Search Results (103)

Introduction: Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a critical public health threat due to its rapid nosocomial dissemination, limited therapeutic options, and elevated mortality rates. This study aimed to characterize the epidemiology, carbapenemase profiles, and antimicrobial susceptibility patterns of CRKP isolates, as well as the clinical features and outcomes observed in infected or colonized patients. Materials and Methods: We conducted a retrospective analysis of clinical and microbiological data from patients with CRKP infections or colonization admitted between January 2023 and January 2024. Descriptive statistics were used to assess prevalence, resistance patterns, and patient outcomes. Two binary logistic regression models were applied to identify independent predictors of sepsis and in-hospital mortality. Results: Among 89 CRKP isolates, 45 underwent carbapenemase typing. More than half were metallo-β-lactamase (MBL) producers, with 44.4% co-harbouring NDM and OXA-48-like enzymes. Surgical intervention was associated with a significantly lower risk of sepsis (p < 0.01) and in-hospital mortality (p = 0.045), whereas intensive care unit (ICU) stay was a strong predictor of both outcomes. ICU admission conferred a 10-fold higher risk of sepsis (95%Cl 2.4–41.0) and a 40.8-fold higher risk of in-hospital death (95% Cl 3.5–473.3). Limitations: This single-center retrospective study included a limited number of isolates in certain groups. Additionally, cefiderocol (FDC) susceptibility was assessed by disk diffusion rather than by the broth microdilution method. Conclusions: Our study underscores the increasing prevalence of metallo-beta-lactamase-producing CRKP, particularly strains harbouring dual carbapenemases. Timely recognition of high-risk patients, combined with the implementation of targeted infection control measures and the integration of novel therapeutic options, is crucial to optimize clinical management and reduce mortality associated with CRKP. Full article

The identification of melatonin in grapes has led to the publication of numerous studies on melatonin in wines, and the aim of this study was to perform a systematic review and meta-analysis of published data on melatonin concentrations in wines. In this context, international databases such as Scopus, Web of Science and PubMed were searched for relevant articles (437) up to 29 March 2025. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used. A total of 15 studies from eight countries, involving various wine types and analytical methods, were included in the meta-analysis. Considerable analytical variation was observed across studies, and high-performance liquid chromatography (HPLC) coupled with either mass spectrometry (MS) or fluorescence (FL) detection was shown to be the most accurate and sensitive method for quantifying melatonin. The highest concentrations were found in Spanish red Tempranillo wine, Romanian white Noah wine, and Romanian rosé Lidia wine. Red wines, particularly those produced from Cabernet Sauvignon (CS) grapes, were the most frequently studied. The results of this work provide a clearer picture of melatonin levels in wine. Further research is needed to explore the implications of melatonin content in wine for human health and the wine industry. Full article

Background and Objectives: Negative-pressure wound therapy (NPWT) expedites tissue repair, yet functional recovery depends on adjunct rehabilitation. Evidence from high-resource settings is difficult to translate to Romanian county hospitals, where advanced devices are scarce. The objective of this study is to determine whether two tiers of low-technology, therapist-delivered exercise improve mobility, oedema resolution, pain and quality-of-life (QoL) beyond NPWT alone in adults with acute lower-limb wounds. Methods: A single-centre, prospective observational study (January 2021–June 2024) enrolled 92 patients and randomised them unevenly into: Group A, NPWT only (n = 39); Group B, NPWT + routine physiotherapy (n = 33); Group C, NPWT + enhanced manual programme (n = 20). All received −125 mmHg continuous suction; rehabilitation started 48 h post-operation. Primary outcomes were ankle dorsiflexion and knee flexion at 12 weeks. Secondary outcomes included calf circumference, ultrasound oedema depth, Manual Muscle Testing (MMT), pain (VAS), analgesic use and SF-36 domains through 24 weeks. Results: Baseline characteristics were similar (p > 0.40). At 12 weeks dorsiflexion reached 20.1 ± 1.8° in Group C, surpassing Group B (18.4 ± 2.1°; p = 0.004) and Group A (16.0 ± 2.3°; p < 0.001). Knee flexion followed the same gradient (140.8 ± 3.2°, 137.6 ± 3.4°, 133.4 ± 3.8° respectively). Oedema depth fell fastest in Group C (0.4 ± 0.2 mm by day 42) versus B (0.6 ± 0.2 mm) and A (0.8 ± 0.3 mm). Week-12 MMT grade ≥ 4.5 was attained by 95% of Group C, 85% of B and 72% of A (χ² = 10.9, p = 0.004). VAS pain fell more steeply with each rehabilitation layer, paralleled by a stepwise decline in daily tramadol. All SF-36 domains were highest in Group C at 24 weeks (Physical Function 88.7 ± 4.8 vs. 85.1 ± 5.4 vs. 78.2 ± 5.9; p < 0.001). Mobility correlated positively with QoL (r = 0.66) and inversely with pain and oedema. Conclusions: In a resource-constrained Romanian setting, adding structured manual physiotherapy to NPWT produced meaningful functional and patient-centred gains, while an “enhanced” programme incorporating daily PNF and elastic-band strengthening delivered the largest observed benefit. These findings justify prioritising therapist-led interventions even where sophisticated equipment is unavailable. Full article

Background: This study aimed to assess the feasibility of conducting a prospective study to evaluate histological changes in skin and muscle tissues after two weeks of balneotherapeutic intervention, as described in Romanian medical regulations. Methods: Thirty-five patients participated in this study, thirty of whom received balneotherapy (a cold mud ointment or a mud pack, or a mud bath, and/or a salted water bath) and five received hydrotherapy (the witness plot). Inclusion and exclusion criteria were applied. Twenty-four hours after discharge, a tegument and muscle biopsy were performed, and fragments were histologically prepared. Results: In the blade evidence analysis, the increased caliber and number of open capillaries and the presence of angiogenesis vessels were statistically significant (p = 0.001 < α = 0.05) for the cold mud ointment and mud pack compared with the witness and mud bath plots. The number of angiogenesis blood vessels was higher in the dermis (p < 0.05 *) with the mud pack plot and in the muscle with the mud cold ointment (p < 0.05). The histological study showed that two weeks of therapeutic intervention produced evidence-based proof in the dermis and muscle tissues, which was persistent at least twenty-four hours after the completion of the balneal course. The results encourage the team to continue the histological approach using modern techniques. Full article

Background/Objectives: Urinary tract infections (UTIs) represent a significant public health challenge, with Escherichia coli being the primary causative pathogen. The rise in antimicrobial resistance (AMR), further intensified by shifts in antibiotic prescribing practices during and after the COVID-19 pandemic, poses substantial difficulties in treatment optimization and clinical management. Methods: This retrospective study analyzed 644 E. coli strains from urine samples collected in a southeastern Romanian hospital during two periods: pre-pandemic (2018–2019, N = 361) and post-pandemic (2023–2024, N = 283). Antimicrobial susceptibility was assessed using the VITEK automated system for key antibiotic classes. Results: A significant increase in fluoroquinolone resistance was observed, especially for ciprofloxacin (p = 0.02), alongside rising ceftriaxone resistance (p = 0.004), suggesting the spread of ESBL-producing strains. Resistance to trimethoprim/sulfamethoxazole, ampicillin, and amoxicillin/clavulanic acid remained high, limiting their empirical use. Carbapenem resistance was low (p > 0.1), while nitrofurantoin and fosfomycin retained high efficacy (p = 0.26 and p = 0.64). Conclusions: The post-pandemic period showed a concerning rise in resistance to fluoroquinolones and third-generation cephalosporins, highlighting the need for stricter antimicrobial stewardship. Carbapenems remain effective for severe infections, while nitrofurantoin and fosfomycin are reliable first-line options for uncomplicated UTIs. Continuous AMR surveillance is essential to optimize treatment and curb multidrug-resistant strains. Full article

The annual rise in antimicrobial resistance (AMR) is a major public health concern. This Romanian questionnaire-based survey aimed to evaluate the appropriateness of routine antimicrobial use by veterinary practitioners in food-producing animals and to gauge their concerns about AMR. To gather data, an online questionnaire with 28 questions was developed and distributed nationwide to veterinarians via the Google Drive network and Google Forms. Despite this increased awareness, deviations in antimicrobial administration persist, often due to insufficient knowledge about AMR and the mechanisms of antimicrobial action. This study underscores the urgent need for prudent antimicrobial use in livestock production in Romania. Full article

This research paper explores Romanian Orthodox religious places as vital centers for producing and promoting national identity as well as cultural and religious heritage in Italy. Through the application of a spatial perspective, it addresses the complexities of heritage recognition, questioning what constitutes “heritage” for the religious minorities in Italy and highlighting the inadequacies of the current legal frameworks in this context. The paper focuses on the interplay between history and memory, scrutinizing the dialectical relationships that shape polyphonic, collective, and public memories of the Romanian parishes’ national and religious heritage. Moreover, it analyzes how memories, traditions, and national identity influence the perception of religious communities by focusing on constructing a group memory that highlights ethnic identity rather than religious affiliation. Full article

Over the past 30 years, Romanian Educational Research (RER) has strived to enhance its global visibility. This study examines the evolution and structure of RER through a bibliometric analysis of 10,396 publications indexed in the Web of Science Core Collection from 1975 to 2024. The results reveal fluctuating productivity, with growth driven by national and international initiatives, and challenges during the COVID-19 pandemic. Conference papers dominate research output, while articles and reviews are less frequent. Although domestic sources offer valuable publication opportunities, increasing visibility in high-impact journals is essential for international recognition. Universities across various regions contribute significantly, with research intensive institutions producing the bulk of articles and reviews. Five primary research clusters emerged, including studies on student performance factors, digital transformation in education, curriculum development and students’ skills, competency-based education and teacher training, as well as advancements in teaching, learning, and assessment practices. The study highlights the importance of international collaborations, particularly with institutions in Europe, the United States, Canada, and Australia, to boost the worldwide impact of Romanian research. These findings provide insights into the current state of RER and suggest that fostering international partnerships and leveraging local strengths can enhance its global presence and contribute to academic diversity. Full article

This paper aims to highlight the specific elements regarding emergence and resilience in implementing integrated management systems (IMSs) in organizations with an industrial profile in Romania. The purpose of the research was to carry out an extensive study in the industrial field to identify key elements in the implementation of IMSs, considering the identification and analysis of those elements that can generate risks (emerging elements) as well as the identification and analysis of components that can be used to intervene quickly in the event of the manifestation of risks and that may produce unexpected disturbances (resilient management). A quantitative survey distributed to 147 managers of an industrial organization that has a minimum of two integrated management systems was used for data collection. The present study highlights the opinions of the managers from Romanian industrial enterprises regarding the elements that contribute when implementing an integrated management system. For this purpose, the survey focused on the following main topics: the advantages achieved from the integration, the internal and external barriers encountered, the strategies followed for integration, the risks that occurred, some of the internal and external performance indicators (KPIs) that guide the organization, the elements that are not susceptible to integration, the integration model used, the audit typology, the documents that are needed and the main aspects improved after implementing the integrated management system. The information obtained from the study outlined several themes that management should consider in order to obtain a framework for integration guidance. Full article

Forest attributes, such as standing stock, diameter at breast height (DBH), tree height, and basal area, are critical for effective forest management; yet, traditional estimation methods remain labor-intensive and often lack the spatial detail required for contemporary decision-making. This study addresses these challenges by integrating machine learning algorithms with high-resolution remotely sensed data and rigorously collected ground truth measurements to produce accurate, national-scale maps of forest attributes in Romania. To ensure the reliability of the model predictions, extensive field campaigns were conducted across representative Romanian forests. During these campaigns, detailed measurements were recorded for every tree within selected plots. For each tree, DBH was measured directly, and tree heights were obtained either by direct measurement—using hypsometers or clinometers—or, when direct measurements were not feasible, by applying well-established DBH—height allometric relationships that have been calibrated for the local forest types. This comprehensive approach to ground data collection, supplemented by an independent dataset from Brasov County collected using the same protocols, allowed for robust training and validation of the machine learning models. This study evaluates the performance of three machine learning algorithms—Random Forest (RF), Classification and Regression Trees (CART), and the Gradient Boosting Tree Algorithm (GBTA)—in predicting the forest attributes from Sentinel-2 satellite imagery. While Random Forest consistently delivered high R² values and low root mean square errors (RMSE) across all attributes, GBTA showed particular strength in predicting standing stock, and CART excelled in basal area estimation but was less reliable for other attributes. A sensitivity analysis across multiple spatial resolutions revealed that the performance of all algorithms varied significantly with changes in resolution, emphasizing the importance of selecting an appropriate scale for accurate forest mapping. By focusing on both the methodological advancements in machine learning applications and the rigorous, detailed empirical forest data collection, this study provides a clear solution to the problem of obtaining reliable, spatially detailed forest attribute maps. Full article

In Romanian ethnopharmacology, Eupatorium cannabinum species is known for its remarkable biological activity. We present an advanced approach to encapsulation using maltodextrin matrices to enhance the stability and efficacy of phytoconstituents and nanoparticles. Two distinct carrier systems were developed: (i) a direct micro-spray encapsulation of E. cannabinum in maltodextrin to produce a maltodextrin-encapsulated carrier (MEC), and (ii) a two-step process involving the preparation of a new phytocarrier system based on gold nanoparticles (EC-AuNPs), followed by micro-spray encapsulation in maltodextrin to create the maltodextrin-encapsulated AuNPs system (MEC-AuNPs system). Comprehensive chemical profiling using GC–MS and ESI–QTOF–MS revealed 80 bioactive molecules, including terpenoids, alkaloids, flavonoids, and phytoecdysteroids. Morpho-structural (XRD, FTIR, Raman spectroscopy, SEM) and thermal analyses confirmed the successful integration of NPs within the matrices. EC-AuNPs and MEC-AuNPs exhibited superior antioxidant activity, significant antimicrobial efficacy against major bacterial pathogens (S. aureus, B. subtilis, B. cereus, P. aeruginosa, S. typhi, and E. coli), and enhanced cytotoxicity against MCF-7 and HT-29 cancer cell lines. This study highlights the potential of combining E. cannabinum with AuNPs and maltodextrin encapsulation to develop multifunctional therapeutic systems. The findings underscore the importance of phytoconstituent stabilization and nanotechnology in addressing global antimicrobial resistance and advancing innovative medical applications. Full article

The study of honey in Iasi County reveals its ecological, economic and health importance, emphasizing its unique properties, role in biodiversity and value in promoting sustainable beekeeping and regional identity. This study aimed to investigate the characteristics of honey from Iasi County, Romania, analyzing 27 samples collected in 2020 and 2021. The samples include tilia (8 raw, 7 commercial), acacia (2 raw, 2 commercial), rapeseed (3 raw), sunflower (3 raw) and lavender (2 raw) honey. Analyses were carried out under Romanian/EU standards, assessing parameters such as color, electrical conductivity, moisture, total soluble solids (TSS), acidity (free, lactone, total), pH, hydroxymethylfurfural (HMF), ash and mineral composition (Na, K, Ca, Mg, Cu, Zn, Mn and Fe). The results revealed significant differences between raw and commercial honeys. Notably, in commercial tilia honey, higher values were found for color (38.58 mm Pfund vs. 24.14 mm Pfund), total acidity (25.93 meq·kg⁻¹ vs. 17.36 meq·kg⁻¹) and HMF levels (8.84 mg·kg⁻¹ vs. 3.68 mg·kg⁻¹). Conversely, water-insoluble solids (0.08% vs. 0.15%) and ash content (0.21% vs. 0.30%) were lower in commercial samples. Potassium was the most abundant mineral detected, while copper and zinc levels were the lowest. Significant correlations were observed between several parameters, including ash with electrical conductivity and HMF with acidity. This study underscores the impact of processing on honey quality and highlights the importance of understanding honey composition for consumers and producers alike. Full article

Transformer models produce advanced text representations that have been used to break through the hard challenge of natural language understanding. Using the Transformer’s attention mechanism, which acts as a language learning memory, trained on tens of billions of words, a word sense disambiguation (WSD) algorithm can now construct a more faithful vectorial representation of the context of a word to be disambiguated. Working with a set of 34 lemmas of nouns, verbs, adjectives and adverbs selected from the National Reference Corpus of Romanian (CoRoLa), we show that using BERT’s attention heads at all hidden layers, we can devise contextual vectors of the target lemma that produce better clusters of lemma’s senses than the ones obtained with standard BERT embeddings. If we automatically translate the Romanian example sentences of the target lemma into English, we show that we can reliably infer the number of senses with which the target lemma appears in the CoRoLa. We also describe an unsupervised WSD algorithm that, using a Romanian BERT model and a few example sentences of the target lemma’s senses, can label the Romanian induced sense clusters with the appropriate sense labels, with an average accuracy of 64%. Full article

Forests mitigate greenhouse gas (GHG) emissions by capturing CO₂ and storing it as carbon in various forms, including living biomass, dead wood, soil, and forest litter. Importantly, when trees are harvested, a portion of the above-ground biomass is converted into harvested wood products (HWPs), which can retain carbon for decades. With approximately 7 million hectares of forest (30% of its land area), Romania significantly contributes to the country’s carbon budget through the HWP pool. Using country-specific data from 1961 to 2022 and an IPCC method, we tracked HWP carbon storage and projected future scenarios to evaluate the category’s significance in achieving the 2050 climate target. During this period, the carbon stored in Romanian HWPs more than doubled from 28.20 TgC to 60.76 TgC, with sawnwood products as major contributors. Fluctuations were influenced by domestic policies, market dynamics, and industry changes, notably after the 1990s. Annual carbon inflow dipped to 0.65 TgC in 1994 and peaked at 2.54 TgC in 2013. By analyzing the scenarios, we demonstrated that a moderate growth trajectory in carbon inflow, combined with a focus on producing long-lived wood products, could double carbon stock changes by 2050 to 4.4 TgC—roughly 4% of the country’s current total emissions excluding the LULUCF sector. Additionally, based on sustainable forest management practices in Romania, this approach would significantly enhance the carbon pool and its importance in achieving the country’s climate policies. Full article

The present study is concerned with some aspects of the production of [ɨ] and [ə] in Romanian, i.e., their position within the vowel space, degree of acoustic variability and acoustic duration. To this end, acoustic data were collected for the Romanian stressed vowels [i e a o u ɨ ə] produced by six speakers in controlled consonantal context conditions and real and nonsense words. The formant frequency data reveal that [ɨ] and [ə] do not overlap along the F1 dimension, which may be ascribed to the need to set in contrast the two central vowels phonologically. Moreover, [ɨ] is clearly more variable in F2, and thus in vowel fronting, than schwa. Regarding segmental duration, [ɨ] is as short as high vowels and shorter than schwa, whose duration is comparable to that of mid vowels. The phonetic characteristics for stressed schwa in Romanian are in contrast with those for the same vowel in other world’s languages, in which it is highly variable and shorter than all or most peripheral vowels. This behaviour may be attributed to the existence of two central vowels, while suggesting that [ə] has a well-defined articulatory target in the Romanian language. Full article