Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (90)

Search Parameters:
Keywords = Populus tomentosa

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
16 pages, 3549 KiB  
Article
Functional Characterization of PtoWOX1 in Regulating Leaf Morphogenesis and Photosynthesis in Populus tomentosa
by Feng Tang, Minghui He, Shi Liang, Meng Zhang, Xiaowei Guo, Yuxian Dou, Qin Song, Cunfeng Zhao and Ting Lan
Plants 2025, 14(14), 2138; https://doi.org/10.3390/plants14142138 - 10 Jul 2025
Viewed by 301
Abstract
Leaves are essential for photosynthesis and transpiration, directly influencing plant growth and development. Leaf morphology, such as length, width, and area, affects photosynthetic efficiency and transpiration rates. In this study, we investigated the role of PtoWOX1 in leaf morphogenesis by generating both overexpression [...] Read more.
Leaves are essential for photosynthesis and transpiration, directly influencing plant growth and development. Leaf morphology, such as length, width, and area, affects photosynthetic efficiency and transpiration rates. In this study, we investigated the role of PtoWOX1 in leaf morphogenesis by generating both overexpression and CRISPR/Cas9 knockout lines in P. tomentosa. The results showed that PtoWOX1A and PtoWOX1B encode nuclear-localized transcription factors highly expressed in young leaves, particularly in palisade and epidermal cells. Knockout of PtoWOX1 resulted in reduced leaf width and area, enlarged upper epidermal cells, and lower stomatal density. Overexpression led to wrinkled leaf surfaces and reduced margin serration. Anatomical analysis revealed altered palisade cell arrangement and increased leaf thickness in knockout lines, accompanied by higher chlorophyll content and enhanced photosynthetic rates. Additionally, PtoWOX1A interacts with PtoYAB3B, suggesting a complex that regulates leaf margin development. These findings clarify the function of PtoWOX1 in regulating mid-lateral axis development and leaf margin morphology and provide new insights for the molecular breeding of poplar. Full article
Show Figures

Figure 1

19 pages, 3977 KiB  
Article
Genome-Wide Analysis of the CDPK Gene Family in Populus tomentosa and Their Expressions in Response to Arsenic Stress and Arbuscular Mycorrhizal Fungi Colonization
by Minggui Gong, Jiajie Su, Shuaihui Wang, Youjia Wang, Weipeng Wang, Xuedong Chen and Qiaoming Zhang
Agronomy 2025, 15(7), 1655; https://doi.org/10.3390/agronomy15071655 - 8 Jul 2025
Viewed by 356
Abstract
Calcium-dependent protein kinases (CDPKs) are crucial regulators in calcium-mediated signal transduction pathways, playing a pivotal role in plant response to abiotic stresses. However, there is still limited knowledge regarding the genes of the Populus tomentosa CDPK family and their underlying functions in response [...] Read more.
Calcium-dependent protein kinases (CDPKs) are crucial regulators in calcium-mediated signal transduction pathways, playing a pivotal role in plant response to abiotic stresses. However, there is still limited knowledge regarding the genes of the Populus tomentosa CDPK family and their underlying functions in response to arsenic (As) stress and arbuscular mycorrhizal fungi (AMF) colonization. In our study, 20 PtCDPKs were identified in the P. tomentosa genome. Phylogenetic analysis categorized these PtCDPK genes into four subgroups based on sequence homology. Motif analysis revealed that PtCDPK genes within the same group share a similar exon–intron structure, conserved domains, and composition. The promoters of PtCDPK genes were found to contain a multitude of cis-acting elements, including light-response elements, phytohormone-response elements, and stress-response elements. The analysis of genes provided insights into the evolutionary dynamics and expansion of the PtCDPK gene family within P. tomentosa. The PtCDPK genes exhibited a strong collinear relationship with the CDPK genes of two model plants, namely, Arabidopsis thaliana and Oryza sativa L. Specifically, 10 gene pairs showed collinearity with Arabidopsis; in contrast, 14 gene pairs were collinear with rice. Transcriptome analysis of gene expression levels in P. tomentosa roots under both As stress and arbuscular mycorrhizal fungi (AMF) colonization conditions revealed that 20 PtCDPK genes had differential expression patterns. Under As stress, AMF inoculation led to the upregulation of 11 PtCDPK genes (PtCDPKSK5, X2, 1-3, 20-1, 24, 26-X1-1, 26-X1-2, 29-1, 29-2, 32, and 32-X1) and the downregulation of 8 PtCDPK genes, including PtCDPK1-1, 1-2, 8-X1, 10-X4, 13, 20-2, 26-X2, and 26-X3. The RT-qPCR results for 10 PtCDPK genes were consistent with the transcriptome data, indicating that AMF symbiosis plays a regulatory role in modulating the expression of PtCDPK genes in response to As stress. The principal findings of this study were that PtCDPK genes showed differential expression patterns under As stress and AMF colonization, with AMF regulating PtCDPK gene expression in response to As stress. Our study contributes to developing a deeper understanding of the function of PtCDPKs in the Ca2+ signaling pathway of P. tomentosa under As stress and AMF inoculation, which is pivotal for elucidating the molecular mechanisms underlying As tolerance in AMF-inoculated P. tomentosa. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
Show Figures

Figure 1

21 pages, 4818 KiB  
Article
Typical Greening Species Based on Five “Capability Indicators” Under the Artificial Control of Negative Ion Releasing Capacity
by Shaoning Li, Di Yu, Na Zhao, Tingting Li, Bin Li, Xiaotian Xu and Shaowei Lu
Forests 2025, 16(7), 1037; https://doi.org/10.3390/f16071037 - 20 Jun 2025
Viewed by 247
Abstract
Negative air ions (NAIs) can purify the atmosphere and maintain human health. In this study, we selected six tree species, Pinus tabuliformis, Pinus bungeana, Acer truncatum, Sophora japonica, Koelreuteria paniculata, Quercus variabilis, Robinia pseudoacacia, and Populus [...] Read more.
Negative air ions (NAIs) can purify the atmosphere and maintain human health. In this study, we selected six tree species, Pinus tabuliformis, Pinus bungeana, Acer truncatum, Sophora japonica, Koelreuteria paniculata, Quercus variabilis, Robinia pseudoacacia, and Populus tomentosa, and we established for the first time five “capacity indicators” to characterize and judge the capacity of plants to release negative ions: they comprised the release contribution rate L, release coefficient n, release rate s, instantaneous current number v, and total level of release Z. These indicators were used to assess the ability of the plants to release NAIs by themselves. The results showed the following. (1) The daily variations in L and n show “W” and “concave” shapes, respectively, and the contribution capacity at night is significantly higher than that during the day. The diurnal variations in s, v, and Z all showed a “bimodal” pattern. The NAI release rate and release level of each tree species during the day were significantly higher than those at night. (2) The trees released the most NAIs during the day at approximately 10:00, while Robinia pseudoacacia and Populus tomentosa peaked with a 2 h lag (12:00). The NAI release capacity of each tree species was the worst at 13:00. (3) During the growing season, the self-contribution effects L and n of the plants were the strongest in May. The release rates and release levels s, v, and Z were the lowest in August. The coniferous plants released NAIs at the fastest rate in September and broad-leaved plants in July, with the highest release levels. In this study, the plants released the most NAIs from 10:00 a.m. to 11:00 a.m., which is the best time to travel. Quercus variabilis was preferentially recommended in the pairing of species of tree with the quickest NAI release and the highest total number released, followed by Koelreuteria paniculata and Sophora japonica. Full article
(This article belongs to the Section Forest Meteorology and Climate Change)
Show Figures

Figure 1

19 pages, 2474 KiB  
Article
Growth and Biomass Distribution Responses of Populus tomentosa to Long-Term Water–Nitrogen Coupling in the North China Plain
by Yafei Wang, Juntao Liu, Yuelin He, Wei Zhu, Liming Jia and Benye Xi
Plants 2025, 14(12), 1833; https://doi.org/10.3390/plants14121833 - 14 Jun 2025
Viewed by 435
Abstract
From 2016 to 2021, a field experiment was conducted in the North China Plain to study the long-term effects of drip irrigation and nitrogen coupling on the growth, biomass allocation, and irrigation water and fertilizer use efficiency of short-rotation triploid Populus tomentosa plantations. [...] Read more.
From 2016 to 2021, a field experiment was conducted in the North China Plain to study the long-term effects of drip irrigation and nitrogen coupling on the growth, biomass allocation, and irrigation water and fertilizer use efficiency of short-rotation triploid Populus tomentosa plantations. The experiment adopted a completely randomized block design, with one control (CK) and six water–nitrogen coupling treatments (IF, two irrigation levels × three nitrogen application levels). Data analysis was conducted using ANOVA, regression models, Spearman’s correlation analysis, and path analysis. The results showed that the effects of water and nitrogen treatments on the annual increment of diameter at breast height (ΔDBH), annual increment of tree height (ΔH), basal area of the stand (BAS), stand volume (VS), and annual forest productivity (AFP) in short-rotation forestry exhibited a significant stand age effect. The coupling of water and nitrogen significantly promoted the DBH growth of 2-year-old trees (p < 0.05), but after 3 years of age, the promoting effect of water and nitrogen coupling gradually diminished. In the 6th year, the above-ground biomass of Populus tomentosa was 5.16 to 6.62 times the under-ground biomass under different treatments. Compared to the I45 treatment (irrigation at soil water potential of −45 kPa), the irrigation water use efficiency of the I20 treatment (−20 kPa) decreased by 88.79%. PFP showed a downward trend with the increase in fertilization amount, dropping by 130.95% and 132.86% under the I20 and I45 irrigation levels. Path analysis indicated that irrigation had a significant effect on the BAS, VS, AFP, and TGB of 6-year-old Populus tomentosa (p < 0.05), with the universality of irrigation being higher than that of fertilization. It is recommended to implement phased water and fertilizer management for Populus tomentosa plantations in the North China Plain. During 1–3 years of tree age, adequate irrigation should be ensured and nitrogen fertilizer application increased. Between the ages of 4 and 6, irrigation and fertilization should be ceased to reduce resource wastage. This work provides scientific guidance for water and fertilizer management in short-rotation plantations. Full article
Show Figures

Figure 1

13 pages, 4156 KiB  
Article
Plant Functional Traits and Soil Nutrients Drive Divergent Symbiotic Fungal Strategies in Three Urban Street Tree Species
by Yifan Xue, Yao Wang, Jiang Shi, Jingyao Wei, Qiong Wang and Wenchen Song
J. Fungi 2025, 11(6), 454; https://doi.org/10.3390/jof11060454 - 14 Jun 2025
Viewed by 557
Abstract
Understanding species-specific mechanisms governing symbiotic fungal responses to plant traits and soil factors is critical for optimizing urban tree “plant-soil-fungus” systems under pollution stress. To address this gap, we combined δ13C/δ15N isotope analysis and ITS sequencing for three common [...] Read more.
Understanding species-specific mechanisms governing symbiotic fungal responses to plant traits and soil factors is critical for optimizing urban tree “plant-soil-fungus” systems under pollution stress. To address this gap, we combined δ13C/δ15N isotope analysis and ITS sequencing for three common street trees in Beijing: Sophora japonica, Ginkgo biloba, and Populus tomentosa. In S. japonica, symbiotic fungal abundance was positively associated with leaf δ15N, indicating root exudate-mediated “plant-microbe” interactions during atmospheric NOx assimilation. G. biloba, with weak NOx assimilation, exhibited a negative correlation between fungal abundance and soil available N/P, suggesting mycorrhizal nutrient compensation under low fertility. P. tomentosa showed decreased fungal abundance with increasing soil N/P ratios and specific leaf area, reflecting carbon allocation trade-offs that limit mycorrhizal investment. These results demonstrate that symbiotic fungi respond to atmospheric and edaphic drivers in a tree species-dependent manner. Urban greening strategies should prioritize S. japonica for its NOx mitigation potential and optimize fertilization for G. biloba (nutrient-sensitive fungi) and P. tomentosa (nutrient balance sensitivity). Strategic mixed planting of P. tomentosa with S. japonica could synergistically enhance ecosystem services through complementary resource acquisition patterns. This study provides mechanism-based strategies for optimizing urban tree management under atmospheric pollution stress. Full article
Show Figures

Figure 1

14 pages, 4930 KiB  
Article
The Fiber Cell-Specific Overexpression of COMT2 Modulates Secondary Cell Wall Biosynthesis in Poplar
by Hanyu Chen, Hong Wang, Zhengjie Zhao, Jiarui Pan, Yao Yao, Yihan Wang, Keming Luo and Qin Song
Plants 2025, 14(12), 1739; https://doi.org/10.3390/plants14121739 - 6 Jun 2025
Viewed by 450
Abstract
Wood, as a natural and renewable resource, plays a crucial role in industrial production and daily life. Lignin, as one of the three major components of the plant cell secondary wall, plays a key role in conferring mechanical strength and enhancing stress resistance. [...] Read more.
Wood, as a natural and renewable resource, plays a crucial role in industrial production and daily life. Lignin, as one of the three major components of the plant cell secondary wall, plays a key role in conferring mechanical strength and enhancing stress resistance. The caffeic acid-O-methyltransferase (COMT) family of oxygen-methyltransferases is a core regulatory node in the downstream pathway of lignin biosynthesis. Here, our report shows that caffeic acid-O-methyltransferase 2 (COMT2) exhibits high conservation across several species. Tissue expression analysis reveals that COMT2 is specifically highly expressed in the secondary xylem of Populus tomentosa stems. We demonstrated that the specific overexpression of COMT2 in fiber cells of Populus tomentosa led to a significant increase in plant height, stem diameter, internode number, and stem dry weight. Furthermore, we found that the specific overexpression of COMT2 in fiber cells promotes xylem differentiation, lignin accumulation, and the thickening of the secondary cell wall (SCW) in fiber cells. Our results indicate that key downstream lignin biosynthesis enzyme genes are upregulated in transgenic plants. Additionally, mechanical properties of stem bending resistance, puncture resistance, and compressive strength in the transgenic lines are significantly improved. Moreover, we further created the DUFpro:COMT2 transgenic lines of Populus deltoides × Populus. euramericana cv ‘Nanlin895’ to verify the functional conservation of COMT2 in closely related poplar species. The DUFpro:COMT2 Populus deltoides × Populus. euramericana cv ‘Nanlin895’ transgenic lines exhibited phenotypes similar to those observed in the P. tomentosa transgenic plants, which showed enhanced growth, increased lignin accumulation, and greater wood strength. Overall, the specific overexpression of the caffeic acid O-methyltransferase gene COMT2 in poplar stem fiber cells has enhanced the wood biomass, wood properties, and mechanical strength of poplar stems. Full article
Show Figures

Figure 1

18 pages, 9557 KiB  
Article
Cell Wall Invertase 4 Governs Sucrose–Hexose Homeostasis in the Apoplast to Regulate Wood Development in Poplar
by Jing Lu, Qiao Ren, Qilin Wang, Yaqi Wen, Yanhong Wang, Ruiqi Liang, Dingxin Ran, Yifeng Jia, Xinyu Zhuo, Jiangtao Luo, Xianqiang Wang and Keming Luo
Plants 2025, 14(9), 1388; https://doi.org/10.3390/plants14091388 - 4 May 2025
Viewed by 584
Abstract
In perennial trees, wood development is a carbon-demanding process, pivotal for secondary cell wall (SCW) formation and xylem development. Sugars, functioning both as carbon substrates and signaling molecules, orchestrate cambial proliferation and xylem differentiation. However, few molecular candidates involved in the sugar-mediated regulation [...] Read more.
In perennial trees, wood development is a carbon-demanding process, pivotal for secondary cell wall (SCW) formation and xylem development. Sugars, functioning both as carbon substrates and signaling molecules, orchestrate cambial proliferation and xylem differentiation. However, few molecular candidates involved in the sugar-mediated regulation of wood development have been characterized. Cell wall invertases (CWINs), a subclass of the invertase enzyme family localized in the apoplastic space, catalyze the irreversible hydrolysis of sucrose into glucose and fructose, thereby governing carbon allocation in sink tissues. Here, PtoCWIN4 shows preferential expression in the stem of Populus tomentosa and has a high efficiency in sucrose cleavage activity. We demonstrated that the knockout of PtoCWIN4 results in stunted growth, aberrant branching patterns, and compromised secondary xylem formation. In contrast, mutant lines displayed enhanced SCW thickness accompanied by elevated cellulose and hemicellulose accumulation. Following this, the knockout of PtoCWIN4 led to impaired carbon partitioning from sucrose to hexose metabolites during wood development, corroborating the enzyme’s role in sustaining sucrose hydrolysis. Collectively, these findings establish PtoCWIN4 as a master regulator of sucrose-to-hexose conversion, a metabolic gateway critical for balancing structural biomass production and developmental growth during wood formation. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
Show Figures

Figure 1

16 pages, 2320 KiB  
Article
Transposon-Associated Small RNAs Involved in Plant Defense in Poplar
by Cui Long, Yuxin Du, Yumeng Guan, Sijia Liu and Jianbo Xie
Plants 2025, 14(8), 1265; https://doi.org/10.3390/plants14081265 - 21 Apr 2025
Viewed by 534
Abstract
Utilizing high-throughput Illumina sequencing, we examined how small RNA (sRNA) profiles vary in Chinese white poplar (Populus tomentosa) across two pivotal infection stages by the rust fungus Melampsora larici-populina: the biotrophic growth phase (T02; 48 h post infection) and the [...] Read more.
Utilizing high-throughput Illumina sequencing, we examined how small RNA (sRNA) profiles vary in Chinese white poplar (Populus tomentosa) across two pivotal infection stages by the rust fungus Melampsora larici-populina: the biotrophic growth phase (T02; 48 h post infection) and the urediniospore development and dispersal phase (T03; 168 h), both essential for plant colonization and prolonged biotrophic engagement. Far exceeding random expectations, siRNA clusters predominantly arose from transposon regions, with pseudogenes also contributing significantly, and infection-stage-specific variations were notably tied to these transposon-derived siRNAs. As the infection advanced, clusters of 24 nt siRNAs in transposon and intergenic regions exhibited pronounced abundance shifts. An analysis of targets indicated that Populus sRNAs potentially regulate 95% of Melampsora larici-populina genes, with pathogen effector genes showing heightened targeting by sRNAs during the biotrophic and urediniospore phases compared to controls, pointing to selective sRNA-target interactions. In contrast to conserved miRNAs across plant species, Populus-specific miRNAs displayed a markedly greater tendency to target NB-LRR genes. These observations collectively highlight the innovative roles of sRNAs in plant defense, their evolutionary roots, and their dynamic interplay with pathogen coevolution. Full article
(This article belongs to the Special Issue Genetic Breeding of Trees)
Show Figures

Figure 1

26 pages, 27617 KiB  
Article
MFCPopulus: A Point Cloud Completion Network Based on Multi-Feature Fusion for the 3D Reconstruction of Individual Populus Tomentosa in Planted Forests
by Hao Liu, Meng Yang, Benye Xi, Xin Wang, Qingqing Huang, Cong Xu and Weiliang Meng
Forests 2025, 16(4), 635; https://doi.org/10.3390/f16040635 - 5 Apr 2025
Viewed by 533
Abstract
The accurate point cloud completion of individual tree crowns is critical for quantifying crown complexity and advancing precision forestry, yet it remains challenging in dense plantations due to canopy occlusion and LiDAR limitations. In this study, we extended the scope of conventional point [...] Read more.
The accurate point cloud completion of individual tree crowns is critical for quantifying crown complexity and advancing precision forestry, yet it remains challenging in dense plantations due to canopy occlusion and LiDAR limitations. In this study, we extended the scope of conventional point cloud completion techniques to artificial planted forests by introducing a novel approach called Multi−feature Fusion Completion of Populus (MFCPopulus). Specifically designed for Populus Tomentosa plantations with uniform spacing, this method utilized a dataset of 1050 manually segmented trees with expert−validated trunk−canopy separation. Key innovations include the following: (1) a hierarchical adversarial framework that integrates multi−scale feature extraction (via Farthest Point Sampling at varying rates) and biologically informed normalization to address trunk−canopy density disparities; (2) a structural characteristics split−collocation (SCS−SCC) strategy that prioritizes crown reconstruction through adaptive sampling ratios, achieving a 94.5% canopy coverage in outputs; (3) a cross−layer feature integration enabling the simultaneous recovery of global contours and a fine−grained branch topology. Compared to state−of−the−art methods, MFCPopulus reduced the Chamfer distance variance by 23% and structural complexity discrepancies (ΔDb) by 33% (mean, 0.12), while preserving species−specific morphological patterns. Octree analysis demonstrated an 89−94% spatial alignment with ground truth across height ratios (HR = 1.25−5.0). Although initially developed for artificial planted forests, the framework generalizes well to diverse species, accurately reconstructing 3D crown structures for both broadleaf (Fagus sylvatica, Acer campestre) and coniferous species (Pinus sylvestris) across public datasets, providing a precise and generalizable solution for cross−species trees’ phenotypic studies. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
Show Figures

Figure 1

20 pages, 4896 KiB  
Article
Multi-Criteria Plant Clustering for Carbon-Centric Urban Forestry: Enhancing Sequestration Potential Through Adaptive Species Selection in the Zhengzhou Metropolitan Area, China
by Qiutan Ren, Lingling Zhang, Zhilan Yang, Mengting Zhang, Mengqi Wei, Honglin Zhang, Ang Li, Rong Shi, Peihao Song and Shidong Ge
Forests 2025, 16(3), 536; https://doi.org/10.3390/f16030536 - 19 Mar 2025
Viewed by 508
Abstract
As global climate change and urban issues worsen, increasing carbon offsets is crucial, with urban plants playing a key role. However, research on assessing plant carbon sequestration (CSE) capacity at the regional scale, selecting urban plants, and optimizing CSE capacity-based scenarios is still [...] Read more.
As global climate change and urban issues worsen, increasing carbon offsets is crucial, with urban plants playing a key role. However, research on assessing plant carbon sequestration (CSE) capacity at the regional scale, selecting urban plants, and optimizing CSE capacity-based scenarios is still limited. A total of 272 plant species were surveyed in the nine cities of the Zhengzhou Metropolitan Area (ZMA). The i-Tree and biomass models estimated the average carbon storage (CS) density at 9.32 kg C m−2 and the CSE density at 0.55 kg C y−2 m−2 in the ZMA. The highest CS density (13.58 kg C m−2) was observed in Pingdingshan, while the lowest CSE density (0.36 kg C y−1 m−2) was observed in Xuchang. Hierarchical and cluster analyses identified plant species with balanced CSE capacity, adaptability, and ornamental value, such as Populus tomentosa Carr. and Salix babylonica L., as well as shrubs like Abelia biflora Turcz and Kerria japonica (L.) DC. Vegetation regeneration modeling indicated that CS could increase by 37%–41% along roads, 28%–43% in amenity areas, and 17%–30% near waterfronts over the next 50 years. These findings serve as a reference for urban regeneration and planning aimed at enhancing the carbon reduction potential of urban green spaces (UGS). Full article
(This article belongs to the Section Urban Forestry)
Show Figures

Figure 1

25 pages, 14640 KiB  
Article
Genome-Wide Identification and Functional Analysis of CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) in Three Populus Species
by Zheng Li, Feng-Xin Chen, Ming-Ming Li, Xian-Li Tang, Yu-Qi Liu, Meng-Bo Huang, Hao-Qiang Niu, Chao Liu, Hou-Ling Wang, Xin-Li Xia and Wei-Lun Yin
Int. J. Mol. Sci. 2025, 26(5), 1944; https://doi.org/10.3390/ijms26051944 - 24 Feb 2025
Viewed by 812
Abstract
Intercellular communication mediated by CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides and their receptors is crucial for plant development and environmental adaptation. In this study, 45 and 89 CLEs were identified in Populus tomentosa and Populus alba × Populus glandulosa, respectively, and, together with [...] Read more.
Intercellular communication mediated by CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides and their receptors is crucial for plant development and environmental adaptation. In this study, 45 and 89 CLEs were identified in Populus tomentosa and Populus alba × Populus glandulosa, respectively, and, together with the 52 CLEs in Populus trichocarpa, the chromosome localization, gene and protein characteristics, collinearity and gene duplication events, cis-acting regulatory elements in promoters and evolutionary relationships of CLEs in these three poplar species were analyzed. The CLEs of three poplar species were divided into four subfamilies. Among them, the CLEs in subfamilies I, II and IV were A-type CLEs, while those in subfamily III were B-type CLEs. During the evolutionary process of poplar, the selection pressure faced by whole-genome duplication or segmental duplication was purifying selection, and the duplication events led to the expansion of the CLE family in poplar. The exogenous addition of a certain concentration of poplar CLE13 peptides inhibits the root growth of Arabidopsis thaliana and poplar and simultaneously reduces the expression levels of ARFs and LBDs in the roots. In addition, drought stress induces the expression of PtrCLE13A. The overexpression of preCLE13A significantly enhances the osmotic and drought tolerance in Populus tomentosa. These results have provided valuable information for further research on the molecular mechanisms of CLE peptide signaling pathways in the woody model plant poplar regarding plant growth and stress resistance. Full article
(This article belongs to the Section Molecular Plant Sciences)
Show Figures

Figure 1

15 pages, 8837 KiB  
Article
Construction and Properties of Wood-Based Tannin–Iron-Complexed Photothermal Material Populus tomentosa Carr.@Fe-GA for Solar Seawater Desalination System
by Hongyan Zhu, Xinyu Li, Shijie Li, Ximing Wang, Yabo Ma, Jin Zhang, Yunpeng Ren and Jianguo Zhao
Materials 2025, 18(2), 393; https://doi.org/10.3390/ma18020393 - 16 Jan 2025
Viewed by 930
Abstract
Desalinating seawater is a crucial method for addressing the shortage of freshwater resources. High-efficiency, low-cost, and environmentally friendly desalination technologies are key issues that urgently need to be addressed. This work used Populus tomentosa Carr. as a matrix material and prepared Populus tomentosa [...] Read more.
Desalinating seawater is a crucial method for addressing the shortage of freshwater resources. High-efficiency, low-cost, and environmentally friendly desalination technologies are key issues that urgently need to be addressed. This work used Populus tomentosa Carr. as a matrix material and prepared Populus tomentosa Carr.@Fe-GA through a complexation reaction to enhance the water evaporation rate and photothermal conversion efficiency of seawater desalination. The concentration of the impregnation solution was further refined, and the bonding mechanism along with the thermal stability of the composite photothermal material was investigated, including an assessment of their photothermal conversion efficiency. The research results indicate that the evaporation rate of water in a 3.5% NaCl solution for Populus tomentosa Carr.@Fe-GA under light intensity conditions of one sun reached 1.72 kg·m−2·h−1, which was an increase of 44.5% compared to untreated Populus tomentosa Carr. It achieved a photothermal conversion efficiency of 95.1%, an improvement of 53.6% over untreated Populus tomentosa Carr., and maintained stability and high evaporation performance (95.4%) even after prolonged rinsing. This work realizes the functional utilization of seawater desalination with Populus tomentosa Carr. and offers a novel approach for the development and use of wood-derived photothermal material. Full article
Show Figures

Figure 1

19 pages, 7353 KiB  
Article
Evaluation of Anti-Fungal Activities of Environmentally Friendly Wood Preservative from Thermal-Induced Lignified Twigs
by Xinqi Gao, Yafang Lei, Teng Sun, Yuanze Ma, Hao Guan and Li Yan
Forests 2025, 16(1), 119; https://doi.org/10.3390/f16010119 - 10 Jan 2025
Viewed by 931
Abstract
Enhancing the decay resistance of Populus tomentosa wood through environmentally friendly methods is crucial for improving its durability and market competitiveness. Lignified twigs (LT), typically unsuitable as timber due to their small diameter, are rich in lignin, which degrades during thermal induction to [...] Read more.
Enhancing the decay resistance of Populus tomentosa wood through environmentally friendly methods is crucial for improving its durability and market competitiveness. Lignified twigs (LT), typically unsuitable as timber due to their small diameter, are rich in lignin, which degrades during thermal induction to produce antifungal organic compounds. In this context, the objective of this study was to develop a lignified twig preservative (LTP) by thermal induction from the LT of Actinidia chinensis var. Jinyang. The antifungal activity of LTP under varying thermal conditions was analyzed, along with its chemical composition. Enzyme activity, cell membrane integrity, and respiratory metabolism in fungi treated with LTP were examined to elucidate antifungal mechanisms. Additionally, the decay resistance of LTP-treated wood was evaluated. Results revealed that LTP produced under N2 at 220 °C exhibited robust antifungal activity against Trametes versicolor and Gloeophyllum trabeum, attributed to phenolic compounds such as syringaldehyde, syringone, vanillin, and vanillone. LTP inhibited fungal cellulases, hemicellulases, and ligninases by 30%–60%, disrupted cell membrane functionality, and suppressed respiratory metabolism. Poplar wood treated with LTP demonstrated significantly enhanced decay resistance (mass loss < 10%). This thermal-induced feedback pattern shows great potential for LT in wood preservation. Full article
Show Figures

Figure 1

16 pages, 3259 KiB  
Article
Elevated CO2 Shifts Photosynthetic Constraint from Stomatal to Biochemical Limitations During Induction in Populus tomentosa and Eucalyptus robusta
by Xianhui Tang, Jie Zhao, Jiayu Zhou, Qingchen Zhu, Xiyang Sheng and Chao Yue
Plants 2025, 14(1), 47; https://doi.org/10.3390/plants14010047 - 27 Dec 2024
Cited by 1 | Viewed by 913
Abstract
The relative impacts of biochemical and stomatal limitations on photosynthesis during photosynthetic induction have been well studied for diverse plants under ambient CO2 concentration (Ca). However, a knowledge gap remains regarding how the various photosynthetic components limit duction efficiency [...] Read more.
The relative impacts of biochemical and stomatal limitations on photosynthesis during photosynthetic induction have been well studied for diverse plants under ambient CO2 concentration (Ca). However, a knowledge gap remains regarding how the various photosynthetic components limit duction efficiency under elevated CO2. In this study, we experimentally investigated the influence of elevated CO2 (from 400 to 800 μmol mol–1) on photosynthetic induction dynamics and its associated limitation components in two broadleaved tree species, Populus tomentosa and Eucalyptus robusta. The results show that elevated CO2 increased the steady-state photosynthesis rate (A) and decreased stomatal conductance (gs) and the maximum carboxylation rate (Vcmax) in both species. While E. robusta exhibited a decrease in the linear electron transport rate (J) and the fraction of open reaction centers in photosynthesis II (qL), P. tomentosa showed a significant increase in non-photochemical quenching (NPQ). With respect to non-steady-state photosynthesis, elevated CO2 significantly reduced the induction time of A following a shift from low to high light intensity in both species. Time-integrated limitation analysis during induction revealed that elevated CO2 reduces the relative impacts of stomatal limitations in both species, consequently shifting the predominant limitation on induction efficiency from stomatal to biochemical components. Additionally, species-specific changes in qL and NPQ suggest that elevated CO2 may increase biochemical limitation by affecting energy allocation between carbon fixation and photoprotection. These findings suggest that, in a future CO2-rich atmosphere, plants productivity under fluctuating light may be primarily constrained by photochemical and non-photochemical quenching. Full article
(This article belongs to the Special Issue Biological Signaling in Plant Development)
Show Figures

Figure 1

21 pages, 7102 KiB  
Article
Spatial Distribution of Fine Roots in Pinus tabuliformis and Populus tomentosa and Their Competition in Soils Response to Nutrient Availability and Proximity
by Xi Wei, Shuping Wei, Yuan Dong, Lei Jia, Danning Hao and Wenjun Liang
Forests 2024, 15(11), 1895; https://doi.org/10.3390/f15111895 - 28 Oct 2024
Cited by 2 | Viewed by 1169
Abstract
Developing high-efficiency mixed forests or converting low-efficiency pure forests into near-natural mixed forests with optimal structure and function is a crucial aspect of forest management. In the initial stages of afforestation or stand improvement, fertilization and planting distance significantly influence the formation and [...] Read more.
Developing high-efficiency mixed forests or converting low-efficiency pure forests into near-natural mixed forests with optimal structure and function is a crucial aspect of forest management. In the initial stages of afforestation or stand improvement, fertilization and planting distance significantly influence the formation and development of mixed forests. This study investigated how nutrients and planting distance affect root competition between five-year-old Chinese pine (Pinus tabuliformis) and one-year-old Chinese white poplar (Populus tomentosa) and identified the factors influencing the competitive ability of these two species. Field planting experiments used three fertilization gradients (63 g·m−2, 125 g·m−2, and 250 g·m−2) of Stanley compound fertilizer with an N:P:K ratio of 2:1:1 and two planting distances (25 cm and 50 cm). Each experimental group was planted in circular plots with a radius of 0.5 m, yielding a total of nine plots. The effects of different fertilization concentrations and planting distances on root distribution were analyzed both horizontally and vertically. Pearson correlation analysis was used to assess the relationship between roots and soil nutrients, while Levins’ niche overlap formula evaluated the differences in root competition between the species. Furthermore, principal component analysis quantified the relationships between impact factors and the root competitiveness of the two species. Results indicated that both species primarily allocated their fine root biomass to the shallow 0–10 cm layer. Pinus tabuliformis primarily extended to the southwest, while Populus tomentosa predominantly grew to the north. Both species exhibited enhanced root growth at moderate nutrient concentrations of 125 g·m−2. At a planting distance of 50 cm from Pinus tabuliformis, Populus tomentosa seedlings demonstrated superior root growth compared to those planted 25 cm apart. Pinus tabuliformis demonstrated greater competitive ability in the deeper 10–30 cm soil layers compared to Populus tomentosa, which showed the opposite pattern in the shallow 0–10 cm layers. Furthermore, available phosphorus (contribution rate of one impact factor on the competitiveness indexes, CR: −0.998), organic matter (CR: −0.978), total nitrogen (CR: −0.947), and alkali-hydrolysable nitrogen (CR: −0.937) significantly negatively impacted the competitiveness indexes of Pinus tabuliformis. The fine root surface area, volume, and length of Populus tomentosa also significantly negatively affected its competitiveness indexes, with all contribution rates exceeding an absolute value of 0.847. Results indicated that the root distributions of Pinus tabuliformis and Populus tomentosa overlapped spatially, with each species exhibiting advantages in different regions. Therefore, in future plantation reconstruction and forest management, it is essential to thoroughly evaluate root, soil, and fertilizer factors, adjusting planting distances accordingly, to effectively mitigate competition between the two species and successfully establish a mixed forest. Full article
(This article belongs to the Section Forest Soil)
Show Figures

Figure 1

Back to TopTop