in the El-Sharkia governorate, often resulting in substantial yield losses. The objective of this study was to evaluate the efficacy of various biocontrol culture filtrates, including bacteria and fungi agents, in managing the occurrence and severity of these diseases, while also monitoring physiological changes in tomato leaves, including antioxidant enzymes, phenolics, and pigment content. The culture filtrates from examined Trichoderma species (T. viride, T. harzianum, and T. album), as well as the tested bacteria (Bacillus subtilis, Pseudomonas fluorescens, and Serratia marcescens) at concentrations of 25%, 50%, and 100%, significantly inhibited the proliferation of pathogenic bacteria In vitro. For the In vivo experiments, we used specific doses of 5 mL of spore suspension per plant for the fungal bioagents at a concentration of 2.5 × 10⁷ spores/mL. The bacterial bioagents were applied as a 10 mL suspension per plant at a concentration of 1 × 10⁸ CFU/mL. Spraying the culture filtrates of the tested bioagents two days before infection In vivo significantly reduced disease incidence and severity. Trichoderma viride exhibited the highest efficacy among the fungal bioagents, followed by T. harzianum and T. album. Meanwhile, the culture filtrate of B. subtilis emerged as the most potent among the bacterial bioagents, followed by P. fluorescens. Furthermore, applying these culture filtrates resulted in elevated levels of chitinase, peroxidase, and polyphenol oxidase activity. This effect extended to increased phenol contents, as well as chlorophyll a, chlorophyll b, and carotenoids in sprayed tomato plants compared to the control treatment. Overall, these findings underscore the potential of these biocontrol strategies to effectively mitigate disease incidence and severity while enhancing plant defense mechanisms and physiological parameters, thus offering promising avenues for sustainable disease management in tomato production. Full article

Seventy-eight common bean (Phaseolus vulgaris L.) seed samples were collected from twenty-one field locations across six governorates (Ismailia, Sharkia, Port Said, South Sinai, North Sinai, and Suez) in the East Delta region of Egypt between 2021 and 2022. Thirty-five Rhizoctonia solani Kühn were isolated. Sharkia had the highest percentage of R. solani isolates (RS) from the collected samples (59%), followed by Ismailia, South Sinai, Suez, North Sinai, and Port Said (49.7%, 36.7%, 33%, 29.2%, and 27.8%, respectively). Pathogenicity tests proved that the seven highest-virulence strains of R. solani had a pre-emergence damping off ranging from 17% to 30% and a post-emergence damping off ranging from 11% to 20%. The seven most pathogenic isolates were identified using the Internal Transcribed Spacer (ITS) with code numbers as follows: R. solani RSA1, R. solani RIT2, R. solani RIF3, R. solani RSoR4, R. solani RNB5, R. solani RSzA6, and R. solani RPS11. Principle component analysis (PCA), which depends on the pathogenicity result and soil variables, revealed that soil texture and soil water holding capacity (MWHC) were the most effective factors in grouping and clustering the studied R. solani isolates from the different locations into two groups. From this study, it can be established that the biodiversity of R. solani in the East Delta area of Egypt depends on changes in the environmental conditions and soil variables. The soil type and pH variables have the greatest impact on R. solani diversity, followed by the humidity-holding ability and soil porosity. Full article

Egypt’s mineral resources are an effective means of raising the country’s income. Consequently, searching for deep subsurface ore deposits is essential. In this study, we map the subsurface structure and the occurrence of related gold-ore mineralization deposits in ophiolitic-assemblage rocks around the Wadi El-Saqia area in the Central Eastern Desert of Egypt. Our approach combines airborne geophysical data (aeromagnetic mapping) and geochemical analyses. We enhanced the aeromagnetic data and interpreted them using edge-detection methods, such as the first-order vertical derivative (FVD), the analytic signal (AS), the total horizontal derivative (THD), the tilt derivative (TD), the tilt angle (TA), the theta map, both grid and porphyry analyses from the Centre for Exploration Targeting (CET), and Euler deconvolution (ED) techniques. Utilizing these methods, we located the main structural lineaments/contacts that control the distribution of hydrothermal alteration zones. In addition, our geochemical analyses use the mineral chemistry of pyroxene and plagioclase to describe their tectonic and magmatic evolution. The airborne geophysical results revealed that NW–SE, NE–SW, N–S, and E–W structural orientations are prevalent in the studied area, with depths ranging from less than 50 m to about 600 m. To validate the findings, we carried out geochemical sampling, which indicates that pyrite, galena, pyrrhotite, and electrum contain good percentages of gold (ranging from 0.01–0.09 wt%, 0.03–0.1 wt%, 0.12–0.14 wt%, and 53.55–55.01 wt%, respectively). In this study, we were thus able to find preferred locations for gold mineralization, which highlights the value of combining aeromagnetic and geochemical data for mineral exploration. Full article

The use of pesticides leads to an increase in agricultural production but also causes harmful effects on human health when excessively used. For safe consumption, pesticide residues should be below the maximum residual limits (MRLs). In this study, the residual levels of pesticides in vegetables and fruits collected from farmers’ markets in Sharkia Governorate, Egypt were investigated using LC-MS/MS and GC-MS/MS. A total number of 40 pesticides were detected in the tested vegetable and fruit samples. Insecticides were the highest group in detection frequency with 85% and 69% appearance in vegetables and fruits, respectively. Cucumber and apple samples were found to have the highest number of pesticide residues. The mean residue levels ranged from 7 to 951 µg kg⁻¹ (in vegetable samples) and from 8 to 775 µg kg⁻¹ (in fruit samples). It was found that 35 (40.7%) out of 86 pesticide residues detected in vegetables and 35 (38.9%) out of 90 pesticide residues detected in fruits exceeded MRLs. Results for lambda-cyhalothrin, fipronil, dimothoate, and omethoate in spinach, zucchini, kaki, and strawberry, respectively, can cause acute or chronic risks when consumed at 0.1 and 0.2 kg day⁻¹. Therefore, it is necessary for food safety and security to continuously monitor pesticide residues in fruits and vegetables in markets. Full article

Endophytic bacteria are plant-beneficial bacteria with a broad host range. They provide numerous benefits to their hosts, helping them tolerate several biotic and abiotic stresses. An interest has recently been developed in endophytic bacteria which are producing bioactive compounds that contribute to the biological control of various phytopathogens. This research paper aimed to investigate the potentiality of new local strains of endophytic bacteria such as Bacillus amyloliquefaciens and B. velezensis and the production of several antimicrobial metabolites associated with the biocontrol of Alternaria sp., which cause serious diseases and affect important vegetable crops in Egypt. Twenty-five endophytic bacteria isolates were obtained from different plants cultivated in El-Sharkia Governorate, Egypt. Dual culture technique was used to evaluate the bacterial isolates’ antagonistic potentiality against Alternaria sp. and Helminthosporium sp. The most active bacterial isolates obtained were selected for further screening. The antifungal activity of the most active endophytic bacterial isolate was assessed in vivo on pepper seedlings as a biocontrol agent against Alternaria sp. A significant antifungal activity was recorded with isolates C₁ and T₅ against Alternaria sp. and Helminthosporium sp. The bacterial endophyte discs of C₁ and T₅ showed the highest inhibitory effect against Alternaria sp. at 4.7 and 3.1 cm, respectively, and Helminthosporium sp. at 3.9 and 4.0 cm, respectively. The most active endophytic isolates C₁ and T₅ were identified and the 16S rRNA sequence was submitted to the NCBI GenBank database with accession numbers: MZ945930 and MZ945929 for Bacillus amyloliquefaciens and Bacillus velezensis, respectively. The deformity of pathogenic fungal mycelia of Alternaria sp. and Helminthosporium sp. was studied under the biotic stress of bacteria. The culture filtrates of B. amyloliquefaciens and B. velezensis were extracted with different solvents, and the results indicated that hexane was the most efficient. Gas Chromatography-Mass Spectrometry revealed that Bis (2-ethylhexyl) phthalate, Bis (2-ethylhexyl) ester, and N,N-Dimethyldodecylamine were major constituents of the endophytic crude extracts obtained from B. amyloliquefaciens and B. velezensis. The in vivo results showed that Alternaria sp. infection caused the highest disease incidence, leading to a high reduction in plant height and in the fresh and dry weights of pepper plants. With B. amyloliquefaciens application, DI significantly diminished compared to Alternaria sp. infected pepper plants, resulting in an increase in their morphological parameters. Our findings allow for a reduction of chemical pesticide use and the control of some important plant diseases. Full article

Campylobacter species (spp.) are one of the most important causes of human bacterial gastroenteritis in foods of animal origin. Recently, with the spread of multi-drug-resistant (MDR) and extensively drug-resistant (XDR) Campylobacter spp., natural alternative therapeutic methods are urgently required. Phytogenic active principles have gained considerable attention due to their proficiency to enhance gut health and, thereby, performance of broiler chickens. Thus, the current study aims to determine the prevalence and antimicrobial resistance of Campylobacter spp. of different chicken sources in Sharkia Governorate, Egypt, and to assess the growth-promoting, immunostimulant and antimicrobial effects of a mixture of eugenol and trans-cinnamaldehyde in an in vivo approach. A total of 101 (67.3%) campylobacter isolates was identified, according to both phenotypic and genotypic techniques. Moreover, all of the campylobacter isolates were resistant to erythromycin, trimethoprim/sulfamethoxazole, and ampicillin (100% each). Of note, a dietary supplementation of the mixture of eugenol and trans-cinnamaldehyde led to a significant improvement of the feed conversion ratio and body weight gain and a decrease in the cecal C. jejuni loads in the broilers challenged with XDR C. jejuni. Additionally, eugenol and the trans-cinnamaldehyde mixture had protective activities via the down-regulation of XDR C. jejuni (flaA, virB11 and wlaN) virulence genes and proinflammatory cytokines (TNF-α, IL-2, IL-6, and IL-8), and the up-regulation of anti-inflammatory cytokine IL-10. Thus, we recommend the usage of a mixture of eugenol and trans-cinnamaldehyde as an alternative to antimicrobials for the control and treatment of campylobacter infections. Full article

(1) Background: Childhood fever is a frequent reason for health care visits. Parents are worried about fever and its complications and show variation between their knowledge about managing fever and real practice, which are affected by many factors and beliefs. This study aimed to assess knowledge of Egyptian mothers about fever of preschool children and its domestic management and the relation between them and to identify sociodemographic factors affecting mothers’ knowledge and practice. (2) Methods: a cross-sectional study was conducted at the pediatric outpatient clinic at Zagazig University Hospitals among 297 mothers with preschool children. A structured questionnaire consisting of three parts assessed the sociodemographic characteristics, mothers’ knowledge about childhood fever, and its management practices. (3) Results: 37.7% of mothers had good knowledge about childhood fever, and 23.9% showed good management practices. Young mothers, less number of children, high education, sufficient income, and good knowledge were the significant predictors of domestic management practices towards childhood fever. (4) Conclusions: The Egyptian mothers showed insufficient levels of knowledge and domestic management practices towards preschool childhood fever. Health education interventions should be targeted to mothers to improve their knowledge and practice. Full article

Salmonella enterica is one of the most common causes of foodborne illness worldwide. Contaminated poultry products, especially meat and eggs are the main sources of human salmonellosis. Thus, the aim of the present study was to determine prevalence, antimicrobial resistance profiles, virulence, and resistance genes of Salmonella Enteritidis (S. enteritidis) and Salmonella Typhimurium (S. Typhimurium) isolated from laying hens, table eggs, and humans, in Sharkia Governorate, Egypt. The antimicrobial activity of Biosynthesized Silver Nanoparticles (AgNPs) was also evaluated. Salmonella spp. were found in 19.3% of tested samples with laying hens having the highest isolation rate (33.1%). S. Enteritidis) (5.8%), and S. Typhimurium (2.8%) were the dominant serotypes. All isolates were ampicillin resistant (100%); however, none of the isolates were meropenem resistant. Multidrug-resistant (MDR) was detected in 83.8% of the isolates with a multiple antibiotic resistance index of 0.21 to 0.57. Most isolates (81.1%) had at least three virulence genes (sopB, stn, and hilA) and none of the isolates harbored the pefA gene; four resistance genes (blaTEM, tetA, nfsA, and nfsB) were detected in 56.8% of the examined isolates. The AgNPs biosynthesized by Aspergillus niveus exhibit an absorption peak at 420 nm with an average size of 27 nm. AgNPs had a minimum inhibitory concentration of 5 µg/mL against S. enteritidis and S. typhimurium isolates and a minimum bactericidal concentration of 6 and 8 µg/mL against S. enteritidis and S. typhimurium isolates, respectively. The bacterial growth and gene expression of S. enteritidis and S. typhimurium isolates treated with AgNPs were gradually decreased as storage time was increased. In conclusion, this study indicates that S. enteritidis and S. typhimurium isolated from laying hens, table eggs, and humans exhibits resistance to multiple antimicrobial classes. The biosynthesized AgNPs showed potential antimicrobial activity against MDR S. enteritidis and S. typhimurium isolates. However, studies to assess the antimicrobial effectiveness of the biosynthesized AgNPs in laying hen farms are warranted. Full article

This paper presents a comprehensive study to assess the impact of climate change on Egypt’s water resources, focusing on irrigation water for agricultural crops, considering that the agriculture sector is the largest consumer of water in Egypt. The study aims to estimate future climate conditions using general circulation models (GCMs), to assess the impact of climate change and temperature increase on water demands for irrigation using the CROPWAT 8 model, and to determine the suitable irrigation type to adapt with future climate change. A case study was selected in the Middle part of Egypt. The study area includes Giza, Bani-Sweif, Al-Fayoum, and Minya governorates. The irrigation water requirements for major crops under current weather conditions and future climatic changes were estimated. Under the conditions of the four selected models CCSM-30, GFDLCM20, GFDLCM21, and GISS-EH, as well as the chosen scenario of A1BAIM, climate model (MAGICC/ScenGen) was applied in 2050 and 2100 to estimate the potential rise in the annual mean temperature in Middle Egypt. The results of the MAGICC/SceGen model indicated that the potential rise in temperature in the study area will be 2.12 °C in 2050, and 3.96 °C in 2100. The percentage of increase in irrigation water demands for winter crops under study ranged from 6.1 to 7.3% in 2050, and from 11.7 to 13.2% in 2100. At the same time, the increase in irrigation water demands for summer crops ranged from 4.9 to 5.8% in 2050, and from 9.3 to 10.9% in 2100. For Nili crops, the increase ranged from 5.0 to 5.1% in 2050, and from 9.6 to 9.9% in 2100. The increase in water demands due to climate change will affect the water security in Egypt, as the available water resources are limited, and population growth is another challenge which requires a proper management of water resources. Full article

Campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis in humans worldwide. Contaminated chickens and their products are the main sources of human campylobacteriosis. Therefore, this study aimed to detect the genotypic and virulence genes‘ profiles of multi-drug resistant (MDR) C. jejuni isolates and to assess the effects of sub-inhibitory concentrations (SICs) of eugenol and beta-resorcylic acid on the virulence of avian MDR C. jejuni isolates. These isolates were clustered together with the human isolates via enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting. A total of 345 samples were collected from human stool (100) and different chicken (245) samples in Sharkia Governorate, Egypt. Conventional phenotypic methods identified 113 isolates (32.8%) as C. jejuni, and all C. jejuni isolates were MDR and resistant to erythromycin and ampicillin. The genes virB11, wlaN, and flaA were detected in 52%, 36% and 100% strains, respectively. ERIC-PCR yielded 14 profiles and five main clusters. Interestingly, human and chicken C. jejuni isolates were clustered together in ERIC-PCR clusters II-V, which confirmed the genetic relatedness between the isolates from both origins. Beta-resorcylic acid and eugenol inhibited the invasion of C. jejuni isolates to chicken intestinal cells by 41.66–38.19% and 31.94–29.16%, respectively, and minimized the transcription of flaA, virB11, and wlaN genes in the tested isolates by real-time quantitative reverse transcription PCR (qRT-PCR). In essence, eugenol and beta-resorcylic acid are promising natural antimicrobials for minimizing the virulence of MDR C. jejuni in chickens, thereby managing human campylobacteriosis. Full article

Water scarcity and suitable irrigation water management in arid regions represent tangible challenges for sustainable agriculture. The current study aimed to apply multivariate analysis and to develop a simplified water quality assessment using principal component analysis (PCA) and the agglomerative hierarchical clustering (AHC) technique to assess the water quality of the Bahr Mouise canal in El-Sharkia Governorate, Egypt. The proposed methods depended on the monitored water chemical composition (e.g., pH, water electrical conductivity (ECiw), Ca²⁺, Mg²⁺, Na⁺, K⁺, HCO₃⁻, Cl⁻, and SO₄²⁻) during 2019. Based on the supervised classification of satellite images (Landsat 8 Operational Land Imager (OLI)), the distinguished land use/land cover types around the Bahr Mouise canal were agriculture, urban, and water bodies, while the dominating land use was agriculture. The water quality of the Bahr Mouise canal was classified into two classes based on the application of the irrigation water quality index (IWQI), while the water quality was classified into three classes using the PCA and AHC methods. Temporal variations in water quality were investigated, where the water qualities in winter, autumn, and spring (January, February, March, April, November, and December) were classified as class I (no restrictions) based on IWQI application, and the water salinity, sodicity, and/or alkalinity did not represent limiting factors for irrigation water quality. On the other hand, in the summer season (May, June, July, August, and October), the irrigation water was classified as class II (low restrictions); therefore, irrigation processes during summer may lead to an increase in the alkalinity hazard. The PCA classifications were compared with the IWQI results; the PCA classifications had similar assessment results during the year, except in September, while the water quality was assigned to class II using the PCA method and class I by applying the IWQI. Furthermore, the normalized difference vegetation index (NDVI) around the Bahr Mouise canal over eight months and climatic data assisted in explaining the fluctuations in water quality during 2019 as a result of changing the crop season and agriculture management. Assessments of water quality help to conserve soil, reduce degradation risk, and support decision makers in order to obtain sustainable agriculture, especially under water irrigation scarcity and the limited agricultural land in such an arid region. Full article

Motile Aeromonas septicemia is a common bacterial disease that affects Oreochromis niloticus and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of Aeromonas hydrophila complex, a total of 250 tilapia (Oreochromis niloticus) were collected randomly from 10 private tilapia farms (25 fish/farm) at El-Sharkia Governorate, Egypt. The collected fish were subjected to clinical and bacteriological examinations. The majority of infected fish displayed ulcerative necrosis, exophthalmia, and internal signs of hemorrhagic septicemia. The prevalence of A. hydrophia complex was 13.2%, where the liver was the most predominant affected organ (54.1%). Polymerase chain reaction (PCR) was used to verify the identification of A. hydrophila complex using one set of primers targeting gyrB as well as the detection of virulent genes (aerA, alt, and ahp). All isolates were positive for the gyrB-conserved gene and harbored aerA and alt virulence genes. However, none of those isolates were positive for the ahp gene. The antimicrobial sensitivity was carried out, where the recovered strains were completely sensitive to ciprofloxacin and highly resistant to amoxicillin. All retrieved strains showed the same phenotypic characteristics and were identical based on the restriction fragment length polymorphism (RFLP). Experimentally challenged fish presented a high mortality rate (76.67%) and showed typical signs as in naturally infected ones. In conclusion, the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of A. hydrophila complex. RFLP is a fundamental tool for monitoring the biodiversity among all retrieved strains of A. hydrophia. Full article