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Keywords = Platform Screen Door

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16 pages, 2839 KB  
Article
Particulate Matter Migration in Subway Tunnels: Experimental and Numerical Investigation
by Haiying Wang, Yifeng Wang, Chudong Hu, Yan Wu and Jianbin Zang
Atmosphere 2026, 17(3), 283; https://doi.org/10.3390/atmos17030283 - 10 Mar 2026
Viewed by 604
Abstract
Platform screen door (PSD) systems can reduce particulate matter (PM) levels at subway platforms, but transient particle migration between tunnels and platforms still occurs during door operation. Existing control measures, such as tunnel cleaning, ventilation optimization, onboard dust removal devices, and air curtain [...] Read more.
Platform screen door (PSD) systems can reduce particulate matter (PM) levels at subway platforms, but transient particle migration between tunnels and platforms still occurs during door operation. Existing control measures, such as tunnel cleaning, ventilation optimization, onboard dust removal devices, and air curtain systems, mainly target background PM concentrations and generally function as passive mitigation strategies. However, the transient dynamics of tunnel-to-platform PM migration during PSD operation remain insufficiently understood. In this study, field measurements and numerical simulations were used to investigate PM migration under realistic subway operating conditions. Field observations were conducted to characterize the spatial distribution of PM and its relationship with tunnel piston wind. A numerical model based on the Discrete Phase Model (DPM) was then developed to simulate particle transport under different PSD operating sequences. The effects of PSD opening delay and opening duration on particle migration were examined to evaluate their influence on migration rates. The results show that adjusting the timing of PSD operation can significantly reduce tunnel-to-platform PM migration, whereas conventional air curtain configurations may enhance interzonal particle exchange under certain conditions. These findings improve the understanding of PSD-related PM transport and provide potential operational strategies for improving air quality in underground rail transit systems. Full article
(This article belongs to the Section Air Quality)
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16 pages, 1213 KB  
Article
Impact of Subway Platform Screen Door Opening and Closing on Particulate Matter Concentration Distribution at Different Locations and Times: The Case of Xi’an
by Liang Xian, Yonghao Yuan and Xin Zhang
Buildings 2026, 16(2), 356; https://doi.org/10.3390/buildings16020356 - 15 Jan 2026
Cited by 2 | Viewed by 1188
Abstract
To explore how the opening and closing of subway platform screen doors (PSD) affect particulate matter concentrations (PM10, PM2.5, and PM1.0) across different times (morning peak, off-peak, evening peak), and three key locations (subway tunnels, platforms, and [...] Read more.
To explore how the opening and closing of subway platform screen doors (PSD) affect particulate matter concentrations (PM10, PM2.5, and PM1.0) across different times (morning peak, off-peak, evening peak), and three key locations (subway tunnels, platforms, and waiting areas), we studied the Xi’an subway using a systematic monitoring approach, and a total of 6 monitoring points were monitored at 3 locations for 60 consecutive days of testing. The sampling time for each measurement point was 20 min, and a total of three groups were tested. The relationship between the opening/closing status of PSD and changes in particulate matter concentrations was then analyzed using statistical methods. The results showed that the particulate matter concentrations followed a sequential pattern: tunnel concentrations were higher than those in the waiting area, which in turn were higher than those at the platform center. PM10 concentrations exceeded China’s standards for indoor air quality (GB/T 18883-2022) at all three locations. For PM2.5, concentrations in the tunnel and waiting area exceeded the standard, while those at the platform center remained within the limit. Particles smaller than 1.0 μm constituted the dominant fraction of particulate matter in the tunnel, waiting area, and platform center. After the PSD opened, the peak average concentrations of PM10, PM2.5, and PM1.0 in the waiting area increased by 70.53%, 55.81%, and 42.41%, respectively, compared to the average concentrations before the train entered the station. PSD had a significant impact on fine particulate matter concentrations on the platform during the evening peak: PM10 concentrations in the front and rear of the waiting area were 37.85% and 57.61% higher than those in the middle, while PM2.5 concentrations in these two areas were 39.81% and 50.23% higher than in the middle. No obvious distribution pattern was observed for PM1.0. These results provide reference data for optimizing indoor air quality in the Xi’an subway and regulating the operation of platform screen doors. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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13 pages, 1346 KB  
Proceeding Paper
Assessment of Passenger Obstruction-Related Risk Factors in an Urban Metro Rail Transit System and Their Countermeasures
by Nida Saleh, Qamar Mahboob, Sanan Tahir, Zhiwen Wang, Zidong Tan, Daijun Cheng and Xuefeng Luo
Eng. Proc. 2025, 111(1), 13; https://doi.org/10.3390/engproc2025111013 - 17 Oct 2025
Viewed by 1238
Abstract
Modern metro rail systems have problems concerning the safety of passengers and the operational efficiency. Among these, passenger obstruction is a major challenge which refers to the unintentional or intentional interference of passengers in train and platform screen doors, while boarding or alighting [...] Read more.
Modern metro rail systems have problems concerning the safety of passengers and the operational efficiency. Among these, passenger obstruction is a major challenge which refers to the unintentional or intentional interference of passengers in train and platform screen doors, while boarding or alighting from the trains. This paper provides a risk assessment of passenger obstruction at Orange Line Metro Rail Transit System (OLMRTS) in Lahore, Pakistan. This study adopted structured observations, incident analysis and review by experts to control the obstruction cases. Both quantitative and qualitative data analyses of obstruction cases were performed to evaluate the key risk factors associated with passenger obstructions in OLMRTS. Based on the risk assessment framework, prioritized countermeasures with higher risk reduction impacts have been proposed. The effectiveness of the countermeasures was evident in the substantial reduction in obstruction cases by 80%. This research paper will present a reduction in safety risks by reducing the likelihood of incidents and without compromising the passenger service of OLMRTS. Full article
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21 pages, 3489 KB  
Article
GA-YOLOv11: A Lightweight Subway Foreign Object Detection Model Based on Improved YOLOv11
by Ning Guo, Min Huang and Wensheng Wang
Sensors 2025, 25(19), 6137; https://doi.org/10.3390/s25196137 - 4 Oct 2025
Cited by 4 | Viewed by 2630
Abstract
Modern subway platforms are generally equipped with platform screen door systems to enhance safety, but the gap between the platform screen doors and train doors may cause passengers or objects to become trapped, leading to accidents. Addressing the issues of excessive parameter counts [...] Read more.
Modern subway platforms are generally equipped with platform screen door systems to enhance safety, but the gap between the platform screen doors and train doors may cause passengers or objects to become trapped, leading to accidents. Addressing the issues of excessive parameter counts and computational complexity in existing foreign object intrusion detection algorithms, as well as false positives and false negatives for small objects, this article introduces a lightweight deep learning model based on YOLOv11n, named GA-YOLOv11. First, a lightweight GhostConv convolution module is introduced into the backbone network to reduce computational resource waste in irrelevant areas, thereby lowering model complexity and computational load. Additionally, the GAM attention mechanism is incorporated into the head network to enhance the model’s ability to distinguish features, enabling precise identification of object location and category, and significantly reducing the probability of false positives and false negatives. Experimental results demonstrate that in comparison to the original YOLOv11n model, the improved model achieves 3.3%, 3.2%, 1.2%, and 3.5% improvements in precision, recall, mAP@0.5, and mAP@0.5: 0.95, respectively. In contrast to the original YOLOv11n model, the number of parameters and GFLOPs were reduced by 18% and 7.9%, respectfully, while maintaining the same model size. The improved model is more lightweight while ensuring real-time performance and accuracy, designed for detecting foreign objects in subway platform gaps. Full article
(This article belongs to the Special Issue Image Processing and Analysis for Object Detection: 3rd Edition)
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24 pages, 1905 KB  
Systematic Review
Strategies for Reducing Suicide at Railroads: A Scoping Review of Evidence and Gaps
by Pooja Belur, Patrick Sherry, Ivan Rodriguez, Chetan Kurkure and Shashank V. Joshi
Int. J. Environ. Res. Public Health 2025, 22(1), 18; https://doi.org/10.3390/ijerph22010018 - 27 Dec 2024
Cited by 4 | Viewed by 5310
Abstract
This review aims to systematically evaluate existing literature on reducing suicides along railroads, with specific focus on effectiveness, limitations, and research gaps in the current evidence base. Database searches were conducted in PubMed, PsycInfo, Scopus, Embase, and CINAHL covering studies published until 30 [...] Read more.
This review aims to systematically evaluate existing literature on reducing suicides along railroads, with specific focus on effectiveness, limitations, and research gaps in the current evidence base. Database searches were conducted in PubMed, PsycInfo, Scopus, Embase, and CINAHL covering studies published until 30 November 2024. After screening 623 studies and their references, 51 studies were included; 26 empirically assessed rail-related prevention interventions and 25 provided relevant qualitative insights. Physical barriers like removal of grade crossings, addition of fencing, and platform screen doors (PSDs) showed significant promise. Full-height PSDs eliminated all suicides and half-height PSDs significantly reduced suicide incidence. Fencing was found to be effective but raised concerns about feasibility and must be part of a comprehensive approach to mitigate potential displacement. Safe media reporting was linked to decreased suicides and a reduced risk of contagion, and CCTV monitoring and suicide pits also showed potential but had limited research. Other strategies showed mixed evidence and required additional evaluation. Some studies, particularly on physical barriers, showed possible displacement effects to other stations, highlighting the need for studies larger in geographic and temporal scope. Our findings support certain prevention interventions, but generalizability is limited by scope of research and methodological concerns. Overall, our findings highlight the need for broader, long-term studies to confirm efficacy and establish comprehensive, scalable approaches for policy implementation. Full article
(This article belongs to the Section Health Care Sciences)
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31 pages, 17520 KB  
Article
Sparse Temporal Data-Driven SSA-CNN-LSTM-Based Fault Prediction of Electromechanical Equipment in Rail Transit Stations
by Jing Xiong, Youchao Sun, Junzhou Sun, Yongbing Wan and Gang Yu
Appl. Sci. 2024, 14(18), 8156; https://doi.org/10.3390/app14188156 - 11 Sep 2024
Cited by 7 | Viewed by 2480
Abstract
Mechanical and electrical equipment is an important component of urban rail transit stations, and the service capacity of stations is affected by its reliability. To solve the problem of predicting faults in station mechanical and electrical equipment with sparse data, this study proposes [...] Read more.
Mechanical and electrical equipment is an important component of urban rail transit stations, and the service capacity of stations is affected by its reliability. To solve the problem of predicting faults in station mechanical and electrical equipment with sparse data, this study proposes a fault prediction framework based on SSA-CNN-LSTM. Firstly, this article proposes a fault enhancement method for station electromechanical equipment based on TimeGAN, which expands and generates data that conform to the temporal characteristics of the original dataset, to solve the problem of sparse data in the original fault dataset. An SSA-CNN-LSTM model is then established to extract effective data features from low-dimensional data with insufficient feature depth through structures such as convolutional layers and pooling layers in a CNN, determine the optimal hyperparameters, automatically optimize the model network size, solve the problem of the difficult determination of the neural network model size, and achieve accurate prediction of the fault rate of station electromechanical equipment. Finally, an engineering verification was conducted on the platform screen door (PSD) systems in stations on Shanghai Metro Lines 1, 5, 9, and 10. The experiments showed that the proposed prediction method improved the RMSE by 0.000699, the MAE by 0.00042, and the R2 index by 0.109779 when predicting the fault rate data of platform screen doors on all of the lines. When predicting the fault rate data of the screen doors on a single line, the performance of the model was better than that of the CNN-LSTM model optimized with the PSO algorithm. Full article
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6 pages, 1790 KB  
Proceeding Paper
Station Passenger Barrier Systems and Their Impact on Metro Transport Services
by Svetoslav Tomov and Emiliya Dimitrova
Eng. Proc. 2024, 70(1), 56; https://doi.org/10.3390/engproc2024070056 - 28 Aug 2024
Cited by 5 | Viewed by 4995
Abstract
The prevention of passengers’ access to the tracks is crucial for current urban railway transport. The “Safety first” principal led to the need to separate the platform from the train tracks as a measure of passengers’ protection. Due to this, many train stations [...] Read more.
The prevention of passengers’ access to the tracks is crucial for current urban railway transport. The “Safety first” principal led to the need to separate the platform from the train tracks as a measure of passengers’ protection. Due to this, many train stations around the world are equipped with barrier (screen) security systems. However, the requirements for passengers’ comfort are high as well. Automated transport should ensure the trains are on time and passengers’ exchange at stations is smooth. Therefore, it is necessary that station passenger barrier systems comply with the line signalling systems. Preventing or reducing additional delays is essential to provide efficient transport services and maximum line capacity while ensuring passenger safety. This report provides the operation outcomes for different train lines with implemented advanced station barrier systems for passengers—automatic platform doors (vertical or horizontal) and beam barriers—and indicates the strong and weak points of the given solutions. Full article
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19 pages, 1462 KB  
Article
STPA-RL: Integrating Reinforcement Learning into STPA for Loss Scenario Exploration
by Jiyoung Chang, Ryeonggu Kwon and Gihwon Kwon
Appl. Sci. 2024, 14(7), 2916; https://doi.org/10.3390/app14072916 - 29 Mar 2024
Cited by 3 | Viewed by 2609
Abstract
Experience-based methods like reinforcement learning (RL) are often deemed less suitable for the safety field due to concerns about potential safety issues. To bridge this gap, we introduce STPA-RL, a methodology that integrates RL with System-Theoretic Process Analysis (STPA). STPA is a safety [...] Read more.
Experience-based methods like reinforcement learning (RL) are often deemed less suitable for the safety field due to concerns about potential safety issues. To bridge this gap, we introduce STPA-RL, a methodology that integrates RL with System-Theoretic Process Analysis (STPA). STPA is a safety analysis technique that identifies causative factors leading to unsafe control actions and system hazards through loss scenarios. In the context of STPA-RL, we formalize the Markov Decision Process based on STPA analysis results to incorporate control algorithms into the system environment. The agent learns safe actions through reward-based learning, tracking potential hazard paths to validate system safety. Specifically, by analyzing various loss scenarios related to the Platform Screen Door, we assess the applicability of the proposed approach by evaluating hazard trajectory graphs and hazard frequencies in the system. This paper streamlines the RL process for loss scenario identification through STPA, contributing to self-guided loss scenarios and diverse system modeling. Additionally, it offers effective simulations for proactive development to enhance system safety and provide practical assistance in the safety field. Full article
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19 pages, 2286 KB  
Review
Demand-Oriented Review of a Dynamic Energy-Loss Monitoring System for Primary School Buildings through Micro-Environmental Data Monitoring and Occupant Behavior Analysis
by Zhen Peng, Yanan Yu and Rui Guan
Buildings 2023, 13(11), 2694; https://doi.org/10.3390/buildings13112694 - 25 Oct 2023
Cited by 3 | Viewed by 1829
Abstract
The utilization of primary school buildings is multifaceted, primarily due to the high occupancy density, varying thermal preferences among occupants, diverse indoor activities (such as walking, sports, and conversation), and a constant flow of individuals entering and exiting the building. This results in [...] Read more.
The utilization of primary school buildings is multifaceted, primarily due to the high occupancy density, varying thermal preferences among occupants, diverse indoor activities (such as walking, sports, and conversation), and a constant flow of individuals entering and exiting the building. This results in the frequent opening and closing of external windows and doors and fluctuations in internal heat gain. Consequently, frequent interactions between the indoor and outdoor microenvironments lead to energy losses. This study conducts a comprehensive literature review on building energy loss stemming from occupant behavior and the interactions between indoor and outdoor microenvironments. Furthermore, it proposes a dynamic real-time monitoring system based on a foundation of computer data capture and a visualization platform for building energy loss. The research methods include data crawling, data association rule mining, and data association analysis. The research findings yield a universally applicable and informative building energy-saving design system based on extensive data analysis. Additionally, the system presents information on occupants’ behavior and the microclimate data of indoor and outdoor environments on a computer screen, facilitating human–machine communication and enabling timely adjustments to be made, thus facilitating the construction of design strategies for new buildings and operation and maintenance strategies for existing buildings. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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13 pages, 4066 KB  
Article
Simulation of Piston Effects on Platform Screen Doors Considering Air Leakage
by Jian Zhang, Jianyao Wang, Qingshan Yang and Qiusheng Li
Atmosphere 2022, 13(12), 1967; https://doi.org/10.3390/atmos13121967 - 25 Nov 2022
Cited by 1 | Viewed by 3882
Abstract
The complex wind effects around platform screen doors (PSDs) caused by train-induced piston wind effect and positive micropressure waves in subway station platforms are investigated. Numerical modeling of the wind field around full-scale PSDs with real gaps under different inflow conditions is developed [...] Read more.
The complex wind effects around platform screen doors (PSDs) caused by train-induced piston wind effect and positive micropressure waves in subway station platforms are investigated. Numerical modeling of the wind field around full-scale PSDs with real gaps under different inflow conditions is developed to analyze the pressure distributions on and around the PSDs and the corresponding recirculation regions in the frontal and rear PSD areas with computational fluid dynamics (CFD) method. An equivalent porous media model is developed to obtain the relationship between the pressure difference and wind velocity based on Darcy–Forchheimer’s Law. It includes a viscosity loss term and an inertial loss term in the simulation of the air leakage flow generated from the PSD gap. The coefficients of these two terms are estimated from the CFD results from the full-scale models. The complicated flow field originated from the gaps is the main cause of the large wind pressure on the PSD, and the flow velocity on the platform may significantly affect the comfort of pedestrians and of the safety design of the PSD system. Full article
(This article belongs to the Special Issue Advances in Computational Wind Engineering and Wind Energy)
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20 pages, 8284 KB  
Article
Reducing PM10 and PM2.5 Concentrations in a Subway Station by Changing the Diffuser Arrangement
by Seong-Gyu Kim, Gibong Sung, Se-Jin Yook, Minjeong Kim and Duckshin Park
Toxics 2022, 10(9), 537; https://doi.org/10.3390/toxics10090537 - 15 Sep 2022
Cited by 8 | Viewed by 4191
Abstract
According to the stringent regulations on particulate matter (PM) concentrations in Seoul, Korea, the PM10 and PM2.5 concentrations in subway stations must be maintained below 50 and 30 μg/m3, respectively, by 2024. Therefore, the PM concentrations in a subway [...] Read more.
According to the stringent regulations on particulate matter (PM) concentrations in Seoul, Korea, the PM10 and PM2.5 concentrations in subway stations must be maintained below 50 and 30 μg/m3, respectively, by 2024. Therefore, the PM concentrations in a subway station were analyzed considering air-conditioning diffuser arrangement and filtration efficiency, with the total ventilation flow rate of the station maintained constant. Dynamic analysis was performed under a worst-case scenario, wherein outdoor air was introduced through ground entrances and high-concentration dust (PM10, PM2.5) was introduced from stationary train cabins into the platforms through open platform screen doors (PSDs). Although the average PM concentrations were predicted to satisfy the reinforced criteria of Seoul under the existing operating conditions, the recommended limits were exceeded in certain local areas. To address this, the PM concentrations were predicted by changing the diffuser arrangement in the waiting room and maintaining the total ventilation flow rate constant. When the diffusers were placed near the waiting room walls, the PM10 and PM2.5 concentrations were reduced by approximately 10.5 and 5%, respectively, compared to the previous diffuser arrangement. Thus, the required PM concentration criteria were satisfied in nearly all areas of the target station, except for certain areas close to PSDs. The study findings can form the basis for improving the air quality of other subway stations. Full article
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29 pages, 13301 KB  
Article
Numerical Study on the Impact of Platform Screen Doors in a Subway Station with a Train on Fire
by Catalin Ioan Teodosiu and Vladimir Francisc Kubinyecz
Appl. Sci. 2022, 12(16), 8296; https://doi.org/10.3390/app12168296 - 19 Aug 2022
Cited by 10 | Viewed by 8049
Abstract
Almost all recently built subway stations are equipped with Platform Screen Doors (PSDs) due to the numerous proven benefits of these systems. In addition, PSDs are now being introduced in existing subway stations, but their operation in conjunction with previously designed ventilation systems [...] Read more.
Almost all recently built subway stations are equipped with Platform Screen Doors (PSDs) due to the numerous proven benefits of these systems. In addition, PSDs are now being introduced in existing subway stations, but their operation in conjunction with previously designed ventilation systems in case of emergency should be deeply studied. In this context, the objective of this study is to assess the efficiency of the planned emergency strategy (coupled operation, ventilation systems–PSDs system) in the case of trains on fire stopped at the platform of a subway station retrofitted with PSDs. The approach is based on Computational Fluid Dynamics (CFD) full-scale simulations to predict the airflow, temperature, and pollutant (carbon monoxide—CO and carbon dioxide—CO2) concentrations caused by the fire. The results show the evident contribution of PSDs in stopping the dispersion of hot and polluted air in the subway station during the entire simulation time (20 min from the arrival of the train on fire). Consequently, the investigated emergency strategy (exhausting air both through the “over track system” and the “under platform system”, simultaneously with the opening of the PSDs on the side with the train on fire) assures the safe evacuation of passengers as soon as they have left the subway train. The results indicate that access to the platform is not perturbed by high temperatures or dangerous concentrations of CO and CO2. Full article
(This article belongs to the Special Issue Urban Sustainability and Resilience of the Built Environments)
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19 pages, 7088 KB  
Article
Comprehensive Evaluation of the Visual Environment of a Railroad Station’s Platform, Focused on the User’s Psychology
by Jaeyoung Heo, Miki Kozaki, Takaaki Koga, Kotaroh Hirate and Hyun Bae Kim
Appl. Sci. 2021, 11(14), 6566; https://doi.org/10.3390/app11146566 - 16 Jul 2021
Cited by 6 | Viewed by 3241
Abstract
Through a comprehensive evaluation of the spaces of 25 railway stations, where power-saving operations are being conducted due to an earthquake, the effects of various components of the space on users’ psychology were reviewed. For the platforms of railway stations, which are divided [...] Read more.
Through a comprehensive evaluation of the spaces of 25 railway stations, where power-saving operations are being conducted due to an earthquake, the effects of various components of the space on users’ psychology were reviewed. For the platforms of railway stations, which are divided into ‘island platforms’ and ‘side platforms’ the ‘physical quantity measurement’ and ‘impression evaluation’ were assessed. When a simple power-saving method, such as partial lighting up is adopted, it has been shown that there is a negative effect on the users, both physically and psychologically. In particular, in the case of outdoor platforms, there is a concern that glare and anxiety due to contrast may increase; therefore, it is necessary to utilize the reflective surfaces effectively. Floor illuminance is fundamental to lighting design. However, some areas cannot be evaluated by floor illuminance alone. The difference in the results of the impression evaluation in five areas with floor illuminance around 50 lx was widely distributed, and it was found that the shape and material of the space affected the results. If platform screen doors are installed, a lighting method that can evenly illuminate the interior of the space is necessary. By reviewing each factor that determines the visual impression, it was revealed that the lighting location and lighting method were significantly affected. Full article
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15 pages, 2680 KB  
Article
Experimental Investigation of Air Quality in a Subway Station with Fully Enclosed Platform Screen Doors
by Liping Pang, Chenyuan Yang, Xiaodong Cao, Qing Tian and Bo Li
Int. J. Environ. Res. Public Health 2020, 17(14), 5213; https://doi.org/10.3390/ijerph17145213 - 19 Jul 2020
Cited by 18 | Viewed by 4344
Abstract
In this study, the indoor air quality (IAQ) was investigated in a subway station with fully enclosed platform screen doors in Beijing, China. Eight indoor air pollutants, including PM2.5, PM10, SO2 (sulfur dioxide), NO2 (nitrogen dioxide), NH [...] Read more.
In this study, the indoor air quality (IAQ) was investigated in a subway station with fully enclosed platform screen doors in Beijing, China. Eight indoor air pollutants, including PM2.5, PM10, SO2 (sulfur dioxide), NO2 (nitrogen dioxide), NH3 (ammonia), CO (carbon monoxide), CH2O (formaldehyde) and TVOC (total volatile organic compound), were measured for six consecutive days in October 2019. The results indicated that the IAQ in the subway station was basically stable at good levels for most times during the whole measurement period. All eight indoor air pollutants were far below their corresponding maximum allowable concentrations, except for the PM2.5 concentrations, which occasionally exceeded the concentration limits. The concentrations of indoor air pollutants in the subway station were basically within the corresponding standards. The correlation analyses showed that outdoor air pollutants have important influences on indoor air pollutants. The concentrations of PM10, PM2.5, SO2, NO2 and CO in the subway station were positively correlated with their corresponding outdoor concentrations. PM10 was statistically significantly correlated with the passenger flow and train frequency, but the other air pollutants were less impacted by the passenger flow and train frequency. Full article
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25 pages, 669 KB  
Review
Key Challenges in Designing CHO Chassis Platforms
by Anis Hamdi, Diana Széliová, David E. Ruckerbauer, Isabel Rocha, Nicole Borth and Jürgen Zanghellini
Processes 2020, 8(6), 643; https://doi.org/10.3390/pr8060643 - 28 May 2020
Cited by 14 | Viewed by 11355
Abstract
Following the success of and the high demand for recombinant protein-based therapeutics during the last 25 years, the pharmaceutical industry has invested significantly in the development of novel treatments based on biologics. Mammalian cells are the major production systems for these complex biopharmaceuticals, [...] Read more.
Following the success of and the high demand for recombinant protein-based therapeutics during the last 25 years, the pharmaceutical industry has invested significantly in the development of novel treatments based on biologics. Mammalian cells are the major production systems for these complex biopharmaceuticals, with Chinese hamster ovary (CHO) cell lines as the most important players. Over the years, various engineering strategies and modeling approaches have been used to improve microbial production platforms, such as bacteria and yeasts, as well as to create pre-optimized chassis host strains. However, the complexity of mammalian cells curtailed the optimization of these host cells by metabolic engineering. Most of the improvements of titer and productivity were achieved by media optimization and large-scale screening of producer clones. The advances made in recent years now open the door to again consider the potential application of systems biology approaches and metabolic engineering also to CHO. The availability of a reference genome sequence, genome-scale metabolic models and the growing number of various “omics” datasets can help overcome the complexity of CHO cells and support design strategies to boost their production performance. Modular design approaches applied to engineer industrially relevant cell lines have evolved to reduce the time and effort needed for the generation of new producer cells and to allow the achievement of desired product titers and quality. Nevertheless, important steps to enable the design of a chassis platform similar to those in use in the microbial world are still missing. In this review, we highlight the importance of mammalian cellular platforms for the production of biopharmaceuticals and compare them to microbial platforms, with an emphasis on describing novel approaches and discussing still open questions that need to be resolved to reach the objective of designing enhanced modular chassis CHO cell lines. Full article
(This article belongs to the Collection Principles of Modular Design and Control in Complex Systems)
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