Search Results (261)

Porcine circoviruses are significant pathogens that affect swine populations worldwide, with implications for animal health and productivity. While PCV2 is well-documented, particularly due to widespread vaccination programs, PCV3 is less understood, and its epidemiological impact is still under investigation. This study screened for PCV2 and PCV3 in pigs and wild boars across Portugal to assess their prevalence. Also, nucleotide sequence determination was performed to evaluate the genetic diversity of these viruses. Stool samples from 160 pigs belonging to different groups (quarantine, nursery, fattening and adult pigs), as well as organ samples from 120 hunted wild boars, were analyzed. Samples were collected from twelve of the eighteen mainland Portuguese districts with positive cases being detected in nine of them. Pigs had a lower prevalence of PCV2 (1.9%) than PCV3 (11.2%), but the opposite was true in wild boars (76.7% for PCV2 and 55.0% for PCV3). The lower PCV2 prevalence in pigs can be attributed to the PCV2 vaccination program implemented. Additionally, these viruses were significantly more prevalent in wild boars (90.8% were infected with at least one of the viruses) than in domestic pigs (only 12.5%). This significant difference highlights the impact of the controlled environment in pig farms on disease prevention in contrast to the higher exposure risks faced by wild boars in their natural habitat. Compared to a previous study from 2023, we observed a slight decrease in the percentage of positive cases for both PCV2 and PCV3. Phylogenetic analysis of sequences obtained by Sanger sequencing allowed us to conclude that the samples from domestic pigs belong to the PCV2a and PCV3c clades, in contrast to the PCV2-positive cases detected in domestic pigs in 2023 that were classified in the PCV2d genotype. Conversely, samples from wild boars belong to the PCV2d and PCV3a clades. These results reveal genotype differences between wild and domestic pigs and shifts from 2023 to 2024. Our findings provide some information about the circulation of these viruses and emphasize the importance of vaccination and continued monitoring for a deeper understanding of their epidemiology to mitigate potential risks to swine health and production. Full article

A company’s competitive advantage largely depends on the longevity and quality of its customer relationships, making it essential to understand which tools best support these interactions. In particular, identifying the factors that shape the impact of Customer Relationship Management (CRM) systems on business performance is crucial. This study examines the influence of CRM on the business performance of Portuguese companies by employing a conceptual model structured around five dimensions: customer-centric management (CCM), CRM organization (CRMO), operational CRM (OCRM), customer service quality (CSQ), and technological turbulence (TT). Data were gathered via a questionnaire completed by employees of Portuguese firms using CRM systems, yielding a total of 228 valid responses. Of the nine hypotheses tested, eight were confirmed. The results indicate that CRM organization (CRMO) exerts the strongest positive influence on business performance (0.457), followed by customer service quality (CSQ), operational CRM (OCRM), and customer-centric management (CCM). The study also confirms that technological turbulence (TT) moderates the relationship between the CRM dimensions and business performance. These findings suggest that the proposed model is well-suited to the context of Portuguese SMEs and provide valuable insights for managers aiming to enhance competitiveness through the strategic use of CRM systems. Additionally, the results offer a relevant contribution to the academic literature on CRM and business performance. Full article

Transthyretin (TTR) variant (V30M) polyneuropathy (ATTRv-PN) is a progressive systemic amyloidosis caused by transthyretin aggregation, leading to a variety of debilitating manifestations, including neuropathy and cardiomyopathy. We investigated the plasma proteome of heterozygotic V30M TTR asymptomatic carriers and heterozygotic V30M ATTRv-PN patients (before and after tafamidis treatment) versus WT TTR healthy control plasma using an organic solvent-induced shift in solubility assay to identify biosignatures for disease progression and therapeutic response. We identified many proteins, including TTR, apolipoproteins, ceruloplasmin, and proteins with functions in innate immunity that displayed changes in either their abundances or their sensitivity to precipitation. Elevated oxidative modifications of TTR and APOE in ATTRv-PN patients suggest a role for oxidative stress in disease pathogenesis/progression. Tafamidis treatment mitigated these pathology-associated changes, suggesting that alleviating proteotoxic stress impacts these other pathways. Although our study was limited to a Portuguese cohort, these findings nevertheless provide a comprehensive plasma proteomic profile of V30M ATTRv-PN patients, V30M TTR carriers, and tafamidis-treated ATTRv-PN patients over up to 60 months; provide insights into ATTRv-PN pathophysiology; identify potential biomarkers for disease progression and therapeutic response; and highlight the utility of proteomics in advancing personalized treatments for amyloidosis. Full article

The present contribution addresses the Heat Waves (HWs) which occurred in Portugal’s mainland during the first 24 years of the XXI century: the number of HWs, their frequency, duration and geographic localization, among other impacts, are described. In a complementary perspective, due to the significant impacts of the 2003 HW, specifically in terms of mortality, a more detailed analysis of this event is performed. For the present analysis, HWs were identified using a modified version of the Heat Wave Duration Index (HWDI) proposed by the World Meteorological Organization (WMO). During the XXI century, between 2001 and 2024, 95 HWs occurred in the Portuguese mainland. In 2024, eight HWs occurred, followed by 2009, 2015 and 2017, with seven HWs each; in terms of monthly distribution, August (17) and May (16) displayed the highest values. HWs are now included in the World Health Organization agenda of natural hazards, enhancing the importance of these events. It is time to start considering HWs and their significant impacts as an important issue, especially in countries with older populations, like Portugal. Full article

Genome editing technologies have revolutionized the production of microorganisms, plants, and animals with phenotypes of interest to agriculture. Editing previously sequenced genomes allows for the punctual, discreet, precise, and accurate alteration of DNA for genetic analysis, genotyping, and phenotyping, as well as the production of edited organisms for academic and industrial purposes, among many other objectives. In this context, genome editing technologies have been causing a revolution in Brazilian agriculture. Thanks to the publication of Normative Resolution No. 16 (in Portuguese Resolução Normativa No. 16-RN16) in 2018, Brazilian regulatory authorities have adapted to the new genetic manipulation technologies available to the scientific community. This review aims to describe the effects of updates to the regulatory framework for edited organisms in Brazil and to point out their impacts on research and development of emerging technologies in the Brazilian agricultural sector. The implementation of RN16 rationalized the regulatory aspects regarding the production, manipulation, exploration and commercial release of edited organisms and led to the faster, cheaper and safer obtaining of edited technologies, which are more productive and better adapted to different environmental conditions in Brazil. Full article

Introduction: Visual skills have a significant impact on an infant’s development and participation. In Portugal, although the public health system is organized to support citizens with visual impairment, it lacks translated and culturally adapted assessment tools. Knowing that a comprehensive assessment is crucial for early diagnosis and that it improves an infant’s participation in daily life, the aim of the present article is to present the translation and cultural adaptation of the Participation and Activity Inventory for Children and Youth (0–2 years) into European Portuguese. Methods: To achieve the objective, methodological guidelines were followed, and six different stages were implemented, including translation, synthesis, back translation, revision by an expert committee, pretesting in a representative sample, and submission and appraisal of all written reports by developers to the author’s tool. The whole process was previously approved by an Ethics Committee. Results: A Portuguese version of the Participation and Activity Inventory for Children and Youth (0–2 years) was obtained based on the English version of PAI-CY (0–2 years) with the participation of four independent translators, five experts, and twenty parents filling out the pretest version. The results of the expert committee showed that 99.40% were in favor of the proposed translation. Additionally, the inventory pretesting reveals a clarity PAI-CY validity index of 1, a relevance PAI-CY validity index of 0.98, and a global validity index of 0.99. Conclusions: The Participation and Activity Inventory for Children and Youth (0–2 years) has now been translated and culturally adapted into European Portuguese, guaranteeing its content validity. To use the tool in Portugal with certainty it is necessary to implement the tool validation. The process to investigate the psychometric properties of the questionnaire is being prepared. Full article

Portuguese farmers seek evidence that organic fertilisers, particularly manure-based ones, can be safely used as partial replacements for mineral fertilisers (MFs), taking advantage of their nutrient and organic matter (OM) content. This study aimed to clarify the effects of applying organic fertilisers, especially under no-till practices in maize production. The experiment involved replacing basal mineral nitrogen (N) fertilisation with composted animal manure (CM) and pig slurry (PS) across three contrasting fields with varying soil characteristics, tillage techniques, and OM supplementation. Results indicated that site 1, which had the lowest clay and OM content, benefited the most from organic fertilisers, particularly in enhanced nutrient absorption in both maize leaves and grain. In this site, partial nutrient balance of N was significantly higher in the PS treatment (0.56 kg grain N exported kg N applied⁻¹) compared with the treatment with only MF (0.44). The impacts on greenhouse gas (GHG) emissions depended on site characteristics. CM led to higher emissions at site 2 (CH₄) and site 3 (N₂O), while PS did not increase GHG emissions at any site. Under no-till practices at site 3, CM resulted in higher global warming potential (154 kg CO_2-eq ha⁻¹) compared with the PS (128 kg CO_2-eq ha⁻¹) and MF (109 kg CO_2-eq ha⁻¹) treatments. Overall, this study suggests that organic fertilisers, particularly PS, can be a viable strategy for improving soil health and maintaining sustainable maize production in Portuguese agriculture. However, their effectiveness depends on factors such as soil texture, OM content, and tillage practices. In soils with lower OM content or under no-till practices, pig slurry emerges as a suitable alternative, replacing 30% of total mineral N fertilisation without compromising productivity or increasing GHG emissions. Full article

Objective: This study aimed to review the structural concepts, definition, classification, and macronutrient and food composition of carbohydrate-restricted diets (CRDs) for individuals with type 2 diabetes mellitus (T2DM). Methods: A scoping review was conducted following Joanna Briggs Institute guidelines. Searches were performed in Scopus, PubMed, Web of Science, and Embase, including texts published in Portuguese, English, and Spanish. Official documents from governments, regulatory agencies, and international diabetes organizations were also consulted. Results: In total, 79 articles and 17 official documents were analyzed. The following structural concept was identified: restricted carbohydrate intake decreases the need for endogenous and exogenous insulin, contributing to the maintenance of glycemic control, and justifies its consideration among the nutritional therapy options for individuals with T2DM. CRDs varied in definition, classification, and macronutrient composition. Studies failed to provide detailed information on the food composition of diets, precluding an in-depth understanding of metabolic effects. The existence of several approaches with varying recommendations makes it difficult to generalize the results. International CRD guidelines for T2DM adopt divergent definitions, compromising interpretation, recommendation, and even adherence. Conclusions: Although the concept of CRDs justifies their adoption within the nutritional therapy choices for T2DM, the multiple denominations can hinder understanding and comparison between studies. The lack of information on food composition and carbohydrate types compromises the assessment of the effects and adherence to CRD-based nutritional interventions. We emphasize the need for methodologically consistent studies that evaluate CRDs based on fresh and minimally processed foods with a low glycemic index to support official diabetes guidelines and organizations. Full article

Background: Vulnerability to stress is associated with susceptibility to react to certain events, taking into account individual factors, beliefs, and available resources. Workplace bullying increases stress, absenteeism, and turnover among nurses, creating a toxic environment. This negatively impacts their well-being and compromises the quality of nursing care. As a result, patient safety and healthcare outcomes may be affected. Objectives: To assess the prevalence of vulnerability to stress in Portuguese nurses and its relationship with the experience of workplace bullying. Methodology: This study used a descriptive–correlational approach with a cross-sectional design. The data collection instrument used was a questionnaire consisting of a sociodemographic component and a scale assessing vulnerability to stress (QVS-23) and workplace bullying (NAQ-R). The total sample consisted of 2015 nurses working in healthcare institutions. Results: Nurses who perceived themselves as victims of workplace bullying were shown to be more vulnerable to stress, with a higher mean value for perfectionism and dramatization of existence, inhibition and functional dependence, lack of support and deprivation of affection, intolerance of frustration and rejection, and adverse living conditions. Conclusions: Organizations must create a healthy, respectful, and productive working environment by identifying the various factors that make them vulnerable to stress in the workplace. They must also act to prevent workplace bullying, thus protecting the health of nurses and promoting positive relationships and a harmonious work culture. Full article

This article explores the ecological role of strawberry tree forests (Arbutus unedo L.) in the resilience of Portuguese forest ecosystems and their relationship with plant production as a source of food. It discusses the importance of the strawberry tree in fire combat and the improvement of agroforestry areas by mitigating erosion and augmenting soil organic matter. The multifunctionality of their fruits, emphasizing their utilization in food and beverage production, is also addressed. Moreover, the socio-economic and cultural significance of fruit production, emphasizing its role in sustainable development, is analyzed. The diversity of beverages (spirits, liquors, and gin) and food products (jams, jellies, etc.) effectively contribute to have a positive social and economic impact on the local populations and tradition maintenance. Moreover, the bioactive compounds in different parts of the plants and fruits have applications in pharmacology and cosmetics. Finally, the valorization of strawberry tree spirits mush waste as a source of natural dyes for textiles is discussed as a promising research topic to be explored in the future. Full article

In the modern workplace, understanding the dynamics of work passion and its consequences on employee well-being is crucial. This quantitative study, conducted with Portuguese workers, seeks to examine and validate the Dualistic Model of Passion, which distinguishes between harmonious and obsessive passion, while also exploring the intricate relationships among work passion, job satisfaction, work–family conflict, and burnout. Using mediation analyses and structural equation modeling (SEM), a convenience sample of 326 participants (169 men and 157 women), aged between 19 and 69 years (M = 41.31, SD = 10.52), was analyzed to uncover how different types of work passion—obsessive and harmonious—impact professional outcomes and well-being. The findings reveal that obsessive passion for work is a significant predictor of work–family conflict, which, in turn, escalates the risk of burnout. In contrast, harmonious passion is positively associated with increased job satisfaction and acts as a buffer against the negative effects of work–family conflict. Notably, contrary to initial assumptions, obsessive passion does not directly precipitate burnout. Mediation analyses confirmed that work–family conflict plays a key role in this process, while SEM results demonstrated strong model fit indices, reinforcing its relevance and applicability in organizational settings. These results offer crucial insights for both theoretical advancement and practical applications, highlighting the importance of fostering a work environment that nurtures harmonious passion. By doing so, organizations can mitigate conflict and burnout while promoting higher levels of job satisfaction among employees. Full article

Farmers are an important and fundamental professional class on which the food, health and well-being of all citizens depend. Recently, and similar to what has happened in other European countries, in Portugal there were also demonstrations of civic and spontaneous movements of farmers dissatisfied with the current Common Agricultural Policy Strategic Plan (PEPAC), with the excessive bureaucracy and with the increase in the costs of production and unfair competition from agricultural products from other countries, especially from outside the European bloc, which do not meet the same production requirements and which are available to consumers. This work consists of a synthesis and reflection on the reasons, arguments and demands made by civic movements of farmers in the northern region of Portugal (NUT II). Among the most frequent allegations was the need to consider specific aspects of the northern Portuguese region, small family farming and some specific sectors. Although the factor that triggered farmers’ indignation was the reduction in support payable under eco-schemes, the consideration of the common land areas for subsidies and for certification in organic farming (in the mountain region), the installation of anti-hail towers in the fruit growing area and compensation for increases in energy and animal feeding costs are among the most frequently mentioned reasons for which farmers require answers. Full article

Soil carbon sequestration (SCS) is a nature-based, low-cost climate mitigation strategy that also contributes to the climate adaptation of agricultural systems. Some land-use and land-management practices potentially lead to an enhancement of the soil organic carbon (SOC) sink, such as no-till, the use of cover crops, leaving residues on fields, improving the variety of legume species in grasslands and reducing grazing intensity. However, uncertainties remain both in estimating and measuring the impact of the application of certain practices, as these vary with the soil, climate and historic land use. IPCC (Intergovernmental Panel on Climate Change) guidelines are commonly used to estimate SOC and SOC sequestration potentials at different tiers. Here, the IPCC’s tier 1 methodology was applied to estimate (1) the sequestration potential of nine mitigation practices and (2) the emission or sequestration potential of four current land-change trends for n = 7092 unique agricultural sites in mainland Portugal. The conversion of irrigated crops to improved grasslands resulted in the highest average unit sequestration (1.05 tC ha⁻¹ yr⁻¹), while cropland conversion to poor degraded pasture (abandonment) resulted in the highest unit SOC loss (−0.08 tC ha⁻¹ yr⁻¹). The abandonment of cropland results in a national SOC loss of up to 0.09 MtC yr⁻¹, while the improvement of poor degraded pastures has the highest national sequestration potential, equal to 0.6 MtC yr⁻¹ (2.2 MtCO_2eq yr⁻¹), about 4% of Portugal’s emissions in 2021, if applied in all managed areas. The results enable a comparison between different practices and land uses; however, to enhance accuracy, a higher tier methodology tailored to the Portuguese context should be developed. Full article

The lack of evidence synthesis studies on the role of psychiatric mental health nursing in the context of psychiatric home hospitalization underscores the limited research on nurses providing care in these teams, particularly concerning their experiences and responsibilities and the actual role of nurses in this context. This knowledge has the potential to improve the quality of mental healthcare by guiding nursing practices. Objective: To map the concepts related to the role of mental health nurses in psychiatric home hospitalization. Methods: A scoping review was conducted using the methodology proposed by the JBI, involving five methodological stages. No search limits were applied except for language. Results: The review identified five key dimensions of nursing in psychiatric home hospitalization: satisfaction; care models; the therapeutic relationship; the care environment; the organization of care. These interconnected dimensions influence quality care. Satisfaction among nurses, patients, and families is associated with improved outcomes and reduced stigma. The therapeutic relationship is characterized by a humanistic approach, emphasizing dialogue, empathy, and shared decision making. Additionally, the importance of care organization is highlighted, including individualized care plans, medication management, and intersectoral collaboration. Discussion: The five nursing dimensions align with the Quality Standards of the Portuguese Nursing Council and are consistent with the scientific literature. Conclusions: Although there is a shortage of studies on this topic, this review allows for the synthesis of nursing interventions and reflection on the paradigm shift in care within the scope of psychiatric mental health nursing interventions. Future studies highlighting the value of mental health nursing interventions, with a particular focus on nursing-sensitive indicators and employing quantitative or mixed methods, will be crucial to furthering the analysis conducted thus far. Full article

The integration of artificial intelligence (AI) in the public sector is driving significant advancements in governance and management, changing the way public organizations operate. In particular, AI technologies have a profound impact on auditing practices, enhancing efficiency and accountability. This article aims to explore how AI can improve audit processes in a Portuguese public security agency, focusing on its transformative potential in streamlining tasks such as data extraction, analysis, and verification. Using a qualitative research approach, the study employs custom Python algorithms to examine the integration of key indicators into the audit process, specifically through the analysis of economic classification and expenditure limits. The findings demonstrate that personalized algorithms can reduce manual workloads, improve accuracy, and strengthen compliance with financial regulations, providing valuable contributions for decision-making. However, challenges such as data privacy and infrastructure investment remain, emphasizing the need for further research. Future studies should focus on adapting AI-based auditing models to various public administration contexts, addressing organizational changes, and advancing public governance. Full article