Search Results (24,760)

The emergence of variant strains of Avian orthoreovirus (ARV) has caused substantial economic losses in the poultry industry worldwide, but the molecular features of goose-origin strains and viral transmission among different avian species remain poorly understood. Here, we describe a goose-origin avian orthoreovirus strain, SD0407, associated with growth retardation and joint swelling. Complete genome analysis identified ten double-stranded RNA segments. Sequence comparison indicated that SD0407 is closely related to previously reported duck-origin reovirus strains. Phylogenetic and recombination analyses showed that most segments clustered with duck-origin strains, indicating close genetic relatedness among waterfowl-origin orthoreoviruses. Sequence and structural analysis of the σC attachment protein revealed ten unique amino acid substitutions, including D250 within the DE-loop region involved in receptor-binding. Molecular docking suggested that σC interacts with the conserved AnxA2-S100A10 heterotetrameric receptor complex, providing a possible structural basis for receptor compatibility across avian species. Although SD0407 replicated efficiently in goose embryo fibroblasts, it did not induce expression of type I, II or III interferons. Transcriptome profiling revealed weak activation of innate immune signaling and downregulation of metabolic and cytoskeletal genes, consistent with effective suppression of antiviral responses. These findings demonstrate that SD0407 combines structural variability with immune evasion to enhance host adaptability and underscore the importance of sustained ARV surveillance in waterfowl populations. Full article

Within a waste biorefinery framework, integrating agro-industrial by-products into the circular economy requires a detailed understanding of the thermochemical conversion behaviour of low-grade carbonaceous materials. This study evaluates the co-pyrolysis characteristics of Soma lignite (SL) and pectin-rich sugar beet pulp (SBP) as a sustainable route for upgrading these resources into clean energy carriers. Interactions between the two feedstocks were analysed by thermogravimetric measurements, triple-region kinetic modelling, and quantitative synergy indices at six mixing ratios, including the pure samples (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100 wt% SL:SBP). The Reactivity Index ($R_{\mathrm{m}}$) increased from $0.97\times {10}^{-4}$ s${}^{-1}$K${}^{-1}$ for pure SL to $8.65\times {10}^{-4}$ s${}^{-1}$K${}^{-1}$ for the 20:80 blend, showing that SBP acts as a highly reactive biomass component that accelerates devolatilisation in the main pyrolysis region. Synergy analysis indicated a shift from inhibitory behaviour in coal-rich blends to slightly positive synergy in SBP-rich mixtures, with the onset of positive $\Delta TC$ around 60 wt% SBP under the present single-heating-rate, non-replicated TGA conditions. This tentative threshold-like behaviour suggests that a critical level of literature-supported, hypothesised hydrogen-donating biomass radicals may be required to overcome the structural resistance of the coal matrix. Within these experimental limitations, the apparent macro-kinetic deviations and first-order Arrhenius parameters suggest that SL/SBP co-pyrolysis follows a complex, non-additive pathway that should be further validated by multi-heating-rate and product characterisation studies in future work. The primary contribution of this work lies in proposing this distinct threshold-like biomass fraction at the macro-kinetic level that governs the transition from heat-transfer-limited antagonism to radical-influenced synergy in low-rank coal and pectin-rich biomass blends. Overall, the combined $\Delta TC$, $\Delta E$ and $R_{\mathrm{m}}$ descriptors provide useful macro-kinetic benchmarks for guiding the optimisation of thermochemical processes for low-grade carbonaceous resources. Full article

Eye movements are difficult to observe and replicate, making them a promising yet understudied modality for behavioral biometrics. This study is the first to examine the feasibility of using eye movement patterns during manga reading as a biometric identifier, leveraging the medium’s rich behavioral data from diverse reading behaviors. Eye movement data from 59 participants were recorded while they read two manga works on a screen. A comprehensive set of gaze features was extracted and evaluated using five machine learning classifiers, among which Random Forest (RF) consistently achieved the best performance. Under constrained experimental conditions, the RF classifier achieved a Rank-1 identification rate of 95.0% and an equal error rate (EER) of 1.9%. Furthermore, this study systematically investigated two critical challenges for practical deployment: stimulus dependency and template aging. Cross-stimulus evaluation revealed substantial performance degradation when training and testing used different manga works, and template aging analysis over an approximately 90-day interval demonstrated notable declines in identification accuracy. These results provide preliminary evidence supporting the potential of natural reading behaviors for biometric continuous authentication systems while highlighting the need for further research into cross-stimulus generalization and temporal stability. Full article

Epstein–Barr virus (EBV) establishes lifelong infection in most humans, yet its biology in immunocompetent hosts is commonly framed as a binary alternation between latency and productive lytic replication. Accumulating molecular and single-cell evidence challenges this view, indicating that EBV frequently enters abortive forms of lytic reactivation that do not culminate in virion production. Here, we propose a conceptual framework in which EBV persistence is governed by feedback-regulated interactions and permissive conditions for reactivation rather than a strictly sequential life cycle. Immediate-early and early gene expression can be repeatedly induced by inflammatory signaling, cellular stress, and epigenetic changes. However, progression to viral DNA replication represents a highly functional barrier that likely requires the coordinated convergence of multiple viral and host conditions. Failure to reach this threshold arrests reactivation before late gene expression, generating a stable partial lytic state characterized by sustained immunomodulatory viral protein expression without the production of infectious particles. Immune surveillance reinforces this bottleneck by eliminating cells undergoing full lytic replication while sparing those stalled in early phases. We argue that EBV persistence reflects a dynamic equilibrium shaped by regulatory interactions between viral gene expression and host immunity, with implications for biomarker interpretation and therapeutic strategies in chronic inflammatory and autoimmune disease. Full article

Wine bottle aging is governed by complex redox reactions involving phenolic compounds, oxygen transfer and storage conditions, which collectively determine the evolution of wine composition and sensory properties. This review critically examines the main oxidative mechanisms responsible for bottle aging and evaluates traditional and emerging strategies aimed at modulating the evolution of wine. Particular attention is paid to oxygen management, cork type, temperature and light exposure, as well as alternative approaches such as accelerated aging techniques and underwater storage. The available evidence suggests that most accelerated aging technologies fail to replicate the chemical pathways of natural in-bottle aging, often resulting in different aromatic profiles. Attention is paid to underwater aging, an emerging practice that combines specific conditions of temperature, light and limited oxygen availability. The results of the available studies indicate that underwater aging does not significantly alter the basic chemical parameters of wine, but can modulate its phenolic, chromatic and sensory evolution, suggesting a slowdown in oxidative processes compared to traditional aging in the cellar. Full article

Water deficit is a major abiotic constraint limiting peanut (Arachis hypogaea L.) production. This study evaluated the combined effects of filter cake, foliar application of an amino acid-based biostimulant, microbial consortium inoculation, on peanut growth, physiology, and yield under water scarcity conditions. Treatments were arranged in a split-plot design with four replicates, where filter cake (0 and 5 t ha⁻¹) was assigned to main plots, amino acid application to subplots (0.25 and 0.50 L ha⁻¹), and microbial consortium to sub-subplots (100 and 200 mL m⁻²). At 50 days after sowing, plant growth parameters, relative chlorophyll content, and aboveground biomass were assessed, while yield components and seed yield were determined at harvest. Results indicated that the combined treatment with 5 t ha⁻¹ filter cake, 0.50 L ha⁻¹ amino acids, and 200 mL m⁻² microbial consortium, consistently produced the highest main stem length (increase of 40%), aboveground biomass accumulation (increase of 41%), number of matured pods per plant (increase of 38%), seed mass per plant (increase of 87%), and final seed yield (increase of 86%) compared to the lowest-input treatment (F₀A_0.25M₁₀₀) under water-limited conditions. These findings indicate that the integrated fertilization can improve phenological, physiological, and yield responses and represents a sustainable approach to improve peanut resilience and productivity under water scarcity. Full article

Virtual reality (VR) has emerged as a technology with growing presence in education, driven by its potential to increase motivation, promote learning, and offer immersive experiences that are challenging to replicate in traditional settings. However, the literature shows contradictory results regarding its impact on academic performance, cognitive load, and student attitudes, particularly when comparing immersive and non-immersive (desktop) modalities. Against this backdrop, this study aimed to examine whether interaction with VR-based learning objects improves knowledge acquisition, whether differences exist between immersive and desktop versions, what cognitive load is associated with each modality, and what attitudes students develop toward VR. A total of 136 Education students participated, randomly assigned to either the immersive (n = 70) or non-immersive (n = 66) condition, following a pretest–posttest experimental design. Data were collected using a performance test, the NASA-TLX questionnaire, and a semantic differential scale. Results indicated significant improvements in learning across both modalities with no statistically significant differences between them, a slightly higher—yet low-to-moderate—cognitive load in the immersive condition, and highly positive attitudes in both groups. These findings suggest that both modalities are effective and well accepted, although immersive VR requires somewhat greater cognitive effort. The discussion highlights the need to clarify the factors that moderate these effects and to advance theoretical frameworks for instructional design in VR environments. Full article

Viral infections remain a global public health challenge. Stimulating the innate immune system is a potent therapeutic strategy that promotes pathogen clearance, directly impacting disease severity and clinical outcomes. Interferons and interferon-stimulated genes (ISGs) are critical components of this antiviral defense system. Neomycin, an aminoglycoside antibiotic, can induce ISG expression and help establish an antiviral state. In this study, we demonstrated that neomycin induces the production of pro-inflammatory cytokines (IL1β, TNFα, IL6, GM-CSF, and IFN-γ) in peripheral mononuclear blood cells (PBMCs) and activates key antiviral ISGs, including MxA, OAS1, and IRF7. The protein expression profiles elicited by neomycin were comparable to those induced by poly(I:C). Intranasal delivery of neomycin to CBA and BALB/c mice induced various ISGs in both the respiratory tract and splenic tissues. Prophylactic administration of neomycin significantly inhibited influenza B virus replication in the lung and nasal turbinates of CBA mice in a sublethal infection model. Overall, our data suggest that neomycin, when used prophylactically alone or combined with other antiviral strategies, shows considerable potential for the attenuation of influenza B virus infections. Full article

The widespread occurrence of macrolide antibiotics (MLs) in aquatic environments poses potential ecological risks; however, the interactive effects of MLs, especially combined MLs on microalgae and their removal mechanisms, remain poorly understood. This study investigated the removal efficiency, physiological–biochemical responses, and molecular mechanisms of Chlorella pyrenoidosa under single and combined exposure to erythromycin (ERY) and roxithromycin (ROX) over 14 days. The results demonstrated that antibiotic removal efficiency was concentration-dependent and higher in low-concentration treatment. The removal rates of ERY (0.15 mg/L) and ROX (0.02 mg/L) reached 100% and 66.86%, respectively. Notably, in the combined low-concentration group, the presence of ROX promoted the degradation of ERY, with the removal being 11.06–14.77% higher than in single treatment. Conversely, in high-concentration combined treatments (1.63 mg/L ERY + 0.5 mg/L ROX), the removal of ERY was inhibited and the removal of ROX was comparable with the corresponding single treatment. High-concentration treatment groups and combined-treatment groups significantly inhibited microalgae growth and total chlorophyll content, modified the chlorophyll composition, and induced severe oxidative stress. Correlation analysis revealed that antibiotic removal was positively correlated with cell density, chlorophyll content, CAT, CYP450, and GST activities while negatively correlated with SOD, ROS, and MDA. Transcriptomic analysis revealed significant disruption of xenobiotic metabolism pathways, photosynthesis-related processes, and DNA replication/mismatch repair pathways. Key genes involved in stress signaling (e.g., MKK3, MPK3), detoxification (e.g., CYP97, GSTP), and photosynthesis (e.g., HemL) were differentially regulated, providing molecular evidence for the observed physiological responses and removal behaviors. These findings provide valuable insights for the ecological risk assessment of antibiotic mixtures and the development of microalgae-based wastewater treatment technologies. Full article

Constructing resilient Ecological Security Patterns (ESPs) in polycentric urban agglomerations is computationally challenging due to persistent scale mismatches between local planning and regional strategies. To address this, we developed a novel Proactive Integration Mechanism (PIM), a computational framework that dynamically optimizes ESPs by algorithmically fusing multi-source geospatial data. The PIM integrates three innovative components: (1) a Function–Structure–Policy data fusion approach that couples Self-Organizing Map clustering of ecosystem services with Morphological Spatial Pattern Analysis and policy data to identify ecological sources; (2) a Dual-Feedback Mechanism that hybridizes circuit theory with an Improved Ant Colony Optimization algorithm for dynamic corridor delineation; and (3) complex network analysis to derive targeted interventions from topological properties. Applied to a node city of the Chengdu-Chongqing Economic Circle, the PIM identified 22 integrated ecological sources and 37 corridors. The optimized network showed enhanced resilience: a deterministic 20.5% increase in circuit redundancy (α-index) and an 8.6% improvement in overall connectivity (γ-index), achieved through minimal topological modifications. Temporal validation (2000–2020) confirmed the high stability of the identified patterns. This study provides a potentially replicable and computationally robust framework that bridges spatial ecology with optimization algorithms, offering a promising paradigm for constructing ESPs in node cities within subtropical urban agglomerations. Full article

Members of the genus Brucella are bacterial pathogens of global importance, and their increasing detection in marine mammals has raised concerns for wildlife conservation and public health. In this study, we evaluated the biological and pathogenic characteristics of two Brucella sp. sequence type 27 (ST27) isolates obtained from a dwarf sperm whale (Kogia sima). We compared them with terrestrial and marine Brucella reference strains. We assessed resistance to polymyxin B and human serum complement, intracellular infection dynamics in HeLa epithelial cells, persistence in a murine model, and associated hematological and histopathological changes, and analyzed lipopolysaccharide (LPS) profiles. The Kogia isolates exhibited resistance to polymyxin B and serum complement, comparable to that of B. abortus 2308W and marine mammal Brucella strains. In HeLa cells, the isolates displayed distinct, strain-specific intracellular infection dynamics. In the murine model, both isolates persisted in the spleen and induced granulomatous lesions. However, splenic bacterial loads and histopathological scores were generally lower than those observed with B. abortus 2308W, which exhibited the highest virulence among the strains evaluated. Hematological alterations associated with Kogia isolates were also less pronounced than those induced by B. abortus 2308W, indicating an intermediate and strain-dependent virulence phenotype without evidence of enhanced virulence relative to the terrestrial reference strain. Western blot analyses showed that Brucella sp. ST27 isolates were not recognized by anti-B. abortus or anti-O-antigen monoclonal antibodies, while exhibiting a distinct recognition pattern with anti-B. canis serum, indicating differences in surface antigen composition. Comparative whole-genome analysis identified a limited number of isolate-specific variants affecting coding and intergenic regions. Collectively, these findings highlight phenotypic and genetic features of Brucella sp. ST27 from Kogia sima, which distinguishes it from other marine and terrestrial Brucella strains and supports further investigation into its biological behavior and potential public health relevance. Full article

This paper provides a historical perspective on comparatively low levels of trade in the Middle East and North Africa (MENA) region, focusing on studies addressing the impact of the Israeli–Palestinian conflict. Our literature review identifies best practices and reviews trade potential estimates and finds that the last year for which a relevant trade potential estimate for the region accounting for the influence of the Israeli–Palestinian conflict is available is 1999. First, we replicate the seminal study that provided the earliest estimation of trade potential. Next, we extend and update this study, using a best practice panel PPML gravity model with ex(/im)porter-year fixed effects for 76 countries (1991–2019 inclusive). Finally, we use two alternative approaches to estimate the intra-MENA trade potential that could have been reaped as a consequence of a geopolitically more stable and open Middle East (ME). In the year 2019, this ‘pot of gold’ (POG) in per cent of intra-MENA trade amounted to 10% to 54% (import-based) and 21% to 48% (export-based), substantially lower than earlier literature reports. Full article

The retinoblastoma (RB) protein was the first tumor suppressor discovered and has been extensively studied for its canonical role in cell-cycle regulation. However, RB has broader noncanonical roles in DNA damage repair, chromosomal stability, apoptosis control, lineage commitment, cell differentiation and broad transcriptional regulation. Historically, RB inactivation has been associated with tumorigenesis, as well as resistance to cyclin-dependent kinase 4/6 inhibitors (CDK4/6i), leading to its investigation as a potential predictive biomarker. However, clinical data have not demonstrated that RB function reliably predicts response to CDK4/6i consistently. These discrepancies highlight the need to reconsider RB’s role in therapeutic response, as RB loss can promote replication stress, induce chromosomal instability, and lead to transcriptional reprograming, potentially generating context-specific therapeutic vulnerabilities. In this review, we examine the multifaceted biology of RB and evaluate how its loss influences responses to chemotherapy and targeted therapies. We highlight emerging strategies that exploit RB-deficient states using rational monotherapy and combination approaches. Reframing RB dysfunction from a binary biomarker to a driver of exploitable cellular vulnerabilities may inform and expand precision oncology strategies for aggressive and treatment-resistant cancers. Full article

The capsid protein pE120R of African swine fever virus (ASFV) is highly immunogenic and is thought to play an important role in viral replication, yet its molecular characteristics and functions during infection remain poorly understood. Here, we generated two monoclonal antibodies (mAbs), 1C11 and 3G7, against ASFV pE120R and characterized their specificity and utility. Epitope mapping showed that 1C11 recognized the linear epitope ¹⁰⁹KKHLFP¹¹⁴, whereas 3G7 recognized ¹¹²LFPKL¹¹⁶. These antibodies enabled analysis of pE120R expression and localization during ASFV infection, demonstrating that pE120R is expressed at a late stage and partially co-localizes with the structural protein p54 in viral factories. Together, these results provide valuable immunological tools for further investigation of pE120R in ASFV replication and pathogenesis. Full article

Background/Objectives: Digital health ecosystems increasingly integrate content, behavioral interventions, and commercial offerings across multiple platforms. While design consistency is established as critical for trust in commercial contexts, its associations with health behavior change and objective health outcomes remain underexplored. This study examined how cross-platform design consistency and aesthetic experience are associated with behavioral adoption through psychological pathways and investigated relationships between design-driven adoption and objective health outcomes. Methods: A convergent mixed-method design comprised five integrated studies: systematic content analysis of short-form videos (N = 200), expert evaluation and user testing (N = 33), a cross-sectional survey (N = 186), semi-structured interviews (N = 15), and a 3-month longitudinal health outcome analysis (N = 143). Structural equation modeling tested pathways from design features through psychological mediators and COM-B components (capability, opportunity, motivation) to behavioral adoption and health outcomes. Results: Design consistency was significantly associated with trust (β = 0.52), perceived value (β = 0.68), and reduced perceived risk (β = −0.41; all p < 0.001). Aesthetic experience predicted emotional resonance (β = 0.71, p < 0.001) and moderated design–trust associations. COM-B components mediated 75% of the intention-to-adoption pathway (total indirect effect = 0.51, p < 0.001). High-adoption users showed clinically meaningful improvements in weight (−2.8 kg, d = 0.89), HbA1c (−0.7%, d = 0.65), fasting glucose (−0.9 mmol/L, d = 0.72), and LDL-C (−0.4 mmol/L, d = 0.51) over three months. Conclusions: Within a single, influencer-centered Chinese digital health ecosystem, design consistency and aesthetic experience were significantly associated with trust, psychological readiness, and behavioral adoption. These findings are observational; randomized controlled trials and multi-site replication are required to establish causal mechanisms and assess generalizability. Full article