Search Results (85)

The objective of this study was to evaluate the serum biomarkers implicated in the interaction of platelets and endothelium, as well as the efficacy of antiplatelet therapy in patients with aortic stenosis (AS) and coronary artery disease (CAD). A total of 78 adult patients with CAD on aspirin therapy participated in this study, including 49 consecutive patients with AS and 29 control subjects. The analysis included the following serum biomarkers: thrombomodulin (TM), platelet factor 4 (PF4), P-selectin, and CD40L. The efficacy of antiplatelet treatment was evaluated using the VerifyNow Aspirin test (ASPI test) and P2Y12 assay test (ADP test). Patients with AS exhibited increased serum levels of TM (7.64 ± 3.5 ng/mL vs. 6.28 ± 2.1 ng/mL, p = 0.011) and PF4 (25.16; Q1: 8.3; Q3: 29.6 μg/mL vs. 12.85; Q1: 5.7; Q3: 14.5 μg/mL, p = 0.021) compared to the control group. P-selectin and CD40L levels did not differ between groups. There were no significant differences in platelet aggregation in the ASPI (474.04 ± 66.7 ARU vs. 471.31 ± 56.2 ARU; p = 0.822) or ADP (224.88 ± 46.4 PRU vs. 216.62 ± 29.6 PRU; p = 0.394) tests. Bleeding incidence did not differ significantly between groups. The coexistence of AS in patients with CAD is associated with elevated levels of the aforementioned biomarkers, which are indicative of endothelial damage and platelet activation. However, the efficacy of antiplatelet treatment was independent of the presence of AS. Full article

This work systematically addresses the dual challenges of non-inertial dynamic coupling and kinematic constraint redundancy encountered in dynamic modeling of serial–parallel–serial hybrid robotic mechanisms, and proposes an improved Newton–Euler modeling method with constraint compensation. Taking the Skiing Simulation Platform with 6-DOF as the research mechanism, the inverse kinematic model of the closed-chain mechanism is established through $G_F$ set theory, with explicit analytical expressions derived for the motion parameters of limb mass centers. Introducing a principal inertial coordinate system into the dynamics equations, a recursive algorithm incorporating force/moment coupling terms is developed. Numerical simulations reveal a 9.25% periodic deviation in joint moments using conventional methods. Through analysis of the mechanism’s intrinsic properties, it is identified that the lack of angular momentum conservation constraints on the end-effector in non-inertial frames leads to system controllability degradation. Accordingly, a constraint compensation strategy is proposed: establishing linearly independent differential algebraic equations supplemented with momentum/angular momentum balance equations for the end platform. Co-Simulation results demonstrate that the optimized model reduces the maximum relative error of actuator joint moments to 0.98%, and maintains numerical stability across the entire configuration space. The constraint compensation framework provides a universal solution for dynamics modeling of complex closed-chain mechanisms, validated through applications in flight simulators and automotive driving simulators. Full article

Polyoxometalates are a promising family of compounds for the development of new catalyst materials, although up to now they have mainly been applied in acid catalysis and oxidative processes. In this study, we present the synthesis and characterisation of two new Keggin-type phosphomolybdates, H₆[PRuMo₁₁O₄₀] and H₉[PRu₂Mo₁₀O₄₀]. The successful synthesis was confirmed with ICP-OES (elemental composition) and infrared spectroscopy (structure). Furthermore, the molecular structure of H₆[PRuMo₁₁O₄₀] was determined by electron diffraction. The new compounds were comprehensively characterised using ³¹P-NMR spectroscopy, UV-Vis spectroscopy, and electrochemical methods. Square-Wave-Voltammetry revealed an additional RedOx peak for the Ru-substituted POMs compared to the unsubstituted phosphomolybdate at around 825 mV. In a test reaction, the new compounds showed promising catalytic activity for the hydrogenation of lactic acid. Full article

Toxoplasma gondii (T. gondii) is an intracellular parasite that extensively infects warm-blooded animals, causing toxoplasmosis and posing a significant threat to global public health. In this study, we investigated the association between T. gondii infection and ferroptosis in host cells, as well as the regulatory role of glutathione peroxidase 4 (GPX4). Our findings revealed that mice infected with RH and PRU strains of T. gondii exhibited significantly elevated levels of reactive oxygen species and malondialdehyde in brain and liver tissues. Concurrently, the expression of GPX4, a critical negative regulator of ferroptosis, was downregulated, which correlated with the elevated parasite burden. In Vero cells, T. gondii infection similarly inhibited GPX4 expression, whereas GPX4 overexpression suppressed T. gondii proliferation. These results indicate that T. gondii infection can promote ferroptosis in host cells and that GPX4 plays a pivotal role in regulating infection and proliferation. This study provides novel insights into the pathogenic mechanisms of T. gondii and identifies GPX4 as a regulatory factor that constrains parasite proliferation, offering new approaches for toxoplasmosis prevention and control. Full article

The expression of members of the progesterone receptor membrane component (PGRMC) family, particularly PGRMC1, is elevated in diverse types of cancers, particularly those of the female reproductive system. While xenograft tumor studies using human transformed cell lines in immunocompromised mice have suggested that PGRMC1 enhances tumor growth and chemoresistance, the exact role of members of the PGRMC family in cancer development in vivo remains unclear. In this study, we examined the effect of deleting Pgrmc2 on the development of endometrial hyperplasia and cancer using a murine phosphatase and tensin homologue (Pten) conditional loss-of-function model. We previously established that PGRMCs are cell survival factors that are required for normal estrogen-induced uterine epithelial cell proliferation and normal female fertility. The deletion of Pgrmc2 reduced the incidence and severity of endometrial hyperplasia and cancer in mice with conditional Pten-heterozygous uteri and increased lifespan in mice with conditional Pten-knockout uteri. Mechanistically, the deletion of Pgrmc2 decreased uterine glandular epithelial cell proliferation. Pten loss-of-function-induced endometrial hyperplasia and cancer elevated uterine inflammation, but this was not impacted by PGRMC2 deficiency. This study identifies PGRMC2 as a potential therapeutic target to be inhibited in the treatment of endometrial hyperplasia and cancer, particularly where PTEN activity is reduced due to gene mutation or loss. Full article

The purpose of change detection is to recognize changed areas from a pair of two remote sensing images. However, since change areas often include multiple terrain features, this demands enhanced feature extraction capability from the model. This paper proposes a frozen-parameter Transformer self-attention change detection network (ZAQNet). The network integrates four innovative modules: a GIAU (Generalized Image Attention Unit) which can effectively fuse the features of two remote sensing images and accurately focus on changing areas; a GSAU (Global Spatial Attention Unit) which performs self attention processing in the image spatial dimension to enhance the model’s ability to capture global change information; a GSCU (Global Semantic Context Unit) which performs self-attention operations in the channel dimension to enhance the model’s attention to feature maps containing changing information; and a PRU (Patch Refinement Unit) which extracts and refines spatial position information from the underlying feature map, optimizing the restoration effect of the feature map. The experiments on the BTRS-CD and LEVIR-CD datasets show that ZAQNet performs excellently in change detection tasks. Among them, the change detection index F1 and IOU are better than the comparison model. These results fully demonstrate the superiority, robustness, and generalization ability of ZAQNet in change detection tasks and provide an efficient and reliable solution for remote sensing image analysis. Full article

This paper presents a resonant wireless power transfer method that leverages a 90-degree voltage phase shift between the transmitting and receiving coils to enhance efficiency and maximize power transfer. When the resonant coupling is achieved, the secondary coil with an adjustable capacitor forms a tuned LC circuit. If the primary coil is driven at the resonant frequency of both the primary and secondary sides, the system can transmit 250W of power between the coils over a distance of 50 cm. Using a single power transmitting unit (PTU) board with multiple paralleled gallium nitride high-electron-mobility transistors (GaN HEMTs), the system achieves a maximum power transfer efficiency of 88%, highlighting the effectiveness of the design in high-efficiency, long-distance wireless power transmission. The key to the success of high-power, high-efficiency RWPT is in exhibiting the imaginary turn ratio presented on the air transformer. The imaginary turn ratio can realize the negative impedance conversion that converts the positive resistance on the power-receiving unit into a negative one, and thus, the damping of the resonance oscillation becomes negative and positively encourages more power to be delivered to the power-receiving unit (PRU) load. This paper derives the theory of the imaginary turn ratio and demonstrates the implementation of the RWPT system that exhibits the imaginary turn ratio effect. Full article

Thaumatin-like proteins (TLPs) are a class of allergens that are predominantly found in fruits. These proteins are involved in plant defense mechanisms and exhibit antifungal activity. TLPs are classified as pathogenesis-related proteins (PR-5) and are typically synthesized in response to biotic stress, such as pathogen attacks. Sensitization to TLPs can result in a broad spectrum of allergic reactions, ranging from localized symptoms, such as oral allergy syndrome, to severe manifestations, including anaphylaxis. Key allergens within this group include Mal d 2 (apple), Pru p 2 (peach), and Pru av 2 (cherry). The list of allergens belonging to the TLP protein group continues to expand with newly discovered molecules. Diagnostic approaches for TLP allergies remain limited. Allergen component-resolved diagnostics can detect specific TLPs. The epidemiology of TLP-induced allergies is underexplored, and further research is needed to elucidate the prevalence, natural course, and clinical outcomes of these allergic conditions. Full article

Cry j 7 is a 7 kDa cysteine-rich gibberellin regulatory protein (GRP) with six disulfide bonds. It was isolated from Japanese cedar as the pollen allergen in this study. It exhibits cross-reactivity with food allergens such as Pru p 7 from peach and causes pollen-food allergy syndrome (PFAS). In this work, recombinant Cry j 7 and Pru p 7 were successfully overexpressed using Pichia pastoris in a high-cell-density fermentation culture, and pure proteins were purified by reverse-phase HPLC. The characterization of Cry j 7 and Pru p 7 were performed by MS, CD, and ¹H-NMR experiments to confirm the correct native conformation of Cry j 7 as well as Pru p 7. When compared, the results showed that Cry j 7 exhibits excellent stability in disulfide linkages and preserves its original structure up to 90 °C in various pH buffers in comparison to Pru p 7. Notably, NMR analyses indicated the greater mobility in the α-helix and loop regions of S38-C47 in Pru p 7 compared to those of Cry j 7. Furthermore, our results showed that the sensitivity of Cry j 7 to enzyme digestion differed from that of Pru p 7: Cry j 7 was more susceptible to proteolysis, while Pru p 7 displayed better resistance in the gastrointestinal tract. These variations in structural stability and sensitivity to proteolysis provide valuable insights into the allergenicity within the GRP family. Full article

Introduction: Lipid Transfer Proteins (LTPs) are plant-derived panallergens that have emerged as significant allergens in Mediterranean populations. Though less common in children, LTP allergies represent a critical consideration for physicians diagnosing plant food allergies in this demographic. Methodology: PRISMA-ScR guidelines were followed. A search with specific terms was performed in searchable databases. Two of the authors extracted and evaluated the data. Results: A total of 21 original studies and 6 case reports focusing on LTP allergies in the paediatric population met the inclusion criteria. Diagnostic tools, predictive markers and management options for LTP allergies were examined. Allergens, clinical presentation and history were the diagnostic tools investigated. The clinical and laboratory phenotypes of the patient were considered possible predictive markers for the evaluation and progression of LTP allergies. Lastly, dietary modifications and sublingual immunotherapy were identified as the main focus of LTP allergy management. Discussion: A summary of the results is presented, and at the same time, questions concerning the nature of LTP allergies and their management are raised. Conclusions: LTP allergy in children is something physicians should be aware of. Further research is needed to establish the differences in LTP allergies in children and adults and the effectiveness of immunotherapy in paediatric populations. Full article

Toxoplasma gondii is a widely spread opportunistic pathogen that can infect nearly all warm-blooded vertebrates and cause serious toxoplasmosis in immunosuppressed animals and patients. However, the relationship between the host’s innate immune system and effector proteins is poorly understood, particularly with regard to how effectors antagonize cGAS-STING signaling during T. gondii infection. In this study, the ROP5 from the PRU strain of T. gondii was found to promote cGAS-STING-mediated immune responses. Mechanistically, ROP5 interacted with STING through predicted domain 2 and modulated cGAS-STING signaling in a predicted domain 3-dependent manner. Additionally, ROP5 strengthened cGAS-STING signaling by enhancing the K63-linked ubiquitination of STING. Consistently, ROP5 deficient PRU (PRUΔROP5) induced fewer type I IFN-related immune responses and replicated faster than the parental strain in RAW264.7 cells. Taken together, this study provides new insights into the mechanism by which ROP5 regulates T. gondii infection and provides new clues for strategies to prevent and control toxoplasmosis. Full article

Background/Objectives: Low quality diets are a risk factor for non-communicable diseases; therefore, improving diet quality is a public health and policy priority in the UK and elsewhere. Reformulating food/beverage products to make them healthier may be an effective approach. Evidence suggests that fiscal interventions, notably taxes/levies on soft drinks, can lead to reformulation but the evidence for voluntary or mandated non-fiscal interventions is less clear. We aimed to review and synthesise contemporary evidence to determine whether non-fiscal policies/interventions result in the reformulation of food/beverage products Methods: In April 2023, we systematically searched ten international academic and nine grey literature databases. We included real-world study designs, all nutrients, in- and out-of-home sectors, and studies published from 2013, to ensure policy relevancy. We excluded modelling studies. Using the Synthesis Without Meta-Analysis method we conducted vote counting of studies based on the direction of effect and narrative synthesis by intervention type. Risk of bias was assessed using a tool developed by the EPPI-Centre and quality was assessed using GRADE. Results: We included 77 real-world studies from 19 countries, reporting 100 non-fiscal policies/interventions. Most commonly, these were reduction targets (n = 44), front-of-pack labels (n = 23), and advertising standards (n = 9). Most interventions were voluntary (n = 67), compared to mandatory (n = 33), and focused on the in-home sector (n = 63). The vote counting results showed non-fiscal policies/interventions overall led to improvements in reformulation in 60/63 studies with a valid direction of effect (95%, 95% CI 0.869, 0.984, p < 0.001). Mandatory implementations were more successful than voluntary implementations with 15/15 showing an improvement (100%, 95% CI 0.796, 1], p < 0.001), compared 40/43 showing an improvement (93%, 95% CI 0.814, 0.976, p < 0.001). Most of the studies were of low quality, due to the observational nature of the studies. Sodium was the most commonly targeted nutrient (n = 56) and was found to be reformulated in most studies. Causation is difficult to establish from real-world studies, but evidence suggests that regulatory and multi-component strategies may be effective at driving reformulation. Conclusions: Non-fiscal policies/interventions can play an important role in driving reformulation, alongside fiscal measures. This work was funded by the National Institute for Health and Care Research PRP-PRU-02-15-Healthy Weight and registered on Open Science Framework. Full article

Clopidogrel, a prescription drug to reduce ischemic events in cardiovascular patients, has been extensively studied in mostly European individuals but not among Caribbean Hispanics. This study evaluated the low abundance and reduced activity of paraoxonase-1 (PON1) in clopidogrel-resistant patients as a predictive risk biomarker of poor responders and disease severity in this population. Thirty-six patients on clopidogrel (cases divided into poor and normal responders) were enrolled, along with 11 cardiovascular patients with no clopidogrel indications (positive control) and 13 healthy volunteers (negative control). Residual on-treatment platelet reactivity unit (PRU), PON1 abundance by Western blotting, and PON1 activity by enzymatic assays were measured. PON1 genotyping and computational haplotype phasing were performed on 512 DNA specimens for two genetic loci (rs662 and rs854560). No statistical differences in mean relative PON1 abundance were found among the groups (p > 0.05). However, a significantly lower enzymatic activity was found in poor responders (10.57 ± 6.79 µU/mL) when compared to controls (22.66 ± 8.30 µU/mL and 22.21 ± 9.66 µU/mL; p = 0.004). PON1 activity among carriers of the most prevalent PON1 haplotype (AA|AA) was significantly lower than in wild types (7.90 µU/mL vs. 22.03 µU/mL; p = 0.005). Our findings suggested that PON1 is a potential biomarker of cardiovascular disease severity in Caribbean Hispanics. Full article

This article presents a methodology for analyzing probabilistic air traffic complexity by integrating prediction uncertainties in convective weather scenarios. With the Performance Review Unit (PRU) model as a base, this method modifies the original framework by incorporating a weather-related complexity indicator. The approach was tested in Austrian airspace using ensemble weather forecasts and historical flight plan data. The results demonstrated that a probabilistic model effectively assesses traffic complexity and captures trends in complexity over time, providing greater reliability in high-complexity sectors. Validation revealed a strong alignment between simulator complexity values and probabilistic complexity, especially in sectors characterized by dense data distributions. In contrast, sectors with more elongated distributions tended to overestimate complexity. Quantitative analysis indicated that the error between the probabilistic mean complexity and the simulator complexity values ranged from 12% to 23%, with higher errors in sectors with lower complexity. This validation confirmed the model’s ability to predict complexity trends, thereby assisting flow manager positions (FMPs) in traffic flow and airspace management. Overall, this study demonstrated that probabilistic complexity assessment provides a deeper understanding of traffic behaviour, facilitating more effective air traffic flow management in uncertain and dynamic conditions. Full article

In the Internet of Things (IoT), the selection of mobile users with IoT-enabled devices plays a crucial role in ensuring the efficiency and accuracy of data collection. The reputation of these mobile users is a key indicator in selecting high-quality participants, as it directly reflects the reliability of the data they submit and their past performance. However, existing approaches often rely on a trusted centralized server, which can lead to single points of failure and increased vulnerability to attacks. Additionally, they may not adequately address the potential manipulation of reputation scores by malicious entities, leading to unreliable and potentially compromised user selection. To address these challenges, we propose PRUS, a privacy-preserving and quality-aware user selection scheme for IoT. By leveraging the decentralized and immutable nature of the blockchain, PRUS enhances the reliability of the user selection process. The scheme utilizes a public-key cryptosystem with distributed decryption to protect the privacy of users’ data and reputation, while truth discovery techniques are employed to ensure the accuracy of the collected data. Furthermore, a privacy-preserving verification algorithm using reputation commitment is developed to safeguard against the malicious tampering of reputation scores. Finally, the Dirichlet distribution is used to predict future reputation values, further improving the robustness of the selection process. Security analysis demonstrates that PRUS effectively protects user privacy, and experimental results indicate that the scheme offers significant advantages in terms of communication and computational efficiency. Full article