Search Results (434)

The subject of this research is the exploration of the potential of remote sensing and Geographic Information Systems (GIS) for basin-scale spatio-temporal monitoring of drought and its impacts in the Ukrina River Basin, Bosnia and Herzegovina (BH), during the last decade (2015–2024). The aim is to integrate meteorological, hydrological, agricultural, and socio-economic drought signals and to delineate areas of long-term drought exposure. Meteorological drought was evaluated using CHIRPS precipitation and the Standardized Precipitation Index (SPI) calculated at 1-, 3-, 6-, and 12- month accumulation scales using Gamma fitting and a fixed long term reference period; hydrological drought was examined using available water-level records complemented by the Standardized Water Level Index (SWLI) and supported by correspondence with standardized ERA5-Land runoff anomalies; agricultural drought was mapped using remote sensing indices—the Temperature Condition Index (TCI), Vegetation Condition Index (VCI), and Vegetation Health Index (VHI)—calculated from MODIS satellite data; and socio-economic effects were assessed using municipal crop-production statistics (2015–2019). The results indicate that drought conditions were most pronounced in 2015, 2017, 2021, and especially 2022, showing consistent agreement between precipitation deficits, hydrological responses, and vegetation stress, while 2016, 2018–2020, 2023, and 2024 were generally more favorable. As a key novelty, a persistent drought-prone zone was delineated by intersecting drought-affected areas across major episodes, providing a basin-scale identification of chronic drought hotspots for a river basin in BH. The persistent zone covers 40.02% of the basin and spans nine cities and municipalities, with >93% located in Prnjavor, Derventa, Stanari, and Teslić. Hotspots are concentrated mainly in lowlands below 400 m a.s.l., with a statistically significant concentration across lower elevation classes, indicating higher long-term exposure in the central and northern valley sectors, and land use overlay further highlights high relative exposure of productive land. Overall, the integrated remote sensing and GIS framework strengthens drought monitoring by providing spatially explicit and repeatable evidence to support targeted adaptation planning and drought-risk management. Full article

Small- and medium-sized enterprises (SMEs) are central to sustainable development in transition economies, yet their financial fragility often limits resilience and the capacity to invest in innovation and responsible practices. Despite growing interest in SME, financial health and its role in sustainability, empirical evidence from small transition economies like Montenegro remains scarce, particularly on how liquidity and profitability dynamics underpin conditions for SDG-aligned growth. This study addresses this gap by analyzing how core financial indicators—cash position, capital structure, and working capital efficiency—affect liquidity and profitability among 345 Montenegrin SMEs across manufacturing, services, and trade. Using OLS and robust regression models, results reveal that a higher cash-to-revenue ratio and moderate leverage significantly enhance both short-term solvency and profitability, while working capital efficiency shows nuanced effects and sector-specific patterns emerge in capital-intensive industries. These findings highlight financial management as a foundation for SME resilience, creating financial preconditions for innovation and digital investments in aligned with SDGs (goals 8, 9, 12). Policy recommendations focus on improving finance access and financial literacy to foster innovation-driven, sustainable SME models aligned with the 2030 Agenda. Full article

Prangos ferulacea (L.) Lindl. (Apiaceae) is an orophilous species with notable traditional uses, particularly across the broader Middle East region. Over the past 50 years, research on its essential oil has revealed the existence of several chemotypes. In addition to its chemical composition, there is also data on the biological activities of the essential oil. Among these activities, the most extensively studied are its antimicrobial and, to a lesser extent, antioxidant properties. Recent findings suggest the presence of additional biological effects, including cytotoxic, insecticidal, and phytotoxic effects. This review summarizes current knowledge and provides a foundation for future research, including more in-depth chemical and chemotaxonomic analyses, as well as exploration of the full therapeutic potential of this species. Full article

Pomegranate is valued for its abundant polyphenolic content and its capacity to promote health. In this study, pomegranate juice or pericarp extracts from two Mediterranean regions (Montenegro and Italy) were systematically and comparatively evaluated for the first time with respect to their polyphenolic composition, antioxidant capacity, and antimicrobial activity. The extraction of juice extracts was accomplished by means of the Kutscher–Steudel liquid–liquid extraction technique, which was employed to selectively recover phenolics. In contrast, the extraction of pericarp extracts from the solid matrix was achieved via Soxhlet extraction. A thorough high-performance liquid chromatography (HPLC) analysis was conducted to identify and quantify the major phenolic compounds present in the sample. This analysis revealed the presence of ellagitannin punicalagin isomers, with concentrations reaching up to 254.75 mg/g of the sample, as well as ellagic acid and gallic acid. The antioxidant potential of the samples was assessed using the antioxidant activity index (AAI) from the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and by a ferric reducing antioxidant power (FRAP) assay. Juice extracts demonstrated a range of activity levels, with AAI values ranging from 0.17 to 2.12 and FRAP values ranging from 2.49 to 19.41 mmol Fe²⁺/g. In contrast, pericarp extracts exhibited notably higher activity, with AAI values ranging from 0.18 to 27.57 and FRAP values ranging from 2.99 to 372.17 mmol Fe²⁺/g. This study demonstrates the markedly higher functional potential of pericarp extracts compared to juice extracts by linking detailed phenolic profiles with bioactivity data. Antimicrobial testing, inclusive of the determination of minimum bactericidal concentration (MBC), demonstrated that certain pericarp extracts manifested bactericidal properties at low concentrations against selected clinically pertinent strains, including methicillin-resistant Staphylococcus aureus (0.109% p/v), methicillin-sensitive S. aureus (0.109% p/v), carbapenem-resistant Acinetobacter baumannii (0.109% p/v), and Escherichia coli (0.563% p/v). Candida albicans and Klebsiella pneumoniae strains exhibited minimal sensitivity to these extracts. The findings indicate that pomegranate pericarp is a valuable by-product, and they demonstrate the potential of both juice and pericarp extracts as functional ingredients. Full article

Background/Objectives: Vedolizumab (VDZ), a monoclonal antibody targeting α4β7 integrin, is used in Crohn’s disease (CD) management, yet patients’ responses vary, underscoring the need for pharmacogenomic (PGx) markers. This study aimed to identify PGx pathways associated with suboptimal VDZ response using a rare-variant analytical framework. Methods: DNA from 63 CD patients treated with VDZ as first-line advanced therapy underwent whole-exome sequencing. Clinical response at week 14 classified patients as optimal responders (ORs) or suboptimal responders (SRs). Sequencing data were processed using GATK Best Practices, annotated with variant effect predictors, and filtered for rare damaging variants (damaging missense and high-confidence loss-of-function; minor allele frequency < 0.05). Variants were mapped to genes specific for SRs and ORs, and analyzed for pathway enrichment using the Reactome database. Rare-variant burden and composition differences were assessed with Fisher’s exact test and SKAT-O gene-set association analysis. Results: Suboptimal VDZ response was associated with pathways related to membrane transport (ABC-family proteins, ion channels), L1–ankyrin interactions, and bile acid recycling, while optimal response was associated with pathways involving MET signaling. SKAT-O identified lipid metabolism-related pathways as significantly different—SRs harbored variants in pro-inflammatory lipid signaling and immune cell trafficking genes (e.g., PIK3CG, CYP4F2, PLA2R1), whereas ORs carried variants in fatty acid oxidation and detoxification genes (e.g., ACADM, CYP1A1, ALDH3A2, DECR1, MMUT). Conclusions: This study underscores the potential of exome-based rare-variant analysis to stratify CD patients and guide precision medicine approaches. The identified genes and pathways are potential PGx markers for CD patients treated with VDZ. Full article

This study aimed to investigate the chemical composition and biological potential of needle extracts from five pine species, including antimicrobial, antioxidant, and cytotoxic activity, as well as their influence on cell cycle progression. Needle extracts were prepared using three extraction methods: conventional maceration (CM), ultrasound-assisted extraction (UAE), and digestion (D). The chemical profile was determined with an emphasis on phenolic acids, flavonoids, and related phenolic compounds. The highest total phenolic content was observed in Pinus sylvestris (3.438 mg/g GAE), followed by Pinus heldreichii (2.732 mg/g GAE). Rutin, ferulic acid, and quercitrin were identified as the predominant phenolic compounds. The highest total flavonoid content was found in Pinus pinea extracts obtained by digestion (1.213 mg/g QE), followed by P. heldreichii (1.074 mg/g QE) and Pinus halepensis (1.074 mg/g QE), both obtained by UAE. Among all examined species, Pinus pinea exhibited the highest TTC values, regardless of the extraction method (7.31–8.21 mg/g GAE). Antibacterial testing showed that P. pinea had an MIC of 19 mg/mL against Enterococcus faecium, while P. sylvestris had the same MIC against Bacillus spizizenii. All extracts exhibited cytotoxic effects using MTT assay against HeLa cells at concentrations of 8%, 16%, and 32%, while LS 174T cells were the least sensitive. Pine needle extracts from Montenegro are a valuable source of phenolic and flavonoid compounds, and they demonstrate antimicrobial and cytotoxic activities. The results support the need for further in vivo studies and elucidation of mechanisms of action in order to assess their potential application as novel bioactive agents. Full article

This pilot study investigated the presence of methicillin-resistant Staphylococcus aureus (MRSA) and third-generation cephalosporin-resistant (3GC-R) Enterobacterales in conventionally kept domestic pigs and their environment across four districts in Rwanda. A total of 114 swabs (nasal, rectal, manure, dust) from 29 farms were collected and processed to isolate resistant bacteria. Thirty-two 3GC-R Enterobacterales were detected. Escherichia coli predominantly harboring bla_CTX-M group 1 β-lactamase genes, alongside Klebsiella pneumoniae isolates, all displaying extended-spectrum β-lactamase (ESBL) phenotypes. Four MRSA isolates, all belonging to clonal complex 398 and SCCmec type IV, the typical livestock MRSA, were recovered from nasal and environmental samples. Multidrug resistance was frequently observed. The co-occurrence of β-lactamase genes, non-β-lactam resistance genes, and virulence factors such as fimH and loci associated with extraintestinal pathogenic and enteropathogenic E. coli. The detection of both MRSA and 3GC-R Enterobacterales in the present study indicates pigs and their farm environments as reservoirs of WHO priority pathogens in Rwanda, highlighting a potential public health risk in the context of extensive human–animal–environment interaction. These findings emphasize the urgent need for integrated One Health surveillance and comprehensive AMR control strategies addressing both animal and environmental reservoirs to support Rwanda’s National Action Plan on Antimicrobial Resistance. Full article

We aimed to investigate the association between oxidative stress (OS), inflammation, and kidney function and the predictive ability of OS for mortality and cardiovascular disease in 143 patients with type 2 diabetes (T2DM) and various degrees of kidney function. At baseline, we assessed catalase, nitrogen oxides (NOx), malondialdehyde (MDA), advanced oxidation products (AOPPs), myeloperoxidase (MPO)], kidney function, and C-reactive protein (CRP). All patients were followed for 57 months, with the combined primary outcome of death/cardiovascular (CV) event, whichever occurred first. NOx was an independent predictor of estimated glomerular filtration rate (B = −0.097, p = 0.006), and MPO was correlated with glycated hemoglobin (r = 0.17, p = 0.046), CRP (r = −0.18, p = 0.032), and serum albumin (r = 0.2, p = 0.011, Spearman’s rho). During the follow-up, 24 composite events were documented. Kaplan–Meier curves showed that smoking (p = 0.029), serum albumin (p = 0.014), and MPO (p = 0.024, log-rank test) were associated with the outcome. In multivariate Cox regression models, smoking and MPO were independent predictors of the composite outcome (hazard ratio—HR = 2.8, p = 0.004, 955 confidence interval—CI 1.05–7.5 and HR = 0.99, p = 0.015, 95% CI: 0.98–1.00, respectively), after adjustment for several cofactors. OS might be associated with CV disease in T2DM. Full article

Background: Falls are a leading cause of injury, disability, and loss of independence among community-dwelling older adults. Although exercise-based interventions are widely recommended for fall prevention, the comparative effectiveness of different exercise modalities remains insufficiently synthesized. Methods: This systematic review was conducted in accordance with PRISMA 2020 guidelines. Randomized and controlled trials were identified through searches of PubMed, Scopus, Web of Science, and CINAHL, including studies involving adults aged ≥60 years who participated in balance-based, strength-based, multimodal, Tai Ji Quan, Otago Exercise Program, or perturbation-based reactive balance training interventions. Methodological quality was assessed using the Cochrane Risk of Bias 2 (ROB-2) tool. Due to substantial clinical and methodological heterogeneity across interventions and outcome measures, a narrative synthesis was undertaken. Results: Twenty-seven trials met the inclusion criteria. Exercise interventions consistently reduced fall incidence across studies. Tai Ji Quan interventions were associated with approximately 31–58% reductions in falls, the Otago Exercise Program with 23–40% reductions, and multimodal strength–balance training with 20–45% reductions. Perturbation-based reactive balance training demonstrated particularly strong effects on laboratory-induced falls, with reductions ranging from 50–75%. Functional outcomes also improved across intervention types, including faster Timed Up and Go performance, increased gait speed, improvements of approximately 1.2–2.5 points in Short Physical Performance Battery scores, 15–35% gains in lower-limb strength, and enhanced reactive balance responses. Longer-duration interventions (12–24 months) generally demonstrated sustained reductions in fall risk. Conclusions: Evidence from randomized and controlled trials indicates that structured exercise interventions, particularly Tai Ji Quan, the Otago Exercise Program, multimodal strength–balance training, and perturbation-based reactive balance training, are effective in reducing falls and improving balance, mobility, and strength in community-dwelling older adults. These findings support the use of targeted, evidence-based exercise programs as central components of fall-prevention strategies in older populations. Full article

Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) commonly coexists with type 2 diabetes (T2D), but their independent contributions to redox imbalance, inflammation and immune signaling remain uncertain. Objectives: This study aimed to evaluate whether the presence of MASLD alone, and the presence of T2D within MASLD, are independently associated with high-risk profiles of oxidative/antioxidant markers, peripheral blood mononuclear cell (PBMC) gene expression and adipocytokines. Methods: A total of 190 participants were categorized via abdominal ultrasound as controls (n = 46), MASLD (n = 83) or MASLD with T2D (n = 61). Measurements included advanced oxidation protein products (AOPP) and paraoxonase-1 (PON1) activity in serum; messenger ribonucleic acids expression of cluster of differentiation 36 (CD36), Toll-like receptor 9 (TLR9), and glutathione peroxidase-1 in PBMC; and adiponectin, leptin, and resistin in plasma. Biomarker values were adjusted and statistical comparisons among groups were performed using the Quade test. Subsequently, biomarkers were stratified into tertiles to examine associations between high-risk biomarker levels and the presence of MASLD or T2D in patients with MASLD using multivariate binary logistic regression. Results: Multivariate analysis showed that MASLD presence was independently associated with both increased AOPP and decreased resistin levels in the circulation. Furthermore, T2D presence in patients with MASLD was independently associated with increased CD36 and decreased TLR9 gene expression in PBMCs, as well as elevated circulating leptin levels. Conclusions: Collectively, these findings underscore the complex interplay between oxidative stress, insulin resistance, inflammation, and immune signaling in the pathogenesis of MASLD, which are fundamental factors contributing to this condition. Full article

This study examines the determinants of tolerance among higher education students in Montenegro and their implications for educational and societal sustainability. Guided by the framework of Education for Sustainable Development (ESD), it investigates how socio-demographic factors, economic insecurity, political orientations, and digital media use shape attitudes toward ethnic, religious, and cultural diversity. Cross-sectional survey data were collected from 469 students in 2021 and analysed using binary logistic regression. Results show that education is the strongest predictor of tolerant attitudes (p < 0.01), highlighting the transformative role of higher education in fostering inclusive and sustainability-relevant competencies. Economic insecurity, particularly unemployment, was associated with more exclusionary views, linking social equity to sustainability outcomes. Gender (p < 0.001), age (p = 0.07), and engagement with human-rights content online (p < 0.01) also emerged as significant predictors. Religiosity showed a modest negative association with tolerance (p = 0.01). The final model explained 37% of the variance in tolerant attitudes (Nagelkerke R² = 0.37). Digital media played an ambivalent role: while it increased exposure to diverse perspectives, it also contributed to polarization, underscoring the need for critical digital literacy within ESD-aligned curricula. Overall, the findings demonstrate that inclusive education, digital competence, and participatory learning environments are central to building tolerant, cohesive, and sustainability-oriented societies. The study contributes to ESD scholarship by linking social inclusion, sustainability competencies, and the role of higher education in post-transition contexts. Full article

Background: Periprosthetic joint infection (PJI) remains one of the most severe complications after total joint arthroplasty, causing significant morbidity and healthcare burden. Despite advances in surgical techniques, diagnostics, and antimicrobial therapy, infection rates have not declined substantially, emphasizing the need for comprehensive preventive and therapeutic strategies. Methods: This narrative review synthesizes evidence from peer-reviewed clinical studies, consensus statements, and major international guidelines addressing periprosthetic joint infection in hip, knee, and shoulder arthroplasty. Results: Recent evidence highlights advances in diagnostic biomarkers, molecular testing, and standardized consensus criteria that have improved early detection. Surgical strategies such as DAIR, one-stage, and two-stage revisions—combined with biofilm-active antimicrobial therapy—remain the mainstay of management. Preventive measures focusing on perioperative optimization, infection control, and antibiotic stewardship are the most effective means to reduce infection risk. Conclusions: Future progress relies on precision-based prevention, novel biofilm-targeted therapies, and multidisciplinary collaboration to achieve durable, infection-free outcomes following joint arthroplasty. Full article

Bosch–Boonstra–Schaaf optic atrophy syndrome (BBSOAS) is an extremely rare neurological condition caused by a disruption in the NR2F-1 gene. The most common clinical features are optic atrophy and intellectual and developmental delay. This case report aims to describe the cognitive and language profile of a six-year-old girl diagnosed with BBSOAS, with a focus on the syndrome’s impact on her developmental outcomes. A detailed assessment of her cognitive and speech–language abilities is provided. Given the limited number of published case studies on BBSOAS, this report integrates relevant findings from the literature, including information on epidemiology, diagnostics, clinical manifestations, and developmental outcomes. It contributes to the expansion of the known mutational spectrum of BBSOAS, in addition to documenting its phenotypic presentation of cognitive and speech–language development. The case is analyzed within the context of current evidence, emphasizing the importance of early assessment, individualized intervention, ongoing developmental monitoring, and the potential for tailored support to promote optimal developmental outcomes. Full article

Cardiovascular (CV) disease, chronic kidney disease, obesity, and diabetes mellitus have reached epidemic proportions over the past few decades. Accumulating evidence highlights the strong interconnection between these conditions, leading to the definition of a broader disease entity known as cardio-renal-metabolic (CRM) syndrome. This newly recognized clinical entity presents important challenges in identifying the optimal treatment strategy within a holistic, patient-centered framework. In line with this, sodium glucose cotransporter 2 inhibitors (SGLT2is), owing to their multifaceted pharmacological effects, have been suggested as possible treatment options in the management of CRM. SGLT2is exert their antihyperglycemic effects by impeding the renal reabsorption of sodium and glucose, causing glycosuria and natriuresis. Research has confirmed that their unique beneficial effects extend beyond glycemic control, reducing CV death and hospitalizations in patients with heart failure, and the incidence of kidney failure in dedicated kidney outcome studies—regardless of diabetes status. Furthermore, these agents contribute to weight loss and blood pressure reduction. Their benefits appear to stem from a combination of factors, which include reduced oxidative stress, lower levels of inflammation, regulated neurohormonal activation, improved endothelial function, and enhanced metabolic efficiency. This review aims to provide a comprehensive analysis of the pathophysiological mechanisms underlying the effects of SGLT2is in CRM syndrome, synthesize evidence from landmark clinical trials, evaluate current experimental and diagnostic approaches, and provide the emerging role of SGLT2is in the treatment of this new clinical entity. Full article

Background: Emotion regulation (ER) difficulties are closely linked to maladaptive coping strategies, including impulsive and emotional eating, which undermine health and well-being in young adults. Technical university students are particularly vulnerable due to factors such as a high academic workload, sedentary behavior, and performance-related stress. This study evaluated the effects of a four-week structured physical activity intervention on ER and eating behaviors among engineering students. Methods: Seventy first- and second-year computer science and engineering students (40 males and 30 females, aged 19–25 years) from Politehnica University of Bucharest participated in the study. The intervention included three weekly supervised training sessions and a daily step count requirement (≥6000 steps), verified via weekly smartphone submissions. Pre- and post-intervention assessments employed the Difficulties in Emotion Regulation Scale (DERS-36) and the Adult Eating Behavior Questionnaire (AEBQ-35). Data were analyzed using Kolmogorov–Smirnov tests, Wilcoxon signed-rank tests, and paired-sample t-tests. Results: Significant improvements were observed in five ER domains—non-acceptance of emotional responses, goal-directed behavior, impulse control, access to regulation strategies, and emotional clarity (all p < 0.01). No change occurred in emotional awareness (p > 0.05). Eating behaviors (restrained, emotional, and external eating) showed no significant differences pre- and post-intervention (all p > 0.05). Conclusions: A short-term, structured physical activity program enhanced emotion regulation capacities but did not alter eating behaviors in the short run. These findings highlight the feasibility of embedding low-cost, exercise-based modules into higher education to strengthen students’ psychological resilience. Longer and multimodal interventions may be required to produce measurable changes in eating behaviors. Full article